CLUMA_CG005324, isoform A

Overview
NameCLUMA_CG005324, isoform A
Smed IDSMED30006949
Length (bp)913
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CLUMA_CG005324, isoform A (SMED30006949) t-SNE clustered cells

Violin plots show distribution of expression levels for CLUMA_CG005324, isoform A (SMED30006949) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CLUMA_CG005324, isoform A (SMED30006949) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CLUMA_CG005324, isoform A (SMED30006949) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006949

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30006949h1SMcG0012458 SmedASXL_004207SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30006949h1SMcG0012458 dd_Smed_v4_888_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30006949h1SMcG0012458 dd_Smed_v4_888_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006949h1SMcG0012458 dd_Smed_v4_888_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006949h1SMcG0012458 Contig8785newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006949h1SMcG0012458 Contig8785uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30006949h1SMcG0012458 dd_888dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Smed sexual biotypeSMED30006949h1SMcG0012458 Contig45602uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006949h1SMcG0012458 Contig45602newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
goblet cellSMED30006949h1SMcG0012458 dd_Smed_v6_888_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG3246 (gene:FBgn0031538 transcript:FBtr0077572)

HSP 1 Score: 55.4546 bits (132), Expect = 2.203e-8
Identity = 43/174 (24.71%), Postives = 80/174 (45.98%), Query Frame = 1 
Query:  112 SENSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENI-LGGGVLSRIVNSATN 630
            S+ S+   +  ++++F+   P G P + +P  DPL + N    V K +   NL + ++   GLS FR   ++ D+  ++    L   ++ + G+Y L     K       G  T+ ++N        L+++   G L   + +I I +    + F+N+ L G V   +VN A N
Sbjct:   45 SQASIAAQVEAMLVHFQQEDPQGLPGVPVP--DPLEVPN----VKKSMGMANLDMKQVKAYGLSKFRIDKMNLDLKEMRFNGGLQLDQMLVKGQYTLSSFFSK-----ANGPFTVVLKNVYAEATAFLAVE-RDGQLATDRIKIDITFSDMTMDFQNLGLVGSVFQSVVNGAPN 206
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG1124 (gene:FBgn0037290 transcript:FBtr0078841)

HSP 1 Score: 53.9138 bits (128), Expect = 3.522e-8
Identity = 38/137 (27.74%), Postives = 68/137 (49.64%), Query Frame = 1 
Query:  148 ILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYL 558
            I + K  + NG PD+ +PS++P +++ L +++ +      + L  +   G SNF+  +L     +   K +++ P+LKI  KY   G+ +  +P  G G+      NFE      +S D+ TG   +  F+   NYL
Sbjct:   43 IEHLKPYLANGIPDIQLPSVEPFKMDTLALQLTEGPQGYKITLKNMEAFGASNFKVTSLKLSEGSEPFKAKIVMPKLKIEAKYTSSGV-LLILPASGGGDFH---ANFE-----GVSADL-TGKTSIHAFK-GANYL 168
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG8997 (gene:FBgn0028920 transcript:FBtr0343671)

HSP 1 Score: 53.9138 bits (128), Expect = 3.637e-8
Identity = 38/170 (22.35%), Postives = 82/170 (48.24%), Query Frame = 1 
Query:  118 NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKF---IPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVN 618
             S++  +  +I   K  +P G   + +P + PLRI++ ++ ++  ++     +    LNGL++F    +  +  T K+  +  F ++ +  +Y L  L  K+   I + G G+    +++  I   +  S+ + +G LKL   E++ +  +     E ILG G ++  +N
Sbjct:   26 QSISSTIVDVIEGIKEQMPCGFTSVGLPPLAPLRIDHQDINIDSSVLKAQGTIDHFRLNGLNDFDIDEMKVNAITSKVTYKFTFRDVNVDTQYDLSVLLKKYGFTINLIGAGHAKFAIKDMVIWGTMKYSLGVISGNLKLKSLEVRTHLGEVDSEIEGILGDGSINEKMN 195
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG8997 (gene:FBgn0028920 transcript:FBtr0080563)

HSP 1 Score: 53.9138 bits (128), Expect = 3.637e-8
Identity = 38/170 (22.35%), Postives = 82/170 (48.24%), Query Frame = 1 
Query:  118 NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKF---IPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVN 618
             S++  +  +I   K  +P G   + +P + PLRI++ ++ ++  ++     +    LNGL++F    +  +  T K+  +  F ++ +  +Y L  L  K+   I + G G+    +++  I   +  S+ + +G LKL   E++ +  +     E ILG G ++  +N
Sbjct:   26 QSISSTIVDVIEGIKEQMPCGFTSVGLPPLAPLRIDHQDINIDSSVLKAQGTIDHFRLNGLNDFDIDEMKVNAITSKVTYKFTFRDVNVDTQYDLSVLLKKYGFTINLIGAGHAKFAIKDMVIWGTMKYSLGVISGNLKLKSLEVRTHLGEVDSEIEGILGDGSINEKMN 195
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG10407 (gene:FBgn0038395 transcript:FBtr0336487)

HSP 1 Score: 47.7506 bits (112), Expect = 4.115e-6
Identity = 33/142 (23.24%), Postives = 69/142 (48.59%), Query Frame = 1 
Query:  175 NGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGA--LKLSQFEIKINYLKGRVYFENILGG 594
             G P+L IP+M+PL +  +++  +   ++L+     + + G+S      L  + + LK  + L FP+L +   Y + G  M  +P+ G G+  + + N  + T +      + GA  LK++  ++K       ++ +N+  G
Sbjct:   65 EGIPELYIPAMEPLVVPQVKMDQDSGAIYLHSVYRNVKVTGISKHTVNELRLEPSKLKFILSLTFPKLHMESDYSIKGKIM-MMPLLGDGHCKVDLVNITMRTELIGQEYKKNGANFLKINTVKVKYELSDVHIHLDNLFNG 205
[Close]
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A267DWM6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g2 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.919e-21
Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 
Query:  106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747
            ++ EN SLN Y++ L+ N K  +PNG PDL+IP ++PL +  +   + + +  + L +  L + GLS F    +  D+NT+   + L  PE+     Y +DG  + F P+YG+G   + V +        + I D+E+  ++L  F I + + + ++Y ENI+GGG L  +VN+  N + + L++  +      +  II   L  +L K+ + D++
Sbjct:   57 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 269
[Close]
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A1I8I9N4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g1 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.333e-20
Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 
Query:  106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747
            ++ EN SLN Y++ L+ N K  +PNG PDL+IP ++PL +  +   + + +  + L +  L + GLS F    +  D+NT+   + L  PE+     Y +DG  + F P+YG+G   + V +        + I D+E+  ++L  F I + + + ++Y ENI+GGG L  +VN+  N + + L++  +      +  II   L  +L K+ + D++
Sbjct:   32 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 244
[Close]
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A1I8IW02 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g3 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.549e-20
Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 
Query:  106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747
            ++ EN SLN Y++ L+ N K  +PNG PDL+IP ++PL +  +   + + +  + L +  L + GLS F    +  D+NT+   + L  PE+     Y +DG  + F P+YG+G   + V +        + I D+E+  ++L  F I + + + ++Y ENI+GGG L  +VN+  N + + L++  +      +  II   L  +L K+ + D++
Sbjct:   32 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 244
[Close]
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A267DI40 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018102g1 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.838e-18
Identity = 58/238 (24.37%), Postives = 130/238 (54.62%), Query Frame = 1 
Query:   55 LSFSLIIVILTILHFKNANSE------NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEV-KVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747
            LS ++   +L  +  +NA +       +++++Y++  + N +  +P+G P+L IP ++PL +  +++ ++ + +  + L +    +NGLS+F          ++ I++ L FP++     Y LDG  + F P+YG G   I + +  I  + ++SI+  T  ++L+  ++ + + + ++Y E I+GGG L  +VN+  N++ + +++  K +    +  I+   + NEL KV + D++
Sbjct:    5 LSLAVACCLLAAVSCRNAETPLSDPDLDTISIYIKATLENLRQAMPHGIPELMIPPLEPLSLPVIDIPRIKEGVADVQLNMRNFVMNGLSSFVVRDAKGHTGSMSIELDLFFPQVAARADYNLDGKILIF-PLYGSGPAAIQINDLSIRFVASVSIEDFT-RVQLNNLQVDLTFSQVQMYLEGIVGGGNLGSVVNTLLNAMGKLIYDRLKGNLLPEVNRILRTVINNELAKVDLRDIL 240
[Close]
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A4Y2ADK2 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_12704_2 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.381e-18
Identity = 57/217 (26.27%), Postives = 111/217 (51.15%), Query Frame = 1 
Query:  100 KNANSENSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVVR 750
            ++  S+  L  YLR+ I NF+  +  G   +++P +DPL + NL + V + L  ++L +  L++  LS F    L  ++  L I + L  PE+ + G+Y +DG  +K  PIYG+G   +   +  I+ +  L    ++  + L + ++   +L   V+ EN LGGG  S ++     ++ R +F   K     ++   +  K+ ++L +  V D+++
Sbjct:   36 QHVESDPDLENYLREAIENFREQMKVGIEAINMPVLDPLELKNLVINVAENLATMDLSIKMLTVRCLSIFNITHLSPNLEELYIAINLTLPEIYVHGQYRVDGKFVKIFPIYGQGYFDLNATDINISGVGKLGFTADSLQMDLLKLDLYWEHLA--VFMENFLGGGSFSEVLQRIVPNVGRDIFNVYKPLMLEKLETCLTDKINSKLNEPIVKDIIK 250
[Close]
BLAST of CLUMA_CG005324, isoform A vs. Planmine SMEST
Match: SMESG000073691.1 (SMESG000073691.1)

HSP 1 Score: 352.443 bits (903), Expect = 8.948e-124
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 1 
Query:  205 MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV 744
            MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV
Sbjct:    1 MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV 180
The following BLAST results are available for this feature:
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG32462.203e-824.71gene:FBgn0031538 transcript:FBtr0077572[more]
CG11243.522e-827.74gene:FBgn0037290 transcript:FBtr0078841[more]
CG89973.637e-822.35gene:FBgn0028920 transcript:FBtr0343671[more]
CG89973.637e-822.35gene:FBgn0028920 transcript:FBtr0080563[more]
CG104074.115e-623.24gene:FBgn0038395 transcript:FBtr0336487[more]
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DWM63.919e-2129.63Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8I9N44.333e-2029.63Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IW024.549e-2029.63Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DI403.838e-1824.37Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A4Y2ADK24.381e-1826.27Uncharacterized protein OS=Araneus ventricosus OX=... [more]
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CLUMA_CG005324, isoform A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000073691.18.948e-124100.00SMESG000073691.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006949 ID=SMED30006949|Name=CLUMA_CG005324, isoform A|organism=Schmidtea mediterranea sexual|type=transcript|length=913bp
TGGCTGTATATAAAAGTCACATGAGAATATCAGCTACCAGTTAAAAAAAT
GAAACTTTCATTCTCTCTGATCATTGTTATATTAACAATTTTGCATTTCA
AAAATGCTAATTCAGAAAATTCCTTGAATTTATATTTAAGAAAATTAATT
TTAAATTTTAAACTTTTGATTCCAAATGGGGATCCTGATCTTGATATTCC
ATCAATGGATCCATTAAGAATTAACAATTTAGAAGTAAAAGTCAATAAAC
CTTTGGTTTGGTTAAATCTTGGACTTCATCGACTTTCTTTGAATGGTTTA
TCAAATTTTCGTACTGCAGCATTAGATGCTGATATTAATACGCTTAAAAT
AAAAGTACGACTAATATTTCCTGAACTTAAAATATCCGGAAAATATGTTC
TCGATGGCCTGGGGATGAAATTTATTCCAATATATGGTAAAGGAAATACG
ACAATAACTGTAAGAAATTTTGAAATCAACACTTTGATTGCGCTTAGTAT
CGATATTGAAACCGGAGCATTGAAACTTTCACAATTCGAAATTAAAATTA
ACTACTTAAAGGGAAGGGTTTATTTTGAAAACATTCTTGGGGGTGGTGTG
CTGAGTCGCATTGTCAACTCGGCCACAAATTCTATTCTTCGGCCATTGTT
TGAGACGAAGAAGATTGATTTTGAAAATAGAATCAGAATAATCATTTTGA
GAAAATTACAAAATGAATTAAAAAAGGTTACCGTGGTAGATGTGGTAAGA
TATCCATTCATTAAGAAATGAAGACCTTGTTTAATTAAAAACAATTTATT
TGTAGACAACGAAAACCAGTTATATTTGGCTAATAATATTGTTAATATTG
AAATAAATTAATTGTTATTAAAAAAAATATATTTTTTAAAATATAATTTC
TAGCTCTTAAAAA
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protein sequence of SMED30006949-orf-1

>SMED30006949-orf-1 ID=SMED30006949-orf-1|Name=SMED30006949-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp
MKLSFSLIIVILTILHFKNANSENSLNLYLRKLILNFKLLIPNGDPDLDI
PSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLK
IKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALS
IDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPL
FETKKIDFENRIRIIILRKLQNELKKVTVVDVVRYPFIKK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0008289lipid binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000067goblet cell
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR038606To_sf
IPR010562Haemolymph_juvenile_hormone-bd
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010562Haemolymph juvenile hormone bindingSMARTSM00700jhbpcoord: 6..231
e-value: 9.4E-11
score: 51.7
IPR010562Haemolymph juvenile hormone bindingPFAMPF06585JHBPcoord: 22..231
e-value: 4.5E-33
score: 114.4
IPR038606Takeout superfamilyGENE3DG3DSA:3.15.10.30coord: 24..233
e-value: 7.8E-24
score: 86.3
NoneNo IPR availablePANTHERPTHR11008UNCHARACTERIZEDcoord: 3..233
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.719