Pleckstrin homology like domain family B member 2

Overview
NamePleckstrin homology like domain family B member 2
Smed IDSMED30006683
Length (bp)1472
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pleckstrin homology like domain family B member 2 (SMED30006683) t-SNE clustered cells

Violin plots show distribution of expression levels for Pleckstrin homology like domain family B member 2 (SMED30006683) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pleckstrin homology like domain family B member 2 (SMED30006683) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pleckstrin homology like domain family B member 2 (SMED30006683) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006683

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30006683SMESG000033071.1 dd_Smed_v4_7833_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30006683SMESG000033071.1 dd_Smed_v4_7833_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30006683SMESG000033071.1 dd_Smed_v4_7833_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006683SMESG000033071.1 dd_Smed_v4_7833_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30006683SMESG000033071.1 dd_Smed_v4_7833_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Match: PHLDB2 (pleckstrin homology like domain family B member 2 [Source:HGNC Symbol;Acc:HGNC:29573])

HSP 1 Score: 107.457 bits (267), Expect = 1.034e-24
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct:  271 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCYH--VSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 413
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Match: PHLDB2 (pleckstrin homology like domain family B member 2 [Source:HGNC Symbol;Acc:HGNC:29573])

HSP 1 Score: 108.227 bits (269), Expect = 2.890e-24
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct:  597 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCY--HVSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 739
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Match: PHLDB2 (pleckstrin homology like domain family B member 2 [Source:HGNC Symbol;Acc:HGNC:29573])

HSP 1 Score: 107.457 bits (267), Expect = 6.288e-24
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1106 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCY--HVSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1248
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Match: PHLDB2 (pleckstrin homology like domain family B member 2 [Source:HGNC Symbol;Acc:HGNC:29573])

HSP 1 Score: 107.457 bits (267), Expect = 6.288e-24
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1106 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCY--HVSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1248
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Match: PHLDB2 (pleckstrin homology like domain family B member 2 [Source:HGNC Symbol;Acc:HGNC:29573])

HSP 1 Score: 107.457 bits (267), Expect = 6.845e-24
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1090 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCY--HVSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1232
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Match: CG5004 (gene:FBgn0260748 transcript:FBtr0346797)

HSP 1 Score: 78.9518 bits (193), Expect = 1.972e-16
Identity = 51/158 (32.28%), Postives = 73/158 (46.20%), Query Frame = 2 
Query:  869 RPLTRYLPNPNDPRFDLRNYIEMCGHNLS-CVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEA 1339
            RPLTRYLP    P  +LR++IE  GH +  C   F  +D+HSC G L KL G     W ++ ++ DR R    YY+  +  K +G   F  I  ++      SK G       H  F +         +  K    L A +  + R+W+D I TGA+ 
Sbjct:   78 RPLTRYLPI-FSPDLNLRHHIETAGHQIDPCPHVF--VDAHSCRGYLHKL-GATFHAWSRRWFVLDRQRSALIYYSDKSERKPRGGAYFATIDEVYLDHLNASKSGR-----PHCTFIV---------KTKKRSYNLQAASDSAARIWIDAIITGAQG 217
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Match: CG5004 (gene:FBgn0260748 transcript:FBtr0343201)

HSP 1 Score: 79.337 bits (194), Expect = 3.050e-16
Identity = 51/158 (32.28%), Postives = 73/158 (46.20%), Query Frame = 2 
Query:  869 RPLTRYLPNPNDPRFDLRNYIEMCGHNLS-CVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEA 1339
            RPLTRYLP    P  +LR++IE  GH +  C   F  +D+HSC G L KL G     W ++ ++ DR R    YY+  +  K +G   F  I  ++      SK G       H  F +         +  K    L A +  + R+W+D I TGA+ 
Sbjct:  134 RPLTRYLPI-FSPDLNLRHHIETAGHQIDPCPHVF--VDAHSCRGYLHKL-GATFHAWSRRWFVLDRQRSALIYYSDKSERKPRGGAYFATIDEVYLDHLNASKSGR-----PHCTFIV---------KTKKRSYNLQAASDSAARIWIDAIITGAQG 273
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Match: CG5004 (gene:FBgn0260748 transcript:FBtr0302377)

HSP 1 Score: 80.1073 bits (196), Expect = 3.319e-16
Identity = 51/160 (31.87%), Postives = 74/160 (46.25%), Query Frame = 2 
Query:  869 RPLTRYLPNPNDPRFDLRNYIEMCGHNLS-CVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYM 1345
            RPLTRYLP    P  +LR++IE  GH +  C   F  +D+HSC G L KL G     W ++ ++ DR R    YY+  +  K +G   F  I  ++      SK G       H  F +         +  K    L A +  + R+W+D I TGA+  +
Sbjct:  185 RPLTRYLPI-FSPDLNLRHHIETAGHQIDPCPHVF--VDAHSCRGYLHKL-GATFHAWSRRWFVLDRQRSALIYYSDKSERKPRGGAYFATIDEVYLDHLNASKSGR-----PHCTFIV---------KTKKRSYNLQAASDSAARIWIDAIITGAQGNL 326
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Match: CG5004 (gene:FBgn0260748 transcript:FBtr0302375)

HSP 1 Score: 81.2629 bits (199), Expect = 7.291e-16
Identity = 51/160 (31.87%), Postives = 74/160 (46.25%), Query Frame = 2 
Query:  869 RPLTRYLPNPNDPRFDLRNYIEMCGHNLS-CVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYM 1345
            RPLTRYLP    P  +LR++IE  GH +  C   F  +D+HSC G L KL G     W ++ ++ DR R    YY+  +  K +G   F  I  ++      SK G       H  F +         +  K    L A +  + R+W+D I TGA+  +
Sbjct: 1004 RPLTRYLPI-FSPDLNLRHHIETAGHQIDPCPHVF--VDAHSCRGYLHKL-GATFHAWSRRWFVLDRQRSALIYYSDKSERKPRGGAYFATIDEVYLDHLNASKSGRP-----HCTFIV---------KTKKRSYNLQAASDSAARIWIDAIITGAQGNL 1145
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Match: CG5004 (gene:FBgn0260748 transcript:FBtr0302376)

HSP 1 Score: 81.2629 bits (199), Expect = 7.291e-16
Identity = 51/160 (31.87%), Postives = 74/160 (46.25%), Query Frame = 2 
Query:  869 RPLTRYLPNPNDPRFDLRNYIEMCGHNLS-CVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYM 1345
            RPLTRYLP    P  +LR++IE  GH +  C   F  +D+HSC G L KL G     W ++ ++ DR R    YY+  +  K +G   F  I  ++      SK G       H  F +         +  K    L A +  + R+W+D I TGA+  +
Sbjct: 1004 RPLTRYLPI-FSPDLNLRHHIETAGHQIDPCPHVF--VDAHSCRGYLHKL-GATFHAWSRRWFVLDRQRSALIYYSDKSERKPRGGAYFATIDEVYLDHLNASKSGRP-----HCTFIV---------KTKKRSYNLQAASDSAARIWIDAIITGAQGNL 1145
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Match: phldb2a (pleckstrin homology-like domain, family B, member 2a [Source:ZFIN;Acc:ZDB-GENE-091118-9])

HSP 1 Score: 110.153 bits (274), Expect = 5.701e-25
Identity = 67/161 (41.61%), Postives = 89/161 (55.28%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHN-LSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            SRPLTRYLP   D  FDLR +IE  GH+  +C    L I   +C G L K+ GK +KTWKK+ ++FDRNRR  AYY     TK+KG+I F  I  ++          +   S N ++ FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1050 SRPLTRYLPVRKD-EFDLRGHIEAAGHHPETCF--HLAITEKTCRGFLVKMGGK-IKTWKKRWFVFDRNRRTLAYYADKHETKMKGVIYFQAIEEVYY-----DHLKNAHKSPNPSLTFSVKTHDRV---------YYMVAPSPEAMRIWMDVIVTGAEGY 1192
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Match: phldb2b (pleckstrin homology-like domain, family B, member 2b [Source:ZFIN;Acc:ZDB-GENE-030131-3235])

HSP 1 Score: 107.457 bits (267), Expect = 4.462e-24
Identity = 65/161 (40.37%), Postives = 88/161 (54.66%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            SRPLTRYLP   D  FDLR +I+  GHN  +C    + I   +C G L K+ GK +KTWKK+ ++FDRNRR  +YY      K+KG+I F  I  ++          S   S N ++ FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1171 SRPLTRYLPVRKD-DFDLRAHIDSAGHNTETCY--HISITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNRRTLSYYADKHEAKLKGVIYFQAIEEVYY-----DHLKSAHKSPNPSLTFSVKTHDRV---------YYMVAPSPEAMRIWMDVIVTGAEGY 1313
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 100.138 bits (248), Expect = 8.748e-24
Identity = 61/168 (36.31%), Postives = 91/168 (54.17%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR++IE  GH++ +C    L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     +K+KG+I F  I  +   H  S T S   ++        F ++  D             + AP++E+ R+WMDVI TGAE Y   +N
Sbjct:   64 ARPMTRYLPIRKEE-FDLRSHIESSGHSVDTCYHVIL--TEKMCKGFLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHESKLKGVIYFQAIEEVYYDHLRSATKSPTPTL-------TFCVKTHDRL---------YYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 211
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 104.76 bits (260), Expect = 3.297e-23
Identity = 61/170 (35.88%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR++IE  GH++ +C    L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     +K+KG+I F  I  ++        +  + S+     F++ L ++      F     D L  + AP++E+ R+WMDVI TGAE Y   +N
Sbjct: 1286 ARPMTRYLPIRKE-EFDLRSHIESSGHSVDTCYHVILT--EKMCKGFLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHESKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPTPTLTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 1443
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Match: phldb1a (pleckstrin homology-like domain, family B, member 1a [Source:ZFIN;Acc:ZDB-GENE-060526-272])

HSP 1 Score: 102.834 bits (255), Expect = 1.357e-22
Identity = 60/163 (36.81%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLP   +  FDLR+++E  GH++ +C   ++ +    C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1143 ARPMTRYLPIRKE-EFDLRSHVESSGHSVDTCA--YVIVTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRTFSYYVDKHETKLKGVIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YFMVAPSPEAMRIWMDVIVTGAEGY 1285
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Match: mdm1 (Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-991706])

HSP 1 Score: 103.99 bits (258), Expect = 7.940e-23
Identity = 59/169 (34.91%), Postives = 90/169 (53.25%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLP   +  FDLR ++E  GH +      + +    C G L K+ GK +K+WKK+ ++FDR +R  +YY      K+KG+I F  I  ++        +  + S+    +F++ L  + N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1305 ARPLTRYLPIRKE-DFDLRAHVESSGHAVDACPHII-LSEKMCRGFLTKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVY--------YDHLRSAAKKGLFNLSLATSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1462
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Match: mdm1 (Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-991706])

HSP 1 Score: 95.5153 bits (236), Expect = 4.154e-20
Identity = 57/165 (34.55%), Postives = 84/165 (50.91%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLP   +  FDLR ++E  GH +      + +    C G L K+ GK +K+WKK+ ++FDR +R  +YY      K+KG+I F  I  ++          S   S N  + F ++  D             + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1348 ARPLTRYLPIRKE-DFDLRAHVESSGHAVDACPHII-LSEKMCRGFLTKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1495
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Match: mdm1 (Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-991706])

HSP 1 Score: 95.5153 bits (236), Expect = 4.470e-20
Identity = 57/165 (34.55%), Postives = 84/165 (50.91%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLP   +  FDLR ++E  GH +      + +    C G L K+ GK +K+WKK+ ++FDR +R  +YY      K+KG+I F  I  ++          S   S N  + F ++  D             + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1271 ARPLTRYLPIRKE-DFDLRAHVESSGHAVDACPHII-LSEKMCRGFLTKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1418
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Match: PHLDB3 (pleckstrin homology-like domain, family B, member 3 [Source:NCBI gene;Acc:100491291])

HSP 1 Score: 86.6557 bits (213), Expect = 2.176e-17
Identity = 51/144 (35.42%), Postives = 76/144 (52.78%), Query Frame = 2 
Query:  911 FDLRNYIEMCGHNLSCVET--FLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAE 1336
             DLR+++E  GH   CVET   +++    C G L K+ G+ +KTW+K+ ++FDR +R  AYYT     K+KG+I F  I  ++      S F S      H   +  L       + F+    + APT E+ R+WMDV+ T AE
Sbjct:  502 LDLRSHLEASGH---CVETCPHVRVSPTCCKGYLVKMGGR-IKTWRKRWFVFDRQKRRLAYYTDKDEVKLKGVIYFQAIEEVY-YDHLRSAFKS-----PHPKLTFCL-------KTFERLFCMVAPTPEAMRIWMDVMVTAAE 628
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Match: PHLDB3 (pleckstrin homology-like domain, family B, member 3 [Source:NCBI gene;Acc:100491291])

HSP 1 Score: 86.6557 bits (213), Expect = 2.176e-17
Identity = 51/144 (35.42%), Postives = 76/144 (52.78%), Query Frame = 2 
Query:  911 FDLRNYIEMCGHNLSCVET--FLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAE 1336
             DLR+++E  GH   CVET   +++    C G L K+ G+ +KTW+K+ ++FDR +R  AYYT     K+KG+I F  I  ++      S F S      H   +  L       + F+    + APT E+ R+WMDV+ T AE
Sbjct:  502 LDLRSHLEASGH---CVETCPHVRVSPTCCKGYLVKMGGR-IKTWRKRWFVFDRQKRRLAYYTDKDEVKLKGVIYFQAIEEVY-YDHLRSAFKS-----PHPKLTFCL-------KTFERLFCMVAPTPEAMRIWMDVMVTAAE 628
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Match: Phldb2 (pleckstrin homology like domain, family B, member 2 [Source:MGI Symbol;Acc:MGI:2444981])

HSP 1 Score: 108.227 bits (269), Expect = 1.474e-24
Identity = 64/182 (35.16%), Postives = 98/182 (53.85%), Query Frame = 2 
Query:  815 KQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            ++   +R K   Q    +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY      K+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct:  405 EKEVKIREKQRAQ----ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCF--HVSITEKTCRGYLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 560
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Match: Phldb1 (pleckstrin homology like domain, family B, member 1 [Source:MGI Symbol;Acc:MGI:2143230])

HSP 1 Score: 106.686 bits (265), Expect = 6.046e-24
Identity = 62/166 (37.35%), Postives = 90/166 (54.22%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLPN  +  FDL+ +IE  GH + +C+   L   S  C G L K+ GK +K+WKK+ ++FDR +R  +YY     TK+KG+I F  I  ++          S   S N  + F ++  D             + AP++E+ R+WMDVI TGAE Y   +N
Sbjct:  537 ARPLTRYLPNRKED-FDLKTHIESSGHGVDTCLHVVL--SSKVCRGYLIKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 684
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Match: Phldb1 (pleckstrin homology like domain, family B, member 1 [Source:MGI Symbol;Acc:MGI:2143230])

HSP 1 Score: 106.301 bits (264), Expect = 6.664e-24
Identity = 62/166 (37.35%), Postives = 90/166 (54.22%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLPN  +  FDL+ +IE  GH + +C+   L   S  C G L K+ GK +K+WKK+ ++FDR +R  +YY     TK+KG+I F  I  ++          S   S N  + F ++  D             + AP++E+ R+WMDVI TGAE Y   +N
Sbjct:  494 ARPLTRYLPNRKED-FDLKTHIESSGHGVDTCLHVVL--SSKVCRGYLIKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 641
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Match: Phldb2 (pleckstrin homology like domain, family B, member 2 [Source:MGI Symbol;Acc:MGI:2444981])

HSP 1 Score: 106.686 bits (265), Expect = 9.692e-24
Identity = 61/165 (36.97%), Postives = 92/165 (55.76%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY      K+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1102 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCF--HVSITEKTCRGYLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1244
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Match: Phldb2 (pleckstrin homology like domain, family B, member 2 [Source:MGI Symbol;Acc:MGI:2444981])

HSP 1 Score: 106.301 bits (264), Expect = 1.021e-23
Identity = 61/165 (36.97%), Postives = 92/165 (55.76%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY      K+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1155 ARPLTRYLPVRKE-DFDLRSHVETAGHNIDTCF--HVSITEKTCRGYLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1297
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Match: sp|Q86SQ0|PHLB2_HUMAN (Pleckstrin homology-like domain family B member 2 OS=Homo sapiens OX=9606 GN=PHLDB2 PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 3.020e-23
Identity = 62/165 (37.58%), Postives = 93/165 (56.36%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY     TK+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1106 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCY--HVSITEKTCRGFLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1248
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Match: sp|Q8K1N2|PHLB2_MOUSE (Pleckstrin homology-like domain family B member 2 OS=Mus musculus OX=10090 GN=Phldb2 PE=1 SV=2)

HSP 1 Score: 106.686 bits (265), Expect = 6.779e-23
Identity = 61/165 (36.97%), Postives = 92/165 (55.76%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIH-----AVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RPLTRYLP   +  FDLR+++E  GHN+ +C    + I   +C G L K+ GK +KTWKK+ ++FDRN+R F+YY      K+KG+I F  I  ++       +++P+   +         FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1102 ARPLTRYLPVRKED-FDLRSHVETAGHNIDTCF--HVSITEKTCRGYLIKMGGK-IKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLT---------FSVKTHDRI---------YYMVAPSPEAMRIWMDVIVTGAEGY 1244
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Match: sp|Q63312|PHLB1_RAT (Pleckstrin homology-like domain family B member 1 (Fragment) OS=Rattus norvegicus OX=10116 GN=Phldb1 PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 9.760e-23
Identity = 62/166 (37.35%), Postives = 90/166 (54.22%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLPN  +  FDL+ +IE  GH + +C+   L   S  C G L K+ GK +K+WKK+ ++FDR +R  +YY     TK+KG+I F  I  ++          S   S N  + F ++  D             + AP++E+ R+WMDVI TGAE Y   +N
Sbjct:  684 ARPLTRYLPNRKED-FDLKTHIESSGHGVDTCLHVVL--SSKVCRGYLIKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 831
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Match: sp|Q6PDH0|PHLB1_MOUSE (Pleckstrin homology-like domain family B member 1 OS=Mus musculus OX=10090 GN=Phldb1 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.019e-20
Identity = 62/166 (37.35%), Postives = 90/166 (54.22%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLPN  +  FDL+ +IE  GH + +C+   L   S  C G L K+ GK +K+WKK+ ++FDR +R  +YY     TK+KG+I F  I  ++          S   S N  + F ++  D             + AP++E+ R+WMDVI TGAE Y   +N
Sbjct: 1224 ARPLTRYLPNRKED-FDLKTHIESSGHGVDTCLHVVLS--SKVCRGYLIKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYY-----DHLRSAAKSPNPALTFCVKTHDRL---------YYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 1371
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Match: sp|Q86UU1|PHLB1_HUMAN (Pleckstrin homology-like domain family B member 1 OS=Homo sapiens OX=9606 GN=PHLDB1 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 9.982e-20
Identity = 62/171 (36.26%), Postives = 94/171 (54.97%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLI-DNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RPLTRYLP   +  FDL+ +IE  GH + +C+   L   S  C G L K+ GK +K+WKK+ ++FDR +R  +YY     TK+KG+I F  I  ++        +  + S+     F   ++ ++ N    F     D L  + AP++E+ R+WMDVI TGAE Y   +N
Sbjct: 1219 ARPLTRYLPIRKED-FDLKTHIESSGHGVDTCLHVVLS--SKVCRGYLVKMGGK-IKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVY--------YDHLRSAAKKRFFRFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN 1377
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Match: A0A1E1X9M3 (Putative pleckstrin similarity domain protein (Fragment) OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.860e-24
Identity = 61/168 (36.31%), Postives = 90/168 (53.57%), Query Frame = 2 
Query:  851 QTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTL 1354
            + +   RPLTRYLP       DLR ++E  GH +      ++I S SC G L KL GK  ++WKK+ ++FDRNRR   Y++  T TK+KG + F  I  ++          S+ S +    F +         + ++    L APT+E+ R+W+DVIFTGAE Y+  L
Sbjct:   35 EKQKAQRPLTRYLP-VRGAECDLRQHVESAGHQVELC-PHVQITSSSCRGYLHKLGGK-WRSWKKRWFVFDRNRRALIYFSDKTETKLKGGVPFQAIEEVYV-----DHLHSVKSPNPRVTFCV---------KTYERTFHLMAPTAEAMRIWVDVIFTGAEGYLEFL 185
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Match: A0A131XHU4 (Putative pleckstrin similarity domain protein (Fragment) OS=Hyalomma excavatum OX=257692 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 5.931e-24
Identity = 60/169 (35.50%), Postives = 91/169 (53.85%), Query Frame = 2 
Query:  851 QTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            + +   RPLTRYLP       DLR ++E  GH +      +++ S SC G L KL GK  ++WKK+ ++FDRNRR   Y++  T TK+KG + F  I  ++          S+ S +    F +         + ++    L APT+E+ R+W+DVIFTGAE Y+  L+
Sbjct:   35 EKQKAQRPLTRYLP-VRGAECDLRQHVESAGHQVELC-PHVQLTSSSCRGYLHKLGGK-WRSWKKRWFVFDRNRRALMYFSDKTETKLKGGVPFQAIEEVYV-----DHLHSVKSPNPRVTFCV---------KTYERTFHLMAPTAEAMRIWVDVIFTGAEGYLEFLS 186
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Match: A0A131YBE6 (Pleckstrin similarity domain protein OS=Rhipicephalus appendiculatus OX=34631 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 9.731e-24
Identity = 60/169 (35.50%), Postives = 91/169 (53.85%), Query Frame = 2 
Query:  851 QTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            + +   RPLTRYLP       DLR ++E  GH +      +++ S SC G L KL GK  ++WKK+ ++FDRNRR   Y++  T TK+KG + F  I  ++          S+ S +    F +         + ++    L APT+E+ R+W+DVIFTGAE Y+  L+
Sbjct:   46 EKQKAQRPLTRYLPV-RGAECDLRQHVESAGHQVELC-PHVQLTSSSCRGYLHKLGGK-WRSWKKRWFVFDRNRRALMYFSDKTETKLKGGVPFQAIEEVYV-----DHLHSVKSPNPRVTFCV---------KTYERTFHLMAPTAEAMRIWVDVIFTGAEGYLEFLS 197
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Match: A0A0S7JST2 (PHLB2 OS=Poeciliopsis prolifica OX=188132 GN=PHLB2 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 9.837e-24
Identity = 68/170 (40.00%), Postives = 91/170 (53.53%), Query Frame = 2 
Query:  839 KSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNI-FSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            K   + +  SRPLTRYLP   D  FDLR +IE  GHN  +C    L I   +C G L K+ GK +KTWKK+ ++FDRNRR  +YY      K+KG+I F  I  ++          S   S N ++ FS++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct:   66 KMREKQRAQSRPLTRYLPVRKDD-FDLRAHIESAGHNTDTCFH--LSISEKTCRGYLIKMGGK-IKTWKKRWFVFDRNRRTLSYYADKHEAKLKGVIYFQAIEEVYY-----DHLKSAHKSPNPSLTFSVKTHDRV---------YYMVAPSPEAMRIWMDVIVTGAEGY 217
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Match: A0A444TDB1 (Pleckstrin homology-like domain family B member 2 OS=Armadillidium vulgare OX=13347 GN=Avbf_05007 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.760e-23
Identity = 60/169 (35.50%), Postives = 91/169 (53.85%), Query Frame = 2 
Query:  836 SKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            S +  + K   RPLTRYLP  ++  FDLR +IE  GH +    T+L ++  SC G L KL  K  +TW+K+ ++FDR++R F YY+  + +K +G   F  I +++          S+ S      F ++  D            +L AP+ E+ R+W+DVIFTGAE Y
Sbjct:    6 SDTGKKEKTQQRPLTRYLPVRSEEHFDLRQHIESAGHQIELC-TYLTLNHMSCRGYLHKLGSK-FRTWQKRWFVFDRSKRAFLYYSDKSESKPRGGTYFQAIEDVYVDHH------SVKSPSPKLAFCVKTHDR---------TYQLMAPSPEAMRIWIDVIFTGAEGY 157
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 110.153 bits (274), Expect = 5.076e-25
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR ++E  GHN+ +C +  L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  ++        +  + S+     F++ L ++ N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1274 ARPMTRYLPIRKE-EFDLRLHVESSGHNIDTCYQVILT--EKMCKGYLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHETKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPNPSLTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1431
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 110.153 bits (274), Expect = 5.133e-25
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR ++E  GHN+ +C +  L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  ++        +  + S+     F++ L ++ N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1413 ARPMTRYLPIRKE-EFDLRLHVESSGHNIDTCYQVILT--EKMCKGYLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHETKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPNPSLTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1570
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 110.153 bits (274), Expect = 5.176e-25
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR ++E  GHN+ +C +  L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  ++        +  + S+     F++ L ++ N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1409 ARPMTRYLPIRKE-EFDLRLHVESSGHNIDTCYQVILT--EKMCKGYLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHETKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPNPSLTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1566
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 110.153 bits (274), Expect = 5.248e-25
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR ++E  GHN+ +C +  L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  ++        +  + S+     F++ L ++ N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1435 ARPMTRYLPIRKE-EFDLRLHVESSGHNIDTCYQVILT--EKMCKGYLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHETKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPNPSLTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1592
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Match: phldb1b (pleckstrin homology-like domain, family B, member 1b [Source:ZFIN;Acc:ZDB-GENE-121114-8])

HSP 1 Score: 110.153 bits (274), Expect = 5.507e-25
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNF----KDEL-KLSAPTSESQRVWMDVIFTGAEAYMTTLN 1357
            +RP+TRYLP   +  FDLR ++E  GHN+ +C +  L      C G L K+ GK +K+WKK+ ++FDR +R F+YY     TK+KG+I F  I  ++        +  + S+     F++ L ++ N    F     D L  + AP+ E+ R+WMDVI TGAE Y   +N
Sbjct: 1452 ARPMTRYLPIRKE-EFDLRLHVESSGHNIDTCYQVILT--EKMCKGYLVKMGGK-IKSWKKRWFVFDRLKRTFSYYADKHETKLKGVIYFQAIEEVY--------YDHLRSATKKGFFNLNLTNSPNPSLTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN 1609
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Nematostella
Match: EDO43499 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYD3])

HSP 1 Score: 52.373 bits (124), Expect = 9.918e-9
Identity = 30/109 (27.52%), Postives = 50/109 (45.87%), Query Frame = 2 
Query: 1013 LSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEA 1339
            + GK  KTWKK+ ++FDR R+   Y+        KG+I F  I +++A S   +K            F +         +  +    ++AP+  +  +W+DVI TG E 
Sbjct:    1 MGGKIRKTWKKRWFVFDRQRKALLYFHDEHKKDPKGIIYFQAIEDVYAASWCSNK-----GPSPRTTFCV---------KTPQKSYFIAAPSGLAMSIWIDVILTGREG 95
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Match: phldb1a (pleckstrin homology-like domain family B member 1 [Source:NCBI gene;Acc:101154798])

HSP 1 Score: 111.694 bits (278), Expect = 1.771e-25
Identity = 64/163 (39.26%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLPN  +  FDLR ++E CGH+L +C   F+ +    C G L K+ GK +K+WKK+ ++FDR +R F YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1181 ARPMTRYLPNRKE-EFDLRAHVESCGHSLDTC--PFVILTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRNFCYYVDKHETKLKGLIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGY 1323
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Match: phldb1a (pleckstrin homology-like domain family B member 1 [Source:NCBI gene;Acc:101154798])

HSP 1 Score: 111.309 bits (277), Expect = 1.863e-25
Identity = 64/163 (39.26%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLPN  +  FDLR ++E CGH+L +C   F+ +    C G L K+ GK +K+WKK+ ++FDR +R F YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1138 ARPMTRYLPNRKE-EFDLRAHVESCGHSLDTC--PFVILTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRNFCYYVDKHETKLKGLIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGY 1280
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Match: phldb1a (pleckstrin homology-like domain family B member 1 [Source:NCBI gene;Acc:101154798])

HSP 1 Score: 111.309 bits (277), Expect = 1.895e-25
Identity = 64/163 (39.26%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLPN  +  FDLR ++E CGH+L +C   F+ +    C G L K+ GK +K+WKK+ ++FDR +R F YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1196 ARPMTRYLPNRKE-EFDLRAHVESCGHSLDTC--PFVILTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRNFCYYVDKHETKLKGLIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGY 1338
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Match: phldb1a (pleckstrin homology-like domain family B member 1 [Source:NCBI gene;Acc:101154798])

HSP 1 Score: 111.309 bits (277), Expect = 1.933e-25
Identity = 64/163 (39.26%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLPN  +  FDLR ++E CGH+L +C   F+ +    C G L K+ GK +K+WKK+ ++FDR +R F YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1204 ARPMTRYLPNRKE-EFDLRAHVESCGHSLDTC--PFVILTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRNFCYYVDKHETKLKGLIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGY 1346
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Match: phldb1a (pleckstrin homology-like domain family B member 1 [Source:NCBI gene;Acc:101154798])

HSP 1 Score: 111.309 bits (277), Expect = 1.960e-25
Identity = 64/163 (39.26%), Postives = 90/163 (55.21%), Query Frame = 2 
Query:  866 SRPLTRYLPNPNDPRFDLRNYIEMCGHNL-SCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI---HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAY 1342
            +RP+TRYLPN  +  FDLR ++E CGH+L +C   F+ +    C G L K+ GK +K+WKK+ ++FDR +R F YY     TK+KG+I F  I  +   H  S T S   S+        F ++  D             + AP+ E+ R+WMDVI TGAE Y
Sbjct: 1222 ARPMTRYLPNRKE-EFDLRAHVESCGHSLDTC--PFVILTEKMCKGHLVKMGGK-IKSWKKRWFVFDRLKRNFCYYVDKHETKLKGLIYFQAIEEVYYDHLRSATKSPNPSL-------TFCVKTHDRL---------YYMVAPSPEAMRIWMDVIVTGAEGY 1364
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Planmine SMEST
Match: SMESG000033071.1 (SMESG000033071.1)

HSP 1 Score: 806.979 bits (2083), Expect = 0.000e+0
Identity = 401/406 (98.77%), Postives = 406/406 (100.00%), Query Frame = 2 
Query:  185 LQILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIIXXXXXXXXXXXXXXDYKSENSFHNADTNSGTSEKGNNYLKSVVQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIDWTFFPGNFNRNYKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 1402
            ++ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSV+QPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEI+WTFFPGNFNRN+KQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI
Sbjct:   25 IKILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSVIQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIEWTFFPGNFNRNHKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 430
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Planmine SMEST
Match: SMESG000033071.1 (SMESG000033071.1)

HSP 1 Score: 805.053 bits (2078), Expect = 0.000e+0
Identity = 401/404 (99.26%), Postives = 404/404 (100.00%), Query Frame = 2 
Query:  191 ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIIXXXXXXXXXXXXXXDYKSENSFHNADTNSGTSEKGNNYLKSVVQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIDWTFFPGNFNRNYKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 1402
            ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSV+QPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEI+WTFFPGNFNRN+KQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI
Sbjct:    5 ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSVIQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIEWTFFPGNFNRNHKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 408
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Planmine SMEST
Match: SMESG000033071.1 (SMESG000033071.1)

HSP 1 Score: 798.89 bits (2062), Expect = 0.000e+0
Identity = 401/411 (97.57%), Postives = 404/411 (98.30%), Query Frame = 2 
Query:  191 ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIIXXXXXXXXXXXXXXDYKSENSFHNADTNSGTSEKGNNYLKSVVQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIDWTFFPGNFNR-------NYKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 1402
            ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSV+QPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEI+WTFFPGNFNR       N+KQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI
Sbjct:    5 ILETIKQEKKSIEKWSEQVEDLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISSSSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSVIQPRPKSQNYQQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLTQHKTTVRPSSVTSSLCSNPQSTNEIEWTFFPGNFNRLIGLSIKNHKQSASVRSKSSYQTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI 415
[Close]
BLAST of Pleckstrin homology like domain family B member 2 vs. Planmine SMEST
Match: SMESG000057173.1 (SMESG000057173.1)

HSP 1 Score: 100.138 bits (248), Expect = 4.269e-22
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 2 
Query:  815 KQSASVRSKSSYQTKNT--SRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYGKLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNIHA----VSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQRVWMDVIFTGAEAYMT 1348
            + S   R+K  + +K +  SRPLT +LP  +   F+L  ++E CGH+L+     ++ID +SC G LWKLS +    W+K+ + FD   R   YY S + +K+KG I F  I ++       + +P K    L  + H I S  LI                A   E   +W+DVIFTGAEAY+T
Sbjct:  465 RTSVVRRNKDKHISKKSKQSRPLTCFLPKLHSD-FNLLKHVETCGHDLTVCGDHVQIDQNSCQGFLWKLSNRIFGVWRKRWFHFDHKDRQLKYYNSKSKSKIKGCIDFQNISDVFVDHTHRARSPQKLS--LCVETH-IKSYYLI----------------AAKPELVTLWVDVIFTGAEAYLT 628
The following BLAST results are available for this feature:
BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PHLDB21.034e-2437.58pleckstrin homology like domain family B member 2 ... [more]
PHLDB22.890e-2437.58pleckstrin homology like domain family B member 2 ... [more]
PHLDB26.288e-2437.58pleckstrin homology like domain family B member 2 ... [more]
PHLDB26.288e-2437.58pleckstrin homology like domain family B member 2 ... [more]
PHLDB26.845e-2437.58pleckstrin homology like domain family B member 2 ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG50041.972e-1632.28gene:FBgn0260748 transcript:FBtr0346797[more]
CG50043.050e-1632.28gene:FBgn0260748 transcript:FBtr0343201[more]
CG50043.319e-1631.88gene:FBgn0260748 transcript:FBtr0302377[more]
CG50047.291e-1631.88gene:FBgn0260748 transcript:FBtr0302375[more]
CG50047.291e-1631.88gene:FBgn0260748 transcript:FBtr0302376[more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
phldb2a5.701e-2541.61pleckstrin homology-like domain, family B, member ... [more]
phldb2b4.462e-2440.37pleckstrin homology-like domain, family B, member ... [more]
phldb1b8.748e-2436.31pleckstrin homology-like domain, family B, member ... [more]
phldb1b3.297e-2335.88pleckstrin homology-like domain, family B, member ... [more]
phldb1a1.357e-2236.81pleckstrin homology-like domain, family B, member ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdm17.940e-2334.91Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-9... [more]
mdm14.154e-2034.55Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-9... [more]
mdm14.470e-2034.55Mdm1 nuclear protein [Source:Xenbase;Acc:XB-GENE-9... [more]
PHLDB32.176e-1735.42pleckstrin homology-like domain, family B, member ... [more]
PHLDB32.176e-1735.42pleckstrin homology-like domain, family B, member ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Phldb21.474e-2435.16pleckstrin homology like domain, family B, member ... [more]
Phldb16.046e-2437.35pleckstrin homology like domain, family B, member ... [more]
Phldb16.664e-2437.35pleckstrin homology like domain, family B, member ... [more]
Phldb29.692e-2436.97pleckstrin homology like domain, family B, member ... [more]
Phldb21.021e-2336.97pleckstrin homology like domain, family B, member ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q86SQ0|PHLB2_HUMAN3.020e-2337.58Pleckstrin homology-like domain family B member 2 ... [more]
sp|Q8K1N2|PHLB2_MOUSE6.779e-2336.97Pleckstrin homology-like domain family B member 2 ... [more]
sp|Q63312|PHLB1_RAT9.760e-2337.35Pleckstrin homology-like domain family B member 1 ... [more]
sp|Q6PDH0|PHLB1_MOUSE5.019e-2037.35Pleckstrin homology-like domain family B member 1 ... [more]
sp|Q86UU1|PHLB1_HUMAN9.982e-2036.26Pleckstrin homology-like domain family B member 1 ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1E1X9M33.860e-2436.31Putative pleckstrin similarity domain protein (Fra... [more]
A0A131XHU45.931e-2435.50Putative pleckstrin similarity domain protein (Fra... [more]
A0A131YBE69.731e-2435.50Pleckstrin similarity domain protein OS=Rhipicepha... [more]
A0A0S7JST29.837e-2440.00PHLB2 OS=Poeciliopsis prolifica OX=188132 GN=PHLB2... [more]
A0A444TDB11.760e-2335.50Pleckstrin homology-like domain family B member 2 ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
phldb1b5.076e-2537.06pleckstrin homology-like domain, family B, member ... [more]
phldb1b5.133e-2537.06pleckstrin homology-like domain, family B, member ... [more]
phldb1b5.176e-2537.06pleckstrin homology-like domain, family B, member ... [more]
phldb1b5.248e-2537.06pleckstrin homology-like domain, family B, member ... [more]
phldb1b5.507e-2537.06pleckstrin homology-like domain, family B, member ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO434999.918e-927.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
phldb1a1.771e-2539.26pleckstrin homology-like domain family B member 1 ... [more]
phldb1a1.863e-2539.26pleckstrin homology-like domain family B member 1 ... [more]
phldb1a1.895e-2539.26pleckstrin homology-like domain family B member 1 ... [more]
phldb1a1.933e-2539.26pleckstrin homology-like domain family B member 1 ... [more]
phldb1a1.960e-2539.26pleckstrin homology-like domain family B member 1 ... [more]
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BLAST of Pleckstrin homology like domain family B member 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000033071.10.000e+098.77SMESG000033071.1[more]
SMESG000033071.10.000e+099.26SMESG000033071.1[more]
SMESG000033071.10.000e+097.57SMESG000033071.1[more]
SMESG000057173.14.269e-2234.78SMESG000057173.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006683 ID=SMED30006683|Name=Pleckstrin homology like domain family B member 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1472bp
CTCGTCATTCTGAATGCGTCCACTTGATCAAAAATTGCGCTATGAACTTA
GTTAAGAGTTCAAGTTTGAATTTTTGAAAAATTTTAAATATTATATTAAA
ATGATATCGCGTTTTGTTATTGAACAGCACGTAATAGCGTTGTGCAAATT
GATGACTGTCAAATTTAACATAACCAATGATTCTTTGCAGATATTGGAAA
CAATAAAACAAGAAAAGAAGTCAATTGAAAAATGGTCAGAACAAGTGGAA
GATCTGAAACAAACCGATGTCAATAAGGATTTGTGTGACGAATTACAGGA
GAAAATCAAATCCAAATTGCGGTTTATTGAAGATTTGGAATTTGAAGCTT
TAGAGAGTCAAATAGCATTTGAAGAAGGTCAACAGAACATCATTTCAAGT
TCAAGTTCCAGTCCACAATCACATTCCATGGATTCGGATTATAAAAGCGA
GAATTCTTTCCACAATGCTGACACAAACAGTGGAACATCAGAAAAAGGAA
ATAATTATTTAAAGTCTGTCGTTCAACCTCGACCAAAATCACAAAACTAT
CAACAAGAAATCTACTGGCAAAAACCATTTCCGATTCAGCTATGCTTGTA
TGATACTCCACCAGTTTCACATTATGCCAATCAACAGACGAACGGTGAAA
GATCACAGAGCTACTCGAAGACCGATACTGGATATGGGGACACTCTAACT
CAACACAAAACCACTGTTAGGCCGTCCTCAGTAACATCCTCCCTATGTTC
AAATCCCCAGTCGACAAACGAAATCGATTGGACCTTTTTCCCTGGAAATT
TTAACCGGAATTACAAGCAGTCAGCGTCAGTTCGATCTAAATCCAGTTAT
CAAACAAAAAACACATCGAGACCCCTAACGAGATATTTACCCAACCCCAA
TGATCCCAGATTTGATTTGAGAAATTATATAGAAATGTGTGGTCACAATC
TGAGTTGTGTTGAAACCTTCCTGAAAATAGATTCTCATTCGTGTTATGGA
AAGCTCTGGAAACTTTCCGGAAAGCATTTGAAAACCTGGAAAAAGAAGTC
ATATATTTTTGATAGAAATCGACGCATTTTTGCATATTATACCAGTTCGA
CTTCGACAAAAGTTAAAGGAATGATCTCATTTGATCAAATTGTAAATATT
CATGCAGTCTCTGAAACGCCTTCAAAATTCGGATCCATTCTATCATCAGA
CAATCATAATATATTCAGTATACAACTCATCGACAACTGGAACCGAGGCC
GCAATTTCAAGGATGAACTTAAATTGAGTGCCCCAACATCTGAAAGTCAA
AGAGTTTGGATGGATGTAATATTCACAGGGGCCGAGGCTTATATGACAAC
TTTAAATGCCAATCGGACAGATGTATTGCATACCAATAATCAGTGGCTTA
TTTAGAATTAAATTATACATTTTTTGCACAGAAATAATTTTTATTTCATA
GTCAAATATACATTCATATCAA
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protein sequence of SMED30006683-orf-1

>SMED30006683-orf-1 ID=SMED30006683-orf-1|Name=SMED30006683-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=435bp
MISRFVIEQHVIALCKLMTVKFNITNDSLQILETIKQEKKSIEKWSEQVE
DLKQTDVNKDLCDELQEKIKSKLRFIEDLEFEALESQIAFEEGQQNIISS
SSSSPQSHSMDSDYKSENSFHNADTNSGTSEKGNNYLKSVVQPRPKSQNY
QQEIYWQKPFPIQLCLYDTPPVSHYANQQTNGERSQSYSKTDTGYGDTLT
QHKTTVRPSSVTSSLCSNPQSTNEIDWTFFPGNFNRNYKQSASVRSKSSY
QTKNTSRPLTRYLPNPNDPRFDLRNYIEMCGHNLSCVETFLKIDSHSCYG
KLWKLSGKHLKTWKKKSYIFDRNRRIFAYYTSSTSTKVKGMISFDQIVNI
HAVSETPSKFGSILSSDNHNIFSIQLIDNWNRGRNFKDELKLSAPTSESQ
RVWMDVIFTGAEAYMTTLNANRTDVLHTNNQWLI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005925focal adhesion
GO:0005737cytoplasm
GO:0005829cytosol
GO:0005886plasma membrane
GO:0016020membrane
GO:0031252cell leading edge
GO:0045111intermediate filament cytoskeleton
GO:0045180basal cortex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001849PH_domain
IPR011993PH-like_dom_sf
Vocabulary: biological process
TermDefinition
GO:0000226microtubule cytoskeleton organization
GO:0010470regulation of gastrulation
GO:0010717regulation of epithelial to mesenchymal transition
GO:0045184establishment of protein localization
GO:0051497negative regulation of stress fiber assembly
GO:0051895negative regulation of focal adhesion assembly
GO:0070507regulation of microtubule cytoskeleton organization
GO:1903690negative regulation of wound healing, spreading of epidermal cells
GO:1904261positive regulation of basement membrane assembly involved in embryonic body morphogenesis
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0045296cadherin binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 35..55
NoneNo IPR availableCOILSCoilCoilcoord: 62..82
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..113
NoneNo IPR availablePANTHERPTHR12156GRB2-ASSOCIATED BINDER, GABcoord: 247..416
NoneNo IPR availablePANTHERPTHR12156:SF28coord: 247..416
NoneNo IPR availableSUPERFAMILYSSF50729PH domain-likecoord: 297..408
IPR001849Pleckstrin homology domainSMARTSM00233PH_updatecoord: 296..414
e-value: 0.01
score: 25.1
IPR001849Pleckstrin homology domainPFAMPF00169PHcoord: 300..407
e-value: 4.4E-6
score: 27.2
IPR001849Pleckstrin homology domainPROSITEPS50003PH_DOMAINcoord: 295..412
score: 8.444
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 291..409
e-value: 2.3E-10
score: 42.7