SMED30006247

Overview
NameSMED30006247
Smed IDSMED30006247
Length (bp)2268
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006247 (SMED30006247) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006247 (SMED30006247) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006247 (SMED30006247) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006247 (SMED30006247) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006247

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 21

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30006247SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30006247SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30006247SMESG000017804.1 Contig4736uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30006247SMESG000017804.1 Contig4736newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pigment cellSMED30006247SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SmedASXL_017630SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014562.1 SMESG000014554.1 SMESG000014553.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig33927uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014562.1 SMESG000014554.1 SMESG000014553.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig33927newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000066562.1 SMESG000036854.1 SMESG000014563.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig29967uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000066562.1 SMESG000036854.1 SMESG000014563.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig29967newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig42720uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig42720newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30006247SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30006247SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30006247SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006247SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000017804.1 Contig4736uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006247SMESG000017804.1 Contig4736newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30006247SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30006247SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30006247 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 1072.77 bits (2773), Expect = 0.000e+0
Identity = 537/600 (89.50%), Postives = 558/600 (93.00%), Query Frame = 3 
Query:  108 IQMLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 1895
            ++M +LT+  F L L+ A+++I LFV+SVS FPH IGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHA LNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGS+IYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYN MRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I +NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:    1 MKMQMLTVLLFILHLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 600
[Close]
BLAST of SMED30006247 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 1071.61 bits (2770), Expect = 0.000e+0
Identity = 537/598 (89.80%), Postives = 556/598 (92.98%), Query Frame = 3 
Query:  114 MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 1895
            M +LT+  F L L+ A+++I LFV+SVS FPH IGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHA LNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGS+IYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYN MRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I +NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:    1 MQMLTVLLFILHLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 598
[Close]
BLAST of SMED30006247 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 1070.07 bits (2766), Expect = 0.000e+0
Identity = 536/596 (89.93%), Postives = 555/596 (93.12%), Query Frame = 3 
Query:  120 VLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 1895
            +LT+  F L L+ A+++I LFV+SVS FPH IGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHA LNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGS+IYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYN MRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I +NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:    1 MLTVLLFILHLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 596
[Close]
BLAST of SMED30006247 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 1055.82 bits (2729), Expect = 0.000e+0
Identity = 534/600 (89.00%), Postives = 553/600 (92.17%), Query Frame = 3 
Query:  108 IQMLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 1895
            IQML + L  F L L+ A+++I LFV+SVS FPH IG+AFSLDIQSRSEN KLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHA LNVTEIIYNETANEISMNLR SLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHI PDIKDILQANRAIQCY QDGYPQPSLHFQLIKGPSNASHYYETRKNGS+I+IRSSAPTGSAWMFKCLMKSTIA VAMTKEKNI FRIASHPTSILI PVPDGIMEPNSTIACGPADGYPVARATWEVIAG ESASLYYN MRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I +NPMEICGEKCLNSRI EIEE+V EN RL EDI+ ILN    QM AM K  Q+  +  
Sbjct:    3 IQMLTVLL--FILHLLFAEAEIALFVRSVSNFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEICGEKCLNSRISEIEESVDENERLQEDIRSILNQVKDQMIAMGKGQQKTSRGM 600
[Close]
BLAST of SMED30006247 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 1054.28 bits (2725), Expect = 0.000e+0
Identity = 531/596 (89.09%), Postives = 551/596 (92.45%), Query Frame = 3 
Query:  120 VLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 1895
            +LT+  F L L+ A+++I LFV+SVS FPH IG+AFSLDIQSRSEN KLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHA LNVTEIIYNETANEISMNLR SLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHI PDIKDILQANRAIQCY QDGYPQPSLHFQLIKGPSNASHYYETRKNGS+I+IRSSAPTGSAWMFKCLMKSTIA VAMTKEKNI FRIASHPTSILI PVPDGIMEPNSTIACGPADGYPVARATWEVIAG ESASLYYN MRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I +NPMEICGEKCLNSRI EIEE+V EN RL EDI+ ILN    QM AM K  Q+  +  
Sbjct:    1 MLTVLLFILHLLFAEAEIALFVRSVSNFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEICGEKCLNSRISEIEESVDENERLQEDIRSILNQVKDQMIAMGKGQQKTSRGM 596
The following BLAST results are available for this feature:
BLAST of SMED30006247 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006247 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014544.10.000e+089.50SMESG000014544.1[more]
SMESG000014544.10.000e+089.80SMESG000014544.1[more]
SMESG000014544.10.000e+089.93SMESG000014544.1[more]
SMESG000014542.10.000e+089.00SMESG000014542.1[more]
SMESG000014542.10.000e+089.09SMESG000014542.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006247 ID=SMED30006247|Name=SMED30006247|organism=Schmidtea mediterranea sexual|type=transcript|length=2268bp
CGGAAGTTGAAAATTGAGGTTGATTAACCCTTGTCCTTTGATCAGTTTAT
GTTGGCTTTCAATCATTATAAATTGCGACCTCCTTCATAAACACATTCTA
ATTCAATATTCAAATGCTAGTTCTAACACTTTATTGTTTCTTTTTACCTT
TGATATTGGCGCAATCTCAAATTCCATTGTTTGTCAAATCGGTCTCGAAA
TTTCCACATTTAATTGGTGACGCCTTTTCACTGGATATCCAATCACGTTC
AGAGAATAGCAAACTGAGTTCTGATCCCTCAGATACGATGGTTATACGCA
TTGTGGATCTGAATTTTACGGAGATAGAAAGAGCGTATCTTGTTTACGGG
GCATTATATTTTATGGATGACGAGTTCAAGGGAAATTTAATGGACATCAA
AATATGGGAAAAGAATCAAATGTCCACAAAAATTGATATTTCAATAATAT
TCAAGCGAGAGGCCCATAACAAATATCTAGAAATATTTTACTTTTCTGCT
CCCAATAAATTTGTACGAAGAAAATATTTGAAAAATCTCTCCCTTGCCAA
ACGGTTATTTCATGAACTGATTGACTTGTTTATCCCATCCACATTGGCAC
TCACGCTGCTCCCAATCAAGGTTAACAGAAAGGAAATAATAATAAATGAT
ATGGTAATGGCGTCCTGCAGAGCATTCGGTGGGATACCACAAGAAAAACC
GAGATTCGAGGATCACGCTCACTTGAATGTGACGGAGATAATATATAATG
AAACAGCCAATGAGATCAGTATGAATTTGCGCACATCTCTCGATGACCAT
CTCACTAATTTCAGCTGCAGCGCCGGAAATGTTACGGAAACGTTCATAAT
CCGTATTCATCATCATCCGTCAAAAATTCACATAAAGCCAGATATTAAAG
ATATATTGCAAGCCAACAGAGCCATTCAATGCTATGCCCAAGATGGATAC
CCTCAGCCTTCATTGCATTTCCAGCTCATCAAAGGTCCATCAAACGCATC
TCACTATTATGAGACTAGGAAAAATGGATCCAAAATCTACATCCGTTCAA
GTGCCCCAACCGGATCAGCGTGGATGTTCAAATGCTTAATGAAAAGCACA
ATTGCAGGGGTAGCAATGACCAAGGAAAAGAATATTAACTTTCGAATAGC
CAGCCATCCGACATCAATCCTGATCATGCCAGTGCCAGATGGAATAATGG
AACCGAATTCAACAATTGCTTGTGGCCCAGCTGACGGATATCCTGTTGCC
AGAGCAACATGGGAAGTTATTGCGGGCCCAGAAAGCGCTTCACTATATTA
TAATTTTATGCGCAATGGAACGCAAATTCATCTGAAAGAAAATTGCCCTA
CAAAATCTGAATGGGAAATCAAATGCTCTGTTAAAAGTTGCTTGGAAAAT
GTTTGTGTAATCCTCAATCGGACAATTTCATTTAGAATAGCAAATCACCC
GACCGCATTATATATTCAACCAATCATCAAAAAGAGATTCCGATCAAACT
CAACAATAAAATGCTTCACAAAAGATGGATATCCAGATCCAGAAATAAAT
TGGCAATTAATTGAAGGCCCCCCAAATGCTAACTTGTTTTACAATATTCC
TCGAAAGGGTGACCAAATCTACATCAATAGCAACAGCCAAACCGGTTCAA
AATGGACGTTCAGATGCACAATGAAAAGCTTTCTCGTGAATACCAATAAA
ATTTTGAATAAAACTATTTCATTTGCTATTACTAGGAATCCGATGGAAAT
TTGTGGGGAGAAATGTTTAAATTCACGGATTTTTGAAATTGAAGAAGCGG
TAAAGGAGAACGTGAGGCTAAATGAGGATATCAAGTTGATATTGAATCAA
ATGGAAGCCATGAGAAAGGAACACCAAGAAATAAGAAAGGCGTTTCTATA
TTTTCAATGGAATTTTAATACCACAACTTATAATATACAAAAAACCCAAA
CTTTTACTTCAGCGTTCCCATTGTCTGTTCGATTCCAAACGGAATTCGTG
GAAAAGGACCACAAACTATTCAAATGCGGATTGATAGTTAGGAATGACAA
TCCACAAATGTCTGTAAACTTGATAGTGACATACGAATGTTGGATATCTG
CAAAATCGTCGCCCCTCAAATTGATATTTAATGTTAAAGTCAAACCTCTA
GAAGAAAATAAAGAAGAAAAAAATTTCTTCATATTTCCTTCTAGTAAGAT
ATTTGGCCAAAACCTTTATCAAACGAAACTCTCTGTTGGATGTCATTTCA
TTAAATTTCAATAATAAA
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protein sequence of SMED30006247-orf-1

>SMED30006247-orf-1 ID=SMED30006247-orf-1|Name=SMED30006247-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=717bp
MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRSENSK
LSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEK
NQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFH
ELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFED
HAHLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIIRIHH
HPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYE
TRKNGSKIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPT
SILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNFMR
NGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALY
IQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPRKGD
QIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEK
CLNSRIFEIEEAVKENVRLNEDIKLILNQMEAMRKEHQEIRKAFLYFQWN
FNTTTYNIQKTQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVRNDNPQMS
VNLIVTYECWISAKSSPLKLIFNVKVKPLEENKEEKNFFIFPSSKIFGQN
LYQTKLSVGCHFIKFQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding