60S ribosomal protein L32

Overview
Name60S ribosomal protein L32
Smed IDSMED30006655
Length (bp)545
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L32 (SMED30006655) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L32 (SMED30006655) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L32 (SMED30006655) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L32 (SMED30006655) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30006655

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006655SMESG000060036.1 SmedASXL_004464SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30006655SMESG000060036.1 dd_Smed_v4_149_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30006655SMESG000060036.1 dd_Smed_v4_149_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30006655SMESG000060036.1 dd_Smed_v4_149_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006655SMESG000060036.1 dd_Smed_v4_149_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30006655SMESG000060036.1 dd_Smed_v4_149_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30006655SMESG000060036.1 dd_Smed_v6_149_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 140.584 bits (353), Expect = 1.108e-42
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 140.584 bits (353), Expect = 1.108e-42
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 140.584 bits (353), Expect = 1.108e-42
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 140.584 bits (353), Expect = 1.108e-42
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 138.272 bits (347), Expect = 1.028e-41
Identity = 67/133 (50.38%), Postives = 95/133 (71.43%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVE 508
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEE 133          
BLAST of 60S ribosomal protein L32 vs. Ensembl Celegans
Match: rpl-32 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q22716])

HSP 1 Score: 93.5893 bits (231), Expect = 8.993e-25
Identity = 54/114 (47.37%), Postives = 77/114 (67.54%), Query Frame = 2
Query:  173 RHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            RH SDR+ R+   WRKP+GIDNRVRR+F+G   MP IG+G+ +  R V  +G+K   + NV++LD+LLMQS      +   VSAK R  IV+RA +L I++ N  A+++T E+E
Sbjct:   21 RHESDRYRRVAPSWRKPKGIDNRVRRRFRGMRAMPTIGHGSDRRTRFVLPNGYKKVLVQNVKDLDMLLMQSYKYIGEIGHGVSAKSRKGIVERAAQLNIKLTNGNARLRTEESE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Celegans
Match: rpl-32 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q22716])

HSP 1 Score: 93.5893 bits (231), Expect = 8.993e-25
Identity = 54/114 (47.37%), Postives = 77/114 (67.54%), Query Frame = 2
Query:  173 RHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            RH SDR+ R+   WRKP+GIDNRVRR+F+G   MP IG+G+ +  R V  +G+K   + NV++LD+LLMQS      +   VSAK R  IV+RA +L I++ N  A+++T E+E
Sbjct:   21 RHESDRYRRVAPSWRKPKGIDNRVRRRFRGMRAMPTIGHGSDRRTRFVLPNGYKKVLVQNVKDLDMLLMQSYKYIGEIGHGVSAKSRKGIVERAAQLNIKLTNGNARLRTEESE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Celegans
Match: rpl-32 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q22716])

HSP 1 Score: 93.5893 bits (231), Expect = 8.993e-25
Identity = 54/114 (47.37%), Postives = 77/114 (67.54%), Query Frame = 2
Query:  173 RHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            RH SDR+ R+   WRKP+GIDNRVRR+F+G   MP IG+G+ +  R V  +G+K   + NV++LD+LLMQS      +   VSAK R  IV+RA +L I++ N  A+++T E+E
Sbjct:   21 RHESDRYRRVAPSWRKPKGIDNRVRRRFRGMRAMPTIGHGSDRRTRFVLPNGYKKVLVQNVKDLDMLLMQSYKYIGEIGHGVSAKSRKGIVERAAQLNIKLTNGNARLRTEESE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Celegans
Match: rpl-32 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q22716])

HSP 1 Score: 65.4698 bits (158), Expect = 1.693e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 2
Query:  272 MPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            MP IG+G+ +  R V  +G+K   + NV++LD+LLMQS      +   VSAK R  IV+RA +L I++ N  A+++T E+E
Sbjct:    1 MPTIGHGSDRRTRFVLPNGYKKVLVQNVKDLDMLLMQSYKYIGEIGHGVSAKSRKGIVERAAQLNIKLTNGNARLRTEESE 81          
BLAST of 60S ribosomal protein L32 vs. Ensembl Fly
Match: RpL32 (gene:FBgn0002626 transcript:FBtr0085593)

HSP 1 Score: 142.895 bits (359), Expect = 8.377e-44
Identity = 71/137 (51.82%), Postives = 97/137 (70.80%), Query Frame = 2
Query:  104 YKMTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            +KMT I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+R+    +   VS+KKR  IV+RA++L +R+ N   ++++ E E
Sbjct:   12 FKMT-IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHGVSSKKRKEIVERAKQLSVRLTNPNGRLRSQENE 147          
BLAST of 60S ribosomal protein L32 vs. Ensembl Fly
Match: RpL32 (gene:FBgn0002626 transcript:FBtr0085594)

HSP 1 Score: 140.198 bits (352), Expect = 6.473e-43
Identity = 68/132 (51.52%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+R+    +   VS+KKR  IV+RA++L +R+ N   ++++ E E
Sbjct:    3 IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHGVSSKKRKEIVERAKQLSVRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Fly
Match: RpL32 (gene:FBgn0002626 transcript:FBtr0085592)

HSP 1 Score: 140.198 bits (352), Expect = 6.473e-43
Identity = 68/132 (51.52%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+R+    +   VS+KKR  IV+RA++L +R+ N   ++++ E E
Sbjct:    3 IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHGVSSKKRKEIVERAKQLSVRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Fly
Match: RpL32 (gene:FBgn0002626 transcript:FBtr0114555)

HSP 1 Score: 140.198 bits (352), Expect = 6.473e-43
Identity = 68/132 (51.52%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+R+    +   VS+KKR  IV+RA++L +R+ N   ++++ E E
Sbjct:    3 IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHGVSSKKRKEIVERAKQLSVRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Zebrafish
Match: rpl32 (ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-060331-105])

HSP 1 Score: 133.65 bits (335), Expect = 3.992e-40
Identity = 65/135 (48.15%), Postives = 94/135 (69.63%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ +  +++   GFK F + NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIRANWRKPRGIDNRVRRRFKGQMLMPNIGYGSNRKTKHMLPSGFKKFLVHNVKELEVLMMSNKSHCAEIAHNVSSKNRKLIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Zebrafish
Match: rpl32 (ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-060331-105])

HSP 1 Score: 133.65 bits (335), Expect = 3.992e-40
Identity = 65/135 (48.15%), Postives = 94/135 (69.63%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ +  +++   GFK F + NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIRANWRKPRGIDNRVRRRFKGQMLMPNIGYGSNRKTKHMLPSGFKKFLVHNVKELEVLMMSNKSHCAEIAHNVSSKNRKLIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Zebrafish
Match: rpl32 (ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-060331-105])

HSP 1 Score: 133.65 bits (335), Expect = 3.992e-40
Identity = 65/135 (48.15%), Postives = 94/135 (69.63%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ +  +++   GFK F + NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIRANWRKPRGIDNRVRRRFKGQMLMPNIGYGSNRKTKHMLPSGFKKFLVHNVKELEVLMMSNKSHCAEIAHNVSSKNRKLIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Xenopus
Match: hcn3 (hyperpolarization activated cyclic nucleotide gated potassium channel 3 [Source:Xenbase;Acc:XB-GENE-6071710])

HSP 1 Score: 137.502 bits (345), Expect = 1.862e-41
Identity = 66/135 (48.89%), Postives = 93/135 (68.89%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ ++   WRKPRGIDNR RR+FKGQ+ MP IGYG+ K  +++   GFK F + NV+EL+VLLM ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRARRRFKGQILMPNIGYGSNKKTKHMLPTGFKKFLVHNVKELEVLLMSNKTFCAEIAHNVSSKNRKTIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Xenopus
Match: rpl32 (ribosomal protein L32 [Source:NCBI gene;Acc:496894])

HSP 1 Score: 137.502 bits (345), Expect = 1.862e-41
Identity = 66/135 (48.89%), Postives = 93/135 (68.89%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ ++   WRKPRGIDNR RR+FKGQ+ MP IGYG+ K  +++   GFK F + NV+EL+VLLM ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRARRRFKGQILMPNIGYGSNKKTKHMLPTGFKKFLVHNVKELEVLLMSNKTFCAEIAHNVSSKNRKTIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Xenopus
Match: hcn3 (hyperpolarization activated cyclic nucleotide gated potassium channel 3 [Source:Xenbase;Acc:XB-GENE-6071710])

HSP 1 Score: 132.109 bits (331), Expect = 2.569e-39
Identity = 67/141 (47.52%), Postives = 93/141 (65.96%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDR------LGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  I KKRTK++ RH SDR+ +      L   WRKPRGIDNR RR+FKGQ+ MP IGYG+ K  +++   GFK F + NV+EL+VLLM ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIKVCFPLQRNWRKPRGIDNRARRRFKGQILMPNIGYGSNKKTKHMLPTGFKKFLVHNVKELEVLLMSNKTFCAEIAHNVSSKNRKTIVERAAQLAIKVTNPNARLRSEENE 141          
BLAST of 60S ribosomal protein L32 vs. Ensembl Mouse
Match: Rpl32 (ribosomal protein L32 [Source:MGI Symbol;Acc:MGI:98038])

HSP 1 Score: 140.584 bits (353), Expect = 7.607e-43
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Mouse
Match: Rpl32 (ribosomal protein L32 [Source:MGI Symbol;Acc:MGI:98038])

HSP 1 Score: 140.584 bits (353), Expect = 7.607e-43
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GF+ F + NV+EL+VLLM ++   A +   VS+K R AIV+RA +L IRV N  A++++ E E
Sbjct:    1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Match: sp|Q962T1|RL32_SPOFR (60S ribosomal protein L32 OS=Spodoptera frugiperda OX=7108 GN=RpL32 PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 8.471e-44
Identity = 70/132 (53.03%), Postives = 97/132 (73.48%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P+ +  I KKRTKR+ RH SDR+D+L   WRKPRGIDNRVRR+FKGQ  MP IGYG+ K  R++  +GF+   + NV EL++L+MQ+R   A +   VS+KKR  IV+RA++L IRV N+AA++++ E E
Sbjct:    3 IRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGFRKVLVHNVRELEILMMQNRKYCAEIAHGVSSKKRKTIVERAQQLSIRVTNSAARLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Match: sp|P84311|RL32_DROSU (60S ribosomal protein L32 OS=Drosophila subobscura OX=7241 GN=RpL32 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.159e-42
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+RI    +   VS+KKR  IV+RA++L IR+ N   ++++ E E
Sbjct:    3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRIYCGEIAHAVSSKKRKEIVERAKQLSIRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Match: sp|P84313|RL32_DROMD (60S ribosomal protein L32 OS=Drosophila madeirensis OX=30013 GN=RpL32 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.159e-42
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+RI    +   VS+KKR  IV+RA++L IR+ N   ++++ E E
Sbjct:    3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRIYCGEIAHAVSSKKRKEIVERAKQLSIRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Match: sp|P84314|RL32_DROGU (60S ribosomal protein L32 OS=Drosophila guanche OX=7266 GN=RpL32 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.159e-42
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+RI    +   VS+KKR  IV+RA++L IR+ N   ++++ E E
Sbjct:    3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRIYCGEIAHAVSSKKRKEIVERAKQLSIRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Match: sp|P84312|RL32_DROBF (60S ribosomal protein L32 OS=Drosophila bifasciata OX=7218 GN=RpL32 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.159e-42
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+P  +  I KKRTK + RH SDR+ +L HKWRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R++   GFK F + NV EL+VLLMQ+RI    +   VS+KKR  IV+RA++L IR+ N   ++++ E E
Sbjct:    3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKRTRHMLPTGFKKFLVHNVRELEVLLMQNRIYCGEIAHAVSSKKRKEIVERAKQLSIRLTNPNGRLRSQENE 134          
BLAST of 60S ribosomal protein L32 vs. TrEMBL
Match: A0A183R6M6 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 5.409e-50
Identity = 88/137 (64.23%), Postives = 104/137 (75.91%), Query Frame = 2
Query:  104 YKMTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            YKM  I+PL    I KK  K+W RHHSD + RLG KWRKPRGIDNRVRR+FKGQVRMPKIGYGT K  R++H DGFKH  I NV+EL+VLLMQ R  AAV+  TV+ K RV IV+RA++L IRVVNA A +++ E E
Sbjct:   53 YKMA-IRPLHHPRIIKKHPKKWHRHHSDLYKRLGDKWRKPRGIDNRVRRRFKGQVRMPKIGYGTAKRTRHIHPDGFKHVVIRNVKELEVLLMQHRTHAAVIAHTVARKNRVKIVERAKQLSIRVVNANAGLRSTENE 188          
BLAST of 60S ribosomal protein L32 vs. TrEMBL
Match: A0A095C7G4 (60S ribosomal protein L32 OS=Schistosoma haematobium OX=6185 GN=MS3_06453 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.224e-49
Identity = 84/132 (63.64%), Postives = 101/132 (76.52%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+PL    I KK  K+W RHHSD + RLG KWRKPRGIDNRVRR+FKGQVRMPKIGYG+ K  R++H DGFKH  I NV+EL+VLLMQ R  AAV+  TV+ K RV IV+RA++L IRVVNA A +++ E E
Sbjct:    3 IRPLHHPRIIKKHPKKWHRHHSDLYKRLGDKWRKPRGIDNRVRRRFKGQVRMPKIGYGSAKRTRHIHPDGFKHVLIRNVKELEVLLMQHRTHAAVIAHTVARKNRVKIVERAKQLSIRVVNANAGLRSTENE 134          
BLAST of 60S ribosomal protein L32 vs. TrEMBL
Match: A0A430QRT2 (Large subunit ribosomal protein L32e OS=Schistosoma bovis OX=6184 GN=DC041_0012368 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.224e-49
Identity = 84/132 (63.64%), Postives = 101/132 (76.52%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+PL    I KK  K+W RHHSD + RLG KWRKPRGIDNRVRR+FKGQVRMPKIGYG+ K  R++H DGFKH  I NV+EL+VLLMQ R  AAV+  TV+ K RV IV+RA++L IRVVNA A +++ E E
Sbjct:    3 IRPLHHPRIIKKHPKKWHRHHSDLYKRLGDKWRKPRGIDNRVRRRFKGQVRMPKIGYGSAKRTRHIHPDGFKHVLIRNVKELEVLLMQHRTHAAVIAHTVARKNRVKIVERAKQLSIRVVNANAGLRSTENE 134          
BLAST of 60S ribosomal protein L32 vs. TrEMBL
Match: A0A5K4F7C5 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.660e-49
Identity = 84/132 (63.64%), Postives = 101/132 (76.52%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            I+PL    I KK  K+W RHHSD + RLG KWRKPRGIDNRVRR+FKGQVRMPKIGYG+ K  R++H DGFKH  I NV+EL+VLLMQ R  AAV+  TV+ K RV IV+RA++L IRVVNA A +++ E E
Sbjct:    3 IRPLHHPRIIKKHPKKWHRHHSDLYKRLGDKWRKPRGIDNRVRRRFKGQVRMPKIGYGSAKRTRHIHPDGFKHVVIRNVKELEVLLMQHRTHAAVIAHTVARKNRVKIVERAKQLSIRVVNANAGLRSTENE 134          
BLAST of 60S ribosomal protein L32 vs. TrEMBL
Match: A0A0R3X187 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS6932 PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 1.003e-47
Identity = 82/132 (62.12%), Postives = 99/132 (75.00%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            +KPL    I KK  K+W RH SDR+ RLG KWRKP+GIDNRVRR+FKGQ+RMPKIGY T K  R++H DGFKH  I+NV+EL+VL+MQ R  A V+  TVS K RV IV+RA+EL IRVVN  AK++  E E
Sbjct:    3 LKPLPHPKIVKKHPKKWHRHQSDRYVRLGTKWRKPKGIDNRVRRRFKGQLRMPKIGYRTAKRVRHIHPDGFKHVLINNVKELEVLMMQHRTHAGVISRTVSRKNRVKIVERAKELNIRVVNPNAKIRLTENE 134          
BLAST of 60S ribosomal protein L32 vs. Ensembl Cavefish
Match: rpl32 (ribosomal protein L32 [Source:NCBI gene;Acc:103043325])

HSP 1 Score: 139.043 bits (349), Expect = 2.716e-42
Identity = 67/135 (49.63%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K NI KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GFK F ++NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:    1 MPILRPLTKSNIVKKRTKKFIRHQSDRYVKIRKNWRKPRGIDNRVRRRFKGQMLMPNIGYGSNKKTKHMLPTGFKKFLVNNVKELEVLMMSNKSYCAEIAHNVSSKNRKLIVERAAQLAIKVTNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Cavefish
Match: rpl32 (ribosomal protein L32 [Source:NCBI gene;Acc:103043325])

HSP 1 Score: 139.428 bits (350), Expect = 6.647e-42
Identity = 67/135 (49.63%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K NI KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  +++   GFK F ++NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I+V N  A++++ E E
Sbjct:   44 MPILRPLTKSNIVKKRTKKFIRHQSDRYVKIRKNWRKPRGIDNRVRRRFKGQMLMPNIGYGSNKKTKHMLPTGFKKFLVNNVKELEVLMMSNKSYCAEIAHNVSSKNRKLIVERAAQLAIKVTNPNARLRSEENE 178          
BLAST of 60S ribosomal protein L32 vs. Ensembl Sea Lamprey
Match: RPL32 (ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC:10336])

HSP 1 Score: 142.124 bits (357), Expect = 3.137e-44
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL+K  I KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+RMP IGYG+ K  + +   GF+ F + NV EL+VL+M ++   A +   VS+KKR  IV+RA +L I+V NA A++++ E E
Sbjct:    1 MVALRPLVKPKIVKKRTKKFKRHQSDRYIKIKPNWRKPRGIDNRVRRRFKGQLRMPNIGYGSNKKTKYMLPTGFRKFLVHNVRELEVLMMSNKTFCAEIAHNVSSKKRKTIVERAAQLAIKVTNANARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Ensembl Yeast
Match: RPL32 (Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog [Source:SGD;Acc:S000000188])

HSP 1 Score: 111.694 bits (278), Expect = 1.278e-32
Identity = 53/113 (46.90%), Postives = 77/113 (68.14%), Query Frame = 2
Query:  143 IEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVN 481
            I KK TK++ RHHSDR+ R+   WRK +GID+ VRR+F+G +  PKIGYG+ K  + +   G K F ++NV++L+ L M ++  AA +   +SAK RV I+ RA+ LGI+V N
Sbjct:    9 IVKKHTKKFKRHHSDRYHRVAENWRKQKGIDSVVRRRFRGNISQPKIGYGSNKKTKFLSPSGHKTFLVANVKDLETLTMHTKTYAAEIAHNISAKNRVVILARAKALGIKVTN 121          
BLAST of 60S ribosomal protein L32 vs. Ensembl Nematostella
Match: EDO44975 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU93])

HSP 1 Score: 140.969 bits (354), Expect = 1.480e-43
Identity = 68/132 (51.52%), Postives = 96/132 (72.73%), Query Frame = 2
Query:  119 IKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            + P+ +  I KKR K++ RH SDR+ R+G  WRKP+GIDNRVRR+FKGQ  MP IGYG+ K  R +  DGFK F + NV+EL+VL+M +R  AA +   VS++KR AIV+RA++L I+V N+ A++++ E E
Sbjct:    2 VMPVNRPRILKKRQKKFIRHQSDRYMRVGESWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKKTRFLMPDGFKKFVVHNVKELEVLMMMNRSYAAEIAHNVSSRKRKAIVERAQQLSIKVTNSNARLRSEENE 133          
BLAST of 60S ribosomal protein L32 vs. Ensembl Medaka
Match: rpl32 (ribosomal protein L32 [Source:NCBI gene;Acc:110015361])

HSP 1 Score: 134.42 bits (337), Expect = 1.909e-40
Identity = 64/135 (47.41%), Postives = 93/135 (68.89%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            M  ++PL K  + KKRTK++ RH SDR+ ++   WRKPRGIDNRVRR+FKGQ+ MP IGYG+ K  + +   GFK F + NV+EL+VL+M ++   A +   VS+K R  IV+RA +L I++ N  A++++ E E
Sbjct:    1 MAALRPLTKPRLIKKRTKKFIRHQSDRYVKIKQNWRKPRGIDNRVRRRFKGQMLMPNIGYGSNKKTKFILPTGFKKFLVHNVKELEVLMMSNKTHCAEIAHNVSSKNRKVIVERAAQLAIKITNPNARLRSEENE 135          
BLAST of 60S ribosomal protein L32 vs. Planmine SMEST
Match: SMESG000060036.1 (SMESG000060036.1)

HSP 1 Score: 275.404 bits (703), Expect = 3.102e-96
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 2
Query:  110 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 514
            MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE
Sbjct:    1 MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVDSTVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE 135          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL321.108e-4250.37ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL321.108e-4250.37ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL321.108e-4250.37ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL321.108e-4250.37ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL321.028e-4150.38ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rpl-328.993e-2547.37Ribosomal Protein, Large subunit [Source:UniProtK... [more]
rpl-328.993e-2547.37Ribosomal Protein, Large subunit [Source:UniProtK... [more]
rpl-328.993e-2547.37Ribosomal Protein, Large subunit [Source:UniProtK... [more]
rpl-321.693e-1441.98Ribosomal Protein, Large subunit [Source:UniProtK... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RpL328.377e-4451.82gene:FBgn0002626 transcript:FBtr0085593[more]
RpL326.473e-4351.52gene:FBgn0002626 transcript:FBtr0085594[more]
RpL326.473e-4351.52gene:FBgn0002626 transcript:FBtr0085592[more]
RpL326.473e-4351.52gene:FBgn0002626 transcript:FBtr0114555[more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpl323.992e-4048.15ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-06... [more]
rpl323.992e-4048.15ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-06... [more]
rpl323.992e-4048.15ribosomal protein L32 [Source:ZFIN;Acc:ZDB-GENE-06... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
hcn31.862e-4148.89hyperpolarization activated cyclic nucleotide gate... [more]
rpl321.862e-4148.89ribosomal protein L32 [Source:NCBI gene;Acc:496894... [more]
hcn32.569e-3947.52hyperpolarization activated cyclic nucleotide gate... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rpl327.607e-4350.37ribosomal protein L32 [Source:MGI Symbol;Acc:MGI:9... [more]
Rpl327.607e-4350.37ribosomal protein L32 [Source:MGI Symbol;Acc:MGI:9... [more]
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BLAST of 60S ribosomal protein L32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q962T1|RL32_SPOFR8.471e-4453.0360S ribosomal protein L32 OS=Spodoptera frugiperda... [more]
sp|P84311|RL32_DROSU1.159e-4253.0360S ribosomal protein L32 OS=Drosophila subobscura... [more]
sp|P84313|RL32_DROMD1.159e-4253.0360S ribosomal protein L32 OS=Drosophila madeirensi... [more]
sp|P84314|RL32_DROGU1.159e-4253.0360S ribosomal protein L32 OS=Drosophila guanche OX... [more]
sp|P84312|RL32_DROBF1.159e-4253.0360S ribosomal protein L32 OS=Drosophila bifasciata... [more]
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BLAST of 60S ribosomal protein L32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183R6M65.409e-5064.23Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A095C7G44.224e-4963.6460S ribosomal protein L32 OS=Schistosoma haematobi... [more]
A0A430QRT24.224e-4963.64Large subunit ribosomal protein L32e OS=Schistosom... [more]
A0A5K4F7C54.660e-4963.64Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A0R3X1871.003e-4762.12Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl322.716e-4249.63ribosomal protein L32 [Source:NCBI gene;Acc:103043... [more]
rpl326.647e-4249.63ribosomal protein L32 [Source:NCBI gene;Acc:103043... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RPL323.137e-4450.37ribosomal protein L32 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RPL321.278e-3246.90Ribosomal 60S subunit protein L32; overexpression ... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO449751.480e-4351.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 60S ribosomal protein L32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl321.909e-4047.41ribosomal protein L32 [Source:NCBI gene;Acc:110015... [more]
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BLAST of 60S ribosomal protein L32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000060036.13.102e-96100.00SMESG000060036.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006655 ID=SMED30006655|Name=60S ribosomal protein L32|organism=Schmidtea mediterranea sexual|type=transcript|length=545bp
AAAGTTGCTTACAAATTTTACTAGCAATAATTATTTACATAATAAAATTT
AAATTTTTGTTTAAATTTGGTATTTTATTTAAAGTCGAATTATTTAAATC
TTTTACAAGATGACCCAAATTAAACCTCTTATTAAACACAATATTGAAAA
GAAGCGTACTAAAAGATGGACCAGACATCATTCTGATAGATTTGATAGAT
TGGGACATAAATGGAGAAAACCAAGGGGTATTGATAACCGTGTTCGACGA
AAGTTTAAAGGCCAAGTTAGGATGCCTAAAATTGGTTACGGTACAAGAAA
ATGTTATAGAAATGTTCACAAGGACGGATTCAAACATTTCCCTATTTCAA
ACGTAGAGGAACTTGATGTTCTTCTCATGCAAAGCCGTATTGTAGCAGCA
GTTGTTGATTCAACTGTTTCTGCAAAGAAACGAGTTGCAATTGTTAAACG
TGCTGAGGAATTGGGAATAAGAGTTGTCAATGCAGCTGCCAAAGTTAAAA
CTGTCGAAACCGAATAAAAAAGTTAAAATAAAAATTTTTGGATTT
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protein sequence of SMED30006655-orf-1

>SMED30006655-orf-1 ID=SMED30006655-orf-1|Name=SMED30006655-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp
MTQIKPLIKHNIEKKRTKRWTRHHSDRFDRLGHKWRKPRGIDNRVRRKFK
GQVRMPKIGYGTRKCYRNVHKDGFKHFPISNVEELDVLLMQSRIVAAVVD
STVSAKKRVAIVKRAEELGIRVVNAAAKVKTVETE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036351Ribosomal_L32e_sf
IPR001515Ribosomal_L32e
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001515Ribosomal protein L32eSMARTSM01393Ribosomal_L32e_2coord: 17..124
e-value: 7.0E-54
score: 195.0
IPR001515Ribosomal protein L32ePFAMPF01655Ribosomal_L32ecoord: 17..124
e-value: 3.6E-42
score: 143.2
IPR001515Ribosomal protein L32ePANTHERPTHR2341360S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT Ncoord: 5..134
IPR001515Ribosomal protein L32eCDDcd00513Ribosomal_L32_L32ecoord: 18..124
e-value: 4.55006E-47
score: 145.75
NoneNo IPR availablePRODOMPD003823coord: 18..124
e-value: 3.0E-36
score: 375.0
IPR036351Ribosomal protein L32e superfamilySUPERFAMILYSSF52042Ribosomal protein L32ecoord: 2..131