MAP/microtubule affinity-regulating kinase 3
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006834 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Match: A0A0V0J1G0 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR100555 PE=4 SV=1) HSP 1 Score: 117.087 bits (292), Expect = 1.212e-27 Identity = 77/248 (31.05%), Postives = 129/248 (52.02%), Query Frame = 3 Query: 33 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEI-----------------VKIDNVCDYPPTLGKYIRKNYLH-NQFNPK------ILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 704 M I+ +G+ K+L EI +NLK+ G+GR+ SE+ Y EPC+Y++K + M+ E R R AER+ RG+ +G+V+I +Y P W L++ + ++ + VK+ + PP++ + RK N + K +L K F+ F+ EP+ ++K++IFP YE AADGL++KK T+ IYYI+ G++++V L +K ED+L ++A Sbjct: 1 MTNIKFIGRPSSFYGKYLSEISRNLKSRGIGRVFVKESESKTYSEPCFYVMKKIEPLMSDESGVRCRAFAERVFRGRHLGLVHISKSYEPDWRLLSIEEGRRLQESASQMTNVVQDNKVPCVAAMPPLLAVKLQRLGKIPPSVVEAARKVECPVNSASAKEANGFLLLTKHFDDPTIFQVPIEPTTEEKSRIFPSYEVQAADGLILKKKTD-KNIYYIRRSDTPGLRWRVELAQKD---IEDELLQDA 244
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Match: A0A183T8E3 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1) HSP 1 Score: 112.079 bits (279), Expect = 6.726e-26 Identity = 73/231 (31.60%), Postives = 120/231 (51.95%), Query Frame = 3 Query: 84 LGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEI-----------------VKIDNVCDYPPTLGKYIRKNYLH-NQFNPK------ILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 704 L EI +NLK+ G+GR+ SE Y EPC+Y++K + M+ E R R AER+ RG+ +G+V+I +Y P W L++ + ++ + VK+ + PP++ + RK N + K +L K F+ F+ EP+ ++K++IFP YE AADGL++KK T+ IYYI+ G++++V L +K ED+L ++A Sbjct: 1 LSEISRNLKSRGIGRVFVKESETKTYSEPCFYVMKKIEPLMSDESGVRCRAFAERVFRGRHLGLVHISKSYEPDWRLLSIEEGRRLQESASQMTNVVQDNKVPCVAAMPPLLAVKLQRLGKIPPSVVEAARKVECPVNSASAKEANGFLLLTKHFDDPTIFQVPIEPTTEEKSRIFPSYEVQAADGLILKKKTD-KNIYYIRRSDTPGLRWRVELAQKD---IEDELLQDA 227
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Match: A0A3P7D1V0 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS12741 PE=4 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 1.316e-25 Identity = 72/231 (31.17%), Postives = 120/231 (51.95%), Query Frame = 3 Query: 84 LGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEI-----------------VKIDNVCDYPPTLGKYIRKNYLH-NQFNPK------ILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 704 + EI +NLK+ G+GR+ SE Y EPC+Y++K + M+ E R R AER+ RG+ +G+V+I +Y P W L++ + ++ + VK+ + PP++ + RK N + K +L K F+ F+ EP+ ++K++IFP YE AADGL++KK T+ IYYI+ G++++V L +K ED+L ++A Sbjct: 1 MSEISRNLKSRGIGRVFVKESETKTYSEPCFYVMKKIEPLMSDESGVRCRAFAERVFRGRHLGLVHISKSYEPDWRLLSIEEGRRLQESASQMTNVVQDNKVPCVAAMPPLLAVKLQRLGKIPPSVVEAARKVECPVNSASAKEANGFLLLTKHFDDPTIFQVPIEPTTEEKSRIFPSYEVQAADGLILKKKTD-KNIYYIRRSDTPGLRWRVELAQKD---IEDELLQDA 227
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Match: A0A5J4NY78 (Small subunit ribosomal protein S34 OS=Paragonimus westermani OX=34504 GN=DEA37_0003520 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 1.490e-24 Identity = 73/245 (29.80%), Postives = 121/245 (49.39%), Query Frame = 3 Query: 33 MPIIQHVGKKCVTNY-KFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVK---IDNVCDYPPTLGKYIRKNYLHNQFNPK---------------------ILMKPFEKYMDFKSVN---EPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTE 683 MP +Q+VG+ ++ Y K+L EI KNLK GVGR+V +E A + EPCYY+L + M+ + R R +RI RG+ +G V ++ T+ P W L++ ++ + L D + + I V PP L ++R+N + K +L + +E FK V P+ ++++I PPY ++D LV K+ + F YYI+ G+ +KV + + + TE Sbjct: 1 MPRVQYVGRPSLSFYGKYLSEIAKNLKTRGVGRVVVKETETAKHMEPCYYVLSRIEPLMSDTSNVRCRAWGQRIFRGRDLGAVRLEGTHEPDWRLLSTTEANALLALAADSKQSAQDEHISCVAPMPPLLALHLRRNGRFPESTLKAVEGLPKCDTPETVREASGFLLLTRMWEDPTSFKVVQVPLTPNSNEQSRIHPPYTVASSDDLVRKRNLDRDF-YYIRRSDTPGLYWKV-ITTRSSIQTE 243
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Match: A0A074Z9U0 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_11346 PE=4 SV=1) HSP 1 Score: 107.071 bits (266), Expect = 9.799e-24 Identity = 73/252 (28.97%), Postives = 117/252 (46.43%), Query Frame = 3 Query: 33 MPIIQHVGKKCVTNY-KFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIV-KIDNVC--DYPPTLGKYIRKNYLHNQFNPKI------------------------LMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 704 MP IQ++G+ + +Y K L EI +NLK GVGR+V +EA+ Y EPC+Y+L+ T ++ + R R RI RG G+V + TY P W L+ + L D K+ + C PP L ++R+ H++F I L K ++ + EP+ Q++ +I+PPY+ + GL+ K+ + PF YYI+ G+ +KV + P + N Sbjct: 1 MPRIQYIGRPSLHHYGKLLSEIVRNLKTRGVGRIVVKETEASRYPEPCFYVLERTIPLLSDSSNVRCRAWGRRIFRGHDFGVVLMTNTYEPDWRLVPTCEATALLDRAADPKNAAPKLTSKCVAPMPPLLTLHLRR---HSRFPDNIIRAVSESVDPTLPQATREASGFLLLTKLPDEPTSLEIPTEPTVQEQRRIYPPYQVASEGGLIRKRELDRPF-YYIRRADTPGLLWKVVTNKDSDDPKSKNEQSNT 248
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Planmine SMEST
Match: SMESG000052500.1 (SMESG000052500.1) HSP 1 Score: 483.411 bits (1243), Expect = 5.050e-175 Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 3 Query: 33 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENAKIPIMFNIEA 734 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENAKIPIMFNIEA Sbjct: 1 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENAKIPIMFNIEA 234
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Planmine SMEST
Match: SMESG000052500.1 (SMESG000052500.1) HSP 1 Score: 462.996 bits (1190), Expect = 4.242e-167 Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 3 Query: 33 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 704 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA Sbjct: 1 MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYILKSTDVHMACEDHNRSRVSAERILRGKSVGIVNIQATYLPIWVLINKSDEHMYLKAKFDEPEIVKIDNVCDYPPTLGKYIRKNYLHNQFNPKILMKPFEKYMDFKSVNEPSEQDKAQIFPPYETVAADGLVIKKPTELPFIYYIKSRQYHGIKFKVYLKEKGKVPTEDQLEENA 224 The following BLAST results are available for this feature:
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of MAP/microtubule affinity-regulating kinase 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006834 ID=SMED30006834|Name=MAP/microtubule affinity-regulating kinase 3|organism=Schmidtea mediterranea sexual|type=transcript|length=896bpback to top protein sequence of SMED30006834-orf-1 >SMED30006834-orf-1 ID=SMED30006834-orf-1|Name=SMED30006834-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=240bp MPIIQHVGKKCVTNYKFLGEICKNLKNFGVGRLVYNASEAALYKEPCYYIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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