Acyl-CoA-binding domain-containing protein 5
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006466 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Xenopus
Match: acbd5 (acyl-CoA binding domain containing 5 [Source:Xenbase;Acc:XB-GENE-982910]) HSP 1 Score: 80.8777 bits (198), Expect = 9.280e-18 Identity = 60/161 (37.27%), Postives = 89/161 (55.28%), Query Frame = 2 Query: 314 RINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLM--SINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 R+N L P +RRTV LET H N Q +++ R+H + R +YG +E YR +R+ LK+QMSEK +L ++ + R+ E + V D+ L +D+ R F+ ++RD NK +ME + E L E++LLQ+NPINWS TLK Sbjct: 57 RLNPLHPPGIKRRTVSLETVAAHHHNQQRSLTMQQREYNRYHQGWRRPFYGTPTEKEDYRNDIRQLLKKQMSEKWALQREVLSSQSRELEVLMEV--DRTALMQDVEQQRTHAVFLTKYRDENKRLMETKWHENRLTRSLETLKERELLQFNPINWSGTLK 215
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Match: A0A267F5V4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007885g1 PE=4 SV=1) HSP 1 Score: 113.62 bits (283), Expect = 9.885e-27 Identity = 69/160 (43.12%), Postives = 99/160 (61.88%), Query Frame = 2 Query: 311 KRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 KR+N LRP PPER T ET D H N Q ER+ RFHNA+SRYYYG+ +E++ +Y RE LKQQM + + ++ + ES A RD+ D+E + + + +F+ Q+RD NK++MEE +R + E RE +++ L+YNPINWS +LK Sbjct: 59 KRVNPLRPQPPERMTCVTETGDPHYQNQQRLAMLERKQFHRFHNAWSRYYYGSVAEKELHNRYFREGLKQQMRDSDEKNRRVFREKAQESSVAFSRDRQDIESEQVQRASKHQFLTQYRDANKMMMEEKAQRLRAERQRELQFDREQLKYNPINWSCSLK 218
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Match: A0A1I8I5N0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 113.62 bits (283), Expect = 1.650e-26 Identity = 69/160 (43.12%), Postives = 99/160 (61.88%), Query Frame = 2 Query: 311 KRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 KR+N LRP PPER T ET D H N Q ER+ RFHNA+SRYYYG+ +E++ +Y RE LKQQM + + ++ + ES A RD+ D+E + + + +F+ Q+RD NK++MEE +R + E RE +++ L+YNPINWS +LK Sbjct: 83 KRVNPLRPQPPERMTCVTETGDPHYQNQQRLAMLERKQFHRFHNAWSRYYYGSVAEKELHNRYFREGLKQQMRDSDEKNRRVFREKAQESSVAFSRDRQDIESEQVQRASKHQFLTQYRDANKMMMEEKAQRLRAERQRELQFDREQLKYNPINWSCSLK 242
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Match: A0A1I8I9T9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 113.235 bits (282), Expect = 2.757e-26 Identity = 69/160 (43.12%), Postives = 99/160 (61.88%), Query Frame = 2 Query: 311 KRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 KR+N LRP PPER T ET D H N Q ER+ RFHNA+SRYYYG+ +E++ +Y RE LKQQM + + ++ + ES A RD+ D+E + + + +F+ Q+RD NK++MEE +R + E RE +++ L+YNPINWS +LK Sbjct: 101 KRVNPLRPQPPERMTCVTETGDPHYQNQQRLAMLERKQFHRFHNAWSRYYYGSVAEKELHNRYFREGLKQQMRDSDEKNRRVFREKAQESSVAFSRDRQDIESEQVQRASKHQFLTQYRDANKMMMEEKAQRLRAERQRELQFDREQLKYNPINWSCSLK 260
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Match: R7VIY8 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_228779 PE=4 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 1.607e-23 Identity = 70/198 (35.35%), Postives = 110/198 (55.56%), Query Frame = 2 Query: 197 SNTALQPVPLRR-SYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATL 787 S +AL +P ++ S I ++ +D YP + + K++N L+P PP+RRTV LET D H N Q +++ R+H+A+S+YYYG+ +E YR Y R LKQQM + S + R ES A+ D+ D +DM Q+ +++ FRD NK +ME S + ++ +++ + YNPINWS +L Sbjct: 11 SVSALPRIPHQQASMSISNFTSTVYDTYPGRLPATAVIPKKVNPLQPTPPKRRTVALETIDPHWQNEQRTMRMKQREHHRYHDAWSKYYYGSQPEQEQYRAYTRSVLKQQMRDLWSRKRQDRTDRSKESQAALLHDRQDRMQDMNNAMQKVVYLKTFRDENKKLMENSWQNIRDTKTSSDRFDREQMLYNPINWSHSL 208
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Match: A0A2T7NVT6 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_15779 PE=4 SV=1) HSP 1 Score: 100.908 bits (250), Expect = 4.326e-22 Identity = 68/180 (37.78%), Postives = 111/180 (61.67%), Query Frame = 2 Query: 266 FDPYPAP--TAFSENL--CKRINVLRPIPPERR-TVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 + PYP P T+ +++ +RIN L P P+RR ET DQH N + + +++ RFH+A+S+ +YG+ ++E YRK+ RE LKQQMS++++L K + ES AV D+ L +D ++ ++++Q+RD NK ME ++++ E M E ++++L+YNPINWS TLK Sbjct: 33 YGPYPPPELTSIWDHMKIPQRINPLAPPIPKRRINASWETVDQHWHNDKRRIIMQQREHSRFHSAWSKPFYGSPADQEAYRKHFREVLKQQMSDQDALKRQQLKDKVRESEAAVEYDRQCLVQDYEAFSKKNDYLKQYRDENKRFMESQWQQRRIERMLENHYDREVLRYNPINWSGTLK 212
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Nematostella
Match: EDO34885 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN42]) HSP 1 Score: 51.6026 bits (122), Expect = 3.799e-8 Identity = 52/161 (32.30%), Postives = 83/161 (51.55%), Query Frame = 2 Query: 314 RINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERF---HNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATL 787 + N LRP +RTV LET A Q + K R RE + H Y + YGN + +E R+ R L++Q++E+++ R ES A+ D+A +D R ++++FRD NK +ME ++ + E + E++LL +PINW+ TL Sbjct: 20 KANPLRPGEG-KRTVSLETV---ASQQQVEEHKLRGKREAYMKRHELYVKSMYGNVIEKEQVRQKQRALLQEQIAEQDNKHRQYLDDRYKESNFAIHYDEACRRQDAEDRNNRKAYLQKFRDENKRLMETRAEQLRREREAQHQRERELLALSPINWTKTL 176
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Planmine SMEST
Match: SMESG000041447.1 (SMESG000041447.1) HSP 1 Score: 406.757 bits (1044), Expect = 2.224e-145 Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 2 Query: 125 MKVTESSKLPRMACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 MKVTESSKLPRMACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNKIIMEESMKRKKEELMREKLIEKQLLQYNPINWSATLK Sbjct: 1 MKVTESSKLPRMACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNKIIMEESMKRKKEELMREKLIEKQLLQYNPINWSATLK 222
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Planmine SMEST
Match: SMESG000041447.1 (SMESG000041447.1) HSP 1 Score: 385.185 bits (988), Expect = 6.748e-137 Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 2 Query: 158 MACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNXXXXXXXXXXXXXXXXXXXXXXXQLLQYNPINWSATLK 790 MACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNKIIMEESMKRKKEELMREKLIEKQLLQYNPINWSATLK Sbjct: 1 MACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPYPAPTAFSENLCKRINVLRPIPPERRTVFLETEDQHAINYQSKTEKERQMRERFHNAYSRYYYGNSVNREIYRKYLRESLKQQMSEKNSLMSINYKMRQNESVEAVRRDQADLEKDMLTCRQRTEFMRQFRDGNKIIMEESMKRKKEELMREKLIEKQLLQYNPINWSATLK 211 The following BLAST results are available for this feature:
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Acyl-CoA-binding domain-containing protein 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006466 ID=SMED30006466|Name=Acyl-CoA-binding domain-containing protein 5|organism=Schmidtea mediterranea sexual|type=transcript|length=828bpback to top protein sequence of SMED30006466-orf-1 >SMED30006466-orf-1 ID=SMED30006466-orf-1|Name=SMED30006466-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp MKVTESSKLPRMACPSDSIMMTVRSNTALQPVPLRRSYEIKIPSTNAFDPback to top Annotated Terms
The following terms have been associated with this transcript:
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