SMED30006482

Overview
NameSMED30006482
Smed IDSMED30006482
Length (bp)1347
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006482 (SMED30006482) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006482 (SMED30006482) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006482

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30006482SMESG000055742.1 dd_Smed_v4_63060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006482SMESG000055742.1 dd_Smed_v4_63060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30006482SMESG000055742.1 dd_Smed_v4_63060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30006482 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.014497:10855..12201 -6717
Homology
[Close]
BLAST of SMED30006482 vs. TrEMBL
Match: A0A193KUC7 (GCR117 OS=Schmidtea mediterranea OX=79327 GN=gcr117 PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 2.546e-51
Identity = 145/357 (40.62%), Postives = 207/357 (57.98%), Query Frame = -1 
Query:  106 KSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVGRKPKNRSI 1176
            K NYSYID+PYD+LY C  + EN   +    C +S I G  S YIFP     G +SN+ ICYIF+ +Y K+TRQTILL+ L+ ADLL IL+ GWLW+FPAKGLPY + G IY FIIN S   C +FR    F+ +  F  FLLVCLDR  AIY+P + +    K       ++   C+  + +   F+R+I      FCT  +  D     FL  +R+L ++SG +  + I++ NL L+ K++   +   +L +      +REI++T+ +LILSL+FL GSLP     +I+  LQ    +ST  Q+ LQ LY L D     + ++ES +F +Y  +MK+F++  L       KN SI
Sbjct:    8 KLNYSYIDSPYDMLYLCDPKTENININ----CAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNK-DKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQN---DSTINQNYLQFLYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALIL---SNSTKNLSI 353
[Close]
BLAST of SMED30006482 vs. TrEMBL
Match: A0A183SIW2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4160 PE=4 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 6.720e-45
Identity = 119/350 (34.00%), Postives = 200/350 (57.14%), Query Frame = -1 
Query:  133 NYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCI-CCSTVM---NVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMV 1170
            N+ Y+D+P+DLL  C      N  +    CVLSHI GI S YIFPI  +FG +SN L+ Y+F+  + + +RQ I L  ++ AD++ I+S GW+WMFPAKGLPYA+ G +Y F++N++D SC++FR   SF+       FL+   DR L +Y+P+K      K A  A    +GL I CCS +M    +  R  K   K+ C    G  V     L+ + +L  NS  +Q  L+++VN+ L+ ++ ++    + +++C      +EI A+++++ILS + ++ +LP     YI+  +    L     +  +++ + + DLG H +FF +S N+ +Y  +MK+F+   + ++
Sbjct:    8 NWLYVDSPWDLLRDCKDYQTGN-STKFGACVLSHILGITSAYIFPIVGTFGVVSNFLVFYVFLFVFRRSSRQMIYLGCVAIADMMTIVSFGWMWMFPAKGLPYATSGRVYFFVLNVNDLSCKVFRYVYSFTSCLSSSLFLVTACDRCLCVYFPLKFSRLSKKRAWQA----VGLTILCCSLLMLPYPILARHDKFQGKMICWVHEGEKV-----LQIYHVLLANSSLLQTFLVIIVNIALVIRLRQSAALREAMSKCVS--VSKEIAASMLLVILSTMVVICALPQSV-AYILSTILAKVLEGEHGRMVVRMTFNVSDLGWHLLFFQQSFNWLLYMKRMKNFRRANMKLL 344
[Close]
BLAST of SMED30006482 vs. TrEMBL
Match: A0A3P6PGL7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS266 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.243e-37
Identity = 108/361 (29.92%), Postives = 202/361 (55.96%), Query Frame = -1 
Query:   94 SNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVG-RKPKNRSIYTMS 1173
            +++ YID+P+DL   C   + +  + +   C+++H+ GI+S YIFP   +FG ISN L+ Y+F   Y K TRQ I L  ++ AD+L +++ GW+W+FPAKGLPYA+   +Y FI N +D +C++FR   SF+ S     FLL  LDR L+IY+P++      + A  A L++  +         + +       KI C     + +    FL+ + +L  NS  +Q +L++ VN+ L+ ++ +   + + ++ C+     REI A++++++LS   ++ +LP     Y +  +    L+  +    ++++Y + DL  H +F  ++ N+ +Y  +MK+F+ + L ++  R+  +R ++  S
Sbjct:    7 TDWPYIDSPWDLFLECDRYN-STVRKNFGACIIAHMLGIVSAYIFPFIGAFGLISNFLVLYVFFFVYRKPTRQMIYLGSIAGADILTLITFGWIWLFPAKGLPYATLARVYFFIFNFNDVTCKVFRYAYSFTASLSSSLFLLTALDRCLSIYFPLRFSRISTRRAWEAVLIITLVSALAMLPFGLMVGHSLSQGKIIC-----WVLEQKTFLQIYHVLLSNSSLLQTLLVIGVNIALVVRLRRAVSERRSMSNCTTT--NREIAASMLLVMLSGATVMAALPQSIA-YALSSILAEVLHGDEGLTIVRLVYNISDLCWHILFLQQALNWVLYMKRMKNFREQTLRLLRCRRQASRGLHEES 358
[Close]
BLAST of SMED30006482 vs. TrEMBL
Match: U6J3B5 (Rhodopsin orphan GPCR OS=Echinococcus granulosus OX=6210 GN=EGR_02845 PE=4 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 3.863e-37
Identity = 121/359 (33.70%), Postives = 203/359 (56.55%), Query Frame = -1 
Query:  133 KDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQL--CVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIG---SKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMV 1194
            + +L    N+ Y+D+P+DLL  C   + + P + T L  CVLSH+ G +S YIFPI   FG +SNL I YIF+  + K +RQ I LA ++ AD++ I+  GW+WMFPAKGLPYA+   +Y FI N++++SC++ R   SFS       FLL   DR L IY+P+K      + A  A  V   + +  + VM  F   ++ G    KI C      +      L+ + +L  NS  +Q +L++++N+ LL ++ ++    + +++C+     REI A+++++ILS + ++ +LP     YI   +    LN    +  ++I Y + DLG   +FF +++N+ +Y  +MK+F+   L ++
Sbjct:    8 RSILPEDPNWPYLDSPWDLLRDCQDYNSDAPNASTLLSACVLSHMLGFVSAYIFPIVGLFGVVSNLFITYIFLFVFRKPSRQMIYLACVAAADVITIILFGWIWMFPAKGLPYATGARVYFFIFNVNNYSCKVMRYLYSFSSCLSSSLFLLTAFDRCLCIYFPLKFARISRRRAWEAVGV---ITLFSALVMLPFGLLVEHGFSNGKIICWVQVEPNT-----LQIYHVLLANSSLLQTVLVIIINIALLIRLRQSAVLRETMSKCTSA--NREIAASMLLVILSTIVVICALPQSIA-YIFSTILSQMLNGEAGRMAVRIAYNISDLGWQLLFFQQASNWVLYMKRMKNFRRATLRLL 355
[Close]
BLAST of SMED30006482 vs. TrEMBL
Match: A0A068Y9D1 (Rhodopsin orphan GPCR OS=Echinococcus multilocularis OX=6211 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 2.227e-36
Identity = 120/359 (33.43%), Postives = 203/359 (56.55%), Query Frame = -1 
Query:  133 KDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQL--CVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIG---SKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMV 1194
            + +L    N+ Y+D+P+DLL  C   + + P + T L  CVLSH+ G +S YIFPI   FG +SNL I YIF+  + K +RQ I LA ++ AD++ I+  GW+WMFPAKGLPYA+   +Y FI N++++SC++ R   SFS       FLL   DR L IY+P+K      + A  A  V   + +  + VM  F   ++ G    KI C      +      L+ + +L  NS  +Q +L++++N+ LL ++ ++    + +++C+     REI A+++++ILS + ++ +LP     YI   +    L+    +  ++I Y + DLG   +FF +++N+ +Y  +MK+F+   L ++
Sbjct:    8 RSILPEDPNWPYLDSPWDLLRDCQEYNSDAPNASTLLSACVLSHMLGFVSAYIFPIVGFFGVVSNLFITYIFLFVFRKPSRQMIYLACVAAADVITIILFGWIWMFPAKGLPYATGARVYFFIFNVNNYSCKVMRYLYSFSSCLSSSLFLLTAFDRCLCIYFPLKFARISRRRAWEAVGV---ITLFSALVMLPFGLLVEHGFSNGKIICWVQVEPNT-----LQIYHVLLANSSLLQTVLVIIINIALLIRLRQSAVLRETMSKCTSA--NREIAASMLLVILSTIVVICALPQSIA-YIFSTILSQVLHGEAGRMAVRIAYNISDLGWQLLFFQQASNWVLYMKRMKNFRRATLRLL 355
[Close]
BLAST of SMED30006482 vs. Planmine SMEST
Match: SMESG000055742.1 (SMESG000055742.1)

HSP 1 Score: 660.218 bits (1702), Expect = 0.000e+0
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = -1 
Query:   49 MKDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVGRKPKNRSIYTMSNQENIISTNNHTLKR 1197
            MKDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMILIVLVNLLLLWKVIKTHEKTKILAECSMKIFKREIKATVVVLILSLLFLLGSLPGFSCTYIIIYLQGSTLNSTDLQDQLQILYILGDLGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVGRKPKNRSIYTMSNQENIISTNNHTLKR
Sbjct:    1 MKDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMILIVLVNLLLLWKVIKTHEKTKILAECSMKIFKREIKATVVVLILSLLFLLGSLPGFSCTYIIIYLQGSTLNSTDLQDQLQILYILGDLGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVGRKPKNRSIYTMSNQENIISTNNHTLKR 383
[Close]
BLAST of SMED30006482 vs. Planmine SMEST
Match: SMESG000022744.1 (SMESG000022744.1)

HSP 1 Score: 187.193 bits (474), Expect = 7.082e-55
Identity = 145/357 (40.62%), Postives = 207/357 (57.98%), Query Frame = -1 
Query:  106 KSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQSEFLDMVGRKPKNRSI 1176
            K NYSYID+PYD+LY C  + EN   +    C +S I G  S YIFP     G +SN+ ICYIF+ +Y K+TRQTILL+ L+ ADLL IL+ GWLW+FPAKGLPY + G IY FIIN S   C +FR    F+ +  F  FLLVCLDR  AIY+P + +    K       ++   C+  + +   F+R+I      FCT  +  D     FL  +R+L ++SG +  + I++ NL L+ K++   +   +L +      +REI++T+ +LILSL+FL GSLP     +I+  LQ    +ST  Q+ LQ LY L D     + ++ES +F +Y  +MK+F++  L       KN SI
Sbjct:    8 KLNYSYIDSPYDMLYLCDPKTENININ----CAISEILGFSSGYIFPFVGFIGVVSNIYICYIFLFRYPKRTRQTILLSYLALADLLNILAFGWLWIFPAKGLPYMTNGNIYFFIINQSSVYCTVFRTLYFFTSTVTFAIFLLVCLDRCFAIYFPFRSLRITNKMTFLTCSIVFLFCLPLTVLYTGFIRTIYQHPYFFCTIGNK-DKSLNLFLNLYRVLIVHSGVLFTVGIIISNLALILKLVLLSKTNLLLIKTKSNNIQREIRSTITILILSLIFLSGSLPNTCLFFIMKSLQN---DSTINQNYLQFLYSLTDAFYQLLIWIESFSFLVYVIRMKNFRALIL---SNSTKNLSI 353
[Close]
BLAST of SMED30006482 vs. Planmine SMEST
Match: SMESG000022976.1 (SMESG000022976.1)

HSP 1 Score: 139.428 bits (350), Expect = 8.688e-37
Identity = 74/162 (45.68%), Postives = 102/162 (62.96%), Query Frame = -1 
Query:  706 KSNYSYIDTPYDLLYFCHSEDENNPKSDTQ---LCVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKK--TRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMK 1176
            K+ + YID+P+DLL  C  ED N    D +   LC+ SHI G+ S YIFP    FG I N +I Y+F+++Y  K  TRQ I L+ L+ AD   I++ GWLW+FPAKGLPY S+G+IY F  N ++ +C+  R   SF+ +     FLL  +DR L+IY P+K
Sbjct:    6 KNAWLYIDSPWDLLVPC--EDLNTTIEDERTSALCISSHILGVFSGYIFPFVGVFGIIFNFMIFYVFMIRYPPKLRTRQLIYLSALAIADNFSIITFGWLWIFPAKGLPYISKGLIYYFTFNENNPTCKAHRYIYSFASTLAVNLFLLTSIDRCLSIYKPLK 165
[Close]
BLAST of SMED30006482 vs. Planmine SMEST
Match: SMESG000052989.1 (SMESG000052989.1)

HSP 1 Score: 135.961 bits (341), Expect = 1.092e-35
Identity = 124/346 (35.84%), Postives = 187/346 (54.05%), Query Frame = -1 
Query:  151 LKSNYSYIDTPYDLLYFCHSEDENNPKSDTQL-CVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSA-VYASLVMLGLCICCSTVMNVFLRSIKIGSKIFCTATSGYDVHTI-EFLKTWRLLFLNSGAIQMXXXXXXXXXXXXXXIKTHEKTKILAECSMKIFKREIKATXXXXXXXXXXXXXXXXXXSCTYIIIYLQGSTLNSTXXXXXXXXXXXXXXXGVHFIFFMESTNFFIYYFKMKHFQS 1179
            LK+ + +ID+P+DLL  C          +  L CV S I G  S ++FP+ + FG   N+LI YIF  K+  KTRQ I L+ L+  D   ++  GW+W+F AKGLPYAS G++Y F  N     C+L R F +         FLL  +DR  AI++P+K +N   K A  Y+S+V+L   I  S  +     S     K  C   +  D  T+  FL+ + +LF   G+ Q + I+LVNL L ++V K  +  + +   + K  ++EI A V++L+LS+LF +GSLP      +   +Q  T    D   +L+ +Y +GD+G H     + TN  +YY+KM HF+S
Sbjct:    9 LKAQWKFIDSPWDLLISCEEVKLKQTNENNYLFCVFSQIMGFWSAFVFPVISIFGIFLNILIIYIFNFKFRDKTRQYIYLSFLAAFDSFNLICSGWMWIFFAKGLPYASSGVLYFFTYNQGVIPCKLHRYFTAAISCSAISIFLLTSIDRYFAIFWPLKCLNIPLKKAWQYSSMVVLLSFIMMSPFIVYMDHSETGNHKTICWLEASKDKETLGTFLQIYNVLFSFCGSFQTLAIILVNLSLFYQVCKGQKLREQIQAANCKSIRKEISACVILLVLSVLFTVGSLPTSISFLLAFLMQNDT--GPDADQRLRFVYNIGDIGWHLYMVQQCTNGLVYYWKMPHFRS 352
[Close]
BLAST of SMED30006482 vs. Planmine SMEST
Match: SMESG000053055.1 (SMESG000053055.1)

HSP 1 Score: 122.479 bits (306), Expect = 9.358e-31
Identity = 78/235 (33.19%), Postives = 122/235 (51.91%), Query Frame = -1 
Query:  496 KSNYSYIDTPYDLLYFCHSEDENNPKSDTQL-CVLSHIFGIISVYIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLGWLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGF-----CSFSFSWIFGAFLLVCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKIGS-KIFCTATSGYDVHTIE-FLKTWRLLFLNSGAIQ 1176
            K+ + Y+D+P+DLL  C     +    +T + C+ SHI G  S YI PI + FG +SN++  YI   K   +TRQ I L+ ++  D + I+  GW+W+F AKGLPYAS G +  F  N  + SC+L R F     CS S       FLL  +DR  AI++P+K +N   + A   S+ ++       +   +++   + G+ K  C      D   +  FL+ + +LF   G+ Q
Sbjct:   10 KARWKYVDSPWDLLTACEELRLSKTTGNTLVFCIFSHIMGFWSAYILPIISLFGIVSNMVTIYILSSKLHDRTRQYIYLSFIAGCDSVNIICYGWIWIFFAKGLPYASLGKVSFFTYNQGEISCKLHRYFIAAISCSAS-----SIFLLASVDRWFAIFWPLKCLNIPLRRAWQYSIGVIISSFILMSPFAIYMGYTETGNQKTLCWIKVSTDEQGLAIFLQVYNVLFSFCGSFQ 239
The following BLAST results are available for this feature:
BLAST of SMED30006482 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUC72.546e-5140.62GCR117 OS=Schmidtea mediterranea OX=79327 GN=gcr11... [more]
A0A183SIW26.720e-4534.00G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P6PGL71.243e-3729.92G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
U6J3B53.863e-3733.70Rhodopsin orphan GPCR OS=Echinococcus granulosus O... [more]
A0A068Y9D12.227e-3633.43Rhodopsin orphan GPCR OS=Echinococcus multilocular... [more]
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BLAST of SMED30006482 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006482 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055742.10.000e+0100.00SMESG000055742.1[more]
SMESG000022744.17.082e-5540.62SMESG000022744.1[more]
SMESG000022976.18.688e-3745.68SMESG000022976.1[more]
SMESG000052989.11.092e-3535.84SMESG000052989.1[more]
SMESG000053055.19.358e-3133.19SMESG000053055.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006482 ID=SMED30006482|Name=SMED30006482|organism=Schmidtea mediterranea sexual|type=transcript|length=1347bp
TCCAGAAATATTTATTTAAACAATTAATGAATGTTTTAATATTAATTATC
GTTTTAAAGTATGATTATTTGTCGAAATAATATTTTCTTGATTAGACATT
GTATAAATTGACCTATTTTTCGGTTTCCTGCCCACCATATCCAAGAATTC
ACTTTGGAAATGTTTCATTTTAAAATAATATATAAAGAAATTTGTCGATT
CCATAAAAAATATAAAATGAACTCCTAGGTCACCGAGAATATAAAGAATC
TGAAGTTGATCCTGCAAATCGGTACTGTTCAATGTACTACCTTGCAAGTA
TATGATAATATATGTGCACGAAAACCCTGGCAAGCTTCCTAATAGAAATA
GTAGAGACAAAATTAACACAACAACTGTCGCTTTAATTTCTCTTTTAAAA
ATCTTCATGGAACATTCTGCCAAAATCTTAGTCTTTTCGTGGGTTTTAAT
TACTTTCCATAGCAACAGCAAATTAACTAACACTATTAGGATCATTTGAA
TGGCACCAGAATTTAGAAAGAGTAATCGCCAGGTCTTCAGAAACTCAATA
GTATGGACATCGTAACCTGAAGTGGCAGTGCAGAAAATTTTGGAGCCAAT
CTTGATCGATCTCAAAAACACATTCATGACAGTGCTGCAGCAAATACACA
GCCCAAGCATCACCAATGAAGCGTAGACTGCTGATTTGTATCCGAAATTT
ATCATTTTCATTGGATAATATATAGCTAACATTCGGTCCAGACAAACCAG
CAAGAATGCTCCAAATATCCAAGAAAAAGAAAACGAGCAGAAACCTCGAA
AAAGTCGACATGAGAAATCGGATAAGTTTATAATAAATAAATAAATCATT
CCTTGACTTGCGTACGGTAGCCCTTTTGCGGGAAACATCCACAGCCATCC
TAAAGAAAGAATAAACAATAAATCGGCAAAAGACAAAACTGCCAGTAAAA
TCGTCTGTCGGGTTTTCTTGGAATATTTCACTATGAAAATGTAGCAAATC
AGCAAATTGCTTATGAAACCGAAACTGGCAAAAATTGGGAAAATGTATAC
TGAAATTATCCCAAAAATGTGGCTCAAAACGCACAGTTGCGTATCGGATT
TGGGATTATTCTCGTCCTCAGAATGGCAAAAATAGAGCAAATCGTACGGA
GTATCAATGTAGCTGTAATTGGATTTTAATGTTAAAAGATCTTTCATTTT
TAAAAAATTAAAATAACAGTGTGAAAATTAATAATAAAAATCATAAATAT
TTATTTTTTTAAATAATCCGAATACTGAGTACTTTCCTATTGGCTGTCAA
ATAGGAGAATTACGATTGGATAAGAATTGTTCTACAAATGGGCAAAT
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protein sequence of SMED30006482-orf-1

>SMED30006482-orf-1 ID=SMED30006482-orf-1|Name=SMED30006482-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=384bp
MKDLLTLKSNYSYIDTPYDLLYFCHSEDENNPKSDTQLCVLSHIFGIISV
YIFPIFASFGFISNLLICYIFIVKYSKKTRQTILLAVLSFADLLFILSLG
WLWMFPAKGLPYASQGMIYLFIINLSDFSCRLFRGFCSFSFSWIFGAFLL
VCLDRMLAIYYPMKMINFGYKSAVYASLVMLGLCICCSTVMNVFLRSIKI
GSKIFCTATSGYDVHTIEFLKTWRLLFLNSGAIQMILIVLVNLLLLWKVI
KTHEKTKILAECSMKIFKREIKATVVVLILSLLFLLGSLPGFSCTYIIIY
LQGSTLNSTDLQDQLQILYILGDLGVHFIFFMESTNFFIYYFKMKHFQSE
FLDMVGRKPKNRSIYTMSNQENIISTNNHTLKR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likeSMARTSM013817TM_GPCR_Srsx_2coord: 57..355
e-value: 0.0046
score: -64.5
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 64..319
e-value: 4.8E-10
score: 39.2
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 41..355
e-value: 2.7E-25
score: 91.0
NoneNo IPR availablePANTHERPTHR24246OLFACTORY RECEPTOR AND ADENOSINE RECEPTORcoord: 42..359
NoneNo IPR availablePANTHERPTHR24246:SF27ADENOSINE RECEPTOR, ISOFORM Acoord: 42..359
NoneNo IPR availableCDDcd149827tmA_purinoceptor-likecoord: 49..197
e-value: 2.87498E-8
score: 52.2673
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 36..365
NoneNo IPR availableTMHMMTMhelixcoord: 319..341
NoneNo IPR availableTMHMMTMhelixcoord: 40..62
NoneNo IPR availableTMHMMTMhelixcoord: 131..153
NoneNo IPR availableTMHMMTMhelixcoord: 83..105
NoneNo IPR availableTMHMMTMhelixcoord: 277..299
NoneNo IPR availableTMHMMTMhelixcoord: 225..247
NoneNo IPR availableTMHMMTMhelixcoord: 173..195
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 63..340
score: 16.721