Si:ch211-215k15.4

Overview


Name	Si:ch211-215k15.4
Smed ID	SMED30006168
Length (bp)	886

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Si:ch211-215k15.4 (SMED30006168) t-SNE clustered cells

Violin plots show distribution of expression levels for Si:ch211-215k15.4 (SMED30006168) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Si:ch211-215k15.4 (SMED30006168) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Si:ch211-215k15.4 (SMED30006168) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30006168

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 8

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30006168	h1SMcG0005582	SmedASXL_070356	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
nervous system	SMED30006168	h1SMcG0005582	dd_Smed_v4_15258_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30006168	h1SMcG0005582	dd_Smed_v4_15258_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30006168	h1SMcG0005581	Contig33473	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30006168	h1SMcG0005581	Contig33473	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30006168	h1SMcG0005582	Contig33473	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30006168	h1SMcG0005582	Contig33473	newmark_ests	PMID:29674431 Fincher et al., 2018
zeta neoblast	SMED30006168	h1SMcG0005582	dd_Smed_v4_15258_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Si:ch211-215k15.4 vs. Ensembl Zebrafish
Match: si:ch211-215k15.4 (si:ch211-215k15.4 [Source:ZFIN;Acc:ZDB-GENE-131127-215])

HSP 1 Score: 61.6178 bits (148), Expect = 1.844e-10
Identity = 42/129 (32.56%), Postives = 63/129 (48.84%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLV-ARTVMVAQKISSTSIELCEFEVYGHP 577
            +S A+DGN+    Y+G C ++    D WW + L D YVV  ITI  R DCC  RL     +     ++   S   +          PA G S ++F + + +  R + V  + S+  + LCE EVYG+P
Sbjct:   50 ASNAIDGNRDPDFYHGSCTATEVQDDPWWRLDLLDTYVVKSITITNRKDCCPERLDGAEVHIGNSLLNNGNSNPLA----AKISSIPA-GRS-LTFKWKKGISGRYINVILRGSNRILTLCELEVYGYP 172

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BLAST of Si:ch211-215k15.4 vs. Ensembl Zebrafish
Match: si:ch211-215k15.4 (si:ch211-215k15.4 [Source:ZFIN;Acc:ZDB-GENE-131127-215])

HSP 1 Score: 61.2326 bits (147), Expect = 2.206e-10
Identity = 42/129 (32.56%), Postives = 63/129 (48.84%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLV-ARTVMVAQKISSTSIELCEFEVYGHP 577
            +S A+DGN+    Y+G C ++    D WW + L D YVV  ITI  R DCC  RL     +     ++   S   +          PA G S ++F + + +  R + V  + S+  + LCE EVYG+P
Sbjct:   45 ASNAIDGNRDPDFYHGSCTATEVQDDPWWRLDLLDTYVVKSITITNRKDCCPERLDGAEVHIGNSLLNNGNSNPLA----AKISSIPA-GRS-LTFKWKKGISGRYINVILRGSNRILTLCELEVYGYP 167

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BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
Match: ptx (pentraxin [Source:NCBI gene;Acc:100127855])

HSP 1 Score: 51.6026 bits (122), Expect = 9.516e-7
Identity = 24/68 (35.29%), Postives = 37/68 (54.41%), Query Frame = 2

Query:  170 ASQNVTWD----SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            +SQ+ T+D    +  A+DG+      +GHC  +    + WW V L D Y VT + I  R DCC +R++
Sbjct:  400 SSQSSTYDHFGNAENAIDGSSSTRYMSGHCSHTDLDIEPWWRVDLTDTYNVTMVKITNRGDCCNDRIN 467

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BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
Match: ncapg2 (non-SMC condensin II complex subunit G2 [Source:Xenbase;Acc:XB-GENE-1002744])

HSP 1 Score: 49.6766 bits (117), Expect = 3.409e-6
Identity = 36/126 (28.57%), Postives = 53/126 (42.06%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYG 571
            +S A DGNK  ++  G C  + S    WW + L   Y V ++ I  R DCC  RL          + ++     +     Q        GT  +S N   +  R V V     + +++LCE EVYG
Sbjct:  189 ASNANDGNKNANMMKGSCSLTGSDNPAWWQLDLKKPYSVEKVVIVNRGDCCWERLQGAEVRVGSSTNNKNPVCGTVTDTSQ--------GTITLSCNG--MEGRYVSVVIPRRAETLQLCEVEVYG 304

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Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYG 571
            +S A DGNK  ++  G C  + S    WW + L   Y V ++ I  R DCC  RL          + ++     +     Q        GT  +S N   +  R V V     + +++LCE EVYG
Sbjct:  189 ASNANDGNKNANMMKGSCSLTGSDNPAWWQLDLKKPYSVEKVVIVNRGDCCWERLQGAEVRVGSSTNNKNPVCGTVTDTSQ--------GTITLSCNG--MEGRYVSVVIPRRAETLQLCEVEVYG 304

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
Match: ncapg2 (non-SMC condensin II complex subunit G2 [Source:Xenbase;Acc:XB-GENE-1002744])

HSP 1 Score: 49.2914 bits (116), Expect = 3.734e-6
Identity = 36/126 (28.57%), Postives = 53/126 (42.06%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYG 571
            +S A DGNK  ++  G C  + S    WW + L   Y V ++ I  R DCC  RL          + ++     +     Q        GT  +S N   +  R V V     + +++LCE EVYG
Sbjct:  190 ASNANDGNKNANMMKGSCSLTGSDNPAWWQLDLKKPYSVEKVVIVNRGDCCWERLQGAEVRVGSSTNNKNPVCGTVTDTSQ--------GTITLSCNG--MEGRYVSVVIPRRAETLQLCEVEVYG 305

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
Match: ncapg2 (non-SMC condensin II complex subunit G2 [Source:Xenbase;Acc:XB-GENE-1002744])

HSP 1 Score: 49.2914 bits (116), Expect = 4.007e-6
Identity = 36/126 (28.57%), Postives = 53/126 (42.06%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYG 571
            +S A DGNK  ++  G C  + S    WW + L   Y V ++ I  R DCC  RL          + ++     +     Q        GT  +S N   +  R V V     + +++LCE EVYG
Sbjct:  189 ASNANDGNKNANMMKGSCSLTGSDNPAWWQLDLKKPYSVEKVVIVNRGDCCWERLQGAEVRVGSSTNNKNPVCGTVTDTSQ--------GTITLSCNG--MEGRYVSVVIPRRAETLQLCEVEVYG 304

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BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Match: sp|Q7SIC1|FUCL_ANGAN (Fucolectin OS=Anguilla anguilla OX=7936 PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.468e-8
Identity = 23/57 (40.35%), Postives = 33/57 (57.89%), Query Frame = 2

Query:  191 DSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            ++S A+DGN+  + Y+G C  S    + WW V L   Y +T +TI  R DCC  R+S
Sbjct:   32 EASNAIDGNRDSNFYHGSCTHSSGQANPWWRVDLLQVYTITSVTITNRGDCCGERIS 88

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BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Match: sp|Q9I926|FUCL6_ANGJA (Fucolectin-6 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 2.806e-6
Identity = 23/62 (37.10%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  188 WDS----SRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            WD+    S A+DGN+    Y+G C  +    + WW V L   Y +T +TI  R DCC  R++
Sbjct:   59 WDAFSHASNAIDGNQDSYFYHGSCTHTSGGANPWWRVDLLQEYKITSVTITNRGDCCGERIT 120

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BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Match: sp|Q9I929|FUCL3_ANGJA (Fucolectin-3 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 3.021e-6
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            +S A+DGN+  + ++G C S     + WW V L   Y +T +TI  R DCC  R+S
Sbjct:   56 ASNAIDGNRDSNYHHGSC-SHTEGDNSWWRVDLKQVYTITSVTITNRGDCCGERIS 110

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BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Match: sp|Q9I931|FUCL1_ANGJA (Fucolectin-1 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 3.992e-6
Identity = 21/53 (39.62%), Postives = 30/53 (56.60%), Query Frame = 2

Query:  203 AVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            A+DGN+     +G C  + +S + WW V L   Y +T +TI  R DCC  R+S
Sbjct:   57 AIDGNRDSDFSHGSCSHTTNSPNPWWRVDLLQLYTITSVTITNRGDCCGERIS 109

[Close]

BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Match: sp|Q9I925|FUCL7_ANGJA (Fucolectin-7 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 4.642e-6
Identity = 23/57 (40.35%), Postives = 34/57 (59.65%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDR-WWLVVLDDAYVVTQITIYARTDCCTNRLS 361
            +S A+DGN+    Y+G C  +++  D+ WW V L   Y +T +TI  R DCC  R+S
Sbjct:   65 ASNAIDGNRDSVFYHGSC--THTEADKPWWRVDLLQVYTITSVTITNRGDCCEERIS 119

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BLAST of Si:ch211-215k15.4 vs. TrEMBL
Match: A0A3P8NSC3 (Si:ch211-215k15.4 OS=Astatotilapia calliptera OX=8154 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.398e-8
Identity = 50/172 (29.07%), Postives = 72/172 (41.86%), Query Frame = 2

Query:  203 AVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFT-RLVARTVMVAQKISSTSIELCEFEVYG-HPFYQPNPIIYTSKPALYATAIAIRAFQTMSIV---------KCSQV 685
            A+DGN+   + +G C  +    D WW V L ++Y+VT ITI  R DCC   L+ L  Y      +   +        +   G         + NF  R+  R V +    S   + LCE EVYG H     N   Y SK A YA  +     Q+  ++         KC++V
Sbjct:   36 AIDGNRDSDLRHGSCSHTDQQTDPWWRVDLLESYIVTSITITNRGDCCHEELNGLEIYIGNSLNNDGLNNPKVGKISEVGAGKS------YTVNFAGRVEGRYVTLTLPGSERILALCEVEVYGYHAPTGENLNYYMSKCARYAAILPNSGIQSFKLILKQFNNRYMKCNKV 201

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BLAST of Si:ch211-215k15.4 vs. TrEMBL
Match: A0A1S3I1T9 (fucolectin-like OS=Lingula unguis OX=7574 GN=LOC106160239 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.736e-8
Identity = 58/218 (26.61%), Postives = 95/218 (43.58%), Query Frame = 2

Query:  128 SQNKFTNSSPAYY---YASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQY---FQGPPALGTSVVSFNFTRLVARTVMVAQKISSTS-IELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQVCRETVNCVSFTLISNGT----CEIF 748
            +QN      PAY    Y + N    +SR+VDGN    + NG C  +     +WWLV L+    V  + I  R DCC+ RL+ +        +      +SSF +++    + G   +G + +S   + L  R V++ +  S  S ++LCE EV+G                  AT + I      S+++CS  C +   CV+  ++  G     CE+ 
Sbjct:   25 TQN-LAKGKPAYQSTTYVNVNGVHPASRSVDGNYDNVMSNGRCSHTDLGQGQWWLVDLETTASVAGVIITNRGDCCSERLNNVE-----VRIGTSYVNNSSFTQYKLCASYPGVAPVGNTSMSCG-SPLRGRYVLIIKVGSDDSYLQLCEVEVHG---ITSTTTFKRVAADARATLVPISTLNVRSLIECSISCVQNWKCVAINILCAGNDQCVCELL 232

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BLAST of Si:ch211-215k15.4 vs. TrEMBL
Match: Q4T7L0 (Chromosome undetermined SCAF8088, whole genome shotgun sequence OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00005680001 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.083e-8
Identity = 43/139 (30.94%), Postives = 64/139 (46.04%), Query Frame = 2

Query:  176 QNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVV----SFNFTRLV-ARTVMVAQKISSTSIELCEFEVYGHP 577
            ++V  D+  A+DGN+ G+  +G C  +    + WW V L D Y +T ITI  R DCC  RLS +  +                      Q P A   + V    + NFT+ V  R V ++    +  + LCE EV+G+P
Sbjct:   40 EHVFGDAHSAIDGNRDGTFNHGSCTHTAEQREPWWTVDLLDPYAITSITITNRQDCCPFRLSGVRIH---------------IGNNNGLQNPVATSIASVGKEYTHNFTKPVEGRYVTLSLPGRTRILSLCEVEVFGYP 163

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BLAST of Si:ch211-215k15.4 vs. TrEMBL
Match: A0A498MNV1 (Uncharacterized protein OS=Labeo rohita OX=84645 GN=ROHU_025076 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.086e-8
Identity = 48/137 (35.04%), Postives = 65/137 (47.45%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTR-LVARTVMVAQKISSTSIELCEFEVYGHP-FY-QPNP 595
            +S A+DGN     ++G C  + S  D WW + L D YVVT ITI  R DCC  RL     +      +   S   +          PA G+  V+F+  + +  R V V    S+  + LCE EVYG+P FY  P P
Sbjct:  404 ASNAIDGNLDSHFHHGSCTHTDSQNDPWWRLDLLDEYVVTSITITNRKDCCPERLHGAEIHIGNSLQNNGNSNPLA----GKISSVPAGGS--VTFDLKKGIPGRYVNVVIPGSNRVLTLCEVEVYGYPAFYGSPGP 534

HSP 2 Score: 59.6918 bits (143), Expect = 3.310e-6
Identity = 40/129 (31.01%), Postives = 58/129 (44.96%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLV-ARTVMVAQKISSTSIELCEFEVYGHP 577
            +S A+DGN     ++G C  + S  D WW + L D YVVT I I  R DCC  RL     +     ++   S   +        G        V+F+  + +  R + V    S+  + LCE EVYG+P
Sbjct:   50 ASNAIDGNLDSHFHHGSCTHTDSQNDPWWRLDLLDEYVVTSIIITNRKDCCPERLDGAEIHIGNSLLNNGNSNPLAGKISSIPDG------GSVTFDLKKGISGRYINVVIPGSNRVLTLCEVEVYGYP 172

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BLAST of Si:ch211-215k15.4 vs. TrEMBL
Match: A0A0F8AS36 (Fucolectin-6 (Fragment) OS=Larimichthys crocea OX=215358 GN=EH28_00052 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.701e-8
Identity = 46/163 (28.22%), Postives = 72/163 (44.17%), Query Frame = 2

Query:  203 AVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGP------PALGTSVVSFNFTRLV-ARTVMVAQKISSTSIELCEFEVYGHPFYQP---NPIIYTSKPALYATAIAIRAFQTM 661
            A+DGN+  + ++G C  +    + WW V L D+Y++T ITIY R DCC  R++ L  +                 E      P         G+   +  FT  V  R V ++   S+  + LCE EVYG  +  P   N   Y +K   YA ++ +   +T+
Sbjct:   33 AIDGNRDSNFHDGSCTHTSGKPNPWWRVDLLDSYIITSITIYNRGDCCQERINGLRIHIGNSL------------EHNGLNNPLVGQIVDLHGSPTFTQTFTPHVKGRYVTLSLPGSNKYLTLCEVEVYG--YRAPTGENLSFYINKCEHYAESLTLSKGKTL 181

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BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Match: ENSAMXT00000035078.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:30955763:30962150:-1 gene:ENSAMXG00000041548.1 transcript:ENSAMXT00000035078.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 5.006e-9
Identity = 46/187 (24.60%), Postives = 76/187 (40.64%), Query Frame = 2

Query:  128 SQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVV-SFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQV 685
            S+      S A   ++ N   D+  AVDGN   +   G C  +    D W  V L   Y +T +TI+ R DCC+ R+     + +   +      + +  +     GP  +G+ V+ ++ F     R V +        + LCE +VY    + P       K  L    IA+R   T S +  S++
Sbjct:   20 SKENIAVRSRAVQSSTYNELADAEHAVDGNIDSNYMRGSCTHTMPETDPWLRVELPGVYKITSVTIFNRGDCCSERI-----HGAQVRIGNSLKNNGNENKIVAVIGP--MGSEVIKTYKFKATEGRFVNIFLPGQRKILTLCEVKVYADEIFDPKSFYLHDKEDL--DNIALRGTATQSSLHSSEI 197

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Match: ENSAMXT00000041629.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:30955763:30962150:-1 gene:ENSAMXG00000041548.1 transcript:ENSAMXT00000041629.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.225 bits (134), Expect = 1.154e-8
Identity = 43/166 (25.90%), Postives = 70/166 (42.17%), Query Frame = 2

Query:  191 DSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVV-SFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQV 685
            D++ AVDGN   +     C  ++S  + W  V L D Y VT +TI  R DCC +R++      +   +        +  +     GP  +G+ V+ ++ F     R V +        + LCE +VY    + P       K  L    IA+R   T S +  S++
Sbjct:   41 DAANAVDGNSNTNYLKKSCSHTHSDNNPWLRVELPDVYNVTSVTIVNRKDCCGDRIN-----GAQIRIGNSLDNKGNDNKLVAVIGP--MGSEVIKTYKFKATEGRFVNIFLPGQRKILTLCEVKVYADEIFDPKSFYLHDKEDL--DNIALRGTATQSSLHSSEI 197

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Match: ENSAMXT00000052178.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:30955763:30962678:-1 gene:ENSAMXG00000041548.1 transcript:ENSAMXT00000052178.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.225 bits (134), Expect = 1.154e-8
Identity = 43/166 (25.90%), Postives = 70/166 (42.17%), Query Frame = 2

Query:  191 DSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVV-SFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQV 685
            D++ AVDGN   +     C  ++S  + W  V L D Y VT +TI  R DCC +R++      +   +        +  +     GP  +G+ V+ ++ F     R V +        + LCE +VY    + P       K  L    IA+R   T S +  S++
Sbjct:   41 DAANAVDGNSNTNYLKKSCSHTHSDNNPWLRVELPDVYNVTSVTIVNRKDCCGDRIN-----GAQIRIGNSLDNKGNDNKLVAVIGP--MGSEVIKTYKFKATEGRFVNIFLPGQRKILTLCEVKVYADEIFDPKSFYLHDKEDL--DNIALRGTATQSSLHSSEI 197

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Match: ENSAMXT00000048793.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000995.1:82123:94892:-1 gene:ENSAMXG00000040615.1 transcript:ENSAMXT00000048793.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 3.058e-8
Identity = 49/181 (27.07%), Postives = 75/181 (41.44%), Query Frame = 2

Query:  125 LSQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTR-LVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKP---ALYATAIAIRAFQ 655
            L QN +T    AY             A+DGN   +  +G C ++    + WW V L D Y +T ITI  R DCC  R++     A     + + +  ++          PA  +   +FNF R L  R V V     +  + LCE EVYG+P  + + +    +    +LY     + A  
Sbjct:   40 LVQNPWTGHGHAY------------NAIDGNTDSNYDHGSCTATTFQPNPWWRVDLLDEYTITSITITNRGDCCAERING----AQIRIGNSLVNNGNNNPWAGVISTIPAGKSE--TFNFQRGLSGRYVNVVIPGDNRLLTLCEVEVYGYPTPEGDNVALKGRATQSSLYGNGFPLTAID 202

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Match: ENSAMXT00000049705.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000995.1:82123:94892:-1 gene:ENSAMXG00000040615.1 transcript:ENSAMXT00000049705.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 3.058e-8
Identity = 49/181 (27.07%), Postives = 75/181 (41.44%), Query Frame = 2

Query:  125 LSQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTR-LVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKP---ALYATAIAIRAFQ 655
            L QN +T    AY             A+DGN   +  +G C ++    + WW V L D Y +T ITI  R DCC  R++     A     + + +  ++          PA  +   +FNF R L  R V V     +  + LCE EVYG+P  + + +    +    +LY     + A  
Sbjct:   40 LVQNPWTGHGHAY------------NAIDGNTDSNYDHGSCTATTFQPNPWWRVDLLDEYTITSITITNRGDCCAERING----AQIRIGNSLVNNGNNNPWAGVISTIPAGKSE--TFNFQRGLSGRYVNVVIPGDNRLLTLCEVEVYGYPTPEGDNVALKGRATQSSLYGNGFPLTAID 202

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Match: EDO42275 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1W6])

HSP 1 Score: 50.0618 bits (118), Expect = 6.566e-8
Identity = 40/131 (30.53%), Postives = 55/131 (41.98%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQ 586
            S  AVDG + G+  +  C S+    + WW V L  +YV+ +  I  R DCC  RL           V+      S    +   QG    G S+     T L  R V +  +    S+ LCE EVY  PF +
Sbjct:    1 SKHAVDGIRSGNYKDKSCASTARQTNPWWRVDLQKSYVILRFFIVNRADCCGERLDNFEIRIGDSLVNNGNDNPSCGSSYIIPQG---RGKSIAC--HTLLKGRYVNIRIQGPDRSVTLCEVEVY--PFIE 124

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Match: EDO40750 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6H5])

HSP 1 Score: 49.2914 bits (116), Expect = 2.026e-7
Identity = 38/131 (29.01%), Postives = 57/131 (43.51%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQ 586
            S+ AVDGN+ G+ Y   C S+ +  + WW V L  ++V  ++ +  R DCC  RL           V+      S    +   QG    G ++     T L  R V +  +     + LCE EVY  PF +
Sbjct:   24 SNHAVDGNRSGNYYQKSCSSTTAQTNPWWRVDLQKSHVNLRLFVVNRADCCGERLDNFEIRIGDSLVNDGNDNPSCGSSYIIPQG---RGNTIAC--HTLLKGRYVNIWIQGPDKVLTLCEVEVY--PFIE 147

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Match: EDO34462 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPB1])

HSP 1 Score: 48.521 bits (114), Expect = 2.282e-7
Identity = 34/130 (26.15%), Postives = 51/130 (39.23%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFN-----FTRLVARTVMVAQKISSTSIELCEFEVY 568
            +  AVDGN+ G  ++  C  +    + WW V L  +YV+ ++ I  R DCC +RL                       E      P   G+ ++  N      T L  R V +     +  + LCE EVY
Sbjct:   11 AEHAVDGNRNGRYFDLSCTETKVQSNPWWRVDLQKSYVILRLFIVNRADCCNHRLDNFE------------IRIGDSLENNGNDNPTCGGSYIIPKNGSIACHTLLKGRYVNIWIPGPNRLLTLCEVEVY 128

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Match: EDO29994 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T225])

HSP 1 Score: 48.9062 bits (115), Expect = 7.647e-7
Identity = 21/54 (38.89%), Postives = 33/54 (61.11%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNR 355
            +S AVDGN+ G+  N  C  + +  + WW V L  ++ V Q+ I AR+DCC ++
Sbjct:  158 ASHAVDGNRDGNYGNWSCTHTNAETNPWWRVDLGASHRVAQVFIVARSDCCGDK 211

[Close]

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Match: EDO35040 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMM0])

HSP 1 Score: 48.1358 bits (113), Expect = 1.158e-6
Identity = 31/107 (28.97%), Postives = 50/107 (46.73%), Query Frame = 2

Query:   68 FLNFLFAFGIGLVASADILLSQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNG----HCFSSYS--SYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLS 370
             L  +FAF + L+ +  + ++        P      +  T     A DG+K   + +      CF+S S  ++  WW V L   YVV ++ + +RTDCC NRL+ L 
Sbjct:    4 LLKEIFAFMMILLGTKILTINGGNIALRQPTASLNIREGTALPGLANDGDKDSKLTSPDNSFRCFASVSDPTHGGWWRVDLGRTYVVDEVFLISRTDCCPNRLAGLE 110

[Close]

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Match: SMESG000024841.1 (SMESG000024841.1)

HSP 1 Score: 339.732 bits (870), Expect = 5.057e-119
Identity = 176/182 (96.70%), Postives = 179/182 (98.35%), Query Frame = 2

Query:  236 NGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQVCRETVNCVSFTLISNGTCEIFSHAGGVYSMIP 781
             GHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLS YASYDSVSQ+TSGSSSFYEFQYFQGPPA GTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMS+VKCSQVCRETVNCVSFTLISNGTCEIFSHAGG+YSMIP
Sbjct:    3 EGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSLYASYDSVSQMTSGSSSFYEFQYFQGPPASGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSVVKCSQVCRETVNCVSFTLISNGTCEIFSHAGGIYSMIP 184

[Close]

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Match: SMESG000011037.1 (SMESG000011037.1)

HSP 1 Score: 296.59 bits (758), Expect = 9.542e-102
Identity = 164/241 (68.05%), Postives = 180/241 (74.69%), Query Frame = 2

Query:   62 MNFLNFLFAFGIGLVASADILLSQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTMSIVKCSQVCRETVNCVSFTLISNGTCEIFSHAGGVYSMIPE 784
            MN L FLFAFGIG  +SAD+LLS NKFTNSS AYY+AS N TWDSS+AVDGN  G IYNGHCFSSYSS D+WWLVVLD  YVVT+I IYARTDCCT+RLSTLS YASYDS+SQVTSGSSSFYEFQY+QGPPA G SVVSFNFTRL+  T+                                 KP+LY  A AI   +TMS+VKCS++C ETVNCVSFTL SNGTCEIFSHAGGVYSMIPE
Sbjct:    1 MNLLKFLFAFGIGFTSSADLLLSGNKFTNSSSAYYFASTNTTWDSSKAVDGNMDGVIYNGHCFSSYSSNDKWWLVVLDGVYVVTRIIIYARTDCCTSRLSTLSLYASYDSISQVTSGSSSFYEFQYYQGPPAPGISVVSFNFTRLLIDTL---------------------------------KPSLYVNASAILTTKTMSVVKCSKICLETVNCVSFTLTSNGTCEIFSHAGGVYSMIPE 208

[Close]

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Match: SMESG000011037.1 (SMESG000011037.1)

HSP 1 Score: 217.238 bits (552), Expect = 2.606e-71
Identity = 119/146 (81.51%), Postives = 128/146 (87.67%), Query Frame = 2

Query:   62 MNFLNFLFAFGIGLVASADILLSQNKFTNSSPAYYYASQNVTWDSSRAVDGNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLV 499
            MN L FLFAFGIG  +SAD+LLS NKFTNSS AYY+AS N TWDSS+AVDGN  G IYNGHCFSSYSS D+WWLVVLD  YVVT+I IYARTDCCT+RLSTLS YASYDS+SQVTSGSSSFYEFQY+QGPPA G SVVSFNFTRLV
Sbjct:    1 MNLLKFLFAFGIGFTSSADLLLSGNKFTNSSSAYYFASTNTTWDSSKAVDGNMDGVIYNGHCFSSYSSNDKWWLVVLDGVYVVTRIIIYARTDCCTSRLSTLSLYASYDSISQVTSGSSSFYEFQYYQGPPAPGISVVSFNFTRLV 146

[Close]

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Match: SMESG000024496.1 (SMESG000024496.1)

HSP 1 Score: 166.007 bits (419), Expect = 5.492e-50
Identity = 90/222 (40.54%), Postives = 135/222 (60.81%), Query Frame = 2

Query:  113 ADILLSQNKFTNSSPAYYYASQN--VTWDSSRAVDGNKGGSIYNGHCFSSYSS-YDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIYT--SKPALYATAIAIRAFQTMSIVKCSQVCRETVNCVSFTLISNGTCEIFSHAGG 763
            ++ILLS+N+ TNSS  Y     +   +   S AVDGN  G I    C+ S    +++WWLV LDD  V+T++ I++RTDCC NR S LS Y SY++ +  ++ SS FY+  +F GPP+     V+    R+V RT+++ + I   +I  CE EVYG+P+Y+ NP IY       LY+   AI+     S ++C++VC+E  +CVSFT  +N  C +++HAGG
Sbjct:   17 SEILLSKNRITNSSNVYRTDEGDGIASMVPSNAVDGNTNGDIKQNSCYHSKGEMFEKWWLVCLDDVSVITRVVIHSRTDCCYNRTSFLSIYTSYENETNFSTFSSLFYQINFFPGPPSGDKLAVAILLRRVVGRTIVIFKPIKEEAINFCEVEVYGYPYYRSNPSIYKLLGTYPLYSNIPAIQYRTCRSSIECARVCKELESCVSFTFDNNRLCNVYNHAGG 238

[Close]

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Match: SMESG000071684.1 (SMESG000071684.1)

HSP 1 Score: 146.362 bits (368), Expect = 5.306e-43
Identity = 76/199 (38.19%), Postives = 124/199 (62.31%), Query Frame = 2

Query:  194 SSRAVDGNKGGSIYNGHCFSSYS-SYDRWWLVVLDDAYVVTQITIYARTDCCTNRLSTLSSYAXXXXXXXXXXXXXXFYEFQYFQGPPALGTSVVSFNFTRLVARTVMVAQKISSTSIELCEFEVYGHPFYQPNPIIY----TSKPALYATAIAIRAFQTMSIVKCSQVCRETVNCVSFTLISNGTCEIFSHAGGVYSM 775
            S +AVDG     +    C+ S   + D+WWLV LD   V+T+I IY RTD C++R+S L+ +AS+DS    +S  ++FY+  Y+QGPP +   ++  NFTRLVA+TV + + +   SI++CEF+V+G+ + +  P IY    T +P    +  +I+ F+  S + C+++CRE  NCVSF++  +  C  ++H GG +S+
Sbjct:    3 SDKAVDGVYKTDVNQYSCYHSLEENKDKWWLVELDVVSVITRIVIYWRTDDCSDRMSNLTIFASFDSQLNYSSLLTNFYQIGYYQGPPLMQNFIIELNFTRLVAKTVGITKPLPDQSIDMCEFDVFGYSYVKYEPYIYKNYKTGEPEF--SIYSIQQFKARSNIHCAKLCREIQNCVSFSVNKDLICSFYNHGGGQFSI 199

The following BLAST results are available for this feature:

BLAST of Si:ch211-215k15.4 vs. Ensembl Human
BLAST of Si:ch211-215k15.4 vs. Ensembl Celegans
BLAST of Si:ch211-215k15.4 vs. Ensembl Fly
BLAST of Si:ch211-215k15.4 vs. Ensembl Zebrafish
BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
BLAST of Si:ch211-215k15.4 vs. Ensembl Mouse
BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
BLAST of Si:ch211-215k15.4 vs. TrEMBL
BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
BLAST of Si:ch211-215k15.4 vs. Ensembl Sea Lamprey
BLAST of Si:ch211-215k15.4 vs. Ensembl Yeast
BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
BLAST of Si:ch211-215k15.4 vs. Ensembl Medaka
BLAST of Si:ch211-215k15.4 vs. Planmine SMEST

BLAST of Si:ch211-215k15.4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
si:ch211-215k15.4	1.844e-10	32.56	si:ch211-215k15.4 [Source:ZFIN;Acc:ZDB-GENE-131127...	[more]
si:ch211-215k15.4	2.206e-10	32.56	si:ch211-215k15.4 [Source:ZFIN;Acc:ZDB-GENE-131127...	[more]

BLAST of Si:ch211-215k15.4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ptx	9.516e-7	35.29	pentraxin [Source:NCBI gene;Acc:100127855]	[more]
ncapg2	3.409e-6	28.57	non-SMC condensin II complex subunit G2 [Source:Xe...	[more]
ncapg2	3.409e-6	28.57	non-SMC condensin II complex subunit G2 [Source:Xe...	[more]
ncapg2	3.734e-6	28.57	non-SMC condensin II complex subunit G2 [Source:Xe...	[more]
ncapg2	4.007e-6	28.57	non-SMC condensin II complex subunit G2 [Source:Xe...	[more]

BLAST of Si:ch211-215k15.4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q7SIC1\|FUCL_ANGAN	5.468e-8	40.35	Fucolectin OS=Anguilla anguilla OX=7936 PE=1 SV=1	[more]
sp\|Q9I926\|FUCL6_ANGJA	2.806e-6	37.10	Fucolectin-6 OS=Anguilla japonica OX=7937 PE=2 SV=...	[more]
sp\|Q9I929\|FUCL3_ANGJA	3.021e-6	39.29	Fucolectin-3 OS=Anguilla japonica OX=7937 PE=2 SV=...	[more]
sp\|Q9I931\|FUCL1_ANGJA	3.992e-6	39.62	Fucolectin-1 OS=Anguilla japonica OX=7937 PE=2 SV=...	[more]
sp\|Q9I925\|FUCL7_ANGJA	4.642e-6	40.35	Fucolectin-7 OS=Anguilla japonica OX=7937 PE=2 SV=...	[more]

BLAST of Si:ch211-215k15.4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3P8NSC3	1.398e-8	29.07	Si:ch211-215k15.4 OS=Astatotilapia calliptera OX=8...	[more]
A0A1S3I1T9	2.736e-8	26.61	fucolectin-like OS=Lingula unguis OX=7574 GN=LOC10...	[more]
Q4T7L0	3.083e-8	30.94	Chromosome undetermined SCAF8088, whole genome sho...	[more]
A0A498MNV1	3.086e-8	35.04	Uncharacterized protein OS=Labeo rohita OX=84645 G...	[more]
A0A0F8AS36	3.701e-8	28.22	Fucolectin-6 (Fragment) OS=Larimichthys crocea OX=...	[more]

BLAST of Si:ch211-215k15.4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000035078.1	5.006e-9	24.60	pep primary_assembly:Astyanax_mexicanus-2.0:2:3095...	[more]
ENSAMXT00000041629.1	1.154e-8	25.90	pep primary_assembly:Astyanax_mexicanus-2.0:2:3095...	[more]
ENSAMXT00000052178.1	1.154e-8	25.90	pep primary_assembly:Astyanax_mexicanus-2.0:2:3095...	[more]
ENSAMXT00000048793.1	3.058e-8	27.07	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000049705.1	3.058e-8	27.07	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of Si:ch211-215k15.4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO42275	6.566e-8	30.53	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO40750	2.026e-7	29.01	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO34462	2.282e-7	26.15	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29994	7.647e-7	38.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35040	1.158e-6	28.97	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Si:ch211-215k15.4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Si:ch211-215k15.4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000024841.1	5.057e-119	96.70	SMESG000024841.1	[more]
SMESG000011037.1	9.542e-102	68.05	SMESG000011037.1	[more]
SMESG000011037.1	2.606e-71	81.51	SMESG000011037.1	[more]
SMESG000024496.1	5.492e-50	40.54	SMESG000024496.1	[more]
SMESG000071684.1	5.306e-43	38.19	SMESG000071684.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30006168 ID=SMED30006168|Name=Si:ch211-215k15.4|organism=Schmidtea mediterranea sexual|type=transcript|length=886bp
TCTCAATATTTATTCCAAATTTTTGTTTTTCTATTTTTGAATTTTTATTT
TACAATTTACGATGAATTTTTTGAATTTTCTTTTTGCTTTTGGTATTGGA
CTTGTTGCATCGGCAGATATCCTGTTATCGCAGAATAAATTCACGAACTC
GTCTCCAGCATACTATTACGCGTCGCAGAATGTCACTTGGGACTCGTCGA
GAGCTGTAGATGGAAATAAAGGGGGATCTATTTATAATGGTCATTGCTTT
TCAAGTTATTCATCTTATGACAGATGGTGGCTTGTCGTACTTGATGATGC
CTATGTCGTGACCCAGATCACAATTTACGCAAGAACGGACTGCTGTACAA
ACAGGTTGAGCACCTTGTCGTCATACGCCAGTTATGATAGTGTCAGTCAG
GTGACTTCAGGATCCTCTTCATTTTATGAATTCCAATACTTTCAAGGACC
TCCCGCTTTAGGGACATCGGTGGTGTCGTTTAATTTTACTAGATTGGTGG
CAAGGACAGTAATGGTCGCACAGAAAATATCGAGCACCAGCATAGAATTG
TGTGAATTTGAAGTATATGGACATCCGTTTTACCAACCGAATCCAATCAT
CTACACTTCTAAACCCGCTCTTTATGCCACTGCCATCGCTATTCGGGCTT
TCCAAACGATGTCGATTGTGAAATGCTCTCAAGTATGCCGAGAGACCGTC
AATTGTGTCTCCTTTACTTTGATTTCAAATGGAACATGTGAGATCTTCAG
CCATGCAGGAGGGGTGTATTCAATGATTCCGGAATGATAATTAAGCTATA
CTTTCAATAAAATGATTTATCTTTTTGTTCACTTTTTGATCTGATTTTGT
TTATTTCAATATTTTCTTTATTTTGCATTTTTATAC

protein sequence of SMED30006168-orf-1

>SMED30006168-orf-1 ID=SMED30006168-orf-1|Name=SMED30006168-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=242bp
MNFLNFLFAFGIGLVASADILLSQNKFTNSSPAYYYASQNVTWDSSRAVD
GNKGGSIYNGHCFSSYSSYDRWWLVVLDDAYVVTQITIYARTDCCTNRLS
TLSSYASYDSVSQVTSGSSSFYEFQYFQGPPALGTSVVSFNFTRLVARTV
MVAQKISSTSIELCEFEVYGHPFYQPNPIIYTSKPALYATAIAIRAFQTM
SIVKCSQVCRETVNCVSFTLISNGTCEIFSHAGGVYSMIPE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000421	FA58C
IPR008979	Galactose-bd-like_sf

Vocabulary: **biological process**
Term	Definition
GO:0001868	regulation of complement activation, lectin pathway
GO:0010185	regulation of cellular defense response
GO:0045088	regulation of innate immune response

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding
GO:0030246	carbohydrate binding
GO:0042806	fucose binding
GO:0046872	metal ion binding

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008979	Galactose-binding-like domain superfamily	GENE3D	G3DSA:2.60.120.260		coord: 21..172 e-value: 4.0E-26 score: 93.8
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	SSF49785	Galactose-binding domain-like	coord: 31..173
IPR000421	Coagulation factor 5/8 C-terminal domain	PFAM	PF00754	F5_F8_type_C	coord: 32..150 e-value: 7.7E-7 score: 29.3
None	No IPR available	PANTHER	PTHR45713	FAMILY NOT NAMED	coord: 23..206
None	No IPR available	PANTHER	PTHR45713:SF6	SI:CABZ01040626.2-RELATED	coord: 23..206
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..17 score: 0.825

Main menu

Si:ch211-215k15.4

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 8

Owlery

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