SMED30006763

Overview
NameSMED30006763
Smed IDSMED30006763
Length (bp)792
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006763 (SMED30006763) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006763 (SMED30006763) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006763 (SMED30006763) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006763 (SMED30006763) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006763

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30006763SMESG000035957.1 SmedASXL_018843SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30006763SMESG000035957.1 dd_Smed_v4_2193_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006763SMESG000035957.1 dd_Smed_v4_2193_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006763SMESG000035957.1 Contig49341newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006763SMESG000035957.1 Contig49341uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30006763SMESG000035957.1 dd_Smed_v4_2193_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30006763SMESG000035957.1 dd_Smed_v4_2193_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30006763SMESG000035957.1 dd_Smed_v4_2193_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30006763 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000574:7978..8769 -3906
Homology
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BLAST of SMED30006763 vs. Planmine SMEST
Match: SMESG000035957.1 (SMESG000035957.1)

HSP 1 Score: 409.453 bits (1051), Expect = 2.845e-146
Identity = 239/242 (98.76%), Postives = 240/242 (99.17%), Query Frame = 3 
Query:   39 MNKISFILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTTLSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENECSATTKTSEILPXXXXXXXXXXXXXXXNADILNVLLIYLREVKYXXXXXXXXXXXXXXXXXXXXXKSCFKRRSHSQIQQRSWKDYYDMKSLISIGV 764
            MNKISFILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPN KTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTTLSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQN QFKVSENECSATTKTSEILPSSRRSTTRVRARANSNADILNVLLIYLREVKYLIIKIFTA+IIFIILVSIFIIKSCFKRRSHSQIQQRSWKDYYDMKSLISIGV
Sbjct:    1 MNKISFILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPNVKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTTLSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNDQFKVSENECSATTKTSEILPSSRRSTTRVRARANSNADILNVLLIYLREVKYLIIKIFTAVIIFIILVSIFIIKSCFKRRSHSQIQQRSWKDYYDMKSLISIGV 242
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BLAST of SMED30006763 vs. Planmine SMEST
Match: SMESG000062659.1 (SMESG000062659.1)

HSP 1 Score: 115.161 bits (287), Expect = 6.654e-31
Identity = 60/146 (41.10%), Postives = 90/146 (61.64%), Query Frame = 3 
Query:   57 ILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTT-LSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENECSATT 491
            I +L +F S   T   W+P+ KL  ANC    IET+CPN     +++I L+T +N QE+ L   MR C+    K++ T  S+ CK LY+V V L+ +NL +C KDC K+ +SS WYLG+  CD+  SN+ Q  ++K ++N C A++
Sbjct:    9 IFLLHLFTSAFAT---WSPDMKLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASS 151
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BLAST of SMED30006763 vs. Planmine SMEST
Match: SMESG000058883.1 (SMESG000058883.1)

HSP 1 Score: 56.225 bits (134), Expect = 8.211e-9
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 3 
Query:  105 WNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTTLSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQ 425
            WNP+ +L    C+E+ +   CP   T   +E +L    +  + V+  T++     +Q + TT SI CK LY+VN+ L    + SC + C     S  W LG + C +
Sbjct:   49 WNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQ-TLQCRINQSQPLTTTFSIQCKNLYRVNISL--SPIFSCTEYCSDIHTSRIWLLGGNTCGE 152

HSP 2 Score: 47.7506 bits (112), Expect = 4.789e-6
Identity = 33/140 (23.57%), Postives = 62/140 (44.29%), Query Frame = 3 
Query:   81 SLSPTPLEWNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKML-TTLSITCKRLYKVNVGL-ISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENECSATTK 494
            SL+    +W+PN K++KA C  + +   CP       +EI+L+ +   +E     + R CK  + + L  + ++ C  +Y  NV +  + +   C   C     S  + LG   C     ++ +   +K   N C  ++K
Sbjct:  327 SLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEE----FSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSK 462
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BLAST of SMED30006763 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.568e-8
Identity = 37/130 (28.46%), Postives = 65/130 (50.00%), Query Frame = 3 
Query:  102 EWNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQK--MLTTLSITCKRLYKVNVGL--ISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENEC 479
            +W+PN KLI A C  + ++ TCP       +E  LL    M +  +S T+ K   P  K  +  +    C+ +Y VN+ +  I+ ++I C + C K   S  W LG+  C +   +  +  +++++EN C
Sbjct:  310 QWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLL---RMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMI-CDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHC 435

HSP 2 Score: 54.299 bits (129), Expect = 3.477e-8
Identity = 43/133 (32.33%), Postives = 62/133 (46.62%), Query Frame = 3 
Query:  105 WNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKML-TTLSITCKRLYKVNVGLIS-ENLISCIKDCPKSFQSSSWYLGK-SKCDQELSNEMQNYQFKVSENECSATTK 494
            W+PN  LI ANC  + IE TCP       +E  L         V SV   KC   N K L  ++ + C  LY+VN+ L     +I+C + C ++  S  W  G   +  Q LSN     ++   +N C A+T+
Sbjct:   21 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQN--VQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDICQNKQFLSNNGSLNKYMTPKNICPASTQ 151
[Close]
BLAST of SMED30006763 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.109e-8
Identity = 37/130 (28.46%), Postives = 65/130 (50.00%), Query Frame = 3 
Query:  102 EWNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQK--MLTTLSITCKRLYKVNVGL--ISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENEC 479
            +W+PN KLI A C  + ++ TCP       +E  LL    M +  +S T+ K   P  K  +  +    C+ +Y VN+ +  I+ ++I C + C K   S  W LG+  C +   +  +  +++++EN C
Sbjct:  314 QWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLL---RMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMI-CDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHC 439

HSP 2 Score: 54.299 bits (129), Expect = 3.333e-8
Identity = 43/133 (32.33%), Postives = 62/133 (46.62%), Query Frame = 3 
Query:  105 WNPNTKLIKANCHELLIETTCPNAKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKML-TTLSITCKRLYKVNVGLIS-ENLISCIKDCPKSFQSSSWYLGK-SKCDQELSNEMQNYQFKVSENECSATTK 494
            W+PN  LI ANC  + IE TCP       +E  L         V SV   KC   N K L  ++ + C  LY+VN+ L     +I+C + C ++  S  W  G   +  Q LSN     ++   +N C A+T+
Sbjct:   25 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQN--VQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDICQNKQFLSNNGSLNKYMTPKNICPASTQ 155
The following BLAST results are available for this feature:
BLAST of SMED30006763 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006763 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035957.12.845e-14698.76SMESG000035957.1[more]
SMESG000062659.16.654e-3141.10SMESG000062659.1[more]
SMESG000058883.18.211e-930.84SMESG000058883.1[more]
SMESG000058905.11.568e-828.46SMESG000058905.1[more]
SMESG000058905.12.109e-828.46SMESG000058905.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006763 ID=SMED30006763|Name=SMED30006763|organism=Schmidtea mediterranea sexual|type=transcript|length=792bp
CAACTTAGAGTTGAATTACAGGAAGTCATAGGAAGACTATGAATAAAATC
AGCTTCATTCTAATGCTTGCAATGTTTGGTTCTCTTTCTCCAACCCCACT
GGAATGGAATCCAAACACGAAACTTATCAAGGCAAATTGTCACGAACTTT
TAATTGAAACGACTTGTCCAAATGCCAAAACCCATGACGTTATGGAAATA
GACTTACTAACAAATATAAATATGCAAGAAGTTGTGTTGAGTGTCACAAT
GAGAAAGTGTAAAACTCCAAATCAGAAAATGTTGACAACTCTTTCAATTA
CCTGTAAAAGACTCTATAAAGTAAATGTAGGCTTGATTTCCGAAAACCTA
ATCAGCTGCATCAAAGACTGCCCAAAATCTTTCCAGAGTTCCAGTTGGTA
TTTGGGTAAATCAAAATGTGATCAAGAGCTATCAAATGAAATGCAAAATT
ATCAATTCAAAGTTTCAGAAAATGAATGTTCCGCTACAACTAAAACATCT
GAAATTCTTCCCTCCTCTAGAAGGTCTACCACAAGAGTACGCGCAAGAGC
TAATTCCAATGCTGATATTTTGAATGTTTTGTTAATATATTTACGTGAGG
TCAAATATCTAATTATTAAAATTTTCACAGCTCTTATAATTTTCATAATT
CTGGTATCAATTTTTATTATTAAAAGTTGTTTTAAGCGAAGATCTCATTC
TCAAATTCAGCAAAGATCATGGAAAGACTATTACGACATGAAATCTTTGA
TAAGTATTGGTGTTTAATTATTTTTAAAAATGTTTTCAATAA
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protein sequence of SMED30006763-orf-1

>SMED30006763-orf-1 ID=SMED30006763-orf-1|Name=SMED30006763-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MNKISFILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPNAKTHD
VMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTTLSITCKRLYKVNVGLI
SENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNYQFKVSENECSAT
TKTSEILPSSRRSTTRVRARANSNADILNVLLIYLREVKYLIIKIFTALI
IFIILVSIFIIKSCFKRRSHSQIQQRSWKDYYDMKSLISIGV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.654
NoneNo IPR availableTMHMMTMhelixcoord: 190..211