SMED30006391

Overview


Name	SMED30006391
Smed ID	SMED30006391
Length (bp)	425

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30006391 (SMED30006391) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006391 (SMED30006391) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006391 (SMED30006391) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006391 (SMED30006391) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30006391

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
X1 cell	SMED30006391	SmedASXL_061551	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
pharynx	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30006391	dd_Smed_v4_110_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30006391	dd_Smed_v6_110_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30006391 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000821:91560..92115 -	1999

Homology

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BLAST of SMED30006391 vs. Ensembl Human
Match: RPL34 (ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC:10340])

HSP 1 Score: 112.079 bits (279), Expect = 2.939e-32
Identity = 59/107 (55.14%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Celegans
Match: rpl-34 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:A3QMC5])

HSP 1 Score: 103.219 bits (256), Expect = 2.247e-29
Identity = 52/105 (49.52%), Postives = 71/105 (67.62%), Query Frame = -1

Query:   27 RLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 341
            R+T RRRLSYNT SN++   +TPGG LV  Y KK+G +PKC D  ++LHGI   RP     + + ++TV+RAYGG    + VK+RI R+FLVEE K++ K+ K  
Sbjct:    4 RVTYRRRLSYNTTSNKKRLVKTPGGRLVVQYIKKRGQIPKCRDTGVKLHGITPARPIALRLLKRNERTVTRAYGGCLSPNAVKERITRAFLVEEQKIVNKVIKHQ 108

[Close]

BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0302369)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1

Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107

[Close]

BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0084973)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1

Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107

[Close]

BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34a (gene:FBgn0039406 transcript:FBtr0084972)

HSP 1 Score: 142.51 bits (358), Expect = 5.447e-44
Identity = 69/106 (65.09%), Postives = 82/106 (77.36%), Query Frame = -1

Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +LHGI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLHGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107

[Close]

BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34b (gene:FBgn0037686 transcript:FBtr0339176)

HSP 1 Score: 139.043 bits (349), Expect = 1.442e-42
Identity = 68/106 (64.15%), Postives = 81/106 (76.42%), Query Frame = -1

Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +L GI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLKGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107

[Close]

BLAST of SMED30006391 vs. Ensembl Fly
Match: RpL34b (gene:FBgn0037686 transcript:FBtr0082049)

HSP 1 Score: 139.043 bits (349), Expect = 1.442e-42
Identity = 68/106 (64.15%), Postives = 81/106 (76.42%), Query Frame = -1

Query:   30 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY Y KK  TVP+CG CK +L GI   RP ER R+SKR KTVSR YGG  CHSC+++RI+R+FL+EE K++  LK Q
Sbjct:    2 VQRLTLRRRLSYNTRSNKRRIVRTPGGRLVYQYVKKNPTVPRCGQCKEKLKGITPSRPSERPRMSKRLKTVSRTYGGVLCHSCLRERIVRAFLIEEQKIVKALKSQ 107

[Close]

BLAST of SMED30006391 vs. Ensembl Zebrafish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 112.464 bits (280), Expect = 1.038e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKTGKSPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Xenopus
Match: kbtbd13 (Xenopus tropicalis ribosomal protein L34 (rpl34), mRNA. [Source:RefSeq mRNA;Acc:NM_001365119])

HSP 1 Score: 112.849 bits (281), Expect = 1.431e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Xenopus
Match: ENSXETT00000041593.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:5:26662937:26663375:1 gene:ENSXETG00000002546.1 transcript:ENSXETT00000041593.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.071 bits (266), Expect = 2.564e-30
Identity = 57/107 (53.27%), Postives = 73/107 (68.22%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYN  SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYG + C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNAASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPKVLMRLSKTKKHVSRAYGETMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 114.005 bits (284), Expect = 3.126e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 92.4337 bits (228), Expect = 4.246e-25
Identity = 51/89 (57.30%), Postives = 61/89 (68.54%), Query Frame = -1

Query:   87 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKR 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ R
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDR 90

[Close]

BLAST of SMED30006391 vs. Ensembl Mouse
Match: Rpl34 (ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1915686])

HSP 1 Score: 61.2326 bits (147), Expect = 3.267e-13
Identity = 32/58 (55.17%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  180 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAM 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAV 59

[Close]

BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P45842|RL34_AEDAL (60S ribosomal protein L34 OS=Aedes albopictus OX=7160 GN=RpL34 PE=2 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 2.111e-40
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = -1

Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY+Y KK+ TVPKCG CK +L GIK  RP ER R+ +R KTV+R +GG  CH C+++RIIR+FL++E K++  LK Q 
Sbjct:    2 VQRLTLRRRLSYNTKSNKRRVVRTPGGRLVYLYVKKQRTVPKCGQCKEKLSGIKPSRPSERPRMCRRLKTVTRTFGGVLCHRCLRERIIRAFLIDEQKVVKVLKAQQ 108

[Close]

BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|Q9NB34|RL34_OCHTR (60S ribosomal protein L34 OS=Ochlerotatus triseriatus OX=7162 GN=RpL34 PE=2 SV=3)

HSP 1 Score: 134.42 bits (337), Expect = 4.333e-40
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = -1

Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLTLRRRLSYNT SN+R   RTPGG LVY+Y KK+ TVPKCG CK +L GIK  RP ER R+ +R KTV+R +GG  CH C+++RIIR+FL++E K++  LK Q 
Sbjct:    2 VQRLTLRRRLSYNTKSNKRRVVRTPGGRLVYLYVKKQRTVPKCGQCKEKLSGIKPSRPSERPRMCRRLKTVTRTFGGVLCHRCLRERIIRAFLIDEQKVVKVLKAQQ 108

[Close]

BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P87262|RL34A_YEAST (60S ribosomal protein L34-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL34A PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 8.912e-33
Identity = 59/105 (56.19%), Postives = 74/105 (70.48%), Query Frame = -1

Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RIIR+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIVKKVVKE 107

[Close]

BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|P40525|RL34B_YEAST (60S ribosomal protein L34-B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL34B PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.537e-32
Identity = 58/105 (55.24%), Postives = 74/105 (70.48%), Query Frame = -1

Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RI+R+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIVRAFLIEEQKIVKKVVKE 107

[Close]

BLAST of SMED30006391 vs. UniProt/SwissProt
Match: sp|Q9D1R9|RL34_MOUSE (60S ribosomal protein L34 OS=Mus musculus OX=10090 GN=Rpl34 PE=1 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 2.187e-32
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L G++A+RP    R+SK QK VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTQKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3X0C8 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS6561 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.999e-47
Identity = 76/111 (68.47%), Postives = 92/111 (82.88%), Query Frame = -1

Query:   15 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN*FDK 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK GT PKCGDCK +L GIK  RP  RSR+SKR KTV+R+YGGSRCH+CV+ RI+R+FL+EE K++ ++ K+   D+
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKLGTFPKCGDCKRKLAGIKPSRPMTRSRMSKRLKTVTRSYGGSRCHACVRSRILRAFLMEEQKVLKQILKEKRMDR 111

[Close]

BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3TSS1 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS10710 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 5.177e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1

Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103

[Close]

BLAST of SMED30006391 vs. TrEMBL
Match: A0A564YRU5 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS8811 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.008e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1

Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103

[Close]

BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3SUY4 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS9342 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.008e-47
Identity = 74/103 (71.84%), Postives = 89/103 (86.41%), Query Frame = -1

Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSNRR K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP +R+R+SKR KTVSR YGGSRCH+CV+ RI+R+FL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNRRQKVKTPGGKLVYIYQKKRGTFPKCGDCKCKLAGIKPSRPMDRTRMSKRLKTVSRTYGGSRCHACVRSRILRAFLMEEQKVLKQI 103

[Close]

BLAST of SMED30006391 vs. TrEMBL
Match: A0A0R3UL41 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8373 PE=4 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.316e-46
Identity = 74/103 (71.84%), Postives = 88/103 (85.44%), Query Frame = -1

Query:   39 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKL 347
            MQRLTLRRRLSYNTNSN+R K +TPGG LVYIYQKK+GT PKCGDCK +L GIK  RP  R+R+SKR KTVSR YGGSRCH+CV+ RI+RSFL+EE K++ ++
Sbjct:    1 MQRLTLRRRLSYNTNSNKRQKVKTPGGKLVYIYQKKRGTFPKCGDCKRKLAGIKPSRPMTRARMSKRLKTVSRTYGGSRCHACVRSRILRSFLMEEQKVLKQI 103

[Close]

BLAST of SMED30006391 vs. Ensembl Cavefish
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:103026343])

HSP 1 Score: 115.546 bits (288), Expect = 6.076e-34
Identity = 62/107 (57.94%), Postives = 76/107 (71.03%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            MQRLT RRRLSYNT SNR+  +RTPG  +VY+Y KK G VPK  CG C   L G+ A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 MQRLTYRRRLSYNTASNRKRLSRTPGNRVVYLYTKKNGKVPKSACGICPGRLRGMCAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Sea Lamprey
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:394097])

HSP 1 Score: 117.472 bits (293), Expect = 2.771e-35
Identity = 61/109 (55.96%), Postives = 76/109 (69.72%), Query Frame = -1

Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            +QRLT RRRLSYNT SN+   ++TPGG LVY+Y KK G  PK  CG C   L G+KA+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K  
Sbjct:   13 VQRLTYRRRLSYNTASNKTRLSKTPGGRLVYLYMKKVGKAPKSACGLCPGRLRGVKAVRPAVLKRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQ 121

[Close]

BLAST of SMED30006391 vs. Ensembl Yeast
Match: RPL34A (Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication [Source:SGD;Acc:S000002135])

HSP 1 Score: 115.161 bits (287), Expect = 1.283e-34
Identity = 59/105 (56.19%), Postives = 74/105 (70.48%), Query Frame = -1

Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RIIR+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIVKKVVKE 107

[Close]

BLAST of SMED30006391 vs. Ensembl Yeast
Match: RPL34B (Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication [Source:SGD;Acc:S000001314])

HSP 1 Score: 114.39 bits (285), Expect = 2.214e-34
Identity = 58/105 (55.24%), Postives = 74/105 (70.48%), Query Frame = -1

Query:   30 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQ 344
            QR+T RRR  YNT SN+    +TPGG L   + KK  T PKCGDC   L GI  +RP + + VSK  KTVSRAYGGSRC +CVK+RI+R+FL+EE K++ K+ K+
Sbjct:    3 QRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIVRAFLIEEQKIVKKVVKE 107

[Close]

BLAST of SMED30006391 vs. Ensembl Nematostella
Match: EDO38420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCX9])

HSP 1 Score: 145.976 bits (367), Expect = 1.885e-46
Identity = 69/104 (66.35%), Postives = 85/104 (81.73%), Query Frame = -1

Query:   33 QRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 344
            QRLT RRRLSYNT SNRR  ++TPGG LVY+Y KK+G VPKCGDCK +L G+ AMRP +   +SK QKTVSRAYGGSRC +CVK+RIIR+FL+EE K++ ++ K
Sbjct:    3 QRLTFRRRLSYNTKSNRRRVSKTPGGRLVYLYTKKQGKVPKCGDCKKKLQGLPAMRPMKLMHISKPQKTVSRAYGGSRCAACVKERIIRAFLIEEQKIVVRVLK 106

[Close]

BLAST of SMED30006391 vs. Ensembl Medaka
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:101167741])

HSP 1 Score: 112.849 bits (281), Expect = 6.801e-33
Identity = 60/107 (56.07%), Postives = 75/107 (70.09%), Query Frame = -1

Query:   33 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKK 347
            +QRLT RRRLSYNT SN+   +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K
Sbjct:    2 VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLK 108

[Close]

BLAST of SMED30006391 vs. Ensembl Medaka
Match: rpl34 (ribosomal protein L34 [Source:NCBI gene;Acc:101167741])

HSP 1 Score: 88.5817 bits (218), Expect = 3.306e-23
Identity = 53/117 (45.30%), Postives = 70/117 (59.83%), Query Frame = -1

Query:   27 LISKMQRLTLRRRLSYNTNSN----RRVKTRTPGGNLVYIYQKKKGTVPK--CGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 359
            ++SK   LT     SY+ N +        +RTPG  +VY+Y KK G  PK  CG C   L GI+A+RP    R+SK +K VSRAYGGS C  CV+ RI R+FL+EE K++ K+ K  
Sbjct:   12 ILSKETHLT-----SYDHNVDPPPPHACSSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGIRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQ 123

[Close]

BLAST of SMED30006391 vs. Planmine SMEST
Match: SMESG000026399.1 (SMESG000026399.1)

HSP 1 Score: 217.238 bits (552), Expect = 1.881e-74
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = -1

Query:   27 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 347
            MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN
Sbjct:    1 MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMELHGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLIAKLKKQN 107

The following BLAST results are available for this feature:

BLAST of SMED30006391 vs. Ensembl Human
BLAST of SMED30006391 vs. Ensembl Celegans
BLAST of SMED30006391 vs. Ensembl Fly
BLAST of SMED30006391 vs. Ensembl Zebrafish
BLAST of SMED30006391 vs. Ensembl Xenopus
BLAST of SMED30006391 vs. Ensembl Mouse
BLAST of SMED30006391 vs. UniProt/SwissProt
BLAST of SMED30006391 vs. TrEMBL
BLAST of SMED30006391 vs. Ensembl Cavefish
BLAST of SMED30006391 vs. Ensembl Sea Lamprey
BLAST of SMED30006391 vs. Ensembl Yeast
BLAST of SMED30006391 vs. Ensembl Nematostella
BLAST of SMED30006391 vs. Ensembl Medaka
BLAST of SMED30006391 vs. Planmine SMEST

BLAST of SMED30006391 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RPL34	2.939e-32	55.14	ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC...	[more]
RPL34	2.939e-32	55.14	ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC...	[more]
RPL34	2.939e-32	55.14	ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC...	[more]
RPL34	2.939e-32	55.14	ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC...	[more]
RPL34	2.939e-32	55.14	ribosomal protein L34 [Source:HGNC Symbol;Acc:HGNC...	[more]

BLAST of SMED30006391 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rpl-34	2.247e-29	49.52	Ribosomal Protein, Large subunit [Source:UniProtK...	[more]

BLAST of SMED30006391 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RpL34a	5.447e-44	65.09	gene:FBgn0039406 transcript:FBtr0302369	[more]
RpL34a	5.447e-44	65.09	gene:FBgn0039406 transcript:FBtr0084973	[more]
RpL34a	5.447e-44	65.09	gene:FBgn0039406 transcript:FBtr0084972	[more]
RpL34b	1.442e-42	64.15	gene:FBgn0037686 transcript:FBtr0339176	[more]
RpL34b	1.442e-42	64.15	gene:FBgn0037686 transcript:FBtr0082049	[more]

BLAST of SMED30006391 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
rpl34	1.038e-32	56.07	ribosomal protein L34 [Source:NCBI gene;Acc:394097...	[more]
rpl34	1.038e-32	56.07	ribosomal protein L34 [Source:NCBI gene;Acc:394097...	[more]
rpl34	1.038e-32	56.07	ribosomal protein L34 [Source:NCBI gene;Acc:394097...	[more]

BLAST of SMED30006391 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
kbtbd13	1.431e-32	56.07	Xenopus tropicalis ribosomal protein L34 (rpl34), ...	[more]
ENSXETT00000041593.1	2.564e-30	53.27	pep primary_assembly:Xenopus_tropicalis_v9.1:5:266...	[more]

BLAST of SMED30006391 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rpl34	3.126e-33	56.07	ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1...	[more]
Rpl34	3.126e-33	56.07	ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1...	[more]
Rpl34	3.126e-33	56.07	ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1...	[more]
Rpl34	4.246e-25	57.30	ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1...	[more]
Rpl34	3.267e-13	55.17	ribosomal protein L34 [Source:MGI Symbol;Acc:MGI:1...	[more]

BLAST of SMED30006391 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P45842\|RL34_AEDAL	2.111e-40	60.75	60S ribosomal protein L34 OS=Aedes albopictus OX=7...	[more]
sp\|Q9NB34\|RL34_OCHTR	4.333e-40	60.75	60S ribosomal protein L34 OS=Ochlerotatus triseria...	[more]
sp\|P87262\|RL34A_YEAST	8.912e-33	56.19	60S ribosomal protein L34-A OS=Saccharomyces cerev...	[more]
sp\|P40525\|RL34B_YEAST	1.537e-32	55.24	60S ribosomal protein L34-B OS=Saccharomyces cerev...	[more]
sp\|Q9D1R9\|RL34_MOUSE	2.187e-32	56.07	60S ribosomal protein L34 OS=Mus musculus OX=10090...	[more]

BLAST of SMED30006391 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0R3X0C8	2.999e-47	68.47	Uncharacterized protein OS=Hydatigena taeniaeformi...	[more]
A0A0R3TSS1	5.177e-47	71.84	Uncharacterized protein OS=Rodentolepis nana OX=10...	[more]
A0A564YRU5	6.008e-47	71.84	Uncharacterized protein OS=Hymenolepis diminuta OX...	[more]
A0A0R3SUY4	6.008e-47	71.84	Uncharacterized protein OS=Hymenolepis diminuta OX...	[more]
A0A0R3UL41	1.316e-46	71.84	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]

BLAST of SMED30006391 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rpl34	6.076e-34	57.94	ribosomal protein L34 [Source:NCBI gene;Acc:103026...	[more]

BLAST of SMED30006391 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rpl34	2.771e-35	55.96	ribosomal protein L34 [Source:NCBI gene;Acc:394097...	[more]

BLAST of SMED30006391 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
RPL34A	1.283e-34	56.19	Ribosomal 60S subunit protein L34A; homologous to ...	[more]
RPL34B	2.214e-34	55.24	Ribosomal 60S subunit protein L34B; homologous to ...	[more]

BLAST of SMED30006391 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO38420	1.885e-46	66.35	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30006391 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rpl34	6.801e-33	56.07	ribosomal protein L34 [Source:NCBI gene;Acc:101167...	[more]
rpl34	3.306e-23	45.30	ribosomal protein L34 [Source:NCBI gene;Acc:101167...	[more]

BLAST of SMED30006391 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000026399.1	1.881e-74	100.00	SMESG000026399.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30006391 ID=SMED30006391|Name=SMED30006391|organism=Schmidtea mediterranea sexual|type=transcript|length=425bp
AAATCCAAAATGTATTTATCAAATTAGTTTTGCTTCTTCAACTTAGCAAT
TAATTTATCCTCTTCAACTAAAAAACTTCTGATAATACGTTTCTTTACAC
ATGAATGGCATCTTGAACCTCCATAAGCTCTTGAAACAGTTTTTTGTCTT
TTACTGACGCGGCTTCGTTCACATGGTCTCATGGCTTTAATACCATGTAA
TTCCATTTTGCAGTCACCGCATTTTGGAACGGTACCCTTCTTCTTTTGAT
AAATATATACTAAATTTCCTCCGGGAGTACGGGTCTTAACACGACGGTTA
GAATTTGTGTTGTATGATAATCTTCTACGAAGAGTTAATCTTTGCATTTT
TGAAATTAGTTTTTAATAGTTTAATATCTTATTTAAACAATAATTTTTTA
CTTTTAACTTAAATTTTAACCGTCG

protein sequence of SMED30006391-orf-1

>SMED30006391-orf-1 ID=SMED30006391-orf-1|Name=SMED30006391-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
MQRLTLRRRLSYNTNSNRRVKTRTPGGNLVYIYQKKKGTVPKCGDCKMEL
HGIKAMRPCERSRVSKRQKTVSRAYGGSRCHSCVKKRIIRSFLVEEDKLI
AKLKKQN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000429	neoblast
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR038562	Ribosomal_L34Ae_sf
IPR018065	Ribosomal_L34e_CS
IPR019774	Aromatic-AA_hydroxylase_C
IPR008195	Ribosomal_L34Ae

Vocabulary: **molecular function**
Term	Definition
GO:0016714	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
GO:0003735	structural constituent of ribosome

Vocabulary: **biological process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0006412	translation

Vocabulary: **cellular component**
Term	Definition
GO:0005840	ribosome
GO:0005622	intracellular

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008195	Ribosomal protein L34Ae	PRINTS	PR01250	RIBOSOMALL34	coord: 63..85 score: 46.58 coord: 25..42 score: 49.21 coord: 43..53 score: 46.75 coord: 12..25 score: 48.98
IPR008195	Ribosomal protein L34Ae	PFAM	PF01199	Ribosomal_L34e	coord: 1..93 e-value: 2.1E-41 score: 140.1
IPR008195	Ribosomal protein L34Ae	PANTHER	PTHR10759	60S RIBOSOMAL PROTEIN L34	coord: 2..106
IPR038562	Ribosomal protein L34Ae superfamily	GENE3D	G3DSA:3.40.1800.40		coord: 38..107 e-value: 1.3E-31 score: 110.2
None	No IPR available	PANTHER	PTHR10759:SF0	FI02850P-RELATED	coord: 2..106
IPR018065	Ribosomal protein L34e, conserved site	PROSITE	PS01145	RIBOSOMAL_L34E	coord: 14..27