DNA methyl-transferase

Overview
NameDNA methyl-transferase (AHB82105.1)
Smed IDSMED30006325
Length (bp)1444
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA methyl-transferase (SMED30006325) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA methyl-transferase (SMED30006325) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA methyl-transferase (SMED30006325) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA methyl-transferase (SMED30006325) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006325

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006325SMESG000017337.1 SmedASXL_013126SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
germ cellSMED30006325SMESG000017337.1 KF664583smed_ncbi_20200123PMID:24063805
Jaber-Hijazi et al., 2013		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
germline stem cellSMED30006325SMESG000017337.1 KF664583smed_ncbi_20200123PMID:24063805
Jaber-Hijazi et al., 2013		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Human
Match: TRDMT1 (tRNA aspartic acid methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2977])

HSP 1 Score: 233.417 bits (594), Expect = 1.459e-71
Identity = 146/398 (36.68%), Postives = 216/398 (54.27%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK-------GSLMQD---LEAISYKPY---FDRKLYQCNCPV---------CSGRSRSL-ENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHK-LIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGS-------------YYQMTSCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++V+E +SG+GG+  AL E  I   VVAA D+N +AN VY+YNF  +    K IE I+ E    L+ DM  MSPPCQP+TR G   D+ D RT +  H L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +       G ++ +   +E++  + Y    + K+ + N            CSG+   L + +      R  +    +S  +  D L +  + ++ L+  K L +    LDIV  +  ++ CF K Y   IEG+GS             Y  +T+  +  Q+ +LL+            LKLRYF+P+EIAN + FP  F FPE +T  QRYRLLGNS+NV VVA ++  L
Sbjct:    4 LRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLHILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLI------------LKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKIL 389
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Human
Match: TRDMT1 (tRNA aspartic acid methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2977])

HSP 1 Score: 121.709 bits (304), Expect = 2.256e-32
Identity = 67/152 (44.08%), Postives = 85/152 (55.92%), Query Frame = 2 
Query:   83 ALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK 535
            AL E  I   VVAA D+N +AN VY+YNF  +    K IE I+ E    L+ DM  MSPPCQP+TR                       Q  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +
Sbjct:    1 ALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTR----------------------LQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSE 130
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Human
Match: TRDMT1 (tRNA aspartic acid methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2977])

HSP 1 Score: 89.7373 bits (221), Expect = 7.750e-20
Identity = 52/146 (35.62%), Postives = 69/146 (47.26%), Query Frame = 2 
Query:  101 IQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK 535
            I   VVAA D+N +AN VY+YNF  +    K IE I+ E    L+ DM  MSPPCQP+TR+                                            +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +
Sbjct:    4 IPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRD--------------------------------------------LLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSE 105
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Human
Match: TRDMT1 (tRNA aspartic acid methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2977])

HSP 1 Score: 53.5286 bits (127), Expect = 7.145e-9
Identity = 23/44 (52.27%), Postives = 32/44 (72.73%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNF 169
            ++V+E +SG+GG+  AL E  I   VVAA D+N +AN VY+YNF
Sbjct:    4 LRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNF 47
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Human
Match: TRDMT1 (tRNA aspartic acid methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2977])

HSP 1 Score: 52.7582 bits (125), Expect = 1.543e-8
Identity = 23/44 (52.27%), Postives = 32/44 (72.73%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNF 169
            ++V+E +SG+GG+  AL E  I   VVAA D+N +AN VY+YNF
Sbjct:    4 LRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNF 47
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Fly
Match: Mt2 (gene:FBgn0028707 transcript:FBtr0110911)

HSP 1 Score: 227.254 bits (578), Expect = 5.355e-70
Identity = 134/372 (36.02%), Postives = 206/372 (55.38%), Query Frame = 2 
Query:   41 KVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCY-NKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRP-HYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISSLDYAK-------------RLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            +V+E FSGIGG+  A N   +   +VAA D+N +AN+VY +N+ S     + I+S+S + +  L A+M  MSPPCQP+TR G   D +D R+ A+ H   LI + +   YI  ENVKGFESS  +   +  L  S + ++EF+L+P QF VPN+R RYY IA+   KG+           P+   K+++   P    +++ L                +I+  +E +N++       L+ + VL K    +DI+  + +++ CF K Y    EG+GS Y   S DE+H++ +L+   D S+ D +K             +++LRYF+PRE+A  M FP++F FP   T  Q+YRLLGNS+NVKVV  ++  L
Sbjct:    4 RVLELFSGIGGMHYAFNYAQLDGQIVAALDVNTVANAVYAHNYGSNLVKTRNIQSLSVKEVTKLQANMLLMSPPCQPHTRQGLQRDTEDKRSDALTHLCGLIPECQELEYILMENVKGFESSQARNQFIESLERSGFHWREFILTPTQFNVPNTRYRYYCIAR---KGADF---------PFAGGKIWE-EMPGAIAQNQGL---------------SQIAEIVE-ENVSPDF----LVPDDVLTKRVLVMDIIHPAQSRSMCFTKGYTHYTEGTGSAYTPLSEDESHRIFELVKEIDTSNQDASKSEKILQQRLDLLHQVRLRYFTPREVARLMSFPENFEFPPETTNRQKYRLLGNSINVKVVGELIKLL 342
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Fly
Match: Mt2 (gene:FBgn0028707 transcript:FBtr0342603)

HSP 1 Score: 227.254 bits (578), Expect = 5.355e-70
Identity = 134/372 (36.02%), Postives = 206/372 (55.38%), Query Frame = 2 
Query:   41 KVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCY-NKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRP-HYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISSLDYAK-------------RLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            +V+E FSGIGG+  A N   +   +VAA D+N +AN+VY +N+ S     + I+S+S + +  L A+M  MSPPCQP+TR G   D +D R+ A+ H   LI + +   YI  ENVKGFESS  +   +  L  S + ++EF+L+P QF VPN+R RYY IA+   KG+           P+   K+++   P    +++ L                +I+  +E +N++       L+ + VL K    +DI+  + +++ CF K Y    EG+GS Y   S DE+H++ +L+   D S+ D +K             +++LRYF+PRE+A  M FP++F FP   T  Q+YRLLGNS+NVKVV  ++  L
Sbjct:    4 RVLELFSGIGGMHYAFNYAQLDGQIVAALDVNTVANAVYAHNYGSNLVKTRNIQSLSVKEVTKLQANMLLMSPPCQPHTRQGLQRDTEDKRSDALTHLCGLIPECQELEYILMENVKGFESSQARNQFIESLERSGFHWREFILTPTQFNVPNTRYRYYCIAR---KGADF---------PFAGGKIWE-EMPGAIAQNQGL---------------SQIAEIVE-ENVSPDF----LVPDDVLTKRVLVMDIIHPAQSRSMCFTKGYTHYTEGTGSAYTPLSEDESHRIFELVKEIDTSNQDASKSEKILQQRLDLLHQVRLRYFTPREVARLMSFPENFEFPPETTNRQKYRLLGNSINVKVVGELIKLL 342
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Zebrafish
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:ZFIN;Acc:ZDB-GENE-041008-138])

HSP 1 Score: 223.016 bits (567), Expect = 7.132e-68
Identity = 153/379 (40.37%), Postives = 210/379 (55.41%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNF-TSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQV--RPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKG-SLMQDLEAISYKPY---FDRKLYQCNCPVCSGRSRS------LENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHK--LIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGD-ISSLD---YAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLIS 1117
            ++V E +SGIGG+  AL E  +  +VVAA D+N  AN +Y++NF T+    K IE ++ +    LN DM  MSPPCQP+TR G   D+ DPRT +  + L L+ ++  RP +I  ENVKGFESS  +  L+  L E  YSFQEFL+SP   G+PNSRLRY+LIAK   +  S     E I   P     D      + P  S   R       LE +     ER  +   + S     D L E  E  +  L+  K L +    +DIV  SS ++ CF K Y   +EG+GS  Q  SC +   L+ +  N D +S  D      RLKLRYF+PREI+  M FP  F FP+ ++  Q+YR+LGNS+NV VV+H++  ++S
Sbjct:    7 LRVFELYSGIGGMHYALKESLVPAEVVAAVDVNTTANLIYKHNFPTTQLLPKTIEGMTLQDFDRLNFDMILMSPPCQPFTRIGLQGDVADPRTKSFLYILDLLPRLSKRPRFILLENVKGFESSAARDALLQTLRECDYSFQEFLISPTSLGIPNSRLRYFLIAKRAPETFSFPVSTEIIEGFPMSESTDGLSVPYDHPTSSASEREKTIMFKLETEE--DLERKRKQNSQESVRRLLDFLQEEEEDMEPYLLPPKTLLRYALVMDIVQPSSRRSVCFTKGYGHYVEGTGSVLQ--SCMDV-DLETVFKNLDQLSEEDKLKQLLRLKLRYFTPREISRLMGFPDHFTFPKHISFKQQYRVLGNSLNVHVVSHLIRLMLS 380
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Match: mapk15 (mitogen-activated protein kinase 15 [Source:Xenbase;Acc:XB-GENE-1010338])

HSP 1 Score: 227.254 bits (578), Expect = 3.777e-69
Identity = 147/394 (37.31%), Postives = 219/394 (55.58%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK----GSLMQDLEAISYKPYFDRKLYQC--------------NCPVCSGRSRSLENDHVNHFERNLEFCDR-----------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            ++V+E +SGIGG+ C L E  +  +VVAA D+N +AN VY+YNF  +P + K IE +S + L +L+ DM  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE+S  ++ L+  L +  Y++QEFLLSP   G+PNSRLRY+LIAKL+ +     +  + LE    +    +++  C              +CP+ SG     E   +   E  +E   +           +  +LE  N+ E       +  K L +    LDIV  +  ++ CF K Y   +EG+GS  Q  +  E   + K L L  +   L     LK+RYF+PREI+N   FP +F FPE VT+ QRYRLLGNS+NV +V+ ++  L+ +
Sbjct:    5 LRVLELYSGIGGMHCGLTESGVAAEVVAAVDVNTIANEVYKYNFPYTPLWPKSIEGLSLKELDALSFDMILMSPPCQPFTRIGLQGDISDPRTKSFLYVLDVLPRLKKQPAYILLENVKGFETSEAREALIRTLEKGGYAYQEFLLSPTCLGIPNSRLRYFLIAKLQKEPFAFPTTSKILEEFPSQCPGSKRIIHCSESNPQARVEQKYSSCPL-SGTDCDPEKTVLYKLETAVELQRKQTQDNDSSVRMLQDFLEG-NVEE--MSQYFLPPKSLLRYALILDIVKPTCRRSTCFTKGYGHYVEGTGSVLQTATDVEIDTVYKSLDLLTEEEKLAKLSSLKMRYFTPREISNLHGFPATFGFPEEVTKKQRYRLLGNSLNVHIVSCLISTLLQS 394
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Match: mapk15 (mitogen-activated protein kinase 15 [Source:Xenbase;Acc:XB-GENE-1010338])

HSP 1 Score: 210.69 bits (535), Expect = 3.196e-63
Identity = 140/366 (38.25%), Postives = 203/366 (55.46%), Query Frame = 2 
Query:   74 IKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQ--CNCPVCSGRSRSLENDHVNHFERNLEFCDR-----------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            + C L E  +  +VVAA D+N +AN VY+YNF  +P + K IE +S + L +L+ DM  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE+S  ++ L+  L +  Y++QEFLLSP   G+PNSRLRY+LIAKL+ K        + +        L Q   +CP+ SG     E   +   E  +E   +           +  +LE  N+ E       +  K L +    LDIV  +  ++ CF K Y   +EG+GS  Q  +  E   + K L L  +   L     LK+RYF+PREI+N   FP +F FPE VT+ QRYRLLGNS+NV +V+ ++  L+ +
Sbjct:    1 MHCGLTESGVAAEVVAAVDVNTIANEVYKYNFPYTPLWPKSIEGLSLKELDALSFDMILMSPPCQPFTRIGLQGDISDPRTKSFLYVLDVLPRLKKQPAYILLENVKGFETSEAREALIRTLEKGGYAYQEFLLSPTCLGIPNSRLRYFLIAKLQ-KEPFAFPTTSKTRPEILSVDLIQKYSSCPL-SGTDCDPEKTVLYKLETAVELQRKQTQDNDSSVRMLQDFLEG-NVEE--MSQYFLPPKSLLRYALILDIVKPTCRRSTCFTKGYGHYVEGTGSVLQTATDVEIDTVYKSLDLLTEEEKLAKLSSLKMRYFTPREISNLHGFPATFGFPEEVTKKQRYRLLGNSLNVHIVSCLISTLLQS 361
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Match: mapk15 (mitogen-activated protein kinase 15 [Source:Xenbase;Acc:XB-GENE-1010338])

HSP 1 Score: 206.453 bits (524), Expect = 2.189e-61
Identity = 139/382 (36.39%), Postives = 208/382 (54.45%), Query Frame = 2 
Query:   74 IKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK----GSLMQDLEAISYKPYFDRKLYQC--------------NCPVCSGRSRSLENDHVNHFERNLEFCDR-----------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            + C +  L +  +VVAA D+N +AN VY+YNF  +P + K IE +S + L +L+ DM  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE+S  ++ L+  L +  Y++QEFLLSP   G+PNSRLRY+LIAKL+ +     +  + LE    +    +++  C              +CP+ SG     E   +   E  +E   +           +  +LE  N+ E       +  K L +    LDIV  +  ++ CF K Y   +EG+GS  Q  +  E   + K L L  +   L     LK+RYF+PREI+N   FP +F FPE VT+ QRYRLLGNS+NV +V+ ++  L+ +
Sbjct:    1 VHCNILNLGVAAEVVAAVDVNTIANEVYKYNFPYTPLWPKSIEGLSLKELDALSFDMILMSPPCQPFTRIGLQGDISDPRTKSFLYVLDVLPRLKKQPAYILLENVKGFETSEAREALIRTLEKGGYAYQEFLLSPTCLGIPNSRLRYFLIAKLQKEPFAFPTTSKILEEFPSQCPGSKRIIHCSESNPQARVEQKYSSCPL-SGTDCDPEKTVLYKLETAVELQRKQTQDNDSSVRMLQDFLEG-NVEE--MSQYFLPPKSLLRYALILDIVKPTCRRSTCFTKGYGHYVEGTGSVLQTATDVEIDTVYKSLDLLTEEEKLAKLSSLKMRYFTPREISNLHGFPATFGFPEEVTKKQRYRLLGNSLNVHIVSCLISTLLQS 378
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Match: mapk15 (mitogen-activated protein kinase 15 [Source:Xenbase;Acc:XB-GENE-1010338])

HSP 1 Score: 206.068 bits (523), Expect = 2.912e-61
Identity = 132/362 (36.46%), Postives = 196/362 (54.14%), Query Frame = 2 
Query:  134 NDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK----GSLMQDLEAISYKPYFDRKLYQC--------------NCPVCSGRSRSLENDHVNHFERNLEFCDR-----------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            N +AN VY+YNF  +P + K IE +S + L +L+ DM  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE+S  ++ L+  L +  Y++QEFLLSP   G+PNSRLRY+LIAKL+ +     +  + LE    +    +++  C              +CP+ SG     E   +   E  +E   +           +  +LE  N+ E       +  K L +    LDIV  +  ++ CF K Y   +EG+GS  Q  +  E   + K L L  +   L     LK+RYF+PREI+N   FP +F FPE VT+ QRYRLLGNS+NV +V+ ++  L+ +
Sbjct:   23 NTIANEVYKYNFPYTPLWPKSIEGLSLKELDALSFDMILMSPPCQPFTRIGLQGDISDPRTKSFLYVLDVLPRLKKQPAYILLENVKGFETSEAREALIRTLEKGGYAYQEFLLSPTCLGIPNSRLRYFLIAKLQKEPFAFPTTSKILEEFPSQCPGSKRIIHCSESNPQARVEQKYSSCPL-SGTDCDPEKTVLYKLETAVELQRKQTQDNDSSVRMLQDFLEG-NVEE--MSQYFLPPKSLLRYALILDIVKPTCRRSTCFTKGYGHYVEGTGSVLQTATDVEIDTVYKSLDLLTEEEKLAKLSSLKMRYFTPREISNLHGFPATFGFPEEVTKKQRYRLLGNSLNVHIVSCLISTLLQS 380
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Match: mapk15 (mitogen-activated protein kinase 15 [Source:Xenbase;Acc:XB-GENE-1010338])

HSP 1 Score: 186.422 bits (472), Expect = 3.373e-54
Identity = 118/322 (36.65%), Postives = 176/322 (54.66%), Query Frame = 2 
Query:  191 VIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK----GSLMQDLEAISYKPYFDRKLYQC--NCPVCSGRSRSLEND---HVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            ++  +S + L +L+ DM  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE+S  ++ L+  L +  Y++QEFLLSP   G+PNSRLRY+LIAKL+ +     +  + LE    +    +++  C  NC         LE          + N      +  +LE  N+ E       +  K L +    LDIV  +  ++ CF K Y   +EG+GS  Q  +  E   + K L L  +   L     LK+RYF+PREI+N   FP +F FPE VT+ QRYRLLGNS+NV +V+ ++  L+ +
Sbjct:   19 LLSGLSLKELDALSFDMILMSPPCQPFTRIGLQGDISDPRTKSFLYVLDVLPRLKKQPAYILLENVKGFETSEAREALIRTLEKGGYAYQEFLLSPTCLGIPNSRLRYFLIAKLQKEPFAFPTTSKILEEFPSQCPGSKRIIHCSENCDPEKTVLYKLETAVELQRKQTQDNDSSVRMLQDFLEG-NVEE--MSQYFLPPKSLLRYALILDIVKPTCRRSTCFTKGYGHYVEGTGSVLQTATDVEIDTVYKSLDLLTEEEKLAKLSSLKMRYFTPREISNLHGFPATFGFPEEVTKKQRYRLLGNSLNVHIVSCLISTLLQS 337
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Mouse
Match: Trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:MGI Symbol;Acc:MGI:1274787])

HSP 1 Score: 230.72 bits (587), Expect = 2.143e-70
Identity = 149/391 (38.11%), Postives = 214/391 (54.73%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKG--------SLMQDLEAISYKPY-------FDRKLYQCNCPVC--SGRSRSLENDHVNHFERNLEFCDR-------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAH------QLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++V+E +SGIGG+  AL E +I   VVAA D+N +AN VY++NF  +   +K IE IS E    L+ +M  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +          LM+  + ++ +P           ++ +    +C  S  ++S   D +      +E  DR       +S  +  D L +      L+  K+L +    LDIV  +S ++ CF K Y   IEG+GS  Q  + ++A        L  L     I+ L     LKLRYF+P+EIAN   FP  F FPE  T  QRYRLLGNS+NV VVA +L  L
Sbjct:    4 LRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNFPHTHLLSKTIEGISLEDFDKLSFNMILMSPPCQPFTRIGLQGDMTDPRTTSFLYILDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTIEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPFPFQAPGQILMEFPKIVTVEPQKYAVVEESQPRVQRTGPRICAESSSTQSSGKDTILFKLETVEERDRKHQQDSDLSVQMLKDFLEDGDTDEYLLPPKLLLRYALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQ--AAEDAQIENIYKSLPDLPPEEKIAKLSM---LKLRYFTPKEIANLQGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVAKLLTVL 389
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Mouse
Match: Trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:MGI Symbol;Acc:MGI:1274787])

HSP 1 Score: 77.7962 bits (190), Expect = 2.470e-17
Identity = 39/80 (48.75%), Postives = 54/80 (67.50%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYT 274
            ++V+E +SGIGG+  AL E +I   VVAA D+N +AN VY++NF  +   +K IE IS E    L+ +M  MSPPCQP+T
Sbjct:    4 LRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNFPHTHLLSKTIEGISLEDFDKLSFNMILMSPPCQPFT 83
[Close]
BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Match: sp|O14717|TRDMT_HUMAN (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 7.005e-71
Identity = 146/398 (36.68%), Postives = 216/398 (54.27%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK-------GSLMQD---LEAISYKPY---FDRKLYQCNCPV---------CSGRSRSL-ENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHK-LIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGS-------------YYQMTSCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++V+E +SG+GG+  AL E  I   VVAA D+N +AN VY+YNF  +    K IE I+ E    L+ DM  MSPPCQP+TR G   D+ D RT +  H L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +       G ++ +   +E++  + Y    + K+ + N            CSG+   L + +      R  +    +S  +  D L +  + ++ L+  K L +    LDIV  +  ++ CF K Y   IEG+GS             Y  +T+  +  Q+ +LL+            LKLRYF+P+EIAN + FP  F FPE +T  QRYRLLGNS+NV VVA ++  L
Sbjct:    4 LRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLHILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLI------------LKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKIL 389
[Close]
BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Match: sp|O55055|TRDMT_MOUSE (tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE=1 SV=2)

HSP 1 Score: 230.72 bits (587), Expect = 1.499e-69
Identity = 149/391 (38.11%), Postives = 214/391 (54.73%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKG--------SLMQDLEAISYKPY-------FDRKLYQCNCPVC--SGRSRSLENDHVNHFERNLEFCDR-------ISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAH------QLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++V+E +SGIGG+  AL E +I   VVAA D+N +AN VY++NF  +   +K IE IS E    L+ +M  MSPPCQP+TR G   D+ DPRT +  + L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +          LM+  + ++ +P           ++ +    +C  S  ++S   D +      +E  DR       +S  +  D L +      L+  K+L +    LDIV  +S ++ CF K Y   IEG+GS  Q  + ++A        L  L     I+ L     LKLRYF+P+EIAN   FP  F FPE  T  QRYRLLGNS+NV VVA +L  L
Sbjct:    4 LRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNFPHTHLLSKTIEGISLEDFDKLSFNMILMSPPCQPFTRIGLQGDMTDPRTTSFLYILDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTIEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPFPFQAPGQILMEFPKIVTVEPQKYAVVEESQPRVQRTGPRICAESSSTQSSGKDTILFKLETVEERDRKHQQDSDLSVQMLKDFLEDGDTDEYLLPPKLLLRYALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQ--AAEDAQIENIYKSLPDLPPEEKIAKLSM---LKLRYFTPKEIANLQGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVAKLLTVL 389
[Close]
BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Match: sp|Q7YS61|TRDMT_BOVIN (tRNA (cytosine-5-)-methyltransferase OS=Bos taurus OX=9913 GN=TRDMT1 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 3.448e-69
Identity = 143/386 (37.05%), Postives = 207/386 (53.63%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLE-------GKGSLMQDLEAIS--YKPYF---------DRKLYQCNC----PVCSGRSRSL-ENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKL-IDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++ +E +SGIGG+  AL E  I   VVAA D+N +AN VY+YNF  +    K IE I+ E    L+ +M  MSPPCQP+TR G   D+ DPRT +  H L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+         G ++ +       + P +         ++K     C      CSG+   L + +     +R  +    +S  +  D L +  + H   +  K L +    LDIV  +S ++ CF K Y + IEG+GS  Q T   +   + K L        +     L+LR+F+P+EIAN + FP  F FPE  T  QRYRLLGNS+NV VVA ++  L
Sbjct:    4 LRALELYSGIGGMHQALRESCIPAQVVAAVDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFNMILMSPPCQPFTRIGLQGDVTDPRTNSFLHILDILPRLQKLPKYILLENVKGFEMSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQPEPFPFQAPGQVLMEFPKTESEHPPKYAINAEKKTEEKKTGPKICFDSSTQCSGKEAILFKLETAGEIDRKHQQDSDLSVRMLKDFLEDDIDKHSFFLPPKSLLRYALLLDIVKPTSRRSMCFTKGYGRYIEGTGSVLQTTEDVQIENIYKSLTSLSQEEKIMRLSMLQLRFFTPKEIANLLGFPPEFGFPEMTTVKQRYRLLGNSLNVHVVAKLIKIL 389
[Close]
BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Match: sp|Q54JH6|CMT1_DICDI (DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA PE=1 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 5.373e-67
Identity = 146/369 (39.57%), Postives = 201/369 (54.47%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFE---SSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLEN-DHVN--HFERNLEFCDRISAYLEAD-NLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLIS 1117
            ++V+EF+SGIGG+   L E  +   V+ +FDIN  AN  Y+Y F      K IES S E L    A+ W MSPPCQP+TR G   D  D RT +  H L ++++++  P YI  ENV GF    SSN +  L+  L +  YSFQEF LSP QFG+ N RLRY+ IAK  GK +  ++ +  + K   ++     N      +  +L+  DH+    F   LE CD IS Y + D    E +E +K+    +L KG    DI  + S  + C  K Y K IEG+GS  QM +  +A          DI+       LKLRYFSP+EI     FP+ F+F   +T  Q YRL+GNS+NVK+V+ +L  L+S
Sbjct:    4 LRVLEFYSGIGGMHYGLQESGVDFQVIQSFDINTNANLNYKYTFNEDSSQKSIESYSVEELEGFKANAWLMSPPCQPFTRLGLQKDDQDNRTNSFFHLLDVLTKIKDPPTYILIENVFGFAKKGSSNTRDHLLDTLIKMNYSFQEFHLSPQQFGLANQRLRYFCIAKRNGKLNFKKEQDKHNEKVDENKLNNNSNNNNEQNKYDNLKILDHIPGYDFHTTLEECDEISNYFDKDLTDDELYEKYKVPHNLLLSKGML-FDIKQKDSKTSNCVTKSYGKFIEGTGSIIQMDNNFKA----------DINDNKSLIPLKLRYFSPKEITRLHGFPEEFKFSPKLTTIQCYRLIGNSLNVKIVSELLKVLVS 361
[Close]
BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Match: sp|Q4G073|TRDMT_RAT (tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdmt1 PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 3.267e-66
Identity = 142/389 (36.50%), Postives = 206/389 (52.96%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK-------GSLMQD------LEAISYKPYFDRKL----------YQCNCPVCSGRSRSL-ENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAH----QLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWL 1111
            ++V+E +SGIGG+  AL E  +   VVAA D++ +AN VY++NF  +    K IE IS E    L+ +M  MSPPCQP+TR G   D+ D RT +  + L ++ +++  P YI  ENVKGFE S+ + +L+  +    + +QEFLLSP   G+PNSRLRY+LIAKL+ +       G ++ +      ++   Y    + KL             +   CSG+   L +++     +R  +    +S  +    L +      L+  K L +    LDIV  +S ++ CF K Y   IEG+GS  Q     +       L  L     I+ L     LKLRYF+P+EIAN + FP  F FPE  T  QRYRLLGNS+NV VV+ +L  L
Sbjct:    4 LRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNFPHTHLLAKTIEGISLEEFDKLSFNMILMSPPCQPFTRIGLQGDMSDRRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTMEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPLCFQAPGQILMEFPNSGTVQPQEYAVVEEGKLRVRTREPDVCLDSSSTQCSGQDSILFKHETAADIDRKRQQDSDLSVQMLKGFLEDGDTAQYLLPAKSLLRYALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQTAEDVQIENIYKSLPDLPPEEKIAKLSM---LKLRYFTPKEIANLLGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVSKLLTVL 389
[Close]
BLAST of DNA methyl-transferase vs. TrEMBL
Match: V5UWJ2 (DNA methyl-transferase OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0
Identity = 293/294 (99.66%), Postives = 294/294 (100.00%), Query Frame = 2 
Query:  239 MWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            MWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSC+EAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA
Sbjct:    1 MWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCEEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 294
[Close]
BLAST of DNA methyl-transferase vs. TrEMBL
Match: A0A4E0QTC6 (tRNA aspartic acid methyltransferase 1 OS=Fasciola hepatica OX=6192 GN=D915_009706 PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 4.756e-92
Identity = 160/372 (43.01%), Postives = 222/372 (59.68%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIA----------KLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMT----SCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHW 1108
            M+V+E +SGIGGI CAL +  +   +  A D+NDLA+SVYR+NF  SP  N+VIE +S E +  ++AD+WTMSPPCQP+TR GN  D  D R+V+  H L LI+ +RP +IF ENV+GFESS   + L+  L  + Y +Q+FLL+PLQFG+PN RLR+YL+A          K +     + D   I   P  D     C C VC+G  R++  ++ N F   L +C  ++ ++ +  L E  E    I    L++ F  +DIV     KT CF K Y K+IEG+GS  Q +    + D  H   Q + +   + + + + L LR+F  RE+AN MCFP  + FPE VT  QR RLLGNSVNV VVAH++ W
Sbjct:    1 MRVLELYSGIGGIHCALKKSKLSYSIRIAIDVNDLASSVYRHNFPQSPASNRVIEGLSIEEVSRMSADLWTMSPPCQPFTRLGNRRDEADNRSVSFFHVLTLIAAIRPSFIFLENVRGFESSESCRELLKTLDSNEYLYQQFLLTPLQFGIPNCRLRFYLVAWRGPTCTNPFKTDDSSLCILDTPPIHLPPLPD-----CVCSVCNGCVRNVTANNDN-FSEYLPYCQPVANFVHS--LNEHEEKSCSITSDCLKRYFYVMDIVRPCDRKTRCFTKGYTKRIEGTGSVLQTSRLDLTSDLIHSNYQSVEHDSDALVSFTRTLGLRFFHSREVANLMCFPSDYEFPENVTEKQRIRLLGNSVNVHVVAHLMFW 364
[Close]
BLAST of DNA methyl-transferase vs. TrEMBL
Match: C4Q8S0 (DNA-methyltransferase 2 isoform 1 OS=Schistosoma mansoni OX=6183 GN=Dnmt2 PE=2 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.380e-91
Identity = 160/370 (43.24%), Postives = 226/370 (61.08%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSP-CYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGS----LMQDLEAISYKPYFDRKLYQ-CNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHK---LIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQ----LKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHW 1108
            M+V+E +SGIGG+  A  E  ++ +VVAA +IN +A  VY+YNF +    N+VIES S +Y+ SLNA++W++ PPCQP+TR G  M   D R+ +  H L LIS ++P  I  ENVKGFE S   + L+ +L+   Y +++FLLSPLQFG+PN RLR+YL+A+L          M   E+I  +P  D  +   C C  CSG    +E+   N F   ++FC  IS +L       P +  K    +DEK L++ F  LDIV     KT CF K Y+K++EG+GS +Q +  +E  +      +     + + L YAK LKLR+F  RE+AN MCFP+SF FPE +T  QR RLLGNSVN+ VV+H+++W
Sbjct:    1 MRVLELYSGIGGMHIAFKESTVKHEVVAAVEINGVATDVYKYNFPNTLTLNRVIESFSPDYVCSLNANVWSLCPPCQPFTRLGKRMCEADNRSSSFFHVLDLISILKPAGIILENVKGFEHSEPWRRLIEVLNSCDYEYRQFLLSPLQFGIPNCRLRFYLLARLRSSSWNSNFKMGKSESIDLRPPIDAPMLPGCQCTSCSGVISHIEHTDDN-FTEYIQFCRPISEFLLV-----PSDSSKELYFLDEKCLQRYFRVLDIVRSCDKKTRCFTKGYSKRLEGTGSVFQTSMENETSEKITNFYEANKEDEQAVLQYAKLLKLRFFHSREVANMMCFPKSFDFPEHITEKQRLRLLGNSVNILVVSHLIYW 364
[Close]
BLAST of DNA methyl-transferase vs. TrEMBL
Match: A0A504YBD6 (tRNA aspartic acid methyltransferase 1 OS=Fasciola gigantica OX=46835 GN=FGIG_04544 PE=3 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 3.797e-90
Identity = 160/373 (42.90%), Postives = 221/373 (59.25%), Query Frame = 2 
Query:   23 NIFL*MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNF-TSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLM------QDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMT----SCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHW 1108
            N F  M+V+E +SGIGGI CAL +  +   +  A D+NDLA+SVYR+NF  SP  N+VIE +S E +  ++AD+WTMSPPCQP+TR GN  +  D R+V+  H L LI+ +RP +IF ENV+GFESS   + L+  L  + Y +Q+FLL+PLQFG+PN RLR+YL+A   G             L  +   P     L  C C VC G  R++  ++ N F   L +C  ++ ++ +  L E  E    I    L++ F  +DIV     KT CF K Y K+IEG+GS  Q +    + D  H   Q + +   + + + + L LR+F  RE+AN MCFP  + FPE VT  QR RLLGNSVNV VVAH++ W
Sbjct:   22 NSFREMRVLELYSGIGGIHCALKKSKLSYSIRIAIDVNDLASSVYRHNFPQSPVSNRVIEGLSIEEVSRMSADLWTMSPPCQPFTRLGNRRNEADNRSVSFFHVLTLIAAIRPSFIFLENVRGFESSESCRELLKTLDSNEYLYQQFLLTPLQFGIPNCRLRFYLVA-WRGPSCTNPFKTNDSSLCILDTPPIHLPSLPDCVCSVCKGCVRNVTANNDN-FSEYLPYCQPVANFVHS--LNEHEEKSCSITSDCLKRYFYVMDIVRPCDRKTRCFTKGYTKRIEGTGSVLQTSRLDLTSDLIHSNYQSVEHDSDALVSFTRTLGLRFFHSREVANLMCFPSEYEFPENVTEKQRIRLLGNSVNVHVVAHLMFW 390
[Close]
BLAST of DNA methyl-transferase vs. TrEMBL
Match: A0A4S2LVL8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004544 PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 3.934e-90
Identity = 164/380 (43.16%), Postives = 212/380 (55.79%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSP-CYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLM----------QDLEAISYKPYFD-RKLYQCNCPVCSGRSRSLENDHVNH-------FERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQM---TSCDEAHQLKQLLLNGDISSL-DYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHW 1108
            M+V+E +SGIGG+ CA     +   VVAA DIND AN VYR NF+     N+V+ES+S E + + N DMWTMSPPCQP+TR GN    +D R+ +  + L LI+ +RP +I  ENVKGFE +      + +L    Y +Q+FLL+PLQFGVPN RLRYYL+A      +                 I   P  D   L  C CPVC G        HVNH       F   L +C  IS YL      + H     +D+  L++ F  LDIV     K+ CF K Y K+IEG+GS  Q    T   E    + +    D  +L   AKRL+LR+F  RE+AN +CFPQ++ FPE VT  QR RLLGNS+NV VVAH++HW
Sbjct:    1 MRVLELYSGIGGMHCAFKMAQLDFQVVAAIDINDTANRVYRANFSHTYAPNRVLESLSIEEVTAFNVDMWTMSPPCQPFTRLGNQKHEEDNRSTSFFYVLGLIAAIRPKFILLENVKGFEHTEPWGQFLKVLQTCGYRYQQFLLTPLQFGVPNCRLRYYLVASSSSDSASSGLFAPHVHDSNHANTIHLIPPADLPPLPGCECPVCLG--------HVNHIIKPDENFNDYLPYCRPISDYLAQH---QQHSNFDFLDDSCLKRYFHVLDIVRPRDRKSRCFTKGYQKRIEGTGSVLQTETDTLTSEEINAQWITARSDPETLMSLAKRLQLRFFHSREVANLLCFPQTYTFPEDVTEKQRIRLLGNSINVLVVAHLIHW 369
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Cavefish
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:NCBI gene;Acc:103043215])

HSP 1 Score: 236.498 bits (602), Expect = 5.110e-73
Identity = 144/382 (37.70%), Postives = 206/382 (53.93%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCY-NKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLE-GKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNH------------FERNL-----EFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLIS 1117
            ++V+E +SGIGG+  AL E ++  +VVAA D+N  AN +Y++NF +     K IE ++      L+ DM  MSPPCQP+TR G   D+ DPRT +  + L L+ ++   P +I  ENVKGFE+S+ +  L+  L E  YSFQE ++SP   G+PNSRLRY+LIAK   G  S     E +   P            + +  S SL ++  ++             ER       E   R+  +LE +   E    H L+  K L +    +DIV+ +  ++ CF K Y   +EG+GS  Q  S  E   + K L    +   L    RLKLRYF+PREIAN M FP  F FP  ++  Q+YR+LGNS+NV VVA ++  L+S
Sbjct:    4 LRVLELYSGIGGMHYALTESSVPAEVVAAVDVNTTANEIYKHNFPNTLLLPKTIEGMTLNDFNKLDFDMILMSPPCQPFTRIGLQGDVSDPRTKSFLYILELLPRLNKLPRFILLENVKGFETSSARDALIKTLKECEYSFQELMVSPTSLGIPNSRLRYFLIAKASTGSLSFQTTSEILEGFPKTSSSDATGTPTISNTHSASLSSNMSDNKDIIYKLETAQDLERKRSQNSEESIRRLQDFLEEEG-QETDMDHYLLPPKTLLRYAMLMDIVSPTCRRSVCFTKGYGHYVEGTGSVLQSCSDVELESVFKSLEGLSEEEKLKQLSRLKLRYFTPREIANLMGFPPHFTFPPNISIKQQYRVLGNSLNVHVVAKLISLLVS 384
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Cavefish
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:NCBI gene;Acc:103043215])

HSP 1 Score: 209.149 bits (531), Expect = 7.574e-63
Identity = 133/368 (36.14%), Postives = 194/368 (52.72%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCY-NKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQF--GVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRK--LYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLIS 1117
            ++V+E +SGIGG+  AL E ++  +VVAA D+N  AN +Y++NF +     K IE ++      L+ DM  MSPPCQP+TR G   D+ DPRT +  + L L+ ++   P +I  ENVKGFE+S+ +  L+  L E  YSFQE ++SP     G P +       +   G  ++     A       D K  +Y+        R RS  ++         E   R+  +LE +   E    H L+  K L +    +DIV+ +  ++ CF K Y   +EG+GS  Q  S  E   + K L    +   L    RLKLRYF+PREIAN M FP  F FP  ++  Q+YR+LGNS+NV VVA ++  L+S
Sbjct:    4 LRVLELYSGIGGMHYALTESSVPAEVVAAVDVNTTANEIYKHNFPNTLLLPKTIEGMTLNDFNKLDFDMILMSPPCQPFTRIGLQGDVSDPRTKSFLYILELLPRLNKLPRFILLENVKGFETSSARDALIKTLKECEYSFQELMVSPTSILEGFPKTS-----SSDATGTPTISNTHSASLSSNMSDNKDIIYKLETAQDLERKRSQNSE---------ESIRRLQDFLEEEG-QETDMDHYLLPPKTLLRYAMLMDIVSPTCRRSVCFTKGYGHYVEGTGSVLQSCSDVELESVFKSLEGLSEEEKLKQLSRLKLRYFTPREIANLMGFPPHFTFPPNISIKQQYRVLGNSLNVHVVAKLISLLVS 356
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Cavefish
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:NCBI gene;Acc:103043215])

HSP 1 Score: 164.851 bits (416), Expect = 4.805e-46
Identity = 105/315 (33.33%), Postives = 160/315 (50.79%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCY-NKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLE-GKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNH------------FERNL-----EFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKK------IEGSGSYYQMTSCDEAHQ 901
            ++V+E +SGIGG+  AL E ++  +VVAA D+N  AN +Y++NF +     K IE ++      L+ DM  MSPPCQP+TR G   D+ DPRT +  + L L+ ++   P +I  ENVKGFE+S+ +  L+  L E  YSFQE ++SP   G+PNSRLRY+LIAK   G  S     E +   P            + +  S SL ++  ++             ER       E   R+  +LE +   E    H L+  K L +    +DIV+ +  ++ CF K ++ K      + G G   + +   + HQ
Sbjct:    4 LRVLELYSGIGGMHYALTESSVPAEVVAAVDVNTTANEIYKHNFPNTLLLPKTIEGMTLNDFNKLDFDMILMSPPCQPFTRIGLQGDVSDPRTKSFLYILELLPRLNKLPRFILLENVKGFETSSARDALIKTLKECEYSFQELMVSPTSLGIPNSRLRYFLIAKASTGSLSFQTTSEILEGFPKTSSSDATGTPTISNTHSASLSSNMSDNKDIIYKLETAQDLERKRSQNSEESIRRLQDFLEEEG-QETDMDHYLLPPKTLLRYAMLMDIVSPTCRRSVCFTKGFSTKYLHKAAVPGPGKQSKCSCSGQTHQ 317
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Nematostella
Match: EDO32554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUR9])

HSP 1 Score: 120.553 bits (301), Expect = 1.408e-31
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 2 
Query:   41 KVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNF-TSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAK 523
            +V+EF+SGIGG+  AL       +VVAA +I+  AN+VY +NF T+  +N  IE      + ++ A    MSPPCQPYT  G      DPR ++  H L L+ +++  P Y   ENVKGFE+S+ +  ++     S      F++S  QFG+PNSRLRYYL+AK
Sbjct:    8 RVVEFYSGIGGMHYALKGCKKNAEVVAALEISTTANTVYGHNFPTTKIWNCNIEVCELCNVTTMPAIYMVMSPPCQPYTWVGLQGASKDPRALSFLHILSLLKRLQHPPKYWLIENVKGFETSDTRFYILLAFCNS------FIVSSPQFGIPNSRLRYYLLAK 165
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Medaka
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:NCBI gene;Acc:101155097])

HSP 1 Score: 250.366 bits (638), Expect = 4.637e-78
Identity = 149/385 (38.70%), Postives = 214/385 (55.58%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK------GSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDH----VNHFERNLEF-----------CDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLIS 1117
            ++V+E +SGIGG+  AL E  +  +VVAA DIN +AN +Y++NF  +P +NK IE ++ E    L+ DM  MSPPCQP+TR G   D+ DPRT +  H L+L+ ++   P +I  ENVKGFE+S  +  L++ L+E  Y FQE + SP   G+PNSRLRY+LIA+   +      GS + D+ A   +P  D  L   +   CSG +   E       +   E  ++              +I  +LEA +     E   L+  K L +    LDIV  +S ++ CF K Y + +EG+GS  Q     E   +   L L  +   L     L+LRYF+PRE+AN MCFP  F FPE ++  QRYR+LGNS+NV +VA ++  L S
Sbjct:    4 VRVLELYSGIGGMHYALKESGVPAEVVAAVDINTMANQIYKHNFPDTPLWNKSIEGLTLEDFAGLSVDMVMMSPPCQPFTRIGLQGDVSDPRTKSFLHILHLLPRLHRPPRFILLENVKGFETSAARDRLINTLTECGYVFQEIMASPTSVGIPNSRLRYFLIARRSAENMNIHTGSKVWDVCA---QPAED-DLALVSKAACSGTTPPEEKASHGLVLYKLETEMQLQRKTTQNSNPDVKQIQDFLEAGSQVNVEE--YLLPPKTLLRYSLLLDIVQPTSRRSVCFTKGYGRYVEGTGSVLQGCMDTEVQSVFTDLELLSEEDRLQRLMSLRLRYFTPREVANLMCFPPCFSFPEHISTIQRYRVLGNSLNVLLVAKLVQLLFS 382
[Close]
BLAST of DNA methyl-transferase vs. Ensembl Medaka
Match: trdmt1 (tRNA aspartic acid methyltransferase 1 [Source:NCBI gene;Acc:101155097])

HSP 1 Score: 215.312 bits (547), Expect = 2.074e-65
Identity = 132/352 (37.50%), Postives = 190/352 (53.98%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFT-SPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVR--PHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGK------GSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDH----VNHFERNLEF-----------CDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQL-KQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSF 1018
            ++V+E +SGIGG+  AL E  +  +VVAA DIN +AN +Y++NF  +P +NK IE ++ E    L+ DM  MSPPCQP+TR G   D+ DPRT +  H L+L+ ++   P +I  ENVKGFE+S  +  L++ L+E  Y FQE + SP   G+PNSRLRY+LIA+   +      GS + D+ A   +P  D  L   +   CSG +   E       +   E  ++              +I  +LEA +     E   L+  K L +    LDIV  +S ++ CF K Y + +EG+GS  Q     E   +   L L  +   L     L+LRYF+PRE+AN MCFP  F
Sbjct:    4 VRVLELYSGIGGMHYALKESGVPAEVVAAVDINTMANQIYKHNFPDTPLWNKSIEGLTLEDFAGLSVDMVMMSPPCQPFTRIGLQGDVSDPRTKSFLHILHLLPRLHRPPRFILLENVKGFETSAARDRLINTLTECGYVFQEIMASPTSVGIPNSRLRYFLIARRSAENMNIHTGSKVWDVCA---QPAED-DLALVSKAACSGTTPPEEKASHGLVLYKLETEMQLQRKTTQNSNPDVKQIQDFLEAGSQVNVEE--YLLPPKTLLRYSLLLDIVQPTSRRSVCFTKGYGRYVEGTGSVLQGCMDTEVQSVFTDLELLSEEDRLQRLMSLRLRYFTPREVANLMCFPPCF 349
[Close]
BLAST of DNA methyl-transferase vs. Planmine SMEST
Match: SMESG000017337.1 (SMESG000017337.1)

HSP 1 Score: 746.503 bits (1926), Expect = 0.000e+0
Identity = 355/361 (98.34%), Postives = 360/361 (99.72%), Query Frame = 2 
Query:   38 MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLYLISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISSLDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 1120
            MKVIEFFSGIGGIKCALNELN+QTDVVAAFDINDLANSVYRYNFTSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRT AMKHTLYLISQVRPHYIFFENVKGFESSNG+KMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKG+LMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSC+EAHQLKQLLLNGDISSLDYAKRL+LRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA
Sbjct:    1 MKVIEFFSGIGGIKCALNELNVQTDVVAAFDINDLANSVYRYNFTSPCYNKVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTAAMKHTLYLISQVRPHYIFFENVKGFESSNGRKMLVSILSESAYSFQEFLLSPLQFGVPNSRLRYYLIAKLEGKGNLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRSLENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKLDIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCEEAHQLKQLLLNGDISSLDYAKRLQLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKVVAHVLHWLISA 361
The following BLAST results are available for this feature:
BLAST of DNA methyl-transferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRDMT11.459e-7136.68tRNA aspartic acid methyltransferase 1 [Source:HGN... [more]
TRDMT12.256e-3244.08tRNA aspartic acid methyltransferase 1 [Source:HGN... [more]
TRDMT17.750e-2035.62tRNA aspartic acid methyltransferase 1 [Source:HGN... [more]
TRDMT17.145e-952.27tRNA aspartic acid methyltransferase 1 [Source:HGN... [more]
TRDMT11.543e-852.27tRNA aspartic acid methyltransferase 1 [Source:HGN... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA methyl-transferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mt25.355e-7036.02gene:FBgn0028707 transcript:FBtr0110911[more]
Mt25.355e-7036.02gene:FBgn0028707 transcript:FBtr0342603[more]
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BLAST of DNA methyl-transferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
trdmt17.132e-6840.37tRNA aspartic acid methyltransferase 1 [Source:ZFI... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mapk153.777e-6937.31mitogen-activated protein kinase 15 [Source:Xenbas... [more]
mapk153.196e-6338.25mitogen-activated protein kinase 15 [Source:Xenbas... [more]
mapk152.189e-6136.39mitogen-activated protein kinase 15 [Source:Xenbas... [more]
mapk152.912e-6136.46mitogen-activated protein kinase 15 [Source:Xenbas... [more]
mapk153.373e-5436.65mitogen-activated protein kinase 15 [Source:Xenbas... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Trdmt12.143e-7038.11tRNA aspartic acid methyltransferase 1 [Source:MGI... [more]
Trdmt12.470e-1748.75tRNA aspartic acid methyltransferase 1 [Source:MGI... [more]
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BLAST of DNA methyl-transferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O14717|TRDMT_HUMAN7.005e-7136.68tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo... [more]
sp|O55055|TRDMT_MOUSE1.499e-6938.11tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus ... [more]
sp|Q7YS61|TRDMT_BOVIN3.448e-6937.05tRNA (cytosine-5-)-methyltransferase OS=Bos taurus... [more]
sp|Q54JH6|CMT1_DICDI5.373e-6739.57DNA (cytosine-5)-methyltransferase OS=Dictyosteliu... [more]
sp|Q4G073|TRDMT_RAT3.267e-6636.50tRNA (cytosine(38)-C(5))-methyltransferase OS=Ratt... [more]
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BLAST of DNA methyl-transferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V5UWJ20.000e+099.66DNA methyl-transferase OS=Schmidtea mediterranea O... [more]
A0A4E0QTC64.756e-9243.01tRNA aspartic acid methyltransferase 1 OS=Fasciola... [more]
C4Q8S01.380e-9143.24DNA-methyltransferase 2 isoform 1 OS=Schistosoma m... [more]
A0A504YBD63.797e-9042.90tRNA aspartic acid methyltransferase 1 OS=Fasciola... [more]
A0A4S2LVL83.934e-9043.16Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
trdmt15.110e-7337.70tRNA aspartic acid methyltransferase 1 [Source:NCB... [more]
trdmt17.574e-6336.14tRNA aspartic acid methyltransferase 1 [Source:NCB... [more]
trdmt14.805e-4633.33tRNA aspartic acid methyltransferase 1 [Source:NCB... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA methyl-transferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA methyl-transferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO325541.408e-3142.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA methyl-transferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
trdmt14.637e-7838.70tRNA aspartic acid methyltransferase 1 [Source:NCB... [more]
trdmt12.074e-6537.50tRNA aspartic acid methyltransferase 1 [Source:NCB... [more]
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BLAST of DNA methyl-transferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017337.10.000e+098.34SMESG000017337.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30006325
GenBankAHB82105.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006325 ID=SMED30006325|Name=DNA methyl-transferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1444bp
CACAAATTGTTTAAATAATAAAAATATTTTTTTATAAATGAAGGTTATTG
AATTTTTTTCTGGAATTGGTGGAATTAAATGTGCATTAAATGAGCTAAAT
ATACAAACTGATGTCGTTGCAGCATTTGACATAAATGATTTGGCTAATTC
AGTTTACAGATATAATTTTACTAGTCCATGTTACAATAAAGTTATTGAGT
CTATATCTGCTGAATATTTAATAAGCTTAAATGCAGACATGTGGACTATG
AGTCCTCCATGTCAACCATATACCAGAAATGGAAATATGATGGATCTTGA
TGACCCAAGAACTGTTGCAATGAAACACACACTTTACTTGATATCCCAAG
TCAGGCCTCATTATATTTTCTTTGAAAATGTAAAAGGATTTGAAAGTTCA
AATGGTCAAAAAATGCTTGTTTCTATTCTTTCAGAATCTGCTTATTCCTT
CCAGGAATTTTTATTAAGCCCTTTGCAGTTTGGCGTACCAAATAGTAGAT
TGCGTTACTATCTCATTGCAAAGCTAGAAGGGAAAGGAAGCTTGATGCAA
GATTTGGAAGCGATTTCATATAAGCCTTATTTCGATAGAAAACTCTATCA
ATGTAATTGCCCGGTTTGCTCTGGGAGATCTCGTTCACTGGAAAATGACC
ACGTCAACCATTTTGAGAGAAATCTAGAATTCTGTGATCGAATCTCGGCT
TATTTGGAGGCGGATAATTTAGCTGAGCCTCATGAAGGTCACAAATTGAT
CGATGAGAAAGTATTAGAGAAGGGCTTCAGTAAGCTGGACATTGTCACTG
AGTCGAGCAACAAAACTTGCTGCTTTATCAAGTGTTATGCTAAGAAAATC
GAAGGCTCTGGATCATACTATCAAATGACGTCATGCGATGAAGCTCATCA
ATTGAAGCAATTGCTACTCAATGGTGACATCAGCTCTCTGGACTATGCCA
AACGGCTGAAATTGCGATATTTCAGCCCGAGGGAAATTGCTAATTTCATG
TGTTTTCCTCAATCATTTAGATTCCCAGAAACCGTCACTCGCGCCCAGAG
ATATCGCCTGCTGGGTAACAGCGTGAATGTCAAGGTCGTTGCTCATGTTC
TCCATTGGTTGATTTCAGCGTGATCAATAAATCTTATTCGCCGCTCTTTT
TCATTTGTTGTTTTTATTTTGTTATTTCAAATGTTCTGATCAAGTTACCA
CCTTTGCAACTTCTAAATTGCTGGCAATGGTGATGACAAAATGCAAATGT
AGATTGGCAAAAGAAATCAAATAAATTATTTTCGATTGTTTATTTGCCAG
CAAATATTGAGCCTCGTTGGGAATACTGTAATTGCTCAGATGTACTCTGG
GTTCAACGGGCTGCCGATAGGAGGTCTGTGTTCAGTATTTAGATTTGTTT
CCATCATCTTGTACGTTGTTTCATTATTTGTCTGTTCATTGAAC
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protein sequence of SMED30006325-orf-1

>SMED30006325-orf-1 ID=SMED30006325-orf-1|Name=SMED30006325-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=362bp
MKVIEFFSGIGGIKCALNELNIQTDVVAAFDINDLANSVYRYNFTSPCYN
KVIESISAEYLISLNADMWTMSPPCQPYTRNGNMMDLDDPRTVAMKHTLY
LISQVRPHYIFFENVKGFESSNGQKMLVSILSESAYSFQEFLLSPLQFGV
PNSRLRYYLIAKLEGKGSLMQDLEAISYKPYFDRKLYQCNCPVCSGRSRS
LENDHVNHFERNLEFCDRISAYLEADNLAEPHEGHKLIDEKVLEKGFSKL
DIVTESSNKTCCFIKCYAKKIEGSGSYYQMTSCDEAHQLKQLLLNGDISS
LDYAKRLKLRYFSPREIANFMCFPQSFRFPETVTRAQRYRLLGNSVNVKV
VAHVLHWLISA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000227germ cell
PLANA:0002044germline stem cell
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001525C5_MeTfrase
IPR029063SAM-dependent_MTases
IPR031303C5_meth_CS
Vocabulary: molecular function
TermDefinition
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001525C-5 cytosine methyltransferasePRINTSPR00105C5METTRFRASEcoord: 149..162
score: 39.86
coord: 2..18
score: 37.47
coord: 106..120
score: 42.0
IPR001525C-5 cytosine methyltransferasePFAMPF00145DNA_methylasecoord: 1..356
e-value: 1.9E-39
score: 136.0
IPR001525C-5 cytosine methyltransferaseTIGRFAMTIGR00675TIGR00675coord: 3..355
e-value: 3.2E-38
score: 130.1
IPR001525C-5 cytosine methyltransferasePROSITEPS51679SAM_MT_C5coord: 1..361
score: 39.848
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 3..355
e-value: 1.6E-96
score: 325.3
NoneNo IPR availableGENE3DG3DSA:3.90.120.10coord: 204..346
e-value: 1.6E-96
score: 325.3
NoneNo IPR availablePANTHERPTHR46098FAMILY NOT NAMEDcoord: 1..360
IPR031303DNA methylase, C-5 cytosine-specific, conserved sitePROSITEPS00095C5_MTASE_2coord: 340..358
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 1..358