SMED30006970

Overview


Name	SMED30006970
Smed ID	SMED30006970
Length (bp)	1489

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30006970 (SMED30006970) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006970 (SMED30006970) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006970 (SMED30006970) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006970 (SMED30006970) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30006970

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30006970	SMESG000009578.1	SmedASXL_013698	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30006970	SMESG000009578.1	SmedASXL_013698	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
nervous system	SMED30006970	SMESG000009578.1	dd_Smed_v4_3566_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30006970	SMESG000009578.1	dd_Smed_v4_3566_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30006970	SMESG000009578.1	dd_Smed_v4_3566_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30006970	SMESG000009578.1	dd_Smed_v4_3566_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30006970 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000310:72961..74774 +	7099

Homology

[Close]

BLAST of SMED30006970 vs. Ensembl Human
Match: LEO1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:30401])

HSP 1 Score: 268.085 bits (684), Expect = 1.289e-81
Identity = 143/244 (58.61%), Postives = 188/244 (77.05%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWRI +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    +ISLA IK +
Sbjct:  358 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNR 593

[Close]

BLAST of SMED30006970 vs. Ensembl Human
Match: LEO1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:30401])

HSP 1 Score: 154.451 bits (389), Expect = 3.669e-40
Identity = 83/147 (56.46%), Postives = 114/147 (77.55%), Query Frame = -2

Query:  253 SLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 687
            S+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    +ISLA IK +
Sbjct:  387 SMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNR 533

[Close]

BLAST of SMED30006970 vs. Ensembl Celegans
Match: leo-1 (RNA polymerase-associated protein LEO1 [Source:UniProtKB/Swiss-Prot;Acc:Q17431])

HSP 1 Score: 191.815 bits (486), Expect = 1.876e-55
Identity = 89/184 (48.37%), Postives = 137/184 (74.46%), Query Frame = -2

Query:  385 HLVKLPNFLSVETRPFDPMYYE-DEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADH-NHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASL 930
            H V++PNFLSV T PFDP +YE DE DE    D+EG+TRLKL+VENT+RWR+ KD  G+ I        RESNA++++W DG++SL+LG EIF+V    +++++  HL+V++ + + AQA+ T ++ FRPHSTDS THRK+TL++AD++ K A+VK++   G+NP++ ++E  ++EEE ++A +
Sbjct:  167 HFVRMPNFLSVATHPFDPQHYEEDEDDEQAKLDDEGRTRLKLRVENTLRWRVRKDENGKEI--------RESNAKIVKWDDGTMSLYLGNEIFEVSLVPLNSNNLPHLYVKQPTLMSAQAVLTHRMTFRPHSTDSQTHRKVTLNMADRSRKNAQVKVMDDVGQNPEITRRENARKEEESLRAHI 342

[Close]

BLAST of SMED30006970 vs. Ensembl Fly
Match: Atu (gene:FBgn0019637 transcript:FBtr0078704)

HSP 1 Score: 238.81 bits (608), Expect = 1.700e-70
Identity = 119/248 (47.98%), Postives = 171/248 (68.95%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXREL-----TAAFL-EQQKDDENNPHAISLAEIKKK 978
            I+V+ P I ADLG+E H +KLPNFLSV T PFDP  YEDE+DE E  DEEG+ R+KLKV NTIRWR   +++G  +        RESNAR +RWSDGS+SLHLG EIFD +   +  DHNHLFVR+G+GLQ Q++F +KL FRPHST+SFTH+K+T+SLAD++ K + +K+L   G++P   +   ++ EE +++ ++R +++    +++    E      T+++  ++  DDEN   AISL+ IK +
Sbjct:  380 IDVEIPRISADLGKEQHFIKLPNFLSVVTHPFDPETYEDEIDEEETMDEEGRQRIKLKVSNTIRWREYMNNKGDMV--------RESNARFVRWSDGSMSLHLGNEIFDAYRQPLLGDHNHLFVRQGTGLQGQSVFRTKLTFRPHSTESFTHKKMTMSLADRSSKTSGIKILTQVGKDPTTDRPTQLREEEAKLRQAMRNQHKSLPKKKKPGAGEPLIGGGTSSYQHDEGSDDEN---AISLSAIKNR 616

[Close]

BLAST of SMED30006970 vs. Ensembl Fly
Match: CG10887 (gene:FBgn0038773 transcript:FBtr0083864)

HSP 1 Score: 107.071 bits (266), Expect = 3.186e-24
Identity = 54/181 (29.83%), Postives = 101/181 (55.80%), Query Frame = -2

Query:  394 HLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSF--THRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMK 930
              +K+P F+ VE++ + P  ++D M +++++DE+ +     +++ T+RWR            EN+   +ESNA+++RWSDGS + H+G E+FD+    ++ + NHL+VR GS  Q Q +  +KL  RP    +F  +H +   + A    ++  VK++   G NP+      +K E  +++
Sbjct:  395 QFLKMPYFIPVESKAYVPETFQDRMTKNDLKDEQSREDFINRLKATVRWR------------ENENKTKESNAKIVRWSDGSETFHVGNEVFDMMHHPVTVNQNHLYVRLGSFYQPQGLIQNKLTVRPMLDSNFGQSHVQALRNRATNKPQSGCVKVITNMGSNPEHDHDRRMKEELSKLR 563

[Close]

BLAST of SMED30006970 vs. Ensembl Zebrafish
Match: leo1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:404599])

HSP 1 Score: 270.011 bits (689), Expect = 2.511e-82
Identity = 142/256 (55.47%), Postives = 192/256 (75.00%), Query Frame = -2

Query:  217 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKKARPSSSIKKKKT 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG  +        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L A +LE  +  +DE    +ISLA IK K +    +++++ 
Sbjct:  336 IEVEIPKVSTDLGSDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRSRRDEEGNEV--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRNEMIKKEEERLRASIRRESQQRRMREKQHQRGLNAGYLEPDRYDEDEEGEESISLAAIKSKYKGGGGLREERA 583

[Close]

BLAST of SMED30006970 vs. Ensembl Xenopus
Match: LEO1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:100135118])

HSP 1 Score: 268.47 bits (685), Expect = 3.674e-83
Identity = 143/244 (58.61%), Postives = 187/244 (76.64%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  KD EG  +        RESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A +LE  +  D+E    +ISLA IK +
Sbjct:  245 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRTRKDEEGSDV--------RESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRSEMIKKEEERLRASIRRESQQRRMREKQHQRGLSANYLEPDRYDDEEEGEESISLAAIKNR 480

[Close]

BLAST of SMED30006970 vs. Ensembl Xenopus
Match: LEO1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:100135118])

HSP 1 Score: 270.396 bits (690), Expect = 1.198e-82
Identity = 143/244 (58.61%), Postives = 187/244 (76.64%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  KD EG  +        RESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A +LE  +  D+E    +ISLA IK +
Sbjct:  370 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRTRKDEEGSDV--------RESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRSEMIKKEEERLRASIRRESQQRRMREKQHQRGLSANYLEPDRYDDEEEGEESISLAAIKNR 605

[Close]

BLAST of SMED30006970 vs. Ensembl Xenopus
Match: LEO1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:100135118])

HSP 1 Score: 270.781 bits (691), Expect = 3.119e-82
Identity = 143/244 (58.61%), Postives = 187/244 (76.64%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  KD EG  +        RESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A +LE  +  D+E    +ISLA IK +
Sbjct:  401 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRTRKDEEGSDV--------RESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRSEMIKKEEERLRASIRRESQQRRMREKQHQRGLSANYLEPDRYDDEEEGEESISLAAIKNR 636

[Close]

BLAST of SMED30006970 vs. Ensembl Mouse
Match: Leo1 (Leo1, Paf1/RNA polymerase II complex component [Source:MGI Symbol;Acc:MGI:2685031])

HSP 1 Score: 268.47 bits (685), Expect = 7.099e-82
Identity = 142/244 (58.20%), Postives = 188/244 (77.05%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWRI +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    ++SLA IK +
Sbjct:  359 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESVSLAAIKNR 594

[Close]

BLAST of SMED30006970 vs. UniProt/SwissProt
Match: sp|Q6NYV9|LEO1_DANRE (RNA polymerase-associated protein LEO1 OS=Danio rerio OX=7955 GN=leo1 PE=1 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 1.896e-81
Identity = 142/256 (55.47%), Postives = 192/256 (75.00%), Query Frame = -2

Query:  217 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKKARPSSSIKKKKT 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG  +        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L A +LE  +  +DE    +ISLA IK K +    +++++ 
Sbjct:  336 IEVEIPKVSTDLGSDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRSRRDEEGNEV--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRNEMIKKEEERLRASIRRESQQRRMREKQHQRGLNAGYLEPDRYDEDEEGEESISLAAIKSKYKGGGGLREERA 583

[Close]

BLAST of SMED30006970 vs. UniProt/SwissProt
Match: sp|Q52KV5|LEO1_XENLA (RNA polymerase-associated protein LEO1 OS=Xenopus laevis OX=8355 GN=leo1 PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 3.332e-81
Identity = 142/244 (58.20%), Postives = 187/244 (76.64%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  KD EG  +        R+SNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A +LE  +  D+E    +ISLA IK +
Sbjct:  389 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRTRKDEEGNDV--------RDSNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRSEMIKKEEERLRASIRRESQQRRMREKQHQRGLSANYLEPDRYDDEEEGDESISLAAIKNR 624

[Close]

BLAST of SMED30006970 vs. UniProt/SwissProt
Match: sp|Q5XJE5|LEO1_MOUSE (RNA polymerase-associated protein LEO1 OS=Mus musculus OX=10090 GN=Leo1 PE=1 SV=2)

HSP 1 Score: 268.47 bits (685), Expect = 4.966e-81
Identity = 142/244 (58.20%), Postives = 188/244 (77.05%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWRI +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    ++SLA IK +
Sbjct:  359 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESVSLAAIKNR 594

[Close]

BLAST of SMED30006970 vs. UniProt/SwissProt
Match: sp|Q8WVC0|LEO1_HUMAN (RNA polymerase-associated protein LEO1 OS=Homo sapiens OX=9606 GN=LEO1 PE=1 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 6.190e-81
Identity = 143/244 (58.61%), Postives = 188/244 (77.05%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWRI +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    +ISLA IK +
Sbjct:  358 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNR 593

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BLAST of SMED30006970 vs. UniProt/SwissProt
Match: sp|Q641X2|LEO1_RAT (RNA polymerase-associated protein LEO1 OS=Rattus norvegicus OX=10116 GN=Leo1 PE=1 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 2.358e-80
Identity = 141/244 (57.79%), Postives = 188/244 (77.05%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDD--ENNPHAISLAEIKKK 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR+ +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+ ++ EMIK+EEER++AS+RRE+Q+RRMRE+   R L+A++LE  + D  E    ++SLA IK +
Sbjct:  370 IEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRMRRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESVSLAAIKNR 605

[Close]

BLAST of SMED30006970 vs. TrEMBL
Match: A0A0X3NP53 (RNA polymerase-associated protein LEO1 (Fragment) OS=Schistocephalus solidus OX=70667 GN=LEO1 PE=4 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 1.498e-89
Identity = 156/261 (59.77%), Postives = 195/261 (74.71%), Query Frame = -2

Query:  226 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXX-----XXXXXXRELTAAFLEQQKDDENN---PHAISLAEIKK--KARPSSSIKK 978
            I V FP+I+ DLG EMHLVK+PNFLSVETRPFDP  YEDE+DE EV DEEG+TRLKLKVENTIRWR+ K+  G+ I         ESNAR++RWSDGSLSL+LG EIFDVH  DI +D NHLF+REGSGLQ QA+F +KL FRPHST+SFTHRK+TLSLADKT+K+ KVK+LP AG +P+  +  ++++EEER++ASLRRE+Q+RRM        A     ++  +    DDEN+     +ISLA IK+  KA  SSS ++
Sbjct:  208 IAVAFPLIRVDLGREMHLVKMPNFLSVETRPFDPAVYEDELDEDEVLDEEGRTRLKLKVENTIRWRLGKNEAGETI--------HESNARIVRWSDGSLSLYLGNEIFDVHKMDIQSDFNHLFIREGSGLQGQAVFKTKLTFRPHSTESFTHRKITLSLADKTNKSQKVKILPVAGADPESNRHALVRKEEERLRASLRRESQQRRMRERQLASAAHRNRRSSLTVSGYGDDENSLDEGTSISLAAIKRDTKAGRSSSTRR 460

[Close]

BLAST of SMED30006970 vs. TrEMBL
Match: R7UT86 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_221877 PE=4 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 1.297e-88
Identity = 153/247 (61.94%), Postives = 193/247 (78.14%), Query Frame = -2

Query:  238 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDDENNPHAISLAEIKKKARPSS 978
            IEV+ P I   LG+ ++ VKLPNFLSVETRPFDP  YEDE+DE EV DEEG+TRLKLKVENTIRWR  KD +G  +LDE  +P RESNAR +RWSDGS+S+HLG EIFDVH   +  D NHLF+R+G+GLQ QA+F +KL FRPHSTDSFTHRK+T+S+ D++ K  KVK+LP AG++P+ ++ EMIK+EEER++AS+RRE+Q+RR+RERA  R L   +LE   DDEN    ISL+ IK K + S+
Sbjct:  301 IEVEIPKINTALGKNLYFVKLPNFLSVETRPFDPQVYEDEIDEDEVMDEEGRTRLKLKVENTIRWRTMKDDDGNDVLDEFGEPVRESNARYVRWSDGSMSMHLGNEIFDVHPMTLQGDFNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSFTHRKMTMSMVDRSSKTQKVKVLPIAGKDPESQRSEMIKKEEERLRASIRRESQQRRIRERAHQRGLNPNYLEDD-DDEN---GISLSAIKHKYKQSA 543

[Close]

BLAST of SMED30006970 vs. TrEMBL
Match: A0A3P7YK29 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS1001 PE=4 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 2.508e-88
Identity = 129/198 (65.15%), Postives = 162/198 (81.82%), Query Frame = -2

Query:  385 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASL 978
            I VDFP+I+ADLG E+HLVK+PNFLSVETRPFDP +YEDE+DE E+ DEEG+TRLKLKVENTIRWRI K  +G+ I         ESNAR++RWSDGSLSLHLG EIFD+H  DI +D+N+LF+REGSGLQ Q+   +KL FRPHSTDSFTHRK+TLSLADKT+K  KVK+LP  G +P+  +  ++++EEE+++A+L
Sbjct:  104 ISVDFPLIRADLGREVHLVKMPNFLSVETRPFDPNFYEDELDEDEILDEEGRTRLKLKVENTIRWRIGKTPDGEMI--------HESNARIVRWSDGSLSLHLGDEIFDIHKVDIQSDYNYLFIREGSGLQGQSSLKTKLTFRPHSTDSFTHRKITLSLADKTNKLQKVKILPVTGADPESNRNVLVRKEEEKLRATL 293

[Close]

BLAST of SMED30006970 vs. TrEMBL
Match: A0A183JE88 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 PE=4 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 2.647e-88
Identity = 129/198 (65.15%), Postives = 162/198 (81.82%), Query Frame = -2

Query:  385 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASL 978
            I VDFP+I+ADLG E+HLVK+PNFLSVETRPFDP +YEDE+DE E+ DEEG+TRLKLKVENTIRWRI K  +G+ I         ESNAR++RWSDGSLSLHLG EIFD+H  DI +D+N+LF+REGSGLQ Q+   +KL FRPHSTDSFTHRK+TLSLADKT+K  KVK+LP  G +P+  +  ++++EEE+++A+L
Sbjct:  104 ISVDFPLIRADLGREVHLVKMPNFLSVETRPFDPNFYEDELDEDEILDEEGRTRLKLKVENTIRWRIGKTPDGEMI--------HESNARIVRWSDGSLSLHLGDEIFDIHKVDIQSDYNYLFIREGSGLQGQSSLKTKLTFRPHSTDSFTHRKITLSLADKTNKLQKVKILPVTGADPESNRNVLVRKEEEKLRATL 293

[Close]

BLAST of SMED30006970 vs. TrEMBL
Match: A0A2G8LCE8 (Putative RNA polymerase-associated protein LEO1 isoform X1 OS=Stichopus japonicus OX=307972 GN=BSL78_05205 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 2.767e-88
Identity = 145/242 (59.92%), Postives = 186/242 (76.86%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDDENNPHAISLAEIKKK 978
            I V+ P I  DLGEE H VKLPNFLSVETRPFDP +YEDE D+ EV DEEG+TRLKLK+ENTIRWR   D EG  I        +ESNAR++RWSDGSLSLHLG E+FDV+ T I  D+NHLF+R+G+GLQ   +FT+KL FRPHSTDS THRK+TLS+ADKT K  K+++LP AG NP++ ++EMIK E+ER++A +RREN+++R+RE +  R L+A +LE  +D+E    +ISL+ IK +
Sbjct:  258 INVELPKINVDLGEEQHFVKLPNFLSVETRPFDPQFYEDEFDDDEVLDEEGRTRLKLKMENTIRWRKVFDEEGSEI-------GKESNARIVRWSDGSLSLHLGDEVFDVYKTTIQGDYNHLFIRQGTGLQGLGVFTTKLSFRPHSTDSATHRKMTLSMADKTQKVQKIRVLPMAGTNPEIHREEMIKAEDERLRAQMRRENKQKRVREYSHGRGLSANYLEPDRDEEGEDGSISLSAIKNR 492

[Close]

BLAST of SMED30006970 vs. Ensembl Cavefish
Match: leo1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:404599])

HSP 1 Score: 191.815 bits (486), Expect = 3.778e-54
Identity = 121/256 (47.27%), Postives = 169/256 (66.02%), Query Frame = -2

Query:  217 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKKARPSSSIKKKKT 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FR   T + TH   T   A    +   + L          +  E +++EEER++AS+RRE+Q+RRMRE+   R L+  +LE  +  +DE    ++SLA IK K +    +++++ 
Sbjct:  268 IEVEIPKVSTDLGSDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRARRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFR--YTHTHTH---TFIFAITKQRTEMIHL------KHTHQHTEKLRKEEERLRASIRRESQQRRMREKQHQRGLSTGYLEPDRYEEDEEGEESVSLAAIKSKYKGGGGLREERA 504

[Close]

BLAST of SMED30006970 vs. Ensembl Cavefish
Match: leo1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:404599])

HSP 1 Score: 188.348 bits (477), Expect = 9.206e-53
Identity = 119/256 (46.48%), Postives = 162/256 (63.28%), Query Frame = -2

Query:  217 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKKARPSSSIKKKKT 978
            IEV+ P +  DLG +++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG  I        +ESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FR      +TH          TH                      I +EEER++AS+RRE+Q+RRMRE+   R L+  +LE  +  +DE    ++SLA IK K +    +++++ 
Sbjct:  306 IEVEIPKVSTDLGSDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRARRDEEGNEI--------KESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFR------YTH----------TH------------------THTFIFKEEERLRASIRRESQQRRMREKQHQRGLSTGYLEPDRYEEDEEGEESVSLAAIKSKYKGGGGLREERA 519

[Close]

BLAST of SMED30006970 vs. Ensembl Cavefish
Match: leo1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:404599])

HSP 1 Score: 167.548 bits (423), Expect = 3.962e-46
Identity = 84/143 (58.74%), Postives = 108/143 (75.52%), Query Frame = -2

Query:  550 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFR 978
            IEV+ P +  DLG +++ VKLPNFLSVE  PFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG  I        +ESNAR+++WSDG +SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL F+
Sbjct:  193 IEVEIPKVSTDLGSDLYFVKLPNFLSVEP-PFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRARRDEEGNEI--------KESNARIVKWSDG-MSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFK 325

[Close]

BLAST of SMED30006970 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007677.1 (pep scaffold:Pmarinus_7.0:GL476395:350535:368781:1 gene:ENSPMAG00000006921.1 transcript:ENSPMAT00000007677.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 281.952 bits (720), Expect = 9.115e-88
Identity = 143/245 (58.37%), Postives = 186/245 (75.92%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK---DDENNPHAISLAEIKKK 978
            IEV+ P +  DLG E++ VKLPNFLSVETRPFDP YYEDE ++ E  DEEG+TRLKLKVENTIRWR  +D +G  +        +ESNAR IRWSDGS+SLHLG E+FDVH   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG++P+ ++ EMIK+EEER++AS+RRE+Q+RR RE+   R L++ +LE  +   ++E    AISLA IK +
Sbjct:  372 IEVEIPKVNTDLGNELYFVKLPNFLSVETRPFDPQYYEDEFEDDETMDEEGRTRLKLKVENTIRWRTIRDDDGNEV--------KESNARFIRWSDGSMSLHLGNEVFDVHKAPLQGDHNHLFIRQGTGLQGQAVFRTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPIAGKDPESQRTEMIKKEEERLRASIRRESQQRRQREKQHMRGLSSNYLESDRFEEEEEEGEEAISLAAIKNR 608

[Close]

BLAST of SMED30006970 vs. Ensembl Yeast
Match: LEO1 (Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay [Source:SGD;Acc:S000005649])

HSP 1 Score: 79.7221 bits (195), Expect = 1.776e-16
Identity = 55/202 (27.23%), Postives = 105/202 (51.98%), Query Frame = -2

Query:  394 KADLGE-----EMHLVKLPNFLSVETRPFDPMYYEDEMDE--SEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTD-------ISADHNHLF-VREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMK 954
            KA++ E     E+   ++PNFL+++  PFDP  +E +++E  S     E +   +L  ENT+RWR ++D + Q +        +ESN ++++WSDG+ SL +G E  D+   D       +S D   L    EG  ++   +F       P ST+S  H+KL+ ++  +  + +K         +P+V ++E+ +++ + ++
Sbjct:  156 KANVNETASHNEIFYARIPNFLTIDPIPFDPPSFEAKVNERASNSASREDQLDDRLIDENTVRWRYSRDKD-QHVF-------KESNTQIVQWSDGTYSLKVGEECTDILVNDTSNTFLTVSHDQQELIQCYEGGEIKKTLMFI------PTSTNSKIHQKLSKAVIRRNQRQSKGPGTYIVSMDPEVEKKELERKQSQILR 343

[Close]

BLAST of SMED30006970 vs. Ensembl Nematostella
Match: EDO44827 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUX9])

HSP 1 Score: 254.218 bits (648), Expect = 3.301e-79
Identity = 134/242 (55.37%), Postives = 180/242 (74.38%), Query Frame = -2

Query:  253 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDDENNPHAISLAEIKKK 978
            I+V  P  +  LG E++  KLPNFLS+ET+PFDP  YED++++ E+ DEEG+ RLKLKVENTIRWR  K S+   I        +ESNAR++RWSDGSLSL +G E+FD+  T I  D NHLFV++G+GLQ QA+F  KL FRPHST S THRK+TLS+AD+  KA K+K+LP AG++P+ ++ EMIK+EEER+++ LR ENQ+RRMRER+  R L +++LE  +DD+ +    SL +IKKK
Sbjct:  150 IDVTIPYCRVALGSELYFSKLPNFLSLETKPFDPALYEDDVEDDEILDEEGRARLKLKVENTIRWRYGKYSDDNEI--------KESNARIVRWSDGSLSLLVGAEVFDIQKTRIEGDFNHLFVQQGTGLQGQAVFKEKLTFRPHSTASQTHRKMTLSIADRALKAQKIKMLPAAGQDPEAQRSEMIKKEEERLRSQLRLENQQRRMRERSQARGLNSSYLEAGEDDDEDLEQ-SLLKIKKK 382

[Close]

BLAST of SMED30006970 vs. Ensembl Medaka
Match: leo1 (LEO1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:101154930])

HSP 1 Score: 271.552 bits (693), Expect = 1.612e-83
Identity = 145/256 (56.64%), Postives = 192/256 (75.00%), Query Frame = -2

Query:  217 IEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQK--DDENNPHAISLAEIKKKARPSSSIKKKKT 978
            IEV+ P +  DLG E++ VKLPNFLSVE RPFDP YYEDE ++ E+ DEEG+TRLKLKVENTIRWR  +D EG           RESNAR+++WSDGS+SLHLG E+FDV+   +  DHNHLF+R+G+GLQ QA+F +KL FRPHSTDS THRK+TLSLAD+  K  K+++LP AG +P+  + EMIK+EEER++AS+RRE+Q+RRMRER   R L++++LE  +  D+E    AISLA IK K +    +++++ 
Sbjct:  316 IEVEIPKVCTDLGSELYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRAKRDEEGNET--------RESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESHRNEMIKKEEERLRASIRRESQQRRMRERQHQRGLSSSYLEPDRYDDEEEGDEAISLAAIKSKYKGGGGLREERA 563

[Close]

BLAST of SMED30006970 vs. Planmine SMEST
Match: SMESG000009578.1 (SMESG000009578.1)

HSP 1 Score: 670.618 bits (1729), Expect = 0.000e+0
Identity = 437/438 (99.77%), Postives = 437/438 (99.77%), Query Frame = -2

Query:   97 MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLXXXXXXXXXXXXXXNHTAKXXXXXXXXXXXXXXXXLTQKDVFGSDTDEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNXXXXXXXXXXXXXXXGAIEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIXXXXXXXXXXXXXXXXXDXXXXXXXXXPRIISDDESFSE 1410
            MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLSSAEESENENIKKSNHTAKSLGLDSNDSEDSNDANLTQKDVFGSDTDEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNEDVPREPEPDEVGDDGAIEVDFPVIKADL EEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLRRENQRRRMRERASHRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIDMSSTDEEEEESMSDFSDPPPKKKGKKPRIISDDESFSE
Sbjct:    1 MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLSSAEESENENIKKSNHTAKSLGLDSNDSEDSNDANLTQKDVFGSDTDEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNEDVPREPEPDEVGDDGAIEVDFPVIKADLSEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLRRENQRRRMRERASHRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIDMSSTDEEEEESMSDFSDPPPKKKGKKPRIISDDESFSE 438

[Close]

BLAST of SMED30006970 vs. Planmine SMEST
Match: SMESG000009578.1 (SMESG000009578.1)

HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0
Identity = 437/439 (99.54%), Postives = 437/439 (99.54%), Query Frame = -2

Query:   97 MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLXXXXXXXXXXXXXXNHTAKXXXXXXXXXXXXXXXXLTQKDVFGSDT-DEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNXXXXXXXXXXXXXXXGAIEVDFPVIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXXXXXXXXRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIXXXXXXXXXXXXXXXXXDXXXXXXXXXPRIISDDESFSE 1410
            MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLSSAEESENENIKKSNHTAKSLGLDSNDSEDSNDANLTQKDVFGSDT DEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNEDVPREPEPDEVGDDGAIEVDFPVIKADL EEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLRRENQRRRMRERASHRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIDMSSTDEEEEESMSDFSDPPPKKKGKKPRIISDDESFSE
Sbjct:    1 MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLSSAEESENENIKKSNHTAKSLGLDSNDSEDSNDANLTQKDVFGSDTEDEDKVKDKRAANIIKEIIVGSDESGPEGNQTYQTQFDNEDVPREPEPDEVGDDGAIEVDFPVIKADLSEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLKLKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGREIFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLRRENQRRRMRERASHRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTIDMSSTDEEEEESMSDFSDPPPKKKGKKPRIISDDESFSE 439

The following BLAST results are available for this feature:

BLAST of SMED30006970 vs. Ensembl Human
BLAST of SMED30006970 vs. Ensembl Celegans
BLAST of SMED30006970 vs. Ensembl Fly
BLAST of SMED30006970 vs. Ensembl Zebrafish
BLAST of SMED30006970 vs. Ensembl Xenopus
BLAST of SMED30006970 vs. Ensembl Mouse
BLAST of SMED30006970 vs. UniProt/SwissProt
BLAST of SMED30006970 vs. TrEMBL
BLAST of SMED30006970 vs. Ensembl Cavefish
BLAST of SMED30006970 vs. Ensembl Sea Lamprey
BLAST of SMED30006970 vs. Ensembl Yeast
BLAST of SMED30006970 vs. Ensembl Nematostella
BLAST of SMED30006970 vs. Ensembl Medaka
BLAST of SMED30006970 vs. Planmine SMEST

BLAST of SMED30006970 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
LEO1	1.289e-81	58.61	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]
LEO1	3.669e-40	56.46	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]

BLAST of SMED30006970 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
leo-1	1.876e-55	48.37	RNA polymerase-associated protein LEO1 [Source:Un...	[more]

BLAST of SMED30006970 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Atu	1.700e-70	47.98	gene:FBgn0019637 transcript:FBtr0078704	[more]
CG10887	3.186e-24	29.83	gene:FBgn0038773 transcript:FBtr0083864	[more]

BLAST of SMED30006970 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
leo1	2.511e-82	55.47	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]

BLAST of SMED30006970 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
LEO1	3.674e-83	58.61	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]
LEO1	1.198e-82	58.61	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]
LEO1	3.119e-82	58.61	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]

BLAST of SMED30006970 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Leo1	7.099e-82	58.20	Leo1, Paf1/RNA polymerase II complex component [So...	[more]

BLAST of SMED30006970 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q6NYV9\|LEO1_DANRE	1.896e-81	55.47	RNA polymerase-associated protein LEO1 OS=Danio re...	[more]
sp\|Q52KV5\|LEO1_XENLA	3.332e-81	58.20	RNA polymerase-associated protein LEO1 OS=Xenopus ...	[more]
sp\|Q5XJE5\|LEO1_MOUSE	4.966e-81	58.20	RNA polymerase-associated protein LEO1 OS=Mus musc...	[more]
sp\|Q8WVC0\|LEO1_HUMAN	6.190e-81	58.61	RNA polymerase-associated protein LEO1 OS=Homo sap...	[more]
sp\|Q641X2\|LEO1_RAT	2.358e-80	57.79	RNA polymerase-associated protein LEO1 OS=Rattus n...	[more]

BLAST of SMED30006970 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0X3NP53	1.498e-89	59.77	RNA polymerase-associated protein LEO1 (Fragment) ...	[more]
R7UT86	1.297e-88	61.94	Uncharacterized protein OS=Capitella teleta OX=283...	[more]
A0A3P7YK29	2.508e-88	65.15	Uncharacterized protein OS=Schistosoma curassoni O...	[more]
A0A183JE88	2.647e-88	65.15	Uncharacterized protein OS=Schistosoma curassoni O...	[more]
A0A2G8LCE8	2.767e-88	59.92	Putative RNA polymerase-associated protein LEO1 is...	[more]

BLAST of SMED30006970 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
leo1	3.778e-54	47.27	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]
leo1	9.206e-53	46.48	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]
leo1	3.962e-46	58.74	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]

BLAST of SMED30006970 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000007677.1	9.115e-88	58.37	pep scaffold:Pmarinus_7.0:GL476395:350535:368781:1...	[more]

BLAST of SMED30006970 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
LEO1	1.776e-16	27.23	Component of the Paf1 complex; which associates wi...	[more]

BLAST of SMED30006970 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO44827	3.301e-79	55.37	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30006970 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
leo1	1.612e-83	56.64	LEO1 homolog, Paf1/RNA polymerase II complex compo...	[more]

BLAST of SMED30006970 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000009578.1	0.000e+0	99.77	SMESG000009578.1	[more]
SMESG000009578.1	0.000e+0	99.54	SMESG000009578.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30006970 ID=SMED30006970|Name=SMED30006970|organism=Schmidtea mediterranea sexual|type=transcript|length=1489bp
CCAACATTCAATATTAACAATAAAAATACTGACCAATCAGCAAGCATACA
TTATAAAAGACAATATATATATTCATATTACAAACATAACAAATTATTCA
CTAAAACTTTCATCATCTGATATAATTCTAGGCTTTTTGCCCTTCTTCTT
TGGAGGCGGATCTGAGAAATCACTCATAGATTCCTCCTCTTCCTCATCTG
TCGACGACATGTCAATTGTTTTTTTCTTCTTGATTGATGAGCTTGGCCGG
GCTTTTTTCTTGATTTCCGCCAATGAGATCGCGTGAGGATTATTTTCATC
ATCTTTCTGCTGCTCAAGAAAAGCGGCAGTCAGTTCTCGATGGCTGGCGC
GTTCCCGCATCCGCCTGCGCTGGTTTTCACGACGCAAGGATGCCTTCATG
CGTTCCTCCTCGCGCTTGATCATTTCCTGCTGGCGTACATCGGGATTCTC
CCCGGCAAATGGCAACAGCTTGACTTTGGCAGCCTTGTGGGTTTTATCGG
CCAGGGATAGAGTGAGCTTGCGGTGAGTGAATGAATCGGTGGAATGAGGC
CGGAACCCGAGCTTGCTGGTGAAAATTGCTTGTGCTTGCAGGCCGCTGCC
TTCTCGGACGAAAAGGTGATTGTGATCGGCACTGATGTCAGTAGTGTGTA
CGTCGAATATCTCCCGGCCTAAATGTAACGACAGGCTGCCATCGGACCAT
CGGATCATTCGAGCGTTGCTTTCTCGTTTCGGTTGGTCGTTCTCATCAAG
AATCGGTTGGCCTTCACTGTCTTTGGCAATTCGCCATCTGATGGTGTTTT
CCACTTTCAATTTTAGCCTTGTCTTGCCTTCTTCATCCTGAACTTCAGAT
TCGTCCATTTCGTCTTCATAATACATCGGATCGAACGGCCTCGTTTCAAC
AGATAAAAAATTCGGTAACTTGACGAGATGCATTTCCTCTCCCAAGTCTG
CTTTAATTACTGGGAAATCAACTTCAATAGCTCCATCGTCGCCAACTTCA
TCTGGCTCAGGTTCACGTGGAACATCCTCATTGTCAAATTGAGTTTGATA
AGTTTGGTTACCTTCAGGACCCGATTCGTCACTTCCCACAATAATTTCTT
TAATAATGTTAGCTGCTCGTTTATCTTTAACTTTATCTTCATCAGTATCA
CTGCCAAACACATCCTTTTGAGTTAAATTAGCATCATTAGAATCTTCACT
ATCATTGGAATCTAACCCTAAACTTTTAGCAGTATGATTACTTTTTTTTA
TATTTTCATTTTCTGATTCTTCTGCAGAACTTAACTGCTTTATGTTATTT
CGGCTAGGTTTTTCAACGTAACTATCTGATGAATCGTCACTCAATTCGAC
AGTTTGCTGATGAAGTTTTTCTGTCTTAATATTTTGATCATCAGAATCCG
AATTAAACATTAAATGATACAAGAAAATTAAAATGGTTAATTTAAAAATC
AAAATAAATAATATTTATTTTTGATGAGTAATATTTATA

protein sequence of SMED30006970-orf-1

>SMED30006970-orf-1 ID=SMED30006970-orf-1|Name=SMED30006970-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=439bp
MFNSDSDDQNIKTEKLHQQTVELSDDSSDSYVEKPSRNNIKQLSSAEESE
NENIKKSNHTAKSLGLDSNDSEDSNDANLTQKDVFGSDTDEDKVKDKRAA
NIIKEIIVGSDESGPEGNQTYQTQFDNEDVPREPEPDEVGDDGAIEVDFP
VIKADLGEEMHLVKLPNFLSVETRPFDPMYYEDEMDESEVQDEEGKTRLK
LKVENTIRWRIAKDSEGQPILDENDQPKRESNARMIRWSDGSLSLHLGRE
IFDVHTTDISADHNHLFVREGSGLQAQAIFTSKLGFRPHSTDSFTHRKLT
LSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLRRENQRRR
MRERASHRELTAAFLEQQKDDENNPHAISLAEIKKKARPSSSIKKKKTID
MSSTDEEEEESMSDFSDPPPKKKGKKPRIISDDESFSE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000429	neoblast
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR007149	Leo1

Vocabulary: **cellular component**
Term	Definition
GO:0016593	Cdc73/Paf1 complex

Vocabulary: **biological process**
Term	Definition
GO:0016570	histone modification
GO:0006368	transcription elongation from RNA polymerase II promoter

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 325..345
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 331..438
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 113..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..139
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 414..438
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 331..383
None	No IPR available	PANTHER	PTHR23146:SF0	RNA POLYMERASE-ASSOCIATED PROTEIN LEO1	coord: 3..423
IPR007149	Leo1-like protein	PFAM	PF04004	Leo1	coord: 161..330 e-value: 1.8E-50 score: 171.0
IPR007149	Leo1-like protein	PANTHER	PTHR23146	LEO1 PROTEIN	coord: 3..423