E3 ubiquitin protein ligase HUWE1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006629 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Match: HUWE1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:30892]) HSP 1 Score: 583.948 bits (1504), Expect = 2.963e-177 Identity = 297/532 (55.83%), Postives = 370/532 (69.55%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+L++ S +W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 669 LLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 1196
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Match: HUWE1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:30892]) HSP 1 Score: 593.578 bits (1529), Expect = 4.863e-169 Identity = 297/532 (55.83%), Postives = 370/532 (69.55%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+L++ S +W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3846 LLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4373 HSP 2 Score: 385.956 bits (990), Expect = 4.809e-106 Identity = 236/677 (34.86%), Postives = 364/677 (53.77%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 77.0258 bits (188), Expect = 1.142e-12 Identity = 81/300 (27.00%), Postives = 139/300 (46.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEH-------RRA---NRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
+ EG+DP+FL ALP +RREV+ R A N + G V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL + S
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSS 3234 HSP 4 Score: 67.3958 bits (163), Expect = 9.660e-10 Identity = 78/303 (25.74%), Postives = 123/303 (40.59%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP--------------------SKSKDQRKFENL---------SEYEHKIISCVPDPLGDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SK D ++ EN S K S +G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 4.822e-7 Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 3
Query: 2511 KDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 745 QQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Match: HUWE1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:30892]) HSP 1 Score: 593.578 bits (1529), Expect = 4.863e-169 Identity = 297/532 (55.83%), Postives = 370/532 (69.55%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+L++ S +W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3846 LLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4373 HSP 2 Score: 385.956 bits (990), Expect = 4.809e-106 Identity = 236/677 (34.86%), Postives = 364/677 (53.77%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 77.0258 bits (188), Expect = 1.142e-12 Identity = 81/300 (27.00%), Postives = 139/300 (46.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEH-------RRA---NRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
+ EG+DP+FL ALP +RREV+ R A N + G V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL + S
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSS 3234 HSP 4 Score: 67.3958 bits (163), Expect = 9.660e-10 Identity = 78/303 (25.74%), Postives = 123/303 (40.59%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP--------------------SKSKDQRKFENL---------SEYEHKIISCVPDPLGDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SK D ++ EN S K S +G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 4.822e-7 Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 3
Query: 2511 KDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 745 QQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Match: HUWE1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:30892]) HSP 1 Score: 593.193 bits (1528), Expect = 5.981e-169 Identity = 297/532 (55.83%), Postives = 370/532 (69.55%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+L++ S +W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3837 LLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4364 HSP 2 Score: 385.956 bits (990), Expect = 4.306e-106 Identity = 236/677 (34.86%), Postives = 364/677 (53.77%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 76.6406 bits (187), Expect = 1.338e-12 Identity = 81/300 (27.00%), Postives = 139/300 (46.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEH-------RRA---NRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
+ EG+DP+FL ALP +RREV+ R A N + G V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL + S
Sbjct: 2955 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSS 3225 HSP 4 Score: 67.3958 bits (163), Expect = 9.740e-10 Identity = 78/303 (25.74%), Postives = 123/303 (40.59%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP--------------------SKSKDQRKFENL---------SEYEHKIISCVPDPLGDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SK D ++ EN S K S +G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1548 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1841 HSP 5 Score: 58.151 bits (139), Expect = 4.862e-7 Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 3
Query: 2511 KDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 745 QQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Match: ITCH (itchy E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:13890]) HSP 1 Score: 332.028 bits (850), Expect = 4.238e-96 Identity = 170/418 (40.67%), Postives = 253/418 (60.53%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ +++ +I R ALD A ++ DF K +YF+ QL + + + V+R LFEDS+ ++ + ++ ++R + F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ +N++ L+ FS+ G+ ++ DL PNG ILVTEENK EY+R+V + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + + +Y Y S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 345 FVDHNRRTTTYIDPRTGKSALDNGPQIAYVR-----DFKAKVQYFRFWCQQL-----AMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 750
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Match: eel-1 (Enhancer of EfL-1 mutant phenotype [Source:UniProtKB/TrEMBL;Acc:U4PBK1]) HSP 1 Score: 534.643 bits (1376), Expect = 1.260e-151 Identity = 266/521 (51.06%), Postives = 353/521 (67.75%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHN--PFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLR--NDGDSRYEEE--LVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I + S S+W+ LS+ L + K+ +P AV+ Q E+F L H+ S+ ++ K + + + +++ + ++ E+ + +EF EKH++VLN LR+ A F+ L ++PKLLDFD+KR+YF+ ++ +L N RY VQVSR R+F DS+ L R EWK RF I F EEGQD GGLLREW+ ++ EIFNPNYALF +P D VTY IN ASY+N HL YFKFVGR IAK++F++K L CYFTRAFYKHIL + +RY DLE ED FF+ L+FLL+N + + LD TFS ++ EFG RDL PNG +I V + NK EYV+LVCQ KMTG+IR+QL +FL GFYEIIPK+LIS+FNE+ELELLI GLP +D++D+ NT Y + S IQWFW+AL SF++ED+A+F+QFVTGTSKVPL GF+ LEGMNG KF I + S + LPAAHTCFN +DLPQYE+++KL + LL AIREC+EG+G
Sbjct: 3662 ISERLSSLESLWNSLSECLLRLGKASDPHAVLALQPAAEAFFLVHA--SQQNKLKAKDAEAKRKESQAASSSAAAAVASSVSHDGLREDLDPDTAKLIEFAEKHRQVLNQALRQNNAVLSAGGPFAILTQFPKLLDFDVKRKYFRKELTKLEPSNSRMPRYRRNDVSVQVSRNRVFSDSFRELFRLRPSEWKNRFYIIFQGEEGQDAGGLLREWFSVITREIFNPNYALFITAPGDMVTYMINKASYINPEHLDYFKFVGRLIAKSVFEHKYLDCYFTRAFYKHILNLPVRYQDLESEDPAFFKSLDFLLQNPIDDLALDLTFSTEVEEFGVRSVRDLKPNGRKIEVNDANKDEYVKLVCQMKMTGSIRKQLDAFLTGFYEIIPKDLISMFNEQELELLISGLPTVDIDDMAANTDYKGFQKTSTHIQWFWRALRSFEKEDKAKFLQFVTGTSKVPLQGFASLEGMNGVQKFSIHMDSRGGDRLPAAHTCFNQLDLPQYESYEKLRQSLLLAIRECTEGFG 4180 HSP 2 Score: 134.035 bits (336), Expect = 2.247e-30 Identity = 137/492 (27.85%), Postives = 219/492 (44.51%), Query Frame = 3
Query: 96 LAALGENDLINEIKKINVWH--YGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFS-RLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTE-YMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMA-----------LFARLRLCVYFGKPEQRKLLIYTRMQALSILCY-----TNEVEDKCLSWD-IVEELVDVLKLPDRE---------FLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKF----QPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC 1469
+A + D I I K H GK EL W +VL+R D IL + V K L+ D + L +AI+ FT L E + R +Y S + ILAL +++MD+L +L L KRS + S R+ + + +RL +IA+ WGGK +AEC E +P M + F D+ Q ++ + +PK + ES EL+ + + + +SD + L +R+R+ V F R + R+ ++S L Y T+E L + +EE+V++LK DRE I+ AL TL +I+ K +L+ ++ SYHG M R+ + +L + +P S TA+ SF+YHLA T LVG+GL ++ +I +H + I+F TR R+ D L ++ +S + ++ V+R+ EI C
Sbjct: 29 VATKNDEDFIAAINKGREVHAVMGKTELYKWTEVLNRCDEILEKAVHKNEFGNLQCDH--DTVLKNHAVAIVRFTVLLFECTSSRRIYKSVDRILALLESTDMDLLAEVLRLLQVMGKRSKFLSTRIPQKEQHALAQRLTAIAQCWGGKLRTVKMAECLKREPKLP-----MLFPFTYT----------DAKQRTI-IVEQPKFQKD-----------ESVGELITRTVAQISPPVSSDSVPSQQFSKEDLYCLLSRVRMLVTFEDWHHRFKCLIVRLLSVSTLVYCRLGGTDETTLSSLLYSGFIEEIVEMLK-SDREADTQQDHNLHDAIQTEALSTLCSIVTYEKEPKITQILEALSA-GSYHGFLSVMTRQIVDELKSNNLGKTGKPSVS-----LATALFSFIYHLASIEPGGDT----LVGSGLTQTLLSVIGFHELPIECITFGTRCARIID---LFTTIDVTSFKANNGMEICVNRVVHEINEC 477 HSP 3 Score: 93.5893 bits (231), Expect = 4.468e-18 Identity = 49/151 (32.45%), Postives = 84/151 (55.63%), Query Frame = 3
Query: 1521 QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMM 1973
QR G IK +L IKR I D ++ + +K M+G+LP+ L I N ++P L S +ITNFVY P +S++Q R + I ++L + LP ++D + TL + +A+ +N RG+ + K ++ + + +F+ +R KR E+
Sbjct: 576 QRSGLIKGLLTFIKRAIGDVQFQDIMKHLMEGDLPEALMHILSN-AEYYSPSLFHQSAHLITNFVYQFPEELSSIQRRHVPYVIFQSLLRKELPNSKDVITTLGNVFTAMCLNERGLRQFKSYDPFNQIFRIVLSVKFLVTLRKKRSAEVF 725 HSP 4 Score: 60.077 bits (144), Expect = 6.113e-8 Identity = 51/183 (27.87%), Postives = 81/183 (44.26%), Query Frame = 3
Query: 8451 LIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ------------------------QLTG---------IQLPENVDAEIMAQLPAHIQEEILA-HYRRENSTSET---ANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQM 8888
+I +G+DP FL ALP+ MR EVI +++R R + Q G + L E +D + LP +QEE+LA H RR E NAP P++ V + SL + R ++LA+MD +++A L +++ +EAR+ R + + M
Sbjct: 2975 IIPDGVDPAFLAALPEEMRAEVIRDYQRQQRAERASRPVPAAQPVAVNPNAPAGGEAAQGEGQAEQPAAAVVPLVEPIDPVFLNALPPELQEEVLAEHERRLREAEEQQRRQNAP--------PAPVVEMDGAAV----IASLPANERAQVLAEMDDAELAGLPADMQNEARRARAQHVEPNM 3145
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Match: eel-1 (Enhancer of EfL-1 mutant phenotype [Source:UniProtKB/TrEMBL;Acc:U4PBK1]) HSP 1 Score: 534.643 bits (1376), Expect = 1.262e-151 Identity = 266/521 (51.06%), Postives = 353/521 (67.75%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHN--PFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLR--NDGDSRYEEE--LVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I + S S+W+ LS+ L + K+ +P AV+ Q E+F L H+ S+ ++ K + + + +++ + ++ E+ + +EF EKH++VLN LR+ A F+ L ++PKLLDFD+KR+YF+ ++ +L N RY VQVSR R+F DS+ L R EWK RF I F EEGQD GGLLREW+ ++ EIFNPNYALF +P D VTY IN ASY+N HL YFKFVGR IAK++F++K L CYFTRAFYKHIL + +RY DLE ED FF+ L+FLL+N + + LD TFS ++ EFG RDL PNG +I V + NK EYV+LVCQ KMTG+IR+QL +FL GFYEIIPK+LIS+FNE+ELELLI GLP +D++D+ NT Y + S IQWFW+AL SF++ED+A+F+QFVTGTSKVPL GF+ LEGMNG KF I + S + LPAAHTCFN +DLPQYE+++KL + LL AIREC+EG+G
Sbjct: 3665 ISERLSSLESLWNSLSECLLRLGKASDPHAVLALQPAAEAFFLVHA--SQQNKLKAKDAEAKRKESQAASSSAAAAVASSVSHDGLREDLDPDTAKLIEFAEKHRQVLNQALRQNNAVLSAGGPFAILTQFPKLLDFDVKRKYFRKELTKLEPSNSRMPRYRRNDVSVQVSRNRVFSDSFRELFRLRPSEWKNRFYIIFQGEEGQDAGGLLREWFSVITREIFNPNYALFITAPGDMVTYMINKASYINPEHLDYFKFVGRLIAKSVFEHKYLDCYFTRAFYKHILNLPVRYQDLESEDPAFFKSLDFLLQNPIDDLALDLTFSTEVEEFGVRSVRDLKPNGRKIEVNDANKDEYVKLVCQMKMTGSIRKQLDAFLTGFYEIIPKDLISMFNEQELELLISGLPTVDIDDMAANTDYKGFQKTSTHIQWFWRALRSFEKEDKAKFLQFVTGTSKVPLQGFASLEGMNGVQKFSIHMDSRGGDRLPAAHTCFNQLDLPQYESYEKLRQSLLLAIRECTEGFG 4183 HSP 2 Score: 134.42 bits (337), Expect = 2.084e-30 Identity = 137/492 (27.85%), Postives = 219/492 (44.51%), Query Frame = 3
Query: 96 LAALGENDLINEIKKINVWH--YGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFS-RLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTE-YMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMA-----------LFARLRLCVYFGKPEQRKLLIYTRMQALSILCY-----TNEVEDKCLSWD-IVEELVDVLKLPDRE---------FLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKF----QPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC 1469
+A + D I I K H GK EL W +VL+R D IL + V K L+ D + L +AI+ FT L E + R +Y S + ILAL +++MD+L +L L KRS + S R+ + + +RL +IA+ WGGK +AEC E +P M + F D+ Q ++ + +PK + ES EL+ + + + +SD + L +R+R+ V F R + R+ ++S L Y T+E L + +EE+V++LK DRE I+ AL TL +I+ K +L+ ++ SYHG M R+ + +L + +P S TA+ SF+YHLA T LVG+GL ++ +I +H + I+F TR R+ D L ++ +S + ++ V+R+ EI C
Sbjct: 29 VATKNDEDFIAAINKGREVHAVMGKTELYKWTEVLNRCDEILEKAVHKNEFGNLQCDH--DTVLKNHAVAIVRFTVLLFECTSSRRIYKSVDRILALLESTDMDLLAEVLRLLQVMGKRSKFLSTRIPQKEQHALAQRLTAIAQCWGGKLRTVKMAECLKREPKLP-----MLFPFTYT----------DAKQRTI-IVEQPKFQKD-----------ESVGELITRTVAQISPPVSSDSVPSQQFSKEDLYCLLSRVRMLVTFEDWHHRFKCLIVRLLSVSTLVYCRLGGTDETTLSSLLYSGFIEEIVEMLK-SDREADTQQDHNLHDAIQTEALSTLCSIVTYEKEPKITQILEALSA-GSYHGFLSVMTRQIVDELKSNNLGKTGKPSVS-----LATALFSFIYHLASIEPGGDT----LVGSGLTQTLLSVIGFHELPIECITFGTRCARIID---LFTTIDVTSFKANNGMEICVNRVVHEINEC 477 HSP 3 Score: 93.5893 bits (231), Expect = 4.358e-18 Identity = 49/151 (32.45%), Postives = 84/151 (55.63%), Query Frame = 3
Query: 1521 QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMM 1973
QR G IK +L IKR I D ++ + +K M+G+LP+ L I N ++P L S +ITNFVY P +S++Q R + I ++L + LP ++D + TL + +A+ +N RG+ + K ++ + + +F+ +R KR E+
Sbjct: 576 QRSGLIKGLLTFIKRAIGDVQFQDIMKHLMEGDLPEALMHILSN-AEYYSPSLFHQSAHLITNFVYQFPEELSSIQRRHVPYVIFQSLLRKELPNSKDVITTLGNVFTAMCLNERGLRQFKSYDPFNQIFRIVLSVKFLVTLRKKRSAEVF 725 HSP 4 Score: 60.077 bits (144), Expect = 6.113e-8 Identity = 51/183 (27.87%), Postives = 81/183 (44.26%), Query Frame = 3
Query: 8451 LIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ------------------------QLTG---------IQLPENVDAEIMAQLPAHIQEEILA-HYRRENSTSET---ANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQM 8888
+I +G+DP FL ALP+ MR EVI +++R R + Q G + L E +D + LP +QEE+LA H RR E NAP P++ V + SL + R ++LA+MD +++A L +++ +EAR+ R + + M
Sbjct: 2978 IIPDGVDPAFLAALPEEMRAEVIRDYQRQQRAERASRPVPAAQPVAVNPNAPAGGEAAQGEGQAEQPAAAVVPLVEPIDPVFLNALPPELQEEVLAEHERRLREAEEQQRRQNAP--------PAPVVEMDGAAV----IASLPANERAQVLAEMDDAELAGLPADMQNEARRARAQHVEPNM 3148
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Match: eel-1 (Enhancer of EfL-1 mutant phenotype [Source:UniProtKB/TrEMBL;Acc:U4PBK1]) HSP 1 Score: 534.643 bits (1376), Expect = 1.282e-151 Identity = 266/521 (51.06%), Postives = 353/521 (67.75%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHN--PFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLR--NDGDSRYEEE--LVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I + S S+W+ LS+ L + K+ +P AV+ Q E+F L H+ S+ ++ K + + + +++ + ++ E+ + +EF EKH++VLN LR+ A F+ L ++PKLLDFD+KR+YF+ ++ +L N RY VQVSR R+F DS+ L R EWK RF I F EEGQD GGLLREW+ ++ EIFNPNYALF +P D VTY IN ASY+N HL YFKFVGR IAK++F++K L CYFTRAFYKHIL + +RY DLE ED FF+ L+FLL+N + + LD TFS ++ EFG RDL PNG +I V + NK EYV+LVCQ KMTG+IR+QL +FL GFYEIIPK+LIS+FNE+ELELLI GLP +D++D+ NT Y + S IQWFW+AL SF++ED+A+F+QFVTGTSKVPL GF+ LEGMNG KF I + S + LPAAHTCFN +DLPQYE+++KL + LL AIREC+EG+G
Sbjct: 3663 ISERLSSLESLWNSLSECLLRLGKASDPHAVLALQPAAEAFFLVHA--SQQNKLKAKDAEAKRKESQAASSSAAAAVASSVSHDGLREDLDPDTAKLIEFAEKHRQVLNQALRQNNAVLSAGGPFAILTQFPKLLDFDVKRKYFRKELTKLEPSNSRMPRYRRNDVSVQVSRNRVFSDSFRELFRLRPSEWKNRFYIIFQGEEGQDAGGLLREWFSVITREIFNPNYALFITAPGDMVTYMINKASYINPEHLDYFKFVGRLIAKSVFEHKYLDCYFTRAFYKHILNLPVRYQDLESEDPAFFKSLDFLLQNPIDDLALDLTFSTEVEEFGVRSVRDLKPNGRKIEVNDANKDEYVKLVCQMKMTGSIRKQLDAFLTGFYEIIPKDLISMFNEQELELLISGLPTVDIDDMAANTDYKGFQKTSTHIQWFWRALRSFEKEDKAKFLQFVTGTSKVPLQGFASLEGMNGVQKFSIHMDSRGGDRLPAAHTCFNQLDLPQYESYEKLRQSLLLAIRECTEGFG 4181 HSP 2 Score: 133.265 bits (334), Expect = 3.938e-30 Identity = 137/492 (27.85%), Postives = 219/492 (44.51%), Query Frame = 3
Query: 96 LAALGENDLINEIKKINVWH--YGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFS-RLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTE-YMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMA-----------LFARLRLCVYFGKPEQRKLLIYTRMQALSILCY-----TNEVEDKCLSWD-IVEELVDVLKLPDRE---------FLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKF----QPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC 1469
+A + D I I K H GK EL W +VL+R D IL + V K L+ D + L +AI+ FT L E + R +Y S + ILAL +++MD+L +L L KRS + S R+ + + +RL +IA+ WGGK +AEC E +P M + F D+ Q ++ + +PK + ES EL+ + + + +SD + L +R+R+ V F R + R+ ++S L Y T+E L + +EE+V++LK DRE I+ AL TL +I+ K +L+ ++ SYHG M R+ + +L + +P S TA+ SF+YHLA T LVG+GL ++ +I +H + I+F TR R+ D L ++ +S + ++ V+R+ EI C
Sbjct: 29 VATKNDEDFIAAINKGREVHAVMGKTELYKWTEVLNRCDEILEKAVHKNEFGNLQCDH--DTVLKNHAVAIVRFTVLLFECTSSRRIYKSVDRILALLESTDMDLLAEVLRLLQVMGKRSKFLSTRIPQKEQHALAQRLTAIAQCWGGKLRTVKMAECLKREPKLP-----MLFPFTYT----------DAKQRTI-IVEQPK-------------KDESVGELITRTVAQISPPVSSDSVPSQQFSKEDLYCLLSRVRMLVTFEDWHHRFKCLIVRLLSVSTLVYCRLGGTDETTLSSLLYSGFIEEIVEMLK-SDREADTQQDHNLHDAIQTEALSTLCSIVTYEKEPKITQILEALSA-GSYHGFLSVMTRQIVDELKSNNLGKTGKPSVS-----LATALFSFIYHLASIEPGGDT----LVGSGLTQTLLSVIGFHELPIECITFGTRCARIID---LFTTIDVTSFKANNGMEICVNRVVHEINEC 475 HSP 3 Score: 93.5893 bits (231), Expect = 4.544e-18 Identity = 49/151 (32.45%), Postives = 84/151 (55.63%), Query Frame = 3
Query: 1521 QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMM 1973
QR G IK +L IKR I D ++ + +K M+G+LP+ L I N ++P L S +ITNFVY P +S++Q R + I ++L + LP ++D + TL + +A+ +N RG+ + K ++ + + +F+ +R KR E+
Sbjct: 574 QRSGLIKGLLTFIKRAIGDVQFQDIMKHLMEGDLPEALMHILSN-AEYYSPSLFHQSAHLITNFVYQFPEELSSIQRRHVPYVIFQSLLRKELPNSKDVITTLGNVFTAMCLNERGLRQFKSYDPFNQIFRIVLSVKFLVTLRKKRSAEVF 723 HSP 4 Score: 60.077 bits (144), Expect = 5.961e-8 Identity = 51/183 (27.87%), Postives = 81/183 (44.26%), Query Frame = 3
Query: 8451 LIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ------------------------QLTG---------IQLPENVDAEIMAQLPAHIQEEILA-HYRRENSTSET---ANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQM 8888
+I +G+DP FL ALP+ MR EVI +++R R + Q G + L E +D + LP +QEE+LA H RR E NAP P++ V + SL + R ++LA+MD +++A L +++ +EAR+ R + + M
Sbjct: 2976 IIPDGVDPAFLAALPEEMRAEVIRDYQRQQRAERASRPVPAAQPVAVNPNAPAGGEAAQGEGQAEQPAAAVVPLVEPIDPVFLNALPPELQEEVLAEHERRLREAEEQQRRQNAP--------PAPVVEMDGAAV----IASLPANERAQVLAEMDDAELAGLPADMQNEARRARAQHVEPNM 3146
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Match: eel-1 (Enhancer of EfL-1 mutant phenotype [Source:UniProtKB/TrEMBL;Acc:U4PBK1]) HSP 1 Score: 534.643 bits (1376), Expect = 1.302e-151 Identity = 266/521 (51.06%), Postives = 353/521 (67.75%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHN--PFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLR--NDGDSRYEEE--LVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I + S S+W+ LS+ L + K+ +P AV+ Q E+F L H+ S+ ++ K + + + +++ + ++ E+ + +EF EKH++VLN LR+ A F+ L ++PKLLDFD+KR+YF+ ++ +L N RY VQVSR R+F DS+ L R EWK RF I F EEGQD GGLLREW+ ++ EIFNPNYALF +P D VTY IN ASY+N HL YFKFVGR IAK++F++K L CYFTRAFYKHIL + +RY DLE ED FF+ L+FLL+N + + LD TFS ++ EFG RDL PNG +I V + NK EYV+LVCQ KMTG+IR+QL +FL GFYEIIPK+LIS+FNE+ELELLI GLP +D++D+ NT Y + S IQWFW+AL SF++ED+A+F+QFVTGTSKVPL GF+ LEGMNG KF I + S + LPAAHTCFN +DLPQYE+++KL + LL AIREC+EG+G
Sbjct: 3660 ISERLSSLESLWNSLSECLLRLGKASDPHAVLALQPAAEAFFLVHA--SQQNKLKAKDAEAKRKESQAASSSAAAAVASSVSHDGLREDLDPDTAKLIEFAEKHRQVLNQALRQNNAVLSAGGPFAILTQFPKLLDFDVKRKYFRKELTKLEPSNSRMPRYRRNDVSVQVSRNRVFSDSFRELFRLRPSEWKNRFYIIFQGEEGQDAGGLLREWFSVITREIFNPNYALFITAPGDMVTYMINKASYINPEHLDYFKFVGRLIAKSVFEHKYLDCYFTRAFYKHILNLPVRYQDLESEDPAFFKSLDFLLQNPIDDLALDLTFSTEVEEFGVRSVRDLKPNGRKIEVNDANKDEYVKLVCQMKMTGSIRKQLDAFLTGFYEIIPKDLISMFNEQELELLISGLPTVDIDDMAANTDYKGFQKTSTHIQWFWRALRSFEKEDKAKFLQFVTGTSKVPLQGFASLEGMNGVQKFSIHMDSRGGDRLPAAHTCFNQLDLPQYESYEKLRQSLLLAIRECTEGFG 4178 HSP 2 Score: 133.265 bits (334), Expect = 3.905e-30 Identity = 137/492 (27.85%), Postives = 219/492 (44.51%), Query Frame = 3
Query: 96 LAALGENDLINEIKKINVWH--YGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFS-RLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTE-YMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMA-----------LFARLRLCVYFGKPEQRKLLIYTRMQALSILCY-----TNEVEDKCLSWD-IVEELVDVLKLPDRE---------FLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKF----QPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC 1469
+A + D I I K H GK EL W +VL+R D IL + V K L+ D + L +AI+ FT L E + R +Y S + ILAL +++MD+L +L L KRS + S R+ + + +RL +IA+ WGGK +AEC E +P M + F D+ Q ++ + +PK + ES EL+ + + + +SD + L +R+R+ V F R + R+ ++S L Y T+E L + +EE+V++LK DRE I+ AL TL +I+ K +L+ ++ SYHG M R+ + +L + +P S TA+ SF+YHLA T LVG+GL ++ +I +H + I+F TR R+ D L ++ +S + ++ V+R+ EI C
Sbjct: 29 VATKNDEDFIAAINKGREVHAVMGKTELYKWTEVLNRCDEILEKAVHKNEFGNLQCDH--DTVLKNHAVAIVRFTVLLFECTSSRRIYKSVDRILALLESTDMDLLAEVLRLLQVMGKRSKFLSTRIPQKEQHALAQRLTAIAQCWGGKLRTVKMAECLKREPKLP-----MLFPFTYT----------DAKQRTI-IVEQPK-------------KDESVGELITRTVAQISPPVSSDSVPSQQFSKEDLYCLLSRVRMLVTFEDWHHRFKCLIVRLLSVSTLVYCRLGGTDETTLSSLLYSGFIEEIVEMLK-SDREADTQQDHNLHDAIQTEALSTLCSIVTYEKEPKITQILEALSA-GSYHGFLSVMTRQIVDELKSNNLGKTGKPSVS-----LATALFSFIYHLASIEPGGDT----LVGSGLTQTLLSVIGFHELPIECITFGTRCARIID---LFTTIDVTSFKANNGMEICVNRVVHEINEC 475 HSP 3 Score: 93.5893 bits (231), Expect = 4.659e-18 Identity = 49/151 (32.45%), Postives = 84/151 (55.63%), Query Frame = 3
Query: 1521 QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMM 1973
QR G IK +L IKR I D ++ + +K M+G+LP+ L I N ++P L S +ITNFVY P +S++Q R + I ++L + LP ++D + TL + +A+ +N RG+ + K ++ + + +F+ +R KR E+
Sbjct: 574 QRSGLIKGLLTFIKRAIGDVQFQDIMKHLMEGDLPEALMHILSN-AEYYSPSLFHQSAHLITNFVYQFPEELSSIQRRHVPYVIFQSLLRKELPNSKDVITTLGNVFTAMCLNERGLRQFKSYDPFNQIFRIVLSVKFLVTLRKKRSAEVF 723 HSP 4 Score: 60.077 bits (144), Expect = 5.961e-8 Identity = 51/183 (27.87%), Postives = 81/183 (44.26%), Query Frame = 3
Query: 8451 LIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ------------------------QLTG---------IQLPENVDAEIMAQLPAHIQEEILA-HYRRENSTSET---ANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQM 8888
+I +G+DP FL ALP+ MR EVI +++R R + Q G + L E +D + LP +QEE+LA H RR E NAP P++ V + SL + R ++LA+MD +++A L +++ +EAR+ R + + M
Sbjct: 2973 IIPDGVDPAFLAALPEEMRAEVIRDYQRQQRAERASRPVPAAQPVAVNPNAPAGGEAAQGEGQAEQPAAAVVPLVEPIDPVFLNALPPELQEEVLAEHERRLREAEEQQRRQNAP--------PAPVVEMDGAAV----IASLPANERAQVLAEMDDAELAGLPADMQNEARRARAQHVEPNM 3143
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Match: eel-1 (Enhancer of EfL-1 mutant phenotype [Source:UniProtKB/TrEMBL;Acc:U4PBK1]) HSP 1 Score: 534.258 bits (1375), Expect = 1.732e-151 Identity = 264/518 (50.97%), Postives = 352/518 (67.95%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHN--PFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDS-RYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I + S S+W+ LS+ L + K+ +P AV+ Q E+F L H+ S+ ++ K + + + +++ + ++ E+ + +EF EKH++VLN LR+ A F+ L ++PKLLDFD+KR+YF+ ++ +L R + VQVSR R+F DS+ L R EWK RF I F EEGQD GGLLREW+ ++ EIFNPNYALF +P D VTY IN ASY+N HL YFKFVGR IAK++F++K L CYFTRAFYKHIL + +RY DLE ED FF+ L+FLL+N + + LD TFS ++ EFG RDL PNG +I V + NK EYV+LVCQ KMTG+IR+QL +FL GFYEIIPK+LIS+FNE+ELELLI GLP +D++D+ NT Y + S IQWFW+AL SF++ED+A+F+QFVTGTSKVPL GF+ LEGMNG KF I + S + LPAAHTCFN +DLPQYE+++KL + LL AIREC+EG+G
Sbjct: 3660 ISERLSSLESLWNSLSECLLRLGKASDPHAVLALQPAAEAFFLVHA--SQQNKLKAKDAEAKRKESQAASSSAAAAVASSVSHDGLREDLDPDTAKLIEFAEKHRQVLNQALRQNNAVLSAGGPFAILTQFPKLLDFDVKRKYFRKELTKLEPSMPRYRRNDVSVQVSRNRVFSDSFRELFRLRPSEWKNRFYIIFQGEEGQDAGGLLREWFSVITREIFNPNYALFITAPGDMVTYMINKASYINPEHLDYFKFVGRLIAKSVFEHKYLDCYFTRAFYKHILNLPVRYQDLESEDPAFFKSLDFLLQNPIDDLALDLTFSTEVEEFGVRSVRDLKPNGRKIEVNDANKDEYVKLVCQMKMTGSIRKQLDAFLTGFYEIIPKDLISMFNEQELELLISGLPTVDIDDMAANTDYKGFQKTSTHIQWFWRALRSFEKEDKAKFLQFVTGTSKVPLQGFASLEGMNGVQKFSIHMDSRGGDRLPAAHTCFNQLDLPQYESYEKLRQSLLLAIRECTEGFG 4175 HSP 2 Score: 133.265 bits (334), Expect = 4.105e-30 Identity = 137/492 (27.85%), Postives = 219/492 (44.51%), Query Frame = 3
Query: 96 LAALGENDLINEIKKINVWH--YGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFS-RLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTE-YMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMA-----------LFARLRLCVYFGKPEQRKLLIYTRMQALSILCY-----TNEVEDKCLSWD-IVEELVDVLKLPDRE---------FLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKF----QPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC 1469
+A + D I I K H GK EL W +VL+R D IL + V K L+ D + L +AI+ FT L E + R +Y S + ILAL +++MD+L +L L KRS + S R+ + + +RL +IA+ WGGK +AEC E +P M + F D+ Q ++ + +PK + ES EL+ + + + +SD + L +R+R+ V F R + R+ ++S L Y T+E L + +EE+V++LK DRE I+ AL TL +I+ K +L+ ++ SYHG M R+ + +L + +P S TA+ SF+YHLA T LVG+GL ++ +I +H + I+F TR R+ D L ++ +S + ++ V+R+ EI C
Sbjct: 29 VATKNDEDFIAAINKGREVHAVMGKTELYKWTEVLNRCDEILEKAVHKNEFGNLQCDH--DTVLKNHAVAIVRFTVLLFECTSSRRIYKSVDRILALLESTDMDLLAEVLRLLQVMGKRSKFLSTRIPQKEQHALAQRLTAIAQCWGGKLRTVKMAECLKREPKLP-----MLFPFTYT----------DAKQRTI-IVEQPK-------------KDESVGELITRTVAQISPPVSSDSVPSQQFSKEDLYCLLSRVRMLVTFEDWHHRFKCLIVRLLSVSTLVYCRLGGTDETTLSSLLYSGFIEEIVEMLK-SDREADTQQDHNLHDAIQTEALSTLCSIVTYEKEPKITQILEALSA-GSYHGFLSVMTRQIVDELKSNNLGKTGKPSVS-----LATALFSFIYHLASIEPGGDT----LVGSGLTQTLLSVIGFHELPIECITFGTRCARIID---LFTTIDVTSFKANNGMEICVNRVVHEINEC 475 HSP 3 Score: 93.5893 bits (231), Expect = 4.581e-18 Identity = 49/151 (32.45%), Postives = 84/151 (55.63%), Query Frame = 3
Query: 1521 QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMM 1973
QR G IK +L IKR I D ++ + +K M+G+LP+ L I N ++P L S +ITNFVY P +S++Q R + I ++L + LP ++D + TL + +A+ +N RG+ + K ++ + + +F+ +R KR E+
Sbjct: 574 QRSGLIKGLLTFIKRAIGDVQFQDIMKHLMEGDLPEALMHILSN-AEYYSPSLFHQSAHLITNFVYQFPEELSSIQRRHVPYVIFQSLLRKELPNSKDVITTLGNVFTAMCLNERGLRQFKSYDPFNQIFRIVLSVKFLVTLRKKRSAEVF 723 HSP 4 Score: 60.077 bits (144), Expect = 5.961e-8 Identity = 51/183 (27.87%), Postives = 81/183 (44.26%), Query Frame = 3
Query: 8451 LIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ------------------------QLTG---------IQLPENVDAEIMAQLPAHIQEEILA-HYRRENSTSET---ANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQM 8888
+I +G+DP FL ALP+ MR EVI +++R R + Q G + L E +D + LP +QEE+LA H RR E NAP P++ V + SL + R ++LA+MD +++A L +++ +EAR+ R + + M
Sbjct: 2973 IIPDGVDPAFLAALPEEMRAEVIRDYQRQQRAERASRPVPAAQPVAVNPNAPAGGEAAQGEGQAEQPAAAVVPLVEPIDPVFLNALPPELQEEVLAEHERRLREAEEQQRRQNAP--------PAPVVEMDGAAV----IASLPANERAQVLAEMDDAELAGLPADMQNEARRARAQHVEPNM 3143
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Match: HUWE1 (gene:FBgn0030674 transcript:FBtr0339689) HSP 1 Score: 572.007 bits (1473), Expect = 8.847e-163 Identity = 296/572 (51.75%), Postives = 375/572 (65.56%), Query Frame = 3
Query: 10647 KVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKS-------------------------------------DNSNVSDPLS------------HLNTSLVVD---DTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
K L+Q +W+ LS+ L A+++SK+ AV+V Q VE+F L H+ ++ +S D S+ + P S NT LV + T M++ FL+F EKH+ VLN ILR+ + L F+ L+ + ++LDFD+KR+YFQ ++ +L D R EE V V R +FEDS+ L+R EEWK RF I F +EEGQD GGLLREWY+ +S EIFNP YALF VSP D VTY INP+S+ N NHLSYFKFVGR IAKA+ DNKLL CYFTR+FYKHILG +++TD+E +DY+F++GL++L+KND+ST+ + TFS ++ EFG T+ RDL PNG VTEENK EYV+LVCQ KM+G+IRQQL +FLEGFY+IIPK+LISIFNE+ELELLI GLP+ID+ DLK NT Y+KYT S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF LEGMNG KFQI ST+ LP AHTCFN +DLP Y+++DKL CLL AI ECSEG+G
Sbjct: 4557 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQ-SPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL--DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5125 HSP 2 Score: 202.216 bits (513), Expect = 7.572e-51 Identity = 150/504 (29.76%), Postives = 248/504 (49.21%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAAL-GENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVG--HWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLH--LQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVE---DKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIH-------CGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEID 1487
MK+D K+ EC +L L +L+ + ++VW YGKCEL W ++LDR D IL E V + L+ D E + ++ +L FT LIEHS R+LY S EH+ L + NMDI+ A+L L Y FSKRSN+ RL +++++ +L +IAE WG +G L +CC+ G + EF+ Q +D ++ H +L + ++ S E+++ + + + + + +M + R+RL F + R + R+QA+SIL Y+N ++ DK L EEL +++ D + I+A LRTLT+++H + S + + TG G P ++R+ I +L + T++ S LYHLA + E+ T LV +G++ S+ +I W + ++I+F TRAVRV D I ++ + +Q + ++ +DRL+ EIK C + D
Sbjct: 1 MKVDTVKLKKGSSELTAECRELIDELTKRRTRAELLEFLDTVHVWIYGKCELYHWIEILDRFDKILEEASRHVNGNEFVLQCDTNFQETDVTLLLHVLNFTTLLIEHSFSRHLYNSIEHLTVLLSSQNMDIVLAVLNLLYMFSKRSNFIPRLPFEKKELLIVKLFNIAERWGDPNYGLSLKDCCI--------GEPKLEFLY--------------QLCID-YVDEHGHAAQLEIPDMMDLCHTASAPEVIKTIAGQISKPSEAIKMRIAHRVRLISGFNNYKLRLQFVQARLQAVSILIYSNALQDNTDKVLYAGFGEELCELIDKEDVHLVEIRAAVLRTLTSMLHFDRNPNVPSRAGSRLMKIVHYTGAERQGGTLPLLVRDCIDNLTTHGL---TERYPLILATSLFSLLYHLA-SYEMGGT---ALVKSGMMQSLLCVISWPGVDLEHITFVTRAVRVIDLIT---NIDIARFHQNNGLNVFIDRLEKEIKSCCKALAD 471 HSP 3 Score: 95.5153 bits (236), Expect = 1.393e-18 Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 3
Query: 1455 EIKICLDSEI-DQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
E K L S++ + K + TQR +KSMLN +K+ I D+ ++ M+ L LR I N + P L L + +V+T +V+N PS +S++Q+ +T +L+ L ++ +P R+ L +LP + SAL +N RG+ E DK +K L ++ AMR +R
Sbjct: 599 ERKAELSSQLPSSLAPKKPSCVTQRAALLKSMLNFLKKSIQDHAHFSNMRNIMETSLTQSLRHIIAN-AEYYGPSLFLLATDVVTVYVFNEPSLLSSLQDLGITSVMLKALLQKDVPATREVLGSLPNVFSALCLNERGLFEFLSYDPFDKVLKVLLSPDYLVAMRRRR 766 HSP 4 Score: 92.0485 bits (227), Expect = 1.381e-17 Identity = 90/300 (30.00%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ--QLTGIQLPEN----VDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDL---LSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKP-----------LVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLK 9293
+ EG+DP+FL ALP MR EVI EH R R+RQ Q IQ+ + V+ E +A LP +IQ E+L R E + A NPE V ++F +L +LR+ IL DM++SQIA+L ELA EA+ +R+ D + R R A ++ LS L ++ RW +I Y S Q H + + A PN+ KP L+ D L L+ L + M++ ++++++L H R WI +++I+ K
Sbjct: 3647 VPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIE----QQRQAAQTANPED----------PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRR---DWESRN--------GARMDAHPPSNALSRFQTTLQSLDDTRW-HSSIWYDA-SGNAQPQHHQHTTIVHQHPHAHHAPNMG--KPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRL-----HRVIRNLCHHEPTRDWIISALISIIQK 3912
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Match: HUWE1 (gene:FBgn0030674 transcript:FBtr0339690) HSP 1 Score: 572.007 bits (1473), Expect = 9.487e-163 Identity = 296/572 (51.75%), Postives = 375/572 (65.56%), Query Frame = 3
Query: 10647 KVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKS-------------------------------------DNSNVSDPLS------------HLNTSLVVD---DTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
K L+Q +W+ LS+ L A+++SK+ AV+V Q VE+F L H+ ++ +S D S+ + P S NT LV + T M++ FL+F EKH+ VLN ILR+ + L F+ L+ + ++LDFD+KR+YFQ ++ +L D R EE V V R +FEDS+ L+R EEWK RF I F +EEGQD GGLLREWY+ +S EIFNP YALF VSP D VTY INP+S+ N NHLSYFKFVGR IAKA+ DNKLL CYFTR+FYKHILG +++TD+E +DY+F++GL++L+KND+ST+ + TFS ++ EFG T+ RDL PNG VTEENK EYV+LVCQ KM+G+IRQQL +FLEGFY+IIPK+LISIFNE+ELELLI GLP+ID+ DLK NT Y+KYT S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF LEGMNG KFQI ST+ LP AHTCFN +DLP Y+++DKL CLL AI ECSEG+G
Sbjct: 4531 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQ-SPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL--DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5099 HSP 2 Score: 202.601 bits (514), Expect = 6.783e-51 Identity = 150/504 (29.76%), Postives = 248/504 (49.21%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAAL-GENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVG--HWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLH--LQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVE---DKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIH-------CGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEID 1487
MK+D K+ EC +L L +L+ + ++VW YGKCEL W ++LDR D IL E V + L+ D E + ++ +L FT LIEHS R+LY S EH+ L + NMDI+ A+L L Y FSKRSN+ RL +++++ +L +IAE WG +G L +CC+ G + EF+ Q +D ++ H +L + ++ S E+++ + + + + + +M + R+RL F + R + R+QA+SIL Y+N ++ DK L EEL +++ D + I+A LRTLT+++H + S + + TG G P ++R+ I +L + T++ S LYHLA + E+ T LV +G++ S+ +I W + ++I+F TRAVRV D I ++ + +Q + ++ +DRL+ EIK C + D
Sbjct: 1 MKVDTVKLKKGSSELTAECRELIDELTKRRTRAELLEFLDTVHVWIYGKCELYHWIEILDRFDKILEEASRHVNGNEFVLQCDTNFQETDVTLLLHVLNFTTLLIEHSFSRHLYNSIEHLTVLLSSQNMDIVLAVLNLLYMFSKRSNFIPRLPFEKKELLIVKLFNIAERWGDPNYGLSLKDCCI--------GEPKLEFLY--------------QLCID-YVDEHGHAAQLEIPDMMDLCHTASAPEVIKTIAGQISKPSEAIKMRIAHRVRLISGFNNYKLRLQFVQARLQAVSILIYSNALQDNTDKVLYAGFGEELCELIDKEDVHLVEIRAAVLRTLTSMLHFDRNPNVPSRAGSRLMKIVHYTGAERQGGTLPLLVRDCIDNLTTHGL---TERYPLILATSLFSLLYHLA-SYEMGGT---ALVKSGMMQSLLCVISWPGVDLEHITFVTRAVRVIDLIT---NIDIARFHQNNGLNVFIDRLEKEIKSCCKALAD 471 HSP 3 Score: 95.5153 bits (236), Expect = 1.428e-18 Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 3
Query: 1455 EIKICLDSEI-DQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
E K L S++ + K + TQR +KSMLN +K+ I D+ ++ M+ L LR I N + P L L + +V+T +V+N PS +S++Q+ +T +L+ L ++ +P R+ L +LP + SAL +N RG+ E DK +K L ++ AMR +R
Sbjct: 599 ERKAELSSQLPSSLAPKKPSCVTQRAALLKSMLNFLKKSIQDHAHFSNMRNIMETSLTQSLRHIIAN-AEYYGPSLFLLATDVVTVYVFNEPSLLSSLQDLGITSVMLKALLQKDVPATREVLGSLPNVFSALCLNERGLFEFLSYDPFDKVLKVLLSPDYLVAMRRRR 766 HSP 4 Score: 92.0485 bits (227), Expect = 1.464e-17 Identity = 90/300 (30.00%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ--QLTGIQLPEN----VDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDL---LSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKP-----------LVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLK 9293
+ EG+DP+FL ALP MR EVI EH R R+RQ Q IQ+ + V+ E +A LP +IQ E+L R E + A NPE V ++F +L +LR+ IL DM++SQIA+L ELA EA+ +R+ D + R R A ++ LS L ++ RW +I Y S Q H + + A PN+ KP L+ D L L+ L + M++ ++++++L H R WI +++I+ K
Sbjct: 3621 VPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIE----QQRQAAQTANPED----------PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRR---DWESRN--------GARMDAHPPSNALSRFQTTLQSLDDTRW-HSSIWYDA-SGNAQPQHHQHTTIVHQHPHAHHAPNMG--KPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRL-----HRVIRNLCHHEPTRDWIISALISIIQK 3886
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Match: HUWE1 (gene:FBgn0030674 transcript:FBtr0339688) HSP 1 Score: 572.007 bits (1473), Expect = 1.010e-162 Identity = 296/572 (51.75%), Postives = 375/572 (65.56%), Query Frame = 3
Query: 10647 KVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKS-------------------------------------DNSNVSDPLS------------HLNTSLVVD---DTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
K L+Q +W+ LS+ L A+++SK+ AV+V Q VE+F L H+ ++ +S D S+ + P S NT LV + T M++ FL+F EKH+ VLN ILR+ + L F+ L+ + ++LDFD+KR+YFQ ++ +L D R EE V V R +FEDS+ L+R EEWK RF I F +EEGQD GGLLREWY+ +S EIFNP YALF VSP D VTY INP+S+ N NHLSYFKFVGR IAKA+ DNKLL CYFTR+FYKHILG +++TD+E +DY+F++GL++L+KND+ST+ + TFS ++ EFG T+ RDL PNG VTEENK EYV+LVCQ KM+G+IRQQL +FLEGFY+IIPK+LISIFNE+ELELLI GLP+ID+ DLK NT Y+KYT S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF LEGMNG KFQI ST+ LP AHTCFN +DLP Y+++DKL CLL AI ECSEG+G
Sbjct: 4546 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQ-SPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL--DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5114 HSP 2 Score: 202.216 bits (513), Expect = 8.161e-51 Identity = 150/504 (29.76%), Postives = 248/504 (49.21%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAAL-GENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVG--HWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLH--LQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVE---DKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIH-------CGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEID 1487
MK+D K+ EC +L L +L+ + ++VW YGKCEL W ++LDR D IL E V + L+ D E + ++ +L FT LIEHS R+LY S EH+ L + NMDI+ A+L L Y FSKRSN+ RL +++++ +L +IAE WG +G L +CC+ G + EF+ Q +D ++ H +L + ++ S E+++ + + + + + +M + R+RL F + R + R+QA+SIL Y+N ++ DK L EEL +++ D + I+A LRTLT+++H + S + + TG G P ++R+ I +L + T++ S LYHLA + E+ T LV +G++ S+ +I W + ++I+F TRAVRV D I ++ + +Q + ++ +DRL+ EIK C + D
Sbjct: 1 MKVDTVKLKKGSSELTAECRELIDELTKRRTRAELLEFLDTVHVWIYGKCELYHWIEILDRFDKILEEASRHVNGNEFVLQCDTNFQETDVTLLLHVLNFTTLLIEHSFSRHLYNSIEHLTVLLSSQNMDIVLAVLNLLYMFSKRSNFIPRLPFEKKELLIVKLFNIAERWGDPNYGLSLKDCCI--------GEPKLEFLY--------------QLCID-YVDEHGHAAQLEIPDMMDLCHTASAPEVIKTIAGQISKPSEAIKMRIAHRVRLISGFNNYKLRLQFVQARLQAVSILIYSNALQDNTDKVLYAGFGEELCELIDKEDVHLVEIRAAVLRTLTSMLHFDRNPNVPSRAGSRLMKIVHYTGAERQGGTLPLLVRDCIDNLTTHGL---TERYPLILATSLFSLLYHLA-SYEMGGT---ALVKSGMMQSLLCVISWPGVDLEHITFVTRAVRVIDLIT---NIDIARFHQNNGLNVFIDRLEKEIKSCCKALAD 471 HSP 3 Score: 95.5153 bits (236), Expect = 1.417e-18 Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 3
Query: 1455 EIKICLDSEI-DQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
E K L S++ + K + TQR +KSMLN +K+ I D+ ++ M+ L LR I N + P L L + +V+T +V+N PS +S++Q+ +T +L+ L ++ +P R+ L +LP + SAL +N RG+ E DK +K L ++ AMR +R
Sbjct: 599 ERKAELSSQLPSSLAPKKPSCVTQRAALLKSMLNFLKKSIQDHAHFSNMRNIMETSLTQSLRHIIAN-AEYYGPSLFLLATDVVTVYVFNEPSLLSSLQDLGITSVMLKALLQKDVPATREVLGSLPNVFSALCLNERGLFEFLSYDPFDKVLKVLLSPDYLVAMRRRR 766 HSP 4 Score: 92.0485 bits (227), Expect = 1.452e-17 Identity = 90/300 (30.00%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ--QLTGIQLPEN----VDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDL---LSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKP-----------LVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLK 9293
+ EG+DP+FL ALP MR EVI EH R R+RQ Q IQ+ + V+ E +A LP +IQ E+L R E + A NPE V ++F +L +LR+ IL DM++SQIA+L ELA EA+ +R+ D + R R A ++ LS L ++ RW +I Y S Q H + + A PN+ KP L+ D L L+ L + M++ ++++++L H R WI +++I+ K
Sbjct: 3636 VPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIE----QQRQAAQTANPED----------PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRR---DWESRN--------GARMDAHPPSNALSRFQTTLQSLDDTRW-HSSIWYDA-SGNAQPQHHQHTTIVHQHPHAHHAPNMG--KPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRL-----HRVIRNLCHHEPTRDWIISALISIIQK 3901
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Match: HUWE1 (gene:FBgn0030674 transcript:FBtr0340304) HSP 1 Score: 572.007 bits (1473), Expect = 1.088e-162 Identity = 296/572 (51.75%), Postives = 375/572 (65.56%), Query Frame = 3
Query: 10647 KVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKS-------------------------------------DNSNVSDPLS------------HLNTSLVVD---DTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
K L+Q +W+ LS+ L A+++SK+ AV+V Q VE+F L H+ ++ +S D S+ + P S NT LV + T M++ FL+F EKH+ VLN ILR+ + L F+ L+ + ++LDFD+KR+YFQ ++ +L D R EE V V R +FEDS+ L+R EEWK RF I F +EEGQD GGLLREWY+ +S EIFNP YALF VSP D VTY INP+S+ N NHLSYFKFVGR IAKA+ DNKLL CYFTR+FYKHILG +++TD+E +DY+F++GL++L+KND+ST+ + TFS ++ EFG T+ RDL PNG VTEENK EYV+LVCQ KM+G+IRQQL +FLEGFY+IIPK+LISIFNE+ELELLI GLP+ID+ DLK NT Y+KYT S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF LEGMNG KFQI ST+ LP AHTCFN +DLP Y+++DKL CLL AI ECSEG+G
Sbjct: 4581 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQ-SPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL--DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149 HSP 2 Score: 202.601 bits (514), Expect = 6.411e-51 Identity = 150/503 (29.82%), Postives = 248/503 (49.30%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAAL-GENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVG--HWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLH--LQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVE---DKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIH------CGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEID 1487
MK+D K+ EC +L L +L+ + ++VW YGKCEL W ++LDR D IL E V + L+ D E + ++ +L FT LIEHS R+LY S EH+ L + NMDI+ A+L L Y FSKRSN+ RL +++++ +L +IAE WG +G L +CC+ G + EF+ Q +D ++ H +L + ++ S E+++ + + + + + +M + R+RL F + R + R+QA+SIL Y+N ++ DK L EEL +++ D + I+A LRTLT+++H + S + + TG G P ++R+ I +L + T++ S LYHLA + E+ T LV +G++ S+ +I W + ++I+F TRAVRV D I ++ + +Q + ++ +DRL+ EIK C + D
Sbjct: 1 MKVDTVKLKKGSSELTAECRELIDELTKRRTRAELLEFLDTVHVWIYGKCELYHWIEILDRFDKILEEASRHVNGNEFVLQCDTNFQETDVTLLLHVLNFTTLLIEHSFSRHLYNSIEHLTVLLSSQNMDIVLAVLNLLYMFSKRSNFIPRLPFEKKELLIVKLFNIAERWGDPNYGLSLKDCCI--------GEPKLEFLY--------------QLCID-YVDEHGHAAQLEIPDMMDLCHTASAPEVIKTIAGQISKPSEAIKMRIAHRVRLISGFNNYKLRLQFVQARLQAVSILIYSNALQDNTDKVLYAGFGEELCELIDKEDVHLVEIRAAVLRTLTSMLHFDRNPNVPRAGSRLMKIVHYTGAERQGGTLPLLVRDCIDNLTTHGL---TERYPLILATSLFSLLYHLA-SYEMGGT---ALVKSGMMQSLLCVISWPGVDLEHITFVTRAVRVIDLIT---NIDIARFHQNNGLNVFIDRLEKEIKSCCKALAD 470 HSP 3 Score: 95.5153 bits (236), Expect = 1.516e-18 Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 3
Query: 1455 EIKICLDSEI-DQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
E K L S++ + K + TQR +KSMLN +K+ I D+ ++ M+ L LR I N + P L L + +V+T +V+N PS +S++Q+ +T +L+ L ++ +P R+ L +LP + SAL +N RG+ E DK +K L ++ AMR +R
Sbjct: 598 ERKAELSSQLPSSLAPKKPSCVTQRAALLKSMLNFLKKSIQDHAHFSNMRNIMETSLTQSLRHIIAN-AEYYGPSLFLLATDVVTVYVFNEPSLLSSLQDLGITSVMLKALLQKDVPATREVLGSLPNVFSALCLNERGLFEFLSYDPFDKVLKVLLSPDYLVAMRRRR 765 HSP 4 Score: 92.0485 bits (227), Expect = 1.478e-17 Identity = 90/300 (30.00%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ--QLTGIQLPEN----VDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDL---LSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKP-----------LVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLK 9293
+ EG+DP+FL ALP MR EVI EH R R+RQ Q IQ+ + V+ E +A LP +IQ E+L R E + A NPE V ++F +L +LR+ IL DM++SQIA+L ELA EA+ +R+ D + R R A ++ LS L ++ RW +I Y S Q H + + A PN+ KP L+ D L L+ L + M++ ++++++L H R WI +++I+ K
Sbjct: 3671 VPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIE----QQRQAAQTANPED----------PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRR---DWESRN--------GARMDAHPPSNALSRFQTTLQSLDDTRW-HSSIWYDA-SGNAQPQHHQHTTIVHQHPHAHHAPNMG--KPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRL-----HRVIRNLCHHEPTRDWIISALISIIQK 3936
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Match: HUWE1 (gene:FBgn0030674 transcript:FBtr0339686) HSP 1 Score: 571.622 bits (1472), Expect = 1.134e-162 Identity = 296/572 (51.75%), Postives = 375/572 (65.56%), Query Frame = 3
Query: 10647 KVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKS-------------------------------------DNSNVSDPLS------------HLNTSLVVD---DTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
K L+Q +W+ LS+ L A+++SK+ AV+V Q VE+F L H+ ++ +S D S+ + P S NT LV + T M++ FL+F EKH+ VLN ILR+ + L F+ L+ + ++LDFD+KR+YFQ ++ +L D R EE V V R +FEDS+ L+R EEWK RF I F +EEGQD GGLLREWY+ +S EIFNP YALF VSP D VTY INP+S+ N NHLSYFKFVGR IAKA+ DNKLL CYFTR+FYKHILG +++TD+E +DY+F++GL++L+KND+ST+ + TFS ++ EFG T+ RDL PNG VTEENK EYV+LVCQ KM+G+IRQQL +FLEGFY+IIPK+LISIFNE+ELELLI GLP+ID+ DLK NT Y+KYT S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF LEGMNG KFQI ST+ LP AHTCFN +DLP Y+++DKL CLL AI ECSEG+G
Sbjct: 4575 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQ-SPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL--DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5143 HSP 2 Score: 202.601 bits (514), Expect = 5.652e-51 Identity = 150/503 (29.82%), Postives = 248/503 (49.30%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAAL-GENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVG--HWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLH--LQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVE---DKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIH------CGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEID 1487
MK+D K+ EC +L L +L+ + ++VW YGKCEL W ++LDR D IL E V + L+ D E + ++ +L FT LIEHS R+LY S EH+ L + NMDI+ A+L L Y FSKRSN+ RL +++++ +L +IAE WG +G L +CC+ G + EF+ Q +D ++ H +L + ++ S E+++ + + + + + +M + R+RL F + R + R+QA+SIL Y+N ++ DK L EEL +++ D + I+A LRTLT+++H + S + + TG G P ++R+ I +L + T++ S LYHLA + E+ T LV +G++ S+ +I W + ++I+F TRAVRV D I ++ + +Q + ++ +DRL+ EIK C + D
Sbjct: 1 MKVDTVKLKKGSSELTAECRELIDELTKRRTRAELLEFLDTVHVWIYGKCELYHWIEILDRFDKILEEASRHVNGNEFVLQCDTNFQETDVTLLLHVLNFTTLLIEHSFSRHLYNSIEHLTVLLSSQNMDIVLAVLNLLYMFSKRSNFIPRLPFEKKELLIVKLFNIAERWGDPNYGLSLKDCCI--------GEPKLEFLY--------------QLCID-YVDEHGHAAQLEIPDMMDLCHTASAPEVIKTIAGQISKPSEAIKMRIAHRVRLISGFNNYKLRLQFVQARLQAVSILIYSNALQDNTDKVLYAGFGEELCELIDKEDVHLVEIRAAVLRTLTSMLHFDRNPNVPRAGSRLMKIVHYTGAERQGGTLPLLVRDCIDNLTTHGL---TERYPLILATSLFSLLYHLA-SYEMGGT---ALVKSGMMQSLLCVISWPGVDLEHITFVTRAVRVIDLIT---NIDIARFHQNNGLNVFIDRLEKEIKSCCKALAD 470 HSP 3 Score: 95.5153 bits (236), Expect = 1.503e-18 Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 3
Query: 1455 EIKICLDSEI-DQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
E K L S++ + K + TQR +KSMLN +K+ I D+ ++ M+ L LR I N + P L L + +V+T +V+N PS +S++Q+ +T +L+ L ++ +P R+ L +LP + SAL +N RG+ E DK +K L ++ AMR +R
Sbjct: 598 ERKAELSSQLPSSLAPKKPSCVTQRAALLKSMLNFLKKSIQDHAHFSNMRNIMETSLTQSLRHIIAN-AEYYGPSLFLLATDVVTVYVFNEPSLLSSLQDLGITSVMLKALLQKDVPATREVLGSLPNVFSALCLNERGLFEFLSYDPFDKVLKVLLSPDYLVAMRRRR 765 HSP 4 Score: 92.0485 bits (227), Expect = 1.567e-17 Identity = 90/300 (30.00%), Postives = 136/300 (45.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQ--QLTGIQLPEN----VDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDL---LSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKP-----------LVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLK 9293
+ EG+DP+FL ALP MR EVI EH R R+RQ Q IQ+ + V+ E +A LP +IQ E+L R E + A NPE V ++F +L +LR+ IL DM++SQIA+L ELA EA+ +R+ D + R R A ++ LS L ++ RW +I Y S Q H + + A PN+ KP L+ D L L+ L + M++ ++++++L H R WI +++I+ K
Sbjct: 3665 VPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIE----QQRQAAQTANPED----------PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRR---DWESRN--------GARMDAHPPSNALSRFQTTLQSLDDTRW-HSSIWYDA-SGNAQPQHHQHTTIVHQHPHAHHAPNMG--KPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRL-----HRVIRNLCHHEPTRDWIISALISIIQK 3930
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Match: huwe1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-081104-387]) HSP 1 Score: 536.954 bits (1382), Expect = 4.540e-152 Identity = 274/493 (55.58%), Postives = 340/493 (68.97%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNS 12119
+W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ + P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ E+ K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY FF+GL +LL+NDVST+ + TFS ++ EFG E RDL NG+ ILVTEENK EYV LVCQ KMTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY +S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHT S
Sbjct: 3975 LWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLSHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSNLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRILDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEDMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRVVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYPFFQGLVYLLENDVSTLGYELTFSTEVQEFGVCEVRDLKANGANILVTEENKKEYVHLVCQMKMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKANTEYHKYQSSSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTSLTS 4463 HSP 2 Score: 369.392 bits (947), Expect = 2.807e-101 Identity = 238/717 (33.19%), Postives = 364/717 (50.77%), Query Frame = 3
Query: 12 LYEMKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECC----VTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNM---TGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQI----LLSFQLNG-------------------------------SSTNQKSTIDAIVD----------------RLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
L MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D IL + V + W L D+P N L ++A+L FT LIE+S R+LY S EH+ L + +M ++ ++L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +++Y P SA T+ +EF + SS K+ S+ TL +H++ ESPSE+ME+L YN+ Q+ LF +RL F ++R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ L IKA +LRTLT+I+H + P L N+ TG SYHG P ++R I ++ +PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I + +FQ +G S+ + +D +D + E S + V QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG++ + + ++ K L ++ AMR +R
Sbjct: 2 LVTMKVDRSKLKKTPTEAPADCRTLIEKLKGCSDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILCDAGQTVENMSWLLVCDRPENGQLKALLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASCDMQVVLSVLNLLYVFSKRSNYITRLGSDKRSPLLARLQHLAESWGGKENGFGLAECCRDLPMSKY-PPSATTLHFEFYAEPSSEVKVEKKSSSN----------------TLHYIHIEQLDKISESPSEIMESLTAMYNIPK-DKQILLFTHIRLAHGFSNHKRRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQ-LDIKAASLRTLTSIVHLERT----PKLSNIIDCTGTASYHGFLPVLVRNCIQAMIDPLMEPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHGGLSIFICRLEHEVDLSRKECPFVIKPKIQRPSTATETEDMDTDMDMSEVAMESSPGPSTSSGSRAETDSRAQSNASSTPRAGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLHSFVQCQPFERLFKVLLSPDYLPAMRRRR 686 HSP 3 Score: 68.5514 bits (166), Expect = 2.849e-10 Identity = 78/303 (25.74%), Postives = 134/303 (44.22%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVI-------------IEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLS------VTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFS-ERGQTNRHNNAA-VTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
+ EG+DP+FL ALP+ +RREV+ + L G V E +A LP IQEE+LA R E E + P+ G PL +F+ +L LRR +L DM+ S +A + ++A EA +R+E Q + + L ++ R+ TS L NRG ++Y + +RG T + +A + P + + + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL + S
Sbjct: 2974 LPEGVDPSFLAALPEDIRREVLQNQLGIRPPSRPPVASTLTTTASAVLGGGPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELSQQPT-----QGDQPLDP-------VTFIQTLPSELRRSVLEDMEDSVLAVMPPDIAAEAAALRREQEARQRQLMHERLFGHSSSSALSAILRSPAFTSRLGG-----NRG------VQYTRLAVQRGGTFQMGGSANHSRPSSSNVDSLLRLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHSQTRGWVIRSLLSILQRSS 3248 HSP 4 Score: 65.855 bits (159), Expect = 1.681e-9 Identity = 78/318 (24.53%), Postives = 121/318 (38.05%), Query Frame = 3
Query: 4782 MVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDV-IYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML-------LPSKSK------------DQR-----------------------KFENLSEYEHKIISCVPDPLGDEEVV--ELSDNMLQFLIS--IKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSA--IPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
++T L+K+ ++ A Q + K IT + L+ID YE+ A + + R + Y W W DDR G W S +N+ I+ ++R G+ Y F ++ Q NEE+ RP+ML +P +K +QR + L + S + DP G +V L+++M+ LI + I D L A L +RL + A A KS I L G+T V L R E P L+HT+ ++ V+ GA
Sbjct: 1592 VLTVLIKLLEVVQPCLQAAKEQKDI---QTPKWITPVLLLIDFYEKMAVSSKRRAQMNKYLQPNGNNWRWFDDRSGRWCSYSASNNSTIDSAWRAGETSVRFTA-GRRRYTVQFNTMVQVNEETGNRRPVMLTVQRVPRIPKATKSGSVTDTEREESEQRAKTEETQTDTDSSSVAVEMAPPKEESELKDSTPAAPSVLQDPSGSSGIVVQGLTEDMITILIRACVSMISVPVDPDTLHATLRLCLRLTRTHHYAM-MFAELKSTRMILNLTQSSGFNGFT----PLVTLLFRHIIEDPATLRHTMEKVVRSAVTSGA 1900 HSP 5 Score: 57.7658 bits (138), Expect = 4.552e-7 Identity = 27/73 (36.99%), Postives = 48/73 (65.75%), Query Frame = 3
Query: 2562 VYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 800 IPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVSILGLPNLPIDFPTSAACQAVAGVCKSILTLS 871
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Match: BX664745.2 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:559439]) HSP 1 Score: 372.089 bits (954), Expect = 2.420e-102 Identity = 236/714 (33.05%), Postives = 363/714 (50.84%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECC----VTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNM---TGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQI----LLSFQLNG-------------------------------SSTNQKSTIDAIVD----------------RLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D IL + V + W L D+P N L ++A+L FT LIE+S R+LY S EH+ L + +M ++ ++L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +++Y P SA T+ +EF + SS K+ S+ TL +H++ ESPSE+ME+L YN+ Q+ LF +RL F ++R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + P L N+ TG SYHG P ++R I ++ +PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I + +FQ +G S+ + +D +D + E S + V QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG++ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRSKLKKTPTEAPADCRTLIEKLKGCSDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILCDAGQTVENMSWLLVCDRPENGQLKALLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASCDMQVVLSVLNLLYVFSKRSNYITRLGSDKRSPLLARLQHLAESWGGKENGFGLAECCRDLPMSKY-PPSATTLHFEFYAEPSSEVKVEKKSSSN----------------TLHYIHIEQLDKISESPSEIMESLTAMYNIPK-DKQILLFTHIRLAHGFSNHKRRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLVDIKAASLRTLTSIVHLERT----PKLSNIIDCTGTASYHGFLPVLVRNCIQAMIDPLMEPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHGGLSIFICRLEHEVDLSRKECPFVIKPKIQRPSTATETEDMDTDMDMSEVAMESSPGPSTSSGSRAETDSRAQSNASSTPRAGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLHSFVQCQPFERLFKVLLSPDYLPAMRRRR 683 HSP 2 Score: 66.2402 bits (160), Expect = 1.242e-9 Identity = 85/355 (23.94%), Postives = 135/355 (38.03%), Query Frame = 3
Query: 4770 KNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDV-IYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML-------LPSKSK-----DQRKFEN------------------------------LSEYEHKIISCVPDPLGDEEVV--ELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSA--IPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFE 5687
++ ++T L+K+ ++ A Q + K IT + L+ID YE+ A + + R + Y W W DDR G W S +N+ I+ ++R G+ Y F ++ Q NEE+ RP+ML +P +K D + E+ L + S + DP G +V L+++M+ LI + I D L A L +RL + A A KS I L G+T LVT L R E P L+HT+ ++ V+ GA S+++ Y + P R+ E
Sbjct: 1614 ESSGVLTVLIKLLEVVQPCLQAAKEQKDI---QTPKWITPVLLLIDFYEKMAVSSKRRAQMNKYLQPNGNNWRWFDDRSGRWCSYSASNNSTIDSAWRAGETSVRFT-AGRRRYTVQFNTMVQVNEETGNRRPVMLTVQRVPRIPKATKSGSVTDTEREESEQRAKTEETQTDTDSSSVAVEMAPPKEESELKDSTPAAPSVLQDPSGSSGIVVQGLTEDMITILIRACVSMISVPVDPDTLHATLRLCLRLTRTHHYAM-MFAELKSTRMILNLTQSSGFNGFT-PLVTL---LFRHIIEDPATLRHTMEKVVRSAVTSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPE 1959 HSP 3 Score: 58.151 bits (139), Expect = 3.535e-7 Identity = 27/73 (36.99%), Postives = 48/73 (65.75%), Query Frame = 3
Query: 2562 VYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 797 IPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVSILGLPNLPIDFPTSAACQAVAGVCKSILTLS 868
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Match: huwe1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-081104-387]) HSP 1 Score: 372.089 bits (954), Expect = 2.435e-102 Identity = 236/714 (33.05%), Postives = 363/714 (50.84%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECC----VTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNM---TGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQI----LLSFQLNG-------------------------------SSTNQKSTIDAIVD----------------RLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D IL + V + W L D+P N L ++A+L FT LIE+S R+LY S EH+ L + +M ++ ++L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +++Y P SA T+ +EF + SS K+ S+ TL +H++ ESPSE+ME+L YN+ Q+ LF +RL F ++R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + P L N+ TG SYHG P ++R I ++ +PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I + +FQ +G S+ + +D +D + E S + V QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG++ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRSKLKKTPTEAPADCRTLIEKLKGCSDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILCDAGQTVENMSWLLVCDRPENGQLKALLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASCDMQVVLSVLNLLYVFSKRSNYITRLGSDKRSPLLARLQHLAESWGGKENGFGLAECCRDLPMSKY-PPSATTLHFEFYAEPSSEVKVEKKSSSN----------------TLHYIHIEQLDKISESPSEIMESLTAMYNIPK-DKQILLFTHIRLAHGFSNHKRRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLVDIKAASLRTLTSIVHLERT----PKLSNIIDCTGTASYHGFLPVLVRNCIQAMIDPLMEPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHGGLSIFICRLEHEVDLSRKECPFVIKPKIQRPSTATETEDMDTDMDMSEVAMESSPGPSTSSGSRAETDSRAQSNASSTPRAGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLHSFVQCQPFERLFKVLLSPDYLPAMRRRR 683 HSP 2 Score: 66.2402 bits (160), Expect = 1.242e-9 Identity = 85/355 (23.94%), Postives = 135/355 (38.03%), Query Frame = 3
Query: 4770 KNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDV-IYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML-------LPSKSK-----DQRKFEN------------------------------LSEYEHKIISCVPDPLGDEEVV--ELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSA--IPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFE 5687
++ ++T L+K+ ++ A Q + K IT + L+ID YE+ A + + R + Y W W DDR G W S +N+ I+ ++R G+ Y F ++ Q NEE+ RP+ML +P +K D + E+ L + S + DP G +V L+++M+ LI + I D L A L +RL + A A KS I L G+T LVT L R E P L+HT+ ++ V+ GA S+++ Y + P R+ E
Sbjct: 1621 ESSGVLTVLIKLLEVVQPCLQAAKEQKDI---QTPKWITPVLLLIDFYEKMAVSSKRRAQMNKYLQPNGNNWRWFDDRSGRWCSYSASNNSTIDSAWRAGETSVRFT-AGRRRYTVQFNTMVQVNEETGNRRPVMLTVQRVPRIPKATKSGSVTDTEREESEQRAKTEETQTDTDSSSVAVEMAPPKEESELKDSTPAAPSVLQDPSGSSGIVVQGLTEDMITILIRACVSMISVPVDPDTLHATLRLCLRLTRTHHYAM-MFAELKSTRMILNLTQSSGFNGFT-PLVTL---LFRHIIEDPATLRHTMEKVVRSAVTSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPE 1966 HSP 3 Score: 58.151 bits (139), Expect = 3.536e-7 Identity = 27/73 (36.99%), Postives = 48/73 (65.75%), Query Frame = 3
Query: 2562 VYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 797 IPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVSILGLPNLPIDFPTSAACQAVAGVCKSILTLS 868
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Match: hace1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:572430]) HSP 1 Score: 328.946 bits (842), Expect = 8.393e-94 Identity = 181/459 (39.43%), Postives = 273/459 (59.48%), Query Frame = 3
Query: 10878 SLVVDDTFMNSPAFSENGKHNP-FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLD----------FDIKRRYFQAKVAQLRNDGDSRY----EEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNK-YTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
S V+ +M +G +P F+EFV KH +VL + R + +F LL+ P+L+ F + +F + + D D + E +++ V R LF S + + + E+ KQ ++F EEG G G++REW+ +S+EI NP+YALF S DG T+ N S VN +HL+YF+F G+ + A++ +L+ YFTR+FYKHILGI + Y D+ D ++ + L+++L ND+S + L+ TFS++ FG E L P G+ I VT++NK EYV+LV + +MT AI+ Q+++FL+GF+ IP +LI +F+E ELELL+ G+PEID+ D K NT Y Y P IQWFW+ +E+ QE+R +QFVTG+S+VP GGF+ L G +G KF ++ ++N LP + TC N + LP+Y + D L LL A+ S GY M
Sbjct: 449 SAVIQAFYMCCSCQMPHGMTSPRFIEFVCKHDEVLKCFVTRNPKIIFNHFHF--LLECPELMSRFMHIIKGQPFKDRCEWFYEHLLAGQPDSDMVHRPVNENDILLVHRDSLFRSSCEVVSKSSNEKLKQGIAVRFHGEEGM-GQGVVREWFDILSNEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALYHRQLVNIYFTRSFYKHILGIPVSYQDVSSIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGTMEEVPLKPGGTTIQVTQDNKEEYVQLVTELRMTRAIQPQINAFLQGFHTFIPPSLIQLFDEYELELLLSGMPEIDVMDWKRNTEYTSGYDLQEPVIQWFWEVVENLTQEERVLLLQFVTGSSRVPHGGFAFLMGGSGLQKFTVAAVPYTSNLLPTSSTCINMLKLPEYPSKDVLRDRLLVALHCGSYGYTM 904
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Match: smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:563633]) HSP 1 Score: 321.627 bits (823), Expect = 1.163e-92 Identity = 162/348 (46.55%), Postives = 221/348 (63.51%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRE 12185
++VSR +FE+SY ++ + ++ +R +KF EEG D GG+ REW +SHE+ NP Y LF+ S D + T INP S VN HLSYF FVGR + A+F + FT FYK +LG I D+E D L ++L ND+ T LD TF ++ + +G+ +L PNG I VT++ K EYVRL + I Q + +GF E+IP++L+ F+EKELEL++CGL +ID+ND K NT TP S ++WFW+A+ES+D+E RAR +QFVTG+S+VPL GF L+G GP F I STN+LP AHTCFN ID+P YE++DKL+ LLTAI E
Sbjct: 412 IEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDI-TGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEE 758
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:NCBI gene;Acc:780194]) HSP 1 Score: 335.495 bits (859), Expect = 4.331e-97 Identity = 175/418 (41.87%), Postives = 252/418 (60.29%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ ++K +I R ALD A ++ DF K +YF+ QL + + V+R LFEDS+ ++ A++ ++R I EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ N++ L+ FS+ G+ ++ DL P+GS I VTEENK EY+RLV + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + NT+Y YT S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 365 FVDHNRKTTTYIDPRTGKSALDNGPHIAYVR-----DFKAKVQYFRFWCQQLYMPQHVK-----ITVNRKTLFEDSFQQIMSFNAQDLRRRLWIIIPGEEGLDYGGVAREWFFLLSHEVMNPMYCLFEYAGKDNYCLQINPASYINPDHLRYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVKSHDLKPDGSNIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRNTIYRHYTRTSKQIIWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 770
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:NCBI gene;Acc:780194]) HSP 1 Score: 335.495 bits (859), Expect = 1.295e-96 Identity = 175/418 (41.87%), Postives = 252/418 (60.29%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ ++K +I R ALD A ++ DF K +YF+ QL + + V+R LFEDS+ ++ A++ ++R I EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ N++ L+ FS+ G+ ++ DL P+GS I VTEENK EY+RLV + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + NT+Y YT S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 414 FVDHNRKTTTYIDPRTGKSALDNGPHIAYVR-----DFKAKVQYFRFWCQQLYMPQHVK-----ITVNRKTLFEDSFQQIMSFNAQDLRRRLWIIIPGEEGLDYGGVAREWFFLLSHEVMNPMYCLFEYAGKDNYCLQINPASYINPDHLRYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVKSHDLKPDGSNIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRNTIYRHYTRTSKQIIWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 819
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:NCBI gene;Acc:780194]) HSP 1 Score: 336.265 bits (861), Expect = 1.296e-96 Identity = 175/418 (41.87%), Postives = 252/418 (60.29%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ ++K +I R ALD A ++ DF K +YF+ QL + + V+R LFEDS+ ++ A++ ++R I EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ N++ L+ FS+ G+ ++ DL P+GS I VTEENK EY+RLV + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + NT+Y YT S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 446 FVDHNRKTTTYIDPRTGKSALDNGPHIAYVR-----DFKAKVQYFRFWCQQLYMPQHVK-----ITVNRKTLFEDSFQQIMSFNAQDLRRRLWIIIPGEEGLDYGGVAREWFFLLSHEVMNPMYCLFEYAGKDNYCLQINPASYINPDHLRYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVKSHDLKPDGSNIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRNTIYRHYTRTSKQIIWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 851
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500]) HSP 1 Score: 320.857 bits (821), Expect = 4.558e-96 Identity = 161/350 (46.00%), Postives = 229/350 (65.43%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECS 12191
V+VSR +FE+SY ++ + ++ K+R +KF EEG D GG+ REW + HE+ NP Y LF+ S D + T INP S +N +HLSYF FVGR + A+F + FT FYK +LG I+ +DLE D + + L ++L+ND++++ LD TF ++ + FG+ +L PNG + VTEENK EYVRL + I Q + +GF E+IP++L+ F +KELEL+I GL +ID++D K NT S +QWFWQA+ESFD+E RAR +QFVTG+++VPL GF L+G GP F I L +T++LP AHTCFN ID+P YE+++KL++ LLTA+ E S
Sbjct: 91 VEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETS 439
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500]) HSP 1 Score: 321.242 bits (822), Expect = 9.395e-93 Identity = 161/350 (46.00%), Postives = 229/350 (65.43%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECS 12191
V+VSR +FE+SY ++ + ++ K+R +KF EEG D GG+ REW + HE+ NP Y LF+ S D + T INP S +N +HLSYF FVGR + A+F + FT FYK +LG I+ +DLE D + + L ++L+ND++++ LD TF ++ + FG+ +L PNG + VTEENK EYVRL + I Q + +GF E+IP++L+ F +KELEL+I GL +ID++D K NT S +QWFWQA+ESFD+E RAR +QFVTG+++VPL GF L+G GP F I L +T++LP AHTCFN ID+P YE+++KL++ LLTA+ E S
Sbjct: 375 VEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETS 723
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Match: Huwe1 (HECT, UBA and WWE domain containing 1 [Source:MGI Symbol;Acc:MGI:1926884]) HSP 1 Score: 585.489 bits (1508), Expect = 8.326e-167 Identity = 295/522 (56.51%), Postives = 365/522 (69.92%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3860 LWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4377 HSP 2 Score: 385.185 bits (988), Expect = 5.559e-106 Identity = 236/677 (34.86%), Postives = 363/677 (53.62%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 73.9442 bits (180), Expect = 6.744e-12 Identity = 85/323 (26.32%), Postives = 143/323 (44.27%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPE--------------------NVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNIL-------LKISLPKENT 9317
+ EG+DP+FL ALP +RREV+ Q GI+ P V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL L I PK +T
Sbjct: 2965 LPEGVDPSFLAALPDDIRREVL----------QNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLST 3248 HSP 4 Score: 68.5514 bits (166), Expect = 2.920e-10 Identity = 78/303 (25.74%), Postives = 126/303 (41.58%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP-----SKSKDQRKFENL------SEYEHKIISCVPDPL------------------GDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SKS + ++ E ++ +HK PL G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 2.824e-7 Identity = 31/93 (33.33%), Postives = 56/93 (60.22%), Query Frame = 3
Query: 2508 GKDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 744 AQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Match: Huwe1 (HECT, UBA and WWE domain containing 1 [Source:MGI Symbol;Acc:MGI:1926884]) HSP 1 Score: 585.489 bits (1508), Expect = 8.681e-167 Identity = 295/522 (56.51%), Postives = 365/522 (69.92%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3859 LWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4376 HSP 2 Score: 385.185 bits (988), Expect = 5.605e-106 Identity = 236/677 (34.86%), Postives = 363/677 (53.62%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 73.9442 bits (180), Expect = 6.360e-12 Identity = 85/323 (26.32%), Postives = 143/323 (44.27%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPE--------------------NVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNIL-------LKISLPKENT 9317
+ EG+DP+FL ALP +RREV+ Q GI+ P V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL L I PK +T
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVL----------QNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLST 3247 HSP 4 Score: 68.5514 bits (166), Expect = 2.800e-10 Identity = 78/303 (25.74%), Postives = 126/303 (41.58%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP-----SKSKDQRKFENL------SEYEHKIISCVPDPL------------------GDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SKS + ++ E ++ +HK PL G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 2.823e-7 Identity = 31/93 (33.33%), Postives = 56/93 (60.22%), Query Frame = 3
Query: 2508 GKDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 744 AQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Match: Itch (itchy, E3 ubiquitin protein ligase [Source:MGI Symbol;Acc:MGI:1202301]) HSP 1 Score: 334.724 bits (857), Expect = 3.919e-96 Identity = 171/418 (40.91%), Postives = 254/418 (60.77%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ +++ +I R ALD A ++ DF K +YF+ QL + + + V+R LFEDS+ ++ + ++ ++R + F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ +N++ L+ FS+ G+ ++ DL PNG ILVTEENK EY+R+V + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + + +Y YT S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 457 FVDHNRRATTYIDPRTGKSALDNGPQIAYVR-----DFKAKVQYFRFWCQQL-----AMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 862
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Match: Itch (itchy, E3 ubiquitin protein ligase [Source:MGI Symbol;Acc:MGI:1202301]) HSP 1 Score: 334.724 bits (857), Expect = 3.919e-96 Identity = 171/418 (40.91%), Postives = 254/418 (60.77%), Query Frame = 3
Query: 10953 FVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
FV+ +++ +I R ALD A ++ DF K +YF+ QL + + + V+R LFEDS+ ++ + ++ ++R + F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ +N++ L+ FS+ G+ ++ DL PNG ILVTEENK EY+R+V + +++ + +Q +F EGF EI+P+ + F+ KELE+L+CG+ EIDLND + + +Y YT S QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 457 FVDHNRRATTYIDPRTGKSALDNGPQIAYVR-----DFKAKVQYFRFWCQQL-----AMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 862
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Match: Nedd4l (neural precursor cell expressed, developmentally down-regulated gene 4-like [Source:MGI Symbol;Acc:MGI:1933754]) HSP 1 Score: 319.316 bits (817), Expect = 1.427e-94 Identity = 175/388 (45.10%), Postives = 247/388 (63.66%), Query Frame = 3
Query: 11049 PKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHR-KTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYV-NNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGY 12200
P +F K YF+ K+ + D +R+E +++ R +FE+SY R+ K + K R I+F +E+G D GG+ REW+ +S E+FNP Y LF+ S D T INP S + N +HLSYF F+GR A+F KLL +F R FYK +LG I D+E D +++ L+++L+ND + LD F I FGQT DL PNGS+I+VT ENK EY+ LV Q + +++Q+++FLEGF E++P +LI IF+E ELELL+CGL ++D+ND + +++Y N Y P P IQWFW+A+ D E R R +QFVTGTS+VP+ GF++L G NGP F I S LP AHTCFN +DLP YE F+ L + LL A+ E ++G+
Sbjct: 144 PYSREFKQKYDYFRKKLKK-PADIPNRFE---MKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTE--LDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWG-SPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAV-ENAQGF 523
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Match: sp|Q7Z6Z7|HUWE1_HUMAN (E3 ubiquitin-protein ligase HUWE1 OS=Homo sapiens OX=9606 GN=HUWE1 PE=1 SV=3) HSP 1 Score: 593.578 bits (1529), Expect = 2.336e-168 Identity = 297/532 (55.83%), Postives = 370/532 (69.55%), Query Frame = 3
Query: 10662 ILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+L++ S +W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3846 LLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4373 HSP 2 Score: 385.956 bits (990), Expect = 2.310e-105 Identity = 236/677 (34.86%), Postives = 364/677 (53.77%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 77.0258 bits (188), Expect = 5.483e-12 Identity = 81/300 (27.00%), Postives = 139/300 (46.33%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEH-------RRA---NRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
+ EG+DP+FL ALP +RREV+ R A N + G V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL + S
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSS 3234 HSP 4 Score: 67.3958 bits (163), Expect = 4.639e-9 Identity = 78/303 (25.74%), Postives = 123/303 (40.59%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP--------------------SKSKDQRKFENL---------SEYEHKIISCVPDPLGDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SK D ++ EN S K S +G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 2.315e-6 Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 3
Query: 2511 KDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 745 QQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Match: sp|Q7TMY8|HUWE1_MOUSE (E3 ubiquitin-protein ligase HUWE1 OS=Mus musculus OX=10090 GN=Huwe1 PE=1 SV=5) HSP 1 Score: 585.489 bits (1508), Expect = 6.072e-166 Identity = 295/522 (56.51%), Postives = 365/522 (69.92%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ EE K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 3859 LWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRVLDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 4376 HSP 2 Score: 385.185 bits (988), Expect = 3.921e-105 Identity = 236/677 (34.86%), Postives = 363/677 (53.62%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYM---PYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKIC-------LDSEIDQ--MTKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKR 1958
MK+DR K+ +C L +L + L+ E+++I W+ GKCEL W D+LDR D ILA+ V + W L D+P E L ++A+L FT LIE+S R+LY S EH+ L +S+M ++ A+L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC M P SA T+ +EF D G E +K + TL +H++ ESPSE+ME+L + Y++ QM LF +RL F +R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + ++ + TG SYHG P ++R I ++ PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ +C + +I + T++ + T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG+ + + ++ K L ++ AMR +R
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYAD------PGAE----------VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPK-DKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSII-DCTGTASYHGFLPVLVRNCIQAMIDPSMDPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRR 647 HSP 3 Score: 73.9442 bits (180), Expect = 4.448e-11 Identity = 85/323 (26.32%), Postives = 143/323 (44.27%), Query Frame = 3
Query: 8454 IAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPE--------------------NVDAEIMAQLPAHIQEEILAHYRRENSTSETA-NAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLL------SVTGRRRARQLTSGLSDLLSQMNRG-RWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNIL-------LKISLPKENT 9317
+ EG+DP+FL ALP +RREV+ Q GI+ P V E +A LP IQEE+LA R E E A NA SS T + +F+ +L LRR +L DM+ S +A + ++A EA+ +R+E Q + + L +++ R+ TS LS NRG ++ + ++ + G ++ HN + ++ T + L+ + L+ L+ LL K+ ++ ++++L++L H Q R W+ ++++IL L I PK +T
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVL----------QNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNA--------------SSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSG-----NRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLL-----RLRGRLLLDHEALSCLLVLLFVDEP-----KLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLST 3247 HSP 4 Score: 68.5514 bits (166), Expect = 1.958e-9 Identity = 78/303 (25.74%), Postives = 126/303 (41.58%), Query Frame = 3
Query: 4794 LVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVI-YEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LP-----SKSKDQRKFENL------SEYEHKIISCVPDPL------------------GDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLC--DKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGA 5588
L+K+ ++ A Q E K IT + L+ID YE+TA + + R + Y W W DDR G W S +N+ I+ ++++G+ Y F ++ Q NEE+ RP+ML +P SKS + ++ E ++ +HK PL G+ + L+++M+ LI + + D L A L +RL KY++ L + + I L G+T LVT LLR E P L+HT+ ++ + GA
Sbjct: 1557 LIKLLEVVQPCLQAAKEQKEV---QTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFT-AGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTR-MILNLTQSSGFNGFT-PLVTL---LLRHIIEDPCTLRHTMEKVVRSAATSGA 1850 HSP 5 Score: 58.5362 bits (140), Expect = 1.975e-6 Identity = 31/93 (33.33%), Postives = 56/93 (60.22%), Query Frame = 3
Query: 2508 GKDNKTEEMNQFSCDDD--AVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ N+TE Q ++ + LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 744 AQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLS 835
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Match: sp|P51593|HUWE1_RAT (E3 ubiquitin-protein ligase HUWE1 (Fragment) OS=Rattus norvegicus OX=10116 GN=Huwe1 PE=1 SV=2) HSP 1 Score: 474.937 bits (1221), Expect = 1.224e-150 Identity = 224/321 (69.78%), Postives = 264/321 (82.24%), Query Frame = 3
Query: 11247 EEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY F++GL +LL+NDVST+ D TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ +MTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+F+KL LL AI+ECSEG+G+
Sbjct: 1 EEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL 321
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Match: sp|Q8GY23|UPL1_ARATH (E3 ubiquitin-protein ligase UPL1 OS=Arabidopsis thaliana OX=3702 GN=UPL1 PE=1 SV=3) HSP 1 Score: 416.001 bits (1068), Expect = 1.417e-114 Identity = 209/428 (48.83%), Postives = 286/428 (66.82%), Query Frame = 3
Query: 10944 FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEEL-VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISE-----FGQTETRD--LIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
F +F EKH+++LN +R+ + L + + S +LK P+L+DFD K+ YF++++ R+ D L + V RA + EDSY +L ++ ++ K R ++F EEG D GGL REWY +S IF+ LF + T+ NP S HLSYFKFVGR +AKA+FD +LL YFTR+FYKHILG+ + Y D+E D +++ L++LL+NDVS + LD TFS+ E + +TE D L P G I VTEE K EYV LV +T AIR Q+++FLEGF E+IP+ L+SIFN+KELELLI GLPEID +DLK NT Y YT SP I WFW+ +++F +ED ARF+QFVTGTSKVPL GF L+G++GP + QI A + LP+AHTCFN +DLP+Y++ ++L + LL AI E SEG+G
Sbjct: 3258 FSKFAEKHRRLLNSFIRQNPS--LLEKSLSMMLKAPRLIDFDNKKAYFRSRI---RHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGFG 3679 HSP 2 Score: 83.5741 bits (205), Expect = 6.213e-14 Identity = 120/636 (18.87%), Postives = 251/636 (39.47%), Query Frame = 3
Query: 117 DLINEIKKINVWHYGKCELGLWADVLDRLDNILAE--GVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWE-HILALFGTSNMDILHALLGLFYNFSKR--SNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCV---TEYMPYSAG-TMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSW-----DIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQ-MTKKSVALNTQRVGFIKSMLN-----------LIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAM 1946
+LI+E W + K + W D+ + D + V K H ++ ++ +L ++E+ ++ Y S+E H+ L +++ D++ A L F KR Y R S N K+ S+A+ WGGK+ G GL C + + G T+ +EF S S + V P ++ + ES EL+ L+ +N+ S + AL R+R F R+ R+ A +L + + +S+ + V ELV ++ D I+ L +L A+ + + P + + G +M++ I +V + ++ A L L +S+ + GL+ ++ +I +P ++ + AV + + + + +D + RLK E+ D ++ + S +T+++ + +++++ L + L + + LP+ L +IF F + + V+++ ++ P+ +A+ LT L ++++ + + + + +P L AL +N G+ +K+ + ++K T ++ A+
Sbjct: 28 ELIHEPLACFRWEFDKGDFHHWVDLFNYFDTFFEKHVQVRKDLHIEENFEESDPPFPKDAVLQVLRVIRVVLENCTNKHFYSSYEQHLSLLLASTDADVVEACLQTLAAFLKRQIGKYSIRDASLNSKLF-----SLAQGWGGKEEGLGLTSCAAENSCDQVSLQLGRTLHFEFYPSDESPSEL------PGGLQVIHVP----------DVSICAESDLELLNKLVIDHNVP-PSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDTENVVSFFNGEPEFVNELVTLVSYEDTVPEKIRILCLLSLVAL---SQDRTRQPTVLTAVTSGGHRGLLSGLMQKAIDSVV---------CITSKWSLAFAEALLSLVTVLVSSSSGCSAMREAGLIPTLVPLI--KDTDPQHLHLVSAAVHILEAFMDYSNPAAALFRDLGGLDDTIFRLKLEVSRTEDDVKEKNCSSDSNGPDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYGSEESLLPECLCIIF-RRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSTFLDAISDEVI-CSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFTSPSYLRAL 625 HSP 3 Score: 63.929 bits (154), Expect = 4.545e-8 Identity = 69/259 (26.64%), Postives = 109/259 (42.08%), Query Frame = 3
Query: 8415 NDEDIDNLSMEQLIAE--GIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLP-------ENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLS-DLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALL 9161
NDE ++ S++ E IDPTFLEALP+ +R EV+ QQ +Q P +++D E +A LP IQ E+LA R + ++ + + ++S + +L LR E+L ++ +A L S L EA+ +R D M QA R +GL + L+ M+RG V +R ++ + V + + PLV+ D L +LI LL
Sbjct: 2590 NDEQVEEGSLDGRAPEVNAIDPTFLEALPEDLRAEVL--------ASQQAQSVQPPTYEPPPVDDIDPEFLAALPPDIQTEVLAQQRAQRMVQQSQG----------------QAVDMDNASIIATLPADLREEVLLTSSEAVLAALPSPLLAEAQMLR----DRAMSHYQARSSVFGSSHRLNNRRNGLGYNRLTGMDRGVG-------VTIGQRAVSSSADGLKVKEIEGD-----------PLVNADALKSLIRLL 2802
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Match: sp|Q8H0T4|UPL2_ARATH (E3 ubiquitin-protein ligase UPL2 OS=Arabidopsis thaliana OX=3702 GN=UPL2 PE=1 SV=3) HSP 1 Score: 416.001 bits (1068), Expect = 1.518e-114 Identity = 209/428 (48.83%), Postives = 288/428 (67.29%), Query Frame = 3
Query: 10944 FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEEL-VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISE-----FGQTETRD--LIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
F +FVEKH+++LN +R+ + L + +FS +LK P+L+DFD K+ YF++++ R+ D L + V RA + EDSY +L ++ ++ K R ++F EEG D GGL REWY +S IF+ LF + T+ NP S HLSYFKFVGR +AKA+FD +LL YFTR+FYKHILG+ + Y D+E D +++ L++LL+NDVS + LD TFS+ E + +TE D L P G I VTEE K EYV LV +T AIR Q+++FLEG E+IP+ L+SIFN+KELELLI GLPEID +DLK NT Y YT SP I+WFW+ +++F +ED ARF+QFVTGTSKVPL GF L+G++GP + QI A S LP+AHTCFN +DLP+Y++ +++ + LL AI E +EG+G
Sbjct: 3235 FSKFVEKHRRLLNSFVRQNPS--LLEKSFSMMLKAPRLIDFDNKKAYFRSRI---RHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGFG 3656 HSP 2 Score: 74.3294 bits (181), Expect = 3.240e-11 Identity = 123/640 (19.22%), Postives = 253/640 (39.53%), Query Frame = 3
Query: 117 DLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLK--LDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWE-HILALFGTSNMDILHALLGLFYNFSKR--SNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAEC-----CVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSW-----DIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIK---------ICL------DSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAM 1946
+LI E W + K + W D+ D + V ++ D+ ++ +L ++E+ + Y S+E H+ L +++ D++ A L F KR Y R S N K+ S+A+ WGGK+ G GL C C ++ T+ +EF S S + V P ++ ++ ES EL+ L+ +N+ S + AL RLR F R+ R+ A +L + + +S+ + V ELV ++ D I+ L++L A+ + + P + + G +M++ I ++ + S + A+LS + L + S+ + GL+ ++ +I +P ++ + AV + + + + +D + RLK E+ +C + + +Q+ ++ R +K++L I L + L + + LP+ L +IF F + + V+++ ++ P+ +A+ LT L ++++ + + + + +P L AL +N G+ +K+ + ++K + ++ A+
Sbjct: 28 ELIQEPLASFRWEFDKGDFHHWVDLFYHFDTFFEKHVKVRKDLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENCTNKQFYTSYEQHLSLLLASTDADVVEACLQTLAAFLKRPTGKYSIRDASLNLKLF-----SLAQGWGGKEEGLGLTSCATEHSCDQLFLQLGC-TLLFEFYASDESPSEL------PGGLQVIHVP----------DVSMRSESDLELLNKLVIDHNVP-PSLRFALLTRLRFARAFSSLATRQQYTCIRLYAFIVLVQASGDTENVVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVAL---SQDRTRQPTVLTAVTSGGHRGLLSGLMQKAIDSVICN-----TSKWSLAFAEALLSLVTVLVSS----SSGCSAMREAGLIPTLVPLI--KDTDPQHLHLVSTAVHILEVFMDYSNPAAALFRDLGGLDDTIFRLKQEVSRTEDDVKEIVCCSGSNGPEDDTEQLPYSEALISYHRRLLLKALLRAIS-LGTYAPGNTNLYGSEESLLPECLCIIF-RRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSAFLDAISDEVI-CSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFSSPSYLKAL 627 HSP 3 Score: 63.5438 bits (153), Expect = 5.462e-8 Identity = 71/258 (27.52%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 8415 NDEDIDNLSMEQLIAE--GIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLP-------ENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALL 9161
NDE +D S++ E IDPTFLEALP+ +R EV+ QQ +Q P +++D E +A LP IQ E+LA R + ++ P + ++S + +L LR E+L ++ +A L L EA+ +R D MR QA R +GL L+ M RG V +R ++ + V + + PLV+ D L +LI LL
Sbjct: 2574 NDEQVDEGSLDGRAPEVNAIDPTFLEALPEDLRAEVL--------ASQQAQSVQPPTYEPPSVDDIDPEFLAALPPEIQREVLAQQRAQRMLQQSQGQP----------------VDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLAEAQMLR----DRAMRHYQARSRVFGSSHRLNNRRNGLGYRLTGMERGVG-------VTIGQRDVSSSADGLKVKEIEGD-----------PLVNADALKSLIRLL 2785
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Match: A0A183SSY5 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1) HSP 1 Score: 821.617 bits (2121), Expect = 0.000e+0 Identity = 565/1564 (36.13%), Postives = 814/1564 (52.05%), Query Frame = 3
Query: 7914 ISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTE----ASVDEKEVVDSKTNMVKTANSNPDNVDNERN------SITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNE---SVIGEEMV--------------TPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSS-TGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTD---PVTEAGEWPNLSAY-KPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS---LPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFP----TSQSKKV------PTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEE----------YDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPVAAEWSIATNDGDNHESSTPTAS----TGNETPLSITKEKSETKAVNVSDPHLNRVNVKTISHL--------VNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKV------DNSI--------LTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDC----SEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFM-------NSPAFSENGKH-------------------------------------NPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRN-DGD-SRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMST-NHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
S++TNFR I R LYG+E+ D+++L+R Y+ H++ + E + + V ++ + T + DN + S DQ +G S ++ + EPN+ SV E + P+ SETM P EQP ++ + + + + D ++ ++AEG+DP+FL+ALP+ +RRE++ +HRR ++R QL+ +QLPE+V AE ++ LP +IQEE+L R+E ++ N ++ T +++FL SL PS+RRE+LADM+ QI L +L EAR +R+E RA+Q S+ G R R T+ LS GRWD R Y + R+ A V P +G S + L+ +GLT ++ LL+ SS P + + ++L++L HP IR WI CTI+++L ++S LP + T ++ S S S L P+ ++S+ F FFH AL Q+ P++ + + S S +H V L+D+++ ++ +FFP SQ ++ P++ L + + E+ H + DYF+ L L+R P++ + EKLLS + +++ + K +P S T N E+ P ++ T +E S + T + V R+ K +S L + +C +++T T++ R S RL+I +++ N T ++ L + ++ L V++E PS + P T S S L+S P NA S R + + ++S+P ++L++ S++P T + + L + +L+ + D S + S +W LSQ LD ++ + + + V++ Q LVES L HS + S VS +S + L + SPAF N H +P E H+ LNHILR + L +S+F A L YP+ LDFDIKRR+F+ ++ + N DG SR+E+E V VSR R+FEDSY RLHRK+A EWK +F I+F NEEGQD GGLLREWYL MS EIFNPNY LF+VSP D VTYTINPAS++N+NHLSYFKFVGRFI KAI+DNKLL CYFTR+FYKH+LG+ +R+ DLE EDY F++GLEFLLK+DVS + D TFS +I+EFG+ ETRDLI +G I+VTE NK+EYVRLVCQE+MTGAIRQQL++FLEGFYEIIPK+LISIFNE+ELELLI GLP ID+ DLK NT Y+KY P SPQ++WFW+ALESFDQEDRARF+QF+TGTSKVPLGGF+ LEGM+GPTKFQIS A +S+ NHLP+AHTCFN+++LP YE++++L + LLTAIRECSEGYGM
Sbjct: 2780 FSILTNFRHLIDVCRILYGQEVMDMILLSR---YEVGVHAIRRRGDEFRERLAAHQASVNNAAASAETTTEATADNSSAAAHVPLLAPSTEDQAFVGAAASDDRYIRASVQSEWAPPHEPNDLASSVRPEASMEHQSASQSEREVAEPPSSSETMSVPSNP------EQPTAGDAQSNVPTPSAAPPLIVTEEIHVPVTDEETIASMVAEGMDPSFLDALPEDVRRELLADHRRTRQLRSQLSSMQLPEHVSAEFLSSLPPNIQEEVLTQVRQETQRQTGSDETQGQNTARAGEAANGAAATPENNAAFLASLPPSVRREVLADMEDFQIDLLPPDLQQEARTLRREHEAQFARAVQGR--SLPGLFRPRLDTTYFWQTLSGF--GRWDSRAGSY------NRLIRNGGAPVLGSRGPSAFSGLQSFFSGRNRHLIDHEGLTCILTLLVVSSACPVQRRHTVPALKRVLRNLASHPGIRNWIICTILSLLKRLSEKPLPPDLTVGGSRL----SPSMQSALRGPM----------ANSLFAAGFEAALGCWVRFFHPLPAFNALPPQL--PAVDESAPGPSTSEPVSTTDVVYIVHPQAVNLVCSTLLDAIADLARNCPPQFFPLAPHVSQRQESISDTTQPSDGSHDLPALPNANTTLSENSATAKHKRNSSASVDVDTDVCCLDYFSQLASLLRHPVISCRPPLQEKLLSILVGVVREFCKRSRALHQP-----STTTAPLINRENVEPASAMAEGTTSEASSSQYAGGAMTSSSAVGGDAPERIPKKPVSDLMLCPQHAVIECLCELVVAAKT---TEQARSLSVRLVILIAQANAKTNAQMLHYLCKAASDLSAIISRQLKAVINEV-----------DSLPPSNKASLPSSSKTTIPLSASMTRLSSVSDPEVSNA-GDPSDRFLAPTLVASLPSSSSVPPATASELELSSLQPLTTSRSEQSRLCCVLHLILHMLSCGSEDTSTNDAHTKVTMESINGLSQLWEHLSQTLDRLETTNDANTVLLLQPLVESLCLAHSTVLRENGSVRRLSRVSTIGVVSSGISFIPNLLPPEADLYELPPITNTSPAFGRNSTHVDSEPLSPASTIDLNRPMSPPLSTLNNATDAVSETASSPIAHLAETHRASLNHILRNFSG-NLSESSFVAFLSYPRALDFDIKRRFFRQRLQAIANRDGSVSRFEDEPVVVSRGRIFEDSYARLHRKSAAEWKHKFVIRFQNEEGQDAGGLLREWYLLMSREIFNPNYCLFRVSPADRVTYTINPASFINSNHLSYFKFVGRFIGKAIYDNKLLECYFTRSFYKHMLGVRVRFVDLESEDYDFYKGLEFLLKHDVSELGYDVTFSTEINEFGKNETRDLIEDGRNIIVTEANKNEYVRLVCQERMTGAIRQQLNAFLEGFYEIIPKSLISIFNEQELELLISGLPNIDIEDLKANTTYSKYQPNSPQVRWFWRALESFDQEDRARFLQFITGTSKVPLGGFANLEGMHGPTKFQISRAVVSSINHLPSAHTCFNTLELPPYESYEQLRERLLTAIRECSEGYGM 4287 HSP 2 Score: 743.421 bits (1918), Expect = 0.000e+0 Identity = 488/1442 (33.84%), Postives = 748/1442 (51.87%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPK---CHRR------KLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILC-----------------------YTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQG-----NLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSE--IDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPS-GVYSSDSNQAADLLAVMTAVEEADREV-----------EQIMEMSGDEDDGVAD----------------------------NEMTQAID------AEEDRTPSDGCGKDNKTEEMNQFSCDDDAV----YLMDYICNVCKFMERFM-SDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLR---------QDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKR----LCINERGTCADNEHNEER------CFLTKKSS---LSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSXXXXXXXXXXXGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSW-PEDPHSELPVEKACQKILSDIKFL---HPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEP 3998
MKIDRKKI NRCG+DQ EC LA L+ L +++ I E+++I VW+YGKCELGLWAD+LDRLD IL T+VG WT+K+D P NE L+ ++ +LEFTGHLIEHSIYR LYGSW HIL LFG+SNMDIL A+LGL YNFSKRSNYFSRLD+ N+K++L+RL SIAE WGG + F LA CC + P AG + +E+ DV++ + K+D + P+ C + + +L ESPS++ME L++K+ + ++MALFAR+RL VYF PE+R I R+QA+S LC YT E+ D+ L +V+ELVDVL+LPD +F+ IKACALRT+TAI ++ + TGM+S+HG PT +R+WI LV +V Q YT A+LSFLYHLA E + G L +G+LDSM ++I WH D +S+ TRAVRVTDQIL++ ++TN+++ ++ +VDRL +E+ + L ++ +D +++ LNTQR G +KS+LNL+KRL LD +W++ ++ +M+G LP IL I+ N F P L LF+ME +TN++Y +PS +S MQE +T IL LT QPLP NRDFLV LP++L+ L++N RG ++ I++KY++TL +++ M+ +R+R+ + S + + T+ QMSN++ EL R++ + KP +++S+ + F+ V +G Q AP+L +P T+ +E+ + ST G + V+SS++ + + AV +A E ++ +SG ED+G A+ T ID +E T +DG D K +++ S DA L +YI N+CKF+ER + S Q + +Q ++ GL+ + D+LT+P LPYDFP S AC S+ KIFE L+S+ + ND + + + + D L K+ + L + S F D A E GV LH LV +S+++ + +I + L R ++IL +L L ++WEA++L+ L ++ + + L ++ T A+ + + + F+ S L C Q+ +++ + +L + N + EL TLT+FC P S R +P K ++L ++A+ L G W P D +L++ FL + ++Y H +L +K P + F AF M NG++ F N+L +L + ++ +LE + V R++ I + E ++ + F+ + Y + L+P
Sbjct: 2 MKIDRKKIGNRCGYDQNECMLLAKHLSTLSDDEFIQELRRIKVWNYGKCELGLWADILDRLDGILENAATRVGAWTIKVDLPENEKLVEDVVTVLEFTGHLIEHSIYRCLYGSWNHILTLFGSSNMDILLAVLGLTYNFSKRSNYFSRLDAANKKMILDRLISIAETWGGSESHFDLASCCQPNHFPPMAGKIYFEYQPDVANSLNHEKKDEVAGISRSVLLPRLVVCPNTMSLSGPDVIIEDLKGCRESPSQIMEALLKKHPVP-LPNRMALFARIRLAVYFSDPEKRLKCIRARLQAISTLCMLCRPQFPSLFQLFTYVMLFFSCYTFEINDRLLYPSLVDELVDVLQLPDGDFMEIKACALRTMTAIFSSHRVNVNLMSILESTGMSSFHGALPTRIRKWIQGLVDGSCDAAGGSVNQQYTIALLSFLYHLASFEENIGSGSGQNHATTLSTSGILDSMLQLIAWHVPRNDCLSYVTRAVRVTDQILMN-----AATNRQTVVNRLVDRLGYEVDLVLSNQGKLDATGGQALLLNTQRSGLMKSILNLLKRLCLDTDWNDAVRTSMEGNLPQILCSIYKNSVFHFTPHLALFAMETVTNYIYTYPSRISNMQENGITCGILSALTTQPLPQNRDFLVHLPSILNTLALNTRGRAALRSCGILNKYLETLVSPDYLSTMKARRVRDFHSQISYSLAPSAGQVFSTNLTASQMSNSIQELLRSHNEL-KPAVFQSLVECFRKVVRLGCSQSAPIL--------------------PSPETLGSSELESEGSCRSFNQSYSTHGRNLNVFSSENRPDSRMTVTSAAVIDATNPTANRTPGGVLLDEDLVMLSGSEDNGSAEDDDENDDMPDVGLTASRTAVSSSTIFRTTARTAHIDTTDSSVSESHSTTTDGDTPDQKPSALSEASISSDAPESFQNLPEYILNLCKFVERLLPSMQHVTEGMCRQFVNQGGLQVLCDLLTMPGLPYDFPVSAACASLCKIFEQLVSYLSLND------LLKPVLQALQDGLSKVS-----NFLASETTEYSLLFTDCSA---------NETGV--LHGLVCISSILCVLVQIINHLKPEHRCGFASLWAQRNILGQL-------GELYHKVSWEAVILLKALCDENKDLTQPGSSTSYPLPVDANETSANTQTAQSKPPFSMPAFVEALSVYRILKPCLQVHQPT-KTKSLAHYILHVCSYFINSVTELCTTLTRFCVASP--SQPQRRLLMSSFDQVPLSA-KTSVLTQVAQ-------------LAIGALCWRPPD------------AVLNNSDFLLFVYAVRYSSVRLAHLLLFDAS-SKSPQILMFNAFYMLNGIQSLFSIFNDLASFNSSTDFL----SGALSTVLEEWFACVYRLSNIVAVKAEVKKMSETDAV-HFNTNKYANYVNRLSLKP 1352 HSP 3 Score: 94.3597 bits (233), Expect = 8.429e-15 Identity = 117/495 (23.64%), Postives = 207/495 (41.82%), Query Frame = 3
Query: 4518 DIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVP-----------HQTETFKTS----VLKHITQLWL----IIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSK----SKDQRKFEN----LSEYEHKIISC-VPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQ--QFETPQLLQ-----HTISDILDKNVSKGAC----VLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQV-----LKTYSISKSPSLTSKATQPLM----ELEKKQIAIVRFLVES 5858
+I+ +I +++L + Q + +E ++A + S L L+ S + D+ GLH++ LL+ + RF + ++ L + + + P + + +S L+ L++ I+DQYER A+R+RK + Y+ C +W W D+R +WHP + + ++ NG +A + N Y F ++TQ N S RP++L+P S D + +++YE II+ PD L + L +L+ + + +CL + LL+RL S A + L + H ++ Q FV ++RQ QF + L HT+ IL ++S +LHK +DLFY + PL+ + E + LM + L EK + K++SI L + A ++ EL +Q IV F++E+
Sbjct: 1532 EILHQISELMLCSKQETQCIENLVAAVTTNLFAS-------LGVLLPTPSSP------VTDTGVGLHLIALLFTRCRFVCAQMVHKYSLILVLTALITDDVDTLRSHPLPVLLNLGESSAKPSAYMSSQSLVALEGDEDLFMLAAIILDQYERAVAAIRLRKKSVELYKDCHSWSWFDERANLWHPQAPDLFQAADAAFHNGATRAQWSID-RNRYAIEFPTMTQVNLSSMHRRPVLLMPCSLSPTSSDSPRPTGSTTQVTDYEGMIINMEPPDGLSADLRRSLCLALLRRF--DRSTEKPLSAECLNSFLRLLLRLT-ASSFEDAETVANADGLRVLFALSHPFEFSSQYSAFVGHIIRQASQFSSEFLFAYVSNLHTL--ILFDDLSTVTVTMQQILHKFAEGVCSSCRDLFYLLSLCAPLVAKAEELAIRLMKETFSLVVD-EKSIEDGVPPKSFSIVN--PLETPANDAIIDVSPELNNRQKHIVVFIIEA 2004
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Match: A0A4Z2CZ75 (E3 ubiquitin-protein ligase HUWE1 (Fragment) OS=Schistosoma japonicum OX=6182 GN=EWB00_006247 PE=4 SV=1) HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0 Identity = 446/1113 (40.07%), Postives = 649/1113 (58.31%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVS-SLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNE---VNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLG----------NGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDS-----QEIASTAGPSGVYSSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDR--TPSDG----------------CGKDNKTEEMNQFSCDDDA---------------VYLMDYICNVCKFMERFMS-DQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQ--LYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAE-LKSGIID-YARLLPLLNWEAMVLMNLLFEDEEACLEDL 3188
MKIDRKK NRCG+DQ EC LA L+ ++ LI+E+K+INVW+YGKCELGLWADVLDRLD IL VTKVG W L+LD PGN L+ ++ ILEFTGHLIEHSIYRYLYGSW HILALFG+SN+D+L A+LGL YNFSKRSNYF RLD +N++++L+RL SIAE WGG ++GFGLA CC ++ P +AG + +E+ + S SSA + N S K + L+ LH + ++PSE+ME L+ + + S QMALF+R+RL YF P QR I R+QALSIL YT EV+++ L +++ELV+VL LPD +++GIKACALRT+TAI++ +L W L TG++SYHG P + R+WI LV + + A + Q +TTA+LSFLYHLAC E +T L +G+LDSM ++I WHT DY+S+ TRAVRVTDQILLS S++++ + +VDRLK+E+++ L Q + S LNTQR G +KS+LNL+KRL LD EW + L + MD LP +LR +F+N +T F P L LF+ME ITN++Y +PS +SAMQ++ +T DIL+ LTEQPLP NRDFLV LP LL+ L++N RGI I S II +Y+ TL + ++T M+ KR+R+ ++ + + ++ T+ MSNA+ EL R++ KP++++ + + V N+G K PA LL N P S + ++N I E V +I+ G S +S +++ + + V E D ++ +SG ED+ D++ ID + R +P G G + + + S +D ++L D++ N+CKF+E+ + ++ Q S+ GL+ + D++ +P LPYDFP S +C S+ KIFE L + D I + F+ + D L+ L Q + SILL++Y++ E +LH+LV +S+V+ I ++ + + +R + L G + RL ++WEA +++ +LF D++ D+
Sbjct: 1 MKIDRKKTVNRCGYDQNECMLLAKHLSVCPDDQLISELKQINVWNYGKCELGLWADVLDRLDTILENAVTKVGKWMLRLDAPGNSSLVDDVVTILEFTGHLIEHSIYRYLYGSWTHILALFGSSNLDVLLAVLGLSYNFSKRSNYFIRLDPSNKQMILDRLVSIAETWGGAENGFGLAACCDPKHFPETAGNVYFEYRSEQPLSRSSAPQ---NLPSPSDQTKSLNSSGTVILQALHRRNQTPSEIMEELLAIHPVP-ASQQMALFSRVRLATYFADPSQRHKCIRARLQALSILSYTFEVDERHLYPSLLDELVEVLGLPDDQYMGIKACALRTMTAIVNSHRLGINWGSLVGSTGLSSYHGILPNLTRKWIQGLVDGRIKAPAGSTNQHFTTALLSFLYHLACYEENVAPGNTQNTTLSSSGVLDSMTQLISWHTPHNDYLSYVTRAVRVTDQILLSI-----SSSRQHVVSILVDRLKYEVEVVL-----QSSTSSDTLNTQRSGLMKSILNLLKRLCLDAEWGDALHSVMDDNLPSVLRQVFLNGSTHFTPHLSLFAMETITNYLYTYPSRISAMQDKNITSDILKALTEQPLPQNRDFLVQLPALLNTLALNSRGIEAILSSGIISRYLNTLVSTEYLTTMKTKRVRDFISQIPYDMNCNSGQNLSNSLTASLMSNAIQELLRSH-TELKPVVFKCVISCLRHVVNLG-KTPATLLSENVSCNPAVYNILPSSTRDSVNF---------------GIRSFAEVVSEPVHHRTQISPDCGTSQPTTSAPLSLSNIQSAI-GVNEDD-----VVMLSGGEDE---DDDYNSDIDGNQGRQASPRSGTVQGGTMVSGALVSMISGLGSSSSKCENVSLVNDPSNTIRNESCALVTPNLFLSDFMLNLCKFLEKLLPFGSHTTDAMYPQFVSQGGLQVLCDLIHMPGLPYDFPVSASCASLCKIFEDLANSVNLTDVI---KPLFQFLESSLDMLRSLHDSQYPVTSILLREYLSGERE---------------------MLHELVTISSVVCILVQMIEHIKTEIRLPLANSWLTQGCLKTLGRLYLGVSWEAGIILKVLFGDKDITESDM 1049 HSP 2 Score: 316.62 bits (810), Expect = 5.310e-82 Identity = 206/616 (33.44%), Postives = 321/616 (52.11%), Query Frame = 3
Query: 10026 PLSITKEKSETKAV---NVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISS----DILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKV---DNSILTQFQSPSS-------------IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCH----------------------SDCS------------------EIHEEHNKSDNSNVSDPLSHL----------------NTSLVVDDTFMNSPAFSENGK---HNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDS--RYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQ 11618
P SI++ + +K + ++S+ +N + + I L ++ A P ++ R+ + +L+ +L++ N K+ I+RLL+ + ++ ++ L ++ E + + + P + P S+ + IS D+L + S+ SR ++ F+ L++ S + FT D L + L+ +V D+S++ P S W+ L Q + +Q + +AV++ Q L+E F L H + CS I ++ S+ +D L+HL L V D+ +++ + + NP +EF +K+++ L+ +LR+ A + +S F+ L YP++LDFD+KRR+F+ ++ L N S RY+++ + VSR R+FEDSY RLHR++ EWK +F I+F NEEGQD GG LREW+L MS EIFNPNY LF+VSP D VTYTINP+SY+N+NHLSYFKFVGRFIAKAI DNKLL CYFTRAFYKHILG+ +R +DLE EDY+FF+GLEFLL ++VS + + TFS +
Sbjct: 4081 PTSISENQLSSKHLPMSSLSNNLMNPLRPQVIETLCQLVLA-------PKFSETARIMTTQLITDLAQANPYMKELILRLLSITVGHLIANISNQLQCLVSE---VDRRLKDSISNSPPKSDSYKP--SSSRYPSEISRISSYDVLPDRFGIPGQMVVVSSDSRAQSSK---------------FSDLQLASSEAFTCPNNDQCRLRGILGLMLRVAAGDSSLVGSNPIPVSSDDLLNSVPGVNEFWNYLCQAFEKLQALNDQNAVLLLQPLLEVFCLAHVHLVKDTILLRQRPTNSRSNRTNSCSPGTLSFIDMVPLLHMCVDSISHSELDANRSSTTDHLAHLYFDLGGPQSPNVTATGEDDLTVTDSVVSASCTTASQSPFSRNPIIEFADKYRRGLSQVLRQHGA-NIGESPFAVFLAYPRVLDFDVKRRFFRQQLQSLSNRSTSSNRYDDDPITVSRDRIFEDSYARLHRRSVSEWKHKFVIRFQNEEGQDAGGPLREWFLLMSREIFNPNYCLFRVSPADRVTYTINPSSYINSNHLSYFKFVGRFIAKAINDNKLLECYFTRAFYKHILGVPVRCSDLESEDYEFFKGLEFLLSHNVSDLGYELTFSTE 4668 HSP 3 Score: 166.392 bits (420), Expect = 1.745e-36 Identity = 183/669 (27.35%), Postives = 303/669 (45.29%), Query Frame = 3
Query: 7929 NFRRWIVEARTLYGREITDLVMLARARVYQSVYHS-------------------VSSKLTEA-------SVDEKEVVDSK------TNMVKTANSNPDNVDNERNSITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPS-ETMESRDEPIDNIE-IEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLT---NPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQL------TSGLSDLLSQMNRGRWDQR---NIRYVVFSER--GQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXIL---CTPIGSGSCTDMS-----RSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVP-----SISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFP-----------TSQSKKVP-TNFW 9716
++RRW +R L+G EI DL++L R +Y+++ V+S L E +V K +++S T + T S +N N + D + D S + + D+ + E I E+ + P T ++ +P + E I + + + DE+ +++ L+ G+DP+FL+ALP+ MR+EVI +HR A +V+Q++ + LP ++++E + LP HIQEE+LA +R+E + ++ T ++ P + S +++FL+SL PS+ RE+LADM++SQ+ L L EA ++R+E + +RA+Q +LS + R Q TSGL +GRW R N + VV G + +N AV T AG LS+ + +V +GLT +IALL+ SS+S + + I V K+L++L+CH R WI TI+ + +S KP + +TS S C PI S S + SS + + N +LF FE ++F P + S ++ I + IH + SVA L+++LS ++ F+P TS + P T+FW
Sbjct: 3244 HYRRWTNLSRMLFGHEIMDLILLGRHYIYKALEQKRHELFNLRIAENEIVCLPVVTSSLNEPLVSSPALTVSSKSIIESPETSVTFTTPLVTCISTSENTRNSQELSADLQPVAGDSSNAFN-QINSDVCVS---EAAIGSIASELSSSNPVFSTAQNFPQPTNTTENISNLVSSSNASNSDDNPTSATPTRSLVMTDEE----TIQSLVEGGMDPSFLDALPEEMRQEVIADHRYARQVQQRINSMGLPGHINSEWLTGLPPHIQEEVLAQFRQEQQQQQQSSTQPTTTSGETDTSEQPASTQSATDSNTAFLISLPPSVLREVLADMEESQLETLPQYLVVEANRLRREHEERYVRAVQNGILSHSNDPRPYQFWRNFTTTSGLFG----GGQGRWVVRFHSNDQIVVHPNNIDGPLSLNNALAVR---TMAG----LSS-REIVDHEGLTCIIALLVASSSSSNQRQNIVPTVKKVLRNLSCHSATRNWIVNTIIFLFNGLS-------EKPPPYLHFTTSCSMPFTEDCEPIPSTSTNQKTSFPGINSSENELQCSTKPTGNNTLFHTGFEAALGCWVRIFHPVPQNLNDSHTFESNYSKDISTAVPVRYIIHPQGAVSVAGVLLETLSELTRAFPSHFYPPDICQLSESAGTSHTTDTPSTSFW 3885 HSP 4 Score: 108.997 bits (271), Expect = 3.412e-19 Identity = 113/518 (21.81%), Postives = 212/518 (40.93%), Query Frame = 3
Query: 4395 REISTIEARLKELEMKNVSIKEMIKESFLINLFN-----LQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLI----LNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLL------------------------FKSFMAVPHQTETF-KTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLP-----SKSKDQRKFENLSEYEHKIISC-VPDPLGDEEVVELSDNMLQFL--------------ISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIEL---NCQTEKQVL-KTYSISKS 5774
+ +ST + L +E+ N +ES N+F ++ I+ +I ++ + +G+ ++ ++ ++ + I S L T ++ + E GLH++ LL+ + NH + + L++ + L F A H + K+ K +T +L++DQYER+ ALR+R++ + Y W W D++ +WHP + N I++S+ G L A N Y F ++TQ N S RP+ML P +++ + + + YE +++ +P L EE +L +L +S K++ DCL A LL+RLC ++ LA + L + PH + ++ + FV L+ + F+ + +I + G + + +DLFY + PLL ++ E ++ L + L + E +V KTY + +S
Sbjct: 1626 KRLSTSKFPLVMMELCNEKTYNESRESLKQNVFKTCYAIAKRHHSYQILHQIAELFISSGEHERYINELFSVVTSHLKSYLGITSTALETTKSSNNFDNESHGFTNHEKEHQTVGLHLVALLFTRCPLVCACLAWNHKLPSVLIQFVSDLDLLNTAAIGDDVALHEQTTMEVDGFSGGDASSHNSNHISKSDYYKTLTLAFLVLDQYERSIQALRLRQESVKLYASSHNWHWFDNQAMLWHPYTNDSNRIIDKSFHKGDLCAGCDIN-RRPYTVEFPTMTQINLVSMHRRPVMLFPPTNENTEADQETSSSHATSYELSLMNTEIPKCLSAEERSKLCHALLTHFRLFKNEPSSELHSSMSTKQLP--LSSDCLNAMFRLLLRLCFTSHENADELAE-ADILKTLFSYPHTREFSPEFSAFVGALISEIFDDASTTDRIMKEIFIRMSRSGMPNVFMGIDAGPRACRDLFYLLSLCAPLLAKNRERSLRLAVETFNLSLPDSDIESKVYPKTYIVEES 2139
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Match: A0A4S2M655 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002440 PE=4 SV=1) HSP 1 Score: 766.918 bits (1979), Expect = 0.000e+0 Identity = 439/1120 (39.20%), Postives = 653/1120 (58.30%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQ------SSVDV-----------FIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNE---VNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSV--ALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMP-----------------------LQENVDSQEIA-STAGPSGVYSSDSNQAADLLAV----------MTAVEEADREVEQIMEMSGDEDDGVADNEMTQ---AIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFM-SDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQ--LYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAE--LKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDL 3188
MKIDR+KI NRCG+DQ EC LA RLAA ++ LI+E+K+I+VW+YGKCELGLW DVLDRLD IL VTKVG W L+LD P N L+ ++ ILEFTGHLIEHSIYRYLYGSW HILALFG+ NMDIL A+LGL YNFSKRSNYF R+D N+K++LERL SIAE WGG ++ FGLA CC ++ P +AG + +E+ D S G +Q S D+ + PK K+ LR LH + +SPS++ME L+ ++ L S QMALFAR+RL YF PEQR I R+QALSIL YT +V+++ L +++ELV+VL+LPD ++ GIKACALRT+TAI++ +L W L + TG++SYHG P++ R WI LV + + Q +TTAILSFLYHLAC E +T L +G+LD+M ++I WHT D +S+ TRAVRVTDQIL+S +T++ + ++ +V+RL FE+ + L + D ++ + ALNTQR G +KS+LNL+KRL LD +W E + M+ LPDILR +F+N + F P L LF+ME ITN++Y +PS +S+MQ++ +T DIL LT QPLP NRDFLV LP L + L++N RGI I+ S ++ KY++TL ++ M+ KR+R+ ++ + +++T S ++ T+ QMSNA+ EL R+ + + P+ ++ + K V IG+ QPA L+ + ++ H + DD+ +P L ++ + ++A ST+G G+ +D D++ + +++ R + + + D+ T +++ +ED P++ + S +L D++ N CKF+E+ + S ++ + S GL+ + D+L +P LPYDFP S +C S+ KIFE L + ND I F+ + ++L L+ Q L S+LL++Y+ N LLH+LV+ +++I I +I D L + LR + + +++ + RL ++WEA +L+ +L+ D++ D+
Sbjct: 1 MKIDRRKIVNRCGYDQNECMLLAKRLAACPDDTLISELKQISVWNYGKCELGLWVDVLDRLDAILEHAVTKVGRWMLRLDLPENASLVDDVVTILEFTGHLIEHSIYRYLYGSWNHILALFGSENMDILLAVLGLAYNFSKRSNYFVRMDHHNKKMILERLVSIAETWGGSENNFGLAACCDPDHFPETAGNIYFEYRPDSKDGSFIGGNPPSQLETETTPSGDIAEHTDGCGNVEWTAPK----KIVLRALHRRNQSPSQIMEELLSQH-LLPPSKQMALFARIRLATYFAIPEQRHKCIRARLQALSILSYTFDVDERHLYPSLLDELVEVLELPDGQYTGIKACALRTMTAIVNSHRLGITWTSLVSCTGLSSYHGILPSLTRRWIQGLVDGTIEAPGGSTNQQFTTAILSFLYHLACYEENVGPGNTQNATLSSSGVLDTMLQLIAWHTSRNDCLSYVTRAVRVTDQILMSI-----ATSRLNVVNILVNRLSFEVDLVLKASTDGKIQQDLGPALNTQRSGLMKSILNLLKRLCLDTDWGEVMHTVMEDTLPDILRQVFLNGSIHFTPHLALFAMETITNYLYTYPSRISSMQDKNVTTDILTALTSQPLPQNRDFLVQLPGLFNTLALNARGIEAIQSSGVLSKYLETLVSPDYLPTMKTKRVRDFLSQISYNLSSSTGQSLSNSLTASQMSNAIQELLRSRSELKTPV-FQGLVACMKRVVEIGRMQPASLVVEPV-----RCISDTHT------LGTDDDAKLPSEPPDSTVCEHLGSDLPCLTGVALGRSIGTSQVATSTSGGQGIGETDPMLEEDVVMLSGDEDEDGDDDHDIDDVSRATQTAAHQHRSANTRLMDSRSTSPTASLNLKEDLAPTN-----RPITSLADGSPVPAYHFLSDFMLNTCKFLEKLLPSVLHVTEAMCRHFMSHGGLQVLCDLLRMPGLPYDFPVSASCASLCKIFEDLANSIYLNDVI---APLFQCLESSLEKLAPLQNSQFTLTSLLLREYM---------------------ANEHVLLHELVVTASIICIMVQIIDHLKSELRPSLADKWLVRNVLHSLGRLYLGVSWEACILLKILYGDKDITEADM 1069 HSP 2 Score: 667.922 bits (1722), Expect = 0.000e+0 Identity = 385/890 (43.26%), Postives = 529/890 (59.44%), Query Frame = 3
Query: 9804 DYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFS-MKEKPVAAEWSIATNDGDNHESST------PTASTGN-ETPLSITKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKV---------DNSILTQFQSP-------SSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLC--------------------------------HSDCSEIHEEHNKSDNSN--------VSDPLSHLNTSLVVDDTF--MNSP--------AFSENGKH-----------NPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQL--RNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASM-STNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
DYF+ + L+ PL+Q+ + E++L+ + ILK + + M E+ ++ T + S P A G E+ + +E ++T + +V + + + + L +C +P T+ R + +L+ +L+ N + K+ ++ LL ++ ++ L+ L + DE L+++ + + +K S++ + +S D+L S S + VS G SR N P AF L+++SI+ FT + L + L+ ++ + S+ Q P S W LS V + +QK + +AV++ Q L+E+F L HS I + D+S V +PL + NT+ + D M+ P AF ++ N L+F E+H+ LN +LR+ +L +S F+ L YP++LDFDIKRR+F+ ++ L R+ +RY++E + +SR RLFEDSY RLHRK+ EWK +F I+F NEEGQD GG LREWYL MS EIFNPNY LF+ SP D VTYTINP+SY+N+NHLSYFKFVGRFIAKAI+D+KLL CYF+RAFYKHILG+ ++ +DLE EDY F++GLEFLLKN VS + + TFS +I+EFG+TETRDLI NG + VTE+NK EYVRLVCQE+MTGAIRQQL +FL GFYEIIPK +ISIFNE+ELELLI GLP ID+ DLK NT Y+KY P SPQI+WFW ALESFDQEDRARF+QFVTGTSKVPLGGF+ LEGM+GPTKFQIS AS+ STNHLP AHTCFN++ LP YE +++L LLTAIRECSEGYGM
Sbjct: 3825 DYFSQMADLLCHPLLQNRPSNQERVLTILAGILKEFIVNRGRMDEQSATTGQTVETTATLSQPSEQLEQPIPPEAVVGQPESSATGVQEPTKTSSASVPPDFVGSIRPEALKTLCEFVC-------SPKSTESGRSMAAQLITDLAHTNRDMKELMLNLLAESLNTLIGKLSVQLQEFSDELTSQLKSDTVISLKQKERA-----MKPSSSISRLVSMDVLPDRFSSSGQTVV-----------VSGGDSRPN--PATAFCDLQLKSIQAFTCPNNEQSRLRGIVGLMVRLTAGECSLNANASVSVSNQDPFASITSLSGFWPKLSSVFEKLQKCPDMNAVLLLQPLLEAFCLAHLHLVKDSIMIRQRSATSRTNRPTITVTASGAHSLVDMIPMLQVRVDSSTPSASERDEVREPLINENTTHLQLDVMGPMSPPTLSTEDDKAFGDDATRCRRGSSNSATANAILQFAEQHRIGLNQVLRQHGG-SLGESPFAVFLAYPRVLDFDIKRRFFRQQLQALSHRSPLSNRYDDEPILISRDRLFEDSYARLHRKSPAEWKHKFVIRFHNEEGQDAGGPLREWYLLMSREIFNPNYCLFRTSPADRVTYTINPSSYINSNHLSYFKFVGRFIAKAIYDSKLLECYFSRAFYKHILGVPVKCSDLESEDYDFYKGLEFLLKNHVSDLGYELTFSTEINEFGKTETRDLIENGRNVPVTEQNKKEYVRLVCQERMTGAIRQQLDAFLAGFYEIIPKRMISIFNEQELELLISGLPNIDIGDLKANTTYSKYHPNSPQIEWFWCALESFDQEDRARFLQFVTGTSKVPLGGFANLEGMHGPTKFQISRASVSSTNHLPCAHTCFNTLVLPAYETYEQLRARLLTAIRECSEGYGM 4688 HSP 3 Score: 193.741 bits (491), Expect = 8.722e-45 Identity = 179/664 (26.96%), Postives = 296/664 (44.58%), Query Frame = 3
Query: 7905 FNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSI----TDQTDI-GRDVSMEQDLMMQPDIMIESLDE-------PNESVIGEEMVTPTPSETMESR-------DEPIDNIEIE--QPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYR--------------RENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVT--DPVTEAG--EWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVP--------------SISKIYKHKN-----------KSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVP 9704
+ ++ ++RRW+ +R L+G E+ DLV+++R +V+Q + L ++ + + + + + S P+ + S + Q ++ G ME + P I + L E P+E ++ T ++E+ D+ I E Q S T DE+ +++ L+ G+DP+FL+ALP+ MRREVI +HR A +V+QQL I LPE+++++ +A LP HIQEE+L +R +TS A + + T ESGS+ + S +++FL+SL PSLRRE+LADM++SQ+ L +L EARQ+R+E+ + RA+Q +L+ + R +L G GRWD R FS RH N+ P T G P + + LV +GLT L+ALL+ SS+ + + V K+L++L+CH R WI T++ I +S + + KP + S S+ P S S + + + NN +F FE +VF P SKI + + +G + M IH + +V+ L+D+L ++ F+P + P
Sbjct: 3074 YAGYALPASYRRWVTLSRLLFGHELMDLVLISRYQVHQELSRRRQDTLKRRILEAERLANQQQATLAATPSQPEVALSAVTSTATIESHQPEVYGAPAQMETSHLPTPPISNQPLVEHTLQYSQPDEGTTAPQVETAALPPSIEASYGSPTVADDTAPGISEEPVQATTTTSTGGSGQPTQSQPTPPPVPMTDEE----TIQSLVESGMDPSFLDALPEEMRREVIADHRHARQVQQQLRSITLPEHINSDWLAGLPPHIQEEVLLQFRNEQQQNAAAAASGANTTTTSGAAESAATTVNESGSIVRVPES----NTAFLISLPPSLRREVLADMEESQLEMLPPDLVAEARQLRRESEERFARAVQGGMLAHSFTSRHDRLIGG---------PGRWDVR------FSSINHFLRHLNSLTGPFPPNTSVGLHNLPGIRG-RHLVDYEGLTCLLALLVASSSCSQQRRQLIPTVKKVLRNLSCHFATRNWIVGTLLCIFGGLS--ENCSKEKPPL-----ESAPSLTLEPQPSTSVAQRDSEVKLLRQSKPCFSNN--IFHTGFEAALGCWVRVFHPVSQPIQSFPPETEEGQSKISQESDIGEPLTFYCEPTAGTSATTMRNHMIHPQAAAAVSNVLLDTLGDLAKAFPAHFYPRLSGDQKP 3704 HSP 4 Score: 113.235 bits (282), Expect = 2.031e-20 Identity = 121/520 (23.27%), Postives = 220/520 (42.31%), Query Frame = 3
Query: 4416 ARLKELEMKNVS-IKEMIKESFLINLFNLQKEKGID-IITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPN-GLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFK----TSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENL-SEYEHKIISCVPDPL--GDEEVVELSDNMLQF---------------LISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKS-----------AIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQ----TEKQVLKTYSISKSPSLTSKATQPLMELEKKQIAIVRFLVE 5855
A L+ L + + ++E +K+ +++ K D I+ RI ++LL +G+ +++++ + ++ +S T+ + +G ++++ P GLH+ LL+++ + H++ L+ +S P T+ T K + LI+D YER+ A+++R+ Y TW W DDR +WHP +++ I+ ++ NG+L AY S Y F ++TQ N +S RP++L+PS ++D + N S YE I+ P+ G + + + QF L + NV+ A+ L C + AY S + L PH + +T + FV +++ Q FE + T+ +++ K G N +DLFY + P++ +D ET + + I L+ T K V K Y I ++P Q L +Q AI+ L+E
Sbjct: 1588 AELEPLAPEKIEELRESLKKHVFRACYSIAKHHHSDQILHRIAELLLSSGEEEHYIDELFGFVAAT-MSGRLGFSAASPTIPSLSVNVDEAGVSVQEQPTVGLHLSALLFSRCQSLCARLAWKHNLPHLLITWVARCGQSADRPPDTTQQANHGPDTDYYKTLLLSCLILDLYERSIQAMQLRQISSKLYLNSHTWNWFDDRAMLWHPYASESGRIIDTAFHNGELSAYCH-ISRRPYAVEFPTMTQINLDSMHRRPVLLMPSTAEDTDERSNKESAYERSILDAEIPPMLTGKQRRTLFTGLVHQFERATTHATITQTTVPLCDVSCPTNVH------ASPVLFPSDCVNAMLRLLLRLAYSSFDDAREMIDVNLLSVLLAFPHSRDFTAEYSAFVGSIVIQMFEDRPTVNRTMKEVIKKMCKFGLPSSFMGVRINSPACRDLFYLLSMCAPIMAKDRETALKIACDTINLSLSDADVTAKTVPKDY-IVEAPLEPDDKDQDTFPLSDRQQAIITQLIE 2098
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Match: A0A4S2M6H5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002440 PE=4 SV=1) HSP 1 Score: 766.918 bits (1979), Expect = 0.000e+0 Identity = 439/1120 (39.20%), Postives = 653/1120 (58.30%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQ------SSVDV-----------FIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNE---VNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSV--ALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMP-----------------------LQENVDSQEIA-STAGPSGVYSSDSNQAADLLAV----------MTAVEEADREVEQIMEMSGDEDDGVADNEMTQ---AIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFM-SDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQ--LYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAE--LKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDL 3188
MKIDR+KI NRCG+DQ EC LA RLAA ++ LI+E+K+I+VW+YGKCELGLW DVLDRLD IL VTKVG W L+LD P N L+ ++ ILEFTGHLIEHSIYRYLYGSW HILALFG+ NMDIL A+LGL YNFSKRSNYF R+D N+K++LERL SIAE WGG ++ FGLA CC ++ P +AG + +E+ D S G +Q S D+ + PK K+ LR LH + +SPS++ME L+ ++ L S QMALFAR+RL YF PEQR I R+QALSIL YT +V+++ L +++ELV+VL+LPD ++ GIKACALRT+TAI++ +L W L + TG++SYHG P++ R WI LV + + Q +TTAILSFLYHLAC E +T L +G+LD+M ++I WHT D +S+ TRAVRVTDQIL+S +T++ + ++ +V+RL FE+ + L + D ++ + ALNTQR G +KS+LNL+KRL LD +W E + M+ LPDILR +F+N + F P L LF+ME ITN++Y +PS +S+MQ++ +T DIL LT QPLP NRDFLV LP L + L++N RGI I+ S ++ KY++TL ++ M+ KR+R+ ++ + +++T S ++ T+ QMSNA+ EL R+ + + P+ ++ + K V IG+ QPA L+ + ++ H + DD+ +P L ++ + ++A ST+G G+ +D D++ + +++ R + + + D+ T +++ +ED P++ + S +L D++ N CKF+E+ + S ++ + S GL+ + D+L +P LPYDFP S +C S+ KIFE L + ND I F+ + ++L L+ Q L S+LL++Y+ N LLH+LV+ +++I I +I D L + LR + + +++ + RL ++WEA +L+ +L+ D++ D+
Sbjct: 1 MKIDRRKIVNRCGYDQNECMLLAKRLAACPDDTLISELKQISVWNYGKCELGLWVDVLDRLDAILEHAVTKVGRWMLRLDLPENASLVDDVVTILEFTGHLIEHSIYRYLYGSWNHILALFGSENMDILLAVLGLAYNFSKRSNYFVRMDHHNKKMILERLVSIAETWGGSENNFGLAACCDPDHFPETAGNIYFEYRPDSKDGSFIGGNPPSQLETETTPSGDIAEHTDGCGNVEWTAPK----KIVLRALHRRNQSPSQIMEELLSQH-LLPPSKQMALFARIRLATYFAIPEQRHKCIRARLQALSILSYTFDVDERHLYPSLLDELVEVLELPDGQYTGIKACALRTMTAIVNSHRLGITWTSLVSCTGLSSYHGILPSLTRRWIQGLVDGTIEAPGGSTNQQFTTAILSFLYHLACYEENVGPGNTQNATLSSSGVLDTMLQLIAWHTSRNDCLSYVTRAVRVTDQILMSI-----ATSRLNVVNILVNRLSFEVDLVLKASTDGKIQQDLGPALNTQRSGLMKSILNLLKRLCLDTDWGEVMHTVMEDTLPDILRQVFLNGSIHFTPHLALFAMETITNYLYTYPSRISSMQDKNVTTDILTALTSQPLPQNRDFLVQLPGLFNTLALNARGIEAIQSSGVLSKYLETLVSPDYLPTMKTKRVRDFLSQISYNLSSSTGQSLSNSLTASQMSNAIQELLRSRSELKTPV-FQGLVACMKRVVEIGRMQPASLVVEPV-----RCISDTHT------LGTDDDAKLPSEPPDSTVCEHLGSDLPCLTGVALGRSIGTSQVATSTSGGQGIGETDPMLEEDVVMLSGDEDEDGDDDHDIDDVSRATQTAAHQHRSANTRLMDSRSTSPTASLNLKEDLAPTN-----RPITSLADGSPVPAYHFLSDFMLNTCKFLEKLLPSVLHVTEAMCRHFMSHGGLQVLCDLLRMPGLPYDFPVSASCASLCKIFEDLANSIYLNDVI---APLFQCLESSLEKLAPLQNSQFTLTSLLLREYM---------------------ANEHVLLHELVVTASIICIMVQIIDHLKSELRPSLADKWLVRNVLHSLGRLYLGVSWEACILLKILYGDKDITEADM 1069 HSP 2 Score: 667.922 bits (1722), Expect = 0.000e+0 Identity = 385/890 (43.26%), Postives = 529/890 (59.44%), Query Frame = 3
Query: 9804 DYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFS-MKEKPVAAEWSIATNDGDNHESST------PTASTGN-ETPLSITKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKV---------DNSILTQFQSP-------SSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLC--------------------------------HSDCSEIHEEHNKSDNSN--------VSDPLSHLNTSLVVDDTF--MNSP--------AFSENGKH-----------NPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQL--RNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASM-STNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
DYF+ + L+ PL+Q+ + E++L+ + ILK + + M E+ ++ T + S P A G E+ + +E ++T + +V + + + + L +C +P T+ R + +L+ +L+ N + K+ ++ LL ++ ++ L+ L + DE L+++ + + +K S++ + +S D+L S S + VS G SR N P AF L+++SI+ FT + L + L+ ++ + S+ Q P S W LS V + +QK + +AV++ Q L+E+F L HS I + D+S V +PL + NT+ + D M+ P AF ++ N L+F E+H+ LN +LR+ +L +S F+ L YP++LDFDIKRR+F+ ++ L R+ +RY++E + +SR RLFEDSY RLHRK+ EWK +F I+F NEEGQD GG LREWYL MS EIFNPNY LF+ SP D VTYTINP+SY+N+NHLSYFKFVGRFIAKAI+D+KLL CYF+RAFYKHILG+ ++ +DLE EDY F++GLEFLLKN VS + + TFS +I+EFG+TETRDLI NG + VTE+NK EYVRLVCQE+MTGAIRQQL +FL GFYEIIPK +ISIFNE+ELELLI GLP ID+ DLK NT Y+KY P SPQI+WFW ALESFDQEDRARF+QFVTGTSKVPLGGF+ LEGM+GPTKFQIS AS+ STNHLP AHTCFN++ LP YE +++L LLTAIRECSEGYGM
Sbjct: 3830 DYFSQMADLLCHPLLQNRPSNQERVLTILAGILKEFIVNRGRMDEQSATTGQTVETTATLSQPSEQLEQPIPPEAVVGQPESSATGVQEPTKTSSASVPPDFVGSIRPEALKTLCEFVC-------SPKSTESGRSMAAQLITDLAHTNRDMKELMLNLLAESLNTLIGKLSVQLQEFSDELTSQLKSDTVISLKQKERA-----MKPSSSISRLVSMDVLPDRFSSSGQTVV-----------VSGGDSRPN--PATAFCDLQLKSIQAFTCPNNEQSRLRGIVGLMVRLTAGECSLNANASVSVSNQDPFASITSLSGFWPKLSSVFEKLQKCPDMNAVLLLQPLLEAFCLAHLHLVKDSIMIRQRSATSRTNRPTITVTASGAHSLVDMIPMLQVRVDSSTPSASERDEVREPLINENTTHLQLDVMGPMSPPTLSTEDDKAFGDDATRCRRGSSNSATANAILQFAEQHRIGLNQVLRQHGG-SLGESPFAVFLAYPRVLDFDIKRRFFRQQLQALSHRSPLSNRYDDEPILISRDRLFEDSYARLHRKSPAEWKHKFVIRFHNEEGQDAGGPLREWYLLMSREIFNPNYCLFRTSPADRVTYTINPSSYINSNHLSYFKFVGRFIAKAIYDSKLLECYFSRAFYKHILGVPVKCSDLESEDYDFYKGLEFLLKNHVSDLGYELTFSTEINEFGKTETRDLIENGRNVPVTEQNKKEYVRLVCQERMTGAIRQQLDAFLAGFYEIIPKRMISIFNEQELELLISGLPNIDIGDLKANTTYSKYHPNSPQIEWFWCALESFDQEDRARFLQFVTGTSKVPLGGFANLEGMHGPTKFQISRASVSSTNHLPCAHTCFNTLVLPAYETYEQLRARLLTAIRECSEGYGM 4693 HSP 3 Score: 193.741 bits (491), Expect = 9.251e-45 Identity = 179/664 (26.96%), Postives = 296/664 (44.58%), Query Frame = 3
Query: 7905 FNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSI----TDQTDI-GRDVSMEQDLMMQPDIMIESLDE-------PNESVIGEEMVTPTPSETMESR-------DEPIDNIEIE--QPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYR--------------RENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVT--DPVTEAG--EWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVP--------------SISKIYKHKN-----------KSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVP 9704
+ ++ ++RRW+ +R L+G E+ DLV+++R +V+Q + L ++ + + + + + S P+ + S + Q ++ G ME + P I + L E P+E ++ T ++E+ D+ I E Q S T DE+ +++ L+ G+DP+FL+ALP+ MRREVI +HR A +V+QQL I LPE+++++ +A LP HIQEE+L +R +TS A + + T ESGS+ + S +++FL+SL PSLRRE+LADM++SQ+ L +L EARQ+R+E+ + RA+Q +L+ + R +L G GRWD R FS RH N+ P T G P + + LV +GLT L+ALL+ SS+ + + V K+L++L+CH R WI T++ I +S + + KP + S S+ P S S + + + NN +F FE +VF P SKI + + +G + M IH + +V+ L+D+L ++ F+P + P
Sbjct: 3079 YAGYALPASYRRWVTLSRLLFGHELMDLVLISRYQVHQELSRRRQDTLKRRILEAERLANQQQATLAATPSQPEVALSAVTSTATIESHQPEVYGAPAQMETSHLPTPPISNQPLVEHTLQYSQPDEGTTAPQVETAALPPSIEASYGSPTVADDTAPGISEEPVQATTTTSTGGSGQPTQSQPTPPPVPMTDEE----TIQSLVESGMDPSFLDALPEEMRREVIADHRHARQVQQQLRSITLPEHINSDWLAGLPPHIQEEVLLQFRNEQQQNAAAAASGANTTTTSGAAESAATTVNESGSIVRVPES----NTAFLISLPPSLRREVLADMEESQLEMLPPDLVAEARQLRRESEERFARAVQGGMLAHSFTSRHDRLIGG---------PGRWDVR------FSSINHFLRHLNSLTGPFPPNTSVGLHNLPGIRG-RHLVDYEGLTCLLALLVASSSCSQQRRQLIPTVKKVLRNLSCHFATRNWIVGTLLCIFGGLS--ENCSKEKPPL-----ESAPSLTLEPQPSTSVAQRDSEVKLLRQSKPCFSNN--IFHTGFEAALGCWVRVFHPVSQPIQSFPPETEEGQSKISQESDIGEPLTFYCEPTAGTSATTMRNHMIHPQAAAAVSNVLLDTLGDLAKAFPAHFYPRLSGDQKP 3709 HSP 4 Score: 112.849 bits (281), Expect = 2.246e-20 Identity = 121/520 (23.27%), Postives = 220/520 (42.31%), Query Frame = 3
Query: 4416 ARLKELEMKNVS-IKEMIKESFLINLFNLQKEKGID-IITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPN-GLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFK----TSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENL-SEYEHKIISCVPDPL--GDEEVVELSDNMLQF---------------LISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKS-----------AIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQ----TEKQVLKTYSISKSPSLTSKATQPLMELEKKQIAIVRFLVE 5855
A L+ L + + ++E +K+ +++ K D I+ RI ++LL +G+ +++++ + ++ +S T+ + +G ++++ P GLH+ LL+++ + H++ L+ +S P T+ T K + LI+D YER+ A+++R+ Y TW W DDR +WHP +++ I+ ++ NG+L AY S Y F ++TQ N +S RP++L+PS ++D + N S YE I+ P+ G + + + QF L + NV+ A+ L C + AY S + L PH + +T + FV +++ Q FE + T+ +++ K G N +DLFY + P++ +D ET + + I L+ T K V K Y I ++P Q L +Q AI+ L+E
Sbjct: 1593 AELEPLAPEKIEELRESLKKHVFRACYSIAKHHHSDQILHRIAELLLSSGEEEHYIDELFGFVAAT-MSGRLGFSAASPTIPSLSVNVDEAGVSVQEQPTVGLHLSALLFSRCQSLCARLAWKHNLPHLLITWVARCGQSADRPPDTTQQANHGPDTDYYKTLLLSCLILDLYERSIQAMQLRQISSKLYLNSHTWNWFDDRAMLWHPYASESGRIIDTAFHNGELSAYCH-ISRRPYAVEFPTMTQINLDSMHRRPVLLMPSTAEDTDERSNKESAYERSILDAEIPPMLTGKQRRTLFTGLVHQFERATTHATITQTTVPLCDVSCPTNVH------ASPVLFPSDCVNAMLRLLLRLAYSSFDDAREMIDVNLLSVLLAFPHSRDFTAEYSAFVGSIVIQMFEDRPTVNRTMKEVIKKMCKFGLPSSFMGVRINSPACRDLFYLLSMCAPIMAKDRETALKIACDTINLSLSDADVTAKTVPKDY-IVEAPLEPDDKDQDTFPLSDRQQAIITQLIE 2103
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Match: A0A0X3PN07 (Uncharacterized protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR125038 PE=4 SV=1) HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0 Identity = 490/1414 (34.65%), Postives = 750/1414 (53.04%), Query Frame = 3
Query: 12 LYE-MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQG-----NLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSE--IDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPS-GVYSSDSNQAADLLAVMTAVEEADREV-----------EQIMEMSGDEDDGVAD----------------------------NEMTQAID------AEEDRTPSDGCGKDNKTEEMNQFSCDDDAV----YLMDYICNVCKFMERFM-SDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLR---------QDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKR----LCINERGTCADNEHNEER------CFLTKKSS---LSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSXXXXXXXXXXXGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSW-PEDPHSELPVEKACQKILSDIKFL---HPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEP 3998
LY+ MKIDRKKI NRCG+DQ EC LA L+ L +++ I E+++I VW+YGKCELGLWAD+LDRLD IL T+VG WT+K+D P NE L+ ++ +LEFTGHLIEHSIYR LYGSW HIL LFG+SNMDIL A+LGL YNFSKRSNYFSRLD+ N+K++L+RL SIAE WGG + F LA CC + P AG + +E+ DV++ S E ++ +V + + +L ESPS++ME L++K+ + ++MALFAR+RL VYF PE+R I R+QA+S LCYT E+ D+ L +V+ELVDVL+LPD +F+ IKACALRT+TAI ++ + TGM+S+HG PT +R+WI LV +V Q YT A+LSFLYHLA E + G L +G+LDSM ++I WH D +S+ TRAVRVTDQIL++ ++TN+++ ++ +VDRL +E+ + L ++ +D +++ LNTQR G +KS+LNL+KRL LD +W++ ++ +M+G LP IL I+ N F P L LF+ME +TN++Y +PS +S MQE +T IL LT QPLP NRDFLV LP++L+ L++N RG ++ I++KY++TL +++ M+ +R+R+ + S + + T+ QMSN++ EL R++ + KP +++S+ + F+ V +G Q AP+L +P T+ +E+ + ST G + V+SS++ + + AV +A E ++ +SG ED+G A+ T ID +E T +DG D K +++ S DA L +YI N+CKF+ER + S Q + +Q ++ GL+ + D+LT+P LPYDFP S AC S+ KIFE L+S+ + ND + + + + D L K+ + L + S F D A E GV LH LV +S+++ + +I + L R ++IL +L L ++WEA++L+ L ++ + + L ++ T A+ + + + F+ S L C Q+ +++ + +L + N + EL TLT+FC P S R +P K ++L ++A+ L G W P D +L++ FL + ++Y H +L +K P + F AF M NG++ F N+L +L + ++ +LE + VDR++ I + E ++ + F+ + Y + L+P
Sbjct: 6 LYDLMKIDRKKIGNRCGYDQNECMLLAKHLSTLSDDEFIQELRRIKVWNYGKCELGLWADILDRLDGILENAATRVGAWTIKVDLPENEKLVEDVVTVLEFTGHLIEHSIYRCLYGSWNHILTLFGSSNMDILLAVLGLTYNFSKRSNYFSRLDAANKKMILDRLISIAETWGGSESHFDLASCCQPNHFPPMAGKIYFEYQPDVAN--SLNHEKKDEVAVVCPNTMSLSGPDVIIEDLKGCRESPSQIMEALLKKHPVP-LPNRMALFARIRLAVYFSDPEKRLKCIRARLQAISTLCYTFEINDRLLYPSLVDELVDVLQLPDGDFMEIKACALRTMTAIFSSHRVNVNLMSILESTGMSSFHGALPTRIRKWIQGLVDGSCDAAGGSVNQQYTIALLSFLYHLASFEENIGSGSGQNHATTLSTSGILDSMLQLIAWHVPRNDCLSYVTRAVRVTDQILMN-----AATNRQTVVNRLVDRLGYEVDLVLSNQGKLDATGGQALLLNTQRSGLMKSILNLLKRLCLDTDWNDAVRTSMEGNLPQILCSIYKNSVFHFTPHLALFAMETVTNYIYTYPSRISNMQENGITCGILSALTTQPLPQNRDFLVHLPSILNTLALNTRGRAALRSCGILNKYLETLVSPDYLSTMKARRVRDFHSQISYSLAPSAGQVFSTNLTASQMSNSIQELLRSHNEL-KPAVFQSLVECFRKVVRLGCSQSAPIL--------------------PSPETLGSSELESEGSCRSFNQSYSTHGRNLNVFSSENRPDSRMTVTSAAVIDATNPTANRTPGGVLLDEDLVMLSGSEDNGSAEDDDENDDMPDVGLTASRTAVSSSTIFRTTARTAHIDTTDSSVSESHSTTTDGDTPDQKPSALSEASISSDAPESFQNLPEYILNLCKFVERLLPSMQHVTEGMCRQFVNQGGLQVLCDLLTMPGLPYDFPVSAACASLCKIFEQLVSYLSLND------LLKPVLQALQDGLSKVS-----NFLASETTEYSLLFTDCSA---------NETGV--LHGLVCISSILCVLVQIINHLKPEHRCGFASLWAQRNILGQL-------GELYHKVSWEAVILLKALCDENKDLTQPGSSTSYPLPVDANETSANTQTAQSKPPFSMPAFVEALSVYRILKPCLQVHQPT-KTKSLAHYILHVCSYFINSVTELCTTLTRFCVASP--SQPQRRLLMSSFDQVPLSA-KTSVLTQVAQ-------------LAIGALCWRPPD------------AVLNNSDFLLFVYAVRYSSVRLAHLLLFDAS-SKSPQILMFNAFYMLNGIQSLFSIFNDLASFNSSTDFL----SGALSTVLEEWFACVDRLSNIVAVKAEVKKMSETDAV-HFNTNKYANYVNRLSLKP 1326 HSP 2 Score: 673.7 bits (1737), Expect = 0.000e+0 Identity = 387/881 (43.93%), Postives = 519/881 (58.91%), Query Frame = 3
Query: 9804 DYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPVAAEWSIATNDGDNHESSTPTASTGNETPLSITKEK------SETKAVNVSDPHLNRVNVKTISHL--------VNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKV------DNSI--------LTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDC----SEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFM-------NSPAFSENGKH-------------------------------------NPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRN-DGD-SRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMST-NHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
DYF+ L L+R P++ + EKLLS + +++ + K +P S T N E+ P ++ T + + + + AV P R+ K +S L + +C +++T T++ R S RL+I +++ N T ++ L + ++ L V++E PS + P T S S L+S P NA S R + + ++S+P ++L++ S++P T + + L + +L+ + D S + S +W LSQ LD ++ + + + V++ Q LVES L HS + S VS +S + L + SPAF N H +P E H+ LNHILR + L +S+F A L YP+ LDFDIKRR+F+ ++ + N DG SR+E+E V VSR R+FEDSY RLHRK+A EWK +F I+F NEEGQD GGLLREWYL MS EIFNPNY LF+VSP D VTYTINPAS++N+NHLSYFKFVGRFI KAI+DNKLL CYFTR+FYKH+LG+ +R+ DLE EDY F++GLEFLLK+DVS + D TFS +I+EFG+ ETRDLI +G I+VTE NK+EYVRLVCQE+MTGAIRQQL++FLEGFYEIIPK+LISIFNE+ELELLI GLP ID+ DLK NT Y+KY P SPQ++WFW+ALESFDQEDRARF+QF+TGTSKVPLGGF+ LEGM+GPTKFQIS A +S+ NHLP+AHTCFN+++LP YE++++L + LLTAIRECSEGYGM
Sbjct: 3710 DYFSQLASLLRHPVISCRPPLQEKLLSILVGVVREFCKRSRALHQP-----STTTAPLINRENVEPASAMAEGTTSEASSSQYAGGAMASSSAVGGDAPE--RIPKKPVSDLMLCPQHAVIECLCELVVAAKT---TEQARSLSVRLVILIAQANAKTNAQMLHYLCKAASDLSAIISRQLKAVINEV-----------DSLPPSNKASLPSSSKTTIPLSASMTRLSSVSDPEVSNA-GDPSDRFLAPTLVASLPSSSSVPPATASELELSSLQPLTTSRSEQSRLCCVLHLILHMLSCGSEDTSTNDAHTKVTMESINGLSQLWEDLSQTLDRLETTNDANTVLLLQPLVESLCLAHSTVLRENGSVRRLSRVSTIGVVSSGISFIPNLLPPEADLYELPPITNTSPAFGRNSTHVDSEPLSPASTIDLNRPMSPPLSTLNNATDAVSETASSPIAHLAETHRASLNHILRNFSG-NLSESSFVAFLSYPRALDFDIKRRFFRQRLQAIANRDGSVSRFEDEPVVVSRGRIFEDSYARLHRKSAAEWKHKFVIRFQNEEGQDAGGLLREWYLLMSREIFNPNYCLFRVSPADRVTYTINPASFINSNHLSYFKFVGRFIGKAIYDNKLLECYFTRSFYKHMLGVRVRFVDLESEDYDFYKGLEFLLKHDVSELGYDVTFSTEINEFGKNETRDLIEDGRNIIVTEANKNEYVRLVCQERMTGAIRQQLNAFLEGFYEIIPKSLISIFNEQELELLISGLPNIDIEDLKANTTYSKYQPNSPQVRWFWRALESFDQEDRARFLQFITGTSKVPLGGFANLEGMHGPTKFQISRAVVSSINHLPSAHTCFNTLELPPYESYEQLRERLLTAIRECSEGYGM 4567 HSP 3 Score: 171.4 bits (433), Expect = 5.111e-38 Identity = 146/494 (29.55%), Postives = 238/494 (48.18%), Query Frame = 3
Query: 7914 ISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTE----ASVDEKEVVDSKTNMVKTANSNPDNVDNERN------SITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNE---SVIGEEMV--------------TPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNP-ESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVT---DPVTEAGEWPNLSAY-KPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKIS 9299
S++TNFR I R LYG+E+ D+++L+R Y+ H++ + E + + V ++ + T + DN + S DQ +G S ++ + EPN+ SV E + P+ SETM P EQP ++ + + + + D ++ ++AEG+DP+FL+ALP+ +RRE++ +HRR ++R QL+ +QLPE+V AE ++ LP +IQEE+L R+E ++ N +G +++T +++FL SL PS+RRE+LADM+ QI L +L EAR +R+E RA+Q S+ G R R T+ LS GRWD R Y + R+ A V P +G S + L+ +GLT ++ LL+ SS P + + ++L++L HP IR WI CTI+++L ++S
Sbjct: 2983 FSILTNFRHLIDVCRILYGQEVMDMILLSR---YEVGVHAIRRRGDEFRERLAAHQASVNNAAASAETTTEATADNSSAAAHVPLLAPSTEDQAFVGAAASDDRYIRASVQSEWAPPHEPNDLASSVRPEASMEHQSASQSEREVAEPPSSSETMSVPSNP------EQPTAGDAQSNVPTPSAAPPLIVTEEIHVPVTDEETIASMVAEGMDPSFLDALPEDVRRELLADHRRTRQLRSQLSSMQLPEHVSAEFLSSLPPNIQEEVLTQVRQETQRQTGSDETQGQNTARAGEAANGAAATPENNAAFLASLPPSVRREVLADMEDFQIDLLPPDLQQEARTLRREHEAQFARAVQGR--SLPGLFRPRLDTTYFWQTLSGF--GRWDSRAGSY------NRLIRNGGAPVLGSRGPSAFSGLQSFFSGRNRHLIDHEGLTCILTLLVVSSACPVQRRHTVPALKRVLRNLASHPGIRNWIICTILSLLKRLS 3457 HSP 4 Score: 102.064 bits (253), Expect = 4.233e-17 Identity = 118/510 (23.14%), Postives = 214/510 (41.96%), Query Frame = 3
Query: 4458 EMIKESFLINLFNLQKE-----KGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVP-----------HQTETFKTS----VLKHITQLWL----IIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSK----SKDQRKFEN----LSEYEHKIISCVPDPLGDEEVVELSDNMLQFLIS--IKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQV-----LKTYSISKSPSLTSKATQPLM----ELEKKQIAIVRFLVES 5858
E ++ES ++F+ E K +I+ +I +++L + Q + +E ++A + S L L+ S + D+ GLH++ LL+ + RF + ++ L + + + P + + +S L+ L++ I+DQYER A+R+RK + Y+ C +W W D+R +WHP + + ++ NG +A + N Y F ++TQ N S RP++L+P S D + +++YE II+ P D +L ++ L+ + + +CL + LL+RL S A + L + H ++ Q FV ++RQ F+ + T+ IL K ++G C +DLFY + PL+ + E + LM + L EK + K++SI L + A ++ EL +Q IV F++E+
Sbjct: 1541 EKLRESLRKHIFSACYEIARLHKTDEILHQISELMLCSKQETQCIENLVAAVTTNLFAS-------LGVLLPTPSSP------VTDTGVGLHLIALLFTRCRFVCAQMVHKYSLILVLTALITDDVDTLRSHPLPVLLNLGESSAKPSAYMSSQSLVALEGDEDLFMLAAIILDQYERAVAAIRLRKKSVELYKDCHSWSWFDERANLWHPQAPDLFQAADAAFHNGATRAQWSID-RNRYAIEFPTMTQVNLSSMHRRPVLLMPCSLSPTSSDSPRPTGSTTQVTDYEGMIINMEPP---DGLSADLRRSLCLALLRRFDRSTEKPLSAECLNSFLRLLLRLT-ASSFEDAETVANADGLRVLFALSHPFEFSSQYSAFVGHIIRQLFDDLSTVTVTMQQILHK-FAEGVC----------SSCRDLFYLLSLCAPLVAKAEELAIRLMKETFSLVVD-EKSIEDGVPPKSFSIVN--PLETPANDAIIDVSPELNNRQKHIVVFIIEA 2018
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Match: huwe1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-081104-387]) HSP 1 Score: 373.629 bits (958), Expect = 6.320e-103 Identity = 258/799 (32.29%), Postives = 402/799 (50.31%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECC----VTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNM---TGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEI----KIC-------------------LDSEIDQM----------------------TKKSVALNT---------QRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKA 2204
MK+DR K+ +C L +L + L+ E++ I W+ GKCEL W D+LDR D IL + V + W L D+P N L ++A+L FT LIE+S R+LY S EH+ L + +M ++ ++L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC +T+Y P SA T+ +EF + SS K+ S+ TL +H++ ESPSE+ME+L YN+ Q LF +RL F ++R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + P L N+ TG SYHG P ++R I ++ +PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ K C +D+++D +S A++T QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG++ + + ++ K L ++ AMR +R + + + ++ + +A++EL R + K +I K + + N+G+ AP KP SIQKA
Sbjct: 1 MKVDRSKLKKTPTEAPADCRTLIEKLKGCSDEQLLLELQHIKTWNIGKCELYHWVDLLDRFDGILCDAGQTVENMSWMLVCDRPDNGQLKALLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASCDMQVVLSVLNLLYVFSKRSNYITRLGSDKRSPLLARLQHLAESWGGKENGFGLAECCRDLPMTKY-PPSATTLHFEFYAEPSSEVKVEKKSSSN----------------TLHYIHIEQLDKISESPSEIMESLTVMYNIPK-DKQTLLFTHIRLAHGFSNHKKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLVDIKAASLRTLTSIVHLERT----PKLSNIIDCTGTASYHGFLPVLVRNCIQAMIDPLMEPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFICRLEHEVDLSRKECPFVIKPKIQRPSTATETEDMDTDMDMSEVAMESSPGPSTSAGSRAETDPRAQSSAVSTPRTGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLHSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASN-----------------LGSAVDELMR-HQPTLKTDATTAIIKLLEEICNLGR---APEYICQKP-SIQKA 743 HSP 2 Score: 59.6918 bits (143), Expect = 9.827e-8 Identity = 81/327 (24.77%), Postives = 117/327 (35.78%), Query Frame = 3
Query: 4875 KHITQLWLIIDQYERTAFALRIRKDV-IYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML-------LPSKSK------------DQR-KFENL-----------------SEYEHK-IISCVPD-PLGDEE---------VVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQ-----LVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFE 5687
K IT + L+ID YE+ A + + R + Y W W DDR G W S +N+ I+ ++R G+ Y F ++ Q NEE+ RP+ML +P +K +QR K E E E K S VP P ++ V LS+ M LI + I D L A L +RL R Y L + + G TQ V L R E P L+HT+ ++ V+ GA S+++ Y + P R+ E
Sbjct: 1655 KWITPVLLLIDFYEKMAVSSKRRAQMNKYLQPNGNNWRWFDDRSGRWCSYSASNNSTIDSAWRAGETSVRFT-AGRRRYTVQFNTMVQVNEETGNRRPVMLTVQRVPRMPKPTKAGTAADSEREEGEQRAKTEETQTDTDSTSVAVEMAPPKEEAEQKESSSSVPTLPAASQDASSGSGAIVVQGLSEEMTTVLIRACVSMISVPVDPDTLHATLRLCLRLT--------RTHHYAMMFAELKSTRMILGLTQSSGFNGFTPLVTLLFRHIIEDPATLRHTMEKVVRSAVTSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPE 1972 HSP 3 Score: 58.151 bits (139), Expect = 3.022e-7 Identity = 27/71 (38.03%), Postives = 47/71 (66.20%), Query Frame = 3
Query: 2562 VYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLS 2774
+ LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+
Sbjct: 797 IPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVSILGLPNLPIDFPTSAACQAVAGVCKSILT 866
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Match: hace1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103021726]) HSP 1 Score: 331.643 bits (849), Expect = 4.275e-95 Identity = 182/459 (39.65%), Postives = 272/459 (59.26%), Query Frame = 3
Query: 10878 SLVVDDTFMNSPAFSENGKHNP-FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLD----------FDIKRRYFQAKVAQLRNDGDSRY----EEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNK-YTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
S V+ +M G +P F+EFV KH +VL + R + +F LL+ P+L+ F + +F + + D D + E +++ + R +F S + + T E+ KQ ++F EEG G G++REW+ +S+EI NP+YALF S DG T+ N S VN +HL+YF+F G+ + A++ +L+ YFTR+FYKHILGI + Y D+ D ++ + L+++L ND+S + L+ TFS++ FG E L P G+ I VT+ENK+EYV+LV + +MT AI+ Q+++FL+GF+ IP +LI +F+E ELELL+ G+PEID+ D K NT Y Y P IQWFW +ES QE+R +QFVTG+S+VP GGF+ + G +G KF I+ ++N LP + TC N + LP+Y + D L LL A+ S GY M
Sbjct: 407 SAVIQAFYMCCSCQMPQGMTSPRFIEFVCKHDEVLKCFVTRNPKIIFNHFHF--LLECPELMSRFMHIIKGQPFKDRCEWFYEHLLAGQPDSDMVHRPVNENDILLIHRDSIFRSSCEVVSKSTNEKLKQGIAVRFHGEEGM-GQGVVREWFDVLSNEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVSSIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVALKPGGTSIQVTQENKAEYVQLVTELRMTRAIQPQINAFLQGFHTFIPPSLIQLFDEYELELLLSGMPEIDVLDWKRNTEYTSGYDTEEPVIQWFWDVVESLTQEERVLLLQFVTGSSRVPHGGFAFIMGGSGLQKFTIAAVPYTSNLLPTSSTCINMLKLPEYPSKDVLRDRLLVALHCGSYGYTM 862
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Match: hace1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103021726]) HSP 1 Score: 332.028 bits (850), Expect = 1.290e-94 Identity = 182/459 (39.65%), Postives = 272/459 (59.26%), Query Frame = 3
Query: 10878 SLVVDDTFMNSPAFSENGKHNP-FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLD----------FDIKRRYFQAKVAQLRNDGDSRY----EEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNK-YTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
S V+ +M G +P F+EFV KH +VL + R + +F LL+ P+L+ F + +F + + D D + E +++ + R +F S + + T E+ KQ ++F EEG G G++REW+ +S+EI NP+YALF S DG T+ N S VN +HL+YF+F G+ + A++ +L+ YFTR+FYKHILGI + Y D+ D ++ + L+++L ND+S + L+ TFS++ FG E L P G+ I VT+ENK+EYV+LV + +MT AI+ Q+++FL+GF+ IP +LI +F+E ELELL+ G+PEID+ D K NT Y Y P IQWFW +ES QE+R +QFVTG+S+VP GGF+ + G +G KF I+ ++N LP + TC N + LP+Y + D L LL A+ S GY M
Sbjct: 476 SAVIQAFYMCCSCQMPQGMTSPRFIEFVCKHDEVLKCFVTRNPKIIFNHFHF--LLECPELMSRFMHIIKGQPFKDRCEWFYEHLLAGQPDSDMVHRPVNENDILLIHRDSIFRSSCEVVSKSTNEKLKQGIAVRFHGEEGM-GQGVVREWFDVLSNEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVSSIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVALKPGGTSIQVTQENKAEYVQLVTELRMTRAIQPQINAFLQGFHTFIPPSLIQLFDEYELELLLSGMPEIDVLDWKRNTEYTSGYDTEEPVIQWFWDVVESLTQEERVLLLQFVTGSSRVPHGGFAFIMGGSGLQKFTIAAVPYTSNLLPTSSTCINMLKLPEYPSKDVLRDRLLVALHCGSYGYTM 931
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Match: itchb (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:103022206]) HSP 1 Score: 327.405 bits (838), Expect = 1.696e-94 Identity = 170/408 (41.67%), Postives = 252/408 (61.76%), Query Frame = 3
Query: 10995 RRTACALD-QSNFSALLKYPKLL---DFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
RRT +D ++ S+L P++ DF K +YF+ QL + + + V+R LFEDS+ ++ +A++ ++R I F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F++ FYK IL + DLE D +F+ L ++ +N++ L+ FS+ G+ T +L P G I VTEENK EY+RLV + +++ + +Q +F EGF E++P+ + F+ KELE+L+CG+ EIDL D + NT+Y YT + QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I + N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 378 RRTTTYIDPRTGKSSLENGPQITYVRDFKAKVQYFRFWCQQL-----ALPQHIKINVTRKTLFEDSFQQIMSFSAQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSQPFYKRILNKPLGLGDLESIDPEFYNSLIWIKENNIEECGLEMFFSVDKDILGEITTHELKPEGGDIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVLLCGMQEIDLADWQRNTIYRHYTRGNKQIVWFWQLVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIE-KTGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 778
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Match: itchb (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:103022206]) HSP 1 Score: 328.561 bits (841), Expect = 5.663e-94 Identity = 170/408 (41.67%), Postives = 252/408 (61.76%), Query Frame = 3
Query: 10995 RRTACALD-QSNFSALLKYPKLL---DFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
RRT +D ++ S+L P++ DF K +YF+ QL + + + V+R LFEDS+ ++ +A++ ++R I F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YF+F+GRFIA A+F K + F++ FYK IL + DLE D +F+ L ++ +N++ L+ FS+ G+ T +L P G I VTEENK EY+RLV + +++ + +Q +F EGF E++P+ + F+ KELE+L+CG+ EIDL D + NT+Y YT + QI WFWQ ++ D E R R +QFVTGT ++P+GGF+ L G NGP KF I + N LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 472 RRTTTYIDPRTGKSSLENGPQITYVRDFKAKVQYFRFWCQQL-----ALPQHIKINVTRKTLFEDSFQQIMSFSAQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSQPFYKRILNKPLGLGDLESIDPEFYNSLIWIKENNIEECGLEMFFSVDKDILGEITTHELKPEGGDIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVLLCGMQEIDLADWQRNTIYRHYTRGNKQIVWFWQLVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIE-KTGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 872
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Match: nedd4a (NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;Acc:ZDB-GENE-051005-2]) HSP 1 Score: 311.997 bits (798), Expect = 2.851e-93 Identity = 177/389 (45.50%), Postives = 245/389 (62.98%), Query Frame = 3
Query: 11049 PKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRL-HRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYV-NNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQ-TETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGY 12200
P D+ K +F+ K+ + D +R+E +++ RA + EDSY R+ K A+ K R I+F E+G D GG+ REW+ +S E+FNP Y LF+ S D T INP S + N HLSYF F+GR A+F KLL +F R FYK +L I D+E D +++ L ++L+ND + LD F I FGQ T +L PNGS+ILVT ENK EY+ LV Q + +++Q+++F+EGF E+IP +LI IF+E ELELL+CGL ++D+ND + +T+Y N Y P IQWFW+A+ D E R R +QFVTGTS+VP+ GF++L G NGP F I + S S LP AHTCFN +DLP YE+FD L + L AI E ++G+
Sbjct: 110 PYSRDYKQKYDFFRKKLKK-PADIPNRFE---MKLHRASILEDSYRRIVAVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPISGLCNEEHLSYFTFIGRVAGMAVFHGKLLDGFFIRPFYKMMLQKPITLNDMESVDSEYYNSLMWILENDPAD--LDLRFCIDEEMFGQQTHLVELKPNGSEILVTNENKKEYIELVIQWRFVERVQKQINAFMEGFSELIPMDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNSYCANHPVIQWFWKAVLLMDGEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEMWSTSEK-LPRAHTCFNRLDLPPYESFDDLREKLNIAI-ENAQGF 490
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Match: wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-000607-82]) HSP 1 Score: 279.256 bits (713), Expect = 9.150e-78 Identity = 134/310 (43.23%), Postives = 201/310 (64.84%), Query Frame = 3
Query: 11277 REWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
REW+ +SHE+ NP Y LF+ + INPAS +N +HL+YF+F+GRFIA A++ K + F+ FYK +L + DLE D +F+ L +L N++ L+ FS+ + G+ + DL P+GS ILVTEENK EY+ L+ + + + + +Q +FL+GF E++P + F+E+ELE+++CG+ EIDL D + +T+Y Y +S QI WFWQ ++ D E R R +QFVTGT ++P+GGF++L G NGP KF I T LP +HTCFN +DLP Y+++++L + LL AI E +EG+G
Sbjct: 623 REWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLAYFRFIGRFIAMALYHGKFIDTGFSLPFYKRMLNKKLTIKDLESIDPEFYNSLIWLRDNNIEECGLEMFFSVDMELLGKVSSHDLKPDGSDILVTEENKEEYIGLMTEWRFSRGVEEQTKAFLDGFSEVVPLQWLLYFDERELEVMLCGMQEIDLADWQRSTIYRHYNRSSKQISWFWQVVKEMDNEKRLRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIEKVGKET-WLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEE-TEGFG 930
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Match: smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:563633]) HSP 1 Score: 249.595 bits (636), Expect = 3.560e-69 Identity = 140/358 (39.11%), Postives = 203/358 (56.70%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQ--YENFDKLHKCLLTAIRECSEGYGMI 12209
++VSR +FE+SY ++ + + K+R +KF EEG D GG+ REW +SHE+ NP Y LF+ S D + T INP S +N HLSYF FVGR + A+F + FT FYK +LG I D+E D + + L ++L N + + T Q R + G ++ + YV + I Q + +GF E+IP+ L+ F+EKELEL+I GL +ID++D K NT TP S ++WFW+A+ESFD+E RAR +QFVTG+S+VPL GF L+G GP F I L T++LP AHTCFN ID+ + YE + ++ ++ C++ YG +
Sbjct: 404 LEVSRTEIFEESYRQVMKMRPRDLKKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAINPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLADMETVDPELHKSLVWILNNSDVNILHEHTVHSCWGGRVQFCFR-TVNKGVPAMIRLAPRRLYVSW----RFLRGIEAQFLALQKGFNELIPQPLLKSFDEKELELIIGGLGKIDVSDWKSNTRLKHCTPESSVVRWFWRAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHLIDAKTDNLPKAHTCFNRIDITRNPYETNNNIYDRVIC----CADFYGGL 752
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Match: AREL1 (apoptosis resistant E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:20363]) HSP 1 Score: 215.698 bits (548), Expect = 5.371e-59 Identity = 129/373 (34.58%), Postives = 204/373 (54.69%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASY-VNNNHLSYFKFVGRFIAKAIFDNKLLTCY-------FTRAFYKHILGISIR--YTDLEKEDYQFFRG-LEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLE-----GFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY--NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 12209
++VSR L + S + ++ +W + F + F NEE D GG REW+ M +F P LF D ++P + ++ L ++ GR + K +F+ L Y FTR+F ++G+ +R Y E +D +F++ + F+L NDVS M L + + + ++ DL+P GS I VT NK Y+ ++ Q ++ +R ++ FL+ G E++P+NL++IF+E ELE+L+CG +I ++D K N V + I WFW + SF QE+ AR +QF TG+S +P GGF+ L FQI ++S LP AHTCFN + LP Y++ ++LHK L AI E SEG+G++
Sbjct: 246 LRVSRQCLLDSSVKAVRNFSSNDWFRSFEVTFENEEALDRGGPRREWFELMCKALFEPANKLFTHFVDDNQALVHPNPHRPHHMRLRLYELAGRLVGKCLFETSLGDTYRQLVRARFTRSFLAQLIGLHVRFLYFYFETDDPEFYKTKVSFVLNNDVSEMDLTLSEEVYDEQGKLSQLVDLVPGGSGIGVTNANKLAYLNVLAQYRLATQVRTEVEHFLKVLSIKGLNEMVPENLLAIFDENELEMLMCGTEDISVDDFKANAVLVGASWHFRKKVILWFWAVVSSFTQEELARLLQFSTGSSLLPTGGFAALS-----PPFQI-VSSPKHGTLPTAHTCFNQLCLPTYDSCEELHKMLRYAITEGSEGFGLL 612
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005015.1 (pep scaffold:Pmarinus_7.0:GL486731:734:6293:1 gene:ENSPMAG00000004557.1 transcript:ENSPMAT00000005015.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 164.081 bits (414), Expect = 2.732e-46 Identity = 79/166 (47.59%), Postives = 116/166 (69.88%), Query Frame = 3
Query: 11706 VRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGY 12200
V LV Q + +++Q+++F+EGF E+IP +LI IF+E ELELL+CGL ++D+N+ + +TVY N Y P +QWFW+A+ D E R R +QFVTGTS+VP+ GF++L G NGP F + + + LP AHTCFN +DLP YE+F++L + L AI E ++G+
Sbjct: 1 VSLVIQWRFVSRVQKQMNAFMEGFCELIPMDLIKIFDENELELLVCGLGDVDVNNWRQHTVYKNGYCANHPVVQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTVEMWG-APEKLPRAHTCFNRLDLPPYESFEELRERLNMAI-ENAQGF 164
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Match: TOM1 (E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase [Source:SGD;Acc:S000002865]) HSP 1 Score: 374.4 bits (960), Expect = 6.634e-104 Identity = 205/510 (40.20%), Postives = 310/510 (60.78%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPD-AVIVFQSLVESFLLC--HSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQ-RFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+W LS+ L +KS+ + + + L+ES ++ SD S+ K +++ + D FS+ N F F + HKK+LN ++R + L F+ L+K PK+LDFD KR +F AK L++D R + + V R ++F DSY L KT +E K + I F E G D GG+ REWY +S ++FNP+YALF P D T+ N S +N HLS+FKF+G I KAI D L C+F+R YK+ILG + D+E D +++ L ++L+ND++ + +++TFS++ ++G+ + +LI G I+VTE NK +YV+ V + K+ ++++Q+ +FL GFY +I K+LI+IF+E+ELELLI GLP+ID++D K NT Y YT ++ +FW+A+ SFD E+RA+ +QFVTGTSKVPL GF +L G+NG KF I S+ LP++HTCFN ++LP YE+++ L LL AI E EG+G+
Sbjct: 2785 VWSSLSECLSQFEKSQAINTSATILLPLIESLMVVCRRSDLSQNRNTAVKYEDAKLLD--------------------FSKTRVENLFFPFTDAHKKLLNQMIR--SNPKLMSGPFALLVKNPKVLDFDNKRYFFNAK---LKSDNQERPKLP-ITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDI-IEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFGL 3267
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Match: RSP5 (NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome [Source:SGD;Acc:S000000927]) HSP 1 Score: 311.997 bits (798), Expect = 6.100e-90 Identity = 160/392 (40.82%), Postives = 239/392 (60.97%), Query Frame = 3
Query: 11043 KYPKLLDFDIK--RRYFQAKVAQLRNDGDSRY--EEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
+ P LD ++ +R F+ KV R+ R + ++V R +FED+Y + R+T E+ K+R IKF EEG D GG+ RE++ +SHE+FNP Y LF+ S D T INP S +N HL+YFKF+GR + +F + L +F A YK +L + D+E D + + L ++L+N + + LD TFS FG+ T DL P+G I VT+ NK EYV L Q ++ +++Q +F++GF E+IP++L+++F+E+ELELLI G+ EID+ D K +T Y Y + IQWFW+ + +D E RAR +QF TGTS++P+ GF L+G +GP +F I A LP +HTCFN +DLPQY ++D + + L A+ E G+G
Sbjct: 419 RLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGV-LDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAG-EVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETI-GFG 807
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Match: HUL5 (Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock [Source:SGD;Acc:S000003109]) HSP 1 Score: 157.918 bits (398), Expect = 7.048e-39 Identity = 116/354 (32.77%), Postives = 172/354 (48.59%), Query Frame = 3
Query: 11151 VSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQ----DGGGLLREWYLKMSHEIF-NPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHIL----GISIRYTDLEKEDYQFFRGLEFLLKNDVSTM-FLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPE-IDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAI 12179
+SR + ED++ + E +K + FINE G+ DGGG+ +E+ +S E F +P + LF+ + Y + P+ + L Y F+G+ + K ++++ L+ F F K +L G ++DL D + L LL + LD TF I E + DLIPNGS+ VT++N YV V K+ + + +F G II + + +FN EL++LI G + IDL+DLK NT Y Y I FW+ L F E++ F++FVT + PL GF L+ KF I A LP A TC N + LP Y N L + LL AI
Sbjct: 558 ISRDNVLEDAFNAFN-SIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRTND---RYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLSSFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEIDEPE-SSAKVVDLIPNGSKTYVTKDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDNIDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKALD-----PKFGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAI 901
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Match: HUL4 (Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex [Source:SGD;Acc:S000003797]) HSP 1 Score: 139.813 bits (351), Expect = 2.519e-33 Identity = 107/348 (30.75%), Postives = 171/348 (49.14%), Query Frame = 3
Query: 11232 IKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVT--YTINPASY----VNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLK------NDVSTMFLDQTFSIQISEFGQTE------TRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPK-NLISIFNEKELELLICGLPE---IDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQIS-LASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
I+F+NE G D GGL +EW+ ++ +FNP LF + + I+P ++ N+ L + G + AIF++ +L F +A YK + + + D + + R L +L DV ++ + T+ ++ T +L NG + +T+ NK E+V + + +I Q + F+ GF + + N I +FN +ELE L+CG E D L+ T Y ++ S + WFW+ +ES+D + + +QFVT + ++P G S + F+IS L S ++ LP AHTCFN I L Y + KL LL AI E SEGYG
Sbjct: 551 IEFVNEPGIDAGGLRKEWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGLAIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNLIKMLNYTEDNFEDVFSLTFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIEPQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSDDSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIP-------FKISLLGSHDSDDLPLAHTCFNEICLWNYSSKKKLELKLLWAINE-SEGYG 890
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Match: UFD4 (Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000001493]) HSP 1 Score: 98.2117 bits (243), Expect = 2.268e-20 Identity = 81/311 (26.05%), Postives = 139/311 (44.69%), Query Frame = 3
Query: 11364 INPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTD------------LEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGF-----YE----IIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNK------YTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISL-----ASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGY 12200
+NP S N + F ++G F+A+++ DN++L F++ F++ + +S +E D + L++++ N M L ++ S+ + G + +LIP G + N EY+ V + + I +QL +F+EGF YE + P L+ IF E D + T+Y YT S I F + +F + +R F+QF+TG+ K+P+GGF L KF + L + +LP+ TC N + LP+Y + D + L AI E + +
Sbjct: 1191 LNPFSN-NEKVIELFGYLGTFVARSLLDNRILDFRFSKVFFELLHRMSTPNVTTVPSDVETCLLMIELVDPLLAKSLKYIVANKDDNMTL-ESLSLTFTVPGNDDI-ELIPGGCNKSLNSSNVEEYIHGVIDQILGKGIEKQLKAFIEGFSKVFSYERMLILFPDELVDIFGRVE-------------EDWSMATLYTNLNAEHGYTMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLN-----PKFTVVLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAGAF 1480
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Match: EDO34473 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP65]) HSP 1 Score: 340.502 bits (872), Expect = 4.265e-104 Identity = 200/424 (47.17%), Postives = 253/424 (59.67%), Query Frame = 3
Query: 10944 FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCI--KFINEEGQDGGGLLREWYLKMSHEIFNPNY-ALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
FL+F E H+ VLN ILR+ T L F+ L+ + ++LDFD+KRR+F+ ++ Q +D R E+ + + R +FEDSY LHR++AEEWK R I + I LL W H F ++ + KVS + RF+ + L + F KH L ++R LE D L K + +F T + + EFG +E RDLIPNG I VTEENK EYV+LVCQ KMTGAIR+Q+ SFLEGFYEIIPK LISIFNE+ELELLI GLP IDL+DLK + Y+KY S QIQWFW+AL SFDQ RA+F+QFVTGTSKVPL GF+ LEGMNG KFQI +T+ LP+AHTCFN +DLP YE +DKL L A EC EG+G+
Sbjct: 23 FLKFAETHRTVLNQILRQSTV-HLSNGPFAVLVDHTRVLDFDVKRRFFRQELEQ--SDEGIRREDLTIPIRRDHVFEDSYRELHRRSAEEWKARLYIVNRLI-------FPLLTIWMSDNIHVQFEYSFTCIIKVS---------------------MELSARFLC----ERGLFSTSLNILFVKHTLN-TVRPQKLELGDT--------LQKVALYILF---TIARLVREFGLSEIRDLIPNGRNIPVTEENKREYVKLVCQMKMTGAIRKQIDSFLEGFYEIIPKRLISIFNEQELELLISGLPNIDLDDLKSHIEYHKYNENSLQIQWFWRALRSFDQAARAKFLQFVTGTSKVPLQGFAALEGMNGFQKFQIHRDDRNTDRLPSAHTCFNQLDLPAYETYDKLRTMLKKACDECPEGFGL 399
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Match: EDO49327 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD5]) HSP 1 Score: 307.76 bits (787), Expect = 2.877e-93 Identity = 152/354 (42.94%), Postives = 227/354 (64.12%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
+ VSR LFE+S+ ++ R + ++R I F EEG D GG+ REW+ +SHE+ NP Y LF+ + + + IN AS VN +HL YFKF+GRFIA A++ K + FT FYK +L + DLE D +F+ L ++ +N++ L+ F++ + G+ + DL P G+ I VTEENK EY+ L+ + ++ I +Q +FLEG +E++P I F+E+ELEL++CG+ EID+ D + NTVY YT S Q+ WFWQA++++D E R R +QFVTGT ++P+GGF++L G NGP KF I T LP +HTCFN +DLP Y+++++L + L AI E +EG+
Sbjct: 11 ITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSLQINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVTEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGDNGPQKFCIEKVGKET-WLPRSHTCFNRLDLPPYKSYEQLVEKLTFAIEE-TEGFA 362
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Match: EDO46071 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR93]) HSP 1 Score: 305.064 bits (780), Expect = 1.398e-85 Identity = 175/390 (44.87%), Postives = 235/390 (60.26%), Query Frame = 3
Query: 11049 PKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYG--RLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYV-NNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNH-LPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGY 12200
P D+ K YF++K+ + N ++YE + + R L EDSY +L E K R I F E G D GGL REW+ +S E+FNP Y LF+ S D T INP S + N HLSYFKF+GR A++ KLL +F R FYK +LG I D+E D +++ L ++L+ND LD F + FG +DL PNGSQ VT ENK EY+ LV + + + Q+ +F+EGF ++IP NLI IF+E+ELELL+CGL EID D + N+ Y +Y IQWFW+A+ SFD E RAR +QFVTGTS+VP+ GFS+L G NGP +F I T H LP AHTCFN +DLP+Y ++ +L + L AI E ++G+
Sbjct: 605 PYSRDYKRKCDYFRSKLKRPSN-VPNKYE---IHIRRRNLMEDSYRAVQLSIVKPEILKSRLWIVFDGETGLDYGGLQREWFYLLSKEVFNPYYGLFEYSASDNYTLQINPNSGLCNEEHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLGRPITLIDMESVDSEYYNSLNWILENDPED--LDLHFCVDEELFGILSVKDLKPNGSQTNVTNENKREYINLVIKWRFVSRVEDQMRAFMEGFCDLIPHNLIQIFDERELELLMCGLGEIDTVDWRKNSNYRGEYHDKHIVIQWFWKAVNSFDIETRARLLQFVTGTSRVPMNGFSELYGSNGPQRFTIE--PWGTPHSLPRAHTCFNRLDLPRYRSYYELRERLRIAI-ENTQGF 985
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Match: EDO49328 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD7]) HSP 1 Score: 279.256 bits (713), Expect = 9.586e-80 Identity = 144/352 (40.91%), Postives = 215/352 (61.08%), Query Frame = 3
Query: 11079 RYFQAKVAQLRN--DGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFW-QALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSI 12122
R F+ KV Q R+ ++ + VSR LFE+S+ ++ R + ++R I F EEG D GG+ REW+ +SHE+ NP Y LF+ + + + IN AS VN +HL YFKF+GRFIA A++ K + FT FYK +L + DLE D +F+ L ++ +N++ L+ F++ + G+ + DL P G+ I V EENK EY+ L+ + ++ I +Q +FLEG +E++P I F+E+ELEL++CG+ EID+ D + NTVY YT S Q+ WFW QA++++D E R R +QFVTGT ++P+GGF++L G NGP KF I T LP +HT + I
Sbjct: 308 RSFRWKVGQFRHLCQANTLPSHIKITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSLQINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVIEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGSNGPQKFCIEKVGKET-WLPRSHTWWVHI 658
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Match: EDO49383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG94]) HSP 1 Score: 255.758 bits (652), Expect = 1.751e-75 Identity = 135/328 (41.16%), Postives = 197/328 (60.06%), Query Frame = 3
Query: 11217 KQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPAS-YVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVY-NKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECS 12191
+ R I+F EEG D GG RE++ +S +IFNP Y LF+ S D T ++P S YV+N+H +F+F GR + I LL +FTR FYK +L +D+E D F + + ++++ND+ + LD TFS+ FGQ R+LIP G I VTE+NK +YV + + ++ + +Q+ S + GF E+I L++IF+ +ELEL+I G +ID+ D + NT Y + Y ++WFW+A+ SFD E R R +QFVTGTS +P GF+ L G GP KF I T LP AHTCFN +D+P Y ++D+L + L A+ E
Sbjct: 10 RNRLEIQFAGEEGLDYGGPAREFFFLISRQIFNPYYGLFEYSANDTYTVQVSPVSLYVDNSH-EWFRFCGRIVGLVIIHQHLLDAFFTRTFYKALLRSLCDLSDVEALDALFHQSMTWVIENDIEDV-LDLTFSVSEEIFGQVTERELIPGGKDIAVTEQNKGDYVAEMVKWRVERGVSEQMESIVRGFNEVIDPALVNIFDARELELVISGTADIDIKDWRRNTEYRSGYHDNHKVVKWFWKAVSSFDNEQRLRLLQFVTGTSSIPYEGFAALRGSTGPRKFSIERWGDYTK-LPRAHTCFNRLDMPIYRSYDELLEKLTYAVEETG 334
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Match: huwe1 (HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101170019]) HSP 1 Score: 589.726 bits (1519), Expect = 3.296e-168 Identity = 294/522 (56.32%), Postives = 367/522 (70.31%), Query Frame = 3
Query: 10692 IWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHS----------DCSEIHEEHNKSDNSNVSD-PLSHLNTSLVVDDTFMNSPAFSENGKHNP-----FLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSP-DGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGM 12206
+W +L + L +++S + AV+V Q VE+F L H+ D E H K + +S PL+ S +D F P+ + P FL F E H+ VLN ILR+ T D F+ L+ Y ++LDFD+KR+YF+ ++ +L D R E+ V V R +FEDSY LHRK+ E+ K R I F EEGQD GGLLREWY+ +S E+FNP YALF+ SP D VTYTINP+S+ N NHLSYFKFVGR +AKA++DN+LL CYFTR+FYKHILG S+RYTD+E EDY FF+GL +LL+NDVST+ + TFS ++ EFG E RDL PNG+ ILVTEENK EYV LVCQ KMTGAIR+QL +FLEGFYEIIPK LISIF E+ELELLI GLP ID++DLK NT Y+KY +S QIQWFW+AL SFDQ DRA+F+QFVTGTSKVPL GF+ LEGMNG KFQI ST+ LP+AHTCFN +DLP YE+++KL LL AI+ECSEG+G+
Sbjct: 3605 LWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLSHIKDEPPPLSPAPLTPATPS-SLDPFFSREPSSMHISSNLPPDTQKFLRFAETHRTVLNQILRQSTTHLAD-GPFAVLVDYIRILDFDVKRKYFRQELERL--DEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEDMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRVVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYPFFQGLVYLLENDVSTLGYELTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMKMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKANTEYHKYQSSSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESYEKLRHMLLLAIQECSEGFGL 4122 HSP 2 Score: 380.563 bits (976), Expect = 8.875e-105 Identity = 251/762 (32.94%), Postives = 394/762 (51.71%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGH--WTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECC---VTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQ-----PESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKC---LSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNM---TGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKI------------------CLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKA 2204
MK+DR K+ +C L +L A ++ L+ E++ I W+ GKCEL W D+LDR D IL + V + W L D+P N L ++A+L FT LIE+S R+LY S EH+ L + +M ++ ++L L Y FSKRSNY +RL S R +L RL +AE+WGGK++GFGLAECC + P SA T+ +EF + +S ++ S+ TL +H++ ESPSE+ME+L YN+ Q LF +RL F ++R + R+ A+SIL Y+N +++ L ++EELVDVL++ D++ + IKA +LRTLT+I+H + P L N+ TG SYHG P ++R I ++ +PY + TA+ SFLYHLA + LV G+++++ ++I + E D I+F TRAVRV D I L+ ++ S + + RL+ E+ + ++SE S+ QR +KSMLN +K+ I D +S+ ++ MDG LP L+ I N + P L L + EV+T FV+ PS +S++Q+ LT +L L + +P R+ L +LP + SAL +N RG++ + + ++ K L ++ AMR +R + + + ++ + +A++EL R + K +I K + + N+G+ AP KP SIQKA
Sbjct: 1 MKVDRSKLKKTPTEAPADCRILIDKLKACSDDQLLVELQHIKTWNIGKCELYHWVDLLDRFDGILCDAGQTVENMSWLLVCDRPENGQLKALLLAVLNFTALLIEYSFSRHLYSSIEHLTNLLASCDMQVVLSVLNLLYVFSKRSNYITRLGSDKRTPLLARLQHLAESWGGKENGFGLAECCRDLPLQKYPPSATTLHFEFYAEPASEVKLDRKSSSN----------------TLHYIHIEQLDKISESPSEIMESLTAMYNIPK-DKQTLLFTHIRLAHGFSNHKKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLVDIKAASLRTLTSIVHLERT----PKLSNIIDCTGTASYHGFLPVLVRNCIQAMIDPVIEPYP----HQFATALFSFLYHLASYDAGGEA----LVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLIT---NLDMAAFQSHSGLSIFICRLEHEVDLSRKECPFVIKPKIQRPSAAVESEDMDTDMDSIQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISN-AEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLHSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASN-----------------LGSAVDELMR-HQPTLKTDATTAIIKLLEEICNLGR---APEYICQKP-SIQKA 707 HSP 3 Score: 66.6254 bits (161), Expect = 7.640e-10 Identity = 81/316 (25.63%), Postives = 122/316 (38.61%), Query Frame = 3
Query: 4875 KHITQLWLIIDQYERTAFALRIRKDV-IYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIML----LPSKSK------------------------DQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLI--SIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSA--IPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIEL 5723
K IT + LIID YE+ A + + R+ + Y W W DDR G W S +N+ I+ ++R G+ + Y F ++ Q NEE+ RP+ML +P SK D + + + + V D V L+D+M+ LI + I D L A L +RL + A A KS I L G+T LVT L R E P L+HT+ ++ V+ GA S+++ Y + P R+ E + S I +
Sbjct: 1571 KWITPVLLIIDFYEKMAVSSKRREQMNKYLQPNGNNWRWFDDRSGRWCSYSASNNSTIDSAWRAGE-SSVRFTAGRRRYTVLFNTMVQVNEETGNRRPVMLTVQRVPRISKPPKTGNVPDSERSEGDKMEIKSKEDSSQLKETTSGPSSTVESVWSQGTDIVVKGLTDDMITVLIRACVSMISVPVDPDTLHATLRLCLRLTRNHHYAM-MFAELKSTRMILGLTQGSGFNGFT-PLVTL---LFRHIIEDPATLRHTMEKVVRSAVTSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPECFIETASNCIRI 1880 HSP 4 Score: 57.3806 bits (137), Expect = 4.824e-7 Identity = 27/73 (36.99%), Postives = 48/73 (65.75%), Query Frame = 3
Query: 2562 VYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWA 2780
+ LMDYI NV KF+E +S+ + Q+ +++GL ++ IL +P+LP DFP+S ACQ++ + + +L+ +
Sbjct: 761 IPLMDYILNVMKFVESILSNNTTDDHC-QEFVNQKGLLPLVSILGLPNLPIDFPTSAACQAVAGVCKSILTLS 832
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Match: smurf2 (E3 ubiquitin-protein ligase SMURF2 [Source:NCBI gene;Acc:101160323]) HSP 1 Score: 318.161 bits (814), Expect = 3.882e-92 Identity = 162/348 (46.55%), Postives = 219/348 (62.93%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRE 12185
++VSR +FE+SY ++ + ++ +R +KF EEG D GG+ REW +SHE+ NP Y LF+ S D + T INP S VN HLSYF FVGR + A+F + FT FYK +LG I D+E D L ++L ND+ T LD TF ++ + +G+ +L PNG I VT+E K EYVRL + I Q + +GF E+IP++L+ F+EKELEL++CGL +ID++D K NT TP S ++WFW+A+E FD+E RAR +QFVTG+S+VPL GF L+G GP F I STN+LP AHTCFN ID+P YE +DKL+ LLTAI E
Sbjct: 357 IEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDI-TGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTQETKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIHDWKGNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYETYDKLYDKLLTAIEE 703
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Match: smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101167123]) HSP 1 Score: 317.39 bits (812), Expect = 7.155e-92 Identity = 157/348 (45.11%), Postives = 225/348 (64.66%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRE 12185
++VSR +FE+SY ++ + ++ K+R +KF EEG D GG+ REW + HE+ NP Y LF+ S D + T INP S +N +HLSYF FVGR + A+F + FT FYK +LG I+ DLE D + + L ++L+ND++++ LD TF ++ + FG+ +L PNG + VTEENK EYVRL + I Q + +GF E+IP++L+ F+ KELEL+I GL +IDL D K NT T S ++WFWQA+E+F +E R R +QFVTG+++VPL GF L+G GP F I L +T++LP AHTCFN ID+P YE+++KL++ LLTA+ E
Sbjct: 353 IEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRVMGLAVFHGHYINGSFTLPFYKQLLGKPIQLNDLETTDPELHKSLVWILENDITSV-LDHTFCVEHNAFGKLSQHELKPNGRNVAVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFGELIPQHLLKPFDHKELELIIGGLGKIDLADWKTNTRLKHCTSESNVVRWFWQAVEAFSEERRGRLLQFVTGSTRVPLQGFKALQGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEE 699
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Match: itcha (E3 ubiquitin-protein ligase Itchy [Source:NCBI gene;Acc:101167949]) HSP 1 Score: 320.087 bits (819), Expect = 7.862e-92 Identity = 168/409 (41.08%), Postives = 243/409 (59.41%), Query Frame = 3
Query: 10995 RRTACALD--QSNFSALLKYPKLL---DFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFK-VSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
RRT +D S+L P++ DF K +YF+ QL S + + VSR LFEDS+ ++ ++ ++R I F EEG D GG+ REW+ +SHE+ NP Y LF+ D INPASY+N +HL YFKF+GRFIA A+F K + F+ FYK IL + DLE D +F+ L ++ N++ L+ FS+ G+ T +L P+G I VTE+NK EY+RLV + +++ + +Q +F EGF E++P+ + F+ KELE+++CG+ EIDL D + NT+Y Y S QI WFWQ ++ D E R R +QFV GT ++P+GGF+ L G NGP KF I N LP +HTCFN +DLP Y+ +++L + L+ AI E +EG+G
Sbjct: 393 RRTTTYIDPRTGKSSSLENGPQITYVRDFKAKVQYFRFWCQQL-----SMPQHIKITVSRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLTLKDLESIDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEDNKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLADWQRNTIYRHYARNSKQIIWFWQFVKEMDNEKRMRLLQFVAGTCRLPMGGFADLMGSNGPQKFCIEKVG-KENWLPRSHTCFNRLDLPPYKTYEQLKEKLMFAIEE-TEGFG 794
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Match: smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101167123]) HSP 1 Score: 317.775 bits (813), Expect = 9.820e-92 Identity = 157/348 (45.11%), Postives = 225/348 (64.66%), Query Frame = 3
Query: 11145 VQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGV-TYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRE 12185
++VSR +FE+SY ++ + ++ K+R +KF EEG D GG+ REW + HE+ NP Y LF+ S D + T INP S +N +HLSYF FVGR + A+F + FT FYK +LG I+ DLE D + + L ++L+ND++++ LD TF ++ + FG+ +L PNG + VTEENK EYVRL + I Q + +GF E+IP++L+ F+ KELEL+I GL +IDL D K NT T S ++WFWQA+E+F +E R R +QFVTG+++VPL GF L+G GP F I L +T++LP AHTCFN ID+P YE+++KL++ LLTA+ E
Sbjct: 375 IEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRVMGLAVFHGHYINGSFTLPFYKQLLGKPIQLNDLETTDPELHKSLVWILENDITSV-LDHTFCVEHNAFGKLSQHELKPNGRNVAVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFGELIPQHLLKPFDHKELELIIGGLGKIDLADWKTNTRLKHCTSESNVVRWFWQAVEAFSEERRGRLLQFVTGSTRVPLQGFKALQGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEE 721
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Match: SMESG000059703.1 (SMESG000059703.1) HSP 1 Score: 7947.81 bits (20621), Expect = 0.000e+0 Identity = 4052/4065 (99.68%), Postives = 4055/4065 (99.75%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSGVYSSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSXXXXXXXXXXXGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANS--SVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPXXXXXXXXXXXXXXXNNQSQESLMNIIAFLTSYIFDRDXXXXXXXXXXXXXIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRXXXXXXXXXXXXXXXXXVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSLTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQNQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSXXXXXXXXXXXXXXXADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTAAVDNMENLEASNADEHPXXXXXXXXXXXXXXXXXXXXSEFRDIDAEYDFDEPQMYDDDNNXXXXXXXXXXXXXXXXXXXXXXXXXXXRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPNYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVXXXXXXXXXXXXFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPVAAEWSIATNDGDNHESSTPTASTGNETPLSITKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 12209
MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPS VY SDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANS SVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPS TSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQ QFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLEL+NKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDT AVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHP+YVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSI DQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGAT AAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKP AAEWSIATNDGDNHESSTPTASTGNETPLSI+KEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI
Sbjct: 1 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTNQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSAVYPSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSERSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSPTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQIQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLELINKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTVAVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPSYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSINDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGATAAAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPAAAEWSIATNDGDNHESSTPTASTGNETPLSISKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 4065
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Match: SMESG000059703.1 (SMESG000059703.1) HSP 1 Score: 7942.8 bits (20608), Expect = 0.000e+0 Identity = 4051/4066 (99.63%), Postives = 4055/4066 (99.73%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNST---NQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSGVYSSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSXXXXXXXXXXXGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPXXXXXXXXXXXXXXXNNQSQESLMNIIAFLTSYIFDRDXXXXXXXXXXXXXIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRXXXXXXXXXXXXXXXXXVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSLTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQNQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSXXXXXXXXXXXXXXXADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTAAVDNMENLEASNADEHPXXXXXXXXXXXXXXXXXXXXSEFRDIDAEYDFDEPQMYDDDNNXXXXXXXXXXXXXXXXXXXXXXXXXXXRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPNYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVXXXXXXXXXXXXFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPVAAEWSIATNDGDNHESSTPTASTGNETPLSITKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 12209
MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNST +QGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPS VY SDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPS TSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQ QFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLEL+NKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDT AVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHP+YVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSI DQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGAT AAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKP AAEWSIATNDGDNHESSTPTASTGNETPLSI+KEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI
Sbjct: 1 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTSTSDQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSAVYPSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSPTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQIQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLELINKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTVAVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPSYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSINDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGATAAAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPAAAEWSIATNDGDNHESSTPTASTGNETPLSISKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 4066
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Match: SMESG000059703.1 (SMESG000059703.1) HSP 1 Score: 7937.79 bits (20595), Expect = 0.000e+0 Identity = 4051/4068 (99.58%), Postives = 4055/4068 (99.68%), Query Frame = 3
Query: 21 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNST---NQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDXXXXXXXXXXXXXXYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSGVYSSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSXXXXXXXXXXXGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANS--SVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPXXXXXXXXXXXXXXXNNQSQESLMNIIAFLTSYIFDRDXXXXXXXXXXXXXIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRXXXXXXXXXXXXXXXXXVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSLTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQNQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSXXXXXXXXXXXXXXXADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTAAVDNMENLEASNADEHPXXXXXXXXXXXXXXXXXXXXSEFRDIDAEYDFDEPQMYDDDNNXXXXXXXXXXXXXXXXXXXXXXXXXXXRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPNYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVXXXXXXXXXXXXFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSITDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLXXXXXXXXXXXXXNDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFXXXXXXXXXILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPVAAEWSIATNDGDNHESSTPTASTGNETPLSITKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMIXXXXXXXXXXXXPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHXXXXXXXXXXRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 12209
MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNST +QGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPS VY SDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANS SVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPS TSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQ QFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLEL+NKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDT AVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHP+YVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSI DQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGAT AAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKP AAEWSIATNDGDNHESSTPTASTGNETPLSI+KEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI
Sbjct: 1 MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCELGLWADVLDRLDNILAEGVTKVGHWTLKLDQPGNEHLIGKIIAILEFTGHLIEHSIYRYLYGSWEHILALFGTSNMDILHALLGLFYNFSKRSNYFSRLDSTNRKIVLERLDSIAENWGGKQHGFGLAECCVTEYMPYSAGTMQYEFMQDVSSLSSAGKEDSNQSSVDVFIKPKCHRRKLTLRNLHLQPESPSELMENLIQKYNLTNTSDQMALFARLRLCVYFGKPEQRKLLIYTRMQALSILCYTNEVEDKCLSWDIVEELVDVLKLPDREFLGIKACALRTLTAIIHCGKLTSCWPVLKNMTGMNSYHGHFPTMMREWITDLVQSKFQPYASTVEQMYTTAILSFLYHLACTNEVNSTSTSDQGNLVGTGLLDSMKEIIMWHTHEPDYISFATRAVRVTDQILLSFQLNGSSTNQKSTIDAIVDRLKFEIKICLDSEIDQMTKKSVALNTQRVGFIKSMLNLIKRLILDNEWSETLKATMDGELPDILRLIFMNYTTKFNPQLCLFSMEVITNFVYNHPSSMSAMQERQLTKDILRTLTEQPLPVNRDFLVTLPTLLSALSINIRGINEIKESKIIDKYIKTLTDSRFVTAMRNKRIREMMTPSENSDTATTNHSHPSTPTSYQMSNAMNELARANAVVFKPIIYESISKAFKTVTNIGQKQPAPLLGNGKPGSIQKALNHHHNNEHSNPITIDDNEIMPLQENVDSQEIASTAGPSAVYPSDSNQAADLLAVMTAVEEADREVEQIMEMSGDEDDGVADNEMTQAIDAEEDRTPSDGCGKDNKTEEMNQFSCDDDAVYLMDYICNVCKFMERFMSDQQPEHSLRQQIYSEEGLKTILDILTIPSLPYDFPSSPACQSMQKIFEYLLSWATANDAILIYQMFFKISKKVGDRLQKLEQPQLYSILLKDYVNRSEEFQDSPALMVATKTYWEENGVCLLHDLVILSTVIVIYDKITDRLSNNLRQDILAELKSGIIDYARLLPLLNWEAMVLMNLLFEDEEACLEDLKRLCINERGTCADNEHNEERCFLTKKSSLSTCQQLFSDNPRSEKIIKRMLQITQKAANDMVELLVTLTKFCCVKPDYSYVVRVRYNRVNGSIPTGKNKMALLNKIAEHCKSLLERESPYTLQAGQDSWPEDPHSELPVEKACQKILSDIKFLHPMKYFCFTTIHRILTGGDLNKKPFLGFFYAFQMNNGLEVFFECANELLDTIRYIQYLDPNANSERSVKIMLESFLITVDRMTQIRRITQEFVDSTDKELRDKFDIDLYQRMIRAYMLEPISKLFSFSSLDIDLLNNQSQESLMNIIAFLTSYIFDRDTKKSESEETPEEPIVFNDDHEQLINQHISFIMELGFSRENARRALERNDYDPTQAVSSLFNSDLGIRTAESSSSTNEPETSSKSVRPNPLNENSREISTIEARLKELEMKNVSIKEMIKESFLINLFNLQKEKGIDIITRIVDILLLTGQINEFLEKILAINGCSFLTSDDIYSNILRTLILNHSGKEISGANIEDSPNGLHILTLLYNKIRFQVTAAFKNHDMVTRLVKMTNLLFKSFMAVPHQTETFKTSVLKHITQLWLIIDQYERTAFALRIRKDVIYEYQGCPTWFWIDDRHGIWHPVSTQHNANINRSYRNGQLKAYSKPNSNNEYVFNFVSLTQKNEESQTERPIMLLPSKSKDQRKFENLSEYEHKIISCVPDPLGDEEVVELSDNMLQFLISIKKIDNVYKWDCLQAACYLLIRLCDKYSVASERLAAYKSAIPRLCTIPHVKGYTQQLVTFVMTLLRQQFETPQLLQHTISDILDKNVSKGACVLHKTTSYNDKLSKDLFYFIWDNIPLLVRDFETTMNLMSKKIELNCQTEKQVLKTYSISKSPSPTSKATQPLMELEKKQIAIVRFLVESALSYYIEKNDVWCELFKKLKLNTEYDLKNMIASPNYLLETHTVVTSNLENNFSSNQHSSGTVKHTYKLTGKEFLIYLEELIFSYEQVGAFTINLRLFNRPLSFYLVNIYLQEACALLDSMRSKEPGSNTSPSMSREQIQFQQLVSLQIIEAFLLTVKESYDVIIGEIVRNLKMRSHHKDGDCRNTYIVVIMKLLNDLITRLPVSVSKKLVILMYKYRLPNDLCRLLSVVDCSVAQHKSVLVDILTVVDSLCRPERQMYINKRQRSSKSNLELINKRQRRLNNNADTQATSVIAGTSAATNEITAQEMETDDVFYVDINAWSRNSRSENTDTVAVDNMENLEASNADEHPVDVDDDEEMDEYDEVDGVMMSEFRDIDAEYDFDEPQMYDDDNNTESETDYEEIEEDEGSAGSQDGDSQGDRFDEDIRETENSSHSNGRQEQSRRIFQILNHNYYNISQEGENTETPRHDTETSLRDRLSNVQMMSTYDHHPSYVLNESFSLDAPPSQDDTSTFRLADNLYFVTNSREYDISSQPSNYYPNDLAGCSNTPSNNLLRTTNLESSAVSQNPQVPTGSQEDSINGRGWRGLTYAPGARYMRLEARTLRQNTIRNILTLNRGSFMDRAPNTRSPSNRFVDADLSLDSLEADFEMQEILARALSNIPSGWNFLINPTASAPQNTINNFTFNDISMITNFRRWIVEARTLYGREITDLVMLARARVYQSVYHSVSSKLTEASVDEKEVVDSKTNMVKTANSNPDNVDNERNSINDQTDIGRDVSMEQDLMMQPDIMIESLDEPNESVIGEEMVTPTPSETMESRDEPIDNIEIEQPNEVISRVDQSLGAVGGATAAAARANDEDIDNLSMEQLIAEGIDPTFLEALPQSMRREVIIEHRRANRVRQQLTGIQLPENVDAEIMAQLPAHIQEEILAHYRRENSTSETANAPSLTNPESGSLPLISSSTGVVDSSFLLSLAPSLRREILADMDQSQIANLSSELADEARQVRQETGDAQMRALQANLLSVTGRRRARQLTSGLSDLLSQMNRGRWDQRNIRYVVFSERGQTNRHNNAAVTDPVTEAGEWPNLSAYKPLVSTDGLTNLIALLITSSTSPSVMKMISTLVNKILQSLTCHPQIRAWISCTIVNILLKISLPKENTDSKPKIFSSPSTSESSILCTPIGSGSCTDMSRSSSIQDDTFRQYPNNKSLFFHSFERGFALTDQVFVPSISKIYKHKNKSGIQSLEMPEITIHESVSGSVAKALIDSLSSISTMHTKEFFPTSQSKKVPTNFWFTLAWYMSLKSVSLEDQCPETHLPPNEEYDYFTHLLCLVRLPLMQSNNAMLEKLLSTIHNILKAYYKSFSMKEKPAAAEWSIATNDGDNHESSTPTASTGNETPLSISKEKSETKAVNVSDPHLNRVNVKTISHLVNIICAKECSSQTPLPTDKCRMFSERLLIELSKGNVNTKQDIIRLLTSNIELVLETLTGVLHKVLDEAVMILENECNETCLNEPSGSTASPIKKSNTFTESISSDILTSNLSPSYENAFTSASSRRNQAQVSSGYSRNNSLPHIAFNQLKMESIKPFTANTGDHLNLLDLFNLLRKVDNSILTQFQSPSSIWHLLSQVLDAIQKSKEPDAVIVFQSLVESFLLCHSDCSEIHEEHNKSDNSNVSDPLSHLNTSLVVDDTFMNSPAFSENGKHNPFLEFVEKHKKVLNHILRRRTACALDQSNFSALLKYPKLLDFDIKRRYFQAKVAQLRNDGDSRYEEELVQVSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDGVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYGMI 4068
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Match: SMESG000038772.1 (SMESG000038772.1) HSP 1 Score: 304.679 bits (779), Expect = 1.194e-87 Identity = 153/378 (40.48%), Postives = 241/378 (63.76%), Query Frame = 3
Query: 11079 RYFQAKVAQLRNDGDSRYEEELVQVS--RARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPD-GVTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
R F+ ++ +++ S V+++ R L + S+ ++ + E KQR I+F+ EEG D GG+ +EW+L++SHEI NP Y LF+ + + INP S+VN NHLSYFKF+GRFI A F + + FT FYK IL + D+E D +F++ L F+ +N ++ L+ FS G+ + +LIP G+++ VT+ENK EY++L+ +T + +Q+ +FLEG YE++P +L+ F+E+ELELL+CGL +ID++D + +TVY +Y+P++ Q+ WFW+ + S D E +AR +QFVTGT ++PLGGFS+L+G NG F + T LP +HTCFN +DLP Y+ +++ + L A E +EG+G
Sbjct: 330 RSFRYRLEAFQDECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSLKINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGRQLFCLERVGDET-WLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEE-TEGFG 705
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Match: SMESG000008708.1 (SMESG000008708.1) HSP 1 Score: 300.827 bits (769), Expect = 5.819e-86 Identity = 142/352 (40.34%), Postives = 227/352 (64.49%), Query Frame = 3
Query: 11151 VSRARLFEDSYGRLHRKTAEEWKQRFCIKFINEEGQDGGGLLREWYLKMSHEIFNPNYALFKVSPDG-VTYTINPASYVNNNHLSYFKFVGRFIAKAIFDNKLLTCYFTRAFYKHILGISIRYTDLEKEDYQFFRGLEFLLKNDVSTMFLDQTFSIQISEFGQTETRDLIPNGSQILVTEENKSEYVRLVCQEKMTGAIRQQLHSFLEGFYEIIPKNLISIFNEKELELLICGLPEIDLNDLKINTVYNKYTPASPQIQWFWQALESFDQEDRARFIQFVTGTSKVPLGGFSKLEGMNGPTKFQISLASMSTNHLPAAHTCFNSIDLPQYENFDKLHKCLLTAIRECSEGYG 12203
V R L EDS+ + R ++ ++R I+F +E+G D GG+ REW+ ++SHE+ NP Y LF+ + D + INPAS++N HL YF+FVGRF+A A+F K + FT FYK +LG I D+E D +++ L+F+ N++ + FS + FG+ E ++L NG I VTEENK+EY+ L+ + + + +Q +F++GF EI+P + + +++E+++ELL+CG+ +ID+ D + N++Y YT SPQI+WFW+ + +++ R +QFVTGT ++P+GGF +L G NGP KF I + LP +HTCFN +DLP Y ++++L + L A+ E +EG+G
Sbjct: 394 VRRNMLMEDSFNEIMRVKPKDLQKRLFIQFKDEDGLDYGGISREWFFQLSHELLNPMYCLFEYASDNNYSLQINPASFINPEHLQYFRFVGRFVAMAMFHRKFIDSGFTIPFYKKMLGKRITLQDIETVDVEYYNSLKFIRDNNIDECDMGLVFSAEYELFGKIEEQELKTNGRDIEVTEENKNEYIDLMVNWRFSRGVVEQTKAFMDGFNEIVPLHWLQVYDERQIELLLCGMQQIDVKDWEANSIYRVYTKDSPQIKWFWEFVGKLPNDEKIRLLQFVTGTCRLPVGGFRELMGSNGPQKFCIEKIGKES-WLPRSHTCFNRLDLPPYASYEQLKEKLTYAVME-TEGFG 743 The following BLAST results are available for this feature:
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase HUWE1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006629 ID=SMED30006629|Name=E3 ubiquitin protein ligase HUWE1|organism=Schmidtea mediterranea sexual|type=transcript|length=12667bpback to top protein sequence of SMED30006629-orf-1 >SMED30006629-orf-1 ID=SMED30006629-orf-1|Name=SMED30006629-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=4064bp MKIDRKKIFNRCGFDQRECYDLAVRLAALGENDLINEIKKINVWHYGKCEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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