Alpha-1,4 glucan phosphorylase

Overview
NameAlpha-1,4 glucan phosphorylase
Smed IDSMED30006686
Length (bp)627
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Alpha-1,4 glucan phosphorylase (SMED30006686) t-SNE clustered cells

Violin plots show distribution of expression levels for Alpha-1,4 glucan phosphorylase (SMED30006686) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006686

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30006686h1SMcG0002712 Contig16039newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30006686h1SMcG0002712 Contig16039uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
testisSMED30006686h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30006686h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30006686h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGM (glycogen phosphorylase, muscle associated [Source:HGNC Symbol;Acc:HGNC:9726])

HSP 1 Score: 195.282 bits (495), Expect = 1.089e-57
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV KL+  GY+ +++ +  PEL+QV+EQ+ + +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  596 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 750
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGM (glycogen phosphorylase, muscle associated [Source:HGNC Symbol;Acc:HGNC:9726])

HSP 1 Score: 194.512 bits (493), Expect = 4.700e-57
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV KL+  GY+ +++ +  PEL+QV+EQ+ + +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGB (glycogen phosphorylase B [Source:HGNC Symbol;Acc:HGNC:9723])

HSP 1 Score: 189.504 bits (480), Expect = 3.086e-55
Identity = 86/155 (55.48%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE G +N+FIFG+R +DV  L+  GY+ +++ +  PELKQ ++QI + +FS   P  F DI N + +HDRF + AD++ YM+ Q  V++ Y+N   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGL (glycogen phosphorylase L [Source:HGNC Symbol;Acc:HGNC:9725])

HSP 1 Score: 186.422 bits (472), Expect = 2.651e-54
Identity = 87/152 (57.24%), Postives = 112/152 (73.68%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPL 516
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV  L+  GY+ K++ E  PELK V++QI N +FS   P +F DI N ++YHDRF + AD++ Y+K QD V++ Y N   W   ++ NIA SGKFSSDRTI EYA+ IW VEP+++ +
Sbjct:  650 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKI 801
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGL (glycogen phosphorylase L [Source:HGNC Symbol;Acc:HGNC:9725])

HSP 1 Score: 186.422 bits (472), Expect = 3.598e-54
Identity = 87/152 (57.24%), Postives = 112/152 (73.68%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPL 516
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV  L+  GY+ K++ E  PELK V++QI N +FS   P +F DI N ++YHDRF + AD++ Y+K QD V++ Y N   W   ++ NIA SGKFSSDRTI EYA+ IW VEP+++ +
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKI 835
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 182.185 bits (461), Expect = 1.811e-57
Identity = 79/156 (50.64%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGT+DGANVEMAEE+G++N+FIFGM  ++V  L   GY  ++F+++ P LKQ++EQI+   F+ ++P    D+ N + YHDRFM+CADF  +++AQD V+  +++Q  W    + NIA +GKFS+DRTI+EYA+EIWG++     LP P+
Sbjct:   86 LNGALTIGTLDGANVEMAEEMGDENIFIFGMNVEEVEALTKRGYSSQEFIDKSPMLKQIIEQIEGGMFTPEDPDQLKDLSNMLRYHDRFMVCADFDAFIEAQDKVSATFRDQEKWSRMALYNIASTGKFSTDRTITEYAREIWGIDQFESSLPAPY 241
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 180.259 bits (456), Expect = 2.974e-53
Identity = 79/156 (50.64%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGT+DGANVEMAEE+G++N+FIFGM  ++V  L   GY  ++F+++ P LKQ++EQI+   F+ ++P    D+ N + YHDRFM+CADF  +++AQD V+  +++Q  W    + NIA +GKFS+DRTI+EYA+EIWG++     LP P+
Sbjct:  463 LNGALTIGTLDGANVEMAEEMGDENIFIFGMNVEEVEALTKRGYSSQEFIDKSPMLKQIIEQIEGGMFTPEDPDQLKDLSNMLRYHDRFMVCADFDAFIEAQDKVSATFRDQEKWSRMALYNIASTGKFSTDRTITEYAREIWGIDQFESSLPAPY 618
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 179.874 bits (455), Expect = 2.800e-52
Identity = 79/156 (50.64%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGT+DGANVEMAEE+G++N+FIFGM  ++V  L   GY  ++F+++ P LKQ++EQI+   F+ ++P    D+ N + YHDRFM+CADF  +++AQD V+  +++Q  W    + NIA +GKFS+DRTI+EYA+EIWG++     LP P+
Sbjct:  685 LNGALTIGTLDGANVEMAEEMGDENIFIFGMNVEEVEALTKRGYSSQEFIDKSPMLKQIIEQIEGGMFTPEDPDQLKDLSNMLRYHDRFMVCADFDAFIEAQDKVSATFRDQEKWSRMALYNIASTGKFSTDRTITEYAREIWGIDQFESSLPAPY 840
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 179.874 bits (455), Expect = 3.375e-52
Identity = 79/156 (50.64%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGT+DGANVEMAEE+G++N+FIFGM  ++V  L   GY  ++F+++ P LKQ++EQI+   F+ ++P    D+ N + YHDRFM+CADF  +++AQD V+  +++Q  W    + NIA +GKFS+DRTI+EYA+EIWG++     LP P+
Sbjct:  721 LNGALTIGTLDGANVEMAEEMGDENIFIFGMNVEEVEALTKRGYSSQEFIDKSPMLKQIIEQIEGGMFTPEDPDQLKDLSNMLRYHDRFMVCADFDAFIEAQDKVSATFRDQEKWSRMALYNIASTGKFSTDRTITEYAREIWGIDQFESSLPAPY 876
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Match: GlyP (gene:FBgn0004507 transcript:FBtr0100485)

HSP 1 Score: 174.096 bits (440), Expect = 4.420e-50
Identity = 87/155 (56.13%), Postives = 110/155 (70.97%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGT+DGANVEMAEE+G DN+FIFGM   +V  L   GY+   +    PE+KQV++QI+  +FS  NP  F +I + +  +D + L AD+  Y+KAQDLV+K Y+NQ  W    + NIA SGKFSSDRTI+EYA+EIWGVEPT   LP P
Sbjct:  684 LNGALTIGTLDGANVEMAEEMGLDNIFIFGMTVDEVEALKKKGYNAYDYYNANPEVKQVIDQIQGGFFSPGNPNEFKNIADILLKYDHYYLLADYDAYIKAQDLVSKTYQNQAKWLEMSINNIASSGKFSSDRTIAEYAREIWGVEPTWEKLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Match: GlyP (gene:FBgn0004507 transcript:FBtr0077828)

HSP 1 Score: 174.096 bits (440), Expect = 4.420e-50
Identity = 87/155 (56.13%), Postives = 110/155 (70.97%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGT+DGANVEMAEE+G DN+FIFGM   +V  L   GY+   +    PE+KQV++QI+  +FS  NP  F +I + +  +D + L AD+  Y+KAQDLV+K Y+NQ  W    + NIA SGKFSSDRTI+EYA+EIWGVEPT   LP P
Sbjct:  684 LNGALTIGTLDGANVEMAEEMGLDNIFIFGMTVDEVEALKKKGYNAYDYYNANPEVKQVIDQIQGGFFSPGNPNEFKNIADILLKYDHYYLLADYDAYIKAQDLVSKTYQNQAKWLEMSINNIASSGKFSSDRTIAEYAREIWGVEPTWEKLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygl (phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZDB-GENE-041205-1])

HSP 1 Score: 197.208 bits (500), Expect = 3.422e-58
Identity = 91/155 (58.71%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +L+  GY+  ++ E   ELK+ + QIK+ YFS   P +F+DI N +++HDRF + AD++ Y+K QD V+  YK+Q  W   ++ NIA SGKFSSDRTI+EYA EIWGVEPT++ +PPP
Sbjct:  709 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVNDVAELDKKGYNALQYYENLSELKRAINQIKSGYFSPKQPEMFSDIINMLFHHDRFKVFADYESYVKCQDRVSALYKDQREWTRVVIKNIAASGKFSSDRTITEYATEIWGVEPTDLKIPPP 863
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygl (phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZDB-GENE-041205-1])

HSP 1 Score: 197.208 bits (500), Expect = 3.556e-58
Identity = 91/155 (58.71%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +L+  GY+  ++ E   ELK+ + QIK+ YFS   P +F+DI N +++HDRF + AD++ Y+K QD V+  YK+Q  W   ++ NIA SGKFSSDRTI+EYA EIWGVEPT++ +PPP
Sbjct:  683 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVNDVAELDKKGYNALQYYENLSELKRAINQIKSGYFSPKQPEMFSDIINMLFHHDRFKVFADYESYVKCQDRVSALYKDQREWTRVVIKNIAASGKFSSDRTITEYATEIWGVEPTDLKIPPP 837
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygb (phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZDB-GENE-040928-2])

HSP 1 Score: 193.356 bits (490), Expect = 6.732e-57
Identity = 89/155 (57.42%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  ++  GY+ +++ E  PELK V++QI + +FS   P +F D+ N +  HDRF + AD++ Y+  QD VN+ YKN   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVQDVEDMDKKGYNAREYYERLPELKLVMDQISSGFFSPKEPELFKDVVNMLMDHDRFKVFADYESYISCQDKVNELYKNPKEWTKKVIRNIAASGKFSSDRTIAEYAREIWGVEPSDVKIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygb (phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZDB-GENE-040928-2])

HSP 1 Score: 193.356 bits (490), Expect = 7.233e-57
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  ++  GY+ +++ E  PELK V++QI   +FS   P +F D+ N +  HDRF + AD++ Y+  QD VN+ YKN   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  677 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVQDVEDMDKKGYNAREYYERLPELKLVMDQISTGFFSPKEPELFKDVVNMLMDHDRFKVFADYESYISCQDKVNELYKNPKEWTKKVIRNIAASGKFSSDRTIAEYAREIWGVEPSDVKIPPP 831
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygb (phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZDB-GENE-040928-2])

HSP 1 Score: 193.356 bits (490), Expect = 7.750e-57
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  ++  GY+ +++ E  PELK V++QI   +FS   P +F D+ N +  HDRF + AD++ Y+  QD VN+ YKN   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVQDVEDMDKKGYNAREYYERLPELKLVMDQISTGFFSPKEPELFKDVVNMLMDHDRFKVFADYESYISCQDKVNELYKNPKEWTKKVIRNIAASGKFSSDRTIAEYAREIWGVEPSDVKIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: PYGM (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:100491051])

HSP 1 Score: 198.364 bits (503), Expect = 1.049e-58
Identity = 90/155 (58.06%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +++  GY+ + + E  PEL+QV++Q+ + YFS   P +F D+ N +  HDRF + AD++DY+K+Q+ VN  YKN   W  K++ NIA SGKFSSDRTI++YA+EIWGVEP  M +PPP
Sbjct:  625 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVERMDREGYNARAYYERIPELRQVIDQLNSGYFSPKQPDLFKDVVNMLMNHDRFKVFADYEDYIKSQEKVNALYKNPKEWTRKVIHNIAGSGKFSSDRTIAQYAREIWGVEPGTMKIPPP 779
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: PYGM (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:100491051])

HSP 1 Score: 197.978 bits (502), Expect = 1.511e-58
Identity = 90/155 (58.06%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +++  GY+ + + E  PEL+QV++Q+ + YFS   P +F D+ N +  HDRF + AD++DY+K+Q+ VN  YKN   W  K++ NIA SGKFSSDRTI++YA+EIWGVEP  M +PPP
Sbjct:  648 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVERMDREGYNARAYYERIPELRQVIDQLNSGYFSPKQPDLFKDVVNMLMNHDRFKVFADYEDYIKSQEKVNALYKNPKEWTRKVIHNIAGSGKFSSDRTIAQYAREIWGVEPGTMKIPPP 802
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: PYGM (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:100491051])

HSP 1 Score: 197.978 bits (502), Expect = 1.932e-58
Identity = 90/155 (58.06%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +++  GY+ + + E  PEL+QV++Q+ + YFS   P +F D+ N +  HDRF + AD++DY+K+Q+ VN  YKN   W  K++ NIA SGKFSSDRTI++YA+EIWGVEP  M +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVERMDREGYNARAYYERIPELRQVIDQLNSGYFSPKQPDLFKDVVNMLMNHDRFKVFADYEDYIKSQEKVNALYKNPKEWTRKVIHNIAGSGKFSSDRTIAQYAREIWGVEPGTMKIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: PYGM (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:100491051])

HSP 1 Score: 197.978 bits (502), Expect = 1.942e-58
Identity = 90/155 (58.06%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +++  GY+ + + E  PEL+QV++Q+ + YFS   P +F D+ N +  HDRF + AD++DY+K+Q+ VN  YKN   W  K++ NIA SGKFSSDRTI++YA+EIWGVEP  M +PPP
Sbjct:  685 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVERMDREGYNARAYYERIPELRQVIDQLNSGYFSPKQPDLFKDVVNMLMNHDRFKVFADYEDYIKSQEKVNALYKNPKEWTRKVIHNIAGSGKFSSDRTIAQYAREIWGVEPGTMKIPPP 839
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: PYGM (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:100491051])

HSP 1 Score: 197.978 bits (502), Expect = 1.971e-58
Identity = 90/155 (58.06%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV +++  GY+ + + E  PEL+QV++Q+ + YFS   P +F D+ N +  HDRF + AD++DY+K+Q+ VN  YKN   W  K++ NIA SGKFSSDRTI++YA+EIWGVEP  M +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVERMDREGYNARAYYERIPELRQVIDQLNSGYFSPKQPDLFKDVVNMLMNHDRFKVFADYEDYIKSQEKVNALYKNPKEWTRKVIHNIAGSGKFSSDRTIAQYAREIWGVEPGTMKIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygb (brain glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97828])

HSP 1 Score: 196.052 bits (497), Expect = 7.324e-58
Identity = 88/155 (56.77%), Postives = 119/155 (76.77%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  L+  GY+ ++F E  PEL+Q ++QI + +FS  +P  F D+ N + YHDRF + AD++ Y++ Q  V++ Y+N   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVEALDQKGYNAREFYERLPELRQAVDQISSGFFSPKDPDCFKDVVNMLMYHDRFKVFADYEAYIQCQAQVDRLYRNSKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 193.741 bits (491), Expect = 2.574e-57
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV +L+  GY+ +++ +  PEL+Q++EQ+ + +FS   P +F DI N + +HDRF + AD+++Y+K QD V++ YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  596 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 750
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 193.741 bits (491), Expect = 2.574e-57
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV +L+  GY+ +++ +  PEL+Q++EQ+ + +FS   P +F DI N + +HDRF + AD+++Y+K QD V++ YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  596 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 750
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 193.356 bits (490), Expect = 7.664e-57
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV +L+  GY+ +++ +  PEL+Q++EQ+ + +FS   P +F DI N + +HDRF + AD+++Y+K QD V++ YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 193.356 bits (490), Expect = 7.664e-57
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV +L+  GY+ +++ +  PEL+Q++EQ+ + +FS   P +F DI N + +HDRF + AD+++Y+K QD V++ YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|Q8CI94|PYGB_MOUSE (Glycogen phosphorylase, brain form OS=Mus musculus OX=10090 GN=Pygb PE=1 SV=3)

HSP 1 Score: 196.052 bits (497), Expect = 5.123e-57
Identity = 88/155 (56.77%), Postives = 119/155 (76.77%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  L+  GY+ ++F E  PEL+Q ++QI + +FS  +P  F D+ N + YHDRF + AD++ Y++ Q  V++ Y+N   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVEALDQKGYNAREFYERLPELRQAVDQISSGFFSPKDPDCFKDVVNMLMYHDRFKVFADYEAYIQCQAQVDRLYRNSKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|Q8HXW4|PYGM_MACFA (Glycogen phosphorylase, muscle form OS=Macaca fascicularis OX=9541 GN=PYGM PE=2 SV=3)

HSP 1 Score: 195.282 bits (495), Expect = 1.050e-56
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV KL+  GY+ +++ +  PEL+QV+EQ+ + +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P11217|PYGM_HUMAN (Glycogen phosphorylase, muscle form OS=Homo sapiens OX=9606 GN=PYGM PE=1 SV=6)

HSP 1 Score: 194.512 bits (493), Expect = 2.257e-56
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N FIFGMR +DV KL+  GY+ +++ +  PEL+QV+EQ+ + +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YKN   W   ++ NIA SGKFSSDRTI++YA+EIWGVEP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P09812|PYGM_RAT (Glycogen phosphorylase, muscle form OS=Rattus norvegicus OX=10116 GN=Pygm PE=1 SV=5)

HSP 1 Score: 194.126 bits (492), Expect = 2.705e-56
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GEDN FIFGMR +DV +L+  GY+ +++ +  PEL+Q++EQ+ + +FS   P +F DI N + +HDRF + AD+++Y+K QD V++ YKN   W   ++ NIA SGKFSSDRTI++YA+EIWG+EP+   LP P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEDNFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMVMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGLEPSRQRLPAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P53534|PYGB_RAT (Glycogen phosphorylase, brain form (Fragment) OS=Rattus norvegicus OX=10116 GN=Pygb PE=1 SV=3)

HSP 1 Score: 193.356 bits (490), Expect = 4.714e-56
Identity = 88/155 (56.77%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  L+  GY+ ++F E  PEL+Q ++QI + +FS  +P  F D+ N + YHDRF + AD++ Y++ Q  V+  Y+N   W  K++ NIA SGKFSSDRTI+EYA+EIWGVEP+++ +PPP
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVEALDQKGYNAQEFYERLPELRQAVDQISSGFFSPKDPDCFKDVVNMLMYHDRFKVFADYEAYIQCQAQVDHLYRNPKDWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A1S8WR11 (Alpha-1,4 glucan phosphorylase (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_07231 PE=3 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 4.766e-63
Identity = 94/156 (60.26%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGTMDGANVEM EE+G DNMF+FG+   +V  L   GY P  ++E+ PELK  LEQI++ YFS +NP +F DIYNS+ + DRF+LCAD+ DYM+AQ  V   YK++  W   M+ NIA SGKFSSDRTI +YA++IWGVEP+ + LPPPF
Sbjct:   80 LNGALTIGTMDGANVEMCEEMGRDNMFVFGLTVDEVEALYKKGYRPGDYIEKNPELKLCLEQIRDGYFSPENPQLFADIYNSLVFDDRFLLCADYADYMRAQKEVEAAYKDETRWARMMLMNIASSGKFSSDRTIRDYARDIWGVEPSTVKLPPPF 235
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: G7YRK1 (Alpha-1,4 glucan phosphorylase OS=Clonorchis sinensis OX=79923 GN=CLF_108361 PE=3 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 5.568e-63
Identity = 93/156 (59.62%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            LNGALTIGTMDGANVEM EE+G +NMF+FG+   +V  L+  GY P  ++E+ PELK  LEQI++ YFS +NP +F DIYNS+ + DRF+LCAD+ DYM AQ  V   YK++  W   M+ NIA SGKFSSDRTI +YA++IWGVEP+ + LPPPF
Sbjct:   75 LNGALTIGTMDGANVEMCEEMGRENMFVFGLTVDEVDALHKKGYRPGDYIEKNPELKLCLEQIRDGYFSPENPQLFTDIYNSLVFDDRFLLCADYADYMHAQKEVEVAYKDETRWARMMLMNIASSGKFSSDRTIRDYARDIWGVEPSTVKLPPPF 230
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A3P7EFG8 (Alpha-1,4 glucan phosphorylase OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS1659 PE=3 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 8.587e-62
Identity = 90/156 (57.69%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            +NGALT+GT+DGAN+EM+EE G++NMFIFGM   +V +L+  GYD +K++  CPELK +L QI+  YFS + P +F DIY ++ + DRFMLCADF DYM+AQ  V+K Y+N+  W   M+ANIA S KFSSDRTI EYA++IWGVEP    +P PF
Sbjct:   42 VNGALTLGTLDGANIEMSEECGKENMFIFGMTVHEVEELSRKGYDSQKYINACPELKLILSQIEQGYFSPEEPDLFKDIYKTMMFGDRFMLCADFADYMRAQAEVDKAYRNEKRWAQMMLANIAGSWKFSSDRTIREYARDIWGVEPREEKMPAPF 197
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A0R3WLN4 (Alpha-1,4 glucan phosphorylase OS=Hydatigena taeniaeformis OX=6205 PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 8.872e-62
Identity = 90/156 (57.69%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPF 528
            +NGALT+GT+DGAN+EM+EE G++NMFIFGM   +V +L+  GYD +K++  CPELK +L QI+  YFS + P +F DIY ++ + DRFMLCADF DYM+AQ  V+K Y+N+  W   M+ANIA S KFSSDRTI EYA++IWGVEP    +P PF
Sbjct:   42 VNGALTLGTLDGANIEMSEECGKENMFIFGMTVHEVEELSRKGYDSQKYINACPELKLILSQIEQGYFSPEEPDLFKDIYKTMMFGDRFMLCADFADYMRAQAEVDKAYRNEKRWAQMMLANIAGSWKFSSDRTIREYARDIWGVEPREEKMPAPF 197
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A0R3TRP8 (Alpha-1,4 glucan phosphorylase OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS10268 PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.703e-61
Identity = 98/158 (62.03%), Postives = 121/158 (76.58%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFSE 534
            LNGALTIGTMDGAN+EM EE G +NMFIFGM  + V +LN  GY+ ++F+  CPELKQVLEQ+ N +FS D P +F DIY ++ Y DRFMLCADFKDYM+ Q  V K Y+++ +WG K++ANIA + KFSSDRTI EYAK+IWGVEP    +P PF E
Sbjct:  466 LNGALTIGTMDGANIEMCEECGRENMFIFGMTVEQVDELNRKGYNSQEFINACPELKQVLEQLNNGFFSPDQPDLFRDIYKTMMYGDRFMLCADFKDYMRVQAEVEKAYEDEMLWGRKVVANIAGAWKFSSDRTIREYAKDIWGVEPRVEKMPAPFEE 623
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: ENSAMXT00000050113.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002390.1:45301:47489:-1 gene:ENSAMXG00000032133.1 transcript:ENSAMXT00000050113.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 191.43 bits (485), Expect = 4.314e-62
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  ++  GY+ +++ +  PEL+QV++QI+  +FS   P +F DI N +  HDRF + AD++ Y+  Q+ VN  YKN   W  K++ NIA SGKFSSDRTISEYA+EIWGVEP+ + +PPP
Sbjct:    5 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVEAMDRKGYNAQEYYDRLPELRQVMDQIRTGFFSPAEPEMFKDIVNMLMNHDRFKVFADYEAYISCQEKVNNLYKNPKDWTKKVIRNIAASGKFSSDRTISEYAREIWGVEPSAVKIPPP 159
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygb (phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZDB-GENE-040928-2])

HSP 1 Score: 191.43 bits (485), Expect = 2.874e-56
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR +DV  ++  GY+ +++ +  PEL+QV++QI+  +FS   P +F DI N +  HDRF + AD++ Y+  Q+ VN  YKN   W  K++ NIA SGKFSSDRTISEYA+EIWGVEP+ + +PPP
Sbjct:  675 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVEAMDRKGYNAQEYYDRLPELRQVMDQIRTGFFSPAEPEMFKDIVNMLMNHDRFKVFADYEAYISCQEKVNNLYKNPKDWTKKVIRNIAASGKFSSDRTISEYAREIWGVEPSAVKIPPP 829
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygl (glycogen phosphorylase L [Source:NCBI gene;Acc:103026524])

HSP 1 Score: 182.185 bits (461), Expect = 6.252e-53
Identity = 82/155 (52.90%), Postives = 115/155 (74.19%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGT+DGANVEMAEE G++N+FIFGM   +V  ++  GYD  K+ +  PELKQ ++QIK  +FS   P +F+DI N ++ HDRF + AD++ Y+K Q+ V+  Y++   W   ++ NIA +GKFSSDRTI++YA +IWGVEPT++ +PPP
Sbjct:  682 LNGALTIGTLDGANVEMAEEAGQENLFIFGMTVDEVAAMDKEGYDAMKYYKSIPELKQAIDQIKTGFFSPKQPELFSDIINMLFNHDRFKVFADYEAYVKCQEKVSALYEHPKEWTQMVIKNIAAAGKFSSDRTITDYATQIWGVEPTDLKIPPP 836
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygma (phosphorylase, glycogen, muscle A [Source:ZFIN;Acc:ZDB-GENE-050522-482])

HSP 1 Score: 181.03 bits (458), Expect = 1.741e-52
Identity = 86/155 (55.48%), Postives = 111/155 (71.61%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE N+FIFGMR +DV  ++  GY+  ++    PELKQ ++QI   +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YK    W  K++ NIA SGKFSSDRTIS+YA+EIWGVEP+   +  P
Sbjct:  683 LNGALTIGTMDGANVEMAEEAGEGNLFIFGMRVEDVEAMDKKGYNASEYYNRIPELKQAMDQIAGGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKKPKEWTKKVIYNIAGSGKFSSDRTISQYAREIWGVEPSLEKIAAP 837
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygma (phosphorylase, glycogen, muscle A [Source:ZFIN;Acc:ZDB-GENE-050522-482])

HSP 1 Score: 181.03 bits (458), Expect = 2.391e-52
Identity = 86/155 (55.48%), Postives = 111/155 (71.61%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE N+FIFGMR +DV  ++  GY+  ++    PELKQ ++QI   +FS   P +F DI N + +HDRF + AD++DY+K Q+ V+  YK    W  K++ NIA SGKFSSDRTIS+YA+EIWGVEP+   +  P
Sbjct:  732 LNGALTIGTMDGANVEMAEEAGEGNLFIFGMRVEDVEAMDKKGYNASEYYNRIPELKQAMDQIAGGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKKPKEWTKKVIYNIAGSGKFSSDRTISQYAREIWGVEPSLEKIAAP 886
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008899.1 (pep scaffold:Pmarinus_7.0:GL479616:11553:30183:-1 gene:ENSPMAG00000007996.1 transcript:ENSPMAT00000008899.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 197.593 bits (501), Expect = 1.852e-59
Identity = 89/155 (57.42%), Postives = 119/155 (76.77%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  +V  ++  GY  + + E  PEL+Q ++QI + +FS  +P VF D+ N + +HDRF + ADF+DY+K Q+ V++ YKN   W +K++ NIA SGKFSSDRTI+EYA+EIWGVEP+ + +PPP
Sbjct:  603 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVDEVEAMDKKGYSARDYYERLPELQQAIDQISSGFFSPKHPHVFEDVVNMLMHHDRFKVFADFEDYVKCQEKVSELYKNPREWTIKVIRNIAASGKFSSDRTIAEYAREIWGVEPSRVKIPPP 757
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006065.1 (pep scaffold:Pmarinus_7.0:GL476342:314148:364539:-1 gene:ENSPMAG00000005334.1 transcript:ENSPMAT00000006065.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 192.2 bits (487), Expect = 3.363e-57
Identity = 90/155 (58.06%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV  ++ TGYD + + ++ PEL+QV++Q+ + +FS   P +F DI N + +HDRF + AD++ YM AQD V+  Y+N   W  K++ NIA S KFSSDRTISEYA+EIWGVEPT   +P P
Sbjct:  685 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVPDVEAMDKTGYDAQYYYDKLPELRQVIDQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYESYMVAQDKVSALYQNPKEWTKKVIHNIAGSAKFSSDRTISEYAREIWGVEPTYKKIPAP 839
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Yeast
Match: GPH1 (Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; phosphorylation by Cdc28p may coordinately regulate carbohydrate metabolism and the cell cycle; expression is regulated by stress-response elements and by the HOG MAP kinase pathway [Source:SGD;Acc:S000006364])

HSP 1 Score: 112.464 bits (280), Expect = 2.535e-29
Identity = 62/152 (40.79%), Postives = 93/152 (61.18%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECP-ELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYH-DRFMLCADFKDYMKAQDLVNKCYKNQYV-WGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTN 507
            +NG L IGT+DGANVE+  E+GEDN+F+FG   ++V +L    Y+ +   ++ P  L  VL  I++  FS +NP  F  + +SI YH D +++  DF+ Y+   +LV++ + NQ   W  K + ++A  G FSSDR I EY+  IW VEP  
Sbjct:  754 MNGGLIIGTVDGANVEITREIGEDNVFLFGNLSENVEELR---YNHQYHPQDLPSSLDSVLSYIESGQFSPENPNEFKPLVDSIKYHGDYYLVSDDFESYLATHELVDQEFHNQRSEWLKKSVLSVANVGFFSSDRCIEEYSDTIWNVEPVT 902
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Match: EDO33811 (Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7SR65])

HSP 1 Score: 177.178 bits (448), Expect = 1.331e-51
Identity = 81/145 (55.86%), Postives = 108/145 (74.48%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGV 495
            +NGALTIGT+DGANVEM EE+G++N+FIFGM  ++V  L+  GY+PK + E  PELK+ L+QI++NYFS   PGVF D+ NS+ YHDRF L ADF  Y+K Q+ V++ +     W    + N+A SGKFS+DRTI +YA+EIWGV
Sbjct:  635 MNGALTIGTLDGANVEMMEEMGKENIFIFGMTVEEVEALSRKGYNPKTYYERNPELKKALDQIRDNYFSPKEPGVFQDVVNSLLYHDRFYLLADFDAYLKCQERVSQTFMKPEKWCTMALMNVASSGKFSTDRTIRQYAEEIWGV 779
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Match: EDO27771 (Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7T8B9])

HSP 1 Score: 96.6709 bits (239), Expect = 1.773e-25
Identity = 59/154 (38.31%), Postives = 90/154 (58.44%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPP 522
            +NGALTIGT+DGANVEM EE+G++N+FIFGM  ++V  L+  G DP         LK+++  + N   + D        +  + +  RF L ADF  Y+K Q+ V++    ++      MA +    +FS+DRTI +YA+EIWGV+  +  + P
Sbjct:   28 MNGALTIGTLDGANVEMMEEMGKENIFIFGMTVEEVEALSRKG-DP---------LKKIIVFLANQRQNADQFSSLIFCFVVVVFC-RFYLLADFDAYLKCQERVSQTKPEKWC----TMALMWLLWEFSTDRTIRQYAEEIWGVKSVDSEVKP 166
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygl (glycogen phosphorylase L [Source:NCBI gene;Acc:101158492])

HSP 1 Score: 193.356 bits (490), Expect = 6.772e-57
Identity = 87/155 (56.13%), Postives = 118/155 (76.13%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR ++V +++  GYD   +  + PELK V++QI + +FS  NP +F D+   ++ HDRF + ADF+DYMK Q+ V++ Y+N   W   ++ NIA +GKFSSDRTI+EYA E+WGVEPT++ +PPP
Sbjct:  682 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEEVAEMDKKGYDAMAYYNKIPELKHVMDQITSGFFSPKNPDLFKDLTEMLFKHDRFKVFADFEDYMKCQEKVSQLYQNPVEWTKMVIRNIAGTGKFSSDRTITEYATEVWGVEPTDLKIPPP 836
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygmb (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:101171113])

HSP 1 Score: 187.578 bits (475), Expect = 8.666e-55
Identity = 87/155 (56.13%), Postives = 112/155 (72.26%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GE+N+FIFGMR  DV  L+  GYD   +    PELKQ ++QI   +FS D+PG+F D+ N + +HDRF + AD++DY++ QD V+  YK+   W  K++ NIA  GKFSSDRTIS+YA+EIWG+EP    +  P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEENLFIFGMRVDDVEALDKKGYDAMSYYNRIPELKQAVDQISGGFFSPDHPGLFKDLVNMLMHHDRFKVFADYEDYIQCQDKVSALYKDTKAWTKKVIHNIAGCGKFSSDRTISQYAREIWGMEPNLEKIAAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygma (glycogen phosphorylase, muscle form [Source:NCBI gene;Acc:101169133])

HSP 1 Score: 185.652 bits (470), Expect = 3.226e-54
Identity = 89/155 (57.42%), Postives = 112/155 (72.26%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGANVEMAEE GEDN+FIFGMR  DV  L+  GY+ +++    PELKQ ++QI   YFS   P +F +I N + +HDRF + AD++DY+K Q+ VN  YKN   W  K++ NIA  GKFSSDRTI++YA+EIWG+EPT   L  P
Sbjct:  684 LNGALTIGTMDGANVEMAEEAGEDNLFIFGMRVDDVDALDRKGYNAEEYYSRLPELKQAIDQIAGGYFSPKQPDLFKEIVNMLMHHDRFKVFADYEDYIKCQEKVNALYKNPKEWTKKVIYNIAGCGKFSSDRTIAQYAREIWGMEPTLERLAAP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygb (glycogen phosphorylase B [Source:NCBI gene;Acc:101170949])

HSP 1 Score: 176.407 bits (446), Expect = 7.471e-51
Identity = 81/155 (52.26%), Postives = 114/155 (73.55%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            LNGALTIGTMDGA VEMAEE GE+N+FIFG++  +V +L+  GY+ K++ +  PELK  ++QI+  YFS   P +F ++   +  HDRF + AD++ Y+K Q+ V++ Y+N   W   ++ NIA +GKFSSDRTIS+YA+EIWGVEP+N+ LP P
Sbjct:  684 LNGALTIGTMDGATVEMAEEAGEENLFIFGLKVAEVDELDRKGYNAKEYYDSLPELKLAVDQIQRGYFSPSEPELFREVTEMLMKHDRFKVFADYEAYVKRQEQVSELYENPREWTKVVIRNIAAAGKFSSDRTISQYAREIWGVEPSNIKLPSP 838
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygmb (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:101171113])

HSP 1 Score: 79.337 bits (194), Expect = 2.610e-17
Identity = 39/83 (46.99%), Postives = 55/83 (66.27%), Query Frame = 1 
Query:  277 PGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPP 525
            PG  N+I N++     F + AD++DY++ QD V+  YK+   W  K++ NIA  GKFSSDRTIS+YA+EIWG+EP    +  P
Sbjct:  252 PGYRNNIVNTMRL---FKVFADYEDYIQCQDKVSALYKDTKAWTKKVIHNIAGCGKFSSDRTISQYAREIWGMEPNLEKIAAP 331
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 261.151 bits (666), Expect = 2.472e-82
Identity = 129/159 (81.13%), Postives = 131/159 (82.39%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFSED 537
            LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQ+  +                          FMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFSED
Sbjct:  679 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQITPE--------------------------FMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFSED 811
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000061417.1 (SMESG000061417.1)

HSP 1 Score: 208.764 bits (530), Expect = 1.010e-62
Identity = 96/157 (61.15%), Postives = 124/157 (78.98%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFS 531
            LNGALTIGTMDGANVEM EE+G+DNMFIFGM  ++V  L + GY+P++F+ +  ELK  L+QI++ YFS ++P +F+DIYNS+ Y DRFMLCADF DY++ QD V + YK++++W  K++ NI  SGKFSSDRTISEYA+EIW VEPT   LP P S
Sbjct:  680 LNGALTIGTMDGANVEMCEEMGKDNMFIFGMSVEEVNALGARGYNPQEFIHKNEELKLCLDQIRDGYFSPEDPRLFHDIYNSLAYQDRFMLCADFADYVRVQDHVGEVYKDEFLWATKVLHNIGSSGKFSSDRTISEYAREIWNVEPTLEKLPDPHS 836
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000042275.1 (SMESG000042275.1)

HSP 1 Score: 183.341 bits (464), Expect = 1.534e-53
Identity = 89/146 (60.96%), Postives = 110/146 (75.34%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCYKNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVE 498
            LNGALTIGTMDGANVEM EE+G++NMFIFGM   +V  L    Y+PK+F+E   EL   L QI++ +FS + P +F+DIYNS+ YHDRFMLCADF+DY+K QD V K Y+N+  W    + NIA SGKFSSDRTISEYA++IW  E
Sbjct:  667 LNGALTIGTMDGANVEMCEEMGKENMFIFGMLVDEVNSLKPR-YNPKEFIERNEELNLCLNQIRDGHFSPNQPHLFHDIYNSLVYHDRFMLCADFEDYVKVQDEVGKMYQNKRQWAKMALLNIASSGKFSSDRTISEYARDIWNSE 811
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 158.688 bits (400), Expect = 8.774e-45
Identity = 77/87 (88.51%), Postives = 79/87 (90.80%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGV-----FNDIYNS 306
            LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPG+     F  +Y S
Sbjct:  645 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGIHALCRFQGLYES 731
[Close]
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 158.688 bits (400), Expect = 8.978e-45
Identity = 77/87 (88.51%), Postives = 79/87 (90.80%), Query Frame = 1 
Query:   61 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGV-----FNDIYNS 306
            LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPG+     F  +Y S
Sbjct:  679 LNGALTIGTMDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQVLEQIKNNYFSQDNPGIHALCRFQGLYES 765
The following BLAST results are available for this feature:
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PYGM1.089e-5757.42glycogen phosphorylase, muscle associated [Source:... [more]
PYGM4.700e-5757.42glycogen phosphorylase, muscle associated [Source:... [more]
PYGB3.086e-5555.48glycogen phosphorylase B [Source:HGNC Symbol;Acc:H... [more]
PYGL2.651e-5457.24glycogen phosphorylase L [Source:HGNC Symbol;Acc:H... [more]
PYGL3.598e-5457.24glycogen phosphorylase L [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pygl-11.811e-5750.64Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-12.974e-5350.64Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-12.800e-5250.64Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-13.375e-5250.64Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GlyP4.420e-5056.13gene:FBgn0004507 transcript:FBtr0100485[more]
GlyP4.420e-5056.13gene:FBgn0004507 transcript:FBtr0077828[more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pygl3.422e-5858.71phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZD... [more]
pygl3.556e-5858.71phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZD... [more]
pygb6.732e-5757.42phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZD... [more]
pygb7.233e-5757.42phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZD... [more]
pygb7.750e-5757.42phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZD... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PYGM1.049e-5858.06glycogen phosphorylase, muscle associated [Source:... [more]
PYGM1.511e-5858.06glycogen phosphorylase, muscle associated [Source:... [more]
PYGM1.932e-5858.06glycogen phosphorylase, muscle associated [Source:... [more]
PYGM1.942e-5858.06glycogen phosphorylase, muscle associated [Source:... [more]
PYGM1.971e-5858.06glycogen phosphorylase, muscle associated [Source:... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pygb7.324e-5856.77brain glycogen phosphorylase [Source:MGI Symbol;Ac... [more]
Pygm2.574e-5756.13muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm2.574e-5756.13muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm7.664e-5756.13muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm7.664e-5756.13muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8CI94|PYGB_MOUSE5.123e-5756.77Glycogen phosphorylase, brain form OS=Mus musculus... [more]
sp|Q8HXW4|PYGM_MACFA1.050e-5657.42Glycogen phosphorylase, muscle form OS=Macaca fasc... [more]
sp|P11217|PYGM_HUMAN2.257e-5657.42Glycogen phosphorylase, muscle form OS=Homo sapien... [more]
sp|P09812|PYGM_RAT2.705e-5656.13Glycogen phosphorylase, muscle form OS=Rattus norv... [more]
sp|P53534|PYGB_RAT4.714e-5656.77Glycogen phosphorylase, brain form (Fragment) OS=R... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S8WR114.766e-6360.26Alpha-1,4 glucan phosphorylase (Fragment) OS=Opist... [more]
G7YRK15.568e-6359.62Alpha-1,4 glucan phosphorylase OS=Clonorchis sinen... [more]
A0A3P7EFG88.587e-6257.69Alpha-1,4 glucan phosphorylase OS=Hydatigena taeni... [more]
A0A0R3WLN48.872e-6257.69Alpha-1,4 glucan phosphorylase OS=Hydatigena taeni... [more]
A0A0R3TRP81.703e-6162.03Alpha-1,4 glucan phosphorylase OS=Rodentolepis nan... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000050113.14.314e-6257.42pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
pygb2.874e-5657.42phosphorylase, glycogen; brain [Source:ZFIN;Acc:ZD... [more]
pygl6.252e-5352.90glycogen phosphorylase L [Source:NCBI gene;Acc:103... [more]
pygma1.741e-5255.48phosphorylase, glycogen, muscle A [Source:ZFIN;Acc... [more]
pygma2.391e-5255.48phosphorylase, glycogen, muscle A [Source:ZFIN;Acc... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000008899.11.852e-5957.42pep scaffold:Pmarinus_7.0:GL479616:11553:30183:-1 ... [more]
ENSPMAT00000006065.13.363e-5758.06pep scaffold:Pmarinus_7.0:GL476342:314148:364539:-... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GPH12.535e-2940.79Glycogen phosphorylase required for the mobilizati... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO338111.331e-5155.86Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7SR65... [more]
EDO277711.773e-2538.31Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7T8B9... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pygl6.772e-5756.13glycogen phosphorylase L [Source:NCBI gene;Acc:101... [more]
pygmb8.666e-5556.13glycogen phosphorylase, muscle associated [Source:... [more]
pygma3.226e-5457.42glycogen phosphorylase, muscle form [Source:NCBI g... [more]
pygb7.471e-5152.26glycogen phosphorylase B [Source:NCBI gene;Acc:101... [more]
pygmb2.610e-1746.99glycogen phosphorylase, muscle associated [Source:... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000013460.12.472e-8281.13SMESG000013460.1[more]
SMESG000061417.11.010e-6261.15SMESG000061417.1[more]
SMESG000042275.11.534e-5360.96SMESG000042275.1[more]
SMESG000013460.18.774e-4588.51SMESG000013460.1[more]
SMESG000013460.18.978e-4588.51SMESG000013460.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006686 ID=SMED30006686|Name=Alpha-1,4 glucan phosphorylase|organism=Schmidtea mediterranea sexual|type=transcript|length=627bp
AATAATAATAATAATAATAATTTTCATTATCAAAATAGTATTTGATTTAA
TAATTATTAGTTGAACGGAGCTTTAACAATAGGAACTATGGATGGAGCCA
ATGTTGAAATGGCCGAGGAAGTTGGTGAGGATAATATGTTCATTTTTGGA
ATGAGAGAAAAAGACGTGATAAAATTAAATTCTACCGGATATGATCCAAA
GAAGTTTGTCGAGGAATGTCCCGAATTAAAACAAGTTCTTGAACAAATAA
AAAACAACTATTTCTCACAAGATAACCCCGGAGTATTCAACGATATTTAT
AATTCAATTTATTATCATGACAGATTCATGCTCTGTGCCGATTTCAAGGA
TTATATGAAAGCTCAAGATCTCGTCAATAAATGCTATAAAAATCAGTACG
TGTGGGGTTTGAAGATGATGGCGAACATAGCAAAATCAGGAAAGTTTTCA
TCTGACAGAACAATATCAGAATATGCCAAAGAAATATGGGGCGTCGAGCC
AACAAATATGCCGCTTCCACCGCCATTTAGTGAAGACTAGTGAAATAGAG
TGATATGACTTTATATTTATTGAGATATTGATATTAGTTATTTATAGTTT
TTATTTGTTTAAAACATTGTGATTATG
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protein sequence of SMED30006686-orf-1

>SMED30006686-orf-1 ID=SMED30006686-orf-1|Name=SMED30006686-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp
MDGANVEMAEEVGEDNMFIFGMREKDVIKLNSTGYDPKKFVEECPELKQV
LEQIKNNYFSQDNPGVFNDIYNSIYYHDRFMLCADFKDYMKAQDLVNKCY
KNQYVWGLKMMANIAKSGKFSSDRTISEYAKEIWGVEPTNMPLPPPFSED
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
GO:0008184glycogen phosphorylase activity
GO:0004645phosphorylase activity
GO:0030170pyridoxal phosphate binding
Vocabulary: INTERPRO
TermDefinition
IPR000811Glyco_trans_35
Vocabulary: biological process
TermDefinition
GO:0005975carbohydrate metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.2000coord: 1..121
e-value: 7.0E-44
score: 152.3
NoneNo IPR availablePANTHERPTHR11468:SF13GLYCOGEN PHOSPHORYLASE, MUSCLE FORMcoord: 1..146
NoneNo IPR availableCDDcd04300GT35_Glycogen_Phosphorylasecoord: 1..136
e-value: 5.6885E-65
score: 209.305
NoneNo IPR availableSUPERFAMILYSSF53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 1..142
IPR000811Glycosyl transferase, family 35PFAMPF00343Phosphorylasecoord: 1..136
e-value: 2.4E-49
score: 168.3
IPR000811Glycosyl transferase, family 35PANTHERPTHR11468GLYCOGEN PHOSPHORYLASEcoord: 1..146