Tubulin alpha chain

Overview
NameTubulin alpha chain
Smed IDSMED30006461
Length (bp)1739
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin alpha chain (SMED30006461) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin alpha chain (SMED30006461) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tubulin alpha chain (SMED30006461) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tubulin alpha chain (SMED30006461) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30006461

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 52

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30006461h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30006461h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30006461h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30006461h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
prepharyngeal regionSMED30006461h1SMcG0000478 SmedASXL_008060SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30006461h1SMcG0000478 SmedASXL_008060SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
gutSMED30006461h1SMcG0000479 dd_Smed_v4_18010_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14354uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14354newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig41392newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig41392uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14476uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14476newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig45405uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig45405newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30006461h1SMcG0000479 dd_Smed_v4_18010_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30006461h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14354uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14354newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig41392newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig41392uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14476uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig14476newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig45405uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006461h1SMcG0000479 Contig45405newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30006461h1SMcG0000479 dd_Smed_v6_60553_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30006461h1SMcG0000479 dd_Smed_v6_18010_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30006461h1SMcG0000479 dd_Smed_v6_60553_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30006461h1SMcG0000479 dd_Smed_v6_18010_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
gutSMED30006461h1SMcG0000479 SMED30000639smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30006461h1SMcG0000479 SMED30000639smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
gamma neoblastSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30006461h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1A (tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:20766])

HSP 1 Score: 375.17 bits (962), Expect = 5.973e-125
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   40 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 400          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1C (tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:20768])

HSP 1 Score: 375.941 bits (964), Expect = 9.970e-125
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1B (tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:18809])

HSP 1 Score: 375.555 bits (963), Expect = 1.237e-124
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1A (tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:20766])

HSP 1 Score: 374.785 bits (961), Expect = 3.271e-124
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1A (tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:20766])

HSP 1 Score: 374.785 bits (961), Expect = 3.271e-124
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-9 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:Q20221])

HSP 1 Score: 377.096 bits (967), Expect = 1.346e-125
Identity = 187/361 (51.80%), Postives = 258/361 (71.47%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G Y+ LF+P+ L+   EDAANN+ARG Y+IGK+ +  V+   R+  ENC  +  FL+ HSFGGGTGSGFTA+LME LSV+YGK++K+E  ++P+P +STS+VEPYN++L  H  +E+SD +   +NE+++ +C  NLD    T+TNLNRL+AQ+IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     +T  EI++Q F    QM   DP+ G YM+CCLL+RGD+     N AI ++K K S+ +VDWCPTGFK+GINYQP   VPG+ L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   80 IDEIRTGTYKTLFHPEQLLTGKEDAANNYARGHYTIGKEIIDVVMDRVRRLVENCRGLQGFLVFHSFGGGTGSGFTALLMERLSVDYGKKSKLEFCIYPAPQVSTSMVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDLARPTYTNLNRLIAQVISSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLTTYAPIISAEKAYHEQMTVAEITQQCFEPGAQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIASVKTKRSIQFVDWCPTGFKVGINYQPPTVVPGADLSKLQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 440          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: mec-12 (Tubulin alpha-3 chain Detyrosinated tubulin alpha-3 chain [Source:UniProtKB/Swiss-Prot;Acc:P91910])

HSP 1 Score: 370.163 bits (949), Expect = 6.579e-123
Identity = 185/361 (51.25%), Postives = 253/361 (70.08%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G YR+LF+P+ LI   EDAANN+ARG Y+IGK+ +   +   R+ A+NC  +  FL+ HSFGGGTGSGFT++LME LSV+YGK+ K+E  ++P+P +ST+VVEPYN++L  H  +E+SD +   +NE+++ +C  NLD +  ++TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL +F P +   K     L+  EI+   F   NQM   DP+ G YM+ CLLFRGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L    R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 IDEIRTGTYRSLFHPEQLITGKEDAANNYARGHYTIGKEIIDLTLDRIRRLADNCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKAKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATFSPVISAEKAYHEQLSVAEITNMCFEPHNQMVKCDPRHGKYMAVCLLFRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-4 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5EDD4])

HSP 1 Score: 360.918 bits (925), Expect = 2.542e-119
Identity = 178/361 (49.31%), Postives = 253/361 (70.08%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DEI+ G Y+ LF+P+ +I   EDAANN+ARG Y++GK+ +   +   R+ A+NC  +  F + HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD D  ++TNLNR+++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +   K     L+  +I+   F   NQM   DP+ G YM+ CLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L    R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EF+EA EDL  LEKDYE +
Sbjct:   73 VDEIRTGNYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTTLDRIRRLADNCSGLQGFFVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICKRNLDVDRPSYTNLNRIISQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLASYTPLISAEKAYHEALSVNDITNSCFEPANQMVKCDPRNGKYMAVCLLYRGDVVPKDVNTAITAIKTKRSVQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWSRLDYKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDYEEV 433          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-2 (Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P34690])

HSP 1 Score: 360.533 bits (924), Expect = 3.708e-119
Identity = 176/361 (48.75%), Postives = 254/361 (70.36%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DEI+ G Y+ LF+P+ +I   EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+ CLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L    R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EF+EA EDL  LEKDYE +
Sbjct:   73 VDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEKAYHEALSVSDITNSCFEPANQMVKCDPRHGKYMAVCLLYRGDVVPKDVNTAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWSRLDYKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDYEEV 433          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-2 (Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P34690])

HSP 1 Score: 360.533 bits (924), Expect = 3.708e-119
Identity = 176/361 (48.75%), Postives = 254/361 (70.36%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DEI+ G Y+ LF+P+ +I   EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+ CLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L    R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EF+EA EDL  LEKDYE +
Sbjct:   73 VDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEKAYHEALSVSDITNSCFEPANQMVKCDPRHGKYMAVCLLYRGDVVPKDVNTAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVPRAVCMLSNTTAIAEAWSRLDYKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDYEEV 433          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84D (gene:FBgn0003885 transcript:FBtr0081538)

HSP 1 Score: 377.867 bits (969), Expect = 7.857e-126
Identity = 186/361 (51.52%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM  VDP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKVDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84B (gene:FBgn0003884 transcript:FBtr0081639)

HSP 1 Score: 375.555 bits (963), Expect = 7.766e-125
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub85E (gene:FBgn0003886 transcript:FBtr0082087)

HSP 1 Score: 374.015 bits (959), Expect = 2.416e-124
Identity = 183/361 (50.69%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+ NLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P + + K     LT  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EF+EA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYMNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISVEKAYHEQLTVAEITNACFEPANQMVKCDPRRGKYMACCMLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFAEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub67C (gene:FBgn0087040 transcript:FBtr0076393)

HSP 1 Score: 334.339 bits (856), Expect = 1.055e-108
Identity = 167/361 (46.26%), Postives = 245/361 (67.87%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            ID+++ G  R L++P+ LI+  EDAANN+ARG YSIGK+ + +V    +K AE CD++  FLI HS GGGTGSGFT++L+E LS +Y K+ K++  V+PSP +ST+VVEPYN LL  H+ M++SD     +NE+++ +C+++L  D   + NLNRL+AQ++S TTASLRF G MN ++ +FQTNLVP+PRIHFPL ++ P +   +          ++   F   N M   DP+ G +M+CC+L+RGD+     N A++ +K K  + +VDWCPTGFKIGINY+    VP   L  + R+  +L NTTA      N++ K  ++F ++AF+HWYV EG+EE EF+EA E++  LE+D+E +
Sbjct:   86 IDDVRNGCMRELYHPEQLISGKEDAANNYARGRYSIGKEVIDRVTSRLQKIAEQCDSLQGFLIFHSLGGGTGSGFTSLLVERLSTDYSKKCKLDFAVYPSPKVSTAVVEPYNALLTTHSTMDHSDCVFMVDNEAIYDICNNSLGVDRPAYRNLNRLIAQIVSSTTASLRFSGSMNVDLNEFQTNLVPFPRIHFPLVAYAPLMSAERSAHEQHAITTLTNACFESSNMMVKCDPRAGKFMACCMLYRGDVVPKDVNAAVSAIKSKRHIQFVDWCPTGFKIGINYEKPAFVPDGDLAKTSRACCMLSNTTAISVAFSNLSYKFDLMFKKRAFVHWYVGEGMEEGEFTEARENIAVLERDFEEV 446          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: CG7794 (gene:FBgn0038565 transcript:FBtr0083540)

HSP 1 Score: 273.863 bits (699), Expect = 1.723e-85
Identity = 150/359 (41.78%), Postives = 223/359 (62.12%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKR-NKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDY 1153
            IDEI+ G YRNLF+PD+LI   +D+ +NFARG+  +  + L + +   R+ A+ C N+  FL+  + GGG+GSG    +ME L  ++GK+   +E  V+PSP++S  +VEPYN LLA H  M+ +D++   +NE+L+ +C + L+    T+TNLNR++AQ++S  TAS RF G    +  + QTNLVPYPRIH+PL ++ P V ++     N++T +++ Q F   NQM   +P  G YM+  LL+RGDI     N A+ N+K+  S  +VDW PTGFKIG++  P   VPG  L  + R+   + N T  R     +  K   L+ R+AF++ YV EGLEE  F+EA E++  L  DY
Sbjct:   75 IDEIRTGSYRNLFHPDTLITGKDDSGSNFARGYNLMASELLDRSMNAIRRVADRCRNLRGFLVFRAIGGGSGSGLGTRIMERLVEDFGKKMTVVEFLVYPSPSISPVIVEPYNALLAAHFSMDCADVSFIVDNEALYDICANTLNVPAPTYTNLNRIIAQVVSSFTASQRFGGGSTVSFQELQTNLVPYPRIHYPLINYAPLVPISHSQFVNMSTAQLTGQCFQMSNQMVRCNPSHGKYMASVLLYRGDIAPNEINTALENIKRNRSFRFVDWSPTGFKIGVSPMPPYYVPGGDLAPTNRACVAISNNTNIRIAWCRLVNKFDKLYQRRAFVYHYVGEGLEEGNFNEASENICQLVHDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: si:ch211-114n24.6 (si:ch211-114n24.6 [Source:ZFIN;Acc:ZDB-GENE-120214-26])

HSP 1 Score: 377.867 bits (969), Expect = 1.137e-125
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba8l4 (tubulin, alpha 8 like 4 [Source:ZFIN;Acc:ZDB-GENE-040426-860])

HSP 1 Score: 377.867 bits (969), Expect = 1.295e-125
Identity = 187/361 (51.80%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba4l (tubulin, alpha 4 like [Source:ZFIN;Acc:ZDB-GENE-030131-5588])

HSP 1 Score: 377.481 bits (968), Expect = 1.772e-125
Identity = 187/361 (51.80%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DEI+ G YR L++P+ LI+  EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD +   +NE++F +C  NLD +  ++TNLNRL+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI N+K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +  G ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   74 VDEIRNGHYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDSVLDRMRKMADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIFDICKRNLDIERPSYTNLNRLIAQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVPEITNACFEPANQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIANIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWGRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 434          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba4l (tubulin, alpha 4 like [Source:ZFIN;Acc:ZDB-GENE-030131-5588])

HSP 1 Score: 376.711 bits (966), Expect = 3.312e-125
Identity = 187/361 (51.80%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DEI+ G YR L++P+ LI+  EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD +   +NE++F +C  NLD +  ++TNLNRL+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI N+K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +  G ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   81 VDEIRNGHYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDSVLDRMRKMADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIFDICKRNLDIERPSYTNLNRLIAQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVPEITNACFEPANQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIANIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWGRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 441          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba8l3 (tubulin, alpha 8 like 3 [Source:ZFIN;Acc:ZDB-GENE-040801-77])

HSP 1 Score: 376.711 bits (966), Expect = 3.577e-125
Identity = 185/361 (51.25%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRKNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPSNQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tubal3.1 (tubulin, alpha 4 [Source:NCBI gene;Acc:407878])

HSP 1 Score: 378.252 bits (970), Expect = 1.018e-125
Identity = 185/361 (51.25%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR+LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C+ NLD +  ++TNLNRL+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  ++++++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGPYRSLFHPEQLITGKEDAANNYARGHYTIGKELIDSVLDRVRKMADQCSGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPRISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICNRNLDIERPSYTNLNRLIAQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVPEITNACFEYSNQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIAAIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDNKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: faah.3 (fatty acid amide hydrolase, gene 3 [Source:Xenbase;Acc:XB-GENE-997956])

HSP 1 Score: 377.867 bits (969), Expect = 1.503e-125
Identity = 186/361 (51.52%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP  AVPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   76 IDEVRNGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLERVRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVSEITNACFEPSNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 436          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba3e (tubulin alpha 3e [Source:NCBI gene;Acc:548558])

HSP 1 Score: 377.867 bits (969), Expect = 1.926e-125
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            I EI+ G YR+LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C+ NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  ++++++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   78 IGEIRTGHYRSLFHPEQLITGKEDAANNYARGHYTIGKEIVDSVLDRVRKMADQCSGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICNRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVPEITNACFEYSNQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIAAIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 438          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba3e (tubulin alpha 3e [Source:NCBI gene;Acc:548558])

HSP 1 Score: 377.481 bits (968), Expect = 1.927e-125
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            I EI+ G YR+LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C+ NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  ++++++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   74 IGEIRTGHYRSLFHPEQLITGKEDAANNYARGHYTIGKEIVDSVLDRVRKMADQCSGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICNRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVPEITNACFEYSNQMVKCDPRRGKYMACCLLYRGDVVPKDVNAAIAAIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 434          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: slc2a10 (solute carrier family 2 (facilitated glucose transporter), member 10 [Source:Xenbase;Acc:XB-GENE-950490])

HSP 1 Score: 377.481 bits (968), Expect = 2.635e-125
Identity = 185/361 (51.25%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP  AVPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   78 IDEVRAGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVSEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 438          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1c (tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:1095409])

HSP 1 Score: 376.326 bits (965), Expect = 4.689e-125
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1b (tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:107804])

HSP 1 Score: 375.555 bits (963), Expect = 8.493e-125
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1a (tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869])

HSP 1 Score: 374.785 bits (961), Expect = 2.246e-124
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3a (tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:1095406])

HSP 1 Score: 374.4 bits (960), Expect = 3.106e-124
Identity = 183/361 (50.69%), Postives = 254/361 (70.36%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF ++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3b (tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:1095408])

HSP 1 Score: 374.4 bits (960), Expect = 3.106e-124
Identity = 183/361 (50.69%), Postives = 254/361 (70.36%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF ++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q8WQ47|TBA_LEPDS (Tubulin alpha chain OS=Lepidoglyphus destructor OX=36936 PE=1 SV=2)

HSP 1 Score: 379.022 bits (972), Expect = 2.559e-125
Identity = 188/361 (52.08%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISSEKAYHEQLTVSEITNTCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q52PV9|TBA_TYRPU (Tubulin alpha chain OS=Tyrophagus putrescentiae OX=59818 PE=1 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 5.946e-125
Identity = 187/361 (51.80%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK ++ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N  + +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F  +NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVLDRIRKLSDQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVELTEFQTNLVPYPRIHFPLVTYSPVISAEKAYHEQLTVAEITNTCFEPQNQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAGIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q94572|TBA3_HOMAM (Tubulin alpha-3 chain OS=Homarus americanus OX=6706 PE=2 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 6.917e-125
Identity = 188/361 (52.08%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+NC  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P ++T+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP  AVPG  L    R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EF+EA EDL  LEKDYE +
Sbjct:   75 IDEIRTGVYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDIVLDRIRKLADNCAGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVATAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVSEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVSRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|Q91060|TBA_NOTVI (Tubulin alpha chain OS=Notophthalmus viridescens OX=8316 PE=2 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 6.918e-125
Identity = 185/361 (51.25%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI+  EDAANN+ARG Y+IGK+ + +V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP  AVPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDQVLDRMRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P06605|TBA3_DROME (Tubulin alpha-3 chain OS=Drosophila melanogaster OX=7227 GN=alphaTub84D PE=2 SV=1)

HSP 1 Score: 377.867 bits (969), Expect = 7.876e-125
Identity = 186/361 (51.52%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM  VDP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKVDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A1C8EGD5 (Tubulin alpha chain (Fragment) OS=Salpingoeca urceolata OX=473813 GN=tubA PE=2 SV=1)

HSP 1 Score: 382.874 bits (982), Expect = 2.385e-124
Identity = 187/361 (51.80%), Postives = 258/361 (71.47%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI+  EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGF ++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN +L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+  VF   +QM   DP+ G YM+CCLL+RGD+     N++I  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIVDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNAILTCHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIAQVVSSVTASLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNSVFEPASQMVKCDPRHGKYMACCLLYRGDVVPKDVNSSIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A0H5R3M8 (Tubulin alpha chain OS=Spongospora subterranea OX=70186 PE=3 SV=1)

HSP 1 Score: 382.489 bits (981), Expect = 3.922e-124
Identity = 188/361 (52.08%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI+  EDAANN+ARG Y+IGK+ +  V+   RK A+NC  +  F+I H+FGGGTGSGF ++L+E LSV+YGK++KIE  V+PSP +ST+VVEPYN++L  HA ME+SD+A   +NE+L+ +C  NL  +  T+TNLNRL+AQ IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL +  P +         L+  EI+  VF   NQM   DP+ G YM+CC+++RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +RSV ++ NTTA  +V+G ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  +EKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEVIDLVLDRLRKLADNCTGLQGFMIFHAFGGGTGSGFGSLLLERLSVDYGKKSKIEFAVYPSPQVSTAVVEPYNSVLCAHAMMEHSDVAFMVDNEALYDICRRNLGIERPTYTNLNRLIAQCISSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLCALAPVISAEIAYHEQLSVAEITNSVFEPANQMVKCDPRHGKYMACCMMYRGDVVPKDVNAAIGTVKTKRTIRFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRSVLMISNTTAIAEVMGRMDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAMEKDYEEV 435          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A0G4IPH7 (Tubulin alpha chain OS=Plasmodiophora brassicae OX=37360 GN=PBRA_005700 PE=3 SV=1)

HSP 1 Score: 382.489 bits (981), Expect = 3.922e-124
Identity = 188/361 (52.08%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI+  EDAANN+ARG Y+IGK+ +  V+   RK A+NC  +  F+I H+FGGGTGSGF ++L+E LSV+YGK++KIE  V+PSP +ST+VVEPYN++L  HA ME+SD+A   +NE+L+ +C  NL  +  T+TNLNRL+AQ IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL +  P +         L+  EI+  VF   NQM   DP+ G YM+CC+++RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +RSV ++ NTTA  +V+G ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  +EKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEVIDLVLDRLRKLADNCTGLQGFMIFHAFGGGTGSGFGSLLLERLSVDYGKKSKIEFAVYPSPQVSTAVVEPYNSVLCAHAMMEHSDVAFMVDNEALYDICRRNLGIERPTYTNLNRLIAQCISSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLCALAPVISAEIAYHEQLSVAEITNSVFEPANQMVKCDPRHGKYMACCMMYRGDVVPKDVNAAIGTVKTKRTIRFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRSVLMISNTTAIAEVMGRMDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAMEKDYEEV 435          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A0L7QKT1 (Tubulin alpha chain OS=Habropoda laboriosa OX=597456 GN=WH47_11207 PE=3 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 3.930e-124
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G Y++LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF ++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP  AVPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   41 VDEVRSGTYKHLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVIDRVRKIADQCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPRISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPIISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 401          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A507C4B1 (Tubulin alpha chain OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g04115 PE=3 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 5.009e-124
Identity = 187/361 (51.80%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+NC  +  FL+ HSFGGGTGSGF A+L+E LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   51 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADNCTGLQGFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIAQVVSSITASLRFDGSLNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLTVAEITYSCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 411          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000042562.1 (tubulin alpha chain [Source:NCBI gene;Acc:103038315])

HSP 1 Score: 378.252 bits (970), Expect = 6.822e-126
Identity = 187/361 (51.80%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEIRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRKNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPSNQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: si:ch211-114n24.6 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:103034569])

HSP 1 Score: 377.481 bits (968), Expect = 1.277e-125
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000049713.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:103046666])

HSP 1 Score: 375.555 bits (963), Expect = 7.675e-125
Identity = 185/361 (51.25%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 374.785 bits (961), Expect = 1.745e-124
Identity = 184/361 (50.97%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000008906.2 (pep primary_assembly:Astyanax_mexicanus-2.0:22:14648029:14653607:-1 gene:ENSAMXG00000008652.2 transcript:ENSAMXT00000008906.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 374.015 bits (959), Expect = 2.776e-124
Identity = 184/364 (50.55%), Postives = 256/364 (70.33%), Query Frame = 2
Query:   71 YYFIDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            ++ +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK  + C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN +L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 FFHLDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLTDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 438          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 282.337 bits (721), Expect = 9.774e-91
Identity = 141/277 (50.90%), Postives = 197/277 (71.12%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGF 910
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CC+L+RGD+     N AI  +K K ++ +VDWCPTGF
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 351          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 225.328 bits (573), Expect = 9.036e-69
Identity = 120/260 (46.15%), Postives = 172/260 (66.15%), Query Frame = 2
Query:  131 LINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGF 910
            ++   E  ++   RG    G+K   +V+   R  +E C  +  FL+ HSFGGGTGSGFT++LME LS +YGK++K+E  ++P+P +ST+VVEPYN +L  H  +E+SD +   +NE+++ +C   LD D  ++ NLNRL+AQM+S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGDI     N AI  +K + ++ +VDWCPTGF
Sbjct:   97 MLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCPTGF 356          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 178.333 bits (451), Expect = 7.364e-51
Identity = 107/331 (32.33%), Postives = 183/331 (55.29%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKR---NKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRK 1063
            +D ++ G +  +F PD+ +     A NN+A+G Y+ G + +  V+ + RK AE+CD +  F + HS GGGTGSG   +L+  +  EY  +     +  G  PSP +S +VVEPYN  L++H  +EN+D     +NE+L+ +C   L     T+ +LN L++  +SG T  LRF G +NA++     N+VP+PR+HF +  F P           LT  E+++Q+F  +N M++ DP+ G Y++   +FRG +++   +  + N++ K S  +V+W P   K  +       +P  GL   + S + + N+TA +++   I+ +   +F RK
Sbjct:   55 MDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASG--PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAV-----CDIPPRGL---KMSATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001368.1 (pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-1 gene:ENSPMAG00000001230.1 transcript:ENSPMAT00000001368.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 152.525 bits (384), Expect = 1.420e-40
Identity = 110/385 (28.57%), Postives = 185/385 (48.05%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLC----------------------HDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYV-AEGLEEIEFSEAIEDLLTLEKDYEMIE 1165
            ++E+  G  R++F+   LI     + NN+A G    G +  +K++   R TAE+CD++  F + HS GGGTGSG    ++  L  E+   ++    ++PS      V  PYN++LA+    E++D     EN++L  +                       H  L   E  F  +N ++A ++   T+S RFEG +N ++ +   NLVP+PR+H+ + S  P   +  +        ++    F KENQ+  VDP+   Y++C L+ RG++ +      I  LK   S+ +VDW   G+K G+   P +  P S L         L N T  +    +I  +   L+ RKA +H Y   EG+E   F+EA   L ++ ++Y  ++
Sbjct:   85 VNELLQGPLRDVFDQKQLITDVSGSGNNWAVGNKVYGAQFREKILERLRVTAEHCDSLQCFFLIHSMGGGTGSGLGTFVLRLLEDEFPDVHRFVMALYPSAE-DDVVTSPYNSVLAMRELTEHADCVLPIENQALVDIVNKINHAAALGKSVRSQTTLSSSHGCLKGQEKPFDTMNNIVANLMLNMTSSSRFEGSLNVDLNEITMNLVPFPRLHYLVSSLTPLYALADVALPPRRLDQMFSDAFSKENQLLRVDPRRSLYLACALMVRGNVQLSDVRRNIERLKP--SLHFVDWNEEGWKTGLCSVPPVGYPHSLLA--------LANNTCVKHTFADIQERFARLYRRKAHLHHYTDVEGMELSCFTEAASSLASVIEEYNQLD 458          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 93.9745 bits (232), Expect = 7.874e-21
Identity = 81/354 (22.88%), Postives = 169/354 (47.74%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYED--AANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPT-LSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTK-EISEQVFLKENQMSSVDPKEGA---YMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQ-PMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSE 1117
            I+ I    Y NL+NP+++  S     A NN+A G +S G+K  +++  +  + A+  D++    +         +     L +Y    + K+      VFP+   +S  VV+PYN+LL L    +N+D     +N +L R+  D L     +F+ +N+L++ ++S +T +LR+ G MN ++I    +L+P PR+HF +  + P      + S   TT  ++  ++   +N M S          Y++   + +G+++    + ++  ++++   +++ W P   ++ ++ + P L          R S  ++ N T+   +     ++   L  R+AF+  +  E + + + SE
Sbjct:   74 INSIVNSSYANLYNPENIYLSEHGGGAGNNWASG-HSQGEKIHEEIFDIIDREADGSDSLEKCPLTRRLSATNWNE----LCKYF-CRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLT------SAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSE 415          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 341.658 bits (875), Expect = 1.189e-112
Identity = 172/358 (48.04%), Postives = 243/358 (67.88%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDY 1153
            IDE++ G+++ LF+P+ LIN  EDAANN+ARG Y++G++ + +V    RK A+ CD +  FL  HS GGGTGSG  ++L+E LS EYGK++K+E  V+P+P LSTSVVEPYNT+L  H  +E++D     +NE+++ +C  NL     +F+NLN L+AQ+IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +   +    + +  EI+   F   NQM   DP +G YM+ CLL+RGD+       A+  +K K ++  VDWCPTGFKIGI Y+P   +P S L    R+V +L NTTA       I++K  +++ ++AF+HWYV EG+EE EF+EA EDL  LE+DY
Sbjct:   76 IDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 340.887 bits (873), Expect = 2.339e-112
Identity = 174/358 (48.60%), Postives = 246/358 (68.72%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDY 1153
            IDE++ G Y++LF+P+ LI+  EDAANN+ARG Y++G++ L  V+   RK A+ CD +  FL  HS GGGTGSG  ++L+E LS EYGK++K+E  V+P+P +STSVVEPYNT+L  H  +E++D     +NE+++ +C  NLD    +F NLN L+AQ++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +  +K    + +  EI+   F   NQM   DP++G YM+ CLL+RGD+       A+  +K K ++  VDWCPTGFKIGI Y+P  A P S L    R+V +L NTT+  +    I+RK  +++ ++AF+HWYV EG+EE EF+EA EDL  LE+DY
Sbjct:   76 IDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 214.542 bits (545), Expect = 1.016e-63
Identity = 117/362 (32.32%), Postives = 207/362 (57.18%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIE 1165
            ID ++     NLF PD+ I     A N +A+G Y+ G + +  V+ + R+ AE CD++  F I HS GGGTGSG   +L+  +  E+  R      V PSP  S +VVEPYN  L++H  +E+SD     +NE+L+ +C   L  ++ ++ +LN L++ ++SG T SLR+ G +N+++     NLVP+PR+HF +  + P   +      +LT  E+++Q+F  +N M++ DP+ G Y++    FRG +++    + ++ ++ K S  +V+W P   +  +      +V   GL     + + + N+T+ +++   +  +   +F RKAF+HWY +EG++E+EFSEA  ++  L  +Y+  +
Sbjct:   73 IDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAV-----CSVAPQGLDM---AATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEYQQYQ 426          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 81.2629 bits (199), Expect = 8.989e-17
Identity = 65/196 (33.16%), Postives = 101/196 (51.53%), Query Frame = 2
Query:  104 YRNLFNPDS--LINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFV 685
            +R  F+P +  + +    A N++A G Y IG +    ++    K  ++ DN   F + HS  GGTGSG  + L+E L   Y K+      VFP+ + S  VV+ YNT+LAL   +E+SD    F+N SL  +         I   + N+L++ +IS  T S+RF   M +++    + L+P P +HF   SF PF 
Sbjct:   83 FRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPARS-SEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFT 276          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO46332 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQB6])

HSP 1 Score: 381.333 bits (978), Expect = 1.532e-127
Identity = 185/361 (51.25%), Postives = 259/361 (71.75%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G YR LF+P+ ++   EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGF+++LME LSV+YGKR+K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +G +K    N++  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 IDEIRTGTYRQLFHPEQMVTGKEDAANNYARGHYTVGKELIDVVLDKIRKLADQCTGLQGFLVFHSFGGGTGSGFSSLLMERLSVDYGKRSKLEFAVYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVIGADKAYHENMSVAEITSACFEPSNQMVKCDPRYGKYMACCLLYRGDVVPKDVNSAIAAIKMKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO44766 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUX0])

HSP 1 Score: 379.022 bits (972), Expect = 3.347e-127
Identity = 185/361 (51.25%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE+++G YR+LF+PD LI   EDAANN+ARG Y++GK+ +   +   RK A+ C  +  FLI HSFGGGTGSGF+++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQ   AVPG  L   +RSV +L NTTA       +N K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   40 VDEVRMGTYRDLFHPDQLITGKEDAANNYARGHYTVGKEHIDLTLEKIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQQPTAVPGGDLAKVQRSVCMLSNTTAIADAWARLNHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 400          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO40781 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6A0])

HSP 1 Score: 378.252 bits (970), Expect = 1.374e-126
Identity = 187/361 (51.80%), Postives = 254/361 (70.36%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEI+ G YR LF+P+ LI   EDAANN+ARG Y++GK  +  V+   RK A+ C  +  FLI HSFGGGTGSGF+++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   60 IDEIRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKDHIDNVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAVYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNSCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 420          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO44557 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVD9])

HSP 1 Score: 378.252 bits (970), Expect = 1.466e-126
Identity = 184/361 (50.97%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF ++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD D  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N++I  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   60 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRCRKLADQCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIDRPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSSIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 420          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO40780 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S699])

HSP 1 Score: 378.252 bits (970), Expect = 2.686e-126
Identity = 185/361 (51.25%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF+++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     LT  EI+   F   NQM   DP+ G YM+CCLLFRGD+     N AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   75 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEMIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITTSCFEPANQMVKCDPRHGKYMACCLLFRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020157.2 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:101166346])

HSP 1 Score: 377.867 bits (969), Expect = 3.261e-126
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA EDL  LEKDYE +
Sbjct:   40 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNTCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 400          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020049.2 (tubulin alpha-1B chain [Source:NCBI gene;Acc:101159587])

HSP 1 Score: 376.711 bits (966), Expect = 9.633e-126
Identity = 185/361 (51.25%), Postives = 256/361 (70.91%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSGFT++LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   40 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVKRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 400          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000003572.2 (tubulin alpha chain [Source:NCBI gene;Acc:101171518])

HSP 1 Score: 377.096 bits (967), Expect = 1.509e-125
Identity = 185/361 (51.25%), Postives = 258/361 (71.47%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y++GK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI ++K + S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIASIKTRRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: tuba8l3 (tubulin alpha chain [Source:NCBI gene;Acc:101168255])

HSP 1 Score: 376.711 bits (966), Expect = 2.733e-125
Identity = 186/361 (51.52%), Postives = 257/361 (71.19%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  +I+   F   NQM   DP+ G YM+CCLL+RGD+     N+AI  +K K ++ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:   75 IDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 435          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020468.2 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:101155224])

HSP 1 Score: 377.481 bits (968), Expect = 4.944e-125
Identity = 186/361 (51.52%), Postives = 255/361 (70.64%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            +DE++ G YR LF+P+ LI   EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGFT++LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ LC  NLD +  ++TNLNRL+ Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K     L+  EI+   F   NQM   DP+ G YM+CCLL+RGD+     N AI  +K K S+ +VDWCPTGFK+GINYQP   VPG  L   +R+V +L NTTA  +    ++ K  +++ ++AF+HWYV EG+EE EFSEA ED+  LEKDYE +
Sbjct:  117 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDLCRRNLDIERPSYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKEVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV 477          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000017516.1 (SMESG000017516.1)

HSP 1 Score: 899.427 bits (2323), Expect = 0.000e+0
Identity = 479/507 (94.48%), Postives = 483/507 (95.27%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIEXXXXXXXXXXXXXXXXXXXNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE 1600
            IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGA  +   L +                 KWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLG+IN+KCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIETTTETQSTS FTSTRFGSSNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE
Sbjct:   29 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGANNAINNLKK-----------------KWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGSINKKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIETTTETQSTSIFTSTRFGSSNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE 518          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000017516.1 (SMESG000017516.1)

HSP 1 Score: 806.594 bits (2082), Expect = 0.000e+0
Identity = 433/461 (93.93%), Postives = 437/461 (94.79%), Query Frame = 2
Query:  218 MTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIEXXXXXXXXXXXXXXXXXXXNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE 1600
            MTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGA  +   L +                 KWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLG+IN+KCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIETTTETQSTS FTSTRFGSSNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE
Sbjct:    1 MTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGANNAINNLKK-----------------KWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGSINKKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIETTTETQSTSIFTSTRFGSSNKRSANSENSTPEITVETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLYQCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPFQNEATEYFVTE 444          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000017516.1 (SMESG000017516.1)

HSP 1 Score: 654.825 bits (1688), Expect = 0.000e+0
Identity = 342/361 (94.74%), Postives = 344/361 (95.29%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 1162
            IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGA                 NNAINNLKKKWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLG+IN+KCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI
Sbjct:   29 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGA-----------------NNAINNLKKKWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGSINKKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMI 372          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000037910.1 (SMESG000037910.1)

HSP 1 Score: 434.876 bits (1117), Expect = 3.520e-147
Identity = 215/362 (59.39%), Postives = 278/362 (76.80%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIE 1165
            +DEIK+G+YR LF+P++LI+  EDA+NNF+RGFYS GK A+++V+  TRK  ENCD + AF+ +HSFGGGTGSGFT+ML+EYL+VEY KR K+E GVFPSPTLS +VVEPYN +LALH PMEN+D+A FFENESL                    + AQ+ISG T S+RFEGP+N NIID+QTNLVPYPRIHFPL SF PF+  +K+   +++T+EI+   F KENQM+  DP  G YMSCC+L+RGD+NI   N+AI   K   +  WVDWCPTG+KIGINYQPM+AVPGSG+ +S RS++LLCNTTA ++V+  +N K ++++  K+F HWY+AEG+EE EF EA ED+L LEKD+E+IE
Sbjct:   55 LDEIKVGRYRQLFHPENLISRNEDASNNFSRGFYSCGKLAIKEVIRNTRKLVENCDEVQAFMAHHSFGGGTGSGFTSMLLEYLAVEYSKRTKVEFGVFPSPTLSVAVVEPYNAVLALHTPMENADVAVFFENESL--------------------MAAQVISGMTCSMRFEGPINTNIIDYQTNLVPYPRIHFPLVSFSPFLAQSKLSHYSMSTREITFDAFPKENQMAETDPSRGIYMSCCMLYRGDVNIREVNDAIGYQKSVKNQQWVDWCPTGYKIGINYQPMVAVPGSGIAYSSRSLTLLCNTTAVKQVVSRLNHKFMLMYMHKSFFHWYIAEGMEEDEFKEAFEDVLMLEKDFEVIE 396          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000037910.1 (SMESG000037910.1)

HSP 1 Score: 434.106 bits (1115), Expect = 1.545e-146
Identity = 215/362 (59.39%), Postives = 278/362 (76.80%), Query Frame = 2
Query:   80 IDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXAMLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFRGDXXXXXXXXXXXXXXXXWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGFSRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSEAIEDLLTLEKDYEMIE 1165
            +DEIK+G+YR LF+P++LI+  EDA+NNF+RGFYS GK A+++V+  TRK  ENCD + AF+ +HSFGGGTGSGFT+ML+EYL+VEY KR K+E GVFPSPTLS +VVEPYN +LALH PMEN+D+A FFENESL                    + AQ+ISG T S+RFEGP+N NIID+QTNLVPYPRIHFPL SF PF+  +K+   +++T+EI+   F KENQM+  DP  G YMSCC+L+RGD+NI   N+AI   K   +  WVDWCPTG+KIGINYQPM+AVPGSG+ +S RS++LLCNTTA ++V+  +N K ++++  K+F HWY+AEG+EE EF EA ED+L LEKD+E+IE
Sbjct:   74 LDEIKVGRYRQLFHPENLISRNEDASNNFSRGFYSCGKLAIKEVIRNTRKLVENCDEVQAFMAHHSFGGGTGSGFTSMLLEYLAVEYSKRTKVEFGVFPSPTLSVAVVEPYNAVLALHTPMENADVAVFFENESL--------------------MAAQVISGMTCSMRFEGPINTNIIDYQTNLVPYPRIHFPLVSFSPFLAQSKLSHYSMSTREITFDAFPKENQMAETDPSRGIYMSCCMLYRGDVNIREVNDAIGYQKSVKNQQWVDWCPTGYKIGINYQPMVAVPGSGIAYSSRSLTLLCNTTAVKQVVSRLNHKFMLMYMHKSFFHWYIAEGMEEDEFKEAFEDVLMLEKDFEVIE 415          
The following BLAST results are available for this feature:
BLAST of Tubulin alpha chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA1A5.973e-12550.97tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1C9.970e-12551.25tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1B1.237e-12451.25tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:1880... [more]
TUBA1A3.271e-12450.97tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1A3.271e-12450.97tubulin alpha 1a [Source:HGNC Symbol;Acc:HGNC:2076... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tba-91.346e-12551.80Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
mec-126.579e-12351.25Tubulin alpha-3 chain Detyrosinated tubulin alpha-... [more]
tba-42.542e-11949.31Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
tba-23.708e-11948.75Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Pro... [more]
tba-23.708e-11948.75Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Pro... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alphaTub84D7.857e-12651.52gene:FBgn0003885 transcript:FBtr0081538[more]
alphaTub84B7.766e-12550.97gene:FBgn0003884 transcript:FBtr0081639[more]
alphaTub85E2.416e-12450.69gene:FBgn0003886 transcript:FBtr0082087[more]
alphaTub67C1.055e-10846.26gene:FBgn0087040 transcript:FBtr0076393[more]
CG77941.723e-8541.78gene:FBgn0038565 transcript:FBtr0083540[more]
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BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-114n24.61.137e-12551.52si:ch211-114n24.6 [Source:ZFIN;Acc:ZDB-GENE-120214... [more]
tuba8l41.295e-12551.80tubulin, alpha 8 like 4 [Source:ZFIN;Acc:ZDB-GENE-... [more]
tuba4l1.772e-12551.80tubulin, alpha 4 like [Source:ZFIN;Acc:ZDB-GENE-03... [more]
tuba4l3.312e-12551.80tubulin, alpha 4 like [Source:ZFIN;Acc:ZDB-GENE-03... [more]
tuba8l33.577e-12551.25tubulin, alpha 8 like 3 [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubal3.11.018e-12551.25tubulin, alpha 4 [Source:NCBI gene;Acc:407878][more]
faah.31.503e-12551.52fatty acid amide hydrolase, gene 3 [Source:Xenbase... [more]
tuba3e1.926e-12551.52tubulin alpha 3e [Source:NCBI gene;Acc:548558][more]
tuba3e1.927e-12551.52tubulin alpha 3e [Source:NCBI gene;Acc:548558][more]
slc2a102.635e-12551.25solute carrier family 2 (facilitated glucose trans... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tuba1c4.689e-12551.25tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba1b8.493e-12551.25tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:10780... [more]
Tuba1a2.246e-12450.97tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869... [more]
Tuba3a3.106e-12450.69tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba3b3.106e-12450.69tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:10954... [more]
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BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8WQ47|TBA_LEPDS2.559e-12552.08Tubulin alpha chain OS=Lepidoglyphus destructor OX... [more]
sp|Q52PV9|TBA_TYRPU5.946e-12551.80Tubulin alpha chain OS=Tyrophagus putrescentiae OX... [more]
sp|Q94572|TBA3_HOMAM6.917e-12552.08Tubulin alpha-3 chain OS=Homarus americanus OX=670... [more]
sp|Q91060|TBA_NOTVI6.918e-12551.25Tubulin alpha chain OS=Notophthalmus viridescens O... [more]
sp|P06605|TBA3_DROME7.876e-12551.52Tubulin alpha-3 chain OS=Drosophila melanogaster O... [more]
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BLAST of Tubulin alpha chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1C8EGD52.385e-12451.80Tubulin alpha chain (Fragment) OS=Salpingoeca urce... [more]
A0A0H5R3M83.922e-12452.08Tubulin alpha chain OS=Spongospora subterranea OX=... [more]
A0A0G4IPH73.922e-12452.08Tubulin alpha chain OS=Plasmodiophora brassicae OX... [more]
A0A0L7QKT13.930e-12451.25Tubulin alpha chain OS=Habropoda laboriosa OX=5974... [more]
A0A507C4B15.009e-12451.80Tubulin alpha chain OS=Synchytrium microbalum OX=1... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000042562.16.822e-12651.80tubulin alpha chain [Source:NCBI gene;Acc:10303831... [more]
si:ch211-114n24.61.277e-12551.52tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000049713.17.675e-12551.25tubulin alpha-1A chain [Source:NCBI gene;Acc:10304... [more]
ENSAMXT00000030346.11.745e-12450.97tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
ENSAMXT00000008906.22.776e-12450.55pep primary_assembly:Astyanax_mexicanus-2.0:22:146... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA3C9.774e-9150.90tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000001139.19.036e-6946.15pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
tubb27.364e-5132.33tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
ENSPMAT00000001368.11.420e-4028.57pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-... [more]
tubg17.874e-2122.88tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB31.189e-11248.04Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB12.339e-11248.60Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB21.016e-6332.32Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB48.989e-1733.16Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO463321.532e-12751.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447663.347e-12751.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407811.374e-12651.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445571.466e-12650.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407802.686e-12651.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000020157.23.261e-12651.52tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
ENSORLT00000020049.29.633e-12651.25tubulin alpha-1B chain [Source:NCBI gene;Acc:10115... [more]
ENSORLT00000003572.21.509e-12551.25tubulin alpha chain [Source:NCBI gene;Acc:10117151... [more]
tuba8l32.733e-12551.52tubulin alpha chain [Source:NCBI gene;Acc:10116825... [more]
ENSORLT00000020468.24.944e-12551.52tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
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BLAST of Tubulin alpha chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017516.10.000e+094.48SMESG000017516.1[more]
SMESG000017516.10.000e+093.93SMESG000017516.1[more]
SMESG000017516.10.000e+094.74SMESG000017516.1[more]
SMESG000037910.13.520e-14759.39SMESG000037910.1[more]
SMESG000037910.11.545e-14659.39SMESG000037910.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006461 ID=SMED30006461|Name=Tubulin alpha chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1739bp
TTTGTGAGGAAGTTAAGGAGTTTAAATGAAATTATTTTTCCTAGATTAAC
TTAATGAATAAATATAAGTTTATTATTTTATAGATGAAATAAAGCTTGGG
AAATATAGAAATTTATTCAATCCTGATTCCTTGATTAACTCATATGAAGA
TGCAGCAAACAATTTTGCTAGAGGATTTTACTCTATTGGTAAAAAGGCAT
TACAAAAAGTGGTTTATATGACTCGCAAAACTGCTGAAAACTGTGATAAT
ATTCATGCATTTCTGATAAATCATTCATTCGGTGGTGGTACCGGTTCTGG
CTTCACTGCAATGTTAATGGAATATCTATCTGTGGAGTATGGAAAAAGGA
ATAAAATTGAATGTGGAGTATTCCCAAGCCCTACACTTAGTACTTCAGTG
GTGGAGCCGTACAATACCTTATTAGCATTGCATGCACCAATGGAAAATTC
GGATCTCGCCGCTTTTTTTGAAAATGAATCATTGTTTCGACTCTGCCACG
ATAATTTAGACTTCGATGAAATCACTTTCACAAATTTAAATCGCCTTCTC
GCACAAATGATAAGTGGGACGACTGCATCACTAAGATTTGAAGGTCCTAT
GAATGCAAACATAATCGACTTCCAAACAAATCTGGTACCATATCCAAGAA
TACATTTCCCATTATTCAGTTTTTATCCATTCGTAGGAATGAATAAAATC
GACAGTGCTAACTTGACGACTAAAGAAATTTCTGAACAAGTTTTCCTCAA
AGAAAACCAAATGTCAAGTGTTGATCCAAAAGAAGGGGCTTACATGAGTT
GTTGTCTCTTGTTTAGAGGCGATATAAATATTTTAGGTGCAAATAATGCC
ATCAACAATTTAAAGAAAAAGTGGTCCATGTCGTGGGTTGATTGGTGTCC
GACTGGATTCAAGATCGGTATAAATTATCAACCTATGTTAGCTGTTCCCG
GCAGCGGTTTGGGATTTAGTAGAAGATCTGTATCTCTATTATGTAATACA
ACGGCATTTCGAAAAGTGCTTGGTAACATTAATAGGAAATGTTTAATTCT
GTTCAATCGAAAAGCATTTATACATTGGTATGTGGCGGAAGGACTAGAAG
AAATCGAATTTTCTGAAGCCATAGAAGATTTGCTGACGTTGGAAAAGGAC
TACGAAATGATTGAAACGACAACAGAAACACAAAGTACATCCACATTTAC
ATCCACAAGATTTGGTTCTTCCAATAAAAGGTCCGCAAATTCTGAAAATT
CCACTCCCGAAATAACAGTTGAAACACCGAAATCGACTTCCGAATCAACT
CAGAAACCAGGTTACTGTTCTAGCACTACCTCCAATGATGATATCCTTAT
TATAAATCCACCCAATTCTGAAAATTCCAATCAAGCTTCTAAAAGGGTTT
TTGAAACTGATTTGTATCAATGTTCTGAGAAGAAGTTGAACAGAATTAAA
AACTGTTTCCAATATTCTAATCCTGTGGTTTCACCTACATCTATTTATCC
GACGAATCAAAAGAATGTTAATAATATTTTGAAAAATGCCCTTACCTTCA
AACCCCTTACACCATTCCAAAATGAAGCTACAGAATATTTTGTAACGGAA
AACAGTCTGCTAACTGTCGACACGAGTGATAATTACCAAGAAAAATTAAT
TTAATTAATTAACTTGATTTTTTTGATTTCAATCAAAATTATTTTAAATT
CTTTAATTATTTATTAGTTTGTATTATATTTCTTTCTCC
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protein sequence of SMED30006461-orf-1

>SMED30006461-orf-1 ID=SMED30006461-orf-1|Name=SMED30006461-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=479bp
MTRKTAENCDNIHAFLINHSFGGGTGSGFTAMLMEYLSVEYGKRNKIECG
VFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFD
EITFTNLNRLLAQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLF
SFYPFVGMNKIDSANLTTKEISEQVFLKENQMSSVDPKEGAYMSCCLLFR
GDINILGANNAINNLKKKWSMSWVDWCPTGFKIGINYQPMLAVPGSGLGF
SRRSVSLLCNTTAFRKVLGNINRKCLILFNRKAFIHWYVAEGLEEIEFSE
AIEDLLTLEKDYEMIETTTETQSTSTFTSTRFGSSNKRSANSENSTPEIT
VETPKSTSESTQKPGYCSSTTSNDDILIINPPNSENSNQASKRVFETDLY
QCSEKKLNRIKNCFQYSNPVVSPTSIYPTNQKNVNNILKNALTFKPLTPF
QNEATEYFVTENSLLTVDTSDNYQEKLI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR002452Alpha_tubulin
IPR000217Tubulin
IPR003008Tubulin_FtsZ_GTPase
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: molecular function
TermDefinition
GO:0005200structural constituent of cytoskeleton
GO:0005525GTP binding
GO:0003924GTPase activity
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton