SMED30006223
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006223 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Alignments
SMED30006223 aligns in the following genomic locations:
Homology
BLAST of SMED30006223 vs. Planmine SMEST
Match: SMESG000003494.1 (SMESG000003494.1) HSP 1 Score: 258.455 bits (659), Expect = 8.798e-80 Identity = 148/401 (36.91%), Postives = 233/401 (58.10%), Query Frame = 1 Query: 1 VIIQLIGKFSCGLIGEFKYKFENHLKFEIFIDF---LPIADKNLVQLIDSDQNRLLIQTSRSFAHDNKLIINFNGFTYNWISYQSFSLFYYKCC*FLTPDRRYQDIFFLDIKMKLINNKLYKLGSSSYVLLFPD--VFNGNGSKRMTEND------SIRQRNLAKVWLGHERNFINYSNSDWPTNQIIGCVHRFRLNNKKINFAKYGSNFFANCILQQSERICQDSECRNGT-SFLHILDCSLAKVNTGRHCFPGLYNNVNKFYSYAPIINEPIRIKLAWIPKLSISQCLLEIGAKNDSIISIYVGPTGKIVLNIKHNKLTEVPKFILHRMTSNDIYVILIKFTVRKWEIQVSDGIFIRAQEVLDLQGVHFINIGWNTNVNFCQPLNDKFEIQHNINVKSG 1167 +++ ++G S LIGE + +F N L I I + +DK + IDS++N+L I+TS+ FA+D+KLIINFNG T +Y+ RY +F+L IK+ N LY S L+ D + + S+ END + N K+WLG++ ++I S+SDWPTNQIIGC +R +N K + +G+NF+++C + +E IC+ S+CRN T ++ HILDC L++++T HC+P + +N +SY PI +E + +KL WIP+LSISQCL+ I N+SII IY+ +I+L + K + K+ ++++S DI+ + I F I++S+G FI+ + + + + + I IGWN N C P+ +KF IQ N N+KS Sbjct: 10 ILVSILGILSSYLIGEIEEEFRNDLIIWIKIRYGIRSQTSDKYFLHAIDSNRNKLSIKTSKLFANDSKLIINFNGQTGTRTAYKYI---------------RYVGVFYLHIKLTFKANNLYAGKWSPDELIISDELISYSSSSRAAMENDWPSAGDVSKLTNRMKIWLGYDHDWIGKSHSDWPTNQIIGCGYRIDVNKKSVILVNHGNNFYSDCFNEVTESICEQSQCRNETDNYQHILDCRLSRMSTAPHCYPDVTDNTEGLHSYQPIKDEHVTVKLGWIPQLSISQCLMHIITSNESIIDIYINSANQIILEMTIKKSIK-SKYNSYQISSIDIHALSIGFIDHNLTIRISNGSFIKTRMLPNQERIKTIYIGWNPNPCLCHPIRNKFGIQLNSNLKSA 394
BLAST of SMED30006223 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 252.292 bits (643), Expect = 1.168e-77 Identity = 146/394 (37.06%), Postives = 230/394 (58.38%), Query Frame = 1 Query: 1 VIIQLIGKFSCGLIGEFKYKFENHLKFEIFIDFLPIADKNLVQLIDSDQNRLLIQTSRSFAHDNKLIINFNGFTYNWISYQSFSLFYYKCC*FLTPDRRYQDIFFLDIKMKLINNKLYKLGSSSYVLLFPDVFNGNGSKR-MTEND---SIRQRNLAKVWLGHERNFINYSNSDWPTNQIIGCVHRFRLNNKKINFAKYGSNFFANCILQQSERICQDSECRNGT-SFLHILDCSLAKVNTGRHCFPGLYNNVNKFYSYAPIINEPIRIKLAWIPKLSISQCLLEIGAKNDSIISIYVGPTGKIVLNIKHNKLTEVPKFILHRMTSNDIYVILIKFTVRKWEIQVSDGIFIRAQEVLDLQGVHFINIGWNTNVNFCQPLNDKFEIQHNINVKSG 1167 +++ + G S LIGE + +F N + I I F I+DK L+ ID++QN+L I+TS+SFA D+KLI+NFNG T +KC + N LY S L+ ++ + + R +TEND + N K+WLG++ ++I+ S+S+WPTNQIIGCV +N K I F K+G++F+ +C + +E IC+ S+CRN T + +IL+C L ++T HC+P + +N +SY PI + + +K+AWIP+LSI QCL++I +NDSII IY+ +I++ I NK + K+ ++++S D++ + + F K I++S+G FI+ + + + V INIGWN+N CQP+ KF IQ N N+KS Sbjct: 10 ILVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATR-----------AFKC----------------------LENSLYAAKWPSDELIISNISYSSINSRSVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMDCYNRVTESICEKSQCRNETDNHQYILNCRLPGLSTSPHCYPDITDNTGGLHSYQPITHGHVAVKIAWIPQLSIRQCLIQIITRNDSIIDIYINSANQIMIEITDNKSIKSSKYNSYQISSTDVHALSMGFIDHKLTIRISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSA 370
BLAST of SMED30006223 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 252.292 bits (643), Expect = 1.448e-77 Identity = 147/394 (37.31%), Postives = 230/394 (58.38%), Query Frame = 1 Query: 1 VIIQLIGKFSCGLIGEFKYKFENHLKFEIFIDFLPIADKNLVQLIDSDQNRLLIQTSRSFAHDNKLIINFNGFTYNWISYQSFSLFYYKCC*FLTPDRRYQDIFFLDIKMKLINNKLYKLGSSSYVLLFPDVFNGNGSKR-MTEND---SIRQRNLAKVWLGHERNFINYSNSDWPTNQIIGCVHRFRLNNKKINFAKYGSNFFANCILQQSERICQDSECRNGT-SFLHILDCSLAKVNTGRHCFPGLYNNVNKFYSYAPIINEPIRIKLAWIPKLSISQCLLEIGAKNDSIISIYVGPTGKIVLNIKHNKLTEVPKFILHRMTSNDIYVILIKFTVRKWEIQVSDGIFIRAQEVLDLQGVHFINIGWNTNVNFCQPLNDKFEIQHNINVKSG 1167 V++ + G S LIGE + +F N + I I F I+DK L+ ID++QN+L I+TS+SFA D+KLI+NFNG T +KC + N LY S L+ ++ + + R +TEND + N K+WLG++ ++I+ S+S+WPTNQIIGCV +N K I F K+G++F+ +C + +E IC+ S+CRN T + +IL+C L ++T HC+P + +N +SY PI + + +K+AWIP+LSI QCL++I +NDSII IY+ +I++ I NK + K+ ++++S D++ + + F K I++S+G FI+ + + + V INIGWN+N CQP+ KF IQ N N+KS Sbjct: 23 VLVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATR-----------AFKC----------------------LENSLYAAKWPSDELIISNISYSSINSRSVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMDCYNRVTESICEKSQCRNETDNHQYILNCRLPGLSTSPHCYPDITDNTGGLHSYQPITHGHVAVKIAWIPQLSIRQCLIQIITRNDSIIDIYINSANQIMIEITDNKSIKSSKYNSYQISSTDVHALSMGFIDHKLTIRISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSA 383
BLAST of SMED30006223 vs. Planmine SMEST
Match: SMESG000003551.1 (SMESG000003551.1) HSP 1 Score: 251.136 bits (640), Expect = 6.980e-77 Identity = 148/415 (35.66%), Postives = 235/415 (56.63%), Query Frame = 1 Query: 4 IIQLIGKFSCGLIGEFKYKFENHLKFEIFIDFLPIADKNLVQLIDSDQNRLLIQTSRSFAHDNKLIINFNGFTYNWISYQSFSLFYYKCC*FLTPDRRYQDIFFLDIKMKLINNKLY------------KLGSSSYVLLFPDVFNGNGSKRMTENDSIRQRNLAK------VWLGHERNFINYSNSDWPTNQIIGCVHRFR--------LNNKKINFAKYGSNFFANCILQQSERICQDSECRNGT-SFLHILDCSLAKVNTGRHCFPGLYNNVNKFYSYAPIINEPIRIKLAWIPKLSISQCLLEIGAKNDSIISIYVGPTGKIVLNIKHNKLTEVPKFILHRMTSNDIYVILIKFTVRKWEIQVSDGIFIRAQEVLDLQGVHFINIGWNTNVNFCQPLNDKFEIQHNINVKSG 1167 ++ ++G S LIGE + +F N LK I I+F I+DK L+Q IDS+ N+L I+TS+SFA+DN++IINFNG T +Y+ + + RY +F+L +++ N LY KL L+ ++ + S+ END +L+K +W+G + ++I S+SDWPTNQI GCVHR +N + F K+G+NF+++C + +E IC+ S+CRN T + IL C L++++T HC+P + +N +SY PI + + +KL WIP+LSISQCL+ I N+SII IY+ +I+L K + K+ ++++S DI+ + I F I++S+G FI+ + + + + + IGWN N C P+ +KF IQ N N+KS Sbjct: 11 LVSILGISSSYLIGEIEGEFRNDLKIGIGIEFSQISDKYLLQAIDSNWNKLSIKTSKSFANDNQIIINFNGQTCTRTAYEDLDMIF-----------RYVAVFYLYVRLTFKANNLYASIWPSDELINIKLIPKYPKLIISNISYRSISRAAIENDWPSAGDLSKLTNRIKIWIGFDHDWIGKSHSDWPTNQIFGCVHRIDSIDVNRIDVNWNSVIFDKHGNNFYSDCYNKVTESICEQSQCRNETYKYQFILGCRLSRMSTAPHCYPDVTDNTEGLHSYQPITDGNVTLKLGWIPQLSISQCLMHIITSNESIIDIYINSANQIILETMIKKSIK-SKYNSYQISSVDIHALSIGFIDHNLTIRISNGSFIKTRMLPNQDRIKTVYIGWNPNRCLCHPIRNKFGIQLNSNLKSA 413
BLAST of SMED30006223 vs. Planmine SMEST
Match: SMESG000024557.1 (SMESG000024557.1) HSP 1 Score: 248.44 bits (633), Expect = 7.837e-76 Identity = 143/396 (36.11%), Postives = 225/396 (56.82%), Query Frame = 1 Query: 4 IIQLIGKFSCGLIGEFKYKFENHLKFEIFIDFLPIADKNLVQLIDSDQNRLLIQTSRSFAHDNKLIINFNGFTYNWISYQSFSLFYYKCC*FLTPDRRYQDIFFLDIKMKLINNKLYKLGSSSYVLLFPDVF--NGNGSKRMTEND------SIRQRNLAKVWLGHERNFINYSNSDWPTNQIIGCVHRFRLNNKKINFAKYGSNFFANCILQQSERICQDSECRNGTSFLHILDCSLAKVNTGRHCFPGLYNNVNKFYSYAPIINEPIRIKLAWIPKLSISQCLLEIGAKNDSIISIYVGPTGKIVLNIKHNKLTEVPKFILHRMTSNDIYVILIKFTVRKWEIQVSDGIFIRAQEVLDLQGVHFINIGWNTNVNFCQPLNDKFEIQHNINVKSG 1167 ++ ++G S LIGE + +F N L I I F I+DK L+Q IDS++N+L I+TS+SFA+D+KLIINFNG T +Y+ + + RY +L I + N +Y Y L+ ++ + N S+ END I+ N K+W+G++ ++I+ S+SDWPTNQIIGCV+R +N + +E IC+ S+CRN T + HILDC +++++T +C+P + +N +SY PI + + +KL WIP+LSISQCL+ I N+SII IY+ +I+L + K + K+ ++++S DI+ + I F I++S+G FI+ + + + + I IGWN N C P+ +KF IQ N N+KS Sbjct: 11 LVSILGISSSYLIGEIEGEFRNDLIIVITIRFSQISDKYLLQPIDSNRNKLSIKTSKSFANDSKLIINFNGQTCTRTAYEDLDMIF-----------RYMGALYLYIVLAFKANNVYAGKWPPYELIISNISYRSSNSSRAAIENDWPSAGDLIKLTNRIKIWIGYDHDWISKSHSDWPTNQIIGCVYRIHVNGNSV-----------------TESICEQSQCRNETDYQHILDCRISRMSTAPNCYPDVTDNTEGLHSYQPITDGHVTLKLGWIPQLSISQCLIHIITSNESIIDIYINSANQIILEMTIKKSIK-SKYNSYQISSVDIHALSIGFIDHNLTIRISNGSFIKTRMLPNQDRIKTIYIGWNPNPCLCYPIRNKFGIQLNSNLKSA 377 The following BLAST results are available for this feature:
BLAST of SMED30006223 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006223 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006223 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006223 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006223 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006223 ID=SMED30006223|Name=SMED30006223|organism=Schmidtea mediterranea sexual|type=transcript|length=1495bpback to top protein sequence of SMED30006223-orf-1 >SMED30006223-orf-1 ID=SMED30006223-orf-1|Name=SMED30006223-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=359bp MKLINNKLYKLGSSSYVLLFPDVFNGNGSKRMTENDSIRQRNLAKVWLGHback to top Annotated Terms
The following terms have been associated with this transcript:
|