hesl-1 protein

Overview
Namehesl-1 protein (AGZ94925.1)
Smed IDSMED30006440
Length (bp)728
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of hesl-1 protein (SMED30006440) t-SNE clustered cells

Violin plots show distribution of expression levels for hesl-1 protein (SMED30006440) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006440

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006440h1SMcG0017411 SMED30006440smed_20140614PMID:29100657
Cheng et al., 2018		FACS sorted cell population asexual adult RNA-sequencing evidence
central nervous systemSMED30006440h1SMcG0017411 KF487110smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30006440h1SMcG0017411 KF487110smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30006440h1SMcG0017411 KF487110smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30006440h1SMcG0017411 KF487110smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of hesl-1 protein vs. Ensembl Fly
Match: E(spl)m7-HLH (gene:FBgn0002633 transcript:FBtr0084960)

HSP 1 Score: 45.4394 bits (106), Expect = 9.957e-6
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 3 
Query:  189 KENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSN 428
            K    RK+ K  +ER+RRAR+N  LD+LK +M +   +  +   K EKA+IL+  V+       RK+   KKH   +P+N
Sbjct:    9 KTYQYRKVMKPLLERKRRARINKCLDELKDLMAEC--VAQTGDAKFEKADILEVTVQHL-----RKLKESKKH---VPAN 78
[Close]
BLAST of hesl-1 protein vs. Ensembl Zebrafish
Match: hes2.1 (hes family bHLH transcription factor 2, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-081104-104])

HSP 1 Score: 50.8322 bits (120), Expect = 1.148e-7
Identity = 40/127 (31.50%), Postives = 70/127 (55.12%), Query Frame = 3 
Query:  168 KMDTSQKKE-NSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK-IIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTD-RTKFVQR 539
            +M  +Q+KE + LRK  K  ME+RRRAR+N SL+ LK+++  +   D S   K+EKA+IL+  V F   + +     Q   +K    + +  I+T+  ++N+    +T+    ++EF   R + + R
Sbjct:   17 RMTVAQRKEAHELRKTLKPLMEKRRRARINDSLNHLKTLILPLVGKDASRYSKLEKADILEMTVRFLRDLPSSSAKGQTDSYKEGYKACLQRISTMLPQSNL----ETEAHQRVSEFIHTRWRLLHR 139
[Close]
BLAST of hesl-1 protein vs. Ensembl Zebrafish
Match: hes2.1 (hes family bHLH transcription factor 2, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-081104-104])

HSP 1 Score: 50.8322 bits (120), Expect = 1.148e-7
Identity = 40/127 (31.50%), Postives = 70/127 (55.12%), Query Frame = 3 
Query:  168 KMDTSQKKE-NSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK-IIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTD-RTKFVQR 539
            +M  +Q+KE + LRK  K  ME+RRRAR+N SL+ LK+++  +   D S   K+EKA+IL+  V F   + +     Q   +K    + +  I+T+  ++N+    +T+    ++EF   R + + R
Sbjct:   17 RMTVAQRKEAHELRKTLKPLMEKRRRARINDSLNHLKTLILPLVGKDASRYSKLEKADILEMTVRFLRDLPSSSAKGQTDSYKEGYKACLQRISTMLPQSNL----ETEAHQRVSEFIHTRWRLLHR 139
[Close]
BLAST of hesl-1 protein vs. Ensembl Zebrafish
Match: hes2.2 (hes family bHLH transcription factor 2, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-060825-55])

HSP 1 Score: 48.1358 bits (113), Expect = 1.852e-6
Identity = 25/60 (41.67%), Postives = 41/60 (68.33%), Query Frame = 3 
Query:  183 QKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEF 362
            +K+ + LRK  K  +E++RRAR+N SLD+LK+++  +   D+    K+EKA+IL+  V F
Sbjct:   21 RKEASELRKTLKPLLEKKRRARINDSLDRLKALILPLTGKDNCRYSKLEKADILEMTVRF 80
[Close]
BLAST of hesl-1 protein vs. Ensembl Xenopus
Match: drc3 (dynein regulatory complex subunit 3 [Source:Xenbase;Acc:XB-GENE-1003585])

HSP 1 Score: 51.9878 bits (123), Expect = 1.442e-7
Identity = 27/61 (44.26%), Postives = 44/61 (72.13%), Query Frame = 3 
Query:  198 SLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVN 380
            SLRK+SK  ME++RRAR+NVSL++LK +++K  +  +    K+EKA+IL+  V++   + N
Sbjct:   15 SLRKVSKPLMEKKRRARINVSLEQLKGLLEK-NYSQNIRKRKLEKADILELTVKYLKTLQN 74
[Close]
BLAST of hesl-1 protein vs. Ensembl Xenopus
Match: HES2 (hes family bHLH transcription factor 2 [Source:NCBI gene;Acc:100038090])

HSP 1 Score: 48.1358 bits (113), Expect = 2.308e-6
Identity = 29/71 (40.85%), Postives = 42/71 (59.15%), Query Frame = 3 
Query:  201 LRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK 413
            LRK  K  ME+RRRAR+N SL++LK+++  +   D+S   K+EKA+IL+  V F   I      N    +K
Sbjct:   28 LRKTLKPLMEKRRRARINESLNQLKTLILPLIGKDNSRYSKLEKADILEMTVRFLRDIPPVPAQNPADRYK 98
[Close]
BLAST of hesl-1 protein vs. Ensembl Mouse
Match: Hes3 (hes family bHLH transcription factor 3 [Source:MGI Symbol;Acc:MGI:104877])

HSP 1 Score: 52.373 bits (124), Expect = 6.750e-8
Identity = 31/92 (33.70%), Postives = 54/92 (58.70%), Query Frame = 3 
Query:  156 TDIIKMDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNI 431
            T+    D+     +S RKISK  ME++RRAR+NVSL++L+S++++  +       K+EKA+IL+  V++      R + N  +    +PS +
Sbjct:    3 TEPTSRDSLGGPASSFRKISKPLMEKKRRARINVSLEQLRSLLERH-YSHQIRKRKLEKADILELSVKYM-----RSLQNSLQGLWPVPSGV 88
[Close]
BLAST of hesl-1 protein vs. UniProt/SwissProt
Match: sp|Q00P32|HES2_XENLA (Transcription factor HES-2 OS=Xenopus laevis OX=8355 GN=hes2 PE=1 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 1.176e-6
Identity = 39/118 (33.05%), Postives = 64/118 (54.24%), Query Frame = 3 
Query:  186 KKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETNIL-----LTNQTKPTNSMAEFTDRT 524
            ++ + LRK  K  ME+RRRAR+N SL++LK+++  +   D+S   K+EKA+IL+  V F   I   +  NQ   +K      G+   V+  + IL     LT +   +N + E+  R+
Sbjct:   23 QEASELRKTLKPLMEKRRRARINESLNQLKTLILPLIGKDNSRYSKLEKADILEMTVRFLRDIPPVQAQNQADRYK-----EGYRACVERLSAILGKSHVLTGEA--SNRLLEYLQRS 133
[Close]
BLAST of hesl-1 protein vs. TrEMBL
Match: U5YTN2 (Hesl-1 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=hesl-1 PE=2 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.851e-107
Identity = 152/154 (98.70%), Postives = 153/154 (99.35%), Query Frame = 3 
Query:  222 EMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE 683
            EMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQI +RKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE
Sbjct:    1 EMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIGDRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE 154
[Close]
BLAST of hesl-1 protein vs. TrEMBL
Match: A0A444UX16 (Transcription factor HES-2 OS=Acipenser ruthenus OX=7906 GN=EOD39_19871 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 5.820e-7
Identity = 37/103 (35.92%), Postives = 63/103 (61.17%), Query Frame = 3 
Query:  168 KMDTSQKKENS-LRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK-IIPSNIGHINTVKSETNIL 470
            +M  +++KE S LRK  K  ME+RRRAR+N SL++LK+++  +   D+S   K+EKA+IL+  V F   I +  + N    F+    + + H++++   TN+L
Sbjct:   15 RMTVAKRKEASELRKTLKPLMEKRRRARINESLNQLKTLILPLIGKDNSRYSKLEKADILEMTVRFLKDIPSTPVQNAADSFREGYKACLQHVSSILPNTNLL 117
[Close]
BLAST of hesl-1 protein vs. TrEMBL
Match: A0A556U225 (Transcription factor HES-2 OS=Bagarius yarrelli OX=175774 GN=Baya_7546 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.790e-6
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 3 
Query:  168 KMDTSQKKE-NSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK 413
            +M  +Q+KE N LRK  K  ME+RRRAR+N SL+KLK ++  +   D S   K+EKA+IL+  V F  ++ +  +  Q   ++
Sbjct:   16 RMTVAQRKEANELRKTLKPLMEKRRRARINESLNKLKELIVPLAGKDSSRYSKLEKADILEMTVRFLRELPSSPVKAQADMYR 98
[Close]
BLAST of hesl-1 protein vs. TrEMBL
Match: A0A3B3SU42 (Hes family bHLH transcription factor 2 OS=Paramormyrops kingsleyae OX=1676925 GN=HES2 (1 of many) PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 8.639e-6
Identity = 31/77 (40.26%), Postives = 49/77 (63.64%), Query Frame = 3 
Query:  183 QKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK 413
            +K+ N LRK  K  ME+RRRAR+N SL++LK+++  +   D+S   K+EKA+ILD  V F   + +  + N+   +K
Sbjct:   22 RKEANELRKTLKPLMEKRRRARINESLNQLKNLIVPLIGKDNSRYSKLEKADILDMTVRFLRDLPSAPVKNRSDSYK 98
[Close]
BLAST of hesl-1 protein vs. TrEMBL
Match: A0A401RZF1 (BHLH domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0001919 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 8.939e-6
Identity = 42/138 (30.43%), Postives = 75/138 (54.35%), Query Frame = 3 
Query:  168 KMDTSQKKENS-LRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTDRTKFVQR-MPLSTLNPNQMN 575
            +++ S++KE S LRK  K  ME+RRRAR+N SL++LK+++  +   D     K+EKA+IL+  V F   +    +Y++   ++      G+   +    N+L +++   T +    TD   +++R MPL +  P   N
Sbjct:   19 QLNKSKRKEASELRKTLKPLMEKRRRARINDSLNQLKTLILPLIGKDSPRYSKLEKADILEMTVRFLQDLPKTPLYDRLDSYR-----EGYRACLLQLANLLPSSKLMGTEACDRLTD---YLERCMPLVSYYPYSHN 148
[Close]
BLAST of hesl-1 protein vs. Ensembl Cavefish
Match: ENSAMXT00000035814.1 (transcription factor HES-4-B-like [Source:NCBI gene;Acc:111194137])

HSP 1 Score: 48.1358 bits (113), Expect = 1.189e-6
Identity = 24/59 (40.68%), Postives = 38/59 (64.41%), Query Frame = 3 
Query:  180 SQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVV 356
            SQ ++N  R++SK  +ERRRRAR+N  L +L+S++ +          K+EKA+IL+  V
Sbjct:   18 SQSEKNPQRRLSKPLLERRRRARINACLGELRSLLLQSHATQGCHPSKLEKADILELTV 76
[Close]
BLAST of hesl-1 protein vs. Ensembl Cavefish
Match: hes2.1 (hes family bHLH transcription factor 2, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-081104-104])

HSP 1 Score: 46.595 bits (109), Expect = 3.568e-6
Identity = 24/63 (38.10%), Postives = 40/63 (63.49%), Query Frame = 3 
Query:  225 MERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK 413
            ME+RRRAR+N SL+KLK+++  +   D S   K+EKA++L+  V F   + +  + +Q   +K
Sbjct:   16 MEKRRRARINDSLNKLKALIVPLVGKDTSRYSKLEKADVLEMTVSFLRDLPSSPVKDQADSYK 78
[Close]
BLAST of hesl-1 protein vs. Ensembl Sea Lamprey
Match: her9 (hairy-related 9 [Source:ZFIN;Acc:ZDB-GENE-011213-1])

HSP 1 Score: 42.743 bits (99), Expect = 8.824e-6
Identity = 25/57 (43.86%), Postives = 39/57 (68.42%), Query Frame = 3 
Query:  186 KKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVV 356
            K  + +RK +K  ME+RRRAR+N SL++LK+++ +    D S   K+EKA+IL+  V
Sbjct:   11 KSASEIRKSTKPIMEKRRRARINDSLNQLKALILETLRKDSSRHSKLEKADILELTV 67
[Close]
BLAST of hesl-1 protein vs. Ensembl Nematostella
Match: EDO45031 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU73])

HSP 1 Score: 46.595 bits (109), Expect = 2.647e-6
Identity = 32/94 (34.04%), Postives = 54/94 (57.45%), Query Frame = 3 
Query:  183 QKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETN 464
            Q +E+  RK SK  ME+RRRAR+N SL++LK ++ +    D S   K+EKA+IL+  V++      R +   ++   I+PS+   +   ++  N
Sbjct:   11 QLEESVGRKSSKPMMEKRRRARINQSLNELKILILEAMKKDTSCYSKLEKADILEMTVKYL-----RAMKTTQQLTGIVPSDPSSVAQYRAGFN 99
[Close]
BLAST of hesl-1 protein vs. Ensembl Medaka
Match: HES2 (hes family bHLH transcription factor 2 [Source:NCBI gene;Acc:101167036])

HSP 1 Score: 48.521 bits (114), Expect = 1.166e-6
Identity = 27/77 (35.06%), Postives = 48/77 (62.34%), Query Frame = 3 
Query:  183 QKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFK 413
            +K+ N LRK  K  +E+RRRAR+N SL+ LKS++  +   D++   K+EKA+IL+  V F   + +  + +  + ++
Sbjct:   22 RKQANELRKTLKPLLEKRRRARINDSLNHLKSLILPLVGKDNARYSKLEKADILEMTVRFLRDLPSSPVKDSAESYR 98
[Close]
BLAST of hesl-1 protein vs. Planmine SMEST
Match: SMESG000053680.1 (SMESG000053680.1)

HSP 1 Score: 347.821 bits (891), Expect = 2.210e-123
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 3 
Query:  171 MDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE 683
            MDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIV+RKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSM EFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE
Sbjct:    1 MDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQIVDRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMPEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE 171
[Close]
BLAST of hesl-1 protein vs. Planmine SMEST
Match: SMESG000068772.1 (SMESG000068772.1)

HSP 1 Score: 54.299 bits (129), Expect = 6.962e-9
Identity = 54/188 (28.72%), Postives = 94/188 (50.00%), Query Frame = 3 
Query:  165 IKMDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGMKMEKAEILDRVVEFCDQ-IVNRKIYNQKKHFKIIPSNIGHINTVKSETNILLTNQTKPTNSMAE----FTDRTK------FVQRMPLSTLNPNQMNIIEF----PQTVEKRVFNPKFTADLNRYWSSQKKIWKPYE 683
            IK  +  K+ ++L+K +K  MERRRR R+N +LD+LK+++++     +S  +K+EKA+IL+R V+F       +K  N+     +   N G++   + E    L N   P+N        F +  K      F+QR+ +  +  N+ NI       P ++     + KF   LNR   +   +W+P+E
Sbjct:   12 IKKQSQSKQFSTLKKSNKPLMERRRRQRINFALDQLKTLLEENSVTKES-FVKLEKADILERAVKFMTHTTAEKKNLNKNPRHLLDSFNTGYV-ACEKEIMRFLRNLYGPSNPFLALVFNFLETNKDSINYSFIQRI-VPNVKVNEKNINNLKPGEPLSIGSSQLDLKF---LNR--RTYNSVWRPWE 191
The following BLAST results are available for this feature:
BLAST of hesl-1 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hesl-1 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hesl-1 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
E(spl)m7-HLH9.957e-637.50gene:FBgn0002633 transcript:FBtr0084960[more]
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BLAST of hesl-1 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
hes2.11.148e-731.50hes family bHLH transcription factor 2, tandem dup... [more]
hes2.11.148e-731.50hes family bHLH transcription factor 2, tandem dup... [more]
hes2.21.852e-641.67hes family bHLH transcription factor 2, tandem dup... [more]
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BLAST of hesl-1 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
drc31.442e-744.26dynein regulatory complex subunit 3 [Source:Xenbas... [more]
HES22.308e-640.85hes family bHLH transcription factor 2 [Source:NCB... [more]
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BLAST of hesl-1 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Hes36.750e-833.70hes family bHLH transcription factor 3 [Source:MGI... [more]
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BLAST of hesl-1 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q00P32|HES2_XENLA1.176e-633.05Transcription factor HES-2 OS=Xenopus laevis OX=83... [more]
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BLAST of hesl-1 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U5YTN21.851e-10798.70Hesl-1 protein (Fragment) OS=Schmidtea mediterrane... [more]
A0A444UX165.820e-735.92Transcription factor HES-2 OS=Acipenser ruthenus O... [more]
A0A556U2251.790e-639.76Transcription factor HES-2 OS=Bagarius yarrelli OX... [more]
A0A3B3SU428.639e-640.26Hes family bHLH transcription factor 2 OS=Paramorm... [more]
A0A401RZF18.939e-630.43BHLH domain-containing protein OS=Chiloscyllium pu... [more]
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BLAST of hesl-1 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000035814.11.189e-640.68transcription factor HES-4-B-like [Source:NCBI gen... [more]
hes2.13.568e-638.10hes family bHLH transcription factor 2, tandem dup... [more]
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BLAST of hesl-1 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
her98.824e-643.86hairy-related 9 [Source:ZFIN;Acc:ZDB-GENE-011213-1... [more]
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BLAST of hesl-1 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hesl-1 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO450312.647e-634.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of hesl-1 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
HES21.166e-635.06hes family bHLH transcription factor 2 [Source:NCB... [more]
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BLAST of hesl-1 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000053680.12.210e-12398.83SMESG000053680.1[more]
SMESG000068772.16.962e-928.72SMESG000068772.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30006440
GenBankAGZ94925.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006440 ID=SMED30006440|Name=hesl-1 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=728bp
ATTGATTCGACACATTTCGCAATTTTTAATGCACTTGGTACCTCAGTAAC
AAATATCACAAAGAACTGCGAAAAAATAACATTTTGAAATTCGTCAAAAA
ATAAGAGCATATTATTGGACGCTGCTAAAGTACTTATCTTGAAGGGTACA
CTGAAACGGACATTATCAAAATGGACACGAGCCAAAAGAAAGAAAACAGC
TTAAGAAAGATTTCAAAACAAGAAATGGAAAGACGACGAAGGGCGCGAAT
GAACGTCAGTCTGGATAAATTAAAATCCATCATGCAGAAAGTCGGTTTTA
TAGATGATTCAGATGGAATGAAAATGGAAAAAGCGGAAATTCTGGACCGA
GTAGTTGAATTTTGTGATCAAATCGTTAACAGAAAGATTTATAATCAAAA
AAAACATTTCAAAATCATCCCGTCCAATATCGGTCATATTAATACAGTAA
AATCCGAAACAAATATTTTATTAACAAATCAAACCAAACCAACGAATTCC
ATGGCAGAATTTACAGACAGAACCAAATTCGTTCAACGTATGCCGTTGTC
AACATTAAATCCCAACCAAATGAACATTATTGAATTTCCACAAACAGTAG
AAAAAAGAGTATTCAATCCGAAATTCACAGCGGATTTAAACCGATATTGG
AGCTCACAGAAAAAGATTTGGAAGCCTTATGAATGATGTAAATTCTTGAT
TTGTAGTTTTGATTTAATAGATCGGAAG
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protein sequence of SMED30006440-orf-1

>SMED30006440-orf-1 ID=SMED30006440-orf-1|Name=SMED30006440-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=172bp
MDTSQKKENSLRKISKQEMERRRRARMNVSLDKLKSIMQKVGFIDDSDGM
KMEKAEILDRVVEFCDQIVNRKIYNQKKHFKIIPSNIGHINTVKSETNIL
LTNQTKPTNSMAEFTDRTKFVQRMPLSTLNPNQMNIIEFPQTVEKRVFNP
KFTADLNRYWSSQKKIWKPYE*
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Aliases
The feature 'hesl-1 protein' has the following synonyms
Synonym
hesl-1
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002109X1 cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 17..74
e-value: 2.0E-5
score: 34.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 12..65
e-value: 1.0E-11
score: 44.6
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 11..68
score: 11.782
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 3..70
e-value: 6.1E-14
score: 53.7
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 5..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR10985:SF77PROTEIN DEADPAN-RELATEDcoord: 3..97
NoneNo IPR availablePANTHERPTHR10985BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATEDcoord: 3..97