SMED30006290
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006290 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30006290 aligns in the following genomic locations:
Homology
BLAST of SMED30006290 vs. TrEMBL
Match: A0A564XXJ3 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS496 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 4.168e-20 Identity = 74/258 (28.68%), Postives = 119/258 (46.12%), Query Frame = 1 Query: 298 EKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLP-IQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMS----SNQSNSI------------------------------------DLSYYTL------------INQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYD 912 E C C+RSSP+AS+L +I + G AF G I K +++ + P + Y +Y V +++++ Y++ A+SSG N KR FEG++ GRC I CL+ L W LVSC+ Y ++ + F+ R + +N+++S DLSYY L + + K+ N P IF+ ++ + GSL+ ++ + L C AMN+ RL E RYY+ Sbjct: 13 ESSCTRCLRSSPLASLLLMILVFLGGTAFGVGTIYGRKFFYELLEVDHLFPYMDYTVYAIVGATGLFAIIVYIICAVSSGWNAKRCFEGTRATSCGRCMNATLIVCLISAVLFWTLVSCLLTYPIMSSAFLLYRNDGPARSVTNEASSTVRTARQIEPRTAPSAGLDVDPSEVIASYFTCDPTKADLSYYGLYGTDGKPLMLDTTDLSTKLRN---PLIFICVAFL--GSLITMLAYFLLTCSVAMNYARLKELRYYE 265
BLAST of SMED30006290 vs. TrEMBL
Match: A0A4S2LXA4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005367 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 5.322e-20 Identity = 48/135 (35.56%), Postives = 82/135 (60.74%), Query Frame = 1 Query: 295 AEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLP-IQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNR 696 E C+ C+RSSP+AS+LG I +L G GAF G I + ++ +++ P ++Y++YG V +++ L YL+ A+SSG N K+ FE ++K+ GRC + +I C++ LLW + C+ +Y +I + +R Sbjct: 7 GENSCIRCLRSSPIASLLGFIAVLIGGGAFGGATIFARRFLDDMLSAPHLFPCLEYVIYGLVGATGLFAALAYLVCAISSGRNAKQCFESTRKSACGRCCNMLSIICVVFGVLLWTAIICLMSYSVISFALLMHR 141
BLAST of SMED30006290 vs. TrEMBL
Match: A0A4S2LYM5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005367 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 9.113e-20 Identity = 48/135 (35.56%), Postives = 82/135 (60.74%), Query Frame = 1 Query: 295 AEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLP-IQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNR 696 E C+ C+RSSP+AS+LG I +L G GAF G I + ++ +++ P ++Y++YG V +++ L YL+ A+SSG N K+ FE ++K+ GRC + +I C++ LLW + C+ +Y +I + +R Sbjct: 7 GENSCIRCLRSSPIASLLGFIAVLIGGGAFGGATIFARRFLDDMLSAPHLFPCLEYVIYGLVGATGLFAALAYLVCAISSGRNAKQCFESTRKSACGRCCNMLSIICVVFGVLLWTAIICLMSYSVISFALLMHR 141
BLAST of SMED30006290 vs. TrEMBL
Match: A0A075AJN6 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_00578 PE=4 SV=1) HSP 1 Score: 96.2857 bits (238), Expect = 3.139e-18 Identity = 46/135 (34.07%), Postives = 81/135 (60.00%), Query Frame = 1 Query: 295 AEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLP-IQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNR 696 E C+ C+RSSP+AS+LG I +L G GAF G I + ++ +++ P ++Y++YG + +++ L Y + A+SSG N K+ FE ++K+ GRC + +I C++ LLW + C+ +Y +I + +R Sbjct: 7 GENSCIRCLRSSPIASLLGFIAVLIGGGAFGGATIFARRFLDDMLSAPHLFPCLEYVVYGLMGATGLFAALAYFVCAISSGRNAKQCFESTRKSICGRCCNMLSIICVVFGILLWTAIICLMSYSVISFALLMHR 141
BLAST of SMED30006290 vs. TrEMBL
Match: A0A1S8WJU6 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_09492 PE=4 SV=1) HSP 1 Score: 95.9005 bits (237), Expect = 4.796e-18 Identity = 46/134 (34.33%), Postives = 81/134 (60.45%), Query Frame = 1 Query: 298 EKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLP-IQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNR 696 E C+ C+RSSP+AS+LG I +L G GAF G I + ++ +++ P ++Y++YG + +++ L Y + A+SSG N K+ FE ++K+ GRC + +I C++ LLW + C+ +Y +I + +R Sbjct: 1 ENSCIRCLRSSPIASLLGFIAVLIGGGAFGGATIFARRFLDDMLSAPHLFPCLEYVVYGLMGATGLFAALAYFVCAISSGRNAKQCFESTRKSICGRCCNMLSIICVVFGILLWTAIICLMSYSVISFALLMHR 134
BLAST of SMED30006290 vs. Planmine SMEST
Match: SMESG000072418.1 (SMESG000072418.1) HSP 1 Score: 459.914 bits (1182), Expect = 2.342e-164 Identity = 226/227 (99.56%), Postives = 227/227 (100.00%), Query Frame = 1 Query: 280 MVEIYAEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 960 MVEIYAEKGCVGCMRSSPVASMLGLITILFGIGAFSGGL+IYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF Sbjct: 1 MVEIYAEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLVIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 227
BLAST of SMED30006290 vs. Planmine SMEST
Match: SMESG000072418.1 (SMESG000072418.1) HSP 1 Score: 446.047 bits (1146), Expect = 2.622e-158 Identity = 219/221 (99.10%), Postives = 221/221 (100.00%), Query Frame = 1 Query: 298 EKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 960 +KGCVGCMRSSPVASMLGLITILFGIGAFSGGL+IYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF Sbjct: 47 KKGCVGCMRSSPVASMLGLITILFGIGAFSGGLVIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 267
BLAST of SMED30006290 vs. Planmine SMEST
Match: SMESG000072418.1 (SMESG000072418.1) HSP 1 Score: 433.721 bits (1114), Expect = 3.034e-154 Identity = 213/214 (99.53%), Postives = 214/214 (100.00%), Query Frame = 1 Query: 319 MRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 960 MRSSPVASMLGLITILFGIGAFSGGL+IYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF Sbjct: 1 MRSSPVASMLGLITILFGIGAFSGGLVIYWKSVNKIINYETYLPIQYIMYGTVSIVVVYSMLCYLLAALSSGHNRKRFFEGSKKAKIGRCFTIFNIFCLMITSLLWILVSCVAAYCLIVTCFIHNRINMSSNQSNSIDLSYYTLINQTVKIENDAIPEIFLSISCVTGGSLVIVMGFIHLLCCSAMNFVRLSEERYYDDFSATQIKSEHVLSAF 214 The following BLAST results are available for this feature:
BLAST of SMED30006290 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006290 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006290 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30006290 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006290 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006290 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006290 ID=SMED30006290|Name=SMED30006290|organism=Schmidtea mediterranea sexual|type=transcript|length=1158bpback to top protein sequence of SMED30006290-orf-1 >SMED30006290-orf-1 ID=SMED30006290-orf-1|Name=SMED30006290-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=228bp MVEIYAEKGCVGCMRSSPVASMLGLITILFGIGAFSGGLIIYWKSVNKIIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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