Ras-like protein 3

Overview
NameRas-like protein 3
Smed IDSMED30006167
Length (bp)1047
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ras-like protein 3 (SMED30006167) t-SNE clustered cells

Violin plots show distribution of expression levels for Ras-like protein 3 (SMED30006167) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ras-like protein 3 (SMED30006167) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ras-like protein 3 (SMED30006167) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30006167

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30006167h1SMcG0015611 Contig44333uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30006167h1SMcG0015611 Contig44333newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30006167h1SMcG0015611 dd_Smed_v4_7739_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30006167h1SMcG0015611 dd_Smed_v4_7739_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Ras-like protein 3 vs. Ensembl Human
Match: RAP1B (RAP1B, member of RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9857])

HSP 1 Score: 134.42 bits (337), Expect = 1.684e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Human
Match: RAP1B (RAP1B, member of RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9857])

HSP 1 Score: 134.42 bits (337), Expect = 1.684e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Human
Match: RAP1B (RAP1B, member of RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9857])

HSP 1 Score: 134.42 bits (337), Expect = 1.684e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Human
Match: RAP1B (RAP1B, member of RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9857])

HSP 1 Score: 134.42 bits (337), Expect = 1.684e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Human
Match: RAP1A (RAP1A, member of RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9855])

HSP 1 Score: 132.494 bits (332), Expect = 7.422e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Celegans
Match: rap-1 (Ras-related protein Rap-1 [Source:UniProtKB/Swiss-Prot;Acc:Q18246])

HSP 1 Score: 132.494 bits (332), Expect = 4.049e-37
Identity = 72/162 (44.44%), Postives = 100/162 (61.73%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YKI +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN +  L+D IL +K    VP+ILVGNK DL  E R V +   + +A ++   F E SAK  +NV E F DL+
Sbjct:    1 MREYKIVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLMDLRDQILRVKDTDEVPMILVGNKCDLEDE-RVVGKDQGQNLARQFGSAFLETSAKAKINVSEVFYDLV 161          
BLAST of Ras-like protein 3 vs. Ensembl Celegans
Match: rap-2 (RAP homolog (Vertebrate Rap GTPase family) [Source:UniProtKB/TrEMBL;Acc:O17599])

HSP 1 Score: 125.561 bits (314), Expect = 1.222e-34
Identity = 71/162 (43.83%), Postives = 103/162 (63.58%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M ++K+ +LG+ GVGKS L +QFV   F  + + TIED Y+K I V      +EI+DTAGTE  S  +D YI    G VVVYSIT + +F+ I+ +K+ I+ +K   NVP++LVGNK DL+ + RQV+      +A  W+C F E SAK++ NV   F +++
Sbjct:    1 MREFKVVVLGSGGVGKSALTVQFVSSTFIEKYDPTIEDFYRKEIEVDGQPSVLEILDTAGTEQFSSMRDLYIKNGQGFVVVYSITSQQTFHDIRNMKEQIVRVKGSENVPILLVGNKCDLSHQ-RQVRSEEGLALAESWSCPFTECSAKNNQNVNVTFAEIV 161          
BLAST of Ras-like protein 3 vs. Ensembl Celegans
Match: ral-1 (RAL (Ras-related GTPase) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N3F8])

HSP 1 Score: 114.39 bits (285), Expect = 5.603e-30
Identity = 63/167 (37.72%), Postives = 95/167 (56.89%), Query Frame = 1
Query:   73 PSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIK-QDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            P  + V +K+ ++G  GVGKS L +QF+ +EF    E T  D Y+K + +      I+I+DTAG E  S  +D Y    +G + V+SI D  SF      ++ IL +K  DS+VP++LVGNK D+ ++ R V     ++ A +W C + E SAK   NV + F DL+
Sbjct:   11 PPQQQVVHKVIMVGTGGVGKSALTLQFMYDEFVEEYEPTKADSYRKKVVLDGEECSIDILDTAGQEDYSAIRDNYYRSGEGFICVFSILDMESFEATNEFREQILRVKNSDSSVPIVLVGNKGDM-RDQRVVSAELCRQRAEQWGCHYVETSAKRRENVDKVFYDLM 176          
BLAST of Ras-like protein 3 vs. Ensembl Celegans
Match: ral-1 (RAL (Ras-related GTPase) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N3F8])

HSP 1 Score: 114.39 bits (285), Expect = 1.267e-29
Identity = 63/167 (37.72%), Postives = 95/167 (56.89%), Query Frame = 1
Query:   73 PSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIK-QDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            P  + V +K+ ++G  GVGKS L +QF+ +EF    E T  D Y+K + +      I+I+DTAG E  S  +D Y    +G + V+SI D  SF      ++ IL +K  DS+VP++LVGNK D+ ++ R V     ++ A +W C + E SAK   NV + F DL+
Sbjct:   52 PPQQQVVHKVIMVGTGGVGKSALTLQFMYDEFVEEYEPTKADSYRKKVVLDGEECSIDILDTAGQEDYSAIRDNYYRSGEGFICVFSILDMESFEATNEFREQILRVKNSDSSVPIVLVGNKGDM-RDQRVVSAELCRQRAEQWGCHYVETSAKRRENVDKVFYDLM 217          
BLAST of Ras-like protein 3 vs. Ensembl Celegans
Match: rap-3 (RAP homolog (Vertebrate Rap GTPase family) [Source:UniProtKB/TrEMBL;Acc:O01312])

HSP 1 Score: 108.227 bits (269), Expect = 7.936e-28
Identity = 65/158 (41.14%), Postives = 94/158 (59.49%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAF 558
            M +YKI +LG  GVGKS L +Q+V   F    ++TIED Y+K  +V     ++EI+DTAGTE  +  ++ Y   + G V+V+S+ + ++F  +K+    I+ I+    VP+ILVGNK DLA E RQV E  A   A K    + E SA+ + NV E F
Sbjct:    1 MKEYKIVVLGNGGVGKSALTLQYVQGIFVHTYDATIEDSYRKLSKVDAENARLEILDTAGTEQFTGMRETYYRTAQGFVLVFSLAETSTFENLKQTFTDIIAIR-GKEVPMILVGNKSDLA-ETRQVDESEAHNFAEKLKIPYFETSARLNQNVSEVF 156          
BLAST of Ras-like protein 3 vs. Ensembl Fly
Match: Rap1 (gene:FBgn0004636 transcript:FBtr0303154)

HSP 1 Score: 135.961 bits (341), Expect = 2.053e-38
Identity = 72/162 (44.44%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YKI +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN ++ L++ IL +K   +VP++LVGNK DL +E    KE+  K +A ++NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKEL-GKNLATQFNCAFMETSAKAKVNVNDIFYDLV 161          
BLAST of Ras-like protein 3 vs. Ensembl Fly
Match: Rap1 (gene:FBgn0004636 transcript:FBtr0072867)

HSP 1 Score: 135.961 bits (341), Expect = 2.053e-38
Identity = 72/162 (44.44%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YKI +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN ++ L++ IL +K   +VP++LVGNK DL +E    KE+  K +A ++NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKEL-GKNLATQFNCAFMETSAKAKVNVNDIFYDLV 161          
BLAST of Ras-like protein 3 vs. Ensembl Fly
Match: Rap1 (gene:FBgn0004636 transcript:FBtr0332671)

HSP 1 Score: 135.961 bits (341), Expect = 2.053e-38
Identity = 72/162 (44.44%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YKI +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN ++ L++ IL +K   +VP++LVGNK DL +E    KE+  K +A ++NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKEL-GKNLATQFNCAFMETSAKAKVNVNDIFYDLV 161          
BLAST of Ras-like protein 3 vs. Ensembl Fly
Match: Rala (gene:FBgn0015286 transcript:FBtr0070582)

HSP 1 Score: 113.62 bits (283), Expect = 8.820e-30
Identity = 63/171 (36.84%), Postives = 97/171 (56.73%), Query Frame = 1
Query:   58 KPLINPSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            KP   P+     +K+ ++G+ GVGKS L +QF+ +EF    E T  D Y+K + +     +I+I+DTAG E  +  +D Y    +G + V+SITD+ SF   +  ++ IL +K D ++P +LVGNK DL  + R+V     +  A +W   + E SAK   NV + F DLI
Sbjct:    4 KPTAGPAL----HKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDL-NDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFYDLI 169          
BLAST of Ras-like protein 3 vs. Ensembl Fly
Match: Rala (gene:FBgn0015286 transcript:FBtr0070583)

HSP 1 Score: 113.235 bits (282), Expect = 1.409e-29
Identity = 62/171 (36.26%), Postives = 97/171 (56.73%), Query Frame = 1
Query:   58 KPLINPSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            KP   P+     +K+ ++G+ GVGKS L +QF+ +EF    E T  D Y+K + +     +I+I+DTAG E  +  +D Y    +G + V+SITD+ SF   +  ++ IL +K D ++P +LVGNK DL  + R+V     +  A +W   + E SAK   NV + F DL+
Sbjct:    4 KPTAGPAL----HKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDL-NDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFFDLM 169          
BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Match: rap1aa (RAP1A, member of RAS oncogene family a [Source:ZFIN;Acc:ZDB-GENE-040625-167])

HSP 1 Score: 135.961 bits (341), Expect = 3.055e-38
Identity = 72/163 (44.17%), Postives = 104/163 (63.80%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL +E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDL-EEERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Match: rap1aa (RAP1A, member of RAS oncogene family a [Source:ZFIN;Acc:ZDB-GENE-040625-167])

HSP 1 Score: 135.961 bits (341), Expect = 3.055e-38
Identity = 72/163 (44.17%), Postives = 104/163 (63.80%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL +E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDL-EEERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Match: rap1b (RAP1B, member of RAS oncogene family [Source:NCBI gene;Acc:321107])

HSP 1 Score: 134.806 bits (338), Expect = 6.438e-38
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +WN C F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNSCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Match: rap1b (RAP1B, member of RAS oncogene family [Source:NCBI gene;Acc:321107])

HSP 1 Score: 133.65 bits (335), Expect = 1.781e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +WN C F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKAMEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNSCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Match: rap1ab (RAP1A, member of RAS oncogene family b [Source:ZFIN;Acc:ZDB-GENE-040426-2738])

HSP 1 Score: 132.109 bits (331), Expect = 7.038e-37
Identity = 71/163 (43.56%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVLDIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Match: aldh1a3 (aldehyde dehydrogenase 1 family member A3 [Source:Xenbase;Acc:XB-GENE-992885])

HSP 1 Score: 134.806 bits (338), Expect = 8.859e-38
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVEGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Match: rap1b (RAP1B, member of RAS oncogene family [Source:NCBI gene;Acc:493557])

HSP 1 Score: 134.806 bits (338), Expect = 1.087e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Match: aldh1a3 (aldehyde dehydrogenase 1 family member A3 [Source:Xenbase;Acc:XB-GENE-992885])

HSP 1 Score: 130.568 bits (327), Expect = 3.577e-36
Identity = 70/160 (43.75%), Postives = 101/160 (63.12%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFL 561
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVEGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEDFV 159          
BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Match: aldh1a3 (aldehyde dehydrogenase 1 family member A3 [Source:Xenbase;Acc:XB-GENE-992885])

HSP 1 Score: 127.872 bits (320), Expect = 3.646e-35
Identity = 69/157 (43.95%), Postives = 99/157 (63.06%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKE 552
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVEGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNE 156          
BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Match: rap2c (RAP2C, member of RAS oncogene family [Source:NCBI gene;Acc:549652])

HSP 1 Score: 124.79 bits (312), Expect = 5.783e-34
Identity = 70/162 (43.21%), Postives = 101/162 (62.35%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K I V  +   +EI+DTAGTE  +  +D YI    G ++VYS+ ++ SF  IK ++D I+ +K+   VPLILVGNK+DL  E R+V     + +A +W C F E SAK    V E F +++
Sbjct:    1 MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIVRVKRYEKVPLILVGNKVDLESE-REVMSAEGRSLAQEWGCPFMETSAKSKTMVDELFAEIV 161          
BLAST of Ras-like protein 3 vs. Ensembl Mouse
Match: Rap1b (RAS related protein 1b [Source:MGI Symbol;Acc:MGI:894315])

HSP 1 Score: 134.42 bits (337), Expect = 1.156e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Mouse
Match: Gm266 (predicted gene 266 [Source:MGI Symbol;Acc:MGI:2685112])

HSP 1 Score: 134.035 bits (336), Expect = 2.133e-37
Identity = 65/160 (40.62%), Postives = 95/160 (59.38%), Query Frame = 1
Query:   91 DYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            DY++ +LG+  VGK+ LA QF C  F  RCE ++E+L+ K I V+ A   +EIVDT G E +   +D YI  SDG VV+YS+ DE SF  ++ L++ +  ++    VPL+LVG K DL  E RQV     + +A +W C F E++ K    V   F  ++
Sbjct:   11 DYRVVVLGSVAVGKTALATQFACGRFPERCEPSVEELFSKVIEVNRAPALLEIVDTVGAEHLVTLKDLYIRNSDGFVVLYSVCDEASFQAVRPLRERMGRLRGSRAVPLVLVGTKADLDAE-RQVLTAQGRALAREWRCPFLEITTKSKTMVDHVFTQVV 169          
BLAST of Ras-like protein 3 vs. Ensembl Mouse
Match: Rap1a (RAS-related protein 1a [Source:MGI Symbol;Acc:MGI:97852])

HSP 1 Score: 132.494 bits (332), Expect = 5.096e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Mouse
Match: Rap1a (RAS-related protein 1a [Source:MGI Symbol;Acc:MGI:97852])

HSP 1 Score: 129.798 bits (325), Expect = 2.887e-36
Identity = 71/160 (44.38%), Postives = 101/160 (63.12%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFL 561
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E FL
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFL 159          
BLAST of Ras-like protein 3 vs. Ensembl Mouse
Match: Rap2b (RAP2B, member of RAS oncogene family [Source:MGI Symbol;Acc:MGI:1921262])

HSP 1 Score: 124.79 bits (312), Expect = 4.918e-34
Identity = 70/162 (43.21%), Postives = 104/162 (64.20%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K I V  +   +EI+DTAGTE  +  +D YI    G ++VYS+ ++ SF  IK ++D I+ +K+   VP+ILVGNK+DL  E R+V     K +A +W+C F E SAK+  +V E F +++
Sbjct:    1 MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGE-REVSYGEGKALAEEWSCPFMETSAKNKASVDELFAEIV 161          
BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Match: sp|P08645|RAP1_DROME (Ras-related protein Rap1 OS=Drosophila melanogaster OX=7227 GN=Rap1 PE=2 SV=2)

HSP 1 Score: 135.961 bits (341), Expect = 2.058e-37
Identity = 72/162 (44.44%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YKI +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN ++ L++ IL +K   +VP++LVGNK DL +E    KE+  K +A ++NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKEL-GKNLATQFNCAFMETSAKAKVNVNDIFYDLV 161          
BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Match: sp|A6NIZ1|RP1BL_HUMAN (Ras-related protein Rap-1b-like protein OS=Homo sapiens OX=9606 PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.147e-37
Identity = 72/163 (44.17%), Postives = 104/163 (63.80%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+RGVGKS L +QFV   F  + + TIED Y++ + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSRGVGKSALTVQFVQGIFVEKYDPTIEDSYREQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Match: sp|P32254|RASS_DICDI (Ras-like protein rasS OS=Dictyostelium discoideum OX=44689 GN=rasS PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.313e-37
Identity = 71/162 (43.83%), Postives = 101/162 (62.35%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M ++K+ L+G  GVGKSCL IQF+ ++F    + T+ED Y+K   V      ++I DTAG E  S  +D+Y+   +G + VYSIT   SF  I RL +H+L +K   +VP +LVGNK DL  E R+V     +E+A K NC F E SAK+ +NV E+F +L+
Sbjct:    1 MFNFKLVLVGPGGVGKSCLTIQFIAQKFVDEYDPTLEDSYRKQTTVDGEECLLDIYDTAGQEDFSAVRDQYMRTGEGFLCVYSITYLQSFKEIHRLHNHLLKVKDLDSVPFVLVGNKCDL-NEYREVSTAEGEELAKKLNCKFLETSAKERINVSESFYELV 161          
BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Match: sp|Q6TEN1|RAP1B_DANRE (Ras-related protein Rap-1b OS=Danio rerio OX=7955 GN=rap1b PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 4.860e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +WN C F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNSCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Match: sp|Q640R7|RAP1B_XENTR (Ras-related protein Rap-1b OS=Xenopus tropicalis OX=8364 GN=rap1b PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 5.759e-37
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. TrEMBL
Match: A0A267GI80 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023552g2 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.143e-38
Identity = 73/162 (45.06%), Postives = 104/162 (64.20%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +  +   +EI+DTAGTE  +  +D Y+    G ++VYSIT ++SFN +  L++ IL +K   NVP+I+VGNK DL  E R V +     +A +WNC F E SAK  LNV+E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGVFVEKYDPTIEDSYRKQVEIDSSQCMLEILDTAGTEQFTAMRDLYMKNGQGFLLVYSITSQSSFNDLAELREQILRVKDSDNVPIIVVGNKCDLEDE-RTVGKERGHSLARQWNCAFLETSAKAKLNVQEVFFDLV 161          
BLAST of Ras-like protein 3 vs. TrEMBL
Match: A0A267HBH2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013357g1 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.609e-38
Identity = 75/162 (46.30%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K I V +    +EI+DTAGTE  +  +D Y+    G ++VYSIT + SFN +  L+D IL +K   +VP+IL+GNK DL  E R V     + +A +WNC F E SAK  LNV+++FLDL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQIEVDNNQCMLEILDTAGTEQFTAMRDLYMKNGQGFLLVYSITSQASFNDLHELRDQILRVKDSDDVPIILIGNKCDLDDE-RTVSREQGQSLAHQWNCSFLETSAKAKLNVQDSFLDLV 161          
BLAST of Ras-like protein 3 vs. TrEMBL
Match: A0A1I8IV64 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023552g1 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 3.177e-38
Identity = 73/162 (45.06%), Postives = 104/162 (64.20%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +  +   +EI+DTAGTE  +  +D Y+    G ++VYSIT ++SFN +  L++ IL +K   NVP+I+VGNK DL  E R V +     +A +WNC F E SAK  LNV+E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDSSQCMLEILDTAGTEQFTAMRDLYMKNGQGFLLVYSITSQSSFNDLAELREQILRVKDSDNVPIIVVGNKCDLEDE-RTVGKERGHSLARQWNCAFLETSAKAKLNVQEVFFDLV 161          
BLAST of Ras-like protein 3 vs. TrEMBL
Match: A0A1I8IQF1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 3.316e-38
Identity = 73/162 (45.06%), Postives = 104/162 (64.20%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +  +   +EI+DTAGTE  +  +D Y+    G ++VYSIT ++SFN +  L++ IL +K   NVP+I+VGNK DL  E R V +     +A +WNC F E SAK  LNV+E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDSSQCMLEILDTAGTEQFTAMRDLYMKNGQGFLLVYSITSQSSFNDLAELREQILRVKDSDNVPIIVVGNKCDLEDE-RTVGKERGHSLARQWNCAFLETSAKAKLNVQEVFFDLV 161          
BLAST of Ras-like protein 3 vs. TrEMBL
Match: A0A1I8IKG4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006909g1 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 3.911e-38
Identity = 75/162 (46.30%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K I V +    +EI+DTAGTE  +  +D Y+    G ++VYSIT + SFN +  L+D IL +K   +VP+IL+GNK DL  E R V     + +A +WNC F E SAK  LNV+++FLDL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQIEVDNNQCMLEILDTAGTEQFTAMRDLYMKNGQGFLLVYSITSQASFNDLHELRDQILRVKDSDDVPIILIGNKCDLDDE-RTVSREQGQSLAHQWNCSFLETSAKAKLNVEDSFLDLV 161          
BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Match: rap1aa (RAP1A, member of RAS oncogene family [Source:NCBI gene;Acc:103047011])

HSP 1 Score: 134.806 bits (338), Expect = 5.382e-38
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Match: rap1b (RAP1B, member of RAS oncogene family [Source:NCBI gene;Acc:103035254])

HSP 1 Score: 134.806 bits (338), Expect = 6.329e-38
Identity = 72/163 (44.17%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +WN C F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNSCAFLESSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Match: rap1ab (ras-related protein Rap-1A [Source:NCBI gene;Acc:103022868])

HSP 1 Score: 132.109 bits (331), Expect = 5.840e-37
Identity = 71/163 (43.56%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +WN C F E SAK  +NV + F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNHCAFLESSAKSKINVLDIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Match: rap1aa (RAP1A, member of RAS oncogene family [Source:NCBI gene;Acc:103047011])

HSP 1 Score: 128.257 bits (321), Expect = 2.843e-35
Identity = 69/157 (43.95%), Postives = 99/157 (63.06%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKE 552
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVNE 156          
BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Match: rit1 (Ras like without CAAX 1 [Source:NCBI gene;Acc:103032482])

HSP 1 Score: 124.02 bits (310), Expect = 1.841e-33
Identity = 61/160 (38.12%), Postives = 92/160 (57.50%), Query Frame = 1
Query:   91 DYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +YK+ +LG  GVGKS + +QF+   F    + TIED YK  IR+ D    ++I+DTAG    +  +D+Y+   +G ++ YSITD  SF   +  K+ I  +++  + P++LVGNK DLA   RQV     KE+A ++ C F E SA     + E F  L+
Sbjct:   13 EYKLVMLGEGGVGKSAIIMQFISHRFPEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKELIYRVRRTVDTPVVLVGNKSDLA-HLRQVSMEEGKELAREFQCPFFETSAAFRYYIDEVFAALV 171          
BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Match: RIT2 (Ras like without CAAX 2 [Source:HGNC Symbol;Acc:HGNC:10017])

HSP 1 Score: 124.79 bits (312), Expect = 1.868e-34
Identity = 60/160 (37.50%), Postives = 91/160 (56.88%), Query Frame = 1
Query:   91 DYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +YKI +LG  GVGKS + +QF+   F    + TIED YK  +R+ D    ++I+DTAG    +  +D+Y+   +G ++ YSITD  SF    + K  I  ++    VP++LVGNK DL  E RQV +   + +A +++C F E SA     V E F  ++
Sbjct:   20 EYKIVMLGPGGVGKSAMTMQFISHRFPEDHDPTIEDAYKTQVRIDDETAHLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFQEAAQFKQLIYQVRHTYEVPVVLVGNKTDLG-EIRQVSQEEGRSLAREFSCQFFETSAALRQYVDEVFYAVV 178          
BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Match: ralba (v-ral simian leukemia viral oncogene homolog Ba (ras related) [Source:ZFIN;Acc:ZDB-GENE-040801-267])

HSP 1 Score: 115.931 bits (289), Expect = 3.392e-31
Identity = 61/159 (38.36%), Postives = 93/159 (58.49%), Query Frame = 1
Query:   94 YKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +K+ ++G+ GVGKS L +QF+ +EF    E T  D Y+K + +     +I+I+DTAG E  +  +D Y    +G + V+SI +  SF      ++ IL +K+D +VP +LVGNK DL +E RQV    A+  A +W   + E SAK   NV + F DL+
Sbjct:   14 HKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSICEHESFAATLEFREQILRVKEDDSVPFLLVGNKSDL-EERRQVSSEEARARAEQWRVSYVETSAKTRANVDKVFFDLM 171          
BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001560.1 (pep scaffold:Pmarinus_7.0:GL476528:157506:173211:1 gene:ENSPMAG00000001407.1 transcript:ENSPMAT00000001560.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 108.612 bits (270), Expect = 1.688e-28
Identity = 61/166 (36.75%), Postives = 95/166 (57.23%), Query Frame = 1
Query:   79 NKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLK--DHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            N +  +K+ ++G+ GVGKS L +QF+ +EF    E T  D Y+K + +     +I+I+DTAG E  +  +D Y    +G +  +SI +  SF     L+  + IL +K+D ++P +LVGNK DL +E RQV    A+  A +W   + E SAK   NV + F DL+
Sbjct:   10 NSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCAFSICEHESFAGTTDLRRSEQILRVKEDDSIPFLLVGNKSDL-EERRQVSIEEARARADQWGVGYVETSAKTRANVDKVFFDLM 174          
BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000518.1 (pep scaffold:Pmarinus_7.0:GL479781:11597:21619:-1 gene:ENSPMAG00000000465.1 transcript:ENSPMAT00000000518.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.219 bits (256), Expect = 1.840e-26
Identity = 63/167 (37.72%), Postives = 100/167 (59.88%), Query Frame = 1
Query:   73 PSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKD-DLNVKEAFLDLI 570
            P+  +  YK+ ++G  GVGKS L IQF  + F    + TIED Y K+  +      ++++DTAG E  S  +++Y+   DG ++VYS+TD+ SF  ++R    IL +K   + P+ILV NK+DL    R++     +E+A K++  + E SAKD  LNV +AF +L+
Sbjct:    7 PNENLPTYKVVVVGDGGVGKSALTIQFFQKIFVVDYDPTIEDSYIKHTEIDGQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVERFHQLILRVKDRDSFPMILVANKVDLM-HLRKISSEQGQEMAAKYSIPYIETSAKDPPLNVDKAFQELV 172          
BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Match: rheb (Ras homolog, mTORC1 binding [Source:ZFIN;Acc:ZDB-GENE-040426-1690])

HSP 1 Score: 101.679 bits (252), Expect = 3.815e-26
Identity = 61/158 (38.61%), Postives = 89/158 (56.33%), Query Frame = 1
Query:   97 KICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            KI +LG R VGKS L IQFV  +F    + TIE+ + KN+ V+     +++VDTAG +  S     Y     G V+VYS+T   SF +++ + + +L++    +VPL+LVGNK DL  E R +     K +A  WN  F E +AK+   V E F  +I
Sbjct:    8 KIAVLGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKNLTVNGQEFNLKLVDTAGQDEYSMFPQTYSMDIHGYVLVYSVTSIKSFEVVRVIHEKLLDMVGKVHVPLVLVGNKKDLHME-RVISCEEGKTLATSWNAAFMESTAKEHQTVVEVFKWMI 164          
BLAST of Ras-like protein 3 vs. Ensembl Yeast
Match: RSR1 (GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases [Source:SGD;Acc:S000003384])

HSP 1 Score: 122.094 bits (305), Expect = 4.728e-33
Identity = 66/163 (40.49%), Postives = 97/163 (59.51%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCL-FHELSAKDDLNVKEAFLDLI 570
            M DYK+ +LGA GVGKSCL +QFV   +    + TIED Y+K I + + +  +EI+DTAG    +  ++ YI    G ++VYS+TD  S   +  L++ +L IK    VP++L+GNK DL  E R +      E++ KW  + F+E SA    NV E F+DL+
Sbjct:    1 MRDYKLVVLGAGGVGKSCLTVQFVQGVYLDTYDPTIEDSYRKTIEIDNKVFDLEILDTAGIAQFTAMRELYIKSGMGFLLVYSVTDRQSLEELMELREQVLRIKDSDRVPMVLIGNKADLINE-RVISVEEGIEVSSKWGRVPFYETSALLRSNVDEVFVDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Yeast
Match: RAS1 (GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication [Source:SGD;Acc:S000005627])

HSP 1 Score: 108.612 bits (270), Expect = 1.173e-27
Identity = 65/160 (40.62%), Postives = 96/160 (60.00%), Query Frame = 1
Query:   91 DYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +YKI ++G  GVGKS L IQF+   F    + TIED Y+K + + D +  ++I+DTAG E  S  +++Y+   +G ++VYS+T  NSF+ +      I  +K    +P+++VGNKLDL  E RQV   +   +A + N  F E SAK  +NV EAF  LI
Sbjct:   10 EYKIVVVGGGGVGKSALTIQFIQSYFVDEYDPTIEDSYRKQVVIDDKVSILDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRNSFDELLSYYQQIQRVKDSDYIPVVVVGNKLDLENE-RQVSYEDGLRLAKQLNAPFLETSAKQAINVDEAFYSLI 168          
BLAST of Ras-like protein 3 vs. Ensembl Yeast
Match: RAS2 (GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication [Source:SGD;Acc:S000005042])

HSP 1 Score: 98.2117 bits (243), Expect = 7.404e-24
Identity = 66/169 (39.05%), Postives = 100/169 (59.17%), Query Frame = 1
Query:   61 PLINPSNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDL 567
            PL N SN + +YK+ ++G  GVGKS L IQ     F    + TIED Y+K + + D +  ++I+DTAG E  S  +++Y+   +G ++VYSIT ++S + +      IL +K    VP+++VGNK DL  E +QV   +   +A + N  F E SAK  +NV+EAF  L
Sbjct:    2 PL-NKSN-IREYKLVVVGGGGVGKSALTIQLTQSHFVDEYDPTIEDSYRKQVVIDDEVSILDILDTAGQEEYSAMREQYMRNGEGFLLVYSITSKSSLDELMTYYQQILRVKDTDYVPIVVVGNKSDLENE-KQVSYQDGLNMAKQMNAPFLETSAKQAINVEEAFYTL 167          
BLAST of Ras-like protein 3 vs. Ensembl Yeast
Match: YPT1 (Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles [Source:SGD;Acc:S000001856])

HSP 1 Score: 88.1965 bits (217), Expect = 4.275e-21
Identity = 63/159 (39.62%), Postives = 87/159 (54.72%), Query Frame = 1
Query:   94 YKICLLGARGVGKSCLAIQFVCEEFTSRCESTIE-DLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDL 567
            +K+ L+G  GVGKSCL ++F  + +T+   STI  D   K + +     K++I DTAG E        Y   S GI++VY +TD+ SFN +K     I +    S V  +LVGNK DL K+ R V+   AKE A      F E SA D  NV++AFL +
Sbjct:    9 FKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESFNGVKMWLQEI-DRYATSTVLKLLVGNKCDL-KDKRVVEYDVAKEFADANKMPFLETSALDSTNVEDAFLTM 165          
BLAST of Ras-like protein 3 vs. Ensembl Yeast
Match: RHB1 (Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins [Source:SGD;Acc:S000000622])

HSP 1 Score: 83.9593 bits (206), Expect = 1.484e-19
Identity = 59/169 (34.91%), Postives = 87/169 (51.48%), Query Frame = 1
Query:   97 KICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-----------NCLFHELSAKDDLNVKEAFLDLI 570
            KI L+GAR VGK+ L ++FV   F      TIE+ + + I        +EI+DTAG + +S    K ++   GI++ YSI +  SF+LI  L D +++     N+P+ILVG K DL +  + VK    K    K               F E SA+ D NV+E F+ L+
Sbjct:   18 KIALIGARNVGKTTLTVRFVESRFVESYYPTIENEFTRIIPYKSHDCTLEILDTAGQDEVSLLNIKSLTGVRGIILCYSIINRASFDLIPILWDKLVDQLGKDNLPVILVGTKADLGRSTKGVKRCVTKAEGEKLASTIGSQDKRNQAAFIECSAELDYNVEETFMLLL 186          
BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Match: EDO39047 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB93])

HSP 1 Score: 130.954 bits (328), Expect = 6.570e-37
Identity = 71/163 (43.56%), Postives = 104/163 (63.80%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G V+VYSIT +++FN ++ L++ IL +K   +VP++LVGNK DL  E R V +   + +A ++ NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEDE-RVVGKDQGQNLAKQFANCTFLETSAKAKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Match: EDO30836 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZN4])

HSP 1 Score: 116.316 bits (290), Expect = 2.205e-31
Identity = 67/162 (41.36%), Postives = 101/162 (62.35%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV  +F  + + TIED Y+K I V +    +EI+DTAGTE  +  +D YI    G ++VYS+ +  +F  +K ++  IL +K   NVP++LVGNK D+  E R+V    AK++A  W C  +E SAK   NV + F +++
Sbjct:    1 MKEYKVVVLGSGGVGKSALTVQFVTGQFVEKYDPTIEDFYRKEIEVDNNPSILEILDTAGTEQFASMRDLYIKNGQGFLLVYSLINRQTFADLKPMRAQILRVKGSENVPIVLVGNKSDIYDE-REVSVGEAKDLAEAWGCPLYETSAKTRSNVDKVFAEVV 161          
BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Match: EDO45827 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRV2])

HSP 1 Score: 112.464 bits (280), Expect = 8.924e-30
Identity = 60/159 (37.74%), Postives = 89/159 (55.97%), Query Frame = 1
Query:   94 YKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +K+ ++G+ GVGKS L +QF+ EEF    E T  D Y+K   +     +I+I+DTAG E  +  +D Y    +G + V+SIT+  SF      ++ IL +K D  +P +LVGNK DL  + RQV     +  A +W   + E SAK   NV + F DL+
Sbjct:   12 HKVIMVGSGGVGKSALTLQFMYEEFVEDYEPTKADSYRKKAILDGEECQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEPESFAATTEFREQILRVKGDDKIPFLLVGNKSDLT-DKRQVTSEEGQSKADEWGVPYVETSAKTRSNVDKVFFDLM 169          
BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Match: EDO36396 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIQ7])

HSP 1 Score: 111.694 bits (278), Expect = 1.767e-29
Identity = 55/154 (35.71%), Postives = 94/154 (61.04%), Query Frame = 1
Query:  109 LGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            LG  GVGKS L +Q +  +F    + TIED Y+  + + +    ++I+DTAG +  +  +++Y+   +G +++YSITD +SFNL  + KD I  +++  ++P++LVGNK DL +  R+V     +E+A +++C F E SA     V +AF  L+
Sbjct:    6 LGGGGVGKSALIVQLISHQFLDYHDPTIEDAYQHQVVLDNEPALLDILDTAGQQEFTAMREQYMRNGEGFIIIYSITDRSSFNLAAQYKDQIERVRRTDDIPIVLVGNKDDL-EGKREVTTQEGQELAHRFDCPFFETSACQRHFVDDAFHGLV 158          
BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Match: EDO39171 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAS1])

HSP 1 Score: 106.686 bits (265), Expect = 1.278e-27
Identity = 61/159 (38.36%), Postives = 95/159 (59.75%), Query Frame = 1
Query:   94 YKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            YK+ ++G  GVGKS L IQF+   F S  + TIED Y+K   + + +  ++I+DTAG E  S  +++Y+   +G ++V+S+TD +SF  I +  + IL +K  +  P+I+ GNK DL  E R V     +E+A +    + E SAK   NV +AF DL+
Sbjct:   12 YKLVVVGGGGVGKSALTIQFIQSHFVSDYDPTIEDSYRKQCVIDERVAHLDILDTAGQEEFSAMREQYMRSGEGFLLVFSVTDRSSFEEINKFYNQILRVKDRTEFPMIICGNKSDLEYE-RTVSTAEGQELARQLRIQYLETSAKQRTNVDQAFHDLV 169          
BLAST of Ras-like protein 3 vs. Ensembl Medaka
Match: rap1aa (ras-related protein O-Krev [Source:NCBI gene;Acc:101171247])

HSP 1 Score: 134.806 bits (338), Expect = 5.088e-38
Identity = 71/163 (43.56%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLETSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Medaka
Match: ENSORLT00000047032.1 (ras-related protein Rap-1b [Source:NCBI gene;Acc:101172864])

HSP 1 Score: 132.494 bits (332), Expect = 4.468e-37
Identity = 73/167 (43.71%), Postives = 105/167 (62.87%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKE----IALKWN-CLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL     +V+ V AKE    +A +WN C F E SAK  +NV E F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDL-----EVERVVAKESGIGLARQWNSCAFLETSAKSKINVNEIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Medaka
Match: rap1ab (ras-related protein Rap-1A [Source:NCBI gene;Acc:101167529])

HSP 1 Score: 132.109 bits (331), Expect = 5.928e-37
Identity = 71/163 (43.56%), Postives = 103/163 (63.19%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + V      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV + F DL+
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLESSAKSKINVLDIFYDLV 162          
BLAST of Ras-like protein 3 vs. Ensembl Medaka
Match: rap1aa (ras-related protein O-Krev [Source:NCBI gene;Acc:101171247])

HSP 1 Score: 128.642 bits (322), Expect = 1.918e-35
Identity = 68/157 (43.31%), Postives = 99/157 (63.06%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKW-NCLFHELSAKDDLNVKE 552
            M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL  E R V +   + +A +W NC F E SAK  +NV E
Sbjct:    1 MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDE-RVVGKEQGQNLARQWNNCAFLETSAKSKINVNE 156          
BLAST of Ras-like protein 3 vs. Ensembl Medaka
Match: ENSORLT00000030284.1 (ras-related protein Rap-1b [Source:NCBI gene;Acc:101172864])

HSP 1 Score: 127.872 bits (320), Expect = 1.459e-34
Identity = 71/164 (43.29%), Postives = 103/164 (62.80%), Query Frame = 1
Query:   76 SNKMVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKE----IALKWN-CLFHELSAKDDLNVKE 552
            S +M +YK+ +LG+ GVGKS L +QFV   F  + + TIED Y+K + +      +EI+DTAGTE  +  +D Y+    G  +VYSIT +++FN ++ L++ IL +K   +VP+ILVGNK DL     +V+ V AKE    +A +WN C F E SAK  +NV E
Sbjct:   17 SARMREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEIDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDL-----EVERVVAKESGIGLARQWNSCAFLETSAKSKINVNE 175          
BLAST of Ras-like protein 3 vs. Planmine SMEST
Match: SMESG000041477.1 (SMESG000041477.1)

HSP 1 Score: 333.183 bits (853), Expect = 1.305e-115
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLIXXXXXXXXXXXXXXXXCCQML 633
            MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLIKSIENKNSSKSTNSKKCCQML
Sbjct:    1 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLIKSIENKNSSKSTNSKKCCQML 183          
BLAST of Ras-like protein 3 vs. Planmine SMEST
Match: SMESG000017314.1 (SMESG000017314.1)

HSP 1 Score: 141.739 bits (356), Expect = 1.056e-40
Identity = 73/162 (45.06%), Postives = 107/162 (66.05%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG  GVGKS L +QFV   F  + + TIED Y+K + V  +   +EI+DTAGTE  +  +D Y+    G V+VYSIT ++SFN ++ L++ IL +K   NVP++LVGNK DL +++R V +   + +A K+NC F E SAK+ +NV E F DL+
Sbjct:    1 MKEYKLVVLGTGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGSQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSSFNELEDLREQILRVKDTDNVPMVLVGNKCDL-EDDRSVGKEQGQVLARKFNCSFMETSAKNKINVNEIFYDLV 161          
BLAST of Ras-like protein 3 vs. Planmine SMEST
Match: SMESG000017950.1 (SMESG000017950.1)

HSP 1 Score: 139.428 bits (350), Expect = 7.332e-40
Identity = 69/162 (42.59%), Postives = 105/162 (64.81%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M +YK+ +LG+ GVGKSCL +QFV   F  + + TIED Y+K + + D    +EI+DTAG E  +  +D Y+    G ++VYSIT++ SFN I  L++ I  IK  +++P+ILVGNK DL ++ R V  +  +  A  WNC F E SA+ + NV++ F++L+
Sbjct:    1 MREYKLVMLGSGGVGKSCLTVQFVQGVFLEKYDPTIEDSYRKQVEIDDNQCMLEILDTAGAEQFTAMRDMYMKSGQGFILVYSITNQTSFNDIDDLREQITRIKDSTDIPMILVGNKCDL-EDQRNVGRIQGQHKAKLWNCSFLETSARSNNNVQDIFVELV 161          
BLAST of Ras-like protein 3 vs. Planmine SMEST
Match: SMESG000017950.1 (SMESG000017950.1)

HSP 1 Score: 133.265 bits (334), Expect = 1.510e-37
Identity = 66/155 (42.58%), Postives = 100/155 (64.52%), Query Frame = 1
Query:  106 LLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            +LG+ GVGKSCL +QFV   F  + + TIED Y+K + + D    +EI+DTAG E  +  +D Y+    G ++VYSIT++ SFN I  L++ I  IK  +++P+ILVGNK DL ++ R V  +  +  A  WNC F E SA+ + NV++ F++L+
Sbjct:    1 MLGSGGVGKSCLTVQFVQGVFLEKYDPTIEDSYRKQVEIDDNQCMLEILDTAGAEQFTAMRDMYMKSGQGFILVYSITNQTSFNDIDDLREQITRIKDSTDIPMILVGNKCDL-EDQRNVGRIQGQHKAKLWNCSFLETSARSNNNVQDIFVELV 154          
BLAST of Ras-like protein 3 vs. Planmine SMEST
Match: SMESG000047013.1 (SMESG000047013.1)

HSP 1 Score: 126.716 bits (317), Expect = 5.431e-35
Identity = 63/162 (38.89%), Postives = 98/162 (60.49%), Query Frame = 1
Query:   85 MVDYKICLLGARGVGKSCLAIQFVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYISLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAKENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLI 570
            M  +KI +LG   VGKS + +Q++ E F      TIEDLY K    S    ++EI+DTAG +  +  +D YI   DG ++VY+I ++ +F  +  LK+HILN K+  ++PL+LVGNKLD A+  R V  ++ + +A  W C F E++A    +V+  F +L+
Sbjct:    1 MKQFKIVVLGHGDVGKSSITMQYISETFIDYYNPTIEDLYVKETEFSKIPVRLEILDTAGLDQFTTMRDLYIKNGDGFIIVYNIVNKKTFMNLNSLKEHILNTKRTKHIPLVLVGNKLDKARNRRAVDSIDGETLAAAWGCPFFEITAHCLEDVQNVFTNLL 162          
The following BLAST results are available for this feature:
BLAST of Ras-like protein 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RAP1B1.684e-3744.17RAP1B, member of RAS oncogene family [Source:HGNC ... [more]
RAP1B1.684e-3744.17RAP1B, member of RAS oncogene family [Source:HGNC ... [more]
RAP1B1.684e-3744.17RAP1B, member of RAS oncogene family [Source:HGNC ... [more]
RAP1B1.684e-3744.17RAP1B, member of RAS oncogene family [Source:HGNC ... [more]
RAP1A7.422e-3744.17RAP1A, member of RAS oncogene family [Source:HGNC ... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rap-14.049e-3744.44Ras-related protein Rap-1 [Source:UniProtKB/Swiss... [more]
rap-21.222e-3443.83RAP homolog (Vertebrate Rap GTPase family) [Sourc... [more]
ral-15.603e-3037.72RAL (Ras-related GTPase) homolog [Source:UniProtK... [more]
ral-11.267e-2937.72RAL (Ras-related GTPase) homolog [Source:UniProtK... [more]
rap-37.936e-2841.14RAP homolog (Vertebrate Rap GTPase family) [Sourc... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rap12.053e-3844.44gene:FBgn0004636 transcript:FBtr0303154[more]
Rap12.053e-3844.44gene:FBgn0004636 transcript:FBtr0072867[more]
Rap12.053e-3844.44gene:FBgn0004636 transcript:FBtr0332671[more]
Rala8.820e-3036.84gene:FBgn0015286 transcript:FBtr0070582[more]
Rala1.409e-2936.26gene:FBgn0015286 transcript:FBtr0070583[more]
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BLAST of Ras-like protein 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rap1aa3.055e-3844.17RAP1A, member of RAS oncogene family a [Source:ZFI... [more]
rap1aa3.055e-3844.17RAP1A, member of RAS oncogene family a [Source:ZFI... [more]
rap1b6.438e-3844.17RAP1B, member of RAS oncogene family [Source:NCBI ... [more]
rap1b1.781e-3744.17RAP1B, member of RAS oncogene family [Source:NCBI ... [more]
rap1ab7.038e-3743.56RAP1A, member of RAS oncogene family b [Source:ZFI... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aldh1a38.859e-3844.17aldehyde dehydrogenase 1 family member A3 [Source:... [more]
rap1b1.087e-3744.17RAP1B, member of RAS oncogene family [Source:NCBI ... [more]
aldh1a33.577e-3643.75aldehyde dehydrogenase 1 family member A3 [Source:... [more]
aldh1a33.646e-3543.95aldehyde dehydrogenase 1 family member A3 [Source:... [more]
rap2c5.783e-3443.21RAP2C, member of RAS oncogene family [Source:NCBI ... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rap1b1.156e-3744.17RAS related protein 1b [Source:MGI Symbol;Acc:MGI:... [more]
Gm2662.133e-3740.63predicted gene 266 [Source:MGI Symbol;Acc:MGI:2685... [more]
Rap1a5.096e-3744.17RAS-related protein 1a [Source:MGI Symbol;Acc:MGI:... [more]
Rap1a2.887e-3644.38RAS-related protein 1a [Source:MGI Symbol;Acc:MGI:... [more]
Rap2b4.918e-3443.21RAP2B, member of RAS oncogene family [Source:MGI S... [more]
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BLAST of Ras-like protein 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P08645|RAP1_DROME2.058e-3744.44Ras-related protein Rap1 OS=Drosophila melanogaste... [more]
sp|A6NIZ1|RP1BL_HUMAN2.147e-3744.17Ras-related protein Rap-1b-like protein OS=Homo sa... [more]
sp|P32254|RASS_DICDI2.313e-3743.83Ras-like protein rasS OS=Dictyostelium discoideum ... [more]
sp|Q6TEN1|RAP1B_DANRE4.860e-3744.17Ras-related protein Rap-1b OS=Danio rerio OX=7955 ... [more]
sp|Q640R7|RAP1B_XENTR5.759e-3744.17Ras-related protein Rap-1b OS=Xenopus tropicalis O... [more]
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BLAST of Ras-like protein 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GI802.143e-3845.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267HBH22.609e-3846.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IV643.177e-3845.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IQF13.316e-3845.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IKG43.911e-3846.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rap1aa5.382e-3844.17RAP1A, member of RAS oncogene family [Source:NCBI ... [more]
rap1b6.329e-3844.17RAP1B, member of RAS oncogene family [Source:NCBI ... [more]
rap1ab5.840e-3743.56ras-related protein Rap-1A [Source:NCBI gene;Acc:1... [more]
rap1aa2.843e-3543.95RAP1A, member of RAS oncogene family [Source:NCBI ... [more]
rit11.841e-3338.13Ras like without CAAX 1 [Source:NCBI gene;Acc:1030... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RIT21.868e-3437.50Ras like without CAAX 2 [Source:HGNC Symbol;Acc:HG... [more]
ralba3.392e-3138.36v-ral simian leukemia viral oncogene homolog Ba (r... [more]
ENSPMAT00000001560.11.688e-2836.75pep scaffold:Pmarinus_7.0:GL476528:157506:173211:1... [more]
ENSPMAT00000000518.11.840e-2637.72pep scaffold:Pmarinus_7.0:GL479781:11597:21619:-1 ... [more]
rheb3.815e-2638.61Ras homolog, mTORC1 binding [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
RSR14.728e-3340.49GTP-binding protein of the Ras superfamily; requir... [more]
RAS11.173e-2740.63GTPase involved in G-protein signaling in adenylat... [more]
RAS27.404e-2439.05GTP-binding protein; regulates nitrogen starvation... [more]
YPT14.275e-2139.62Rab family GTPase; involved in the ER-to-Golgi ste... [more]
RHB11.484e-1934.91Putative Rheb-related GTPase; involved in regulati... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO390476.570e-3743.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308362.205e-3141.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458278.924e-3037.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO363961.767e-2935.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391711.278e-2738.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ras-like protein 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rap1aa5.088e-3843.56ras-related protein O-Krev [Source:NCBI gene;Acc:1... [more]
ENSORLT00000047032.14.468e-3743.71ras-related protein Rap-1b [Source:NCBI gene;Acc:1... [more]
rap1ab5.928e-3743.56ras-related protein Rap-1A [Source:NCBI gene;Acc:1... [more]
rap1aa1.918e-3543.31ras-related protein O-Krev [Source:NCBI gene;Acc:1... [more]
ENSORLT00000030284.11.459e-3443.29ras-related protein Rap-1b [Source:NCBI gene;Acc:1... [more]
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BLAST of Ras-like protein 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041477.11.305e-115100.00SMESG000041477.1[more]
SMESG000017314.11.056e-4045.06SMESG000017314.1[more]
SMESG000017950.17.332e-4042.59SMESG000017950.1[more]
SMESG000017950.11.510e-3742.58SMESG000017950.1[more]
SMESG000047013.15.431e-3538.89SMESG000047013.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006167 ID=SMED30006167|Name=Ras-like protein 3|organism=Schmidtea mediterranea sexual|type=transcript|length=1047bp
AAATTATTTATAAATTATCTACACAAAAACTTTTTGTTGTGTTACTCAAA
AAATATTAAACCTCTAATTAATCCTAGCAATAAAATGGTTGACTATAAAA
TATGTTTGCTGGGCGCGAGAGGAGTTGGGAAAAGTTGCCTTGCCATTCAG
TTTGTCTGCGAAGAATTTACTAGTCGTTGTGAGTCTACTATAGAAGATCT
GTACAAGAAAAATATAAGAGTTTCAGATGCAATTAAAAAAATTGAAATCG
TCGATACAGCAGGGACGGAACTAATGTCACCAAACCAGGATAAGTACATT
TCTTTGAGTGATGGTATAGTTGTGGTCTACAGCATCACAGACGAAAATAG
TTTCAATTTGATAAAACGTTTAAAAGATCATATTCTGAATATAAAGCAAG
ATTCAAATGTACCTCTGATTCTTGTTGGCAATAAATTAGACCTGGCAAAA
GAGAATAGACAAGTAAAGGAAGTCAATGCTAAGGAAATCGCTTTGAAATG
GAATTGTTTATTTCATGAATTATCAGCTAAAGATGATTTAAATGTAAAAG
AGGCGTTCTTGGATTTAATTAAAAGCATAGAAAATAAAAATTCATCCAAG
TCAACCAACAGCAAGAAATGCTGCCAGATGTTGTGAAATCAAATCAAGAA
CTCTAAAATTTTCTGTCAAATAAATGATTTTGTTGGTGAAATATTGACAT
TGTTGCCGCTGCTGACTTTCAATTGAATCAGACCATTCGGACATTAGCAT
GCAATTTCTCTGAGATTTTCTGGACCCCTTGCAAACTATAATGTTTTTAA
AAAATTATTATAAACAATAAATGATAAAAATCGATAATTTTGTTTGATTT
CTGTTTTATCGTGGGAAAGAAAGATCAAACAGAGAAGGAGCAGAAATAGA
AAGACTTTATAGATTTTGTTTTGTTTTATGAGTGATCAGGGTCATGTTAA
GATCGACATATTTCATATTTTATGAATATACATTGCGGAAATTGCTTACA
CAGTTATTGATTAAATTTATGTTTTTATAAATGGTTCCGCTATCGCC
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protein sequence of SMED30006167-orf-1

>SMED30006167-orf-1 ID=SMED30006167-orf-1|Name=SMED30006167-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=212bp
KLFINYLHKNFLLCYSKNIKPLINPSNKMVDYKICLLGARGVGKSCLAIQ
FVCEEFTSRCESTIEDLYKKNIRVSDAIKKIEIVDTAGTELMSPNQDKYI
SLSDGIVVVYSITDENSFNLIKRLKDHILNIKQDSNVPLILVGNKLDLAK
ENRQVKEVNAKEIALKWNCLFHELSAKDDLNVKEAFLDLIKSIENKNSSK
STNSKKCCQML*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
GO:0000166nucleotide binding
GO:0019003GDP binding
GO:0019904protein domain specific binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR005225Small_GTP-bd_dom
IPR001806Small_GTPase
IPR027417P-loop_NTPase
IPR020849Small_GTPase_Ras-type
Vocabulary: biological process
TermDefinition
GO:0007264small GTPase mediated signal transduction
GO:0007165signal transduction
GO:0030168platelet activation
GO:0030336negative regulation of cell migration
GO:0031954positive regulation of protein autophosphorylation
GO:0032486Rap protein signal transduction
GO:0061097regulation of protein tyrosine kinase activity
GO:0070527platelet aggregation
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005768endosome
GO:0005829cytosol
GO:0005886plasma membrane
GO:0005923bicellular tight junction
GO:0044291cell-cell contact zone
GO:0045121membrane raft
GO:0055037recycling endosome
GO:0055038recycling endosome membrane
GO:0070062extracellular exosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 171..193
score: 40.51
coord: 55..71
score: 27.67
coord: 72..94
score: 31.09
coord: 135..148
score: 56.62
coord: 32..53
score: 43.92
NoneNo IPR availableSMARTSM00174rho_sub_3coord: 34..196
e-value: 3.0E-6
score: -37.8
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 32..196
e-value: 4.6E-23
score: 92.6
NoneNo IPR availableSMARTSM00173ras_sub_4coord: 29..196
e-value: 1.9E-55
score: 200.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 29..200
e-value: 2.2E-49
score: 169.3
NoneNo IPR availablePANTHERPTHR24070:SF226GTP-BINDING PROTEIN RHEB HOMOLOGcoord: 31..208
NoneNo IPR availableCDDcd00876Rascoord: 33..193
e-value: 4.00398E-63
score: 191.2
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 31..186
e-value: 1.7E-21
score: 74.6
IPR001806Small GTPasePFAMPF00071Rascoord: 33..194
e-value: 2.8E-43
score: 147.3
IPR020849Small GTPase superfamily, Ras-typePANTHERPTHR24070FAMILY NOT NAMEDcoord: 31..208
IPR020849Small GTPase superfamily, Ras-typePROSITEPS51421RAScoord: 27..211
score: 25.019
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 31..203