SMED30006734
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006734 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SMED30006734 vs. TrEMBL
Match: A0A226D4I7 (Putative AC transposase OS=Folsomia candida OX=158441 GN=Fcan01_25450 PE=4 SV=1) HSP 1 Score: 102.834 bits (255), Expect = 6.044e-31 Identity = 53/114 (46.49%), Postives = 77/114 (67.54%), Query Frame = -1 Query: 267 EWTFQRNHRYINVILHSESK-FWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKK-NTTKYRNRSPNESI 602 EW+ +N RY+NVILH + + FWN+GL+RI+GSL A K + + +K L+ +I+ + D ++V+ K+G+L PT QQLCF H IQL V+DVLYKK N T + S NE++ Sbjct: 191 EWSSIKNRRYMNVILHGKPQEFWNLGLIRIHGSLPAEKCLEMVANKLRLLNLSLENDIVCIITDGSSVMTKIGRLSPTYQQLCFAHGIQLGVLDVLYKKINQTPEADESENENL 304 HSP 2 Score: 60.077 bits (144), Expect = 6.044e-31 Identity = 31/50 (62.00%), Postives = 35/50 (70.00%), Query Frame = -3 Query: 1 ITHNQLGPFIQKVR*IVKIFTRSPTKNETLQNYVNLEFGKEFCLILDSKT 150 I+H L P I KVR IV++F RSP KNE LQ Y+ EFGKE LILD KT Sbjct: 335 ISHT-LRPVIDKVRTIVRMFRRSPLKNEILQTYIKQEFGKELSLILDCKT 383
BLAST of SMED30006734 vs. TrEMBL
Match: A0A4C2AGX5 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_71113_1 PE=4 SV=1) HSP 1 Score: 86.2705 bits (212), Expect = 1.588e-22 Identity = 41/102 (40.20%), Postives = 67/102 (65.69%), Query Frame = -1 Query: 309 EWTFQRNHRYINVILHS----ESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKK 602 EWT N R++NV +H+ + +WN+GL RI GS+ + V V +K +++ L+ K+I+ +T D A+V++KVG+L+ QLC+ H +QL + DV+YKK Sbjct: 73 EWTSGINQRFLNVNVHTVINCQPTYWNIGLARIFGSMSSESCVQVLKEKLSEFDLSLDKDIVGITTDGASVMKKVGRLIEPLLQLCYAHGVQLGITDVIYKK 174 HSP 2 Score: 48.1358 bits (113), Expect = 1.588e-22 Identity = 31/67 (46.27%), Postives = 38/67 (56.72%), Query Frame = -3 Query: 1 NEDGLVMDYEIQRRCTEITHNQLGPFIQKVR*IVKIFTRSPTKNETLQNYVNLEFGKEFCLILDSKT 201 N DGL I R+ T Q+ I K+R VK+F SPTKN+ LQ Y+ EFGK L+LD KT Sbjct: 207 NYDGLYFSLPI-RQVYLATEYQM--IISKLRKCVKLFKNSPTKNDMLQIYIQQEFGKTIQLVLDCKT 270
BLAST of SMED30006734 vs. TrEMBL
Match: A0A4Y2THF1 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_265140_1 PE=4 SV=1) HSP 1 Score: 98.5969 bits (244), Expect = 4.293e-22 Identity = 48/103 (46.60%), Postives = 69/103 (66.99%), Query Frame = -1 Query: 297 EWTFQRNHRYINVILHSESKFWNVGLLRINGSLLAVKWVFV*YDK-WNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTK 602 EWT Q+NHRY+N+ +H + K +N+GL+RI+GS A + + K +G S +I+ +T D A+V+ K GKL+ QQLCF H +QLAVVD+LYKKN + Sbjct: 78 EWTSQKNHRYLNLNVHHKEKRFNLGLIRIHGSCTAEHTISLTKQKRLESFGFDSDTDIIGVTTDGASVMVKFGKLMSCYQQLCFAHGLQLAVVDILYKKNIER 180
BLAST of SMED30006734 vs. TrEMBL
Match: A0A4Y2QRS0 (BED-type domain-containing protein OS=Araneus ventricosus OX=182803 GN=AVEN_88722_1 PE=4 SV=1) HSP 1 Score: 101.293 bits (251), Expect = 4.455e-22 Identity = 48/105 (45.71%), Postives = 70/105 (66.67%), Query Frame = -1 Query: 288 EWTFQRNHRYINVILHSESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTKYRN 602 EWT Q+NHRY+N+ +H + K +N+ L+RI+GS A + + + +GL S +I+ +T D A+V+ KV KL+ QLCF H +QLA+VD+LYKKN K RN Sbjct: 194 EWTSQKNHRYLNLNVHHKEKHFNLSLIRIHGSCTAEHTISLIKTRLESFGLDSDTDIIVVTTDGASVMIKVCKLMSCYHQLCFAHGLQLAMVDILYKKNIEKKRN 298
BLAST of SMED30006734 vs. TrEMBL
Match: A0A4C1SVE1 (AC transposase OS=Eumeta variegata OX=151549 GN=EVAR_3373_1 PE=4 SV=1) HSP 1 Score: 82.8037 bits (203), Expect = 5.853e-22 Identity = 43/108 (39.81%), Postives = 65/108 (60.19%), Query Frame = -1 Query: 279 EWTFQRNHRYINVILHSESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTKYRNRSP 602 EWT N RY+ + +HS KFWN+GL+ I G++ A + + ++ +++ ++ +I ++ D V+ KVG+LV QQLC H I LAV DVLYKK+ Y SP Sbjct: 175 EWTSMSNKRYMALNVHSYKKFWNLGLVPIYGNMTAENCLRILNERLSKFDISLETDIAAIVTDGPNVMIKVGRLVCAEQQLCLAHGINLAVCDVLYKKHN--YLQDSP 280 HSP 2 Score: 49.6766 bits (117), Expect = 5.853e-22 Identity = 31/72 (43.06%), Postives = 44/72 (61.11%), Query Frame = -3 Query: 1 NEDGLVMDYE---IQRRCTEITHNQ-LGPFIQKVR*IVKIFTRSPTKNET-LQNYVNLEFGKEFCLILDSKT 201 +E GL++ + + E+T ++ + I+KVR +V F RSP KNET LQ YV +E GKE L+LD KT Sbjct: 300 SESGLILMSQNDLAREHIPELTQDENIKELIEKVRKVVIYFKRSPLKNETVLQKYVKIEHGKELSLLLDCKT 371
BLAST of SMED30006734 vs. Planmine SMEST
Match: SMESG000036917.1 (SMESG000036917.1) HSP 1 Score: 91.2781 bits (225), Expect = 4.205e-21 Identity = 47/114 (41.23%), Postives = 71/114 (62.28%), Query Frame = -1 Query: 285 GEWTFQRNHRYINVILH-------SESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTKYRNR 605 +WT +RN RYIN+ +H +E+KF ++G++RI+GS+ + K + K + + KNI+ +T D A+V+ KVG LV Q LCF H IQL V+D+LY K+ KY N+ Sbjct: 235 NDWTSKRNRRYININIHYAVEHKITEAKFCSLGMVRIHGSMHSEKCKLIVKTKLQDFNINLEKNIVCMTADGASVILKVGSLVSNYQLLCFAHGIQLVVIDILYTKD--KYSNQ 346
BLAST of SMED30006734 vs. Planmine SMEST
Match: SMESG000036917.1 (SMESG000036917.1) HSP 1 Score: 91.2781 bits (225), Expect = 4.705e-21 Identity = 47/114 (41.23%), Postives = 71/114 (62.28%), Query Frame = -1 Query: 285 GEWTFQRNHRYINVILH-------SESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTKYRNR 605 +WT +RN RYIN+ +H +E+KF ++G++RI+GS+ + K + K + + KNI+ +T D A+V+ KVG LV Q LCF H IQL V+D+LY K+ KY N+ Sbjct: 235 NDWTSKRNRRYININIHYAVEHKITEAKFCSLGMVRIHGSMHSEKCKLIVKTKLQDFNINLEKNIVCMTADGASVILKVGSLVSNYQLLCFAHGIQLVVIDILYTKD--KYSNQ 346
BLAST of SMED30006734 vs. Planmine SMEST
Match: SMESG000036917.1 (SMESG000036917.1) HSP 1 Score: 91.2781 bits (225), Expect = 5.109e-21 Identity = 47/114 (41.23%), Postives = 71/114 (62.28%), Query Frame = -1 Query: 285 GEWTFQRNHRYINVILH-------SESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTKYRNR 605 +WT +RN RYIN+ +H +E+KF ++G++RI+GS+ + K + K + + KNI+ +T D A+V+ KVG LV Q LCF H IQL V+D+LY K+ KY N+ Sbjct: 235 NDWTSKRNRRYININIHYAVEHKITEAKFCSLGMVRIHGSMHSEKCKLIVKTKLQDFNINLEKNIVCMTADGASVILKVGSLVSNYQLLCFAHGIQLVVIDILYTKD--KYSNQ 346
BLAST of SMED30006734 vs. Planmine SMEST
Match: SMESG000065760.1 (SMESG000065760.1) HSP 1 Score: 69.707 bits (169), Expect = 9.444e-14 Identity = 39/99 (39.39%), Postives = 54/99 (54.55%), Query Frame = -1 Query: 306 EWTFQRNHRYINVILHSESKFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKN 602 EWT RY+NV LHS+ + +GL+ I GS A + + K YG+ +NIL+ T D A+V++K +L Q C H I LAVV+ Y KN Sbjct: 154 EWTSCSGKRYLNVTLHSQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGI-KLRNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKN 251
BLAST of SMED30006734 vs. Planmine SMEST
Match: SMESG000018816.1 (SMESG000018816.1) HSP 1 Score: 66.2402 bits (160), Expect = 2.649e-13 Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = -1 Query: 297 EWTFQRNHRYINVILHSES-KFWNVGLLRINGSLLAVKWVFV*YDKWNQYGLTSGKNILSLTNDDAAVLQKVGKLVPTAQQLCFLHTIQLAVVDVLYKKNTTK 602 EWT + RY N+ LH S KF N+GL RINGS + K+ V + Q+G++ I++ T + AAV K G T QLC H+ LAV+D++ K ++ Sbjct: 3 EWTSTKMRRYFNINLHDASGKFNNLGLARINGSFNSEKFQQVVRNHLLQFGISFDIYIVACTTNGAAVNIKFGGERTTEFQLCLAHSAYLAVIDIICDKEDSE 105 The following BLAST results are available for this feature:
BLAST of SMED30006734 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006734 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006734 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30006734 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006734 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006734 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006734 ID=SMED30006734|Name=SMED30006734|organism=Schmidtea mediterranea sexual|type=transcript|length=659bpback to top Annotated Terms
The following terms have been associated with this transcript:
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