CLUMA_CG005324, isoform A
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006949 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG3246 (gene:FBgn0031538 transcript:FBtr0077572) HSP 1 Score: 55.4546 bits (132), Expect = 2.203e-8 Identity = 43/174 (24.71%), Postives = 80/174 (45.98%), Query Frame = 1 Query: 112 SENSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENI-LGGGVLSRIVNSATN 630 S+ S+ + ++++F+ P G P + +P DPL + N V K + NL + ++ GLS FR ++ D+ ++ L ++ + G+Y L K G T+ ++N L+++ G L + +I I + + F+N+ L G V +VN A N Sbjct: 45 SQASIAAQVEAMLVHFQQEDPQGLPGVPVP--DPLEVPN----VKKSMGMANLDMKQVKAYGLSKFRIDKMNLDLKEMRFNGGLQLDQMLVKGQYTLSSFFSK-----ANGPFTVVLKNVYAEATAFLAVE-RDGQLATDRIKIDITFSDMTMDFQNLGLVGSVFQSVVNGAPN 206
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG1124 (gene:FBgn0037290 transcript:FBtr0078841) HSP 1 Score: 53.9138 bits (128), Expect = 3.522e-8 Identity = 38/137 (27.74%), Postives = 68/137 (49.64%), Query Frame = 1 Query: 148 ILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYL 558 I + K + NG PD+ +PS++P +++ L +++ + + L + G SNF+ +L + K +++ P+LKI KY G+ + +P G G+ NFE +S D+ TG + F+ NYL Sbjct: 43 IEHLKPYLANGIPDIQLPSVEPFKMDTLALQLTEGPQGYKITLKNMEAFGASNFKVTSLKLSEGSEPFKAKIVMPKLKIEAKYTSSGV-LLILPASGGGDFH---ANFE-----GVSADL-TGKTSIHAFK-GANYL 168
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG8997 (gene:FBgn0028920 transcript:FBtr0343671) HSP 1 Score: 53.9138 bits (128), Expect = 3.637e-8 Identity = 38/170 (22.35%), Postives = 82/170 (48.24%), Query Frame = 1 Query: 118 NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKF---IPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVN 618 S++ + +I K +P G + +P + PLRI++ ++ ++ ++ + LNGL++F + + T K+ + F ++ + +Y L L K+ I + G G+ +++ I + S+ + +G LKL E++ + + E ILG G ++ +N Sbjct: 26 QSISSTIVDVIEGIKEQMPCGFTSVGLPPLAPLRIDHQDINIDSSVLKAQGTIDHFRLNGLNDFDIDEMKVNAITSKVTYKFTFRDVNVDTQYDLSVLLKKYGFTINLIGAGHAKFAIKDMVIWGTMKYSLGVISGNLKLKSLEVRTHLGEVDSEIEGILGDGSINEKMN 195
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG8997 (gene:FBgn0028920 transcript:FBtr0080563) HSP 1 Score: 53.9138 bits (128), Expect = 3.637e-8 Identity = 38/170 (22.35%), Postives = 82/170 (48.24%), Query Frame = 1 Query: 118 NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKF---IPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVN 618 S++ + +I K +P G + +P + PLRI++ ++ ++ ++ + LNGL++F + + T K+ + F ++ + +Y L L K+ I + G G+ +++ I + S+ + +G LKL E++ + + E ILG G ++ +N Sbjct: 26 QSISSTIVDVIEGIKEQMPCGFTSVGLPPLAPLRIDHQDINIDSSVLKAQGTIDHFRLNGLNDFDIDEMKVNAITSKVTYKFTFRDVNVDTQYDLSVLLKKYGFTINLIGAGHAKFAIKDMVIWGTMKYSLGVISGNLKLKSLEVRTHLGEVDSEIEGILGDGSINEKMN 195
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Match: CG10407 (gene:FBgn0038395 transcript:FBtr0336487) HSP 1 Score: 47.7506 bits (112), Expect = 4.115e-6 Identity = 33/142 (23.24%), Postives = 69/142 (48.59%), Query Frame = 1 Query: 175 NGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGA--LKLSQFEIKINYLKGRVYFENILGG 594 G P+L IP+M+PL + +++ + ++L+ + + G+S L + + LK + L FP+L + Y + G M +P+ G G+ + + N + T + + GA LK++ ++K ++ +N+ G Sbjct: 65 EGIPELYIPAMEPLVVPQVKMDQDSGAIYLHSVYRNVKVTGISKHTVNELRLEPSKLKFILSLTFPKLHMESDYSIKGKIM-MMPLLGDGHCKVDLVNITMRTELIGQEYKKNGANFLKINTVKVKYELSDVHIHLDNLFNG 205
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A267DWM6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g2 PE=4 SV=1) HSP 1 Score: 101.293 bits (251), Expect = 3.919e-21 Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 Query: 106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747 ++ EN SLN Y++ L+ N K +PNG PDL+IP ++PL + + + + + + L + L + GLS F + D+NT+ + L PE+ Y +DG + F P+YG+G + V + + I D+E+ ++L F I + + + ++Y ENI+GGG L +VN+ N + + L++ + + II L +L K+ + D++ Sbjct: 57 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 269
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A1I8I9N4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g1 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 4.333e-20 Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 Query: 106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747 ++ EN SLN Y++ L+ N K +PNG PDL+IP ++PL + + + + + + L + L + GLS F + D+NT+ + L PE+ Y +DG + F P+YG+G + V + + I D+E+ ++L F I + + + ++Y ENI+GGG L +VN+ N + + L++ + + II L +L K+ + D++ Sbjct: 32 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 244
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A1I8IW02 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021288g3 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 4.549e-20 Identity = 64/216 (29.63%), Postives = 116/216 (53.70%), Query Frame = 1 Query: 106 ANSEN-SLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSI-DIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747 ++ EN SLN Y++ L+ N K +PNG PDL+IP ++PL + + + + + + L + L + GLS F + D+NT+ + L PE+ Y +DG + F P+YG+G + V + + I D+E+ ++L F I + + + ++Y ENI+GGG L +VN+ N + + L++ + + II L +L K+ + D++ Sbjct: 32 SDDENESLNQYIKALLENLKEEMPNGIPDLNIPPLEPLHLPEIYADIEEGMAKVRLHMLDLLIEGLSGFVVREVKGDLNTMSFSLDLFLPEITGQSYYDMDGKVLIF-PLYGRGMAGLKVNDLHFKGTARIVIKDMES--IQLDHFNIHVEFSQIQIYLENIVGGGNLGNVVNTLLNMLGKLLYDKFRDQILPPLNNIIKNALNEQLAKIKLSDLI 244
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A267DI40 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018102g1 PE=4 SV=1) HSP 1 Score: 94.7449 bits (234), Expect = 3.838e-18 Identity = 58/238 (24.37%), Postives = 130/238 (54.62%), Query Frame = 1 Query: 55 LSFSLIIVILTILHFKNANSE------NSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEV-KVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVV 747 LS ++ +L + +NA + +++++Y++ + N + +P+G P+L IP ++PL + +++ ++ + + + L + +NGLS+F ++ I++ L FP++ Y LDG + F P+YG G I + + I + ++SI+ T ++L+ ++ + + + ++Y E I+GGG L +VN+ N++ + +++ K + + I+ + NEL KV + D++ Sbjct: 5 LSLAVACCLLAAVSCRNAETPLSDPDLDTISIYIKATLENLRQAMPHGIPELMIPPLEPLSLPVIDIPRIKEGVADVQLNMRNFVMNGLSSFVVRDAKGHTGSMSIELDLFFPQVAARADYNLDGKILIF-PLYGSGPAAIQINDLSIRFVASVSIEDFT-RVQLNNLQVDLTFSQVQMYLEGIVGGGNLGSVVNTLLNAMGKLIYDRLKGNLLPEVNRILRTVINNELAKVDLRDIL 240
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Match: A0A4Y2ADK2 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_12704_2 PE=4 SV=1) HSP 1 Score: 92.0485 bits (227), Expect = 4.381e-18 Identity = 57/217 (26.27%), Postives = 111/217 (51.15%), Query Frame = 1 Query: 100 KNANSENSLNLYLRKLILNFKLLIPNGDPDLDIPSMDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDVVR 750 ++ S+ L YLR+ I NF+ + G +++P +DPL + NL + V + L ++L + L++ LS F L ++ L I + L PE+ + G+Y +DG +K PIYG+G + + I+ + L ++ + L + ++ +L V+ EN LGGG S ++ ++ R +F K ++ + K+ ++L + V D+++ Sbjct: 36 QHVESDPDLENYLREAIENFREQMKVGIEAINMPVLDPLELKNLVINVAENLATMDLSIKMLTVRCLSIFNITHLSPNLEELYIAINLTLPEIYVHGQYRVDGKFVKIFPIYGQGYFDLNATDINISGVGKLGFTADSLQMDLLKLDLYWEHLA--VFMENFLGGGSFSEVLQRIVPNVGRDIFNVYKPLMLEKLETCLTDKINSKLNEPIVKDIIK 250
BLAST of CLUMA_CG005324, isoform A vs. Planmine SMEST
Match: SMESG000073691.1 (SMESG000073691.1) HSP 1 Score: 352.443 bits (903), Expect = 8.948e-124 Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 1 Query: 205 MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV 744 MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV Sbjct: 1 MDPLRINNLEVKVNKPLVWLNLGLHRLSLNGLSNFRTAALDADINTLKIKVRLIFPELKISGKYVLDGLGMKFIPIYGKGNTTITVRNFEINTLIALSIDIETGALKLSQFEIKINYLKGRVYFENILGGGVLSRIVNSATNSILRPLFETKKIDFENRIRIIILRKLQNELKKVTVVDV 180 The following BLAST results are available for this feature:
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of CLUMA_CG005324, isoform A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006949 ID=SMED30006949|Name=CLUMA_CG005324, isoform A|organism=Schmidtea mediterranea sexual|type=transcript|length=913bpback to top protein sequence of SMED30006949-orf-1 >SMED30006949-orf-1 ID=SMED30006949-orf-1|Name=SMED30006949-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp MKLSFSLIIVILTILHFKNANSENSLNLYLRKLILNFKLLIPNGDPDLDIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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