G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30006805
Length (bp)1492
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30006805) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30006805) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30006805) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30006805) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006805

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30006805SMESG000079690.1 SMESG000011532.1 dd_Smed_v4_9021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006805SMESG000079690.1 SMESG000011532.1 dd_Smed_v4_9021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30006805SMESG000079690.1 SMESG000011532.1 dd_Smed_v4_9021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30006805SMESG000079690.1 SMESG000011532.1 dd_Smed_v4_9021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A2H1CI12 (7 transmembrane receptor OS=Fasciola hepatica OX=6192 GN=D915_05571 PE=4 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 9.718e-48
Identity = 111/290 (38.28%), Postives = 187/290 (64.48%), Query Frame = 1 
Query:  184 KVKILHFCVAISSTHVVILIMNSLMDDFMGKGLEHWRPE-YRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPL-FVSSHLEFQFNKS-LKC-ALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQY------NGCSLYA-KV--LSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSS 1014
            +  I   C+AIS+   + L  N+++DDFMG+GL +     +  K D  S   C+++EY+ N MY  +S +++VF++DR++T+ KP+ FYSIH R++A  +C+++ L + I+ IPL +V + +  + +++   C  ++++ + K S+  +V  TF++P FI+LILN +I   LW+ K +R +L+P D  +N LEM R+ GHLAL+T FLL   P+V  +L+R +      +  S YA K+  LSR FSS+KDI+Y+++F LY ++L+NFR RF++     LL   T R+++
Sbjct:   42 RFSIYAICLAISNA--ITLTFNTVVDDFMGRGLFYATNHTWYYKLDATSQFGCKLVEYISNSMYFTTSYIIVVFTLDRLLTVRKPITFYSIHHRRWATGICVVIYLLALIVNIPLIYVQTLVADRHSRTNFTCRMIEDHPVAKFSITFEVIFTFTVPFFIVLILNCLIGAALWKLKHERKRLVPADRVRNQLEMGRVTGHLALSTAFLLLYMPMVCLVLIRLHLTLIHVDRHSPYAVKIIDLSRFFSSMKDITYAINFPLYMVYLSNFRRRFLRIFCPCLLRVTTHRQAT 329
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A504YBU0 (Type-1 angiotensin II receptor A OS=Fasciola gigantica OX=46835 GN=FGIG_09800 PE=4 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 1.276e-44
Identity = 108/293 (36.86%), Postives = 178/293 (60.75%), Query Frame = 1 
Query:  184 KVKILHFCVAISSTHVVILIMNSLMDDFMGKGLEHWRPE-YRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLF------VSSHLEFQFNKSLKCALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQY------NGCSLYA-KV--LSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSS 1014
            +  I   C+AIS+   + L  N+++DDFMG+GL +     +  K D  S   C+++EY+ N MY  +S +++VF++DR++T+ KP+ FYSIH R++A  +C+++ L + I+  PL       V  H    F   +   ++++ + K ++  +V  TF++P  I+LILN +I   LW+ K +R +L+P D  +N LEM R+ GHLAL+T FLL   P+V  +L+R +      +  S YA K+  LSR FSS+KDI+Y+++F LY ++L+NFR RF+       L   T R+++
Sbjct:   47 RFSIYAICLAISNA--ITLTFNTVVDDFMGRGLFYATNHTWYYKLDATSQFGCKLVEYIANSMYFTTSYIIVVFTLDRLLTVRKPITFYSIHHRRWATGICVVIYLLALIVNTPLIYVQTLVVDRHSRTNFTCRM---IEDHPVAKFTITFEVIFTFTVPFCIVLILNCLIGAALWKLKHERKRLVPADRVRNQLEMGRVTGHLALSTAFLLLYMPMVCLVLIRLHLTLTHVDRHSPYAVKIIDLSRFFSSMKDITYAINFPLYMVYLSNFRRRFLSIFCPCFLQVTTHRQAT 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: G4VJR3 (Putative peptide (Allatostatin/somatostatin)-like receptor OS=Schistosoma mansoni OX=6183 GN=Smp_041880 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.612e-44
Identity = 110/285 (38.60%), Postives = 174/285 (61.05%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHW-RPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIP-LFVSS-HLEFQFNKSLKC-ALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMR---------QYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSL 1017
            + + ++  +++ LIMN+ +DDF+G+GL +    ++  K DT S   C+ +EY+ N MY  SS L+++FSIDR++TI KP++FYSI+ + +A+  CL+V     I   P LFV +  ++     +  C  +  + I K ++  +  VTF+IP  ++L LNI IC++LW+ K+ R  LLP D  +N +EM R+ GHLAL+T FLL   P+V  +L+R         ++   ++    LSRLFSSVKDI+Y+++F LY IFL NFR  F + +    ++C  V   SL
Sbjct:   52 YSIFLAIANMITLIMNTFIDDFLGRGLYYLTNHQFYFKLDTISKFWCKSVEYLSNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACLLVYFIGIISNSPLLFVQTLMIDISSRTNFTCRMISEHPIAKFTIAFETIVTFTIPFCLVLFLNIFICIELWKLKVHRTALLPADCSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSQTLSHVDRHKPYAMNIIDLSRLFSSVKDITYAVNFFLYLIFLRNFRHGFQRLICT--VYCKKVSSGSL 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183QJJ7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.112e-44
Identity = 112/285 (39.30%), Postives = 176/285 (61.75%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHW-RPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIP-LFVSSHLEFQFNKS-LKC-ALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMR---------QYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSL 1017
            + + ++ T+++ LIMN+ +DDF+G+GL +    +Y  K DT S   C+ +EY+ N MY  SS L+++FSIDR++TI KP++FYSI+ + +A+  C++V     I   P LFV + +    +++   C  +  + I K ++  +  VTF+IP  ++L LNI IC++LW+ KI R  LLP D  +N +EM R+ GHLAL+T FLL   P+V  +L+R         ++   ++    LSRLFSSVKDI+Y+++F LY IFL NFR  F + +    ++C  V   SL
Sbjct:   52 YSIFLAITNMITLIMNTFIDDFLGRGLYYLTNHQYYFKLDTISKFWCKSVEYLSNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSIGIISNSPLLFVQTLINDTSSRTNFTCRMISEHPIAKFTIAFETIVTFTIPFCLVLFLNIFICIELWKLKIHRTTLLPTDCSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSQSLSHVDRHKPYAMNIIDLSRLFSSVKDITYAVNFFLYLIFLRNFRHGFQRLICT--VYCKKVSSRSL 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183L9P8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS523 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.792e-43
Identity = 104/266 (39.10%), Postives = 169/266 (63.53%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHW-RPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIP-LFVSSHLEFQFNKS-LKC-ALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMR---------QYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRF 960
            + + ++  +++ LIMN+ +DDF+G+GL +    ++  K DT S   C+ +EY+ N MY  SS L+++FSIDR++TI KP++FYSI+ + +A+  C++V L   +   P LFV + +  + +++   C  +  + + K ++  +  VTF+IP  ++L LNI IC++LW+ K+ R  LLP D  +N +EM R+ GHLAL+T FLL   P+V  +L+R         ++   ++    LSRLFSSVKDI+Y+++F LY IFL NFR  F
Sbjct:   52 YSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQFYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWAMIACILVYLMGILSNSPLLFVQTLMVDKSSRTNFTCRMISEHPVAKFTITFETIVTFTIPFCLVLFLNIFICIELWKLKVNRTALLPADSSRNCMEMGRVSGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCIIDLSRLFSSVKDITYAVNFFLYLIFLRNFRHGF 317
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000079690.1 (SMESG000079690.1)

HSP 1 Score: 744.962 bits (1922), Expect = 0.000e+0
Identity = 376/378 (99.47%), Postives = 378/378 (100.00%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHWRPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHLEFQFNKSLKCALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSLGLYLDSKLMKHDSNSSRRKYSAIAAALSTCNNHHMFSVLMYKTSGHTSNTRPVYEHCNSYNSSDIHHTPISSQAFKQRCSSVPRQKTVSYASDVKVTTEDETKTLL 1335
            +CVAISSTHVVILIMNSLMDDFMGKGLEHWRPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHLEFQFNKSLKCALKNNTIGK+SVLTKVFVTFSIPTFIILILNIIICLKLWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSLGLYLDSKLMKHDSNSSRRKYSAIAAALSTCNNHHMFSVLMYKTSGHTSNTRPVYEHCNSYNSSDIHHTPISSQAFKQRCSSVPRQKTVSYASDVKVTTEDETKTLL
Sbjct:   56 YCVAISSTHVVILIMNSLMDDFMGKGLEHWRPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHLEFQFNKSLKCALKNNTIGKVSVLTKVFVTFSIPTFIILILNIIICLKLWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYNGCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSLGLYLDSKLMKHDSNSSRRKYSAIAAALSTCNNHHMFSVLMYKTSGHTSNTRPVYEHCNSYNSSDIHHTPISSQAFKQRCSSVPRQKTVSYASDVKVTTEDETKTLL 433

HSP 2 Score: 71.633 bits (174), Expect = 3.254e-13
Identity = 54/56 (96.43%), Postives = 55/56 (98.21%), Query Frame = 2 
Query:   38 MLGKCTSDYPPKWIDYAIGVYRLYAVXXXXXXXXXXXXXXXXXXXXEHKKSRFSIF 205
            MLGKCTSDYPPKWIDYAIGVYRLYAV IIAIFGILGNIIVILVFLLEHKKSRFSI+
Sbjct:    1 MLGKCTSDYPPKWIDYAIGVYRLYAVPIIAIFGILGNIIVILVFLLEHKKSRFSIY 56
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000048976.1 (SMESG000048976.1)

HSP 1 Score: 188.348 bits (477), Expect = 2.541e-54
Identity = 126/338 (37.28%), Postives = 196/338 (57.99%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHWRPEYRI---KFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFS-----CIITIPLFVSSHLEFQFNKSLKCALKN-----NTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYNGCSLYAKV----------LSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSLGLYLDSKLMKHDSNSSRRKYSAIAAALSTCNNHHMFSVLMYKT 1146
            +C+ ++  H+VIL  NS +DDF+G+G+ +    Y     K D  S  +C++MEYVPN  Y ASS ++++FS DRI+TIF+P++F SI+++ +A    ++ ++F+     CI+ I   +          ++KC + +     + I K  +  K+   F IP F++LI+N IIC+KLW+ +  R KLLP D + N LEM+R+ GHLAL++ F+L T PLV+A+++R  +    Y K+          LSRLFSSVKDI+Y+ DF L   FL NFRWRF++Y  +        R S  G   +  + K DS+  +RK+       S  NN  M S L+ ++
Sbjct:   64 YCMLLAMVHIVILFTNSFLDDFLGRGVTY-LSNYTFTSSKIDIKSDFLCKLMEYVPNAAYLASSWIVIIFSCDRIITIFRPLKFMSIYYQLYAWAGSVVTIIFAFLLNICIVIIYEKILDKKHGLAMSNIKCEISSLYKATDFICKYCLYLKLMGAFVIPNFLVLIMNFIICVKLWKIQTTRCKLLPSDSKNNVLEMSRVIGHLALSSCFILMTMPLVIAIILRMNSDRRNYKKLYPEYHDFLKHLSRLFSSVKDINYASDFFLCIFFLPNFRWRFLRYACLFCRRKEWFRLSRWGQIFERNVQKFDSSIVKRKFGTSLHQQSN-NNLEMKSNLLERS 399
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000011885.1 (SMESG000011885.1)

HSP 1 Score: 176.792 bits (447), Expect = 2.324e-50
Identity = 122/296 (41.22%), Postives = 182/296 (61.49%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHWR----PEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYS-IHFRKFAIYLCLIVVLFSCIITIPLFVSSHLEFQFNKS-----LKCALKNNT---IGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMR---------QYNGCSLYAKV--LSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSL 1017
            + + ++ +HV+IL++++ +DDF+G+GL +        +  K DT S   C+ M+Y+P+ MY+ SS + +VFS+DR +TI++P+ FYS +H +K  I+ C++VVL S II +PL V S L  Q NKS     +KC L   +   I +I +  K+  +F +PTFIILILN  I + L++ K  R  L+P D  KN LEMNR+ GHL L++ FLL  FPL++ M +R           N      K+  LSRLFSS+KDISY+ DF +Y +FL NFRW F+ +L + +L C   +   L
Sbjct:   49 YSIFLAGSHVIILVVDTFLDDFIGRGLAYAAYGKCNNHGFKLDTISNASCKTMQYIPDTMYSISSWIFVVFSLDRALTIYRPLLFYSTLHTKKSLIFCCVVVVL-SFIINVPLVVGSGL--QINKSQNVTFVKCDLLTESQFYITRIELYIKMIFSFILPTFIILILNSFIIIILFQIKKSRRDLMPTDRAKNGLEMNRVIGHLGLSSFFLLLAFPLIITMFVRTNWTGRANENLNDKMFLEKLGDLSRLFSSLKDISYAADFYIYVLFLNNFRWYFLDHL-IDILRCCIPKSKRL 340
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000003388.1 (SMESG000003388.1)

HSP 1 Score: 176.022 bits (445), Expect = 5.395e-50
Identity = 117/325 (36.00%), Postives = 191/325 (58.77%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEHWRPEYRI--KFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHL---EFQFNKSLKCAL----KNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLL--------------PGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYNG------CSLYA----KVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHCATVRKSSL--GLYLDSKLMK-HDSNSSR 1068
            +C++++   +V LI+NS MDDF+G+GL H    Y +  K+DT+S   C++MEYVPN M+  +S + ++FSIDR++TI +P++F+S+++ K++I  C+   LF   I IPL +S  L   E +F  +LKC +        +    +   +  +F+IPTFI+LILN +I +KLW+  + RA++               P  ++    E+NR+ GHLA+N VFLL T PLV+ +++R Y+        +LY     + LSRLFSS+KD+++S +F +Y IFL NFR  F  +     ++    R +++   +Y     +K H+S S R
Sbjct:   60 YCISLAFVQLVALIVNSFMDDFLGRGLSHVLGYYLLAFKWDTHSDMSCKMMEYVPNTMFFIASFVTVLFSIDRLLTIHQPLKFHSVYYIKYSIMACVGTFLFGFAINIPLLLSYGLIKQENEFGITLKCTVVATGARKFVADFGLYLVLLGSFTIPTFIVLILNTLISIKLWQLHLNRAEMTKSFDTSDSKSSAINPSLMKNQEKELNRVLGHLAVNVVFLLLTMPLVIVIILRMYSDDNKMYLTNLYLYNSFQHLSRLFSSLKDVNFSCEFFIYMIFLPNFRNGFQNFFQKCFVNKKNFRSNTMINSVYNPKLTVKVHESTSGR 384
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000011884.1 (SMESG000011884.1)

HSP 1 Score: 163.31 bits (412), Expect = 2.447e-45
Identity = 100/275 (36.36%), Postives = 173/275 (62.91%), Query Frame = 1 
Query:  202 FCVAISSTHVVILIMNSLMDDFMGKGLEH---WRPE-YRIKFDTYSLTMCRIMEYVPNCMYTASSGLLLVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHLEFQFNKSL---KC---ALKNNTIGKISVLTKVFVTFSIPTFXXXXXXXXXXXXXWRAKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQY------NGCSLYAKV-----LSRLFSSVKDISYSLDFLLYFIFLTNFRWRFI 963
            + V ++ +++VIL + +L+DDF G+GL      R + Y  K DT S   C++++Y+P  MY  SS L + FS+DR++TI+KP+QF+S    K ++++C++V +   +++IP+ +SS L+   + ++   KC   A  +    +I++  KV V+F +P  I+L++N +I  KLW  K +R  L+  + +++ LEMNRI G+L +++ FLL  FPL++ M++R +             +V     LSRLF+SVKDI+Y++DF +Y +FL NF+W F+
Sbjct:   49 YSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGSLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKVVVSFIVPILIVLVINCVIIGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSQEREQVEILGDLSRLFASVKDINYAVDFYIYLLFLNNFKWNFL 323
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1CI129.718e-4838.287 transmembrane receptor OS=Fasciola hepatica OX=6... [more]
A0A504YBU01.276e-4436.86Type-1 angiotensin II receptor A OS=Fasciola gigan... [more]
G4VJR31.612e-4438.60Putative peptide (Allatostatin/somatostatin)-like ... [more]
A0A183QJJ72.112e-4439.30G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183L9P82.792e-4339.10G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079690.13.254e-1399.47SMESG000079690.1[more]
SMESG000048976.12.541e-5437.28SMESG000048976.1[more]
SMESG000011885.12.324e-5041.22SMESG000011885.1[more]
SMESG000003388.15.395e-5036.00SMESG000003388.1[more]
SMESG000011884.12.447e-4536.36SMESG000011884.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006805 ID=SMED30006805|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1492bp
TTTTTTACATTCTCATTGAATTTCTCTCTGTAACAGAATGCTCGGTAAAT
GTACAAGCGATTACCCGCCGAAATGGATCGATTATGCTATTGGAGTTTAT
CGGTTGTATGCGGTGGCAATTATTGCTATTTTTGGAATTCTTGGAAATAT
TATCGTAATTTTGGTATTTCTATTGGAACATAAAAAGTCAAGATTCTCCA
TTTTTGTGTCGCCATCTCTAGTACTCATGTTGTAATTTTAATAATGAATT
CTCTGATGGATGACTTTATGGGAAAAGGCTTGGAACATTGGCGGCCGGAA
TATAGGATAAAATTTGATACGTATTCACTGACAATGTGCCGAATAATGGA
ATATGTTCCAAACTGTATGTATACGGCGTCTTCCGGTTTGTTATTAGTGT
TTTCTATTGACCGAATTGTAACGATATTTAAGCCAATGCAGTTTTATTCG
ATTCATTTTCGAAAATTCGCCATTTACCTTTGTTTGATTGTTGTTTTGTT
TAGTTGTATAATCACAATTCCGTTATTTGTTTCATCTCATTTGGAATTTC
AATTCAACAAGTCATTGAAATGTGCATTGAAAAATAATACAATCGGTAAA
ATTTCAGTTCTCACAAAGGTATTTGTCACATTTTCCATACCGACTTTTAT
CATTCTCATTCTCAACATCATCATTTGCCTTAAGTTGTGGAGAGCGAAAA
TTCAAAGAGCCAAATTATTGCCCGGAGACCTACAGAAAAATAATCTTGAA
ATGAATCGAATATGCGGACATTTGGCGTTAAATACGGTTTTTCTATTGTT
CACGTTTCCATTGGTTGTTGCGATGCTGATGCGTCAATACAACGGTTGTA
GTCTCTACGCCAAAGTTTTGTCGAGATTATTTTCATCAGTTAAAGATATT
TCATATTCTTTAGATTTTTTATTATATTTTATCTTTTTAACTAATTTTCG
TTGGAGATTTATTAAATATCTCGCTGTACCACTTTTACACTGTGCGACTG
TTAGGAAATCGTCTCTCGGTCTTTACTTAGATTCGAAATTAATGAAACAC
GACAGTAATTCAAGCCGTCGAAAATATAGTGCGATTGCAGCAGCATTATC
CACTTGTAACAACCATCATATGTTCAGTGTTTTAATGTACAAAACAAGTG
GTCATACATCTAACACCCGGCCAGTGTATGAACACTGTAATAGTTATAAT
TCCAGTGATATCCACCATACCCCTATCAGTTCACAAGCCTTCAAACAGAG
ATGTTCATCAGTGCCCCGTCAAAAAACCGTTTCATATGCGTCCGATGTCA
AAGTCACCACAGAAGATGAAACAAAAACTCTTTTATAACAGTAAAATATT
CATGTATATTTATAAAAATACAAAATTCATTCGTATGAAAAATTAAACTA
GATCAAACAATGATTTTGAAGTGATAAAACTTTGTACAAATTTGTGATTT
AAAGTGACTTTTTCATGTCAATAAGGAAACATTTCTTTATTT
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protein sequence of SMED30006805-orf-1

>SMED30006805-orf-1 ID=SMED30006805-orf-1|Name=SMED30006805-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=365bp
MNSLMDDFMGKGLEHWRPEYRIKFDTYSLTMCRIMEYVPNCMYTASSGLL
LVFSIDRIVTIFKPMQFYSIHFRKFAIYLCLIVVLFSCIITIPLFVSSHL
EFQFNKSLKCALKNNTIGKISVLTKVFVTFSIPTFIILILNIIICLKLWR
AKIQRAKLLPGDLQKNNLEMNRICGHLALNTVFLLFTFPLVVAMLMRQYN
GCSLYAKVLSRLFSSVKDISYSLDFLLYFIFLTNFRWRFIKYLAVPLLHC
ATVRKSSLGLYLDSKLMKHDSNSSRRKYSAIAAALSTCNNHHMFSVLMYK
TSGHTSNTRPVYEHCNSYNSSDIHHTPISSQAFKQRCSSVPRQKTVSYAS
DVKVTTEDETKTLL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCDDcd153377tmA_Opsin_Gq_invertebratescoord: 16..46
e-value: 0.00995518
score: 30.3668
NoneNo IPR availableTMHMMTMhelixcoord: 24..46