SMED30006541
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006541 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 25Note: Hover over icons to view figure legend
Alignments
SMED30006541 aligns in the following genomic locations:
Homology
BLAST of SMED30006541 vs. Planmine SMEST
Match: SMESG000058958.1 (SMESG000058958.1) HSP 1 Score: 393.275 bits (1009), Expect = 5.594e-139 Identity = 201/261 (77.01%), Postives = 218/261 (83.52%), Query Frame = 3 Query: 99 MSVEILYRNEIFTQEVSTDMSVKMLREKLAEIKSLNIADFSLKIDQLLLLEDKPVHKYLNTEFSLTDNGKYIKIEINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDLVVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMM 881 MSVEILYRNEIFTQEVSTDMSVK+L+EKLAEIK LNIADF+LKIDQLLLLEDKPVHKYLNTEFSLTDN KYIK+EINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKK FTIYFQ F+VIV C R++SVTVTKDMTILEFRKECFKKIGEQIN+ L+LF+GESLDYE + +LTLGFYE+ P SV+ L V+GGFIY++ Sbjct: 1 MSVEILYRNEIFTQEVSTDMSVKVLKEKLAEIKLLNIADFALKIDQLLLLEDKPVHKYLNTEFSLTDNQKYIKLEINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYE----------------------------RPDLTLGFYEIGPGSVVELASNVRGGFIYII 233
BLAST of SMED30006541 vs. Planmine SMEST
Match: SMESG000058954.1 (SMESG000058954.1) HSP 1 Score: 374.015 bits (959), Expect = 9.734e-132 Identity = 186/187 (99.47%), Postives = 186/187 (99.47%), Query Frame = 3 Query: 330 DLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDLVVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMMSHE 890 DLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDL VGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMMSHE Sbjct: 23 DLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDLFVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMMSHE 209
BLAST of SMED30006541 vs. Planmine SMEST
Match: SMESG000058958.1 (SMESG000058958.1) HSP 1 Score: 355.525 bits (911), Expect = 2.749e-124 Identity = 182/242 (75.21%), Postives = 199/242 (82.23%), Query Frame = 3 Query: 156 MSVKMLREKLAEIKSLNIADFSLKIDQLLLLEDKPVHKYLNTEFSLTDNGKYIKIEINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRFTIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLDYEKVITLEDLVVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGGFIYMM 881 MSVK+L+EKLAEIK LNIADF+LKIDQLLLLEDKPVHKYLNTEFSLTDN KYIK+EINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKK FTIYFQ F+VIV C R++SVTVTKDMTILEFRKECFKKIGEQIN+ L+LF+GESLDYE + +LTLGFYE+ P SV+ L V+GGFIY++ Sbjct: 1 MSVKVLKEKLAEIKLLNIADFALKIDQLLLLEDKPVHKYLNTEFSLTDNQKYIKLEINDLTFAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKMFTIYFQPFIVIVKCAGRSHSVTVTKDMTILEFRKECFKKIGEQINDLLVLFDGESLDYE----------------------------RPDLTLGFYEIGPGSVVELASNVRGGFIYII 214
BLAST of SMED30006541 vs. Planmine SMEST
Match: SMESG000058973.1 (SMESG000058973.1) HSP 1 Score: 100.523 bits (249), Expect = 1.478e-24 Identity = 79/262 (30.15%), Postives = 137/262 (52.29%), Query Frame = 3 Query: 99 MSVEILYRNEIFTQEVSTDMSVKMLREKLAEIKSLNIADFSLKIDQLLLLEDKPVHKYLNTEFSLTDNGKYIKIEINDLT--FAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRF-TIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLD---YEKVITLEDLVVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGG 866 MS+ + + N +++ V+T M+V L+ +++ +++ DF LK++ L+ E++ V L T+F LT+N + I INDLT F + I +I L+A + SI DLK I +K+ W MK ++ I+ D L N I+ K I + F V V RNY V+ +D TI EF+ +KIG ++ F + FNG+S++ + K L LF ++ V + + L +E+ N+++ ++ + +GG Sbjct: 1 MSINLTFDNFTYSETVTTTMTVSELKLRVSSKIKMDVKDFLLKVNNTLMKEEQLVVSLLFTDFVLTNNTNVLNIRINDLTYDFVMDIPNSEEKIHLKANSNSSILDLKIIISENIKINLKTWKMKISDNILKDIMLLTNCIEVKTIQVIANEEFDVHVINDKRNYKVSTKRDTTITEFKLAVSEKIGLPVDTFYMTFNGQSIENVRHRKFSLKSGLFFKNLFRSNISITKPVVNVVGEQMVLESFEINKNAIIHVIMRTRGG 262
BLAST of SMED30006541 vs. Planmine SMEST
Match: SMESG000058973.1 (SMESG000058973.1) HSP 1 Score: 100.523 bits (249), Expect = 1.614e-24 Identity = 79/262 (30.15%), Postives = 137/262 (52.29%), Query Frame = 3 Query: 99 MSVEILYRNEIFTQEVSTDMSVKMLREKLAEIKSLNIADFSLKIDQLLLLEDKPVHKYLNTEFSLTDNGKYIKIEINDLT--FAIKITEKSREISLEATAEISIGDLKNKIMNKLKLTGAQWSMKKNNEIIDDKDSLLNHIDKKRF-TIYFQTFVVIVNCVDRNYSVTVTKDMTILEFRKECFKKIGEQINEFLILFNGESLD---YEKVITLEDLVVGILFNIDVINIHQTEKVKKDNLTLGFYEVVPNSVLVLVPKVKGG 866 MS+ + + N +++ V+T M+V L+ +++ +++ DF LK++ L+ E++ V L T+F LT+N + I INDLT F + I +I L+A + SI DLK I +K+ W MK ++ I+ D L N I+ K I + F V V RNY V+ +D TI EF+ +KIG ++ F + FNG+S++ + K L LF ++ V + + L +E+ N+++ ++ + +GG Sbjct: 5 MSINLTFDNFTYSETVTTTMTVSELKLRVSSKIKMDVKDFLLKVNNTLMKEEQLVVSLLFTDFVLTNNTNVLNIRINDLTYDFVMDIPNSEEKIHLKANSNSSILDLKIIISENIKINLKTWKMKISDNILKDIMLLTNCIEVKTIQVIANEEFDVHVINDKRNYKVSTKRDTTITEFKLAVSEKIGLPVDTFYMTFNGQSIENVRHRKFSLKSGLFFKNLFRSNISITKPVVNVVGEQMVLESFEINKNAIIHVIMRTRGG 266 The following BLAST results are available for this feature:
BLAST of SMED30006541 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006541 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006541 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006541 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006541 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006541 ID=SMED30006541|Name=SMED30006541|organism=Schmidtea mediterranea sexual|type=transcript|length=984bpback to top protein sequence of SMED30006541-orf-1 >SMED30006541-orf-1 ID=SMED30006541-orf-1|Name=SMED30006541-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=265bp MSVEILYRNEIFTQEVSTDMSVKMLREKLAEIKSLNIADFSLKIDQLLLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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