DNA-directed RNA polymerases I, II, and III subunit RPABC3

Overview
NameDNA-directed RNA polymerases I, II, and III subunit RPABC3
Smed IDSMED30006696
Length (bp)710
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA-directed RNA polymerases I, II, and III subunit RPABC3 (SMED30006696) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-directed RNA polymerases I, II, and III subunit RPABC3 (SMED30006696) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-directed RNA polymerases I, II, and III subunit RPABC3 (SMED30006696) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-directed RNA polymerases I, II, and III subunit RPABC3 (SMED30006696) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006696

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006696h1SMcG0002216 SmedASXL_009910SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30006696h1SMcG0002216 dd_Smed_v4_3675_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006696h1SMcG0002216 dd_Smed_v4_3675_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30006696h1SMcG0002216 dd_Smed_v4_3675_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30006696h1SMcG0002216 dd_Smed_v4_3675_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Match: POLR2H (RNA polymerase II subunit H [Source:HGNC Symbol;Acc:HGNC:9195])

HSP 1 Score: 140.969 bits (354), Expect = 7.403e-42
Identity = 80/161 (49.69%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Match: POLR2H (RNA polymerase II subunit H [Source:HGNC Symbol;Acc:HGNC:9195])

HSP 1 Score: 137.117 bits (344), Expect = 2.593e-40
Identity = 78/159 (49.06%), Postives = 97/159 (61.01%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKL 587
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKL
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKL 148
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Match: POLR2H (RNA polymerase II subunit H [Source:HGNC Symbol;Acc:HGNC:9195])

HSP 1 Score: 114.39 bits (285), Expect = 7.157e-32
Identity = 70/159 (44.03%), Postives = 84/159 (52.83%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N                                     +DD    S YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYN------------------------------------PTDDRPSSSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 122
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Match: POLR2H (RNA polymerase II subunit H [Source:HGNC Symbol;Acc:HGNC:9195])

HSP 1 Score: 96.2857 bits (238), Expect = 6.488e-25
Identity = 59/129 (45.74%), Postives = 72/129 (55.81%), Query Frame = 3 
Query:  213 MDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS--DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            MDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   ++  +    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    1 MDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 114
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Match: POLR2H (RNA polymerase II subunit H [Source:HGNC Symbol;Acc:HGNC:9195])

HSP 1 Score: 96.2857 bits (238), Expect = 6.488e-25
Identity = 59/129 (45.74%), Postives = 72/129 (55.81%), Query Frame = 3 
Query:  213 MDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS--DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            MDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   ++  +    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    1 MDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 114
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Celegans
Match: rpb-8 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 [Source:UniProtKB/Swiss-Prot;Acc:Q19826])

HSP 1 Score: 130.568 bits (327), Expect = 3.329e-38
Identity = 72/160 (45.00%), Postives = 98/160 (61.25%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS-DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            +F+D+F VK +D DGK+FD+VSR FC+ ESFKM+LI+D+ +QIYPL   DK R  L   LR +       AD+            E   + E  ++  ++Y MYGK+YR+    +  DG  L+ Y SFGGLLM L+G A NL GF+V  ++YLLMKK  F
Sbjct:    5 IFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVLATTLREDGL-----ADE-----------GEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLLMRLKGEAINLHGFEVDMNLYLLMKKTDF 148
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Fly
Match: Rpb8 (gene:FBgn0037121 transcript:FBtr0078432)

HSP 1 Score: 148.673 bits (374), Expect = 3.885e-45
Identity = 82/167 (49.10%), Postives = 103/167 (61.68%), Query Frame = 3 
Query:  111 ATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEI-----EEQKMSNFDYCMYGKIYRIIPGQS-DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
              LFEDIF VK+MD +GK+FD+VSR+ C  ESFKMDLILD+ + +YP+  GDKFR  L   LR +                     C DS E      E  +  +F+Y MYGKIYRI   ++ ++   LS YVSFGGLLM L+G A NL GF+V QH+YLLMK+LAF
Sbjct:    3 GVLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWLYPMELGDKFRLVLATTLREDG--------------------CPDSGEYNPMEHEGTRADSFEYVMYGKIYRIEGDEAHNEASRLSAYVSFGGLLMRLQGDANNLHGFEVDQHMYLLMKRLAF 149
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Zebrafish
Match: polr2h (info polymerase (RNA) II (DNA directed) polypeptide H [Source:ZFIN;Acc:ZDB-GENE-050417-66])

HSP 1 Score: 145.206 bits (365), Expect = 1.290e-43
Identity = 82/161 (50.93%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D DGK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   FDY MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPDGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----PDDGEYNPQDD----------RPSRADQFDYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Xenopus
Match: polr2h (polymerase (RNA) II subunit H [Source:NCBI gene;Acc:548867])

HSP 1 Score: 139.428 bits (350), Expect = 3.024e-41
Identity = 78/161 (48.45%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +G++FD+VSR+ C  ESFKMDLILDV  Q+YP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGRKFDRVSRLHCESESFKMDLILDVNIQVYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYIMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Xenopus
Match: polr2h (polymerase (RNA) II subunit H [Source:NCBI gene;Acc:548867])

HSP 1 Score: 113.235 bits (282), Expect = 2.238e-31
Identity = 68/159 (42.77%), Postives = 84/159 (52.83%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +G++FD+VSR+ C  ESFKMDLILDV  Q+YP+  GDKFR  +   L  + T      DD   N                                     +DD    S YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGRKFDRVSRLHCESESFKMDLILDVNIQVYPVDLGDKFRLVIASTLYEDGT-----LDDGEYN------------------------------------PTDDRPSRSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 122
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Mouse
Match: Polr2h (polymerase (RNA) II (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:2384309])

HSP 1 Score: 140.969 bits (354), Expect = 5.082e-42
Identity = 80/161 (49.69%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Mouse
Match: Polr2h (polymerase (RNA) II (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:2384309])

HSP 1 Score: 112.464 bits (280), Expect = 5.730e-31
Identity = 67/141 (47.52%), Postives = 81/141 (57.45%), Query Frame = 3 
Query:  177 VSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS--DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   ++  +    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:   18 VSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 143
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Mouse
Match: Polr2h (polymerase (RNA) II (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:2384309])

HSP 1 Score: 84.3445 bits (207), Expect = 1.353e-20
Identity = 53/123 (43.09%), Postives = 66/123 (53.66%), Query Frame = 3 
Query:  213 MDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS--DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLL 575
            MDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   ++  +    LS YVS+GGLLM L+G A NL GF+V   +YLL
Sbjct:    1 MDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLL 108
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Mouse
Match: Polr2h (polymerase (RNA) II (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:2384309])

HSP 1 Score: 71.2478 bits (173), Expect = 9.461e-16
Identity = 32/46 (69.57%), Postives = 39/46 (84.78%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPL 254
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPV 50
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Match: sp|Q923G2|RPAB3_MOUSE (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10090 GN=Polr2h PE=1 SV=3)

HSP 1 Score: 140.969 bits (354), Expect = 3.555e-41
Identity = 80/161 (49.69%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Match: sp|P52434|RPAB3_HUMAN (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 140.969 bits (354), Expect = 3.555e-41
Identity = 80/161 (49.69%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILDV  QIYP+  GDKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGT-----LDDGEYNPTDD----------RPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Match: sp|A8XT81|RPAB3_CAEBR (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.120e-38
Identity = 74/160 (46.25%), Postives = 99/160 (61.88%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS-DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            +F+D+F VK +D DGK+FD+VSR FC+ ESFKM+LI+D+ +QIYPL   DKFR  L   LR +       AD+            E   + E  ++  ++Y MYGK+YR+    S  DG  L+ Y SFGGLLM L+G A NL GF+V  ++YLLMKK  F
Sbjct:    5 IFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVLATTLREDGL-----ADE-----------GEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLLMRLKGEAINLHGFEVDMNLYLLMKKTDF 148
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Match: sp|Q19826|RPAB3_CAEEL (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 4.235e-37
Identity = 72/160 (45.00%), Postives = 98/160 (61.25%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQS-DDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            +F+D+F VK +D DGK+FD+VSR FC+ ESFKM+LI+D+ +QIYPL   DK R  L   LR +       AD+            E   + E  ++  ++Y MYGK+YR+    +  DG  L+ Y SFGGLLM L+G A NL GF+V  ++YLLMKK  F
Sbjct:    5 IFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVLATTLREDGL-----ADE-----------GEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLLMRLKGEAINLHGFEVDMNLYLLMKKTDF 148
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Match: sp|O81097|RPB8A_ARATH (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 3.293e-28
Identity = 61/159 (38.36%), Postives = 92/159 (57.86%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIP--GQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKL 587
            LFEDIF V ++D DGK+FDKV+R+     + +M + LDV T++YPLA GDKF   L   L                NLD   +    +   ++     ++Y M+GK+Y+I    G++   E+   YVSFGGLLM+L+G   ++S F++ Q ++LLM+KL
Sbjct:    7 LFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPTL----------------NLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAEL---YVSFGGLLMLLKGDPAHISHFELDQRLFLLMRKL 146
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Match: A0A182Y1H7 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Anopheles stephensi OX=30069 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.169e-44
Identity = 81/161 (50.31%), Postives = 104/161 (64.60%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRII--PGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK+MD +GK+FD+VSR+ C  ESFKMDLILD+ + +YPL  GDKFR  L   L  + T   M+                ++ EIE  +  +F+Y M+GK+YRI     Q++    LS YVSFGGLLM L+G A NL GF++ QH+YLLMKKLAF
Sbjct:    5 LFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWLYPLELGDKFRLVLTTTLHEDGTAASMEY---------------NAAEIEGSRADSFEYVMFGKVYRIEGDDSQTESSNRLSAYVSFGGLLMRLQGDANNLHGFEIDQHMYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Match: A0A0X3PBI0 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS17329 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.251e-44
Identity = 88/163 (53.99%), Postives = 107/163 (65.64%), Query Frame = 3 
Query:  108 MATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQSD-DGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            MA L EDIF VK++DKDGK+FDKVSR+FC  ESFKM LILD+ +QIYPL  GDKFR  L ++L        ++      + DD+    E    +   KM+ FDY  YGKIYRI    S  D   L+ YVSFGGLLM L G A+NL GF+VG+ IYL+MKKLAF
Sbjct:    1 MAALLEDIFDVKDIDKDGKKFDKVSRLFCESESFKMGLILDINSQIYPLTKGDKFRLVLTKSL--------LNDAVADDDDDDEDAAEETDGRLTNSKMAEFDYVCYGKIYRIESHDSSVDSSRLACYVSFGGLLMQLTGDASNLQGFRVGKRIYLMMKKLAF 155
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Match: A0A182S8D0 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Anopheles maculatus OX=74869 PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.468e-44
Identity = 81/161 (50.31%), Postives = 104/161 (64.60%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRII--PGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK+MD +GK+FD+VSR+ C  ESFKMDLILD+ + +YPL  GDKFR  L   L  + T   M+                ++ EIE  +  +F+Y M+GK+YRI     Q++    LS YVSFGGLLM L+G A NL GF++ QH+YLLMKKLAF
Sbjct:    5 LFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWLYPLELGDKFRLVLATTLHEDGTAASMEY---------------NAAEIEGSRADSFEYVMFGKVYRIEGDDSQTESSNRLSAYVSFGGLLMRLQGDANNLHGFEIDQHMYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Match: A0A3P7NJT3 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS18296 PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.606e-44
Identity = 88/163 (53.99%), Postives = 106/163 (65.03%), Query Frame = 3 
Query:  108 MATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQSD-DGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            MA L EDIF VK++DKDGK+FDKVSR+FC  ESFKM LILD+ +QIYPL  GDKFR  L + L        ++      + DD+    E    +   KM+ FDY  YGKIYRI    S  D   L+ YVSFGGLLM L G A+NL GF+VG+ IYL+MKKLAF
Sbjct:    1 MAALLEDIFDVKDIDKDGKKFDKVSRLFCESESFKMGLILDINSQIYPLTKGDKFRLVLTKTL--------LNDAVGDDDDDDEDAAEETDGRLTNSKMAEFDYVCYGKIYRIESHDSSVDSSRLACYVSFGGLLMQLTGDASNLQGFRVGKRIYLMMKKLAF 155
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Match: U6JC54 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Echinococcus granulosus OX=6210 GN=EGR_04064 PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 3.857e-44
Identity = 90/164 (54.88%), Postives = 110/164 (67.07%), Query Frame = 3 
Query:  108 MATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            M  L EDIF VK++DKDGK+FDKVSRIF   ESFKM LILD+ +QIYPL  G+KFR  L ++L  + + +  D DD            E    ++  KM+ FDY  YGKIYR I GQ  SDD   L+ YVSFGGLLM L G A+NL GF+VG+H+YL+MKKLAF
Sbjct:    1 MTALLEDIFDVKDVDKDGKKFDKVSRIFGESESFKMGLILDIHSQIYPLNKGEKFRLVLTKSLLNDVSNVADDDDDDE------DAAEELDGRLDNSKMAEFDYVCYGKIYR-IEGQDGSDDTNRLACYVSFGGLLMQLTGDASNLQGFRVGKHLYLMMKKLAF 157
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Cavefish
Match: polr2h (RNA polymerase II subunit H [Source:NCBI gene;Acc:103046918])

HSP 1 Score: 145.976 bits (367), Expect = 4.349e-44
Identity = 82/161 (50.93%), Postives = 100/161 (62.11%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D DGK+FD+VSR+ C  ESFKMDLILDV TQIYP+  GDKFR  +   L  + T      DD   N  DD             +   FDY MYGK+Y+I   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPDGKKFDRVSRLHCESESFKMDLILDVNTQIYPVDLGDKFRLVIASTLYEDGT-----PDDGEYNPQDD----------RPSRADQFDYVMYGKVYKIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009100.1 (pep scaffold:Pmarinus_7.0:GL482486:12996:17534:1 gene:ENSPMAG00000008230.1 transcript:ENSPMAT00000009100.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 142.895 bits (359), Expect = 1.526e-43
Identity = 80/161 (49.69%), Postives = 99/161 (61.49%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK+MD +GK+FD+VSR+ C  ESFKMDLILDV TQIYP+   DKFR  +   L  + T      DD   N  DD             +   F+Y MYGK+YRI   +  ++    L+ YVSFGGLLM L+G A NL GF+V   +YLL+KKLAF
Sbjct:    5 LFEDIFDVKDMDPEGKKFDRVSRLHCESESFKMDLILDVNTQIYPVDLADKFRLVVANTLYEDGT-----PDDGEYNPSDD----------RPSRAEQFEYVMYGKVYRIEGDETSTEAATRLAAYVSFGGLLMRLQGDANNLHGFEVDSRVYLLIKKLAF 150
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Yeast
Match: RPB8 (RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III [Source:SGD;Acc:S000005750])

HSP 1 Score: 67.781 bits (164), Expect = 6.882e-15
Identity = 48/158 (30.38%), Postives = 79/158 (50.00%), Query Frame = 3 
Query:  114 TLFEDIFGVKEMDKDGKRFDKVSRIFCNGESF-KMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKK 584
            TLF+DIF V E+D    R++KV RI     +  +  L LD+  +++P+A  D     +  +L +E T  +  +   TR+           +  +     ++DY MYG  Y+      D   +++ Y SFGGLLM L G   NL+  K  ++ YLL+++
Sbjct:    4 TLFDDIFQVSEVDP--GRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLNLEDTPANDSS--ATRSW-------RPPQAGDRSLADDYDYVMYGTAYKFEEVSKD---LIAVYYSFGGLLMRLEGNYRNLNNLK-QENAYLLIRR 146
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Nematostella
Match: EDO40011 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8M4])

HSP 1 Score: 141.739 bits (356), Expect = 7.559e-43
Identity = 76/162 (46.91%), Postives = 100/162 (61.73%), Query Frame = 3 
Query:  111 ATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ-SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
              LFEDIF VK++D +GK+FD+VSR+ C  ESFKMDLILD+ +Q+YP+  GDKFR  L   L+ + T             DD+    + +    +Q    F+Y MYGK+YRI   +  D    L+ YVS+GGLLM L+G A NL G +   H+YLLMKKLAF
Sbjct:    3 GVLFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDINSQVYPVELGDKFRLVLATTLKEDGTP------------DDEFNPVDTTPSRADQ----FEYVMYGKVYRIEGDEGPDPASRLAAYVSYGGLLMRLQGDANNLHGIEADDHVYLLMKKLAF 148
[Close]
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Medaka
Match: polr2h (RNA polymerase II subunit H [Source:NCBI gene;Acc:101159426])

HSP 1 Score: 147.132 bits (370), Expect = 1.701e-44
Identity = 82/161 (50.93%), Postives = 101/161 (62.73%), Query Frame = 3 
Query:  117 LFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLAPGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMSNFDYCMYGKIYRIIPGQ--SDDGEILSTYVSFGGLLMMLRGAATNLSGFKVGQHIYLLMKKLAF 593
            LFEDIF VK++D DGK+FD+VSR+ C  ESFKMDLILDV TQIYP+  GDKFR  +   L  + T      DD   N  DD             + + FDY MYGK+Y+I   +  ++    LS YVS+GGLLM L+G A NL GF+V   +YLLMKKLAF
Sbjct:    5 LFEDIFDVKDIDPDGKKFDRVSRLHCESESFKMDLILDVNTQIYPVDLGDKFRLVIASTLYEDGT-----PDDGEYNPQDD----------RPSRAAQFDYVMYGKVYKIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF 150
The following BLAST results are available for this feature:
BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
POLR2H7.403e-4249.69RNA polymerase II subunit H [Source:HGNC Symbol;Ac... [more]
POLR2H2.593e-4049.06RNA polymerase II subunit H [Source:HGNC Symbol;Ac... [more]
POLR2H7.157e-3244.03RNA polymerase II subunit H [Source:HGNC Symbol;Ac... [more]
POLR2H6.488e-2545.74RNA polymerase II subunit H [Source:HGNC Symbol;Ac... [more]
POLR2H6.488e-2545.74RNA polymerase II subunit H [Source:HGNC Symbol;Ac... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpb-83.329e-3845.00Probable DNA-directed RNA polymerases I, II, and I... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rpb83.885e-4549.10gene:FBgn0037121 transcript:FBtr0078432[more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr2h1.290e-4350.93info polymerase (RNA) II (DNA directed) polypeptid... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
polr2h3.024e-4148.45polymerase (RNA) II subunit H [Source:NCBI gene;Ac... [more]
polr2h2.238e-3142.77polymerase (RNA) II subunit H [Source:NCBI gene;Ac... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Polr2h5.082e-4249.69polymerase (RNA) II (DNA directed) polypeptide H [... [more]
Polr2h5.730e-3147.52polymerase (RNA) II (DNA directed) polypeptide H [... [more]
Polr2h1.353e-2043.09polymerase (RNA) II (DNA directed) polypeptide H [... [more]
Polr2h9.461e-1669.57polymerase (RNA) II (DNA directed) polypeptide H [... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q923G2|RPAB3_MOUSE3.555e-4149.69DNA-directed RNA polymerases I, II, and III subuni... [more]
sp|P52434|RPAB3_HUMAN3.555e-4149.69DNA-directed RNA polymerases I, II, and III subuni... [more]
sp|A8XT81|RPAB3_CAEBR1.120e-3846.25Probable DNA-directed RNA polymerases I, II, and I... [more]
sp|Q19826|RPAB3_CAEEL4.235e-3745.00Probable DNA-directed RNA polymerases I, II, and I... [more]
sp|O81097|RPB8A_ARATH3.293e-2838.36DNA-directed RNA polymerases II and V subunit 8A O... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A182Y1H71.169e-4450.31DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A0X3PBI01.251e-4453.99DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A182S8D01.468e-4450.31DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A3P7NJT31.606e-4453.99DNA-directed RNA polymerases I, II, and III subuni... [more]
U6JC543.857e-4454.88DNA-directed RNA polymerases I, II, and III subuni... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr2h4.349e-4450.93RNA polymerase II subunit H [Source:NCBI gene;Acc:... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000009100.11.526e-4349.69pep scaffold:Pmarinus_7.0:GL482486:12996:17534:1 g... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RPB86.882e-1530.38RNA polymerase subunit ABC14.5; common to RNA poly... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO400117.559e-4346.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polr2h1.701e-4450.93RNA polymerase II subunit H [Source:NCBI gene;Acc:... [more]
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BLAST of DNA-directed RNA polymerases I, II, and III subunit RPABC3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006696 ID=SMED30006696|Name=DNA-directed RNA polymerases I, II, and III subunit RPABC3|organism=Schmidtea mediterranea sexual|type=transcript|length=710bp
ATTTATTCCACATTTTAGGGTCAAATCATAATTTGTTTAAAATACATTTG
CAGTAAAATATCTTCAAACGTGAGAATTTTTATCTGATTAATTTTTTATT
TAATAATATGGCTACATTATTTGAAGATATTTTTGGAGTTAAAGAAATGG
ATAAAGATGGGAAGCGATTTGATAAAGTGTCCAGGATATTTTGCAACGGT
GAATCCTTTAAGATGGATTTAATTTTAGATGTTCAAACTCAGATATATCC
TTTAGCTCCAGGTGATAAATTTCGATTTTGTTTAATAAGAAATCTACGGA
TGGAGTCTACGGGAATACACATGGATGCTGATGATGTAACTAGAAATTTA
GACGATGATGTTGAAATGTGTGAAGATTCTGAAGAAATAGAGGAGCAAAA
AATGAGCAATTTTGATTATTGCATGTACGGAAAAATTTATCGAATTATAC
CTGGTCAATCTGACGACGGTGAAATTTTATCAACTTACGTTTCATTTGGT
GGATTATTAATGATGCTAAGAGGTGCTGCTACCAATTTAAGTGGATTCAA
AGTTGGACAGCATATTTATTTATTAATGAAAAAATTAGCTTTTTAATAAA
ATGCAGTATTTTTTATTTTGTGGATAATGCTTCATAATCTTCACCCGAGT
TTTCACAGAAGAACTGCATTTTTATTTCACAAATTTTGATGACTTCATTG
TCGAAGCTAT
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protein sequence of SMED30006696-orf-1

>SMED30006696-orf-1 ID=SMED30006696-orf-1|Name=SMED30006696-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MATLFEDIFGVKEMDKDGKRFDKVSRIFCNGESFKMDLILDVQTQIYPLA
PGDKFRFCLIRNLRMESTGIHMDADDVTRNLDDDVEMCEDSEEIEEQKMS
NFDYCMYGKIYRIIPGQSDDGEILSTYVSFGGLLMMLRGAATNLSGFKVG
QHIYLLMKKLAF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005665DNA-directed RNA polymerase II, core complex
GO:0005666DNA-directed RNA polymerase III complex
GO:0005736DNA-directed RNA polymerase I complex
Vocabulary: biological process
TermDefinition
GO:0006366transcription from RNA polymerase II promoter
GO:0006383transcription from RNA polymerase III promoter
GO:0006351transcription, DNA-templated
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000429neoblast
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR005570RNA_pol_Rpb8
IPR012340NA-bd_OB-fold
Vocabulary: molecular function
TermDefinition
GO:0003899DNA-directed 5'-3' RNA polymerase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 1..159
e-value: 4.6E-49
score: 179.0
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..161
e-value: 3.4E-45
score: 151.3
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 6..159
e-value: 1.6E-44
score: 151.4
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..160
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 5..162
e-value: 7.5E-47
score: 160.8
NoneNo IPR availablePANTHERPTHR10917:SF0DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..160
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 3..159