Tgs-1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006900 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Tgs-1 vs. TrEMBL
Match: A0A2Z4ELZ5 (Tgs-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 136.346 bits (342), Expect = 1.559e-31 Identity = 120/427 (28.10%), Postives = 213/427 (49.88%), Query Frame = 2 Query: 98 NVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNK-RIPSRT--RQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDK-EVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELI--------------------------KGTEPSNKSTNST-IDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQL---TMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFK 1276 N+G + +++ ++ ++ +K +P AF +++Q LN L L D+ FL + ++ R ++N R R R RSMSPSG++ E WE EIA I+++L + LP+NF+ + K + K+++D L ++ K E + L +IG++++ I+ K+G+L P IS +I LK L N I K +S I AVQILLL+I +R L+ K++++F I++ + + E+L ++L S F+ E N ++L KD I L H+F P I++ ++I P LI K T P KS + I +F +SK N + + +++ + +K ++ P + +P + T++N+T++R I K S +K K+ + ++ FK Sbjct: 27 NLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFE-ELNSESIKLCKDMIVLVEHLFLAPL---GILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQES--VILTAFNKINS---PEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFK 444
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000023662.1 (SMESG000023662.1) HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0 Identity = 411/412 (99.76%), Postives = 411/412 (99.76%), Query Frame = 2 Query: 59 MEAQKLTFKSNVINVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNKRIPSRTRQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDKEVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELIKGTEPSNKSTNSTIDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQLTMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFKINSINQ 1294 MEAQKLTFKSNVINVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNKRIPSRTRQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDKEVKRVLDDLKGIKLSERKTEY KRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELIKGTEPSNKSTNSTIDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQLTMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFKINSINQ Sbjct: 1 MEAQKLTFKSNVINVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNKRIPSRTRQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDKEVKRVLDDLKGIKLSERKTEYLKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELIKGTEPSNKSTNSTIDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQLTMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFKINSINQ 412
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000062596.1 (SMESG000062596.1) HSP 1 Score: 366.696 bits (940), Expect = 2.581e-123 Identity = 223/423 (52.72%), Postives = 294/423 (69.50%), Query Frame = 2 Query: 116 SDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNKRIPSRTRQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDKEVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIV---------NIYMIAIVNSN-----QPCELIKGTEPSNKSTNSTIDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQLTMENKTVRRLSIRKKRSFVKTAKQV-----------------LTKTMSPFKINSIN 1291 SD Q +++ +++V+ K +P+AF+K+ QELLN+L+L+LDKSFL EL RSRS++A V+N R SR+RQRS+SPSGSLNK EKSWE+N E+A A IE + ENI+LLP NF+N +DKEVK +LDDLK IK +E++ E KR +L V+GE MK I+ KLGVLLP +ISTEIE LKSL C +NN++KK+KSHILA QILLLNIPEQSR L+QKI++LFN++ H +N N+EQLYELLK+SLFDGENNY+KLQLSKDFI+LSN IF E++ ++ ++ A+ N + +P EL + KS +S+ID YFSHSK DL+L S+ + +P I SGNGKIPQ EN+T++R SIRK+ S + TAKQV LT+T+SPFK +N Sbjct: 17 SDMSKQCNEATIKFVLECKFFYPSAFRKVCQELLNQLNLQLDKSFLTELKIVTRSRSKLAKVINSRTRSRSRQRSISPSGSLNKTEKSWESNIEVACAIIEDIRENINLLPENFLNQPGVDKEVKMLLDDLKVIKTTEKRRERLKRNELTVVGEAMKKILAKLGVLLPTRISTEIELLKSLRCFQNNQLKKRKSHILATQILLLNIPEQSRNLIQKIVELFNMILEHKTVNVNEEQLYELLKISLFDGENNYSKLQLSKDFIHLSNRIFQYSPEVNDVIISKVKSKLNSVGPFAMDNCHPMIIKEPVELNNNNNNNIKSQSSSIDNYFSHSKSIGGKLPDLILKYSTPHSINPIPLIGSGNGKIPQFITENQTIKRRSIRKQNSILSTAKQVNKFISTKHLLIIEIFKALTRTISPFKTKPVN 439
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000062778.1 (SMESG000062778.1) HSP 1 Score: 277.715 bits (709), Expect = 1.588e-88 Identity = 192/448 (42.86%), Postives = 266/448 (59.38%), Query Frame = 2 Query: 35 YKSLSDNSMEAQKLTFKSNVINVGGPPSDRCNQ-------------SSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNKRIPSRTRQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENID--------------------LLPINFMNYSNMDKEVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELIKGTEPSN----------KSTNSTIDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQLTMENKTVRRLSIRKKRSFVKTAKQV 1249 YK L N M+ Q KSN+ SD+ N+ S+ +M+ ++ SK +P FK SQELLNRL L+LDKSFL E RSR +I+ V++ RI SR+RQR S SGSLN+ +KSWENN E AN IE ++ N++ LP NF+N N++KE K++LDDLK K ++ + EY ++ KL VIGE +K I+ K+G L+P +IS+E+E LKSL +++NK K+KSHI+AVQILLLNIPE +RTLL+ II+L+ L H + N EQLY+LL +SLF+GENNYNKLQLSKDFI+LS+ IF CPSEL+ I+ + + +NS QP L EP+ +S +++ S + D L DS+ + + + SG GKI +N T R I+K+ S + TAK+V Sbjct: 3 YKELFCNKMDFQIKNIKSNL---ESDKSDQVNKIFCIILSNIPFSIQSEIVMKSLLESKVFYPLEFKDASQELLNRLKLQLDKSFLIESNGVARSRLKISKVISSRIRSRSRQRVSS-SGSLNRTDKSWENNVEFANVIIEVIMNNLESKLLKFIFQTTFLYFKNYNLALPSNFLNDPNLEKESKKILDDLKVFKTAKERKEYLEKNKLNVIGEALKNILSKIGGLVPLRISSEVELLKSLMNVRDNKACKRKSHIIAVQILLLNIPEMNRTLLKSIIELYKKLMKHKTVKINNEQLYDLLNISLFNGENNYNKLQLSKDFIHLSHRIFQCPSELNDIIGSKLKSKINSKQPALLNNCQEPTTNQIVKQNNNIQSEMVSVEGPIPDSTYDNGNIRDSLFNDSALLSIRSITILASGTGKISLSPKDNTTTLRPLIKKRCSILITAKEV 446
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 125.561 bits (314), Expect = 1.361e-31 Identity = 117/398 (29.40%), Postives = 207/398 (52.01%), Query Frame = 2 Query: 134 SSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNK-RIPSRT--RQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDK-EVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQL---SKDFINL----SNHIFDCPSELHKIVNIYMIAIVNSNQPCELI--KGTEPSNKSTNST-IDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQL---TMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFK 1276 +++ ++ ++ +K +P AF +++Q LN L L LD+ FL + ++ R ++N R R R RSMSPSG++ E WE EIA I+++L + LP+NF+ + K + K+++D L ++ K E + L +IG++++ I+ K+G+L P IS +I LK L N I K +S I AVQILLL+I +R L+ K++++F I++ + + E+L ++L S F+ N+ L + K IN +N I D P + K+ I +P I K T P KS + I +F +SK N + + +++ + +K ++ P + +P + T++N+T++R I K S +K K+ + ++ FK Sbjct: 19 TNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNK----PISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQES--VILTAFNKINS---PEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFK 407
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 125.946 bits (315), Expect = 1.739e-31 Identity = 117/390 (30.00%), Postives = 202/390 (51.79%), Query Frame = 2 Query: 158 VVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNK-RIPSRT--RQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDK-EVKRVLDDLKGIKLSERKTEYFKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQL---SKDFINL----SNHIFDCPSELHKIVNIYMIAIVNSNQPCELI--KGTEPSNKSTNST-IDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQL---TMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFK 1276 ++ +K +P AF +++Q LN L L LD+ FL + ++ R ++N R R R RSMSPSG++ E WE EIA I+++L + LP+NF+ + K + K+++D L ++ K E + L +IG++++ I+ K+G+L P IS +I LK L N I K +S I AVQILLL+I +R L+ K++++F I++ + + E+L ++L S F+ N+ L + K IN +N I D P + K+ I +P I K T P KS + I +F +SK N + + +++ + +K ++ P + +P + T++N+T++R I K S +K K+ + ++ FK Sbjct: 47 LLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNK----PISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQES--VILTAFNKINS---PEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFK 427 The following BLAST results are available for this feature:
BLAST of Tgs-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Tgs-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Tgs-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Tgs-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Tgs-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006900 ID=SMED30006900|Name=Tgs-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1392bpback to top protein sequence of SMED30006900-orf-1 >SMED30006900-orf-1 ID=SMED30006900-orf-1|Name=SMED30006900-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=413bp MEAQKLTFKSNVINVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|