SMED30006391

Overview
NameSMED30006391
Smed IDSMED30006391
Length (bp)425
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006391 (SMED30006391) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006391 (SMED30006391) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006391 (SMED30006391) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006391 (SMED30006391) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30006391

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006391SMESG000026399.1 SmedASXL_061551SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30006391SMESG000026399.1 dd_Smed_v4_110_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30006391SMESG000026399.1 dd_Smed_v6_110_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30006391 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000821:91560..92115 -1999
Homology
BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Celegans
Match: rpl-34 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:A3QMC5])

HSP 1 Score: 103.219 bits (256), Expect = 2.247e-29
Identity = 52/105 (49.52%), Postives = 71/105 (67.62%), Query Frame = -1
Query:   27 RLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 341
            R+T RRRLSYNT SN++   +TPGG LV  Y KK+G +PKC D  ++LHGI   RP     + + ++TV+RAYGG    + VK+RI R+FLVEE K++ K+ K  
Sbjct:    4 RVTYRRRLSYNTTSNKKRLVKTPGGRLVVQYIKKRGQIPKCRDTGVKLHGITPARPIALRLLKRNERTVTRAYGGCLSPNAVKERITRAFLVEEQKIVNKVIKHQ 108          
BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0302369)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1
Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107          
BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0084973)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1
Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107          
BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0084972)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1
Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107          
BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34b (gene:FBgn0037686 transcript:FBtr0339176)

HSP 1 Score: 139.043 bits (349), Expect = 1.442e-42
Identity = 68/106 (64.15%), Postives = 81/106 (76.42%), Query Frame = -1
Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +L GI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLKGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107          
BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34b (gene:FBgn0037686 transcript:FBtr0082049)

HSP 1 Score: 139.043 bits (349), Expect = 1.442e-42
Identity = 68/106 (64.15%), Postives = 81/106 (76.42%), Query Frame = -1
Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +L GI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLKGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107          
BLAST of SMED30006391 vs. Ensembl Zebrafish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 112.464 bits (280), Expect = 1.038e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Zebrafish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 112.464 bits (280), Expect = 1.038e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Zebrafish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 112.464 bits (280), Expect = 1.038e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Xenopus
Match: kbtbd13 (Xenopus tropicalis ribosomal protein L34 (rpl34), mRNA. [Source:RefSeq mRNA;Acc:NM_001365119])

HSP 1 Score: 112.849 bits (281), Expect = 1.431e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Xenopus
Match: ENSXETT00000041593.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:5:26662937:26663375:1 gene:ENSXETG00000002546.1 transcript:ENSXETT00000041593.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.071 bits (266), Expect = 2.564e-30
Identity = 57/107 (53.27%), Postives = 73/107 (68.22%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYN  SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYG + C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNAASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPKVLMRLSKTKKHVSRAYGETMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 114.005 bits (284), Expect = 3.126e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 114.005 bits (284), Expect = 3.126e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 114.005 bits (284), Expect = 3.126e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 92.4337 bits (228), Expect = 4.246e-25
Identity = 51/89 (57.30%), Postives = 61/89 (68.54%), Query Frame = -1
Query:   87 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKR 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ R
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDR 90          
BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 61.2326 bits (147), Expect = 3.267e-13
Identity = 32/58 (55.17%), Postives = 40/58 (68.97%), Query Frame = -1
Query:  180 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAM 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAV 59          
BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P45842|RL34_AEDAL (60S ribosomal protein L34 OS=Aedes albopictus OX=7160 GN=RpL34 PE=2 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 2.111e-40
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = -1
Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY+Y KK+ TVPKCG CK +L GIK  RP ER R+ +R KTV+R +GG  CH C+++RIIR+FL++E K++  LK Q 
Sbjct:    2 VQRLTLRRRLSYNTKSNKRRVVRTPGGRLVYLYVKKQRTVPKCGQCKEKLSGIKPSRPSERPRMCRRLKTVTRTFGGVLCHRCLRERIIRAFLIDEQKVVKVLKAQQ 108          
BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|Q9NB34|RL34_OCHTR (60S ribosomal protein L34 OS=Ochlerotatus triseriatus OX=7162 GN=RpL34 PE=2 SV=3)

HSP 1 Score: 134.42 bits (337), Expect = 4.333e-40
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = -1
Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY+Y KK+ TVPKCG CK +L GIK  RP ER R+ +R KTV+R +GG  CH C+++RIIR+FL++E K++  LK Q 
Sbjct:    2 VQRLTLRRRLSYNTKSNKRRVVRTPGGRLVYLYVKKQRTVPKCGQCKEKLSGIKPSRPSERPRMCRRLKTVTRTFGGVLCHRCLRERIIRAFLIDEQKVVKVLKAQQ 108          
BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P87262|RL34A_YEAST (60S ribosomal protein L34-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL34A PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 8.912e-33
Identity = 59/105 (56.19%), Postives = 74/105 (70.48%), Query Frame = -1
Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RIIR+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIVKKVVKE 107          
BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P40525|RL34B_YEAST (60S ribosomal protein L34-B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL34B PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.537e-32
Identity = 58/105 (55.24%), Postives = 74/105 (70.48%), Query Frame = -1
Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RI+R+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIVRAFLIEEQKIVKKVVKE 107          
BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|Q9D1R9|RL34_MOUSE (60S ribosomal protein L34 OS=Mus musculus OX=10090 GN=Rpl34 PE=1 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 2.187e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3X0C8 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS6561 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.999e-47
Identity = 76/111 (68.47%), Postives = 92/111 (82.88%), Query Frame = -1
Query:   15 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN*FDK 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK GT PKCGDCK +L GIK  RP  RSR+SKR KTV+R+YGGSRCH+CV+ RI+R+FL+EE K++ ++ K+   D+
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKLGTFPKCGDCKRKLAGIKPSRPMTRSRMSKRLKTVTRSYGGSRCHACVRSRILRAFLMEEQKVLKQILKEKRMDR 111          
BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3TSS1 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS10710 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 5.177e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1
Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103          
BLAST of SMED30006391 vs. TrEMBL
Match: A0A564YRU5 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS8811 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.008e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1
Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103          
BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3SUY4 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS9342 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.008e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1
Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103          
BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3UL41 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8373 PE=4 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.316e-46
Identity = 74/103 (71.84%), Postives = 88/103 (85.44%), Query Frame = -1
Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSN+R K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP  R+R+SKR KTVSR YGGSRCH+CV+ RI+RSFL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNKRQKVKTPGGKLVYIYQKKRGTFPKCGDCKRKLAGIKPSRPMTRARMSKRLKTVSRTYGGSRCHACVRSRILRSFLMEEQKVLKQI 103          
BLAST of SMED30006391 vs. Ensembl Cavefish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:103026343])

HSP 1 Score: 115.546 bits (288), Expect = 6.076e-34
Identity = 62/107 (57.94%), Postives = 76/107 (71.03%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            MQRLT RRRLSYNT SNR+  +RTPG  +VY+Y KK G VPK  CG C   L G+ A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 MQRLTYRRRLSYNTASNRKRLSRTPGNRVVYLYTKKNGKVPKSACGICPGRLRGMCAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Sea Lamprey
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 117.472 bits (293), Expect = 2.771e-35
Identity = 61/109 (55.96%), Postives = 76/109 (69.72%), Query Frame = -1
Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLT RRRLSYNT SN+   ++TPGG LVY+Y KK G  PK  CG C   L G+KA+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K  
Sbjct:   13 VQRLTYRRRLSYNTASNKTRLSKTPGGRLVYLYMKKVGKAPKSACGLCPGRLRGVKAVRPAVLKRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQ 121          
BLAST of SMED30006391 vs. Ensembl Yeast
Match: RPL34A (Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication [Source:SGD;Acc:S000002135])

HSP 1 Score: 115.161 bits (287), Expect = 1.283e-34
Identity = 59/105 (56.19%), Postives = 74/105 (70.48%), Query Frame = -1
Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RIIR+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIVKKVVKE 107          
BLAST of SMED30006391 vs. Ensembl Yeast
Match: RPL34B (Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication [Source:SGD;Acc:S000001314])

HSP 1 Score: 114.39 bits (285), Expect = 2.214e-34
Identity = 58/105 (55.24%), Postives = 74/105 (70.48%), Query Frame = -1
Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RI+R+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIVRAFLIEEQKIVKKVVKE 107          
BLAST of SMED30006391 vs. Ensembl Nematostella
Match: EDO38420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCX9])

HSP 1 Score: 145.976 bits (367), Expect = 1.885e-46
Identity = 69/104 (66.35%), Postives = 85/104 (81.73%), Query Frame = -1
Query:   33 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 344
            QRLT RRRLSYNT SNRR  ++TPGG LVY+Y KK+G VPKCGDCK +L G+ AMRP +   +SK QKTVSRAYGGSRC +CVK+RIIR+FL+EE K++ ++ K
Sbjct:    3 QRLTFRRRLSYNTKSNRRRVSKTPGGRLVYLYTKKQGKVPKCGDCKKKLQGLPAMRPMKLMHISKPQKTVSRAYGGSRCAACVKERIIRAFLIEEQKIVVRVLK 106          
BLAST of SMED30006391 vs. Ensembl Medaka
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:101167741])

HSP 1 Score: 112.849 bits (281), Expect = 6.801e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1
Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108          
BLAST of SMED30006391 vs. Ensembl Medaka
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:101167741])

HSP 1 Score: 88.5817 bits (218), Expect = 3.306e-23
Identity = 53/117 (45.30%), Postives = 70/117 (59.83%), Query Frame = -1
Query:   27 LISKMQRLTLRRRLSYNTNSN----RRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 359
            ++SK   LT     SY+ N +        +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K  
Sbjct:   12 ILSKETHLT-----SYDHNVDPPPPHACSSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQ 123          
BLAST of SMED30006391 vs. Planmine SMEST
Match: SMESG000026399.1 (SMESG000026399.1)

HSP 1 Score: 217.238 bits (552), Expect = 1.881e-74
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = -1
Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN
Sbjct:    1 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 107          
The following BLAST results are available for this feature:
BLAST of SMED30006391 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL342.939e-3255.14ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL342.939e-3255.14ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL342.939e-3255.14ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL342.939e-3255.14ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC... [more]
RPL342.939e-3255.14ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of SMED30006391 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl-342.247e-2949.52Ribosomal Protein, Large subunit [Source:UniProtK... [more]
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BLAST of SMED30006391 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RpL34a5.447e-4465.09gene:FBgn0039406 transcript:FBtr0302369[more]
RpL34a5.447e-4465.09gene:FBgn0039406 transcript:FBtr0084973[more]
RpL34a5.447e-4465.09gene:FBgn0039406 transcript:FBtr0084972[more]
RpL34b1.442e-4264.15gene:FBgn0037686 transcript:FBtr0339176[more]
RpL34b1.442e-4264.15gene:FBgn0037686 transcript:FBtr0082049[more]
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BLAST of SMED30006391 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpl341.038e-3256.07ribosomal protein L34 [Source:NCBI gene;Acc:394097... [more]
rpl341.038e-3256.07ribosomal protein L34 [Source:NCBI gene;Acc:394097... [more]
rpl341.038e-3256.07ribosomal protein L34 [Source:NCBI gene;Acc:394097... [more]
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BLAST of SMED30006391 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
kbtbd131.431e-3256.07Xenopus tropicalis ribosomal protein L34 (rpl34), ... [more]
ENSXETT00000041593.12.564e-3053.27pep primary_assembly:Xenopus_tropicalis_v9.1:5:266... [more]
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BLAST of SMED30006391 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rpl343.126e-3356.07ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl343.126e-3356.07ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl343.126e-3356.07ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl344.246e-2557.30ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1... [more]
Rpl343.267e-1355.17ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of SMED30006391 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P45842|RL34_AEDAL2.111e-4060.7560S ribosomal protein L34 OS=Aedes albopictus OX=7... [more]
sp|Q9NB34|RL34_OCHTR4.333e-4060.7560S ribosomal protein L34 OS=Ochlerotatus triseria... [more]
sp|P87262|RL34A_YEAST8.912e-3356.1960S ribosomal protein L34-A OS=Saccharomyces cerev... [more]
sp|P40525|RL34B_YEAST1.537e-3255.2460S ribosomal protein L34-B OS=Saccharomyces cerev... [more]
sp|Q9D1R9|RL34_MOUSE2.187e-3256.0760S ribosomal protein L34 OS=Mus musculus OX=10090... [more]
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BLAST of SMED30006391 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3X0C82.999e-4768.47Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
A0A0R3TSS15.177e-4771.84Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A564YRU56.008e-4771.84Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0R3SUY46.008e-4771.84Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0R3UL411.316e-4671.84Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of SMED30006391 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl346.076e-3457.94ribosomal protein L34 [Source:NCBI gene;Acc:103026... [more]
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BLAST of SMED30006391 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl342.771e-3555.96ribosomal protein L34 [Source:NCBI gene;Acc:394097... [more]
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BLAST of SMED30006391 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPL34A1.283e-3456.19Ribosomal 60S subunit protein L34A; homologous to ... [more]
RPL34B2.214e-3455.24Ribosomal 60S subunit protein L34B; homologous to ... [more]
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BLAST of SMED30006391 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO384201.885e-4666.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30006391 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl346.801e-3356.07ribosomal protein L34 [Source:NCBI gene;Acc:101167... [more]
rpl343.306e-2345.30ribosomal protein L34 [Source:NCBI gene;Acc:101167... [more]
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BLAST of SMED30006391 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000026399.11.881e-74100.00SMESG000026399.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006391 ID=SMED30006391|Name=SMED30006391|organism=Schmidtea mediterranea sexual|type=transcript|length=425bp
AAATCCAAAATGTATTTATCAAATTAGTTTTGCTTCTTCAACTTAGCAAT
TAATTTATCCTCTTCAACTAAAAAACTTCTGATAATACGTTTCTTTACAC
ATGAATGGCATCTTGAACCTCCATAAGCTCTTGAAACAGTTTTTTGTCTT
TTACTGACGCGGCTTCGTTCACATGGTCTCATGGCTTTAATACCATGTAA
TTCCATTTTGCAGTCACCGCATTTTGGAACGGTACCCTTCTTCTTTTGAT
AAATATATACTAAATTTCCTCCGGGAGTACGGGTCTTAACACGACGGTTA
GAATTTGTGTTGTATGATAATCTTCTACGAAGAGTTAATCTTTGCATTTT
TGAAATTAGTTTTTAATAGTTTAATATCTTATTTAAACAATAATTTTTTA
CTTTTAACTTAAATTTTAACCGTCG
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protein sequence of SMED30006391-orf-1

>SMED30006391-orf-1 ID=SMED30006391-orf-1|Name=SMED30006391-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMEL
HGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLI
AKLKKQN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR038562Ribosomal_L34Ae_sf
IPR018065Ribosomal_L34e_CS
IPR019774Aromatic-AA_hydroxylase_C
IPR008195Ribosomal_L34Ae
Vocabulary: molecular function
TermDefinition
GO:0016714oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008195Ribosomal protein L34AePRINTSPR01250RIBOSOMALL34coord: 63..85
score: 46.58
coord: 25..42
score: 49.21
coord: 43..53
score: 46.75
coord: 12..25
score: 48.98
IPR008195Ribosomal protein L34AePFAMPF01199Ribosomal_L34ecoord: 1..93
e-value: 2.1E-41
score: 140.1
IPR008195Ribosomal protein L34AePANTHERPTHR1075960S RIBOSOMAL PROTEIN L34coord: 2..106
IPR038562Ribosomal protein L34Ae superfamilyGENE3DG3DSA:3.40.1800.40coord: 38..107
e-value: 1.3E-31
score: 110.2
NoneNo IPR availablePANTHERPTHR10759:SF0FI02850P-RELATEDcoord: 2..106
IPR018065Ribosomal protein L34e, conserved sitePROSITEPS01145RIBOSOMAL_L34Ecoord: 14..27