GTP-binding protein GEM
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006233 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of GTP-binding protein GEM vs. Ensembl Human
Match: RIT1 (Ras like without CAAX 1 [Source:HGNC Symbol;Acc:HGNC:10023]) HSP 1 Score: 87.0409 bits (214), Expect = 6.815e-19 Identity = 59/216 (27.31%), Postives = 107/216 (49.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R+++ E+G LA+++ C F E S A + +DD+ ++ +I +++++E V A +K NS+ + F K S
Sbjct: 19 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREI-------RRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSV 218
BLAST of GTP-binding protein GEM vs. Ensembl Human
Match: RIT1 (Ras like without CAAX 1 [Source:HGNC Symbol;Acc:HGNC:10023]) HSP 1 Score: 87.4261 bits (215), Expect = 7.492e-19 Identity = 59/216 (27.31%), Postives = 107/216 (49.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R+++ E+G LA+++ C F E S A + +DD+ ++ +I +++++E V A +K NS+ + F K S
Sbjct: 36 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREI-------RRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSV 235
BLAST of GTP-binding protein GEM vs. Ensembl Human
Match: RIT1 (Ras like without CAAX 1 [Source:HGNC Symbol;Acc:HGNC:10023]) HSP 1 Score: 83.9593 bits (206), Expect = 8.011e-18 Identity = 59/217 (27.19%), Postives = 108/217 (49.77%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDD-DPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R+++ E+G LA+++ C F E S A + +DD+ ++ +I +++++E V A +K NS+ + F K S
Sbjct: 19 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREI-------RRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSV 219
BLAST of GTP-binding protein GEM vs. Ensembl Human
Match: RIT2 (Ras like without CAAX 2 [Source:HGNC Symbol;Acc:HGNC:10017]) HSP 1 Score: 82.0333 bits (201), Expect = 3.654e-17 Identity = 61/189 (32.28%), Postives = 100/189 (52.91%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA---KIILNYIR--CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKY-----KKEKRED 1389
S + + ++GA VGKS+M QF++ ++ + P + +T + +DN L LDT + Q +G++I Y++ + SF+ A K ++ +R EIP L LV NK DLE+ R++S+E+G LA++Y+C F E S AL +DD ++ +I +KE KK KR+D
Sbjct: 18 SREYKVVMLGAGGVGKSAMTMQFISHQF-PDYHDPTIEDAYKT-QVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIP----LVLVGNKIDLEQFRQVSTEEGLSLAQEYNCGFFETSAALRFCIDDAFHGLVREI-RKKESMPSLMEKKLKRKD 199
BLAST of GTP-binding protein GEM vs. Ensembl Human
Match: RRAD (RRAD, Ras related glycolysis inhibitor and calcium channel regulator [Source:HGNC Symbol;Acc:HGNC:10446]) HSP 1 Score: 83.1889 bits (204), Expect = 6.072e-17 Identity = 51/163 (31.29%), Postives = 82/163 (50.31%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ + ++GAP VGKS++ F E + E A + +I +D E +L D + D Y+IVY++ + SFE A ++ + R D + LV NKSDL R RE+S ++G+ A +DC+F+E S AL+H V L ++ +I
Sbjct: 92 YKVLLLGAPGVGKSALARIFGGVE---DGPEAEAAGHTYDRSIVVDGEEASLMVYDIWEQDGGRWLPGHCMAMGDAYVIVYSVTDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQI 251
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Match: Y52B11A.4 (pep chromosome:WBcel235:I:10979802:11004283:-1 gene:WBGene00013124.1 transcript:Y52B11A.4.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y52B11A.4) HSP 1 Score: 88.1965 bits (217), Expect = 1.821e-18 Identity = 102/366 (27.87%), Postives = 156/366 (42.62%), Query Frame = 1
Query: 274 LSMDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLPSDFESSSREELPCENRRDSLQPSSFRNRSIKENRSHSSLRQTPEIFE---IADEGSQNLYRVRSLKKTPKG-VISKGDSVRNHSNQSLEKE---FVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVA-NKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
L + + +ARS S K+ PKP+ R+ + P + R P +RR SLQ S I E R+ P E + S++ RVR K KG V+S+GDS R + + + +P + S SR S S E + ++ M N S + ++ I VVG GKSS+ Q +TSEY N+ E + TV+I + E +L F E D + + + +++VY+ID S++ A + L IR + LVA NK DLER R ++ ++ + E EIS AL+H VDDL+V ++ +I
Sbjct: 68 LGVTDMRNSTTVARSQSFKH------RPKPMVEVRRMAIDDVGPHE----RRRSTPTVSRRPSLQKSR-----IMEPRTDVWPEAKPIGLEERFLKLPDSEDYTRVRQFKIDEKGAVVSRGDSFRRKRTPTYKSDKSPSPFPVSVSTDSARGSRSESEAS-EPLISDDVAKMTVNEMTNAS-----------------TSHKTYKIYVVGDTGTGKSSLISQLITSEY-KNAFADEIQDYENTVSICIGGVECDLVFF-----ESDMSDPCWLTNEIHAFLVVYSIDSKSSWKQAMVALEMIRDRPGTRNLPTLVAGNKIDLERKRTVTKQEVRAAKAAMGFEHFEISVALDHDVDDLLVGLVAEI 394
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Match: Y52B11A.4 (pep chromosome:WBcel235:I:10979607:11004283:-1 gene:WBGene00013124.1 transcript:Y52B11A.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y52B11A.4) HSP 1 Score: 88.1965 bits (217), Expect = 1.821e-18 Identity = 102/366 (27.87%), Postives = 156/366 (42.62%), Query Frame = 1
Query: 274 LSMDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLPSDFESSSREELPCENRRDSLQPSSFRNRSIKENRSHSSLRQTPEIFE---IADEGSQNLYRVRSLKKTPKG-VISKGDSVRNHSNQSLEKE---FVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVA-NKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
L + + +ARS S K+ PKP+ R+ + P + R P +RR SLQ S I E R+ P E + S++ RVR K KG V+S+GDS R + + + +P + S SR S S E + ++ M N S + ++ I VVG GKSS+ Q +TSEY N+ E + TV+I + E +L F E D + + + +++VY+ID S++ A + L IR + LVA NK DLER R ++ ++ + E EIS AL+H VDDL+V ++ +I
Sbjct: 68 LGVTDMRNSTTVARSQSFKH------RPKPMVEVRRMAIDDVGPHE----RRRSTPTVSRRPSLQKSR-----IMEPRTDVWPEAKPIGLEERFLKLPDSEDYTRVRQFKIDEKGAVVSRGDSFRRKRTPTYKSDKSPSPFPVSVSTDSARGSRSESEAS-EPLISDDVAKMTVNEMTNAS-----------------TSHKTYKIYVVGDTGTGKSSLISQLITSEY-KNAFADEIQDYENTVSICIGGVECDLVFF-----ESDMSDPCWLTNEIHAFLVVYSIDSKSSWKQAMVALEMIRDRPGTRNLPTLVAGNKIDLERKRTVTKQEVRAAKAAMGFEHFEISVALDHDVDDLLVGLVAEI 394
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Match: rheb-1 (GTP-binding protein Rheb homolog 1 [Source:UniProtKB/Swiss-Prot;Acc:P34443]) HSP 1 Score: 69.707 bits (169), Expect = 1.690e-13 Identity = 51/182 (28.02%), Postives = 96/182 (52.75%), Query Frame = 1
Query: 835 ANLKSRSTSFN--IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEK----ANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFE-----YAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
++L+S S N + V+G P VGKS++ +F + + PE+ + + I +R+++L DT ++ + +G+++VYAID+ SFE Y KI+ Y IP + +V NK+DL R + +E+G+ LA+Q+D +F+EI+ ++ V ++ ++ +I
Sbjct: 3 SSLQSNRQSLNRKVAVMGYPHVGKSALVLRFTQNIF------PERYESTIEDQHSKHIAAFHRDYHLRVTDTAGQQEYTVFPRSCSLDINGFILVYAIDDRKSFEMCSNIYEKIVRTYGDTSIP----IVIVGNKTDLSTQRVVRAEEGEELARQWDAKFVEITARESNRVHEVFELLLREI 174
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Match: ral-1 (RAL (Ras-related GTPase) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N3F8]) HSP 1 Score: 67.3958 bits (163), Expect = 1.352e-12 Identity = 50/188 (26.60%), Postives = 89/188 (47.34%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSA--LFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTN 1425
+ +VG VGKS++ QF+ E++ P KA+ R + LD E ++ LDT ED Y + +G++ V++I + +SFE I DS+ + LV NK D+ R +S+E + A+Q+ C ++E S VD + ++ ++ +K + + D +A K +
Sbjct: 20 VIMVGTGGVGKSALTLQFMYDEFVEEYE-PTKADSYRKKVV-LDGEECSIDILDTAGQEDYSAIRDNYYRSGEGFICVFSILDMESFEATNEFREQILRVKNSDSSVPIVLVGNKGDMRDQRVVSAELCRQRAEQWGCHYVETSAKRRENVDKVFYDLMREMKRRKGGSQAQTGIDASASSGRKKRSG 205
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Match: ral-1 (RAL (Ras-related GTPase) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N3F8]) HSP 1 Score: 67.781 bits (164), Expect = 1.762e-12 Identity = 57/234 (24.36%), Postives = 105/234 (44.87%), Query Frame = 1
Query: 760 LRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFN----------IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSA--LFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTN 1425
++LK + + F R D+ + + + S+ S + +VG VGKS++ QF+ E++ P KA+ R + LD E ++ LDT ED Y + +G++ V++I + +SFE I DS+ + LV NK D+ R +S+E + A+Q+ C ++E S VD + ++ ++ +K + + D +A K +
Sbjct: 15 IKLKFLALWHFLFRRKSDEPEFYDPEAMASKKASGTLPPQQQVVHKVIMVGTGGVGKSALTLQFMYDEFVEEYE-PTKADSYRKKVV-LDGEECSIDILDTAGQEDYSAIRDNYYRSGEGFICVFSILDMESFEATNEFREQILRVKNSDSSVPIVLVGNKGDMRDQRVVSAELCRQRAEQWGCHYVETSAKRRENVDKVFYDLMREMKRRKGGSQAQTGIDASASSGRKKRSG 246
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Match: Rgk1 (gene:FBgn0264753 transcript:FBtr0334282) HSP 1 Score: 118.627 bits (296), Expect = 3.028e-28 Identity = 85/305 (27.87%), Postives = 153/305 (50.16%), Query Frame = 1
Query: 469 PSSFRNRSIKENRSHSSLR--------QTPEIFEIADEG-SQNLYRVRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLG--NSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRC-EIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
P R++S+K S +S R Q + + + G + YR+R T KGV+++GDS+++ ++S + H + ++++P S + + R T+ P + + + ++G P VGKSS+ QF+TSEYL ++SI +++ E + V++ L E L F+D E P+ ++ GY ++Y+ + SF A+ +L + + A+ LV+NK+DL R R ++SE+GK +A YDC+F+E S +NH VD+L+V ++ +I
Sbjct: 122 PHHSRSQSVKMPGSRASGRPNQLCLPQQRSRVASMPNTGVEEEYYRLRHFSITGKGVVNRGDSLKSRRSRSNNSVASSNSSTEHLT-TQQQLSAPASVSARTSLASSRESSTSNPG---------------------NGPYRVLMLGGPAVGKSSLVSQFMTSEYLHAYDTSIDDESGE-KAVSVLLSGEESELIFIDHGYTEMT-PDECLTNYDPHGYCVIYSAADRSSFSVAEQVLQVLWTNQNIAQKAVILVSNKADLARSRLVTSEEGKAMATAYDCKFIETSVGINHNVDELLVGLLSQI 402
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Match: Rgk1 (gene:FBgn0264753 transcript:FBtr0334283) HSP 1 Score: 118.627 bits (296), Expect = 1.362e-27 Identity = 76/261 (29.12%), Postives = 136/261 (52.11%), Query Frame = 1
Query: 574 YRVRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLG--NSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRC-EIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
YR+R T KGV+++GDS+++ ++S + H + ++++P S + + R T+ P + + + ++G P VGKSS+ QF+TSEYL ++SI +++ E + V++ L E L F+D E P+ ++ GY ++Y+ + SF A+ +L + + A+ LV+NK+DL R R ++SE+GK +A YDC+F+E S +NH VD+L+V ++ +I
Sbjct: 1047 YRLRHFSITGKGVVNRGDSLKSRRSRSNNSVASSNSSTEHLTTQQ-QLSAPASVSARTSLASSRESSTSNPG---------------------NGPYRVLMLGGPAVGKSSLVSQFMTSEYLHAYDTSIDDESGE-KAVSVLLSGEESELIFIDHGYTEMT-PDECLTNYDPHGYCVIYSAADRSSFSVAEQVLQVLWTNQNIAQKAVILVSNKADLARSRLVTSEEGKAMATAYDCKFIETSVGINHNVDELLVGLLSQI 1283
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Match: Rgk1 (gene:FBgn0264753 transcript:FBtr0334284) HSP 1 Score: 113.62 bits (283), Expect = 1.675e-26 Identity = 79/276 (28.62%), Postives = 135/276 (48.91%), Query Frame = 1
Query: 574 YRVRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYL---------GNSSI---PEKANE-----SRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRC-EIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
YR+R T KGV+++GDS+++ ++S + H + ++++P S + + R T+ P + + + ++G P VGKSS+ QF+TSEYL GN I EKA + + V++ L E L F+D E P+ ++ GY ++Y+ + SF A+ +L + + A+ LV+NK+DL R R ++SE+GK +A YDC+F+E S +NH VD+L+V ++ +I
Sbjct: 166 YRLRHFSITGKGVVNRGDSLKSRRSRSNNSVASSNSSTEHLT-TQQQLSAPASVSARTSLASSRESSTSNPG---------------------NGPYRVLMLGGPAVGKSSLVSQFMTSEYLHAYDTSIDLGNCDIRILIEKAADDDESGEKAVSVLLSGEESELIFIDHGYTEMT-PDECLTNYDPHGYCVIYSAADRSSFSVAEQVLQVLWTNQNIAQKAVILVSNKADLARSRLVTSEEGKAMATAYDCKFIETSVGINHNVDELLVGLLSQI 418
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Match: Rgk2 (gene:FBgn0085419 transcript:FBtr0112633) HSP 1 Score: 109.383 bits (272), Expect = 7.707e-25 Identity = 88/295 (29.83%), Postives = 153/295 (51.86%), Query Frame = 1
Query: 559 GSQNLYRVRSLKKTPKGVISKGDSV---RNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNV-SFERSRDDSQIHWHANLKSRSTS-FNIQVVGAPLVGKSSMCEQFLTSEYLG--NSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCE-IPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSH 1419
S +LYR+R + V++ GDS+ R+ SN S VNS S +++ NV S + DDS+ NL + + + ++G VGK+++ QF+TSEY+ ++S+ ++ E +TV++ LD+ E ++ F+D E N++ Y G ++V+++ + SF A+ I+NY+ E D A+ LV NK+DL R R I+S++GK LA D +F+E S+ + H VD+L+V I+ ++ + K K+EK+ + K ++H
Sbjct: 446 ASDDLYRLRHFSISKGNVVNCGDSIISRRSRSNTS--------------------VNSTNSRASERSPFEGSCCGAGYANVDSLPATPDDSE-----NLDPPPPARYRVVMLGDAGVGKTALVNQFMTSEYMHTYDASLDDEFGE-KTVSVLLDDEESDMVFIDHPSVEMSVENSLSTYE-PHGCVVVFSVVDRSSFRVAEEIINYLWQENYTKDKAVILVGNKADLARARLITSQEGKALAASRDAKFIETSSGIQHNVDELLVGILKQM---RLKEKREKKATASKMKTSRTH 710
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Match: Rgk2 (gene:FBgn0085419 transcript:FBtr0340386) HSP 1 Score: 107.071 bits (266), Expect = 4.249e-24 Identity = 87/296 (29.39%), Postives = 149/296 (50.34%), Query Frame = 1
Query: 559 GSQNLYRVRSLKKTPKGVISKGDSV---RNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNV-SFERSRDDSQIHWHANLKSRSTS-FNIQVVGAPLVGKSSMCEQFLTSEYLGN---SSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCE-IPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSH 1419
S +LYR+R + V++ GDS+ R+ SN S VNS S +++ NV S + DDS+ NL + + + ++G VGK+++ QF+TSEY+ S + + +TV++ LD+ E ++ F+D E N++ Y G ++V+++ + SF A+ I+NY+ E D A+ LV NK+DL R R I+S++GK LA D +F+E S+ + H VD+L+V I+ ++ + K K+EK+ + K ++H
Sbjct: 446 ASDDLYRLRHFSISKGNVVNCGDSIISRRSRSNTS--------------------VNSTNSRASERSPFEGSCCGAGYANVDSLPATPDDSE-----NLDPPPPARYRVVMLGDAGVGKTALVNQFMTSEYMHTYDASLVLDDEFGEKTVSVLLDDEESDMVFIDHPSVEMSVENSLSTY-EPHGCVVVFSVVDRSSFRVAEEIINYLWQENYTKDKAVILVGNKADLARARLITSQEGKALAASRDAKFIETSSGIQHNVDELLVGILKQM---RLKEKREKKATASKMKTSRTH 712
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Match: rergla (RERG/RAS-like a [Source:NCBI gene;Acc:436767]) HSP 1 Score: 82.8037 bits (203), Expect = 8.680e-18 Identity = 55/157 (35.03%), Postives = 84/157 (53.50%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTV---TIDLDNREWNLTFLDTVRDEDDDPNNI--QIYHNADGYMIVYAIDEYDSFEYAKIILNYIR------CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTA 1299
+ V+G+ VGKS++ +FLT ++G E A+ S + + +D R+ NL D D + + QI H ADG+++VY I + SF AK I++ IR + DS +FLV NK DL RE+ E+G+ LA + C+F E+S A
Sbjct: 4 IKVAVLGSEGVGKSALIVRFLTRRFIG-----EYASSSECIYRKRMSIDARQLNLELHDPCSQPCDGKSTLNEQI-HWADGFVVVYDISDRSSFLTAKAIVHLIRELHLGATKRDSDSVIFLVGNKQDLCHMREVDREEGQKLASEGRCQFYELSAA 154
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Match: rit1 (Ras-like without CAAX 1 [Source:ZFIN;Acc:ZDB-GENE-070912-329]) HSP 1 Score: 82.0333 bits (201), Expect = 1.835e-17 Identity = 52/184 (28.26%), Postives = 93/184 (50.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKAN----ESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI-CHQKEKYKKEKRE 1386
S + + ++G VGKS++ QF++ + PE + ++ I +D+ NL LDT + Q +G++I Y+I + SF+ A+ I R D+ + LV NKSDL R++S E+GK LA+++ C F E S A + +D++ ++ +I H+ E + +R+
Sbjct: 11 SREYKLVMLGEGGVGKSAIIMQFISHRF------PEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKQLIYRVRRTVDTPVVLVGNKSDLVHLRQVSVEEGKQLAREFQCPFFETSAAFRYYIDEVFAALVRQIRQHEAEMVRDSERK 188
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Match: rerglb (RERG/RAS-like b [Source:ZFIN;Acc:ZDB-GENE-040724-73]) HSP 1 Score: 77.411 bits (189), Expect = 6.820e-16 Identity = 61/204 (29.90%), Postives = 102/204 (50.00%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQ-IYHNADGYMIVYAIDEYDSFEYAKIILNYIR------C--EIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHM-VDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRK 1449
+ ++G+ GKS++ +FLT ++G + AN + +D R+ NL D + ++ ADG+++VY I + SF AK IL I+ C E+P + LV NK DL R++S E+G+ L+++Y C F E+S A N++ + +L K+I + Q K++ ++R KS I N F K
Sbjct: 6 LALLGSEGAGKSAVLVRFLTKRFIGEYA--SNANSLYHKRLSIDGRQLNLEVFDPCSQSGESRCILEEPVDWADGFVVVYTISDRTSFLNAKNILAQIKESRRETCKGEVP----VCLVGNKQDLCHSRQVSEEEGRVLSQEYKCLFQEVSAAENYLEISNLFTKLIRHVMEQL-KHRGDRRR----YSGSKSMAKLINNVFGK 198
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Match: gem (GTP binding protein overexpressed in skeletal muscle [Source:ZFIN;Acc:ZDB-GENE-060825-251]) HSP 1 Score: 78.9518 bits (193), Expect = 9.033e-16 Identity = 53/164 (32.32%), Postives = 80/164 (48.78%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQ-IYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPD-SALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
F + ++G VGKS++ F + S E TI +D ++T LDT +D+ Q D ++IVYAI + SF A + +R E D + + LV NK DL R RE+S +G+ A +DC+F+E S A+ H V L II ++
Sbjct: 71 FTVVLLGDNGVGKSALASIFAGASDSMGSECELYGGEIFEQTITVDGERASVTLLDTWDSQDEGSWTQQRCLQTGDAFIIVYAITDRSSFLRASDLRVQLRREREVDRTPIILVGNKCDLVRCREVSISEGRSSAAVFDCKFIETSAAMQHNVWPLFEGIIRQL 234
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Match: rerglb (RERG/RAS-like b [Source:ZFIN;Acc:ZDB-GENE-040724-73]) HSP 1 Score: 76.2554 bits (186), Expect = 1.522e-15 Identity = 61/204 (29.90%), Postives = 101/204 (49.51%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQ-IYHNADGYMIVYAIDEYDSFEYAKIILNYIR------C--EIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHM-VDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRK 1449
+ ++G+ GKS++ +FLT ++G + AN + +D R+ NL D + ++ ADG+++VY I + SF AK IL I+ C E+P + LV NK DL R++S E+G L+++Y C F E+S A N++ + +L K+I + Q K++ ++R KS I N F K
Sbjct: 6 LALLGSEGAGKSAVLVRFLTKRFIGEYA--SNANSLYHKRLSIDGRQLNLEVFDPCSQSGESRCILEEPVDWADGFVVVYTISDRTSFLNAKNILAQIKESRRETCKGEVP----VCLVGNKQDLCHSRQVSEEEGSVLSQEYKCLFQEVSAAENYLEISNLFTKLIRHVMEQL-KHRGDRRR----YSGSKSMAKLINNVFGK 198
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Match: mark4 (MAP/microtubule affinity-regulating kinase 4 [Source:Xenbase;Acc:XB-GENE-6042140]) HSP 1 Score: 87.4261 bits (215), Expect = 1.907e-18 Identity = 58/177 (32.77%), Postives = 93/177 (52.54%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKRED 1389
+ + ++G VGKSS+ F E L + E+A + +I +D E L D +D+ + Q D Y+IVY++ + SFE A ++ + R D + LV NKSDL R RE+S E+G+ A +DC+F+E S +L+H V DL I+ +I + +K+ +ED
Sbjct: 87 YKVILLGEHGVGKSSLARIFGGVEDLHD---VEEAGNTYDRSIVVDGEEACLLVFDIWEQDDNHWLHNQCMKMGDAYVIVYSVTDKASFEKASELRIQLRRARQSEDIPIILVGNKSDLVRSREVSVEEGRACAVVFDCKFIETSASLHHNVKDLFEGIVRQI-----RLRKDSKED 255
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Match: rem1 (RAS (RAD and GEM)-like GTP-binding 1 [Source:NCBI gene;Acc:100124825]) HSP 1 Score: 83.9593 bits (206), Expect = 2.096e-17 Identity = 62/222 (27.93%), Postives = 112/222 (50.45%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHN----ADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQSTIDNH*NDFISCH 1512
+ ++G P VGKSS+ F + + E+ E T+ +D +E L +DT DE + I+ + + Y+IVY++ + SFE A ++ + R + + LV NK+DL R RE+S E+G+ A +DC+F+E S L H V +L ++ +I ++E + + +++K +S T F + +++S+S++ H SCH
Sbjct: 62 LRVVLLGDPGVGKSSLVAIFRREQ---DREAAEQQLEVFDCTLMVDGKETTLLVMDT--DERNMKKAIESSDSPMRLGNAYIIVYSVTDRASFESASELRIQLRRTRQAENIPIILVGNKTDLVRSREVSVEEGRACAVVFDCKFIETSAVLQHNVQELFEGMVRQIRLRREGADTAEGFRMQSQRK-ESLTKRARRFLDRLVTRNSRSSLKVHSR---SCH 274
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Match: plekhf2 (pleckstrin homology and FYVE domain containing 2 [Source:Xenbase;Acc:XB-GENE-959911]) HSP 1 Score: 77.7962 bits (190), Expect = 2.949e-15 Identity = 59/208 (28.37%), Postives = 105/208 (50.48%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQF--LTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNI--QIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSK 1461
ST + + ++G VGKS++ F + G+ + + R + +D ++ L +D ++ D N I Q D Y+IVY+I + SFE A ++ + R D + LV NKSDL R RE+S +G+ A +DC+F+E S A+ H V +L I+ ++ +++ +K +R + +K+ +S F+ K +K
Sbjct: 76 STYYRVVLIGEQGVGKSTLANIFAGVPDSVDGDCDMLAENTYERNLMVDGESA--TLILIDMWENKAQD-NWIREQCMQVGDAYLIVYSITDRASFEKASELRIQLRRARQTEDIPIILVGNKSDLVRCREVSVSEGRACAVVFDCKFIETSAAVQHNVKELFEGIVRQVRLRRDSKEKNERR-LAYQKRRESIPKKARRFWGKIVAK 279
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Match: ENSXETT00000044277.1 (ras-like protein family member 11A-like [Source:NCBI gene;Acc:100488596]) HSP 1 Score: 73.1738 bits (178), Expect = 4.833e-14 Identity = 53/154 (34.42%), Postives = 84/154 (54.55%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDT--VRDEDDDPN-------NIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDS--ALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEIST 1296
I V+G VGK+++ +FLT ++G+ A SR VTI D + +L DT V EDD + N IY ADGY+ V++I +Y+SF + + ++R +I P++ L LV NK+DL R R++ ++G LA + + E+S
Sbjct: 29 IVVLGGSGVGKTALVVRFLTKRFIGDYEANAGALYSRKVTI--DGEQISLQVQDTPYVSMEDDTDSISCQEQINRSIYW-ADGYVFVFSITDYNSFRVIRPLYQHLR-KIHPNTKIPLLLVGNKADLLRARQVDPDEGLLLANEVGGFYAEVSA 178
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Match: rheb (Ras homolog, mTORC1 binding [Source:NCBI gene;Acc:548676]) HSP 1 Score: 70.8626 bits (172), Expect = 1.039e-13 Identity = 54/163 (33.13%), Postives = 89/163 (54.60%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYH-NADGYMIVYAIDEYDSFEYAKII----LNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKII 1338
I ++G VGKSS+ QF+ +++ +S P N + T I ++ +E+ L +DT +D+ Q Y + +GY++VY++ SFE K+I L+ + + ++P + LV NK DL R IS E+GK LA+ ++ FLE S N D+ +II
Sbjct: 9 IAILGYRSVGKSSLTIQFVEGQFV-DSYDPTIEN-TFTKQISVNGQEYQLQLVDTA-GQDEFSIIPQTYSIDINGYILVYSVTSIKSFEVIKVIHEKLLDMVGKVQVP----IMLVGNKKDLHMERVISYEEGKSLAESWNAAFLESSAKENQTAVDVFKRII 164
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Match: Rit1 (Ras-like without CAAX 1 [Source:MGI Symbol;Acc:MGI:108053]) HSP 1 Score: 87.8113 bits (216), Expect = 2.923e-19 Identity = 60/216 (27.78%), Postives = 107/216 (49.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R++S E+G LA+++ C F E S A + +DD+ ++ +I +K+++E V A +K NS+ + F + S
Sbjct: 19 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVSKEEGLSLAREFSCPFFETSAAYRYYIDDVFHALVREI-------RKKEKELVLAMEKKAKPKNSVWKRLKSPFRRKKDSV 218
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Match: Rrad (Ras-related associated with diabetes [Source:MGI Symbol;Acc:MGI:1930943]) HSP 1 Score: 83.9593 bits (206), Expect = 2.372e-17 Identity = 54/177 (30.51%), Postives = 89/177 (50.28%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKRED 1389
+ + ++GAP VGKS++ F E + E A + +I +D E +L D + D Y+IVY+I + SFE A ++ + R D + LV NKSDL R RE+S ++G+ A +DC+F+E S AL+H V L ++ +I + +++ +ED
Sbjct: 91 YKVLLLGAPGVGKSALARIFGGIE---DGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQI-----RLRRDSKED 259
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Match: Rasl11a (RAS-like, family 11, member A [Source:MGI Symbol;Acc:MGI:1916145]) HSP 1 Score: 81.2629 bits (199), Expect = 6.305e-17 Identity = 58/224 (25.89%), Postives = 108/224 (48.21%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQI--------------YHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPD--SALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNF---FRKHFSKHSQST 1476
+ V+GA VGKS+M +FLT ++G+ SR V ++ D + D P IQ H A+G+++VY+I +Y+S++ + + +IR ++ PD + +F+V NK DL R++ + +G LA + FLEIST+ N+ +D + + +C + K + E A P+ + ++ + FR+ S ++++
Sbjct: 28 IKLAVLGAGCVGKSAMIVRFLTKRFIGDYEPNTGKLYSRLVYVEGDQLSLQI---------QDTPGGIQAQDSLSQMVDSLTKSVHWAEGFLLVYSITDYESYQSIRPLYQHIR-KVHPDGKAPIFIVGNKGDLLHARQVQTHEGLQLANELGSLFLEISTSENY--ED-VCDVFQHLCKEVIKVHRLGGERRRASVIPRPRSPNMQDLKRRFRQALSSKAKAS 238
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Match: Rit2 (Ras-like without CAAX 2 [Source:MGI Symbol;Acc:MGI:108054]) HSP 1 Score: 79.337 bits (194), Expect = 1.829e-16 Identity = 53/170 (31.18%), Postives = 91/170 (53.53%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA---KIILNYIR--CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
S + + ++GA VGKS++ QF++ ++ + P + +T + +DN L LDT + Q +G++I Y++ + SF+ A K ++ +R EIP L LV NK DLE+ R++S+E+G +LA+ Y+C F E S AL +DD ++ +I
Sbjct: 18 SREYKVVMLGAGGVGKSAVTMQFISHQF-PDYHDPTIEDAYKT-QVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIP----LVLVGNKIDLEQFRQVSTEEGMNLARDYNCAFFETSAALRFGIDDAFQGLVREI 181
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Match: Rem1 (rad and gem related GTP binding protein 1 [Source:MGI Symbol;Acc:MGI:1097696]) HSP 1 Score: 78.5666 bits (192), Expect = 1.224e-15 Identity = 55/192 (28.65%), Postives = 91/192 (47.40%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTS------EYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQ--IYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKP 1410
+ + ++G P VGK+S+ F E LG + E+ T+ +D + L +DT E D + Q Y+IVY+I + SFE A ++ + R + LV NK+DL R RE+S E+G+ A +DC+F+E S L H V +L ++ ++ + +R+D A + P
Sbjct: 81 YRVVLLGDPGVGKTSLASLFAEKQDRDPHEQLGG--VYER-------TLSVDGEDTTLVVMDTWEAEKLDESWCQESCLQAGSAYVIVYSIADRSSFESASELRIQLRRTHQANHVPIILVGNKADLARCREVSVEEGRACAVVFDCKFIETSATLQHNVTELFEGVVRQL--------RLRRQDNAAPETP 255
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Match: sp|P70426|RIT1_MOUSE (GTP-binding protein Rit1 OS=Mus musculus OX=10090 GN=Rit1 PE=1 SV=1) HSP 1 Score: 87.8113 bits (216), Expect = 2.044e-18 Identity = 60/216 (27.78%), Postives = 107/216 (49.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R++S E+G LA+++ C F E S A + +DD+ ++ +I +K+++E V A +K NS+ + F + S
Sbjct: 19 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVSKEEGLSLAREFSCPFFETSAAYRYYIDDVFHALVREI-------RKKEKELVLAMEKKAKPKNSVWKRLKSPFRRKKDSV 218
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Match: sp|Q92963|RIT1_HUMAN (GTP-binding protein Rit1 OS=Homo sapiens OX=9606 GN=RIT1 PE=1 SV=1) HSP 1 Score: 87.0409 bits (214), Expect = 3.273e-18 Identity = 59/216 (27.31%), Postives = 107/216 (49.54%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQST 1476
S + + ++GA VGKS+M QF++ + PE ++ + I +D+ NL LDT + Q +G++I Y+I + SF E+ ++I R + D+ + LV NKSDL++ R+++ E+G LA+++ C F E S A + +DD+ ++ +I +++++E V A +K NS+ + F K S
Sbjct: 19 SREYKLVMLGAGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTD---DTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREI-------RRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSV 218
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Match: sp|P55043|RAD_RAT (GTP-binding protein RAD OS=Rattus norvegicus OX=10116 GN=Rrad PE=2 SV=2) HSP 1 Score: 88.9669 bits (219), Expect = 3.719e-18 Identity = 54/176 (30.68%), Postives = 87/176 (49.43%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKRED 1389
+ + GAP VGKS++ F E + E A + +I +D E +L D + D Y+IVY+I + SFE A + +R D + LV NKSDL R RE+S ++G+ A+ +DC+F+E S AL+H V L ++ +I + +++ +ED
Sbjct: 91 YKVHAAGAPGVGKSALARIFGGVE---DGPEAEAAGHTYDRSITVDGEEASLMVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRARRQTDVPIILVGNKSDLVRSREVSVDEGRACAEVFDCKFIETSAALHHNVQALFEGVVRQI-----RLRRDSKED 258
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Match: sp|Q6DGN0|RERGL_DANRE (Ras-related and estrogen-regulated growth inhibitor-like protein OS=Danio rerio OX=7955 GN=rergl PE=2 SV=1) HSP 1 Score: 83.1889 bits (204), Expect = 6.068e-17 Identity = 55/157 (35.03%), Postives = 84/157 (53.50%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTV---TIDLDNREWNLTFLDTVRDEDDDPNNI--QIYHNADGYMIVYAIDEYDSFEYAKIILNYIR------CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTA 1299
+ V+G+ VGKS++ +FLT ++G E A+ S + + +D R+ NL D D + + QI H ADG+++VY I + SF AK I++ IR + DS +FLV NK DL RE+ E+G+ LA + C+F E+S A
Sbjct: 4 IKVAVLGSEGVGKSALIVRFLTRRFIG-----EYASSSECIYRKRMSIDARQLNLELHDPCSQPCDGKSTLNEQI-HWADGFVVVYDISDRSSFLTAKAIVHLIRELHLGATKRDSDSVIFLVGNKQDLCHMREVDREEGQKLASESRCQFYELSAA 154
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Match: sp|O88667|RAD_MOUSE (GTP-binding protein RAD OS=Mus musculus OX=10090 GN=Rrad PE=1 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 1.190e-16 Identity = 57/180 (31.67%), Postives = 94/180 (52.22%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDD---PNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKRED 1389
+ + ++GAP VGKS++ F E + E A + +I +D E +L D + +ED P + D Y+IVY+I + SFE A ++ + R D + LV NKSDL R RE+S ++G+ A +DC+F+E S AL+H V L ++ +I + +++ +ED
Sbjct: 92 YKVLLLGAPGVGKSALARIFGGIE---DGPEAEAAGHTYDRSITVDGEEASLLVYD-IWEEDGGCWLPGHCMAM--GDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQI-----RLRRDSKED 260
BLAST of GTP-binding protein GEM vs. TrEMBL
Match: A0A1I8IKG0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 169.474 bits (428), Expect = 7.189e-45 Identity = 95/215 (44.19%), Postives = 144/215 (66.98%), Query Frame = 1
Query: 859 SFNIQVVGAPLVGKSSMCEQFLTSE-YLGNSSIPEKANES--RTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEK---------KPKSHTNSITNFFRKHFSKHS 1467
S+ +QV+GAP VGK++MCEQF++SE + +S+ ++ +ES + VT+ LD+ E+++TFLDT R DDP ++ I + D YMIV+A+D SF +AK ILN + E +AL +VANKSDL R RE++ +D K+LA Y+ +F+EISTALNH VD+L+V I+ +I Q+ K +KE+ + ++ EK + +S I NF +KHF+ S
Sbjct: 73 SYRVQVMGAPRVGKTTMCEQFMSSENKVLDSTDDDQLDESQEKFVTVLLDSEEYHMTFLDTERA--DDPKSV-IVMDVDAYMIVFAVDSRRSFNFAKQILNELVSEARKSAALIIVANKSDLVRSREVTRDDAKNLAGVYNAKFIEISTALNHNVDELLVSILGQIKTQQRKMEKERSKLISKEKDGRGLRASMRLRSPRTMIGNFIKKHFTSKS 284
BLAST of GTP-binding protein GEM vs. TrEMBL
Match: A0A267F7W1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034202g1 PE=4 SV=1) HSP 1 Score: 171.014 bits (432), Expect = 4.901e-43 Identity = 95/215 (44.19%), Postives = 144/215 (66.98%), Query Frame = 1
Query: 859 SFNIQVVGAPLVGKSSMCEQFLTSE-YLGNSSIPEKANES--RTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEK---------KPKSHTNSITNFFRKHFSKHS 1467
S+ +QV+GAP VGK++MCEQF++SE + +S+ ++ +ES + VT+ LD+ E+++TFLDT R DDP ++ I + D YMIV+A+D SF +AK ILN + E +AL +VANKSDL R RE++ +D K+LA Y+ +F+EISTALNH VD+L+V I+ +I Q+ K +KE+ + ++ EK + +S I NF +KHF+ S
Sbjct: 320 SYRVQVMGAPRVGKTTMCEQFMSSENKVLDSTDDDQLDESQEKFVTVLLDSEEYHMTFLDTERA--DDPKSV-IVMDVDAYMIVFAVDSRRSFNFAKQILNELVSEARKSAALIIVANKSDLVRSREVTRDDAKNLAGVYNAKFIEISTALNHNVDELLVSILGQIKTQQRKMEKERSKLISKEKDGRGLRASMRLRSPRTMIGNFIKKHFTSKS 531
BLAST of GTP-binding protein GEM vs. TrEMBL
Match: A0A267GKK7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032032g1 PE=4 SV=1) HSP 1 Score: 170.629 bits (431), Expect = 5.798e-43 Identity = 95/215 (44.19%), Postives = 144/215 (66.98%), Query Frame = 1
Query: 859 SFNIQVVGAPLVGKSSMCEQFLTSE-YLGNSSIPEKANES--RTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEK---------KPKSHTNSITNFFRKHFSKHS 1467
S+ +QV+GAP VGK++MCEQF++SE + +S+ ++ +ES + VT+ LD+ E+++TFLDT R DDP ++ I + D YMIV+A+D SF +AK ILN + E +AL +VANKSDL R RE++ +D K+LA Y+ +F+EISTALNH VD+L+V I+ +I Q+ K +KE+ + ++ EK + +S I NF +KHF+ S
Sbjct: 323 SYRVQVMGAPRVGKTTMCEQFMSSENKVLDSTDDDQLDESQEKFVTVLLDSEEYHMTFLDTERA--DDPKSV-IVMDVDAYMIVFAVDSRRSFNFAKQILNELVSEARKSAALIIVANKSDLVRSREVTRDDAKNLAGVYNAKFIEISTALNHNVDELLVSILGQIKTQQRKMEKERSKLISKEKDGRGLRASMRLRSPRTMIGNFIKKHFTSKS 534
BLAST of GTP-binding protein GEM vs. TrEMBL
Match: A0A267DLJ9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002251g1 PE=4 SV=1) HSP 1 Score: 155.221 bits (391), Expect = 1.776e-37 Identity = 81/175 (46.29%), Postives = 124/175 (70.86%), Query Frame = 1
Query: 859 SFNIQVVGAPLVGKSSMCEQFLTSE-YLGNSSIPEKANES--RTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKK 1374
S+ +QV+GAP VGK++MCEQF++SE + +S+ ++ +ES + VT+ LD+ E+++TFLDT R DDP ++ I + D YMIV+A+D SF +AK ILN + E +AL +VANKSDL R RE++ +D K+LA Y+ +F+EISTALNH VD+L+V I+ +I ++++
Sbjct: 323 SYRVQVMGAPRVGKTTMCEQFMSSENKVLDSTDDDQLDESQEKFVTVLLDSEEYHMTFLDTERA--DDPKSV-IVMDVDAYMIVFAVDSRRSFNFAKQILNELVSEARKSAALIIVANKSDLVRSREVTRDDAKNLAGVYNAKFIEISTALNHNVDELLVSILGQIKTSSARWRR 494
BLAST of GTP-binding protein GEM vs. TrEMBL
Match: A0A158QUF3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS6174 PE=4 SV=1) HSP 1 Score: 150.214 bits (378), Expect = 7.417e-36 Identity = 112/346 (32.37%), Postives = 175/346 (50.58%), Query Frame = 1
Query: 568 NLYRVRSLKKTPKGVISKGD---------SVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALR----------LKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSE--YLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYI--RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI--CHQKEKYKKEKREDVTAEKK---------------------PKSHTN---SITNFFRKHFS 1458
N+ RVRS ++T +GV+S+GD +R +Q L + SP + + EA R + N + + S +DS A L + S + +QV+GA VGK++MC QFLTSE Y G S E N TVT+D+D ++ +L +D+ +E+ + + +AD Y++VYAID+ +SF+ A+ I++ I RC+I SA+ LV NKSDL R R +S ++GKHLA Y C F E+S ALNH V++L+V ++ I +Q + ++E+ + + K P+ H+ +I FF+KHFS
Sbjct: 161 NIVRVRSFRRTKEGVVSEGDRDVSVAEELDMRRKLSQELSSPSRRMSKSRSSKI------SPGELKLSDRCEAARSIRKLSFSGGGGGSWSNNPASDHSDEDSV--QTAGLGNVSQPYTVQVLGAEQVGKTTMCTQFLTSESLYDGFDSADE-GNVLETVTVDVDGQQTSLLLIDSNGNEETE---FSVIDSADAYLVVYAIDDRESFKKAQTIISEILGRCKIA--SAIVLVGNKSDLVRARSVSLDEGKHLASVYSCGFYEVSIALNHQVNELLVGLVWAIKAANQHVEEERERLSRIASIKTDAGDGSSSHERTSSLKNKIPFPRHHSGMGKAIKRFFKKHFS 492
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Match: rit1 (Ras like without CAAX 1 [Source:NCBI gene;Acc:103032482]) HSP 1 Score: 83.5741 bits (205), Expect = 5.472e-18 Identity = 53/177 (29.94%), Postives = 90/177 (50.85%), Query Frame = 1
Query: 847 SRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKAN----ESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKE 1362
S S + + ++G VGKS++ QF++ + PE + ++ I +D+ NL LDT + Q +G++I Y+I + SF+ A+ I R D+ + LV NKSDL R++S E+GK LA+++ C F E S A + +D++ ++ +I QKE
Sbjct: 9 SHSREYKLVMLGEGGVGKSAIIMQFISHRF------PEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKELIYRVRRTVDTPVVLVGNKSDLAHLRQVSMEEGKELAREFQCPFFETSAAFRYYIDEVFAALVRQI-RQKE 178
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Match: rit1 (Ras like without CAAX 1 [Source:NCBI gene;Acc:103032482]) HSP 1 Score: 81.2629 bits (199), Expect = 3.042e-17 Identity = 53/177 (29.94%), Postives = 89/177 (50.28%), Query Frame = 1
Query: 847 SRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPE----KANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKE 1362
S S + + ++G VGKS++ QF++ + PE ++ I +D+ NL LDT + Q +G++I Y+I + SF+ A+ I R D+ + LV NKSDL R++S E+GK LA+++ C F E S A + +D++ ++ +I QKE
Sbjct: 16 SHSREYKLVMLGEGGVGKSAIIMQFISHRF------PEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKELIYRVRRTVDTPVVLVGNKSDLAHLRQVSMEEGKELAREFQCPFFETSAAFRYYIDEVFAALVRQI-RQKE 185
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Match: rergla (RERG like [Source:NCBI gene;Acc:103034644]) HSP 1 Score: 79.337 bits (194), Expect = 1.341e-16 Identity = 54/155 (34.84%), Postives = 80/155 (51.61%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTV---TIDLDNREWNLTFLDTVRD--EDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIR------CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTA 1299
+ V+G VGKS++ +FLT ++G E A+ S + + +D R+ NL D E N QI H ADG+++VY I + SF A I++ IR + DS +FLV NK DL RE+ E+G+ +A C+F E+S A
Sbjct: 6 VAVLGGEGVGKSALIVRFLTRRFIG-----EYASSSECIYRKRMSVDGRQLNLELYDPCSQPCEGKSTLNEQI-HWADGFVVVYDISDRSSFLAATAIVHLIRELNLGVAKRDSDSVVFLVGNKQDLCHMREVDREEGQRVASDSRCQFYELSAA 154
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Match: rem1 (GTP-binding protein REM 1 [Source:NCBI gene;Acc:103041805]) HSP 1 Score: 80.4925 bits (197), Expect = 2.349e-16 Identity = 62/172 (36.05%), Postives = 92/172 (53.49%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFL-TSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDE--DDDPNNIQIY--HNADGYMIVYAIDEYDSFEYA---KIILNYIRCE--IPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ + ++G VGKSS+ F E + I E E RT+T+D E L +DT E ++D +Q Y + Y+IVY+I + SFE A +I L IR IP + LV NKSDL R RE++ E+G+ A +DC+F+E S +L+H V +L I+ +I
Sbjct: 78 YRVVLLGDHGVGKSSLASIFAGIQEKDAHHHIGEDTYE-RTLTVD--GEETTLVVMDTWETEKQEEDEKWVQDYCMQVGNAYVIVYSITDRSSFESASELRIQLRRIRQAENIP----IILVGNKSDLVRCREVAVEEGRACAVVFDCKFIETSASLHHNVHELFEGIVRQI 242
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Match: rrad (RRAD, Ras related glycolysis inhibitor and calcium channel regulator [Source:NCBI gene;Acc:103041283]) HSP 1 Score: 78.9518 bits (193), Expect = 9.375e-16 Identity = 51/163 (31.29%), Postives = 82/163 (50.31%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ + ++G VGKSS+ F E ++ E A + +I +D E N+ D ++ Q D Y+IVY++ + SFE A ++ + R + + LV NKSDL R RE+S E+G A +DC+F+E S +L+H V L I+ +I
Sbjct: 91 YKVVLLGEHGVGKSSLARIFGGVE---DNHDCEDAGNTYDRSITVDEEEANILLYDVWEQDNSQWLKDQCMKMGDAYIIVYSVTDKSSFEKASELRIQLRRARQSENIPIILVGNKSDLVRSREVSVEEGSACAVVFDCKFIETSASLHHNVRTLFEGIVRQI 250
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Match: RIT2 (Ras like without CAAX 2 [Source:HGNC Symbol;Acc:HGNC:10017]) HSP 1 Score: 77.7962 bits (190), Expect = 1.194e-16 Identity = 49/171 (28.65%), Postives = 89/171 (52.05%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKAN----ESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA---KIILNYIR--CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ I ++G VGKS+M QF++ + PE + ++ + +D+ +L LDT + Q +G++I Y+I + SF+ A K ++ +R E+P + LV NK+DL R++S E+G+ LA+++ C+F E S AL VD++ ++ +I
Sbjct: 21 YKIVMLGPGGVGKSAMTMQFISHRF------PEDHDPTIEDAYKTQVRIDDETAHLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFQEAAQFKQLIYQVRHTYEVP----VVLVGNKTDLGEIRQVSQEEGRSLAREFSCQFFETSAALRQYVDEVFYAVVREI 181
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Match: ralba (v-ral simian leukemia viral oncogene homolog Ba (ras related) [Source:ZFIN;Acc:ZDB-GENE-040801-267]) HSP 1 Score: 73.559 bits (179), Expect = 2.714e-15 Identity = 55/185 (29.73%), Postives = 94/185 (50.81%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKP 1410
+ +VG+ VGKS++ QF+ E++ + P KA+ R + LD E + LDT ED + + +G++ V++I E++SF E+ + IL + P LV NKSDLE R++SSE+ + A+Q+ ++E S VD K+ + K K ++ +D + + KP
Sbjct: 16 VIMVGSGGVGKSALTLQFMYDEFVEDYE-PTKADSYRKKVV-LDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSICEHESFAATLEFREQILRVKEDDSVP---FLLVGNKSDLEERRQVSSEEARARAEQWRVSYVETSAKTRANVD----KVFFDLMRDVRKRKMDEGKDKSGKVKP 191
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Match: rheb (Ras homolog, mTORC1 binding [Source:ZFIN;Acc:ZDB-GENE-040426-1690]) HSP 1 Score: 68.1662 bits (165), Expect = 1.332e-13 Identity = 53/153 (34.64%), Postives = 84/153 (54.90%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIY-HNADGYMIVYAIDEYDSFEYAKII----LNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNH 1308
I V+G VGKSS+ QF+ +++ +S P N + T + ++ +E+NL +DT +D+ Q Y + GY++VY++ SFE ++I L+ + + +P L LV NK DL R IS E+GK LA ++ F+E STA H
Sbjct: 9 IAVLGYRSVGKSSLTIQFVEGQFV-DSYDPTIEN-TFTKNLTVNGQEFNLKLVDTA-GQDEYSMFPQTYSMDIHGYVLVYSVTSIKSFEVVRVIHEKLLDMVGKVHVP----LVLVGNKKDLHMERVISCEEGKTLATSWNAAFME-STAKEH 153
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001560.1 (pep scaffold:Pmarinus_7.0:GL476528:157506:173211:1 gene:ENSPMAG00000001407.1 transcript:ENSPMAT00000001560.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 1.488e-12 Identity = 45/163 (27.61%), Postives = 80/163 (49.08%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIIL---NYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ +VG+ VGKS++ QF+ E++ + P KA+ R + LD E + LDT ED + + +G++ ++I E++SF + +R + LV NKSDLE R++S E+ + A Q+ ++E S VD + ++ +I
Sbjct: 17 VIMVGSGGVGKSALTLQFMYDEFVEDYE-PTKADSYRKKVV-LDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCAFSICEHESFAGTTDLRRSEQILRVKEDDSIPFLLVGNKSDLEERRQVSIEEARARADQWGVGYVETSAKTRANVDKVFFDLMREI 177
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002219.1 (pep scaffold:Pmarinus_7.0:GL483980:50:9020:-1 gene:ENSPMAG00000002006.1 transcript:ENSPMAT00000002219.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 61.6178 bits (148), Expect = 3.904e-11 Identity = 43/163 (26.38%), Postives = 74/163 (45.40%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKAN-ESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
F ++G VGKS + QF +++ + P E T I++ ++ L DT E Y A G ++VY I ++ + L R P++ +FL+ NK+DLE R+++ E+ K A++ FLE S V++ ++ KI
Sbjct: 12 FKYIIIGDMGVGKSCLLHQFTEKKFMADC--PHTIGVEFGTRIIEVSGQKVKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIFLIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGDNVEEAFLETAKKI 172
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Match: YPT32 (Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication [Source:SGD;Acc:S000003178]) HSP 1 Score: 64.3142 bits (155), Expect = 3.561e-12 Identity = 55/198 (27.78%), Postives = 89/198 (44.95%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKAN---ESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVD----DLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSIT 1434
F I ++G VGKS++ +F T E+ +I K+ E T TI+++N++ DT E Y A G +IVY I + S+E L +R + A+ L+ NKSDL R + +++ K+ A + F E S + VD +LIV I + + ++ + PK T S+T
Sbjct: 14 FKIVLIGDSGVGKSNLLSRFTTDEF----NIESKSTIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNSDNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLT 207
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Match: RSR1 (GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases [Source:SGD;Acc:S000003384]) HSP 1 Score: 61.2326 bits (147), Expect = 6.519e-11 Identity = 45/166 (27.11%), Postives = 82/166 (49.40%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF-EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYD-CEFLEISTALNHMVDDLIVKIIHKICH 1353
+ + V+GA VGKS + QF+ YL ++ P + R TI++DN+ ++L LDT + G+++VY++ + S E ++ +R + + L+ NK+DL R IS E+G ++ ++ F E S L VD++ V ++ +I
Sbjct: 4 YKLVVLGAGGVGKSCLTVQFVQGVYL-DTYDPTIEDSYRK-TIEIDNKVFDLEILDTAGIAQFTAMRELYIKSGMGFLLVYSVTDRQSLEELMELREQVLRIKDSDRVPMVLIGNKADLINERVISVEEGIEVSSKWGRVPFYETSALLRSNVDEVFVDLVRQIIR 167
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Match: RAS1 (GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication [Source:SGD;Acc:S000005627]) HSP 1 Score: 60.8474 bits (146), Expect = 1.026e-10 Identity = 54/183 (29.51%), Postives = 88/183 (48.09%), Query Frame = 1
Query: 898 KSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDS---ALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKRE-DVTAEKKPKSHTNSIT 1434
KS++ QF+ S Y + P + R + +D++ L LDT E+ Q +G+++VY++ +SF+ +++ Y + + DS + +V NK DLE R++S EDG LAKQ + FLE S VD+ +I + KY R+ D T E + T+S T
Sbjct: 23 KSALTIQFIQS-YFVDEYDPTIEDSYRKQVV-IDDKVSILDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRNSFD--ELLSYYQQIQRVKDSDYIPVVVVGNKLDLENERQVSYEDGLRLAKQLNAPFLETSAKQAINVDEAFYSLIRLVRDDGGKYNSMNRQLDNTNEIRDSELTSSAT 201
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Match: YPT31 (Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi [Source:SGD;Acc:S000000833]) HSP 1 Score: 58.9214 bits (141), Expect = 1.881e-10 Identity = 54/196 (27.55%), Postives = 85/196 (43.37%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVD----DLIVKIIHKIC-HQKEKYKKEKREDVTAEKKPKSHTNSIT 1434
F I ++G VGKS++ +F +E+ +S E T T+++D + DT E Y A G +IVY I + S+E L+ +R + A+ L+ NKSDL R + +E+ K A++ F E S + VD +LI I K+ HQ + + P T S+T
Sbjct: 14 FKIVLIGDSGVGKSNLLSRFTKNEFNMDSK-STIGVEFATRTLEIDGKRIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLSELRENADDNVAVGLIGNKSDLAHLRAVPTEESKTFAQENQLLFTETSALNSENVDKAFEELINTIYQKVSKHQMDLGDSSANGNANGASAPNGPTISLT 208
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Match: RAS2 (GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication [Source:SGD;Acc:S000005042]) HSP 1 Score: 56.9954 bits (136), Expect = 2.337e-9 Identity = 41/154 (26.62%), Postives = 71/154 (46.10%), Query Frame = 1
Query: 1015 LTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFE----YAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKH 1464
L LDT E+ Q N +G+++VY+I S + Y + IL + P + +V NKSDLE +++S +DG ++AKQ + FLE S V++ + + + KY K E+ +++ + S N ++ H
Sbjct: 60 LDILDTAGQEEYSAMREQYMRNGEGFLLVYSITSKSSLDELMTYYQQILRVKDTDYVP---IVVVGNKSDLENEKQVSYQDGLNMAKQMNAPFLETSAKQAINVEEAFYTLARLVRDEGGKYNKTLTENDNSKQTSQDTKGSGANSVPRNSGGH 210
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Match: EDO36396 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIQ7]) HSP 1 Score: 84.7297 bits (208), Expect = 5.880e-19 Identity = 57/195 (29.23%), Postives = 100/195 (51.28%), Query Frame = 1
Query: 877 VGAPLVGKSSMCEQFLTSEYLG-NSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTA--EKKPKSHTNSITNFFRK 1449
+G VGKS++ Q ++ ++L + E A + + V LDN L LDT ++ Q N +G++I+Y+I + SF A + I R D + LV NK DLE RE+++++G+ LA ++DC F E S H VDD ++ +I +K++++ +T +++ K S+ NF ++
Sbjct: 6 LGGGGVGKSALIVQLISHQFLDYHDPTIEDAYQHQVV---LDNEPALLDILDTAGQQEFTAMREQYMRNGEGFIIIYSITDRSSFNLAAQYKDQIERVRRTDDIPIVLVGNKDDLEGKREVTTQEGQELAHRFDCPFFETSACQRHFVDDAFHGLVREI-------RKKEKQRLTGHFQEEKKGLVKSLRNFVKR 190
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Match: EDO40041 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8C2]) HSP 1 Score: 69.3218 bits (168), Expect = 6.099e-14 Identity = 44/159 (27.67%), Postives = 80/159 (50.31%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIR--------CEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMV 1314
+++ V+GA VGK+++ +F+T +L + + + I + E ++ LDT + + HN DG+++VY+I +Y S+E A+ ++ + C+IP + LV NK DL RGR ++ ++ + A +Y C E S N V
Sbjct: 2 YSMVVLGAAGVGKTALVVRFVTGRFLNEYDPTLEMTYEKELRIGDSSMELDI--LDTAGNGEA-----SYIHNGDGFVVVYSITDYYSYEMARQLVKLVTQVRKTDDICQIP----IVLVGNKRDLRRGRCVAKDEAREFASEYGCSHYETSALTNRNV 149
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Match: EDO45827 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRV2]) HSP 1 Score: 68.5514 bits (166), Expect = 2.185e-13 Identity = 50/168 (29.76%), Postives = 86/168 (51.19%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQK 1359
+ +VG+ VGKS++ QF+ E++ + P KA+ R I LD E + LDT ED + + +G++ V++I E +SF E+ + IL R + LV NKSDL R+++SE+G+ A ++ ++E S VD + ++ +I +K
Sbjct: 14 VIMVGSGGVGKSALTLQFMYEEFVEDYE-PTKADSYRKKAI-LDGEECQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEPESFAATTEFREQIL---RVKGDDKIPFLLVGNKSDLTDKRQVTSEEGQSKADEWGVPYVETSAKTRSNVDKVFFDLMREIRSRK 176
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Match: EDO30839 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZM4]) HSP 1 Score: 64.6994 bits (156), Expect = 3.315e-12 Identity = 53/200 (26.50%), Postives = 94/200 (47.00%), Query Frame = 1
Query: 862 FNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSAL----FLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRK 1449
+++ ++G VGKS++ +F T ++ + + TID D ++T D + + N +G+++VY+I+E +FE AK ++ IR EI AL L NK DL+ R +S ++ + A +++C F E S A +++ K+I C ++ R K+ NSI +FF K
Sbjct: 4 YSLVLLGQAGVGKSALLVRFCTGRFIHEYDPTLEMTYDVSATIDEDPATLHIT------DTASTYEPVYLSQN-EGFIVVYSINELRTFETAKQLVKLIR-EIKKQKALTTLIVLAGNKCDLKHCRAVSRQEAREFAAEHECVFHETSAA-----NNINTKVIFHDC------VRQIRSTHLMNKRANGAMNSIKHFFSK 184
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Match: EDO46259 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQA3]) HSP 1 Score: 64.3142 bits (155), Expect = 4.012e-12 Identity = 46/175 (26.29%), Postives = 90/175 (51.43%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDS-ALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNH-MVDDLIVKIIHKICHQKEKYKKEKRE 1386
+ V+G VGKS++ +FLT ++G ++ + ID N + +L DT + D + D Y+++Y+I + SF+ A+ I Y++ DS ++ +VA K DL+ R + E+G + + DC F E+S + + V +L+ +++ +I + + EK++
Sbjct: 11 VVVLGKDGVGKSALTVRFLTRRFIGEYDQTLESTHRHILQID--NEDVSLDISDTAGEVRDKLR--RWTRRGDLYLVLYSIVDRSSFDEARWIARYVKDHRNTDSMSMVIVATKKDLDHLRRVDIEEGLGFSNELDCTFYEVSISEGYNEVQELLHELLKRITRNRGEKGTEKKD 181
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Match: rasl11a (RAS like family 11 member A [Source:NCBI gene;Acc:101169318]) HSP 1 Score: 79.337 bits (194), Expect = 3.274e-16 Identity = 59/197 (29.95%), Postives = 100/197 (50.76%), Query Frame = 1
Query: 859 SFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDT-VRDEDDDPNNI----QI---YHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDS--ALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNH-MVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKS 1416
+ I V+GA VGK+++ +FLT ++G+ A SR VT+D++ E +L DT D+P + QI + ADGY++V++I ++ S+ + + ++R I P + LV NKSDL R R++S+++G+ LA + E+S NH V + + ++ EKR +PKS
Sbjct: 35 TVKIAVLGASNVGKTALIVRFLTKRFIGDYEANTGALYSRKVTLDVE--EVSLQVQDTPCVALQDNPEGLYCQEQINRSIYWADGYVLVFSITDHSSYRTIQPLYQHVR-RIHPSGNIPVILVGNKSDLLRARQVSADEGETLAASLGGVYFEVSARENHEGVHAAFLHLCQEVVRALGGGNGEKRRGGLHLARPKS 228
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Match: rrad (RRAD, Ras related glycolysis inhibitor and calcium channel regulator [Source:NCBI gene;Acc:101173131]) HSP 1 Score: 79.7221 bits (195), Expect = 4.893e-16 Identity = 52/178 (29.21%), Postives = 92/178 (51.69%), Query Frame = 1
Query: 853 STSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKR 1383
S + + ++G VGKSS+ F E +G+ ++A + ++ +D E ++ D ++ Q D Y+IVY++ + SFE A ++ + R + + LV NKSDL R RE+S ++G A +DC+F+E S +L+H V DL I+ +I +K+ ++ R
Sbjct: 89 SQVYKVVLLGEHGVGKSSLARVFGGVEDVGHDY--DEAGNTYDRSLVVDEEEASILLYDIWEQDNSQWLKDQCMRMGDAYIIVYSVTDKSSFEKASELRIQLRRARQSENIPIILVGNKSDLVRSREVSVDEGSACAVVFDCKFIETSASLHHNVQDLFEGIVRQIRLRKDSKEENAR 264
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Match: ENSORLT00000001678.2 (ras-related protein O-RAL [Source:NCBI gene;Acc:101156149]) HSP 1 Score: 74.3294 bits (181), Expect = 7.072e-15 Identity = 49/164 (29.88%), Postives = 86/164 (52.44%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
+ +VG+ VGKS++ QF+ E++ + P KA+ R + LD + + LDT ED + + +G+++V++I E +SF E+ + IL E P L LV NKSDLE R+I++ED A ++ +++E S VD + ++ ++
Sbjct: 17 VIMVGSGGVGKSALTLQFMYDEFVEDYE-PTKADSYRKKVV-LDGEDVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITELESFTATSEFREQILRVKEEEAIP---LLLVGNKSDLEDRRQITAEDAASKAGEWGVQYVETSAKTRANVDKVFFDLMREV 175
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Match: ENSORLT00000029945.1 (GTP-binding protein Rheb [Source:NCBI gene;Acc:101160603]) HSP 1 Score: 73.1738 bits (178), Expect = 1.044e-14 Identity = 52/167 (31.14%), Postives = 91/167 (54.49%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDD--PNNIQIYHNADGYMIVYAIDEYDSFEYAKII----LNYI-RCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
I V+G VGKSS+ QF+ +++ +S P N + T T+ + +E+NL +DT ++ P + I + DGY+++Y++ + SFE ++I L+ + ++P + LV NK DL R IS EDG+ LA ++ FLE S N ++ ++I ++
Sbjct: 9 IAVLGYRSVGKSSLTIQFVEGQFV-DSYDPTIEN-TFTTTMTVSGQEYNLQLVDTAGQDEYSIFPQSYTI--DVDGYILIYSVTSFKSFEVIRVIHEKLLDMVGNVQVP----ITLVGNKKDLHMERMISLEDGRALADSWNAAFLESSAKENQTAVEVFRRMILEV 167
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Match: ralba (ras-related protein Ral-B [Source:NCBI gene;Acc:101157118]) HSP 1 Score: 72.7886 bits (177), Expect = 2.333e-14 Identity = 50/169 (29.59%), Postives = 89/169 (52.66%), Query Frame = 1
Query: 868 IQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSF----EYAKIILNYIRCEIPPDSA-LFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQK 1359
+ +VG+ VGKS++ QF+ E++ + P KA+ R + LD E + LDT ED + + +G+++V++I E +SF E+ + IL R + D L LV NKSDLE R +S E+ + A+++ +++E S VD + ++ ++ +K
Sbjct: 17 VIMVGSGGVGKSALTLQFMYDEFVEDYE-PTKADSYRKKVV-LDGDEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEQESFTATDEFREQIL---RVKADEDKIPLLLVGNKSDLEERRHVSVEEARRKAEEWGAQYVETSAKTRANVDKVFFDLMREVRGKK 180
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Match: SMESG000032890.1 (SMESG000032890.1) HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0 Identity = 397/403 (98.51%), Postives = 401/403 (99.50%), Query Frame = 1
Query: 280 MDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLPSDFESSSREELPCENRRDSLQPSSFRNRSIKENRSHSSLRQTPEIFEIADEGSQNLYRVRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHSQSTIDNH 1488
MDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLP+DFESSSREELPCENRRDSLQPSSFRNRSIKENRSHSSLRQTPEIFEIA EGSQNLYRVRSLKKTPKGVISKGD VRNHSNQSLEKEFVYPCTEHHQSLNSS+VNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKK+KRE+VTAEKKPKSHTNSITNFFRKHFSKHSQSTIDNH
Sbjct: 1 MDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLPTDFESSSREELPCENRRDSLQPSSFRNRSIKENRSHSSLRQTPEIFEIAGEGSQNLYRVRSLKKTPKGVISKGDLVRNHSNQSLEKEFVYPCTEHHQSLNSSKVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKDKREEVTAEKKPKSHTNSITNFFRKHFSKHSQSTIDNH 403
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Match: SMESG000018613.1 (SMESG000018613.1) HSP 1 Score: 167.548 bits (423), Expect = 6.293e-47 Identity = 115/303 (37.95%), Postives = 163/303 (53.80%), Query Frame = 1
Query: 571 LYRVRSLKKTPKGVISKGDSVRNHSNQSL-----EKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLG---NSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVTAEKKPKSHT--NSITNFFRK 1449
LYR+RS K+T KGVI++GD+V+N S SL K F + N R N+ +E+ + N++ ++ K T+F++ V+G VGK+++CEQFLTSE G N P+K R VT+DLD+ EW L D D + + D Y+IVY+I++ DSF++ IL + E SA+F+VA KSDLER R++S E GK LA YD EF+E+ST LNHM+D+L+V+II KI K+K +K K K K T N +FFR+
Sbjct: 71 LYRIRSFKRTHKGVINQGDTVKNCSLLSLNLPTRSKSFT-------KGENPYRYNNNDKSPISSPEESHSIDLLVPQNINEQK-------------KEIKTTFDVFVIGKNGVGKTALCEQFLTSETYGFYSNMDAPDKT--IRQVTVDLDDNEWTLVLHDL------DNISTTFEESPDAYIIVYSIEDKDSFDWCTQILQNL-LESLSTSAIFIVATKSDLERLRQVSCEKGKRLASIYDAEFIEVSTVLNHMIDNLLVQIITKIRRHKKKLRKAKDSKTNYSMKHKKSTPGNLFMDFFRR 344
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Match: SMESG000060724.1 (SMESG000060724.1) HSP 1 Score: 152.14 bits (383), Expect = 1.488e-40 Identity = 106/354 (29.94%), Postives = 173/354 (48.87%), Query Frame = 1
Query: 370 SPYRIRSQSLL-PSDFES-SSREELPCENRRDSLQP-----SSFRNR--SIKENRSHSSL--RQTPEIFEIADEGSQNLYRVRSLKKTPKGVISKGDSVRNHSNQSLEK---------------EFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPN--NIQIYHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKI 1347
SP +R+ L+ ++F S S ++ +RR + P +F N+ S+ +N+ + +L Q +++D+G +YR+RS K+T KG++S+ D+++ S SLEK EF +PC RL + P + +Q++G+ VGK++M QF+TSE++ + + V++ D++EWN+ DT +D D N +++ +GYM+VYAID SF Y K IL + + DS + LV NK DLER RE+S E GK LAK + C F E+STA+NH +D+L+++ + KI
Sbjct: 74 SPNHLRNPDLIIKTNFRSRSMKDRCLTGHRRSTYVPLVESEQNFLNKKDSLTKNKEYKTLVWSQNENQKDLSDDGEIQMYRMRSFKRTSKGIVSQADTIKARSINSLEKFDPLEDSKWDKNESEEFHFPC--------------------------YRLGISCGPE------------------QKIVNRLEVQIIGSNFVGKTTMASQFMTSEHICGRMDSVNEDADKFVSVFFDDQEWNIWLHDTENKKDADSNEVTVELLSGMNGYMVVYAIDNMSSFIYTKHILTDLVEKASRDSVIALVGNKIDLERCREVSRESGKQLAKIHRCLFYEVSTAINHKIDELLIQTLMKI 383
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Match: SMESG000057130.1 (SMESG000057130.1) HSP 1 Score: 138.658 bits (348), Expect = 5.647e-36 Identity = 119/417 (28.54%), Postives = 207/417 (49.64%), Query Frame = 1
Query: 280 MDEFEYYCQLARS-TSNKNDNRNFLNPKPLESPYRIRS-QSLLPSDFESSSREELPCE----NRRDSLQPSSFRNRSIKENRSHSSLRQTPE---IFEIADEGSQN-LYRVRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKA-NESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQIYHNADGYMIVYAIDEYDSFEYA-KIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEKYKKEKREDVT---------AEKKPKSHTNSITNFFRKHFSKHS 1467
+D+F+ +C++A++ S N RN+ P + YR S + +P + E L N ++ SFR + + S L PE + G +N + R+RS ++T KG++ D++ S + L + F + N S N F+ +K E L+ +PN D +I + + + G VGK+S+C+Q L EYL + + K+ NE++ + + +DN+E L +D+ ++ N I+ +D Y+++YAID+ SFE K+I N + + P + LVANKSDL R R +S++ GK LA Y+ +F EIST+LNH VD+L+V ++ ++ + ++ +K K ++ + K K N+++ F +KHF+K S
Sbjct: 6 LDDFDRFCRIAQNPISETNKYRNYNLPGIESTLYRSNSVRERVPKTLKEIKTEPLSSHPNSYNENTVIRARSFRRINKGDMSSRDPLVIEPEKDQLLSNVSSGEENKIVRLRSFRRTTKGIVCNKDTIEIESEKELSRSF--------DTRNLSNNNEHFHFKNDRKSE---LENQKLPN--------DGEI----------IRYTVTLFGQDGVGKTSLCQQLLLPEYLVSQADEIKSHNEAQMIRVLIDNQESILRLIDS----ENCENFIEPKEVSDAYLVIYAIDDNSSFETCRKLIANLVLTNVNP-PLVILVANKSDLVRSRVVSTDKGKRLAGIYNLKFKEISTSLNHCVDELLVLLVTEMRNASKRTEKHKVNFISKGFQNTPYRSSLKIKVSNNAVSRFIQKHFNKSS 388
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Match: SMESG000019070.1 (SMESG000019070.1) HSP 1 Score: 129.798 bits (325), Expect = 5.832e-33 Identity = 90/306 (29.41%), Postives = 144/306 (47.06%), Query Frame = 1
Query: 580 VRSLKKTPKGVISKGDSVRNHSNQSLEKEFVYPCTEHHQSLNSSRVNSPKSFETKKKKEALRLKQTTMPNVSFERSRDDSQIHWHANLKSRSTSFNIQVVGAPLVGKSSMCEQFLTSEYLGNSSIPEKANESRTVTIDLDNREWNLTFLDTVRDEDDDPNNIQI--YHNADGYMIVYAIDEYDSFEYAKIILNYIRCEIPPDSALFLVANKSDLERGREISSEDGKHLAKQYDCEFLEISTALNHMVDDLIVKIIHKICHQKEK--------YKKEKREDVTAEKKPKSHTNSITNFFRKHFSKHS 1467
VRS K T KG+I++GD + + S +++ E ++ +QS S ++SP IQ++GA VGK+++ QF+TSE + + + +I +D +EWN+ DT D N +++ + Y++++AID+ SFEY+K +L I ++ A +LV NK+DLER RE+ S+ K L+K + C F EISTALNH VD+L+ KII I + K YK+ + + VT + ++ RK F S
Sbjct: 116 VRSFKVTEKGMINEGDLLISRSTGNIKSENIHVQYNGNQSDKYSNIDSP---------------------------------------------LKIQILGANGVGKTTLASQFMTSEQICCRMMSVNEETEKLFSIRIDGKEWNILLHDTCNMNIDATNEVRVELLDKMNAYIVLFAIDDLASFEYSKQVLKDIFKKVDTTRAAYLVGNKADLERSREVKSKICKELSKVHRCHFYEISTALNHKVDELLCKIIMDILKSQSKVESPRKSIYKRRESQLVTTGR-------ALGKLLRKTFGTKS 369 The following BLAST results are available for this feature:
BLAST of GTP-binding protein GEM vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of GTP-binding protein GEM vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of GTP-binding protein GEM vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of GTP-binding protein GEM vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006233 ID=SMED30006233|Name=GTP-binding protein GEM|organism=Schmidtea mediterranea sexual|type=transcript|length=1577bpback to top protein sequence of SMED30006233-orf-1 >SMED30006233-orf-1 ID=SMED30006233-orf-1|Name=SMED30006233-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=404bp MDEFEYYCQLARSTSNKNDNRNFLNPKPLESPYRIRSQSLLPSDFESSSRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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