SMED30006663

Overview
NameSMED30006663
Smed IDSMED30006663
Length (bp)1622
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006663 (SMED30006663) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006663 (SMED30006663) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006663 (SMED30006663) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006663 (SMED30006663) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006663

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30006663SMESG000029399.1 dd_Smed_v4_1499_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30006663SMESG000029399.1 dd_Smed_v4_1499_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30006663SMESG000029399.1 dd_Smed_v4_1499_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006663SMESG000029399.1 Contig45366newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006663SMESG000029399.1 Contig45366uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
glial cellSMED30006663SMESG000029399.1 dd_Smed_v6_837_1dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30006663 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003334:2109..10838 -3791
Homology
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BLAST of SMED30006663 vs. Planmine SMEST
Match: SMESG000029399.1 (SMESG000029399.1)

HSP 1 Score: 447.973 bits (1151), Expect = 8.184e-157
Identity = 241/254 (94.88%), Postives = 241/254 (94.88%), Query Frame = 2 
Query:   83 MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNXXXXXXXXXXXXXXCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQ------------GVNFKPKGDLGDVSGAHSNA 808
            MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNFIIIFTIIIIAIITCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQ             VNFKPKGDLGDVSGAHSNA
Sbjct:    1 MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNFIIIFTIIIIAIITCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQEVQSLNTYKILCQVNFKPKGDLGDVSGAHSNA 254
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BLAST of SMED30006663 vs. Planmine SMEST
Match: SMESG000029399.1 (SMESG000029399.1)

HSP 1 Score: 417.927 bits (1073), Expect = 2.213e-145
Identity = 223/224 (99.55%), Postives = 223/224 (99.55%), Query Frame = 2 
Query:   83 MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNXXXXXXXXXXXXXXCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQGV 754
            MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNFIIIFTIIIIAIITCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQ V
Sbjct:    1 MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHMNSNFIIIFTIIIIAIITCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVEDAKSDSQNKAQKYQPIPTNELQEV 224
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BLAST of SMED30006663 vs. Planmine SMEST
Match: SMESG000079579.1 (SMESG000079579.1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.594e-13
Identity = 42/143 (29.37%), Postives = 78/143 (54.55%), Query Frame = 2 
Query:   86 VFILLVHAL---LISTSI-SLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLII-TGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPL---------SKIFYNFSYLTIDFNNELDDNITVV 472
            + +L+V+ L   LIS ++  L +N+ NC+NG +L   C F I         + C+L   T +  + F+N    +K Q NN CP F+ + +++++T ++A TCGG IDP+         +++FY+   L     + L+ ++T++
Sbjct:    1 MLLLIVYFLTNFLISNAVKHLKVNQTNCINGADLFHPCGFIIDVKGDINGDIRCQLHFNTSNATVEFSNKIISIKSQANNECPHFSFQFLTSLRTYRLATTCGGHIDPVNQTIKSFISAELFYDLQGLISRNESRLEMSLTIL 143
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BLAST of SMED30006663 vs. Planmine SMEST
Match: SMESG000079624.1 (SMESG000079624.1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.597e-13
Identity = 40/117 (34.19%), Postives = 67/117 (57.26%), Query Frame = 2 
Query:   89 FILLVHALLISTSIS-----LDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLIITGSTNILFN--NTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSKIFYNF 418
             ILLV   LI   IS     + +N+ NC+NG+ L   C F I+      + + C+L    ++N+  N  +  F +KR+ NN+CP F++   ++++T ++A  CGG IDP++K F +F
Sbjct:    1 MILLVGYFLIIFCISNAVKIMKVNQSNCINGSNLIHPCGFLINVKGYINEDILCQLYFK-TSNVTVNVLSRIFYIKRRVNNLCPIFSINFDTSLRTYRLATNCGGHIDPVNKTFESF 116
[Close]
BLAST of SMED30006663 vs. Planmine SMEST
Match: SMESG000079565.1 (SMESG000079565.1)

HSP 1 Score: 67.3958 bits (163), Expect = 4.829e-13
Identity = 37/111 (33.33%), Postives = 59/111 (53.15%), Query Frame = 2 
Query:   74 ICKMVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLNCKLII-TGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGGKIDPLSK 403
            +   +F L  H  L +    L +N+ NC  G  L++ C F I       + + C+L   T    + F+N    +K Q NN CP F+  LV++++T ++A TCGG IDP++K
Sbjct:    6 VSSAIFCLAFH--LSAAVKYLAVNQSNCRIGANLTNPCGFIIDVKGDINEDIRCQLHFNTSDATVEFSNQIIYIKGQANNKCPYFSAHLVTSLRTYRLATTCGGHIDPVNK 114
The following BLAST results are available for this feature:
BLAST of SMED30006663 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006663 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029399.18.184e-15794.88SMESG000029399.1[more]
SMESG000029399.12.213e-14599.55SMESG000029399.1[more]
SMESG000079579.13.594e-1329.37SMESG000079579.1[more]
SMESG000079624.14.597e-1334.19SMESG000079624.1[more]
SMESG000079565.14.829e-1333.33SMESG000079565.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006663 ID=SMED30006663|Name=SMED30006663|organism=Schmidtea mediterranea sexual|type=transcript|length=1622bp
ATAATATAAAGGGAAAGTTTTGGATCCGAATTACCTTTTTTTTCCTAATT
ATATTTTCTCTAGATAAAGTTATATATGTAAAATGGTTTTTATTTTATTA
GTACATGCATTGTTAATTTCCACTTCCATCTCTTTAGATATAAATAAGGA
TAATTGTGTTAATGGAAATGAATTATCTGATAAATGCTCTTTCAATATAT
CATTTAGTAAAATCGGGAGACAAAAATTAAATTGCAAGTTGATTATTACT
GGCAGCACAAATATTCTGTTCAATAATACATTTTTCGATTTGAAACGTCA
AGAAAATAATATGTGCCCGTCATTTACAGTGAAATTGGTCTCAACAATAA
AGACAACAAAGGTAGCCGCTACTTGTGGTGGCAAGATTGATCCATTGAGC
AAAATCTTTTACAACTTCAGCTATCTTACAATTGATTTTAACAATGAATT
AGATGATAATATAACGGTAGTTCAACTCTACTTTGATAGCATTCCCCCAT
GCGAGAAATATATATTTCCAAATATTCATATGAATTCTAATTTCATTATT
ATTTTTACTATTATTATTATTGCTATCATTACTTGTGGGCTTACCGTTAT
GCTTATCGTTTATGTCGGTCACAGAAAGAAGAAAAGTCAATTTACCCAAA
AAGTCAACGAAACTGAACCCAAGAAGGTAGAGGATGCTAAATCAGACAGT
CAAAATAAAGCCCAGAAATATCAACCTATCCCCACCAATGAATTGCAAGG
AGTGAATTTTAAGCCCAAAGGCGATCTTGGAGATGTTTCTGGAGCGCATT
CAAACGCCTAGTTTAACAAAAGGGAGCTACAAAAGTCAATAACAAATCAA
GAATTTGTTTATTTATGTGTTTCATACTTATTAATACATTACTATTATTA
TTAAGTTTCATTTTCTTAATTTATCCACTGTTTTTTTATATGCCTATGAA
GTTTTGTTAATATTTTATTAATAATATTATATTTTAGACAGAATATCGGT
TGAAATTGTCTAAATATTTTGTATACCGTTTTTGTTGTCATTTTTCATTC
ATTTAGAATTATGTGAACTTTTGCTATTAGTTTGCTGCGCCGTAATGATA
TATGTAGTTATTGATGTTCGCGGTTTATTTTCATATATGATTTATCATTG
TCTGATTGGATTTGCAATATGATTTTGATTTTATATAGTAATATATTTAT
AATATTAAAATTTGACAGCAATAAATTGTGAGATAATTATGTATGTATGT
TGTAAAGTATAAATTTGCTCTCTTGTCGTTTGTGATATTTGTGACAATAT
TTTGCACGTGTTTCGTTGTAAAATTAAGTTTATGAAAATTGCGCAAAAAA
ATTTCAATGCTATTAAATCATTTAATTATTCATATGGACACTTTAAATGG
TTAAGAATGCTATTTGGCTTGTGCAATGCATCAGTCTAGTTTTAAAGGAC
CATGTCCAATATTTTTAAGGGTATGGAAAATGAACTTGTCCAATTAATTT
TATTTAGATCAGTATTACCCAACCTTTTCACTGATGAGGTGGCAACTTTG
TTGTTTTGATTTAATGAATTTTATTGTATATAAATGTGCCATCAAAGTTA
AATAAATATGAAGTGATTTATA
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protein sequence of SMED30006663-orf-1

>SMED30006663-orf-1 ID=SMED30006663-orf-1|Name=SMED30006663-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MVFILLVHALLISTSISLDINKDNCVNGNELSDKCSFNISFSKIGRQKLN
CKLIITGSTNILFNNTFFDLKRQENNMCPSFTVKLVSTIKTTKVAATCGG
KIDPLSKIFYNFSYLTIDFNNELDDNITVVQLYFDSIPPCEKYIFPNIHM
NSNFIIIFTIIIIAIITCGLTVMLIVYVGHRKKKSQFTQKVNETEPKKVE
DAKSDSQNKAQKYQPIPTNELQGVNFKPKGDLGDVSGAHSNA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002056medial muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
PLANA:0007528glial cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..242
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.823
NoneNo IPR availableTMHMMTMhelixcoord: 154..176