tRNA-splicing endonuclease subunit Sen2

Overview
NametRNA-splicing endonuclease subunit Sen2
Smed IDSMED30006513
Length (bp)824
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA-splicing endonuclease subunit Sen2 (SMED30006513) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA-splicing endonuclease subunit Sen2 (SMED30006513) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA-splicing endonuclease subunit Sen2 (SMED30006513) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA-splicing endonuclease subunit Sen2 (SMED30006513) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30006513

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006513SMESG000065232.1 SmedASXL_010473SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Match: TSEN2 (tRNA splicing endonuclease subunit 2 [Source:HGNC Symbol;Acc:HGNC:28422])

HSP 1 Score: 74.3294 bits (181), Expect = 1.478e-14
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPS 657
            E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK+ ++    +PS
Sbjct:  236 EYLQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPS 368
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Match: TSEN2 (tRNA splicing endonuclease subunit 2 [Source:HGNC Symbol;Acc:HGNC:28422])

HSP 1 Score: 73.9442 bits (180), Expect = 2.185e-14
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPS 657
            E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK+ ++    +PS
Sbjct:  295 EYLQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPS 427
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Match: TSEN2 (tRNA splicing endonuclease subunit 2 [Source:HGNC Symbol;Acc:HGNC:28422])

HSP 1 Score: 73.9442 bits (180), Expect = 2.185e-14
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPS 657
            E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK+ ++    +PS
Sbjct:  295 EYLQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPS 427
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Match: TSEN2 (tRNA splicing endonuclease subunit 2 [Source:HGNC Symbol;Acc:HGNC:28422])

HSP 1 Score: 70.4774 bits (171), Expect = 3.130e-13
Identity = 42/131 (32.06%), Postives = 70/131 (53.44%), Query Frame = 1 
Query:  283 ETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVV 666
            E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK+ ++    +PS + 
Sbjct:  277 EAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMT 404
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Match: TSEN2 (tRNA splicing endonuclease subunit 2 [Source:HGNC Symbol;Acc:HGNC:28422])

HSP 1 Score: 68.1662 bits (165), Expect = 2.141e-12
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = 1 
Query:  283 ETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSK 624
            E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK
Sbjct:  277 EAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSK 390
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Celegans
Match: tsen-2 (Trna (tRNA) Splicing ENdonuclease subunit related [Source:UniProtKB/TrEMBL;Acc:Q9U218])

HSP 1 Score: 48.521 bits (114), Expect = 2.025e-6
Identity = 28/91 (30.77%), Postives = 46/91 (50.55%), Query Frame = 1 
Query:  265 LTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDC--VTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI 531
            L L+ IET  +   +  IN+     K  +S E IW +   + +N   F  ++  + + + +GW+V SG  FG DYL+Y    E  H+S  +
Sbjct:  113 LVLTPIETALLSIDWKIINV-VKNGKISLSDEEIWTEMRNLYDNPKEFGKVFSVFRFLKMNGWIVTSGHTFGCDYLIYCLGAEFYHSSAGV 202
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Fly
Match: CG31812 (gene:FBgn0051812 transcript:FBtr0290062)

HSP 1 Score: 62.003 bits (149), Expect = 4.565e-11
Identity = 60/208 (28.85%), Postives = 100/208 (48.08%), Query Frame = 1 
Query:   79 GERHPSIDFCKGKIAVLKKQSIYFVDLPGHTEFLIYCGSFGKLMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVEN--IWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVVKCKSIPKRIS 696
            GER+      KG+   L   S+  VD P   + L   G FGK   S    ++ D NE     L L   E+ F+ +  N ++I    T S   + +  ++ +    +  +F+    +Y Y +S  WV++SG++FG D+L+Y  SP   HASF + V+    G+T DH+  + +    RV+   S K ++ ++ +P         PK+IS
Sbjct:   28 GERY------KGRFTGL---SVEIVD-PEQAKSLHDNGCFGKGSKSRGGPASGDENET----LFLGMEESCFLAHYLNVLDI----TNSAGYLMDFCMFVEVAKEHNEKFVENLASYVYLKSKNWVIKSGIKFGGDFLIYKQSPRLYHASFLVIVQ--KPGDT-DHYQSKNLKGVQRVAE-TSDKDVLLLTVQP---------PKKIS 204
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Zebrafish
Match: tsen2 (TSEN2 tRNA splicing endonuclease subunit [Source:ZFIN;Acc:ZDB-GENE-060929-918])

HSP 1 Score: 75.0998 bits (183), Expect = 5.398e-16
Identity = 50/157 (31.85%), Postives = 78/157 (49.68%), Query Frame = 1 
Query:  211 NSSDILSNLDPNENFP--ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI---NVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVV 666
              ++++  ++P   FP  E L LS  E  F+ Y    ++I  YY    +SV  +W     +    F   Y AYHYYRS GWV +SG+++GTD +LY   P   HAS+S+    V      ++L  F+   +   +R +  VSK+ ++     PS V 
Sbjct:    2 QPAELVCRINP---FPMLEYLQLSYEEAFFLVYALGCLSI--YYNGEPLSVAQLW-TMFRSLQPNFCFSYAAYHYYRSKGWVPKSGVKYGTDLMLYRKGPPFYHASYSVVVDTVDASFRRSSLRPFSWRSLATLSRTTGNVSKELMLCFIITPSDVT 152
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Mouse
Match: Tsen2 (tRNA splicing endonuclease subunit 2 [Source:MGI Symbol;Acc:MGI:2141599])

HSP 1 Score: 81.2629 bits (199), Expect = 6.200e-17
Identity = 51/158 (32.28%), Postives = 84/158 (53.16%), Query Frame = 1 
Query:  202 KLMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLN---GNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVV 666
            KL+    ++   +P   F E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++   +   G+    F+ + +   +RVS  VSK+ ++    +PS + 
Sbjct:  272 KLLKRKKLVCRRNPYRIF-EYLQLSLEEAFFLAYALGCLSI--YYEKEPLTIVKLWQ-AFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRPFSWKSLAALSRVSGNVSKELMLCYLIKPSTMT 425
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Match: sp|Q6P7W5|SEN2_MOUSE (tRNA-splicing endonuclease subunit Sen2 OS=Mus musculus OX=10090 GN=Tsen2 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.337e-16
Identity = 51/158 (32.28%), Postives = 84/158 (53.16%), Query Frame = 1 
Query:  202 KLMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLN---GNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVV 666
            KL+    ++   +P   F E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++   +   G+    F+ + +   +RVS  VSK+ ++    +PS + 
Sbjct:  272 KLLKRKKLVCRRNPYRIF-EYLQLSLEEAFFLAYALGCLSI--YYEKEPLTIVKLWQ-AFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRPFSWKSLAALSRVSGNVSKELMLCYLIKPSTMT 425
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Match: sp|Q5M954|SEN2_RAT (tRNA-splicing endonuclease subunit Sen2 OS=Rattus norvegicus OX=10116 GN=Tsen2 PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.532e-15
Identity = 47/139 (33.81%), Postives = 75/139 (53.96%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFVV 666
            E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+    F+ + +   +RVS  VSK+ ++    +PS + 
Sbjct:  293 EYLQLSLEEAFFLAYALGCLSI--YYEKEPLTIVKLWQ-AFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDNFEGSLRRPFSWKSLAALSRVSGNVSKELMLCYLIKPSTMT 428
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Match: sp|Q8NCE0|SEN2_HUMAN (tRNA-splicing endonuclease subunit Sen2 OS=Homo sapiens OX=9606 GN=TSEN2 PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.049e-13
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSIINISCRPS 657
            E L LS  E  F+ Y    ++I  YY K  +++  +W+   T     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+ ++       G+     + + +   +RVS  VSK+ ++    +PS
Sbjct:  295 EYLQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWK-AFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPS 427
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Match: sp|Q5ZIN2|SEN2_CHICK (tRNA-splicing endonuclease subunit Sen2 OS=Gallus gallus OX=9031 GN=TSEN2 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 6.496e-12
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI 531
            E L LS  E  F+ Y    + +  YY +  +++  +W +  +     F + Y+AYHY+RS GWV + GL++GTD LLY   P   HAS+S+
Sbjct:  291 EYLQLSLEEAFFLVYALGCLTV--YYGEEPLTILKLW-EIFSEVKPSFKTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSV 378
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Match: sp|Q74ZY5|SEN2_ASHGO (tRNA-splicing endonuclease subunit SEN2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEN2 PE=3 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 2.151e-11
Identity = 34/93 (36.56%), Postives = 46/93 (49.46%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINV 537
            E L L  +E +F+      ++ +       I    +          R   LY AYH+YRSHGW VRSG++FG D+LLY   P   HA FS+ V
Sbjct:  195 EKLELMPVEALFLTLALPVLHADA----PAILARTLGPQPALPQIERLCRLYAAYHHYRSHGWCVRSGIKFGCDFLLYRRGPPFHHAEFSVMV 283
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Match: A0A1Y1VSE0 (tRNA-splicing endonuclease subunit Sen2 OS=Anaeromyces robustus OX=1754192 GN=BCR32DRAFT_273191 PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 5.653e-17
Identity = 62/162 (38.27%), Postives = 85/162 (52.47%), Query Frame = 1 
Query:  172 EFLIYCGSFGKLMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIW---RDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFL----NGNTLDHF-TVEEMLDQARVSNAVSKKSI 633
            E LI      K + SSD+L+         E L L  +E  F+KY  N I+I       E+S++N W   R  + N    FI  YVAYHYYRS GW+V+ GL++G DY+LY   P  SH+ F+I++   L    N N ++   TV E+    RV N V KK +
Sbjct:  152 ENLIIKKVKQKCIKSSDLLN--------MEYLQLDLVEAFFLKYALNCISIK--NKNKEMSIDNCWNKFRKDINNPADLFILKYVAYHYYRSQGWIVKEGLKYGVDYVLYQKGPAFSHSEFAISIIPVLEDQDNLNYINKLPTVHEINCTNRVCNQVLKKLV 303
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Match: A0A3Q0IQD0 (tRNA-splicing endonuclease subunit Sen2 OS=Diaphorina citri OX=121845 GN=LOC113466917 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.077e-16
Identity = 57/199 (28.64%), Postives = 96/199 (48.24%), Query Frame = 1 
Query:   82 ERHPSIDFCKGKIAVLKKQS--IYFVDLPGHT------EFLIYCGSFGKLMNSSDILSNLDPNENFPELLTLSDI------ETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSII 636
            +R  S+D C       K++S   Y   +P +T      E ++   S  + M++   ++ L+P  +   +LT+ +       E  F+ +  + I I    TK  + + ++W+    NN   F+  YV YHY+RS GW+V+SGL FG D+ LY   P + HAS  + ++  LN       + E M+   RVS A  K  ++
Sbjct:  154 DRSTSLDCCDKIFPKSKQESNLTYEQTMPKYTANETQSEVIVVNDSDDECMDARTFVNKLNPTWHAEPILTVQETLCLTFEEAFFLSFALHCITIKHMSTKQVLYLNDLWK-IFINNDPNFVEKYVTYHYFRSKGWIVKSGLNFGCDFALYKRGPAYYHASCLVKIR-NLNHQDKPKLSWEHMMGYCRVSEAAKKDFVV 350
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Match: A0A1Y1VMU5 (Uncharacterized protein OS=Piromyces finnis OX=1754191 GN=BCR36DRAFT_578840 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.070e-15
Identity = 47/134 (35.07%), Postives = 72/134 (53.73%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIW---RDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLD------QARVSNAVSKKSI 633
            E L L   E  F+KY  N I+I        +S+++ W   R+ ++++  +FI  YV YHYYRS GW+V+ GL++G DY+LY   P  SH+ +++++   +N     H  VE M          RV N V KK +
Sbjct:   26 EYLQLDLQEAFFLKYALNCISIRD--NHKVLSIDDCWKIFRNNISDSSDQFILKYVVYHYYRSQGWIVKEGLKYGVDYVLYQKGPAFSHSEYAVSIIPVINNQDNQHNYVENMPSIHEINCTNRVCNQVLKKLV 157
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Match: A0A2R7VXN4 (tRNA-splicing endonuclease subunit Sen2 OS=Oncopeltus fasciatus OX=7536 GN=OFAS_OFAS002678 PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.440e-15
Identity = 59/172 (34.30%), Postives = 91/172 (52.91%), Query Frame = 1 
Query:  178 LIYCGSFGKLMNSSDILSNLDPN------ENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTL--DHFTVEEMLDQARVSNAVSKKSIINISCRPSFVVK 669
            L+    +G  +N  + L NL+P       E   E+L LS  E  F+ Y  N + +   + ++ IS + +W+     N   FI  YVAYH++RS GWVV+SGL+FG D+LLY   P + HAS+ + VK      T   +  T  EML   R+S + SK+ +I    +P+  ++
Sbjct:  267 LLVINDYG--LNLEESLINLNPRIEKKKIETVLEVLYLSFEEAFFLSYALNCLQVVDLF-QNVISNKELWK-LFHENQPDFIEKYVAYHHFRSKGWVVKSGLKFGGDFLLYKKGPAYYHASYIVLVKILNADGTQASNSVTWGEMLTLNRMSESASKEVLICEVMKPALPIE 434
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Match: K1R4D1 (tRNA-splicing endonuclease subunit Sen2 OS=Crassostrea gigas OX=29159 GN=CGI_10012433 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.432e-14
Identity = 44/120 (36.67%), Postives = 70/120 (58.33%), Query Frame = 1 
Query:  322 INCYYTKSEISVENIWRD-CVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNG-----NTLDHFTVEEMLDQARVSNAVSKKSIINISCRPSFV 663
            +  Y  +  +++ ++WR  C  +N  RFISLYVAYHY+RS GWV +SGL+FGTD+++Y   P   H S+S+ VK   +      + L   T  ++    R++  V+K+ +I    RP+ V
Sbjct:   77 LRVYENEKLLNLTDLWRAFCQRHN--RFISLYVAYHYFRSKGWVPKSGLKFGTDFIIYKEGPPFYHGSYSVIVKMVRDDDLKEEDNLPTLTWTQIAGLNRLTEHVAKQLLICYVIRPTHV 194
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Cavefish
Match: tsen2 (tRNA splicing endonuclease subunit 2 [Source:NCBI gene;Acc:103029398])

HSP 1 Score: 76.2554 bits (186), Expect = 2.384e-15
Identity = 53/165 (32.12%), Postives = 76/165 (46.06%), Query Frame = 1 
Query:  205 LMNSSDILSNLDPNE------------------NFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI---NVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSII 636
            L+   D   N DPNE                  N  E L LS  E  F+ Y    ++I  YY    +SV  +WR    +    F + Y AYHY+RS GWV + G+++GTD +LY   P   HAS+S+    V     G TL  F+       +R++  VSK+ ++
Sbjct:  271 LVEEPDEEDNGDPNEDDISCVRMAKLVCRINPFNMVEHLQLSYEEAFFLVYALGCLSI--YYDGEALSVLQLWR-MFCSVQPNFETNYAAYHYFRSKGWVPKPGIKYGTDLMLYRKGPPFYHASYSVVVEKVDDSFQGATLRPFSWRSFAAISRITGNVSKELML 432
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Cavefish
Match: tsen2 (tRNA splicing endonuclease subunit 2 [Source:NCBI gene;Acc:103029398])

HSP 1 Score: 75.8702 bits (185), Expect = 2.978e-15
Identity = 53/165 (32.12%), Postives = 76/165 (46.06%), Query Frame = 1 
Query:  205 LMNSSDILSNLDPNE------------------NFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI---NVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSII 636
            L+   D   N DPNE                  N  E L LS  E  F+ Y    ++I  YY    +SV  +WR    +    F + Y AYHY+RS GWV + G+++GTD +LY   P   HAS+S+    V     G TL  F+       +R++  VSK+ ++
Sbjct:  271 LVEEPDEEDNGDPNEDDISCVRMAKLVCRINPFNMVEHLQLSYEEAFFLVYALGCLSI--YYDGEALSVLQLWR-MFCSVQPNFETNYAAYHYFRSKGWVPKPGIKYGTDLMLYRKGPPFYHASYSVVVEKVDDSFQGATLRPFSWRSFAAISRITGNVSKELML 432
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Cavefish
Match: tsen2 (tRNA splicing endonuclease subunit 2 [Source:NCBI gene;Acc:103029398])

HSP 1 Score: 73.9442 bits (180), Expect = 1.286e-14
Identity = 49/147 (33.33%), Postives = 72/147 (48.98%), Query Frame = 1 
Query:  205 LMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI---NVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSII 636
            L+   D   N DPNE+        DI   F   VY    ++ YY    +SV  +WR    +    F + Y AYHY+RS GWV + G+++GTD +LY   P   HAS+S+    V     G TL  F+       +R++  VSK+ ++
Sbjct:  271 LVEEPDEEDNGDPNED--------DISCAFF-LVYALGCLSIYYDGEALSVLQLWR-MFCSVQPNFETNYAAYHYFRSKGWVPKPGIKYGTDLMLYRKGPPFYHASYSVVVEKVDDSFQGATLRPFSWRSFAAISRITGNVSKELML 407
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Yeast
Match: SEN2 (Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease; [Source:SGD;Acc:S000004095])

HSP 1 Score: 63.929 bits (154), Expect = 3.785e-12
Identity = 35/93 (37.63%), Postives = 51/93 (54.84%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININ--CYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSI 531
            E L L  +E +F+ +    ++I+  C   K     +  ++D  +     F+  YV YH+YRSHGW VRSG++FG DYLLY   P   HA F +
Sbjct:  216 ESLELMPVEAMFLTFALPVLDISPACLAGKL-FQFDAKYKDIHS-----FVRSYVIYHHYRSHGWCVRSGIKFGCDYLLYKRGPPFQHAEFCV 302
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Nematostella
Match: EDO40117 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S826])

HSP 1 Score: 42.743 bits (99), Expect = 6.268e-6
Identity = 18/48 (37.50%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:  340 KSEISVENIWRD-CVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTD 480
            + E+++ ++W+  C  +    F+  Y+ YHY RS GWV ++GL++GTD
Sbjct:   25 EREMNLNDMWQAFCAAS--PDFLQKYIVYHYLRSKGWVPKTGLKYGTD 70
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Medaka
Match: ENSORLT00000044283.1 (tRNA splicing endonuclease subunit 2 [Source:NCBI gene;Acc:101158936])

HSP 1 Score: 65.4698 bits (158), Expect = 8.488e-12
Identity = 43/131 (32.82%), Postives = 69/131 (52.67%), Query Frame = 1 
Query:  259 ELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVR--SGLRFGTDYLLYVASPEHSHASFSINVKCF---LNGNTLDHFTVEEMLDQARVSNAVSKKSII 636
            E L LS  E  F+ Y    +++  Y  +   SV  +WR   +     F S Y AYH++RS GWV +  SG ++G D++LY   P   HAS+S+ V+       G+TL  F+       +R++ +VSK+ ++
Sbjct:  229 EYLQLSLEEAFFLVYSLGCLSV--YLDQEPQSVIQLWRRFRSLR-PDFTSSYAAYHHFRSRGWVPKGGSGAKYGVDFMLYRKGPPFYHASYSVVVEQTDDEFRGSTLRPFSWRSFAALSRITASVSKELML 356
[Close]
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Planmine SMEST
Match: SMESG000065232.1 (SMESG000065232.1)

HSP 1 Score: 412.535 bits (1059), Expect = 2.559e-147
Identity = 199/203 (98.03%), Postives = 200/203 (98.52%), Query Frame = 1 
Query:   25 MNFKRVCAAKSNYREIILGERHPSIDFCKGKIAVLKKQSIYFVDLPGHTEFLIYCGSFGKLMNSSDILSNLDPNENFPELLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQARVSNAVSKKSI 633
            MNFKRVCAAKSNYREIILGERHPSIDFCKGKIAVLKKQSIYFVDLPGHTEFLIYCGSFGKLMNSSDILSNLD NENFPE+LTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQARVSNAVSK  I
Sbjct:    1 MNFKRVCAAKSNYREIILGERHPSIDFCKGKIAVLKKQSIYFVDLPGHTEFLIYCGSFGKLMNSSDILSNLDLNENFPEVLTLSDIETIFIKYVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWVVRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQARVSNAVSKTFI 203

HSP 2 Score: 88.5817 bits (218), Expect = 1.100e-20
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 3 
Query:  648 QTFICCKVQINTQKDLTDISVLDDCAVEELQISRFDPNAEIH 773
            +TFICCKVQINTQKDLTDISVLDDCAVEELQISRFDPNAEIH
Sbjct:  200 KTFICCKVQINTQKDLTDISVLDDCAVEELQISRFDPNAEIH 241
The following BLAST results are available for this feature:
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TSEN21.478e-1433.82tRNA splicing endonuclease subunit 2 [Source:HGNC ... [more]
TSEN22.185e-1433.82tRNA splicing endonuclease subunit 2 [Source:HGNC ... [more]
TSEN22.185e-1433.82tRNA splicing endonuclease subunit 2 [Source:HGNC ... [more]
TSEN23.130e-1332.06tRNA splicing endonuclease subunit 2 [Source:HGNC ... [more]
TSEN22.141e-1234.19tRNA splicing endonuclease subunit 2 [Source:HGNC ... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tsen-22.025e-630.77Trna (tRNA) Splicing ENdonuclease subunit related ... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG318124.565e-1128.85gene:FBgn0051812 transcript:FBtr0290062[more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tsen25.398e-1631.85TSEN2 tRNA splicing endonuclease subunit [Source:Z... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tsen26.200e-1732.28tRNA splicing endonuclease subunit 2 [Source:MGI S... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6P7W5|SEN2_MOUSE4.337e-1632.28tRNA-splicing endonuclease subunit Sen2 OS=Mus mus... [more]
sp|Q5M954|SEN2_RAT2.532e-1533.81tRNA-splicing endonuclease subunit Sen2 OS=Rattus ... [more]
sp|Q8NCE0|SEN2_HUMAN1.049e-1333.82tRNA-splicing endonuclease subunit Sen2 OS=Homo sa... [more]
sp|Q5ZIN2|SEN2_CHICK6.496e-1237.36tRNA-splicing endonuclease subunit Sen2 OS=Gallus ... [more]
sp|Q74ZY5|SEN2_ASHGO2.151e-1136.56tRNA-splicing endonuclease subunit SEN2 OS=Ashbya ... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1Y1VSE05.653e-1738.27tRNA-splicing endonuclease subunit Sen2 OS=Anaerom... [more]
A0A3Q0IQD05.077e-1628.64tRNA-splicing endonuclease subunit Sen2 OS=Diaphor... [more]
A0A1Y1VMU54.070e-1535.07Uncharacterized protein OS=Piromyces finnis OX=175... [more]
A0A2R7VXN45.440e-1534.30tRNA-splicing endonuclease subunit Sen2 OS=Oncopel... [more]
K1R4D13.432e-1436.67tRNA-splicing endonuclease subunit Sen2 OS=Crassos... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tsen22.384e-1532.12tRNA splicing endonuclease subunit 2 [Source:NCBI ... [more]
tsen22.978e-1532.12tRNA splicing endonuclease subunit 2 [Source:NCBI ... [more]
tsen21.286e-1433.33tRNA splicing endonuclease subunit 2 [Source:NCBI ... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
SEN23.785e-1237.63Subunit of the tRNA splicing endonuclease; tRNA sp... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO401176.268e-637.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000044283.18.488e-1232.82tRNA splicing endonuclease subunit 2 [Source:NCBI ... [more]
back to top
BLAST of tRNA-splicing endonuclease subunit Sen2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000065232.12.559e-14798.03SMESG000065232.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006513 ID=SMED30006513|Name=tRNA-splicing endonuclease subunit Sen2|organism=Schmidtea mediterranea sexual|type=transcript|length=824bp
TCATATTTCATTCAATTAATTGAAATGAATTTTAAACGAGTTTGCGCCGC
AAAAAGTAACTACCGTGAAATAATTCTTGGTGAAAGACATCCATCTATTG
ACTTTTGCAAAGGAAAAATAGCTGTATTAAAAAAACAGTCAATTTATTTT
GTAGATTTACCAGGCCATACTGAATTTTTAATATACTGTGGATCTTTCGG
AAAACTTATGAATTCTTCTGACATACTTTCAAATTTGGATCCAAATGAAA
ATTTCCCAGAACTTCTAACTTTATCAGACATAGAAACTATTTTCATAAAA
TATGTTTATAATAGTATTAATATTAATTGCTATTATACAAAATCAGAGAT
CTCAGTGGAAAATATCTGGCGGGATTGTGTAACTAATAATGGGGCCAGAT
TCATTTCATTATATGTTGCATATCATTATTATCGGTCGCATGGGTGGGTC
GTGCGTTCGGGATTGAGATTCGGAACAGATTATTTATTGTACGTAGCTAG
TCCTGAGCATTCTCATGCATCTTTCAGCATTAACGTGAAATGTTTTCTGA
ATGGTAATACACTTGATCATTTCACTGTCGAAGAGATGCTCGACCAAGCG
CGAGTCAGCAATGCCGTGTCAAAGAAATCAATAATAAACATCTCATGCAG
ACCTTCATTTGTTGTAAAGTGCAAATCAATACCCAAAAGGATCTCACAGA
TATTTCCGTATTGGATGATTGCGCAGTGGAAGAGTTACAAATTTCCAGAT
TCGATCCAAATGCTGAGATTCATTGAAAATCTAAATGATTTTTTTTAATA
AATAAATTATTGATTTTTTAAAAA
back to top

protein sequence of SMED30006513-orf-1

>SMED30006513-orf-1 ID=SMED30006513-orf-1|Name=SMED30006513-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=266bp
SYFIQLIEMNFKRVCAAKSNYREIILGERHPSIDFCKGKIAVLKKQSIYF
VDLPGHTEFLIYCGSFGKLMNSSDILSNLDPNENFPELLTLSDIETIFIK
YVYNSININCYYTKSEISVENIWRDCVTNNGARFISLYVAYHYYRSHGWV
VRSGLRFGTDYLLYVASPEHSHASFSINVKCFLNGNTLDHFTVEEMLDQA
RVSNAVSKKSIINISCRPSFVVKCKSIPKRISQIFPYWMIAQWKSYKFPD
SIQMLRFIENLNDFF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004519endonuclease activity
GO:0004518nuclease activity
GO:0003676nucleic acid binding
GO:0000213tRNA-intron endonuclease activity
GO:0016829lyase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR006676tRNA_splic
IPR036167tRNA_intron_Endo_cat-like_sf
IPR011856tRNA_endonuc-like_dom_sf
IPR006677tRNA_intron_Endonuc_cat-like
Vocabulary: biological process
TermDefinition
GO:0006388tRNA splicing, via endonucleolytic cleavage and ligation
GO:0000379tRNA-type intron splice site recognition and cleavage
GO:0006397mRNA processing
GO:0008033tRNA processing
GO:0090305nucleic acid phosphodiester bond hydrolysis
GO:0090501RNA phosphodiester bond hydrolysis
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
Vocabulary: cellular component
TermDefinition
GO:0000214tRNA-intron endonuclease complex
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005730nucleolus
GO:0005813centrosome
GO:0005829cytosol
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006676tRNA-splicing endonucleaseTIGRFAMTIGR00324TIGR00324coord: 87..214
e-value: 4.4E-18
score: 63.7
IPR006676tRNA-splicing endonucleasePANTHERPTHR21227TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2coord: 87..219
IPR011856tRNA endonuclease-like domain superfamilyGENE3DG3DSA:3.40.1350.10coord: 133..227
e-value: 6.9E-19
score: 69.7
IPR006677tRNA intron endonuclease, catalytic domain-likePFAMPF01974tRNA_int_endocoord: 134..214
e-value: 2.1E-15
score: 56.5
IPR036167tRNA intron endonuclease, catalytic domain-like superfamilySUPERFAMILYSSF53032tRNA-intron endonuclease catalytic domain-likecoord: 133..214