Mediator of RNA polymerase II transcription subunit 23
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006873 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7Note: Hover over icons to view figure legend
Homology
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Match: MED23 (mediator complex subunit 23 [Source:HGNC Symbol;Acc:HGNC:2372]) HSP 1 Score: 152.525 bits (384), Expect = 2.424e-36 Identity = 165/738 (22.36%), Postives = 325/738 (44.04%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 138.272 bits (347), Expect = 5.155e-32 Identity = 128/570 (22.46%), Postives = 251/570 (44.04%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACSEPELDYDKTIE----ICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M +R FL+ + H + NR P GW L L C+ + + + CR + + T+ GK P+ N WR ++ + H ++ +ELM S + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 785 SPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEM-HLRDRAFLKRKLVHAIIGSLKDNR---PQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Match: MED23 (mediator complex subunit 23 [Source:HGNC Symbol;Acc:HGNC:2372]) HSP 1 Score: 152.14 bits (383), Expect = 2.809e-36 Identity = 165/738 (22.36%), Postives = 325/738 (44.04%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPTV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 138.272 bits (347), Expect = 5.211e-32 Identity = 128/570 (22.46%), Postives = 251/570 (44.04%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACSEPELDYDKTIE----ICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M +R FL+ + H + NR P GW L L C+ + + + CR + + T+ GK P+ N WR ++ + H ++ +ELM S + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 785 SPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEM-HLRDRAFLKRKLVHAIIGSLKDNR---PQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Match: MED23 (mediator complex subunit 23 [Source:HGNC Symbol;Acc:HGNC:2372]) HSP 1 Score: 146.747 bits (369), Expect = 6.397e-35 Identity = 168/749 (22.43%), Postives = 326/749 (43.52%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKS--------QNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITL 2336
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K Q C LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPV--LEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPTV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTL 697
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Match: MED23 (mediator complex subunit 23 [Source:HGNC Symbol;Acc:HGNC:2372]) HSP 1 Score: 147.132 bits (370), Expect = 1.001e-34 Identity = 167/746 (22.39%), Postives = 326/746 (43.70%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPK--------SQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K Q C LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPV--LEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 699 HSP 2 Score: 140.198 bits (352), Expect = 1.476e-32 Identity = 129/570 (22.63%), Postives = 251/570 (44.04%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACSEPELDYDKTIE----ICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M +R FL+ + H + NR P GW L L C+ + + + CR + + T+ GK P+ N WR ++ + H ++ +ELM S + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E VK+ + + L+ +L+FI
Sbjct: 791 SPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEM-HLRDRAFLKRKLVHAIIGSLKDNR---PQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVKKIICNLKPALKLRLRFI 1338
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Match: MED23 (mediator complex subunit 23 [Source:HGNC Symbol;Acc:HGNC:2372]) HSP 1 Score: 147.132 bits (370), Expect = 1.021e-34 Identity = 167/746 (22.39%), Postives = 326/746 (43.70%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPK--------SQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K Q C LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPV--LEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 699 HSP 2 Score: 138.272 bits (347), Expect = 5.246e-32 Identity = 128/570 (22.46%), Postives = 251/570 (44.04%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACSEPELDYDKTIE----ICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M +R FL+ + H + NR P GW L L C+ + + + CR + + T+ GK P+ N WR ++ + H ++ +ELM S + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 791 SPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEM-HLRDRAFLKRKLVHAIIGSLKDNR---PQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1338
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Celegans
Match: sur-2 (Mediator of RNA polymerase II transcription subunit 23 [Source:UniProtKB/Swiss-Prot;Acc:Q10669]) HSP 1 Score: 78.1814 bits (191), Expect = 4.463e-14 Identity = 131/640 (20.47%), Postives = 267/640 (41.72%), Query Frame = 3
Query: 2556 IENSNASDSIN-ETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCN-YQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPE-----VMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMT-SFFSVASKLFKFHSYPLYFCYTLIRSQ--IMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTI--EICRELMNKMHTVFLTYGKFGLPY--KNWR-QDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKI-HPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPL------------QADKTGKL--IELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQ 4385
I+ S+ S+++N E I + + L +F +I E++ PA + + + F V S+Y ++E + +V+M L DY IA+ TQ F + I +A +F+ ++ DR L+ L+ + TD ++ I + F P F + + H+ P P + K H+ PE ++ + ++++ + +P YY L+ RL+P+++ + V Q K+ S L+KFH+ P++F Y+++ + +M GP+ + F+ I + H L + + ++ K+ ++ EL+++M + L + P+ K+W+ + S AQ +Y A IELM SP+ +V ++IN+ Q + F +N+ ++ Y +F + ++ E + F + + D+++ S + + N+ + V + W H +++ + + I ++TE T + P + Q + G L ++LV + +A+ + + I++ + DL Y+ KY + G + E++R +LKF + Q
Sbjct: 792 IKASDTSNAVNTEEILSDVRNTARMHTSLTPNQF--VIRYGEDRDPATSHCMLAVLFHFAYNSVDSTYNITSEFYEVMEKKTPKEIVVMG-------------NYLVDYIIADA---KTQDCNEKTF--KNIAKAAALLVFQFQVLRADRLLLSLI-MHPATDEDALICIQIANEFILTPEFQERIRWYHQNVPKKDHFPT---EYIKAIVKYHDAFPEFEACELVRSYDSSSNVHMPTYYGCLIERLLPIMDQYLH--VALEQQGYKLNPQILQSVSMLYKFHAMPIHFMYSVLFTSHGLMSGPDAKSFVLAFATQIEECH--------------LTEAFEKFNHQKSSREQLIMELIDRM-SASLDFILTPPPFVAKDWKIAELSPGAQ--TLYLACIELMASPHSPETLVAAMINVMQMKPHARPF-NIVNLTALLLTALPAAYSNALHDEFVAVFVN-----GETANLKFEEIVFDNYEESLLLNLPNRARTINV---ISQQYWLHCSLSLLNFFSHEYVPRILEHVKTEKDLWYTLRLVMPYLRRYYENWDTAKQMRSQRENFGPLHIVKLVFQKLGSMAE--EGVEIVYEQHLCDLFYNCKYFFAGDFLRNTAITEFAKLPEKMRDRLKFYVSQ 1377
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Celegans
Match: sur-2 (Mediator of RNA polymerase II transcription subunit 23 [Source:UniProtKB/Swiss-Prot;Acc:Q10669]) HSP 1 Score: 78.1814 bits (191), Expect = 4.693e-14 Identity = 131/640 (20.47%), Postives = 267/640 (41.72%), Query Frame = 3
Query: 2556 IENSNASDSIN-ETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCN-YQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPE-----VMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMT-SFFSVASKLFKFHSYPLYFCYTLIRSQ--IMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTI--EICRELMNKMHTVFLTYGKFGLPY--KNWR-QDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKI-HPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPL------------QADKTGKL--IELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQ 4385
I+ S+ S+++N E I + + L +F +I E++ PA + + + F V S+Y ++E + +V+M L DY IA+ TQ F + I +A +F+ ++ DR L+ L+ + TD ++ I + F P F + + H+ P P + K H+ PE ++ + ++++ + +P YY L+ RL+P+++ + V Q K+ S L+KFH+ P++F Y+++ + +M GP+ + F+ I + H L + + ++ K+ ++ EL+++M + L + P+ K+W+ + S AQ +Y A IELM SP+ +V ++IN+ Q + F +N+ ++ Y +F + ++ E + F + + D+++ S + + N+ + V + W H +++ + + I ++TE T + P + Q + G L ++LV + +A+ + + I++ + DL Y+ KY + G + E++R +LKF + Q
Sbjct: 790 IKASDTSNAVNTEEILSDVRNTARMHTSLTPNQF--VIRYGEDRDPATSHCMLAVLFHFAYNSVDSTYNITSEFYEVMEKKTPKEIVVMG-------------NYLVDYIIADA---KTQDCNEKTF--KNIAKAAALLVFQFQVLRADRLLLSLI-MHPATDEDALICIQIANEFILTPEFQERIRWYHQNVPKKDHFPT---EYIKAIVKYHDAFPEFEACELVRSYDSSSNVHMPTYYGCLIERLLPIMDQYLH--VALEQQGYKLNPQILQSVSMLYKFHAMPIHFMYSVLFTSHGLMSGPDAKSFVLAFATQIEECH--------------LTEAFEKFNHQKSSREQLIMELIDRM-SASLDFILTPPPFVAKDWKIAELSPGAQ--TLYLACIELMASPHSPETLVAAMINVMQMKPHARPF-NIVNLTALLLTALPAAYSNALHDEFVAVFVN-----GETANLKFEEIVFDNYEESLLLNLPNRARTINV---ISQQYWLHCSLSLLNFFSHEYVPRILEHVKTEKDLWYTLRLVMPYLRRYYENWDTAKQMRSQRENFGPLHIVKLVFQKLGSMAE--EGVEIVYEQHLCDLFYNCKYFFAGDFLRNTAITEFAKLPEKMRDRLKFYVSQ 1375
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Fly
Match: MED23 (gene:FBgn0034795 transcript:FBtr0071903) HSP 1 Score: 136.732 bits (343), Expect = 7.499e-32 Identity = 133/596 (22.32%), Postives = 251/596 (42.11%), Query Frame = 3
Query: 2640 NKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHL--DNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVA--SKLFKFHSYPLYFCYTLIR--SQIMWG-PENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSE----PELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRG---GPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK---SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
NKQ+ + + + + N F R ++ F C+ ++I E N+S V + ++ R LR CDY +A + S ++ K ++ + I+K N+++ DR ++ L R + + +V F +I + K N + E C + NP H NW H + H+K PE + +QI LP Y++++ LR +PVL++++ + + F + S L+KFH P+ + Y + +I+ P ++ L + E + N + L + PEL Y + + R L + + GK +WR ++ +A H +Y +EL+ +P +V ++I++ YIN G ++A + Y + ++ P + + F F + + V L + HS++ H ++ Y++E ++P + TE Q L CH+ P + ++ +K + + LY L V K P+ + D I D +YH+KYI+VG+ + + + L+ +L+FI
Sbjct: 753 NKQQLKKAVEEEYRTWTSMTNENDIIAHFLRP-TTNPLFLCLLFKIIWETENISPVAYKILEGISARALSTHLRKFCDYLVAEVASSSDGRDFIH-----KCVDTINNMIWKFNVVTIDRVVLCLALRTHEGNEAQVCFLIIQLLLLKASEL---RNRVQEFCKD--NNPDHWKQSNWQDKHLSFHQKYPEKFALDESASQIPLPVYFSNVCLRFLPVLDVVVHRFIELTITNVHQILGFILDHLSILYKFHDRPITYLYNTLHYYERILRDRPALKKKLVGAITSAFSEIRPPNWSVSEPYKVYLQSQDSLWTPELSY--YMSLIRRLADTIS------GKNVFYSTDWRFNEFPNAPTHALYVTCVELLGLPVAPPLVASNLIDVIVSGYAVIPQKDIHSYINAVGIVLAALPEPYWSGIYDRLQDMLNTPNMLNWTYRFNAFELF-----NFKTVREAMLEKTYAVVLAVAHSVFHHMGAFKLAAMTRYLKEKLKPCVRTEQQLLYLCHVFGPFLQRIELEKPNAVAGIAVLLYEILEIVDKHHGPKPLQYMDQICDFLYHIKYIHVGNIIKNESEAIIKRLRPLLQMRLRFI 1324 HSP 2 Score: 127.102 bits (318), Expect = 6.958e-29 Identity = 117/519 (22.54%), Postives = 223/519 (42.97%), Query Frame = 3
Query: 123 KPTTDDKWINTAISD---SWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKL--------LIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPAN-------------HWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNE 1607
KP T+ + +D ++ ++++++ L+ + + ++ VL L ++ ++ + + + ++ E L+ E++ F+ + I ++ +DYK RE+ +K+ FP +++ L + ++R + R N L+PAYF +E+M+ F H L+ + F+ FR A++ ++P HF Y H NS ++LD N+L + KG++ Y + Q +L Q S ++ + L K Q +Y+ LE LI M EA D A W W+ HL ++ F+ + F I L+ K + K R + W ++ SG I KN + +F+P + FD+LYP + DI + ++++ H P+ +W+HL++K++ N+ R LP AL+ + + ++ N+ G + +I+I NA+ST E
Sbjct: 29 KPNTEQERATRFANDLVTAFGNVAQENREQVLRLYLLRAAGASGYHIKVLMAALVKLVDAHVITARMLCDKVLMCEKLDFEHRTFWIESFRLIKRVIVQVDYKGVREIMKVCRDKAQWFPLNVNVTYMPQLLAVEDILRFIFDRNNCLLPAYFIANEIMRPF-------PYHWKLNRLMTDFVEEFRTTAQMVSIIGHASMLPIVEHFGYA----DHMMNS-WRLDHNTLKFN--FKGSLPYEPELLEEQKPLLRYVLEQPYSREMVSQMLNLQKHQKQRYNALEEQLVNLIVQAMEMTEANDATAGSGFNSSDEQITPYEWMWL-----HLSSQLIYFVLFQFVSFMHIVLALHEKLSKLELRKGRDQLMWILLQFISGSIQKNPITNFLPVFRLFDLLYPELEPLKLPDINK----------------SSMVRH-----------MAPICVWIHLMKKARVENMNITRPLPIALKNHYDFLQHLVT---ANTMMNMTLGNDF-RIILICNAYSTNQE 497
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Zebrafish
Match: med23 (mediator complex subunit 23 [Source:NCBI gene;Acc:445483]) HSP 1 Score: 146.747 bits (369), Expect = 8.585e-35 Identity = 160/742 (21.56%), Postives = 321/742 (43.26%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W + ++ ++++++ I ++ + L+ L++ +E ++ + + L++S+SL E + + + I ++ +DYK R++ ++K PN +SS + L + +V+ +L R L+PAYFA+ E+ K CPE H LL + I+ F+ SFR A++ R PVV+ + + + +KLD +L +P +G + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G S + + N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LP K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRK-LCPEGALS--HWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFP--LRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGT-----------------NCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPNV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 125.561 bits (314), Expect = 2.746e-28 Identity = 122/572 (21.33%), Postives = 246/572 (43.01%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS----EPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKC-QEFVRMFFT--DFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + + +VH I+ + NR TP GW L L C E + C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I ++ +++ F DF S++H L L H++W H+++ Q++ ++ E ++P ++TE Q L H+ P + Q ++T ++E+ Y L V + S + + D I D +YH+KY++ G V + V+ + +R +L+FI
Sbjct: 785 SPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQ----SDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLK-DNR--TP-GWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMH-------CSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Zebrafish
Match: med23 (mediator complex subunit 23 [Source:NCBI gene;Acc:445483]) HSP 1 Score: 146.747 bits (369), Expect = 9.575e-35 Identity = 160/742 (21.56%), Postives = 321/742 (43.26%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W + ++ ++++++ I ++ + L+ L++ +E ++ + + L++S+SL E + + + I ++ +DYK R++ ++K PN +SS + L + +V+ +L R L+PAYFA+ E+ K CPE H LL + I+ F+ SFR A++ R PVV+ + + + +KLD +L +P +G + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G S + + N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LP K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 54 AFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRK-LCPEGALS--HWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFP--LRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGT-----------------NCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPNV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 704 HSP 2 Score: 125.561 bits (314), Expect = 2.808e-28 Identity = 122/572 (21.33%), Postives = 246/572 (43.01%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS----EPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKC-QEFVRMFFT--DFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + + +VH I+ + NR TP GW L L C E + C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I ++ +++ F DF S++H L L H++W H+++ Q++ ++ E ++P ++TE Q L H+ P + Q ++T ++E+ Y L V + S + + D I D +YH+KY++ G V + V+ + +R +L+FI
Sbjct: 796 SPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQ----SDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLK-DNR--TP-GWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMH-------CSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFI 1343
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Xenopus
Match: slc36a4 (solute carrier family 36 (proton/amino acid symporter), member 4 [Source:Xenbase;Acc:XB-GENE-994062]) HSP 1 Score: 140.969 bits (354), Expect = 7.864e-33 Identity = 123/504 (24.40%), Postives = 229/504 (45.44%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWG 1649
A W V+ ++ ++++++ I ++ + L+ L++ +E + + + +L+NS+SL E + + + I ++ +DYK R++ +EK P+ +SS + L + +V+ +L R L+PAY A+ E++K + PE K PH LL ++ F+ SFR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P +F + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G
Sbjct: 34 AFRQYWSVLPQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWILTFKLIRKIIGGVDYKGVRDLLKVILEKIQTVPDTVSSAVVQQLLAAREVVAYILERNACLLPAYLAVTEVLKLY-PEGK--LPHWLLGDLVSDFVDSFRPTARISSICGRCSLLPVVNNSGAICNSWKLDPLTLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDITK-----------------------PQSTHSFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLQMTDYKIALLCNAYSTNSECFTLPMGVLVETIYG 492 HSP 2 Score: 115.546 bits (288), Expect = 3.935e-25 Identity = 123/590 (20.85%), Postives = 245/590 (41.53%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDY------------DKT--IEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN*IFPHRPCLN 4424
S F C+ +++LE ++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + + +VH I+ + NR P GW L S+ L + D T ++ L++ + L + +F P H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + I+ P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y L V + S + D I D +YH+KY++ G + + V++ + L+ +L+ I + P P +N
Sbjct: 776 SPPLFLCLLWKMLLETDQINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEMLNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVNPPVQIQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMQLRDRTNLKRKLVHAIIGSLK-DNR---PQGWCL---SDTYLKFGMNPRDDNPWIPDDTYYCKLIGRLVDNILYCELMFNEFPNP------------AAHALHVTCVELMALAVPGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSILNSPSLTSETEWVGYPFQLF-----DFTACHKAYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLPEVLMPIVKTEYQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYEMLLNVDQCSAHLNFMDPICDFLYHMKYMFTGDSIKDQVEKIICNLRPALKLRLRHITHISTESAAPPPPPMN 1331
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Xenopus
Match: slc36a4 (solute carrier family 36 (proton/amino acid symporter), member 4 [Source:Xenbase;Acc:XB-GENE-994062]) HSP 1 Score: 140.584 bits (353), Expect = 8.580e-33 Identity = 123/504 (24.40%), Postives = 229/504 (45.44%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWG 1649
A W V+ ++ ++++++ I ++ + L+ L++ +E + + + +L+NS+SL E + + + I ++ +DYK R++ +EK P+ +SS + L + +V+ +L R L+PAY A+ E++K + PE K PH LL ++ F+ SFR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P +F + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G
Sbjct: 43 AFRQYWSVLPQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWILTFKLIRKIIGGVDYKGVRDLLKVILEKIQTVPDTVSSAVVQQLLAAREVVAYILERNACLLPAYLAVTEVLKLY-PEGK--LPHWLLGDLVSDFVDSFRPTARISSICGRCSLLPVVNNSGAICNSWKLDPLTLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDITK-----------------------PQSTHSFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLQMTDYKIALLCNAYSTNSECFTLPMGVLVETIYG 501 HSP 2 Score: 125.561 bits (314), Expect = 4.138e-28 Identity = 127/590 (21.53%), Postives = 250/590 (42.37%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDY------------DKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN*IFPHRPCLN 4424
S F C+ +++LE ++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + + +VH I+ + NR P GW L S+ L + D T C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + I+ P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y L V + S + D I D +YH+KY++ G + + V++ + L+ +L+ I + P P +N
Sbjct: 785 SPPLFLCLLWKMLLETDQINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEMLNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVNPPVQIQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMQLRDRTNLKRKLVHAIIGSLK-DNR---PQGWCL---SDTYLKFGMNPRDDNPWIPDDTY-YCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSILNSPSLTSETEWVGYPFQLF-----DFTACHKAYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLPEVLMPIVKTEYQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYEMLLNVDQCSAHLNFMDPICDFLYHMKYMFTGDSIKDQVEKIICNLRPALKLRLRHITHISTESAAPPPPPMN 1348
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Match: Med23 (mediator complex subunit 23 [Source:MGI Symbol;Acc:MGI:1917458]) HSP 1 Score: 152.525 bits (384), Expect = 1.714e-36 Identity = 165/744 (22.18%), Postives = 325/744 (43.68%), Query Frame = 3
Query: 153 TAISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS+SL E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I +P LP + +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ HR +++ P + L + +V Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 45 AAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRVP---LPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSHRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 696 HSP 2 Score: 127.487 bits (319), Expect = 8.007e-29 Identity = 123/570 (21.58%), Postives = 244/570 (42.81%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL--------LACSE-----PELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ +++LE ++S + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + +VH I+ + NR P GW L + E PE Y C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + I++ I + + +D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 792 FLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLK-DNR---PQGWCLSDTYLKHAMNAREDNPWVPEDSY-----YCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDR----IVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1335
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Match: Med23 (mediator complex subunit 23 [Source:MGI Symbol;Acc:MGI:1917458]) HSP 1 Score: 147.132 bits (370), Expect = 7.366e-35 Identity = 167/752 (22.21%), Postives = 326/752 (43.35%), Query Frame = 3
Query: 153 TAISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPK--------SQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS+SL E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K Q C LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I +P LP + +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ HR +++ P + L + +V Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 45 AAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPV--LEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRVP---LPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSHRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 702 HSP 2 Score: 127.487 bits (319), Expect = 8.100e-29 Identity = 123/570 (21.58%), Postives = 244/570 (42.81%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL--------LACSE-----PELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ +++LE ++S + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + +VH I+ + NR P GW L + E PE Y C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + I++ I + + +D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 798 FLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLK-DNR---PQGWCLSDTYLKHAMNAREDNPWVPEDSY-----YCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDR----IVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1341
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Match: Med23 (mediator complex subunit 23 [Source:MGI Symbol;Acc:MGI:1917458]) HSP 1 Score: 125.946 bits (315), Expect = 1.523e-28 Identity = 116/560 (20.71%), Postives = 242/560 (43.21%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHI---LQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ +++LE ++S + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + +VH I L++++ C + T + + + + +L+ ++ V GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y I++ I + + +D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 432 FLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQGWCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRL--VDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIV----LHDRIVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 975
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Match: Med23 (mediator complex subunit 23 [Source:MGI Symbol;Acc:MGI:1917458]) HSP 1 Score: 83.1889 bits (204), Expect = 5.090e-17 Identity = 69/253 (27.27%), Postives = 115/253 (45.45%), Query Frame = 3
Query: 534 LLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIRE 1292
+L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y + R +++ + DL Y E +++ K+ + QL++ +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI +
Sbjct: 3 ILERNACLLPAYFAVTEIRKLY-PEGK--LPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKHKQRCP-------VLEDQLVDLVVYAMERSETEE-KFD--DGGTSQLLW-----------------------QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK 217
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Match: Med23 (mediator complex subunit 23 [Source:MGI Symbol;Acc:MGI:1917458]) HSP 1 Score: 79.337 bits (194), Expect = 4.359e-16 Identity = 47/180 (26.11%), Postives = 94/180 (52.22%), Query Frame = 3
Query: 153 TAISDSWRVISKDDKPAALKFLIQVIVDR-NINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKL 689
A W +S++ ++++++ I + + + L+ L++ +E + + + +L+NS+SL E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++
Sbjct: 18 AAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARI 194
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Match: sp|Q16HH9|MED23_AEDAE (Mediator of RNA polymerase II transcription subunit 23 OS=Aedes aegypti OX=7159 GN=MED23 PE=3 SV=1) HSP 1 Score: 157.532 bits (397), Expect = 3.003e-37 Identity = 133/555 (23.96%), Postives = 246/555 (44.32%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHL--DNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALK-MTSFFSVASKLFKFHSYPLYFCYTLI---RSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGP-----KFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPIL---HSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ ++I+E +S V + + R LR LCDY + V +S VN K ++ + I+K NII+ DR ++ L R + + +V FC+I + K F N L E ++ +P H +NW++ H A H+K PE + + LP Y+ ++ LR +PVL++ I + P K + L+KFH P+ + Y + + P+ ++ L V L++ + N T ++ + +++ + L+ +M F P +WR ++ + H +Y +EL+ PQ V S+I++ +G P +IN G +MA + Y + ++I C + + ++ + + V L + S+ H+ + QI+ +I+E +P I+TE Q + CH+ P + L A+++ + ++ LY L QV K+ P L + D I DL+YH+KY+++G + ++ + L+ +L+FI
Sbjct: 808 FLCLLFKMIVETDTISPVAYKILERIGARALSAHLRKLCDYLVFE-VSNSGDGAHVN-----KCVDTINDMIWKYNIITIDRLVLCLSLRTLEGNEAQVSFCIIQLLLLKTSEF---RNRLQEFV--SINSPEHWKQNNWHERHLAFHQKFPEKFTPDESVSHPSLPVYFGNVCLRFLPVLDITIHRYLEVPAAMSKTLDVLLDHLGPLYKFHDRPITYLYNTLHYYERMLRDRPQLKKRLVGTVIGSLKDVRPDNWAVTEQYQSYMSKKDDAVNWMPDLNYYIYLVRRMQDTIDGTNIF--PGTDWRFNEFPNPPAHALYVTCVELLGLPVGPQGVANSLIDVV--VKGYPVIPSQSVHNWINTIGLIMAALPESYWGVIYERLREVI-----SCPQMTDWLHRQSPFELFNFKIVREAMLEKNYVVILAIAQSILHHSGIGQISTVTEHIKENFKPIIKTEYQLIYLCHLVGPFLNRLCAERSRAVSDITLILYELLEQVDKAQPSLALKYMDPICDLLYHIKYMFIGDMMKSDLEAIIRRLRPALQMRLRFI 1342 HSP 2 Score: 115.546 bits (288), Expect = 2.228e-24 Identity = 105/449 (23.39%), Postives = 193/449 (42.98%), Query Frame = 3
Query: 180 ISKDDKPAALK-FLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSI---FKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPANH-----------WHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQ 1481
+S++ K AL+ LI V + N + ++L + + I + + +++SE L +N+ F+ C+ + ++ +DYK RE+ EK+ FP+ ISS + +VI + +R L+PAYF ++E+ K + H + I F+ F A++L P+V + +++ +KLD +L +G + Y + + Q+ +L Q S D+ + L K+Q K LE QL++ VM +E + H W W+ HL ++ F+ F I L+ K A + + R ++ W ++ SG I +N + +F+P LK +D LYP + D L + P +W+HL+++++S N+ R++P AL+
Sbjct: 52 MSQEVKENALRSCLIHVAGINHRNKVRKHMQVLKDLVGKGIIPARLLCEQIMSSEKLVYQNQSFWIECFQVVRRVIGGVDYKGVREIMKCCKEKALSFPSGISSSILPQMLELTEVIEHIFNRNACLLPAYFIINEIQKA-----DYQDTHWRIANLIANFIEEFVIVAQMLSIIGHSSMLPIVEHSSYADNLINPWKLDPTTLKL--ALRGNLPYEPELLQPQIKLLRFVLEQPYSRDMVCSMLNLQKTQKQKCMALEE---QLVWLVMCAMECSEKEPVHTVDGEISSHTQWVWL-----HLSSQLIYFVLFQFATFQNIVNTLHDKLAVSNLRRGRDHLMWVLLQYISGSIQRNSITNFLPILKLYDSLYPEKEPLPVPDYNNPLCTH---------------------------QMAPTCIWIHLLKRAQSEHYNINRSIPTALK 458
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Match: sp|Q9ULK4|MED23_HUMAN (Mediator of RNA polymerase II transcription subunit 23 OS=Homo sapiens OX=9606 GN=MED23 PE=1 SV=2) HSP 1 Score: 152.14 bits (383), Expect = 1.349e-35 Identity = 165/738 (22.36%), Postives = 325/738 (44.04%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I IP LP N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +V Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRIP---LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPTV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 138.272 bits (347), Expect = 2.503e-31 Identity = 128/570 (22.46%), Postives = 251/570 (44.04%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACSEPELDYDKTIE----ICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M +R FL+ + H + NR P GW L L C+ + + + CR + + T+ GK P+ N WR ++ + H ++ +ELM S + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 785 SPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEM-HLRDRAFLKRKLVHAIIGSLKDNR---PQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Match: sp|Q80YQ2|MED23_MOUSE (Mediator of RNA polymerase II transcription subunit 23 OS=Mus musculus OX=10090 GN=Med23 PE=1 SV=2) HSP 1 Score: 152.14 bits (383), Expect = 1.430e-35 Identity = 165/744 (22.18%), Postives = 325/744 (43.68%), Query Frame = 3
Query: 153 TAISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS+SL E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I +P LP + +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ HR +++ P + L + +V Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 42 AAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRVP---LPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSHRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 127.102 bits (318), Expect = 5.985e-28 Identity = 123/570 (21.58%), Postives = 244/570 (42.81%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL--------LACSE-----PELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ +++LE ++S + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + +VH I+ + NR P GW L + E PE Y C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + I++ I + + +D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 789 FLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLK-DNR---PQGWCLSDTYLKHAMNAREDNPWVPEDSY-----YCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDR----IVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Match: sp|Q5EB59|MED23_RAT (Mediator of RNA polymerase II transcription subunit 23 OS=Rattus norvegicus OX=10116 GN=Med23 PE=2 SV=2) HSP 1 Score: 149.443 bits (376), Expect = 9.934e-35 Identity = 164/744 (22.04%), Postives = 325/744 (43.68%), Query Frame = 3
Query: 153 TAISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILP-YNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W +S++ ++++++ I ++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +VI +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + I +P LP + +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ HR +++ P + L + +V Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 42 AAFRQFWNGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLY-PEG--KLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYG---------------NGIMRVP---LPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSHRM-HHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 122.479 bits (306), Expect = 1.808e-26 Identity = 127/571 (22.24%), Postives = 244/571 (42.73%), Query Frame = 3
Query: 2754 FYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL--------LACSE-----PELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYR-QYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F C+ +++LE ++S + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + M + +VH I+ + NR P GW L + E PE Y C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y S I + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y+ L V + S + + D I D +YH+KY++ G V E V++ + + L+ +L+FI
Sbjct: 789 FLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLK-DNR---PQGWCLSDTYLKHAMNAREDNPWVPEDSY-----YCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSSSLTSETEWVGYPFRLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Match: sp|Q5RIW8|MED23_DANRE (Mediator of RNA polymerase II transcription subunit 23 OS=Danio rerio OX=7955 GN=med23 PE=2 SV=2) HSP 1 Score: 146.747 bits (369), Expect = 6.481e-34 Identity = 160/742 (21.56%), Postives = 321/742 (43.26%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W + ++ ++++++ I ++ + L+ L++ +E ++ + + L++S+SL E + + + I ++ +DYK R++ ++K PN +SS + L + +V+ +L R L+PAYFA+ E+ K CPE H LL + I+ F+ SFR A++ R PVV+ + + + +KLD +L +P +G + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G S + + N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LP K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 43 AFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRK-LCPEGALS--HWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFP--LRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGT-----------------NCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPNV---------FKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 693 HSP 2 Score: 125.561 bits (314), Expect = 2.073e-27 Identity = 122/572 (21.33%), Postives = 246/572 (43.01%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS----EPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKC-QEFVRMFFT--DFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + + +VH I+ + NR TP GW L L C E + C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I ++ +++ F DF S++H L L H++W H+++ Q++ ++ E ++P ++TE Q L H+ P + Q ++T ++E+ Y L V + S + + D I D +YH+KY++ G V + V+ + +R +L+FI
Sbjct: 785 SPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQ----SDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLK-DNR--TP-GWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMH-------CSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFI 1332
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Match: R7TT05 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_178592 PE=4 SV=1) HSP 1 Score: 297.36 bits (760), Expect = 1.057e-78 Identity = 321/1418 (22.64%), Postives = 593/1418 (41.82%), Query Frame = 3
Query: 270 ILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDN-----------PANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVL-AGMSWHELIMILNAFSTKNE-TAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLL---MISIFSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQ-------RLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSIXXXXXXXXXXXALLP------VRFNVLQIKDIPEXXXXXXXXXXXXXXXIENSNASDSINE-TICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVN-FLHEQCPNTLGNPCHLDNWYKIHFALHEKLPE------VMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLI---RSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQE---QRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDP---IVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQ--LCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHS-DTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
+L +E NI + + +L+NS+ L+ ++ + + I ++ +DYK R++ +EK+ P++ S + + +V+ ++ RK L+P+Y A++E+ K + ED+ PH L + F+ SFR A+L+ R PVV+ + + + ++KL+ +L + G + YS +S Q +L Q S D+ + L K + S LE QL+ V+L +E +N +N W +HL ++ F+ F + L +K + R K R ++ W ++ SG I KN LEDF+P ++ +D+LYP + DI + +F T +W+HL +K+++ L R P AL E + +C +V M+ + + ++ NA+ST + A+ +G + E ++G + T +LP NI +P ++ +++VH + H + +I + N ++P ++E++ RL+++ E++ + FI++ +LP+ K + HT+L++ +R +++ P I L + SVP Q+ ++ L+L + GL V P++ R L+ +SEE+N+ +V+TL ++ + S N+ + + T QQ + + A P + N +Q +D S ++ SD IN ++ G+ G P L +L W+ LV F A +++ D L R+ LR DY + + ++N K +E+L I+K NIIS DR ++ L RNF+ + +V F +I + + P F + VN F+ E P+ W++ H A H PE S + LP Y+ +L LR +PV +++I L+ P + L+KFH PL + Y + ++ P ++ L + L++ + +N C T + E +++ + L+ ++ + P +WR ++ + H ++A IELM +V +I++ + G K ++N G ++ Y + ++ + P I+ F + F H + I + L + HS+W HA++ Q++ +++E +RP +++E QFL CH+ P + ++T L+EL ELY L V KS+ LH D I D +YH+KY++VG GV V++ + +R +L+FI
Sbjct: 1 MLETLVENNIVPARGVCESLLNSKELSHKSPDKWNSTFRLIRKIIGGVDYKGCRDLLRVMLEKTHSIPSEEDSSVMQLMDEVHRVVSYVIDRKTCLLPSYLAVNEITKLYG-EDR-AVPHWKLGILLTDFVQSFRPAAQLVSISGRASLLPVVAHSSAVSHVWKLNSCNLRFQ--LNGPLPYSKELSEPQTALLRYVLEQPYSRDMVCNMLSLNKQVKQRCSVLEE---QLVDLVVLAMEGSENNNTDNSMSDENDSNQLLW-----QHLSSQLIFFVLFQFASFPHMVNSLYSKLSKRELRKGRDHLMWILLQFISGSIQKNPLEDFLPIMRLYDLLYPEKESLPLPDISKPAATFKMAVT---------------------------CIWIHLNKKAQNDKVKLQRPFPHALHSHIEFLK---------NCMSVKNLPMTDYRIALLCNAYSTNTDYYALPMGILVESIYG------------------NNQKTSMLPGNITASAPTQPLPMSMLDSLTVHAKMSLIHSIVTRVIRLAQNKSNAVALAPALVETYSRLLVYIEIESLGIKGFISQ---------LLPS---------VFKSHAWGILHTLLEMFSYRL-HHIQPHYRIQLLSHLHSLASVPQTNQNQLHICVESTALRL----------IMGLGSGEVQPQLSRVLNEPKTLTLLSSESEELNRALVLTLA---RSLHVTGCDSSGNWCRDILTTIQQH--TPHSWASHTLAYFPQVISDFYKQNPIQRED---KNTLKRSVDTEYRKWKSMASESDLINHFSMQGSQGMSPLFLCVL-----------------------------WKSLVEDKRFAPTAYKVL--------------DRLGPRLLSAHLRTFADYLVYEFAT-AGAGQQIN-----KCVESLNDLIWKCNIISVDRLVLCLALRNFEENESQVCFFIIQVVLVRSPEFRNRVNEFVKENAPDHWMQ----TGWHEKHMAFHRTYPERFYYDGAQDISQASQHQYLPVYFGNLCLRFLPVFDILIHRLLEAPPVPKFWDLLLDQLGGLYKFHDRPLTYLYNTLHYYERKLREHPSFKKKLVGTIIGALKDIKSANWCLTEDYRSFMKQPPEESNWNPDQDYFNRLIGRIVSTIAGDNPPPFPTCDWRFNEFPNPAAHALHATCIELMALPVDSHVVGAELIDIVLKGPVHIGRKKVFPWMNAIGMVLTALPDGYWIVIHERVAETLQLPHLGIMSSDPFHMLNFN------------HSHSVIAEFSCSYVLAMCHSVWQHASIGQLSILPAFLKEKLRPLVKSEAQFLFVCHLVGPFLQRFHTERTRCLLELTVELYEMLENVDKSVDHLHHMDPITDFLYHIKYMWVGDGVKHEVEKIIRNLRPAMRMRLRFI 1250
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Match: A0A4S2MAM6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001440 PE=4 SV=1) HSP 1 Score: 286.96 bits (733), Expect = 1.477e-74 Identity = 249/821 (30.33%), Postives = 380/821 (46.29%), Query Frame = 3
Query: 171 WRVISKDD-KPAALKFLIQVIVDRNINNLHVLFKIL---SVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEER---RSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDL-AGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPS------------------NDKTWSDDIREA---LKSFGDNTTR------RGNLETNIMEHPPNGMLAFFLDTGPLVL----------------------------------WMHL-----------IRKSKSLPTNLPRNLPPALQPMCEVVTQE--------ISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLW-GIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIFSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTN----GQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSII---------------------------LNISVPN---ELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVP 2264
W+ I++ K AL L + N N+ V+ +IL + IE+ ++ +++A +LV S+ L VE+ FFR + + L+ LDYK+NREVFNKSIEKS F D +++R R + +++I+ LLSR+ LMPAYFAMHEL++ F P + PHK + + +FM SF +PA+LL P+ IT T F+LD +L V KGA+ Y D ++ +Q +L Q RST+L AG L + GLE A I +M E+ D+ W+ EHL+ V+ F+ S +I FSEI KLN K R W KS Y+ WFV+N TSGFI KNML DFVPCL+ FDIL+P D+ D +A L DN+ RG E N+ + + + D+G V MHL +RKS++ +LPR +PPAL+P ++ +S VLA ++W +L +ILNA+ST NE + LI + + W + + S+ + + + LPY + G+ + + +I MS+H+R L HLL+ + + +P ++E+ CR++ RE++ N + + S L K +LP N N S P S L T+LD L +R P++ + +++ L S N E+P+++ + +++ I++LAR Q D V+YGLS P
Sbjct: 56 WKSIAQQKIKIHALDHLFSKLA--NNNHERVIMRILWGLDLLIEKQVFHPREVAHSLVCSDQLRVESAAFFRCAFKLLLKLIFFLDYKSNREVFNKSIEKSSQF-MDSPADDRFNLRFIDPAVELIQLLLSREMCLMPAYFAMHELIRSF-PVELSGSPHKAIQDSVASFMDSFARPARLLYSQIHQSIKPICGITPPTTPGFRLDSTNLLL-TVVKGAMRYPDFLTSSQTPLLCFVLGQLRSTELVAGMLDPSTGKSKTRSIGLEKAIVDWIIELMEITESTDDRT----WVRTTFEHLISVVITFMLSSTIRFSEIVVKLNTKLEGRKWTKSSHYVMWFVLNVTSGFIGKNMLTDFVPCLRLFDILFPDAEDPDRAVELQTLGCGKQTDRRQPDGTSDADVTLIPTKDNSDSEASRPGRGGDEENVTTNTKDTGMECDADSGDEVYLSSTSADESDDASSLAILPPTRHRTAASRRRHCRLMHLATSPVCLWQHVLRKSRTGHDHLPRVMPPALRPTEARISYRMRILLDVIVSTDPTGSISPVLAQLTWRQLFIILNAYSTDNEASQLIQHLVDMFWFNGEEDPGNPSTFSRLWPKLIENGSVFLPYGLVAHGRLRPLSHHLIRIMSIHLRMLVLHLLLQKFANLQYSDELTTPAVVETMCRIVACREVEPSNTGRIL--SLLPK----LLPGNAGNQAATDTSEPKTASSSCNLLSTLLDTLGYRLVDTFLPEVKVQTIVTLVNLLRMWTSWSASETPIEDQSQIGKDGKLQSSSKNDATEIPVELFYMLEWTIVKLARCLQAPDCVIYGLSSAISP 861 HSP 2 Score: 109.383 bits (272), Expect = 6.138e-20 Identity = 59/158 (37.34%), Postives = 87/158 (55.06%), Query Frame = 3
Query: 4014 LWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPL----QADKTGKLIELVAELYNC-------LAQVPK------------------------SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMP 4382
LWH++WSH N TQ+T+ + E I P ++ E Q L++ ++ +PLM L A++ KLI+L AELY LA +P +P++H+D IADLMYH+KY+YVG+GVL+ V+ +P LR +LKFI+P
Sbjct: 1674 LWHAVWSHTNTTQLTSLPSLFAERILPRLKNEAQLLMSFYLVSPLMGTLYAERHAERHAKLIDLTAELYRAVLQVDSHLAAIPDRSTHHPGEEVRANTEADASPPNNTGVPMVHADAIADLMYHIKYMYVGNGVLDQVRPILPNLRPCLRKRLKFILP 1831 HSP 3 Score: 98.5969 bits (244), Expect = 1.134e-16 Identity = 110/489 (22.49%), Postives = 202/489 (41.31%), Query Frame = 3
Query: 2250 PQTVPPKIGRQ--------RLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSIXXXXXXXXXXXALLPVRFNVLQIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVS-SSYFYCVALRLI-----LEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNR-NFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGN---------PCHLD-----NWYKIHFALHEKLPEVMIN--SNNNNQII--------------LPQYYNHLVLRLIPVLELMISSLVHFPQIAL---KMTSFFSVASKLFKFHSYPLYFCYTLIRS--------------------------QIMWGPENRRFLQIIVHHILQEHQ 3494
P V P +G ++ + E+N+FI+ +L+Q+Y A++++E + KNF++E ++T + R + D+P + S L + AS +++ CG G N + +++ + Q+ C+ ++ W Q+ + + F CV LR + L V+ ++ + L R +R LCDYA+ + T S + ++K + AL + ++ DRFL+ L+ N+QT V +I +F+K + +L + P + P H D +W LH+ +P+ + S +N + LP Y H++LRL+P+LE + L HF + +L ++ F S LF+FH PL CY L+R ++MW + R +Q+I++H+L++H
Sbjct: 934 PSEVSPSVGSALSNAISGYPFLMTEYVELNRFIIFSLLQIYYAFDLDESSAMKNFLAEQIRTMCE------------------RCGRASLTDLPRLVSSRLPEFLVHL----RQRASPNMSGPGCGFQA-GELVDKRTNLSQLPHLVLEEFTQL---CDGETT----WSQIAARGNLFLCVLLRYVAAHGMLPKSAVNTLLRLPFGQLIVR-----VRTLCDYAVTCL----TGSPSGDECMAQKCLPALCELCVGLRVVPLDRFLLVLLTHTNYQTSQMDAVHRIIVHLFTKCDRLSEAIAYLSSRFPTVPQDTAIPSVESIPLHPDPVSSRHWPDTVLRLHQLIPDQWVTEISGDNPGMSKSTSETGEVRSNPKLPVLYGHMLLRLLPILEAI---LAHFLETSLPLVQLIPFCHAISPLFRFHRRPLNMCYVLLREHWHLTPPELEAFLTRDIPGTNSMNDKRMMWLADRLR-VQLIIYHVLKDHH 1379
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Match: A0A1D2NIV8 (Mediator of RNA polymerase II transcription subunit 23 OS=Orchesella cincta OX=48709 GN=Ocin01_01498 PE=4 SV=1) HSP 1 Score: 261.151 bits (666), Expect = 6.861e-67 Identity = 308/1453 (21.20%), Postives = 597/1453 (41.09%), Query Frame = 3
Query: 150 NTAISDSWRVISKDDKPAALKFLIQVIVD------RNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPANH-------------WHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNET-AMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIF--SXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPT---NQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR--QRLMIGDSEEVNKFIVITLIQLYKAYEMEEVL--SWKNFISETVKTFQQSIXXXXXXXXXXXALLPVRFNVLQIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVN-FLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLI---RSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAE-------YINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIP----ILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
NT S +V+S+ P +++ I+ I+ N + +L KIL + + S + + L+ SE L + N F+ + I ++P +DYK RE+ S++K+ LFP I + L++ +++++ + R L+PAYF ++E++K + PE K + PH L ++ F+ SF+ A+++ R PVV +HS S+ + +T V KG + Y ++ + Q +L Q S D+ + L K + S LE+ +L+ M + EA N AN W W +HL ++ F+ F I ++ K + FK R ++ W ++ SG I KN L DF+P L+ +D+LYP D DI + + + M A +W+H+++K+++ L R LP AL+ E + + NV + ++ NAFST +E + +G + E + G S S+ + S +I+ +++VH + H ++ I S ++ N++P ++E++ RL+++ E++ + F+++ +LP +Q G + HT+L++ +R +++ P + L + + + Q+ ++ L+L F D+ T ++G + L+ +SEE+N+ +V+TL + + + N+ + + Q+ + + S + A PV ++ Q +P+ NKQ+ +N++ + A + + E + + F C+ +++LE V+ V + + + +LR CDY + Y VN K+ +A+ I+K NII FDR L+ L R+ + + +V F +I+ + K F + + F+ + P +W++ H + + K PEV Q +P Y+ ++ LR +PV ++MI + I + S L+KFH P+ + Y + S++ P ++ L + L++ + +N + L S ++++ I+ L+N+ V KF P +WR ++ + HV++A +E+M +P + I+ + R E + N G ++ + Y + I ++ + +F + + L + H++W H + + + I + + ++ E Q L H+ AP + L ++ + L Y L +V KSI + + D DL+YH+KY+Y G V V+ + L+ + +FI
Sbjct: 62 NTLQSKLAKVLSEGTSPESVERAIKHIISITGQPTNNDETVEILMKILEKLVASSNVSARLVCEALLESERLTIRNSKFWINSFVLIKKIIPGVDYKGVREIMKISLDKAALFPEKILPSQLPQLNAVVRLLQYIFDRNACLLPAYFIVNEILKNY-PE-KRQFPHWKLSQLLSDFVDSFQPIAQMVTVIGRSRMLPVVEHSHSGYSLLSWKLDPVTLRFVVKGNLPYDKDLLQPQDRLLRFILEQPYSRDMVCAILGLQKQLKIRCSTLEDRLVELVIVAMEKTEAEMNQANDPQSGYDEISAATLWLW-----QHLSTQLIFFVLFQLASFPHIVMSIHNKLSEIKLFKGRDFLMWVLLQFISGSIQKNSLGDFLPVLRLYDLLYPEKDALVVPDITKP--------------------YCVSQMAA-------TCIWIHILKKAQTENQFLCRPLPTALKKQYEYLQSIAVQNSTQPSLNVCD----FRVALLANAFSTNSELFSRPMGALVEAMHGSTSSSVSSQISVAPLSV------------------------QILDSLTVHTKMSLIHNIVTHIMKISTTKSGQNLNPALVETYARLLVYLEIESLGIKGFMSQ---------LLPNVFKSQAWG------------ILHTLLEMFCYRL-HHIQPHYRVQLLSHLNALANNAHSNSAQLHMCVETTALRLITSFNSVDV-------HTQFSRVGGDCKSLVSSESEELNRALVLTLARAIHVTGSDTIAGSGGSNWYRDILTAIMQN-TPHSWSTHVLQAFPPVVYDFFQQNQVPKE------------------------------------------NKQQLKNMVDEEYRNWNAMISNEKELIEHFASQGTPPLFLCILWKMVLETDRVNPVAPKVIERIGPKGISANLRKFCDYVVCEFANAGAGQY-VN-----KLADAISDLIWKFNIIPFDRLLLCLALRSHEGNEAQVCFFIIHMLLIKPLEFKNRIQEFVRDNSPEHWKQ----TSWHEKHKSFNAKYPEVSY-FEGQQQNPIPMYFGNVCLRFLPVFDIMIHRFLEMNNIQKSLELMLEQMSPLYKFHDRPVTYLYNTLHYYESRLRDKPSLKKKLISAILGALRDIKPTNWALSDQYQTFLQGSNEDINWAPDIDYYAGLINRFVDVIEGRRKFFAPV-DWRFNEFPNPPAHVLHATCVEIMALPVTPTVAANGILEVMMCSRAEMDANEANMDVESWFNSTGLILVTLPESYWLLLHERIVSTIEKLSTWSHKYTPLQLFNF-------KTVKNGFLYSEYANLLAITHAVWYHLGIGHMHHIQTLITDKLSSIVQNEIQLLFIGHLVAPFLQRLNTERPNMVCRLTVAFYELLEKVDKSISKDACLTYMDVFCDLLYHIKYMYTGDVVKTDVEPIIGRLRPALQLRFRFI 1361
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Match: A0A091LVN6 (Mediator of RNA polymerase II transcription subunit 23 (Fragment) OS=Cariama cristata OX=54380 GN=N322_01180 PE=4 SV=1) HSP 1 Score: 257.684 bits (657), Expect = 8.090e-66 Identity = 309/1444 (21.40%), Postives = 610/1444 (42.24%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISET-VKTFQQSIXXXXXXXXXXXAL--LPVRFNVL-QIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQV-ENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS-EPELDY----DKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
A W +S++ ++++++ I +++ + L+ L++ +E + + + +L+NS++L E + + + + ++ +DYK R++ +EK PN +SS + L + +V+ +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+ S + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + + T +P N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L S++ PQ + ++ +SEE+N+ +++T L +A + + + + I T K Q+I N ++ L P V + ++P+ FNL ++E+ + EN I A+ + Q SS F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + E + +VH I+ + NR P GW L L C+ P D D T C+ + + + + G F P +WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y L + S + + D I D +YH+KY++ G V + V++ + L+ +L+FI
Sbjct: 30 AFRQFWSSLSQESHEQCVQWIVRFIHNQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVVAYILERNACLLPAYFAVTEIRKLY-PEGK--LPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAMCNSWKLDPTTLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAHSDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGVLVETIYG-------------NGNMRTPLPG----TNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEV-----QPQFTRFLSDPKTVLSAESEELNRALILT---LARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQVFFKQNNVPQESR----------------------------------FNLKKNVEEEYRKWKSLTNENDIIAHFSVQG----------SSPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMSYHKKYPEKLYFEGLAEQVNPPVQIQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEGHLRERPNLKRKLVHAIIGSLK-DNR---PLGWCLSDTYLKCAMNPREDNPWIPDDTY-YCKLIGRLVDNILKSPGPF--PNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVINSPSLTSETEWVGYPFQLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLIPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYEMLLNADRYSSHLNYMDPICDFLYHMKYMFTGDSVKDQVEKIICNLRPALKLRLRFI 1318
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Match: A0A2I0MUI7 (Mediator complex subunit 23 OS=Columba livia OX=8932 GN=MED23 PE=4 SV=1) HSP 1 Score: 256.914 bits (655), Expect = 1.263e-65 Identity = 309/1447 (21.35%), Postives = 609/1447 (42.09%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVI-VDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISET-VKTFQQSIXXXXXXXXXXXAL--LPVRFNVL-QIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIIT-QVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS------EPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPK-SIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
A W +S++ ++++++ I + + L+ L++ +E + + + +L+NS++L E + + + I ++ +DYK R++ +EK PN +SS + L + +V+ +L R L+PAYFA+ E+ K + PE K PH LL ++ F+ +FR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+ S + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + S N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LPT K + HT+L++ +R +++ P + L ++ +VP Q+ ++ L+L S++ PQ + ++ +SEE+N+ +++T L +A + + + + I T K Q+I N ++ L P V + ++P+ FNL ++E+ + EN+I ++ + Q SS F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P ++W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + E + +VH I+ + NR P GW L L C+ P + D I C+ + + T+ GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I P + + E+V F F D + H L L H++W H+++ Q++ ++ E + P ++TE Q L H+ P + Q ++T +IE+ Y L + S + + D I D +YH+KY++ G V + V++ + L+ +L+FI
Sbjct: 19 AFRQFWSSLSQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDTLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVVAYILERNACLLPAYFAVTEIRKLY-PEGK--LPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAMCNSWKLDPTTLRFP--LKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHAFAMT----CIWIHLNRKAHSDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLQMNDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGNMGISLPGT-----------------NCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPT---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSNLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEV-----QPQFTRFLSDPKTVLSAESEELNRALILT---LARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQVFFKQNNVPQESR----------------------------------FNLKKNVEEEYRKWKSMNTENEIISHFSAQG----------SSPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----NDWHTKHMSYHKKYPEKLYFEGLAEQVNPPVQIQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYELHLRERPNLKRKLVHAIIGSLK-DNR---PLGWCLSDTYLKCAMNAREENPWIPDD--IYYCKLIGRLVDTM---AGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVINSPSLTSETEWVGYPFQLF-----DFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTELLIPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYEMLLNADRYSSHLNYMDPICDFLYHMKYMFTGDSVKDQVEKIICNLRPALKLRLRFI 1308
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Cavefish
Match: med23 (mediator complex subunit 23 [Source:NCBI gene;Acc:445483]) HSP 1 Score: 130.183 bits (326), Expect = 7.719e-30 Identity = 154/741 (20.78%), Postives = 316/741 (42.65%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVIVDRNI-NNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFC----RHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEA----CDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQ 2342
A W + ++ ++++++ I ++ + L+ L++ +E ++ + + L+NS+SL E + + + I ++ +DYK R++ ++K PN +SS L +V+ +L R L+PAYFA++E+ K + PE +++ ++ F K ++ C R PVV+ + + + +KLD +L +P KG + Y ++ Q +L Q S D+ + L K + + LE+ L+ M + E D + W +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL RK+++ + L +P +L+ E + Q + N M+ +++ ++ NA+ST +E L +G + E ++G + + S N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LP K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L S+ + PQ + ++ +SEE+N+ +++TL +
Sbjct: 19 AFRQYWSSLPEESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCQALINSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKVQTIPNLVSSAVVHQLLPVREVVEYILDRNACLLPAYFAVNEIRKLY-PE-----------GQLSHWVVFF---WKCVVCVCFVLGRCSLLPVVNNSGAICNSWKLDPATLRFP--LKGMLPYDKDLFEPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCTVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLW-----QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVSDINK-----------------------PQSTHAFAMT----CIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLR--------NKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGTQRISLPGT-----------------NCIACGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPN---------VFKCHAWGILHTLLEMFSYR-MHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSE-----VQPQFTRFLSDPKTVLSAESEELNRALILTLAR 661 HSP 2 Score: 125.946 bits (315), Expect = 1.638e-28 Identity = 120/569 (21.09%), Postives = 245/569 (43.06%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDY-----DKTIEI------CRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVP-KSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + + +VH I+ + NR TP GW L SE L + D + I C+ L+ ++H + + GK P+ N WR ++ + H ++ +ELM + V +++N+ ++ C ++ + Y + +I P++ + + L D + H L L H++W H+++ Q++ ++ E ++P ++TE Q L H+ P + Q ++T ++E+ Y L V S + + D I D +YH+KY++ G V + V++ + ++ +L+FI
Sbjct: 753 SPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQ----SDWHNKHMSYHKKYPEKLYFEGLAEQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLK-DNR--TP-GWCL---SETYLKFGMNPRDDNVWIPDDTYYCK-LIGRLHVLPMA-GKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKRS------------ECTCSVLQEPYWIVLHDRLVSVISSPVLTSEMDCVGYPFQLL----DFTACHQSYSEMYCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPLVKTEFQLLYIYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDHHSKHLAYMDPICDFLYHMKYMFTGDSVKDQVEKIICTLRPAMKLRLRFI 1286
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Sea Lamprey
Match: med23 (mediator complex subunit 23 [Source:NCBI gene;Acc:445483]) HSP 1 Score: 132.109 bits (331), Expect = 3.274e-31 Identity = 127/567 (22.40%), Postives = 247/567 (43.56%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVN-FLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGW---RLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKN--WRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQ-EFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQ--LCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPIL-HSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
SS F C+ +++LE ++ V + + R +R+ D+ + S +VN + +E L ++K NI++ DR ++ L R + + +V F +I + K F + VN F+ E P ++W+ H + H+K PE + Q+ LP Y+ ++ LR +PV +++I + P +A + + L+ P+ + Y E + +V +L Q NR P GW + S E + +LM ++ V GK P+ N WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + ++ P++ + E+V F F D + H G++ L L H++W H+++ Q+T +++E ++P ++TE Q L H+ P + ++T ++E+ E Y L V ++ + H D I D +YHVKY++ G V + V++ + L+ +L+F+
Sbjct: 497 SSPLFLCLLWKMLLESDHIRLVTYKVLERIGARALAAHVRMFADFLVYEFST-SVGGQQVN-----RWIEILNDMVWKYNIVTLDRLVLCLALRCHEGNEAQVCFFIIQLLLLKPSDFRNRVNDFVKENSPEHWLQ----NDWHAKHMSYHKKYPEKLYFEGLAEQVNPPVAMQPQYLPIYFGNVCLRFLPVFDIVIHRFLELPPVAKSLETLLDGFPALYPT-DRPITYLYNTFHYYERKLREQPSLKRKLVQTVLGS-QKDNR---PQGWCLSEMYLNSRREGSWTPDDSYYCKLMARL--VDNMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPAKDVGNALVNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWMVLNDRVVGVMNSPLLAHESEWVGFPFQLF-----DFTSCH--QAYGEMYCSYVLALAHAVWHHSSIGQLTLIPRFLKEVVKPLVKTEVQLLYVYHLVGPFLLRFLQERTRCMLEIAVEFYEMLQAVDQNCKHMGHMDPICDFLYHVKYMFTGDSVKDSVEKIICTLRPALQLRLRFV 1039 HSP 2 Score: 65.0846 bits (157), Expect = 1.268e-10 Identity = 59/247 (23.89%), Postives = 106/247 (42.91%), Query Frame = 3
Query: 912 KSQNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWG 1649
+ Q C LE+ L+ M + E ++ + + +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P AF + +W+HL +K++S + L +P +L+ + + Q + N M+ +++ ++ NAFST +E L +G + E ++G
Sbjct: 2 QKQRCPV--LEDQLVDLVVVAMERSETEEDRLDDGGASQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDISK-----------------------PQSTHAFAMTC----IWIHLNKKAQSDNSKLQIPIPHSLKLHHDFLQQSLR--------NKTLTMTDYKIALLCNAFSTNSEYFPLPMGVLVETIYG 211
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001232.1 (pep scaffold:Pmarinus_7.0:GL501116:79099:87632:1 gene:ENSPMAG00000001104.1 transcript:ENSPMAT00000001232.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 84.3445 bits (207), Expect = 8.785e-18 Identity = 73/304 (24.01%), Postives = 140/304 (46.05%), Query Frame = 3
Query: 54 LQRLIDNLIDNINLTYDIYQPIKKPTTDDKWIN-----TAISDSWRVISKDDKPAALKFLIQVI-VDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQN--CKYSGL 941
LQ + D++I L + + + T DD+ A W ++++ ++++++ I + + L+ +++ +E ++ + + TL+NSE L E + + + + ++ +DYK R++ +EK P +SS + L + V+ +L R L+PAYFA+ E+ K + PE K H LL ++ ++ SFR A++ R PVV+ + + + +KLD +L +P KG + Y ++ Q T L Y+ E P S++ C GL
Sbjct: 5 LQSIFDDVIKT-ELIEEAFAGMFMDTEDDEKTKQITCLAAFKLYWSSLTQESHEQCVQWVVRFIHTQHSPKRISFLYDLIATAVETSLLPPRLVCETLINSEHLEYEKAQLWSLTFKLVRKIIGGVDYKGVRDLLKAILEKIQTIPITVSSSVVQQLQTARDVVMYILDRNACLLPAYFAVTEIRKLY-PEG--KLGHWLLGGLVSDYVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFP--LKGLLPYDKDLFEPQ-------------TALLRYVLEQPYSRDMVCNMLGL 289
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Nematostella
Match: EDO35076 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMH7]) HSP 1 Score: 102.449 bits (254), Expect = 9.184e-24 Identity = 74/264 (28.03%), Postives = 127/264 (48.11%), Query Frame = 3
Query: 516 LKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIE----ACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREA 1295
++V++ +L RK L+PAYF ++E+ K F PE K PH L ++ ++ SFR A ++ R P++ S+ ++KLD +L++P KG + Y+ ++ + Q + ++ Q S ++ + L K Q + LE LI M + E D+ A W +HL ++ F+ F + L K R+ + R ++ W ++ SG I KN + DF P +K F++LYP N+ DI A
Sbjct: 1 MQVVQYILDRKACLLPAYFVINEIFKDF-PEGKAT-PHWSLSKLLSDYVDSFRPLAHMVTVTGRPLLLPIIGHPSSSIGVWKLDPATLSFP--MKGLLPYNKSLFKPQKNLLQYVIGQPYSREIICSMLGLNKQQKQRCLALEELLVDLIVIAMEKSEHDQSGLDDTACQLLW-----QHLSSHLIFFVLFQFASFPHMVLALYDKLQGRNLRQGRDHLMWVLLQFISGSIQKNPISDFKPVIKLFELLYPGNEPLPEPDINHA 255
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Nematostella
Match: EDO32385 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV64]) HSP 1 Score: 88.5817 bits (218), Expect = 9.035e-20 Identity = 50/150 (33.33%), Postives = 79/150 (52.67%), Query Frame = 3
Query: 3930 FTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPIL-HSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
F DF + S+ H D + L H++W HA++ Q+T +R I P + E QFL C + P + Q ++T L E+V ELY+ L ++ K L H D I D MYH+KY++VG + E V++ +P L+ +L+F+
Sbjct: 52 FFDFETNVAMHSETHADYVMA-------LTHALWHHASIGQLTLVPQLLRSRISPLVGNEAQFLYLCRLVGPFLQRFQQERTRCLQEVVIELYHLLEKIDKEAQHLYHIDIICDFMYHIKYMFVGDLIREQVQKVIPMLRHSLQVRLRFM 194
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Medaka
Match: med23 (mediator complex subunit 23 [Source:NCBI gene;Acc:101160525]) HSP 1 Score: 152.525 bits (384), Expect = 1.226e-36 Identity = 163/743 (21.94%), Postives = 326/743 (43.88%), Query Frame = 3
Query: 156 AISDSWRVISKDDKPAALKFLIQVI-VDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKVIRKLLSRKNNLMPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMG-----MIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAML-IGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISI--FSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGR-----QRLMIGDSEEVNKFIVITLIQ 2342
A W + +D ++++++ I R+ + L+ L++ +E ++ + + L++S+SL E + + + I ++ +DYK R++ ++K P +SS + L + +V+ +L R L+PAYFA+ E+ K + PE + H +L + I+ F+ SFR A++ R PVV+ + + + +KLD +L +P +G + Y ++ Q +L Q S ++ + L K Q + LE L+ M + E H+ +G + +HL ++ F+ F + L+ K A R K R ++ W ++ SG I KN L DF+P +K FD+LYP + DI + P +F + +W+HL RK++S + L +P +L+ E + Q + N GMS +++ ++ NA+ST +E L +G + E ++G + S T +P N G +P ++ +++VH + H + + + ++ ++P ++E++ RL+++ E++ + FI++ +LP K + HT+L++ +R +++ P + L + +VP Q+ ++ L+L + L V P+ R + ++ +SEE+N+ +++TL +
Sbjct: 39 AFRQYWGTLPQDSHEQCVQWIVRFIHTQRSPKRISFLYDCLAMAVETSLLPPRMVCVALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKGVLDKIQTVPITVSSAILQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLY-PEGQLS--HWILGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFP--LRGMLPYDKDLFEPQTVLLRYVLEQPYSREMVCNMLGLNKQQKQRCPVLEEQLVDLVVYAMERSET----EEHFDADVGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINK-----------------------PQSTHSFAMT----CIWIHLNRKAQSDNSKLQIPIPHSLKLHHEFLQQSLR--------NKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYG-------------NASVRTSLPG----TNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQ---------LLPN---------VFKSHAWGILHTLLEMFSYRM-HHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRL----------ITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLAR 691 HSP 2 Score: 130.954 bits (328), Expect = 4.413e-30 Identity = 122/570 (21.40%), Postives = 246/570 (43.16%), Query Frame = 3
Query: 2742 SSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTV-NFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQI---------ILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRL----LACS----EPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLC-QEQRGGPK--FAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDW---DSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPIL-HSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFI 4376
S F C+ +++LE +++ + + + R +R D+ + S ++N K +E L ++K NI++ DR ++ L R+ + + +V + +I + K F + V +F+ E P +W+ H H+K PE + +Q+ LP Y+ ++ LR +PV +++I + ++ + + L+KFH P+ + Y + + + +VH I+ + NR TP GW L + C E + C+ + K T G F P +WR ++ + H ++ +ELM + V +++N+ + Q P+ ++N G ++ + Y + +I P + + TD++ + D + H + L L H++W H+++ Q++ ++ E ++P ++TE Q L H+ P + Q ++T ++E+ Y L V ++ L + D I D +YH+KY+Y G V E V++ + ++ +L+FI
Sbjct: 783 SPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFST-SAGGQQLN-----KCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ----SDWHSKHMNYHKKYPEKLYFEGLADQVNPPIQQQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLK-DNR--TP-GWGLSETYMKCGMNAREDNVWIPDDTYYCKLIGRK------TPGPF--PNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRESITAWMNAIGLVITALPEPYWVVLHDRIVSVISSPALTSE-------TDWVGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLTPKFLSEVLKPIVQTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQNCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFI 1323
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Match: SMESG000056209.1 (SMESG000056209.1) HSP 1 Score: 1561.2 bits (4041), Expect = 0.000e+0 Identity = 818/823 (99.39%), Postives = 822/823 (99.88%), Query Frame = 3
Query: 918 QNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIFSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSIXXXXXXXXXXXALLPVRFNVLQIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIMEALETFIFKINIISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHS 3386
+NCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIFSEPQNINNISPQIIESFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSISGNGNSNNTNNALLPVRFNVLQIKDIP+SLLSSLSGLISCSPSIENSN SDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKI+EALETFIFKIN+ISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHS
Sbjct: 161 KNCKYSGLENAFPQLIYNVMLQIEACDNPANHWHWIMGMIEHLVCSVLNFLHSMSIGFSEIFTKLNAKAANRSWFKSRKYIWWFVINATSGFINKNMLEDFVPCLKFFDILYPSNDKTWSDDIREALKSFGDNTTRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIFSEPQNINNISPQIIESFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSISGNGNSNNTNNALLPVRFNVLQIKDIPDSLLSSLSGLISCSPSIENSNTSDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEKIIEALETFIFKINMISFDRFLIFLMNRNFQTDTWKVVFCLINDMFSKWPAFISTVNFLHEQCPNTLGNPCHLDNWYKIHFALHEKLPEVMINSNNNNQIILPQYYNHLVLRLIPVLELMISSLVHFPQIALKMTSFFSVASKLFKFHS 983 HSP 2 Score: 326.635 bits (836), Expect = 1.262e-93 Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 3
Query: 39 MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLK 521
MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLK
Sbjct: 1 MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLK 161
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Match: SMESG000054160.1 (SMESG000054160.1) HSP 1 Score: 885.174 bits (2286), Expect = 0.000e+0 Identity = 495/541 (91.50%), Postives = 502/541 (92.79%), Query Frame = 3
Query: 1320 TRRGNLETNIMEHPPNGMLAFFLDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKPVIIPNEIISTMSVHVRFLFNHLLMISIFSXXXXXXXXXXXXXXXFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSIXXXXXXXXXXXALLPVRFNVLQIKDIPEXXXXXXXXXXXXXXXIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFSSEK 2942
TR +L + E P + +DTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKP I S F F FSEPQNINNISPQIIESFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSISGNGNSNNTNNALLPVRFNVLQIKDIP+SLLSSLSGLISCSPSIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVF ++
Sbjct: 105 TRSTDLAGYLYELPKSQ-----VDTGPLVLWMHLIRKSKSLPTNLPRNLPPALQPMCEVVTQEISRLEGYDCFNVLAGMSWHELIMILNAFSTKNETAMLIGRINEYLWGIKSHQSSASSIILDQSTITDIPTFILPYNIRGQGKP-------IPFQSFADDFHF--------FSEPQNINNISPQIIESFCRLILHREMDIQNLSKFINKSFLLKGTTLILPTNQTNGQSSPGNKLSEKRLFHTMLDLLIHRCSYYLTPDIAINLWFSIILNISVPNELPLQIQFQIDFGILQLARLFQKSDLVLYGLSPQTVPPKIGRQRLMIGDSEEVNKFIVITLIQLYKAYEMEEVLSWKNFISETVKTFQQSISGNGNSNNTNNALLPVRFNVLQIKDIPDSLLSSLSGLISCSPSIENSNASDSINETICGNTGFGPFNLSILNKQEFENIITQVENQIPANCNYQSCSWEFWRQLVSSSYFYCVALRLILEWRNVSNVVMMSADLLNRRMSDQSLRVLCDYAIANIVLHSTQSYRVNVFLQKR 625 HSP 2 Score: 258.455 bits (659), Expect = 2.645e-73 Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 3
Query: 558 MPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQ 920
MPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQ
Sbjct: 1 MPAYFAMHELMKCFCPEDKEKQPHKLLHAEINAFMSSFRKPAKLLIPFCRHFFYPVVSITHSTNSIFKLDGNSLTYPNVSKGAILYSDNVSRAQLDECTKLLIQTRSTDLAGYLYELPKSQ 121
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Match: SMESG000054164.1 (SMESG000054164.1) HSP 1 Score: 714.531 bits (1843), Expect = 0.000e+0 Identity = 341/352 (96.88%), Postives = 343/352 (97.44%), Query Frame = 3
Query: 3339 MTSFFSVASKLFKFHSYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN 4394
M SFF+ + L YPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN
Sbjct: 1 MLSFFTNNNLLTI--GYPLYFCYTLIRSQIMWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN 350
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Match: SMESG000054164.1 (SMESG000054164.1) HSP 1 Score: 682.559 bits (1760), Expect = 0.000e+0 Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 3
Query: 3429 MWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN 4394
MWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN
Sbjct: 1 MWGPENRRFLQIIVHHILQEHQLSNRCFTPTGWRLLACSEPELDYDKTIEICRELMNKMHTVFLTYGKFGLPYKNWRQDQSSSAQCHVVYAAVIELMLCGKSPQIVVESIINLCQEQRGGPKFAEYINIFGCMMALFSKEYRQYATHQFSKIIIDPIVKCQEFVRMFFTDFLQDDWDSSKIHPDNQIGDLQVQLCLWHSMWSHANMTQITNCVNYIREFIRPFIETEGQFLLTCHMCAPLMYPLQADKTGKLIELVAELYNCLAQVPKSIPILHSDTIADLMYHVKYIYVGHGVLEYVKERMPYFEEELRAKLKFIMPQPSN 322
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Match: SMESG000056209.1 (SMESG000056209.1) HSP 1 Score: 328.176 bits (840), Expect = 7.654e-104 Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 3
Query: 39 MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKV 524
MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKV
Sbjct: 1 MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKDDKPAALKFLIQVIVDRNINNLHVLFKILSVFIEENIYSCKDIACTLVNSESLNVENKHFFRMCYSYINILLPSLDYKANREVFNKSIEKSFLFPNDISSEERRSLHSCLKV 162 The following BLAST results are available for this feature:
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Mediator of RNA polymerase II transcription subunit 23 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006873 ID=SMED30006873|Name=Mediator of RNA polymerase II transcription subunit 23|organism=Schmidtea mediterranea sexual|type=transcript|length=4458bpback to top protein sequence of SMED30006873-orf-1 >SMED30006873-orf-1 ID=SMED30006873-orf-1|Name=SMED30006873-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1453bp MLTSGLQRLIDNLIDNINLTYDIYQPIKKPTTDDKWINTAISDSWRVISKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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