SMED30006335

Overview
NameSMED30006335
Smed IDSMED30006335
Length (bp)3393
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006335 (SMED30006335) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006335 (SMED30006335) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006335 (SMED30006335) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006335 (SMED30006335) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006335

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30006335SMESG000046906.1 SmedASXL_017626SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30006335SMESG000046906.1 dd_Smed_v6_10831_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30006335SMESG000046906.1 dd_Smed_v4_10831_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30006335 vs. TrEMBL
Match: A0A210PT82 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT13316 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.502e-16
Identity = 70/306 (22.88%), Postives = 162/306 (52.94%), Query Frame = 2 
Query:  365 LQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRN-DSVVANLKSNLNNLLGVNKKQEELLAESAKKLIE-LEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDKQTAIIRVERDQAINQKSSVMAKLD 1276
            L+K L+D + ++  + ++ QE +   +++K +F S IE LK    +  TN +  + +L+  E  N ++++A ++  ++ L    + QEE L ++ +++ +       +  A    R +L++ E +RG  F         +P ++V  +++C +E   D+  +S+KI + E +++ LK N+ HDKE  + E ++++  +  ++    +  +E   NA ++   ++ +  M++ + + ++     +I  LE  ++ +  +   +   W  +K+++E+ L++  K+ + +R ERD AI   SS+  KL+
Sbjct:  184 LEKALKDSQEQVTDLRRRLQEANDQSELQKRQFRSNIEELKGKLHETVTNRE-AVLDLRVKESANQETLLAKMQGQVHQLTEKCRAQEEALGDAGRQMDDNTRDKYLSDTALNQTRQILVDAERKRGRPFFDSDPASKQAPGMLVHTLERCMQEMSADITQKSQKIHELEKEILSLKHNISHDKETLIREQQDKISSMTTEHEKQMQSASERANNARKQTVTLQSQLAMIEGQFEQQMKMKDVMILDLEQKVRHLKEEFSDDRTKWQQKKETLEESLEKTHKEVSAMRKERDDAIRNHSSLEGKLE 488
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BLAST of SMED30006335 vs. Planmine SMEST
Match: SMESG000046906.1 (SMESG000046906.1)

HSP 1 Score: 1976.06 bits (5118), Expect = 0.000e+0
Identity = 1072/1084 (98.89%), Postives = 1073/1084 (98.99%), Query Frame = 2 
Query:    2 MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDKQTAIIRVERDQAINQKSSVMAKLDAXXXXXXXXXXXXXXXXSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYXXXXXXXXXXXXNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVLIQTRVDVINETFFKLEERCRQLEMQSSXXXXXXXXXXXXXXXXIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQXXXXXXXXXXXXMEKKICNLNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLLESKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNC-TESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP 3250
            MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDK TAIIRVERDQAINQKSSVMAKLDATQNKLTETETQLLQLQSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYEIEEILHEIMLMNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVLIQTRVD        LEERCRQLEMQSSGGNQNLNQNNEVGQVNIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQHLKSSKSNKHLSMEKKIC+LNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLL SKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNC TESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP
Sbjct:    1 MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDKLTAIIRVERDQAINQKSSVMAKLDATQNKLTETETQLLQLQSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYEIEEILHEIMLMNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVLIQTRVD--------LEERCRQLEMQSSGGNQNLNQNNEVGQVNIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQHLKSSKSNKHLSMEKKICDLNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLLASKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNCSTESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP 1076
[Close]
BLAST of SMED30006335 vs. Planmine SMEST
Match: SMESG000046906.1 (SMESG000046906.1)

HSP 1 Score: 1963.35 bits (5085), Expect = 0.000e+0
Identity = 1066/1083 (98.43%), Postives = 1067/1083 (98.52%), Query Frame = 2 
Query:    2 MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDKQTAIIRVERDQAINQKSSVMAKLDAXXXXXXXXXXXXXXXXSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYXXXXXXXXXXXXNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVLIQTRVDVINETFFKLEERCRQLEMQSSXXXXXXXXXXXXXXXXIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQXXXXXXXXXXXXMEKKICNLNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLLESKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNCTESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP 3250
            MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDK TAIIRVERDQAINQKSSVMAKLDATQNKLTETETQLLQLQSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYEIEEILHEIMLMNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVL              LEERCRQLEMQSSGGNQNLNQNNEVGQVNIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQHLKSSKSNKHLSMEKKIC+LNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLL SKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNCTESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP
Sbjct:    1 MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLRSQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRSIPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKMEKEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNLLGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFIKDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLLHDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLKAKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDENDKLTAIIRVERDQAINQKSSVMAKLDATQNKLTETETQLLQLQSDKYGLDKQIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNNFLLDLEVSEEENVPWRPRKYEIEEILHEIMLMNKQKNETIEKYDKIRKEFEILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIERLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVL--------------LEERCRQLEMQSSGGNQNLNQNNEVGQVNIIDTSKGKNQILREERNRLVIQMNELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKRLIIKLKDADSEITSLCQELQHLKSSKSNKHLSMEKKICDLNSELLSYKNELKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNSLEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRLREKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNESIHQLRGELQESIKEAKKLLASKTLNSSKDIDLKSQDISSDSSRYPMFKDEIYTERKRSLSDREQNNRFHMNSNNNCTESENVDSSTLNTKRSESTSLTEVDRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSP 1069
The following BLAST results are available for this feature:
BLAST of SMED30006335 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A210PT822.502e-1622.88Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
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BLAST of SMED30006335 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006335 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000046906.10.000e+098.89SMESG000046906.1[more]
SMESG000046906.10.000e+098.43SMESG000046906.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006335 ID=SMED30006335|Name=SMED30006335|organism=Schmidtea mediterranea sexual|type=transcript|length=3393bp
AATGAATAAATACAAGACAAAATATAATAATGATGGCAGAAATCGAAGTG
ATTTTAATAAATATTCCGATCATAAATTCCAAATTTCAACAGAAAATTGT
GATATATCACAAAAAATAAAGAACTTGGAATTAGAAGGTTTGAAATTAAG
AAGCCAAGTTGAAAGTATTTTGCATGAAACAAATGAAGACAGCACTTCGA
GAACTTGCGAATCTATGAATGAATGTGAAAGTTTAAACACGATTACAGAT
CAAAATGCAATTTATGATTCAAATTTTTCATGTGATGATTACAATAGATC
TATTCCAAAGTCTTATGATTGCAAACAGAATAAAAACTATAATTCTCGAG
ACAATATAATGAGTTTACAGAAAGAATTAGAAGATTGTCGAGGGAAACTA
GATTTCATGCATCAGAAATTTCAAGAAGAAAGTTATGCTCATAAAATGGA
AAAGGAAGAATTTGTCTCAACAATCGAAAATTTGAAAAGAACAAATTTTA
AAATCAAAACAAACAATGATCGACAAATTCAAGAGCTTAAATCTCACGAG
AAAAGAAACGATTCCGTTGTTGCTAACTTGAAATCCAATCTCAATAATCT
CCTGGGAGTTAATAAGAAGCAGGAAGAGCTTCTAGCAGAAAGTGCCAAAA
AATTAATTGAGCTAGAAGGTTCTCCTACAACTATAAAGGCATTTGAAGAT
ATACGAGATTTGCTCATTGAAATTGAATATCAGAGAGGTGCAGATTTTAT
TAAGGATCAAGATATAGAATCATGCTCTCCGAATATTATTGTCGACATTG
TTGATAAGTGCTTTAAGGAGTTTATGAATGACTTGGATGATAGAAGTCGA
AAGATAAGGCAACAAGAAGACGATATGATCCAATTAAAAAGAAATCTCCT
ACACGACAAAGAAAAATCTTTACTAGAGATAAAGAATCAAGTGGTCTATT
TAGAAGAACAAAACACCTATCTAAAAAGGCATATCAATGAAATAACTGAA
AATGCTGACCGCGAATTGAGTAAAGTAAGAGAAGAGTCACTGATGCTGAA
AGCAAAGTTAGACAATGAAATTTCGAATGCCACCTCTGTGATTGATGGAC
TGGAAACGAATTTACAACAGATGTCAAATAAGCTCAAGAGAGAAAACGAA
AATTGGAATTTAGAAAAGAAGTCAATGGAAAAGCTTTTAGATGAAAACGA
TAAACAAACAGCAATTATCCGAGTTGAGCGGGATCAGGCAATAAATCAAA
AATCGTCAGTTATGGCCAAGCTAGACGCAACACAAAATAAATTAACAGAA
ACAGAAACTCAACTCTTGCAGCTCCAATCGGATAAATATGGTCTCGATAA
ACAAATCGAAGTTCTAAAGAAACGAAATAAAGCTATTGAGAGTAGTTTAT
CGTTTACAAGAATTTATTTAGAAAATATAACCAAAAGTATTCGATCTTCT
CATAGAGGAGACTGTCTCGATTCCAGTTTCATTGAAACGAACTCAAACAA
TTTCCTACTGGACTTAGAAGTTTCTGAAGAAGAGAATGTTCCTTGGAGAC
CACGAAAATATGAAATTGAAGAAATACTTCACGAGATTATGTTGATGAAT
AAACAGAAAAATGAGACAATAGAAAAATATGATAAAATAAGAAAAGAATT
TGAAATACTTCAGAAAGAATTAGATTGTGCAAAATTTGAAAATAAAAAAT
TTGACCAAAATTTAAAAGCCTTGCAACTTGAAATTGAAAGTTCAAGAAAT
GAAAATTCTCACCTCAACAGTTTAATAGCCAATAGAAATGGAGAAATCGA
ACGGTTGACAGCGGAACGTGATTACTATTTTCAAATGTTAAATGAAAAAT
CAGAAGATTTGGCAATTGTAAAAACAGATAAACACAGGCTTAATGTTTTG
ATTCAAACCAGAGTCGATGTAATTAATGAGACTTTCTTTAAATTAGAAGA
GCGTTGTCGGCAACTGGAAATGCAATCCAGTGGCGGCAATCAGAATTTGA
ATCAAAACAATGAAGTGGGCCAAGTGAATATTATTGACACTTCAAAGGGA
AAAAATCAAATACTTCGAGAAGAAAGAAACAGGCTCGTAATTCAAATGAA
TGAATTGCAAGATTCCAGAGAACAGTTATTAAAGAACCAAAAAGACCAAA
CTGTTTGTATTGAACATATTAAAAACGAAAACATGCACTTGCAATTGGAG
CTAAAGAATCTTTATCGAGATTATGACGAACTGATGATTGAAAATAAGCG
GCTGATTATTAAATTGAAAGACGCAGATTCTGAAATAACCAGTCTTTGCC
AAGAACTTCAACATTTGAAAAGTTCAAAGTCGAACAAGCATTTAAGTATG
GAAAAGAAAATATGCAATCTTAATTCCGAACTTTTAAGTTATAAGAATGA
ATTGAAAATTTCACAATCTCAAATAAAAAGTTTTGAGAGACTCTCTAAGA
AATCAAATCATAATTCCCAACCTCCGGTAATTTTAAATGATATTAACAAA
ACAAATTTGAATCATGATTCATTGCAAATTACAAAAAGTCGTTTAAATTC
ACTGGAAAAACAAATGAATTCTATGCAGCTTGGTCATGAGAGCATGAAGG
AAGAAAATCAAAAGCTAACGGAACTGTTAAGAAAAGTTACGCATCAAAAT
GATAGAATGGCAGAAGAACTTGATACATACATCGAAGAAAATGGACGATT
ACGGGAGAAGATAAATATGAAATCTAATGAAGAAAATATTAAAATTAGCA
AATATTCAGAATATCAAACGAAAAATAAATATCCCAACAAATCTATGGTT
AAAAAAAAAGCTTCCGACCTAACCAGTGAAATATTGAACAATAATGAATC
TATTCACCAGCTCCGAGGGGAACTCCAAGAGTCTATTAAAGAAGCAAAAA
AACTTTTAGAGTCAAAAACATTAAATAGTTCCAAAGATATTGACTTAAAG
TCTCAAGATATTTCCTCTGATTCTTCAAGATATCCAATGTTTAAAGACGA
GATTTATACGGAAAGGAAACGATCTCTCAGTGACCGTGAACAGAACAATA
GATTTCATATGAATTCAAATAATAATTGCACCGAATCCGAAAATGTCGAT
TCGTCTACATTAAACACAAAACGATCAGAATCGACTTCATTAACAGAAGT
GGATCGTCATTTGAATCTGCCTCCAGTAAAACAAAATGACAGTCAATATA
AAGCTCAAAATCAACGGTCTTCCAGTCCAATAGAAAATCTACTTTCTCCA
GGTTTTTTCATGTTTAAATATACAATTAACCATTTTTTTACAAATACTGG
TTTATGGGAGGGAAGTACCTTTAATACATATATTAATTTAATTTTTAAAT
TTCAAATGCATTTTTAAATTAAATTAAAATTTTGTTTTAATAT
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protein sequence of SMED30006335-orf-1

>SMED30006335-orf-1 ID=SMED30006335-orf-1|Name=SMED30006335-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1122bp
MNKYKTKYNNDGRNRSDFNKYSDHKFQISTENCDISQKIKNLELEGLKLR
SQVESILHETNEDSTSRTCESMNECESLNTITDQNAIYDSNFSCDDYNRS
IPKSYDCKQNKNYNSRDNIMSLQKELEDCRGKLDFMHQKFQEESYAHKME
KEEFVSTIENLKRTNFKIKTNNDRQIQELKSHEKRNDSVVANLKSNLNNL
LGVNKKQEELLAESAKKLIELEGSPTTIKAFEDIRDLLIEIEYQRGADFI
KDQDIESCSPNIIVDIVDKCFKEFMNDLDDRSRKIRQQEDDMIQLKRNLL
HDKEKSLLEIKNQVVYLEEQNTYLKRHINEITENADRELSKVREESLMLK
AKLDNEISNATSVIDGLETNLQQMSNKLKRENENWNLEKKSMEKLLDEND
KQTAIIRVERDQAINQKSSVMAKLDATQNKLTETETQLLQLQSDKYGLDK
QIEVLKKRNKAIESSLSFTRIYLENITKSIRSSHRGDCLDSSFIETNSNN
FLLDLEVSEEENVPWRPRKYEIEEILHEIMLMNKQKNETIEKYDKIRKEF
EILQKELDCAKFENKKFDQNLKALQLEIESSRNENSHLNSLIANRNGEIE
RLTAERDYYFQMLNEKSEDLAIVKTDKHRLNVLIQTRVDVINETFFKLEE
RCRQLEMQSSGGNQNLNQNNEVGQVNIIDTSKGKNQILREERNRLVIQMN
ELQDSREQLLKNQKDQTVCIEHIKNENMHLQLELKNLYRDYDELMIENKR
LIIKLKDADSEITSLCQELQHLKSSKSNKHLSMEKKICNLNSELLSYKNE
LKISQSQIKSFERLSKKSNHNSQPPVILNDINKTNLNHDSLQITKSRLNS
LEKQMNSMQLGHESMKEENQKLTELLRKVTHQNDRMAEELDTYIEENGRL
REKINMKSNEENIKISKYSEYQTKNKYPNKSMVKKKASDLTSEILNNNES
IHQLRGELQESIKEAKKLLESKTLNSSKDIDLKSQDISSDSSRYPMFKDE
IYTERKRSLSDREQNNRFHMNSNNNCTESENVDSSTLNTKRSESTSLTEV
DRHLNLPPVKQNDSQYKAQNQRSSSPIENLLSPGFFMFKYTINHFFTNTG
LWEGSTFNTYINLIFKFQMHF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: biological process
TermDefinition
GO:0000042protein targeting to Golgi
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 190..210
NoneNo IPR availableCOILSCoilCoilcoord: 278..305
NoneNo IPR availableCOILSCoilCoilcoord: 848..910
NoneNo IPR availableCOILSCoilCoilcoord: 720..775
NoneNo IPR availableCOILSCoilCoilcoord: 350..412
NoneNo IPR availableCOILSCoilCoilcoord: 685..712
NoneNo IPR availableCOILSCoilCoilcoord: 951..971
NoneNo IPR availableCOILSCoilCoilcoord: 424..444
NoneNo IPR availableCOILSCoilCoilcoord: 119..139
NoneNo IPR availableCOILSCoilCoilcoord: 536..591
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1005..1049
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1015..1044