SMED30006345
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006345 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30006345 aligns in the following genomic locations:
Homology
BLAST of SMED30006345 vs. Planmine SMEST
Match: SMESG000050291.1 (SMESG000050291.1) HSP 1 Score: 912.909 bits (2358), Expect = 0.000e+0 Identity = 459/464 (98.92%), Postives = 460/464 (99.14%), Query Frame = 2 Query: 32 MYIYIYSVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNXXXXXXXXXXPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSIIHNKVIAPFTISSLHPLKSHFKGLHFILFGALSLFVIILLITFTKFGIGPLLRQRFMNIQSKRVLSNPYITELVTTRSNELEILEN 1423 MYIYIY VYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNTTDKD FKFFPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKV+KKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKI NSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSIIHNKVIAPFTISSLHPLKSHFK LHFILFGALSLFVIILLITFTKFGIGPLLRQRFMNIQSKRVLSNPYITELVTTRSNELEILEN Sbjct: 1 MYIYIYLVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNTTDKDSFKFFPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVNKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKILNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSIIHNKVIAPFTISSLHPLKSHFKELHFILFGALSLFVIILLITFTKFGIGPLLRQRFMNIQSKRVLSNPYITELVTTRSNELEILEN 464
BLAST of SMED30006345 vs. Planmine SMEST
Match: SMESG000050295.1 (SMESG000050295.1) HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0 Identity = 368/417 (88.25%), Postives = 384/417 (92.09%), Query Frame = 2 Query: 38 IYIYSVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNXXXXXXXXXXPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSIIHNKVIAPFTISSLHPLKSHFKGLH--FILFGALSLFVIIL 1282 I ++ YLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVEN+IFVKIDV RPKPELIVA+NQKIP+KI+TMNEICGCSACFNIFMTFKFHLDLSLIDIEC NKSNIILLKNTTDKD FKFFPRPKSYKSSEKIL+IWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQH MKNYCLLEI+LKNGTILMLHIDRHNHLRMNIKH KTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNIS+ + SS +P + L FI+F L+ F+I+L Sbjct: 7 ILLFITYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENDIFVKIDVDRPKPELIVAYNQKIPSKISTMNEICGCSACFNIFMTFKFHLDLSLIDIECANKSNIILLKNTTDKDSFKFFPRPKSYKSSEKILFIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHIMKNYCLLEIILKNGTILMLHIDRHNHLRMNIKHSKTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISIR------LEMILFSSQNPSRYSILDLKEIFIIFLVLASFLILL 417
BLAST of SMED30006345 vs. Planmine SMEST
Match: SMESG000050295.1 (SMESG000050295.1) HSP 1 Score: 278.1 bits (710), Expect = 6.702e-89 Identity = 142/179 (79.33%), Postives = 151/179 (84.36%), Query Frame = 2 Query: 752 TFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSIIHNKVIAPFTISSLHPLKSHFKGLH--FILFGALSLFVIIL 1282 FSRHYEKEFDSRKNSERIKLMRIDWIRDQH MKNYCLLEI+LKNGTILMLHIDRHNHLRMNIKH KTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNIS+ + SS +P + L FI+F L+ F+I+L Sbjct: 135 AFSRHYEKEFDSRKNSERIKLMRIDWIRDQHIMKNYCLLEIILKNGTILMLHIDRHNHLRMNIKHSKTKLLINTNLNKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISIR------LEMILFSSQNPSRYSILDLKEIFIIFLVLASFLILL 307 HSP 2 Score: 236.113 bits (601), Expect = 9.144e-73 Identity = 123/147 (83.67%), Postives = 134/147 (91.16%), Query Frame = 2 Query: 38 IYIYSVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNXXXXXXXXXXPRP-KSYKSSEKI 475 I ++ YLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVEN+IFVKIDV RPKPELIVA+NQKIP+KI+TMNEICGCSACFNIFMTFKFHLDLSLIDIEC NKSNIILLKNTTDK F + + + S K+SE+I Sbjct: 7 ILLFITYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENDIFVKIDVDRPKPELIVAYNQKIPSKISTMNEICGCSACFNIFMTFKFHLDLSLIDIECANKSNIILLKNTTDKAFSRHYEKEFDSRKNSERI 153
BLAST of SMED30006345 vs. Planmine SMEST
Match: SMESG000050289.1 (SMESG000050289.1) HSP 1 Score: 177.948 bits (450), Expect = 1.096e-49 Identity = 134/484 (27.69%), Postives = 237/484 (48.97%), Query Frame = 2 Query: 35 YIYIYSVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDNKSNIILLKNXXXXXXXXXXPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRH--YEKEFDSRKNSERIKLMRIDW-IRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKISN--------SLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISVSI------IHNKVIAPFTISSLHPLKSHFKGLH-----FILFGALSLFVIILLITFTKFGIGPLLRQRFMNIQSKRVLSNPYITELVTTRSNELEILE 1420 Y SV L+ F + + YIY++ G + G+ F ++I N G++FY D +N + ++I+ + + N + ++ T +C CS C N+++ L I I C+ ++N++ TD D F +P P Y +++ Y WMGNIG + G YL L +Q+ GCLD + K +K ++ G F + C++LY EKVC +S C N+ YKMYDCS S+N K+C S + +K + ++ + + R+DW + +K + L+EI LK+ T+L+ +ID ++ M+ + + NT++ S +LL+ L S ++K++ + Y + E+V+I +G Q + F G I+ I N F S++ P + L I+FG + +I+L+I K ++++ + K + +N Y +E V+T S + +LE Sbjct: 7 YFLFLSVLLSKFNGMFSEYIYEKFDGNMVSDKGMNFSVFIKNFKGVLFYCVDSKNRLLIQINKKSTETIITFKFNSYVTSEDMTSKRLCKCSNCSNVYLQLSILLQQLEILINCNKQANVVKFNKETDSDEFSEYPTPPDYNNNQTKTYFWMGNIGPFTGSKNKIYLALTSQLSGCLDKFYFNGKNIKF-KKSGKIFQSFCEVLYKEKVCNESRCSNNETHYKMYDCSPLSMNGKHCETILSDYAIVQKSYIRKQRWKEVIYFRLDWQMEVNSKIKRFSLMEIKLKDETLLLFYIDSTRNIVMSTLN----ISANTDIVDFSMKSDLNHRFTLLIILGESETTIKLNDCSNILTYIKKNIFEFVLINIGTIQSDPSFSLFEGKINFQFNPKYWKISNGNSDFFLTSTIIPNRVQPSNLSKYYLCVIVFGGI---IIMLVIILRKCKFRQKMKRQSQKDKWKDIQNNSYESESVSTPSAKNLVLE 482
BLAST of SMED30006345 vs. Planmine SMEST
Match: SMESG000050315.1 (SMESG000050315.1) HSP 1 Score: 169.474 bits (428), Expect = 1.863e-46 Identity = 107/377 (28.38%), Postives = 194/377 (51.46%), Query Frame = 2 Query: 41 YIYSVYLNIFLWLN---ASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENNIFVKIDVHRPKPELIVAHNQKIPNKIATMNEICGCSACFNIFMTFKFHLDLSLIDIECDN-KSNIILLKNXXXXXXXXXXPRPKSYKSSEKILYIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNSERIKLMRIDWIRDQHFMKNYCLLEILLKNGTILMLHIDRHNHLRMNIKHPKTKLLINTNLNKI----SNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLG 1147 + Y + L +++N A+Y Y+++ G + F I++ SG++ Y+ D NN+ ++I+ K + + N I +A E+CGCS+C +I+ TF +++ I+C+ S + + K + F +P P YKS++ ++WMGNIG + G + M Y PLK Q+ GCLD ++ K ++ + G F +C YN+ +C ++C Q+ Y M DCS++SLN +YC+ R E S K + ++ L+R++W+ + + + CL+E+ LKNG I++ H+D+ +M I + + KL T + + +N L N + +K+ E + + E ++ GK +F G Sbjct: 3 FFYEIQLTSIIFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIE----KDTVFIKLNDTI--SLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKKANQFFGYPIPNGYKSNKT--FLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQF-KSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNL-RSLNTEHSSIKLNHKLSLIRLEWMTNDD-LADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCNDFSG 368 The following BLAST results are available for this feature:
BLAST of SMED30006345 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006345 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006345 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006345 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006345 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006345 ID=SMED30006345|Name=SMED30006345|organism=Schmidtea mediterranea sexual|type=transcript|length=1501bpback to top protein sequence of SMED30006345-orf-1 >SMED30006345-orf-1 ID=SMED30006345-orf-1|Name=SMED30006345-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=469bp MYIYIYSVYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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