Twinkle mtDNA helicase

Overview
NameTwinkle mtDNA helicase
Smed IDSMED30006138
Length (bp)1213
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Twinkle mtDNA helicase (SMED30006138) t-SNE clustered cells

Violin plots show distribution of expression levels for Twinkle mtDNA helicase (SMED30006138) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Twinkle mtDNA helicase (SMED30006138) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Twinkle mtDNA helicase (SMED30006138) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006138

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30006138SMESG000049485.1 dd_Smed_v4_12427_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30006138SMESG000049485.1 dd_Smed_v4_45276_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Match: TWNK (twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGNC:1160])

HSP 1 Score: 239.195 bits (609), Expect = 8.633e-72
Identity = 113/279 (40.50%), Postives = 181/279 (64.87%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS------LSKTKDSS 936
            + + +++ ++  L+N  +  G  W R P+LN+ILKG R+GELTV +G TG+GKTTF+SEY+LDL  + GV TLWGSFE+S  +L+++MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G     S D+  AQD+ +G  R F  + N H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F         L K KD +
Sbjct:  369 SFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLC-SQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH-EQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDT 645
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Match: TWNK (twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGNC:1160])

HSP 1 Score: 197.593 bits (501), Expect = 5.580e-57
Identity = 87/214 (40.65%), Postives = 145/214 (67.76%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLA 768
            + + +++ ++  L+N  +  G  W R P+LN+ILKG R+GELTV +G TG+GKTTF+SEY+LDL  + GV TLWGSFE+S  +L+++MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G     S D+  AQD+ +G  R F  + N H++L+ HPRKE++ +EL + S++G  K++
Sbjct:  369 SFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLC-SQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH-EQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVS 580
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Match: TWNK (twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGNC:1160])

HSP 1 Score: 151.369 bits (381), Expect = 3.454e-40
Identity = 66/157 (42.04%), Postives = 109/157 (69.43%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQ 597
            + + +++ ++  L+N  +  G  W R P+LN+ILKG R+GELTV +G TG+GKTTF+SEY+LDL  + GV TLWGSFE+S  +L+++MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G 
Sbjct:  369 SFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLC-SQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH 524
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Match: TWNK (twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGNC:1160])

HSP 1 Score: 143.28 bits (360), Expect = 9.347e-40
Identity = 70/192 (36.46%), Postives = 116/192 (60.42%), Query Frame = 1 
Query:  388 MLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS------LSKTKDSS 936
            MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G     S D+  AQD+ +G  R F  + N H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F         L K KD +
Sbjct:    1 MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH-EQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDT 191
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Match: TWNK (twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGNC:1160])

HSP 1 Score: 101.293 bits (251), Expect = 2.748e-25
Identity = 44/127 (34.65%), Postives = 80/127 (62.99%), Query Frame = 1 
Query:  388 MLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLA 768
            MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G     S D+  AQD+ +G  R F  + N H++L+ HPRKE++ +EL + S++G  K++
Sbjct:    1 MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH-EQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVS 126
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Celegans
Match: twnk-1 (pep chromosome:WBcel235:X:5791703:5797328:1 gene:WBGene00018514.1 transcript:F46G11.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:twnk-1)

HSP 1 Score: 141.354 bits (355), Expect = 8.498e-37
Identity = 95/258 (36.82%), Postives = 148/258 (57.36%), Query Frame = 1 
Query:  133 KSIQQNIINSLTNDSELVG-KAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDE--EAITYYMEKFK----SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQ-----IGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ-----TIESQNRKYLQVVKNRHDG 855
            + I+ +++NS TN   +VG   W R   LNK L G R GE+TVL+G TG GKTTFL EYSLDL    GVRTL+ SFE+   K+   ML Q+AG  L + E   +I  ++++F+    ++ M   +    ++  EI + +R       I H+++DNLQF++ Q       S+  D+FH QD FVG +R    +  +H++++ HPRK +   E+      G  ++ QE+DN++ +Q        S+ RK+L ++KNR+ G
Sbjct:  289 EGIRSDLLNS-TN--RVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLF-TQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYG 542
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Celegans
Match: twnk-1 (pep chromosome:WBcel235:X:5792535:5797328:1 gene:WBGene00018514.1 transcript:F46G11.1d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:twnk-1)

HSP 1 Score: 141.354 bits (355), Expect = 8.628e-37
Identity = 95/258 (36.82%), Postives = 148/258 (57.36%), Query Frame = 1 
Query:  133 KSIQQNIINSLTNDSELVG-KAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDE--EAITYYMEKFK----SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQ-----IGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ-----TIESQNRKYLQVVKNRHDG 855
            + I+ +++NS TN   +VG   W R   LNK L G R GE+TVL+G TG GKTTFL EYSLDL    GVRTL+ SFE+   K+   ML Q+AG  L + E   +I  ++++F+    ++ M   +    ++  EI + +R       I H+++DNLQF++ Q       S+  D+FH QD FVG +R    +  +H++++ HPRK +   E+      G  ++ QE+DN++ +Q        S+ RK+L ++KNR+ G
Sbjct:  307 EGIRSDLLNS-TN--RVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLF-TQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYG 560
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Celegans
Match: twnk-1 (pep chromosome:WBcel235:X:5792535:5797328:1 gene:WBGene00018514.1 transcript:F46G11.1c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:twnk-1)

HSP 1 Score: 132.88 bits (333), Expect = 1.125e-33
Identity = 96/276 (34.78%), Postives = 149/276 (53.99%), Query Frame = 1 
Query:  133 KSIQQNIINSLTNDSELVG-KAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKS------------------LKKDE--EAITYYMEKFK----SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQ-----IGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ-----TIESQNRKYLQVVKNRHDG 855
            + I+ +++NS TN   +VG   W R   LNK L G R GE+TVL+G TG GKTTFL EYSLDL    GVRTL+ SFE+   K+   ML Q+AG S                  L + E   +I  ++++F+    ++ M   +    ++  EI + +R       I H+++DNLQF++ Q       S+  D+FH QD FVG +R    +  +H++++ HPRK +   E+      G  ++ QE+DN++ +Q        S+ RK+L ++KNR+ G
Sbjct:  307 EGIRSDLLNS-TN--RVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLF-TQGVRTLFCSFEMPEKKILHWMLVQYAGPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYG 578
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Celegans
Match: twnk-1 (pep chromosome:WBcel235:X:5791703:5797328:1 gene:WBGene00018514.1 transcript:F46G11.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:twnk-1)

HSP 1 Score: 128.642 bits (322), Expect = 2.671e-32
Identity = 95/285 (33.33%), Postives = 149/285 (52.28%), Query Frame = 1 
Query:  133 KSIQQNIINSLTNDSELVG-KAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAG-------------KSLKKDEE----------------AITYYMEKFK----SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQ-----IGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ-----TIESQNRKYLQVVKNRHDG 855
            + I+ +++NS TN   +VG   W R   LNK L G R GE+TVL+G TG GKTTFL EYSLDL    GVRTL+ SFE+   K+   ML Q+AG             +SL +                   +I  ++++F+    ++ M   +    ++  EI + +R       I H+++DNLQF++ Q       S+  D+FH QD FVG +R    +  +H++++ HPRK +   E+      G  ++ QE+DN++ +Q        S+ RK+L ++KNR+ G
Sbjct:  289 EGIRSDLLNS-TN--RVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLF-TQGVRTLFCSFEMPEKKILHWMLVQYAGYDDLVFFLFPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYG 569
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Fly
Match: mtDNA-helicase (gene:FBgn0032154 transcript:FBtr0079911)

HSP 1 Score: 240.736 bits (613), Expect = 3.169e-73
Identity = 115/277 (41.52%), Postives = 175/277 (63.18%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTP 948
            T  +++ +I++ L N  ++ G  W R P LNK+LKG R GELT+L+G TG+GKTTF+SEYSLDLA   GV TLWGSFE+  T+L+  +L Q+ G  L        ++  +F+ +P++F+   G++  K + + +  A   H + H+I+DNLQFM+G + +   DKF  QD  +   R+F  + NVH++L+ HPRKE  ++EL++ S++G  K  QE+DN+L++Q       + +KYLQ+ KNR+ G LG M L+F  D LS+ T +   K    KTP
Sbjct:  336 TFGAMRNDILSELQNIEKVNGVKWKRFPVLNKLLKGHRRGELTILTGPTGSGKTTFMSEYSLDLA-MQGVNTLWGSFEIRNTRLAATLLRQYVGYPLDDRLHEFNHWAAEFERLPLYFMTFHGQQPLKPVLEAIEHASYVHDVMHVIIDNLQFMMG-VSTFRGDKFFEQDSIIAAFRSFATKHNVHVTLVMHPRKERQEDELTTSSVFGTAKATQEADNVLIIQDKRLTSVRGKKYLQIAKNRYSGDLGIMPLEFDKDGLSYSTQIQNAKRKREKTP 610
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Zebrafish
Match: twnk (twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-030131-5569])

HSP 1 Score: 242.662 bits (618), Expect = 6.339e-73
Identity = 120/276 (43.48%), Postives = 179/276 (64.86%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            + K +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE +LDL    GV TLWGSFE++  +L+KIMLTQF+ + L+++ +    + +KF+ +P++F+   G++N K +   M+ A   + I H+I+DNLQFM+GQ  + S DKF  QDH +G  R F   +  H++LI HPRKE + +EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L+F   SL+F   + K K   RK 
Sbjct:  410 SFKQLREDVYGELANVEQVAGIQWSRFPELNRILKGHRKGELTVFTGPTGSGKTTFISECALDLCMQ-GVNTLWGSFEINNVRLAKIMLTQFSKQRLEENLDQYDMWADKFEDLPLYFMTFHGQQNIKTVLDTMQHAVYVYDISHVIIDNLQFMMGQ-ENLSVDKFAVQDHIIGIFRKFATNSCCHVTLIIHPRKEEDDKELQTASIFGTAKASQEADNVLILQEKKLVTCPGRRSLQVAKNRFDGDVGVFPLEFNKASLTFSAPV-KGKQKLRKV 682
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Zebrafish
Match: twnk (twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-030131-5569])

HSP 1 Score: 242.662 bits (618), Expect = 6.339e-73
Identity = 120/276 (43.48%), Postives = 179/276 (64.86%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            + K +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE +LDL    GV TLWGSFE++  +L+KIMLTQF+ + L+++ +    + +KF+ +P++F+   G++N K +   M+ A   + I H+I+DNLQFM+GQ  + S DKF  QDH +G  R F   +  H++LI HPRKE + +EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L+F   SL+F   + K K   RK 
Sbjct:  410 SFKQLREDVYGELANVEQVAGIQWSRFPELNRILKGHRKGELTVFTGPTGSGKTTFISECALDLCMQ-GVNTLWGSFEINNVRLAKIMLTQFSKQRLEENLDQYDMWADKFEDLPLYFMTFHGQQNIKTVLDTMQHAVYVYDISHVIIDNLQFMMGQ-ENLSVDKFAVQDHIIGIFRKFATNSCCHVTLIIHPRKEEDDKELQTASIFGTAKASQEADNVLILQEKKLVTCPGRRSLQVAKNRFDGDVGVFPLEFNKASLTFSAPV-KGKQKLRKV 682
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Xenopus
Match: mafg (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G [Source:Xenbase;Acc:XB-GENE-989896])

HSP 1 Score: 246.514 bits (628), Expect = 1.326e-74
Identity = 115/270 (42.59%), Postives = 180/270 (66.67%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTK 927
            + + +++ +   L N  ++ G  W R PELNK+LKG R+GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE++  +L+KIMLTQFA + L++  +    + ++F+ +P++F+   G++N K +   M+ A   + I H+I+DNLQFM+GQ  +   D+F AQD+ VG  R F  + N H++++ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    +++LQV KNR DG +G  +L+F    LSF ++  K K
Sbjct:  385 SFRQLREEVFGQLENADQVAGVKWARFPELNKLLKGHRKGELTVFTGPTGSGKTTFISEYALDLCIQ-GVNTLWGSFEINNVRLAKIMLTQFALQRLEEQLDKYDEWADRFEDLPLYFMTFHGQQNIKSVIDTMQHAVYMYDITHVIIDNLQFMMGQ-ENLYTDRFAAQDYMVGAFRKFATDNNCHVTVVIHPRKEDDDKELQTSSIFGSAKASQEADNVLILQDRKLVTGPGKRHLQVAKNRFDGDVGVFSLEFNKPCLSFASTKGKQK 652
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Xenopus
Match: mafg (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G [Source:Xenbase;Acc:XB-GENE-989896])

HSP 1 Score: 202.986 bits (515), Expect = 1.525e-58
Identity = 101/270 (37.41%), Postives = 158/270 (58.52%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTK 927
            + + +++ +   L N  ++ G  W R PELNK+LKG R+GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE++  +L+KIMLTQFA + L++  +    + ++F+ +P++F+   G++N                                      KF AQD+ VG  R F  + N H++++ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    +++LQV KNR DG +G  +L+F    LSF ++  K K
Sbjct:  385 SFRQLREEVFGQLENADQVAGVKWARFPELNKLLKGHRKGELTVFTGPTGSGKTTFISEYALDLCIQ-GVNTLWGSFEINNVRLAKIMLTQFALQRLEEQLDKYDEWADRFEDLPLYFMTFHGQQNI-------------------------------------KFAAQDYMVGAFRKFATDNNCHVTVVIHPRKEDDDKELQTSSIFGSAKASQEADNVLILQDRKLVTGPGKRHLQVAKNRFDGDVGVFSLEFNKPCLSFASTKGKQK 616
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Mouse
Match: Twnk (twinkle mtDNA helicase [Source:MGI Symbol;Acc:MGI:2137410])

HSP 1 Score: 235.728 bits (600), Expect = 1.354e-70
Identity = 110/277 (39.71%), Postives = 180/277 (64.98%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS------LSKTKD 930
            + + +++ ++  L+N  +  G  W R P+LN++LKG R+GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE+S  +L+++MLTQFA   L++  +    + ++F+ +P++F+   G+++ + +   M+ A   + + H+++DNLQFM+G     S+D+  AQD+ VG  R F  + + H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F         L K KD
Sbjct:  370 SFRQLREEVLGELSNVEQAAGVRWSRFPDLNRLLKGHRKGELTVFTGPTGSGKTTFISEYALDLC-TQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGH-EQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKD 644
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Mouse
Match: Twnk (twinkle mtDNA helicase [Source:MGI Symbol;Acc:MGI:2137410])

HSP 1 Score: 140.198 bits (352), Expect = 7.245e-39
Identity = 64/175 (36.57%), Postives = 112/175 (64.00%), Query Frame = 1 
Query:  388 MLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSF 903
            MLTQFA   L++  +    + ++F+ +P++F+   G+++ + +   M+ A   + + H+++DNLQFM+G     S+D+  AQD+ VG  R F  + + H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F
Sbjct:    1 MLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGH-EQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTF 174
[Close]
BLAST of Twinkle mtDNA helicase vs. UniProt/SwissProt
Match: sp|Q5ZIW1|PEO1_CHICK (Twinkle protein, mitochondrial OS=Gallus gallus OX=9031 GN=TWNK PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 9.216e-72
Identity = 113/262 (43.13%), Postives = 170/262 (64.89%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSF 903
            + + +++ +   L N  ++ G  W R PELNK+LKG R GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE++  +L+KIMLTQFA + L+   E    + ++F+ +P++F+   G++N K +   M+ A   + I H+++DNLQFM+G     S D+  AQD  VG  R F  +   H++L+ HPRKE++++EL + S++G  K +QE+DN+L+LQ         ++YLQV KNR DG +G   L+F   SL+F
Sbjct:  375 SFRQLREEVFGELANSEQVAGVKWARFPELNKLLKGHRRGELTVFTGPTGSGKTTFISEYALDLC-TQGVCTLWGSFEINNIRLAKIMLTQFATRRLEDQLELYDEWADRFEDLPLYFMTFHGQQNIKTVLDTMQHAVYMYDITHVVVDNLQFMMGH-EQLSADRLAAQDFIVGAFRKFATDNTCHITLVIHPRKEDDEKELQTASIFGSAKASQEADNVLILQDRKLTTGPGKRYLQVSKNRFDGDVGVFPLEFSKASLTF 634
[Close]
BLAST of Twinkle mtDNA helicase vs. UniProt/SwissProt
Match: sp|Q96RR1|PEO1_HUMAN (Twinkle protein, mitochondrial OS=Homo sapiens OX=9606 GN=TWNK PE=1 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 4.146e-71
Identity = 113/279 (40.50%), Postives = 181/279 (64.87%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS------LSKTKDSS 936
            + + +++ ++  L+N  +  G  W R P+LN+ILKG R+GELTV +G TG+GKTTF+SEY+LDL  + GV TLWGSFE+S  +L+++MLTQFA   L+   +   ++ ++F+ +P++F+   G+++ + +   M+ A   + I H+I+DNLQFM+G     S D+  AQD+ +G  R F  + N H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F         L K KD +
Sbjct:  369 SFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLC-SQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGH-EQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDT 645
[Close]
BLAST of Twinkle mtDNA helicase vs. UniProt/SwissProt
Match: sp|Q8CIW5|PEO1_MOUSE (Twinkle protein, mitochondrial OS=Mus musculus OX=10090 GN=Twnk PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 9.472e-70
Identity = 110/277 (39.71%), Postives = 180/277 (64.98%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS------LSKTKD 930
            + + +++ ++  L+N  +  G  W R P+LN++LKG R+GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE+S  +L+++MLTQFA   L++  +    + ++F+ +P++F+   G+++ + +   M+ A   + + H+++DNLQFM+G     S+D+  AQD+ VG  R F  + + H++L+ HPRKE++ +EL + S++G  K +QE+DN+L+LQ    +    ++YLQV KNR DG +G   L+F  +SL+F         L K KD
Sbjct:  370 SFRQLREEVLGELSNVEQAAGVRWSRFPDLNRLLKGHRKGELTVFTGPTGSGKTTFISEYALDLC-TQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGH-EQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKD 644
[Close]
BLAST of Twinkle mtDNA helicase vs. UniProt/SwissProt
Match: sp|P49519|DNAB_TRICV (Probable replicative DNA helicase OS=Trieres chinensis OX=1514140 GN=dnaB PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 8.532e-6
Identity = 58/254 (22.83%), Postives = 117/254 (46.06%), Query Frame = 1 
Query:  211 ELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFA---------GKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRA-AYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRK--ENN--KEELSSDSLYGGCKLAQESDNILLL---QTIESQNRKYLQ------VVKNRHDGGLGSMTLKFKSDSLSF 903
            +L+ + +GF++ +L +++G+   GKT F    + ++ +   +  L+ S E+S  +L+  +L+  A         G   KKD   +   ++   S+P FFI      + + I  +++   + ++ I  +++D LQ M      +SN +          L+N   E NV +  ++   +  EN   K  + SD    G  + Q++D +L+L   Q   S N    Q      ++  + +G +G++ LKF ++   F
Sbjct:  198 DLDSLTQGFQKSDLIIIAGRPSMGKTAFCLNIATNIVKKYKLPILFFSLEMSKEQLAYRLLSAEALINPMRLRNGHLNKKDWLKLHRIIKNLSSLP-FFIDDTPNLSIQAIRSKVKKLLFEQNTIGLVVIDYLQLMQSSTLKSSN-RVQELSQITRSLKNIAREFNVPVIALSQLSRNVENRIIKRPILSDLRESGS-IEQDADLVLMLYRDQYYNSNNENDEQLDITELILAKQRNGPIGTIQLKFDANYTKF 448
[Close]
BLAST of Twinkle mtDNA helicase vs. TrEMBL
Match: T1JTF3 (SF4 helicase domain-containing protein OS=Tetranychus urticae OX=32264 PE=4 SV=2)

HSP 1 Score: 250.751 bits (639), Expect = 3.618e-77
Identity = 126/275 (45.82%), Postives = 178/275 (64.73%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSN------DKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTI---ESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKT 924
            T   +++ + N + N  +L G  W R P +NK LKGFR GELTVL+G TG GKTTFLSEYSLDL    GV TLWG+FE+  T+L K M+ Q A K L  + +   ++ +KF  +PM+F+   G E F+ +   M+ A   H++ H+I+DNLQFM+G    T N      D++  QDH +G  R+F  E   H++L+ HPRKE++  EL+ +S++GG K +QE+DNIL+ Q      ++ RKYLQ+ KNR+DG LGS+TLKF  ++  FGTS SK+
Sbjct:   25 TFVQLRKGVYNEIVNHKQLEGIRWKRFPGINKYLKGFRPGELTVLTGPTGCGKTTFLSEYSLDLCL-QGVPTLWGNFEIQNTRLCKAMMNQLAQKQLSANLQEFDHWADKFTELPMYFMNYHGAEAFENVLATMKEAVTNHQVKHVIIDNLQFMMG----TRNKKYFHLDRYEYQDHVIGSCRSFATEKECHITLVIHPRKEDSG-ELTLNSIFGGAKASQEADNILIFQVEWKGNTRQRKYLQISKNRYDGDLGSITLKFNKETQGFGTSSSKS 293
[Close]
BLAST of Twinkle mtDNA helicase vs. TrEMBL
Match: W6U6G4 (Twinkle protein OS=Echinococcus granulosus OX=6210 GN=EGR_08368 PE=4 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 1.695e-76
Identity = 128/258 (49.61%), Postives = 170/258 (65.89%), Query Frame = 1 
Query:  154 INSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLK-KDEEAITYYMEKFK-SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTS-NDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ--TIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS 912
            +N + N  E+ G  W R  ELNK+L+GFR  E+TVLSGKTG GKTTF  EYSLDLAE  GVRTLWGSFE+ + ++ + +L QFAG+ L  +    +  +   F+  IPMFFI T GR + KE+F  +  + +   ++H+ILDNLQFM+G  GS    DKF  QD FV +LR F  +T  HL+L+ HPRK ++ + L+  SLYGG K+ QE+DNILL+Q     +  +KYLQVVKNR+DG +G M L F    +SF  S
Sbjct:   56 LNEILNVVEIQGIQWQRFKELNKLLRGFRSHEMTVLSGKTGVGKTTFACEYSLDLAEQ-GVRTLWGSFEMPLPRICRTLLHQFAGEQLYLQTPMRVAAWASAFQHEIPMFFINTHGRPSEKEVFLALEESVKRDAVEHVILDNLQFMMGCTGSQGIEDKFQRQDRFVERLRAFATDTGAHLTLVVHPRKVDSDQLLTISSLYGGGKIGQEADNILLVQEEVDTAVPKKYLQVVKNRYDGTVGKMDLHFNRSRMSFKPS 312
[Close]
BLAST of Twinkle mtDNA helicase vs. TrEMBL
Match: A0A0S7K350 (PEO1 (Fragment) OS=Poeciliopsis prolifica OX=188132 GN=PEO1 PE=4 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 2.559e-75
Identity = 120/272 (44.12%), Postives = 180/272 (66.18%), Query Frame = 1 
Query:  139 IQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE++LDL    GV TLWGSFE++  +L+K+MLTQFA + L+++ E   ++ ++F+ +P++F+   G++N K +   M  A   + I+H+I+DNLQFM+GQ  + S DKF  QDH +G  R F   ++ H++LI HPRKE + +EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L F   SL+F T + K K   RK 
Sbjct:    7 LREDVYGELMNTDQVAGVKWARFPELNRILKGHRKGELTVFTGPTGSGKTTFISEFALDLC-MQGVNTLWGSFEINNVRLAKVMLTQFAMQRLEENLEQYDFWADRFEELPLYFMTFHGQQNIKAVLDTMHHAVYLYDINHVIIDNLQFMMGQ-ENLSVDKFAVQDHIIGMFRKFATNSSCHVTLIIHPRKEEDDKELQTASIFGSAKASQEADNVLILQEKKLVTCPGRRSLQVTKNRFDGDVGIFPLDFIKSSLTFSTPI-KGKHKLRKV 275
[Close]
BLAST of Twinkle mtDNA helicase vs. TrEMBL
Match: A0A5K3FEQ8 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 4.039e-75
Identity = 126/258 (48.84%), Postives = 170/258 (65.89%), Query Frame = 1 
Query:  154 INSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLK-KDEEAITYYMEKFK-SIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTS-NDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ--TIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTS 912
            +N + N  EL G  W R  ELNKILKGFR  E+TVLSGKTG GKTTF  EYSLDLAE  GV+TLWGSFE+++ +L + +L QFAG+ L  +    +  +   F+  +PMFF+   GR + KE+F  +  + ++H I+H+ILDNLQFM+G   +    +KF  QD FV QLR F   T  HL+++ HPRK ++   L+  SLYGG K++QE+DN+LLLQ     +  +KY+QVVKNR+DG  G + L F  D +SF  S
Sbjct:   15 LNEILNVVELQGVQWRRFKELNKILKGFRSHEMTVLSGKTGIGKTTFACEYSLDLAER-GVKTLWGSFEMTLPRLCRTLLHQFAGEPLHFQQPMRVAAWASAFREEVPMFFLNMHGRPSEKEVFSALEESTQKHAIEHVILDNLQFMMGSSDTRRMEEKFQRQDRFVEQLRGFTTRTGAHLTVVVHPRKTDDDHLLTIASLYGGGKISQEADNVLLLQEEVGTAVPKKYIQVVKNRYDGTQGKVDLHFNRDRMSFRPS 271
[Close]
BLAST of Twinkle mtDNA helicase vs. TrEMBL
Match: A0A1A7XZZ3 (Chromosome 10 open reading frame 2 (Fragment) OS=Iconisemion striatum OX=60296 GN=C10ORF2 PE=4 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 4.293e-75
Identity = 123/277 (44.40%), Postives = 182/277 (65.70%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQ---TIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTP 948
            + K +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE +LDL    GV TLWGSFE++  +L+KIMLTQFA + L+++ E   ++ +KF+ +P++F+   G++N K +   M+ A   + I+H+++DNLQFM+GQ  + S DKF  QDH +G  R F   ++ H++LI HPRKE +  EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L F   SL+F T + K K   RK P
Sbjct:  207 SFKQLREDVYGELVNTDQVAGVKWARFPELNRILKGHRKGELTVFTGPTGSGKTTFISELALDLCMQ-GVNTLWGSFEINNVRLAKIMLTQFAMQRLEENLEHYDFWADKFEELPLYFMTFHGQQNIKTVLDTMQHAVYLYDINHVVIDNLQFMMGQ-ENLSVDKFAVQDHIIGAFRKFATNSSCHVTLIIHPRKEEDDRELQTASIFGSAKASQEADNVLILQEKKVVTCPGRRSLQVTKNRFDGDVGIFPLDFIKTSLTFSTPI-KGKHKLRKVP 480
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Cavefish
Match: twnk (twinkle mtDNA helicase [Source:NCBI gene;Acc:103026372])

HSP 1 Score: 241.891 bits (616), Expect = 1.026e-72
Identity = 117/276 (42.39%), Postives = 179/276 (64.86%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            + K +++++   L N  ++ G  W R PEL +ILKG R GELTV +G TG+GKTTF+SEY+LDL    GV TLWGSFE+   +L++IMLTQFA + L+++ +    + +KF+ +P++F+   G++N K +   M+ A   + I H+++DNLQFM+GQ  + S DKF  QDH +G  R F   ++ H++LI HPRKE + +EL + S++G  K +QE+DN+L+LQ    +    R+ +QV KNR DG +G   L+F   +L+F + + K K   RK 
Sbjct:  411 SFKQLREDVYGELANMEQVAGMKWTRFPELTRILKGHRNGELTVFTGPTGSGKTTFISEYALDLCMQ-GVNTLWGSFEIKNVRLARIMLTQFAMQRLEENLDQYDSWADKFEELPLYFMTFHGQQNIKTVLDTMQHAVYLYDISHVVIDNLQFMMGQ-ENLSVDKFAVQDHIIGAFRKFATNSSCHVTLIIHPRKEEDDKELQTASIFGSAKASQEADNVLILQEKKLVTCPGRRSMQVAKNRFDGDVGVFALEFNKATLTFSSPV-KGKQKLRKV 683
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Sea Lamprey
Match: twnk (twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-030131-5569])

HSP 1 Score: 237.654 bits (605), Expect = 1.554e-72
Identity = 111/260 (42.69%), Postives = 174/260 (66.92%), Query Frame = 1 
Query:  133 KSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIE---SQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSF 903
            + +++++   L N  ++ G  W R P LNK+LKG R+GELT+ +G TG+GKTTF+SEY+LDLA + GV TLWGSFE+   +L+++MLTQ+A + L+ + ++   + ++F+ +P+FF+   G++N K +   M+ A   + I H+++DNLQFM+G + + S D+F  QD  VG  R F      H++L+ HPRKE+   EL + S++G  K +QE+DN+L+LQ      +  +KYLQ+ KNR DG LGS+ L+F   SL+F
Sbjct:  377 RQLREDVRGELANSEQVAGVKWQRFPALNKLLKGHRKGELTIFTGPTGSGKTTFISEYALDLA-SQGVSTLWGSFEIGNVRLARVMLTQYARQRLESNIDSYDEWADRFEDLPLFFMTFHGQQNIKTVLDTMQHAVYMYDISHVVIDNLQFMLG-LQNPSMDRFTMQDFVVGAFRKFATVNMCHVTLVIHPRKEDFDRELQTASIFGTAKASQEADNVLILQDRRLSATHGKKYLQLAKNRFDGDLGSIPLEFDKASLTF 634
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BLAST of Twinkle mtDNA helicase vs. Ensembl Nematostella
Match: EDO43259 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZD6])

HSP 1 Score: 238.81 bits (608), Expect = 1.629e-75
Identity = 114/259 (44.02%), Postives = 167/259 (64.48%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSF 903
            T   ++  +   L N  ++ G  W R P+LN ILKG R GELT+ +G TG GKTT LSE SLDL + SGV TLWGSFE+   +L K ML Q +G +L+K+ +  TY+ +KF+ +PM+F+   G +N   + + M  A   + IDHII+DNLQFM   I   S+D++   +  +G  R+F +  NVH++L+ HPRKEN+  EL + S++G  K +QE+DN+++LQ    ++ KYLQV KNR DG +G + L+F  +S+S 
Sbjct:  113 TFHQLRNEVYGELVNADQVAGVKWSRFPKLNSILKGHRRGELTIFTGPTGAGKTTLLSELSLDLCQ-SGVNTLWGSFEIRNVRLIKTMLNQLSGINLQKNVQQFTYWADKFEMLPMYFMCFYGSQNINSVVETMAHAAYVYDIDHIIIDNLQFMTSNI--RSDDRYSVHNQAIGAFRDFASTKNVHVTLVIHPRKENDDVELQTASIFGTAKASQEADNVMILQDKRGRSDKYLQVTKNRFDGTVGRIPLEFVKESISM 368
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BLAST of Twinkle mtDNA helicase vs. Ensembl Nematostella
Match: EDO26183 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCN3])

HSP 1 Score: 111.694 bits (278), Expect = 6.368e-30
Identity = 52/126 (41.27%), Postives = 80/126 (63.49%), Query Frame = 1 
Query:  334 VRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRK 711
            V TLWGSFE+   +L K ML Q +G +L+K+ +  TY+ +KF+ +PM+F+   G +N   + + M  A   + IDHII+DNLQFM   I   S+D++   +  +G  R+F +  NVH++L+ HPRK
Sbjct:    1 VNTLWGSFEIRNVRLIKTMLNQLSGINLQKNVQQFTYWADKFEMLPMYFMCFYGSQNINSVVETMAHAAYVYDIDHIIIDNLQFMTSNI--RSDDRYSVHNQAIGAFRDFASTKNVHVTLVIHPRK 124
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BLAST of Twinkle mtDNA helicase vs. Ensembl Medaka
Match: twnk (twinkle mtDNA helicase [Source:NCBI gene;Acc:101170228])

HSP 1 Score: 247.284 bits (630), Expect = 3.569e-75
Identity = 125/276 (45.29%), Postives = 179/276 (64.86%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            + K +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE +LDL    GV TLWGSFE++  +L+KIMLTQFA + L+ + E   ++ +KF+ +P++F+   G++N K +   M  A   + I+HII+DNLQFM+GQ  + S DKF  QDH +G  R F   ++ H++LI HPRKE +  EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L F   SL+F T  SK K   RK 
Sbjct:  353 SFKQLREDVYGELLNTDQVAGVKWTRFPELNRILKGHRKGELTVFTGPTGSGKTTFISELALDLCMQ-GVNTLWGSFEINNVRLAKIMLTQFAMERLEDNLEKYDFWADKFEQLPLYFMTFHGQQNIKTVLDTMEHAVYLYDINHIIIDNLQFMMGQ-ENLSIDKFAVQDHIIGVFRKFATNSSCHVTLIIHPRKEEDDRELQTASIFGSAKASQEADNVLILQEKKLVTCPGRRSLQVTKNRFDGDVGIFPLDFIKASLTFSTP-SKGKHKLRKV 625
[Close]
BLAST of Twinkle mtDNA helicase vs. Ensembl Medaka
Match: twnk (twinkle mtDNA helicase [Source:NCBI gene;Acc:101170228])

HSP 1 Score: 247.669 bits (631), Expect = 8.029e-75
Identity = 125/276 (45.29%), Postives = 179/276 (64.86%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQT---IESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKT 945
            + K +++++   L N  ++ G  W R PELN+ILKG R+GELTV +G TG+GKTTF+SE +LDL    GV TLWGSFE++  +L+KIMLTQFA + L+ + E   ++ +KF+ +P++F+   G++N K +   M  A   + I+HII+DNLQFM+GQ  + S DKF  QDH +G  R F   ++ H++LI HPRKE +  EL + S++G  K +QE+DN+L+LQ    +    R+ LQV KNR DG +G   L F   SL+F T  SK K   RK 
Sbjct:  400 SFKQLREDVYGELLNTDQVAGVKWTRFPELNRILKGHRKGELTVFTGPTGSGKTTFISELALDLCMQ-GVNTLWGSFEINNVRLAKIMLTQFAMERLEDNLEKYDFWADKFEQLPLYFMTFHGQQNIKTVLDTMEHAVYLYDINHIIIDNLQFMMGQ-ENLSIDKFAVQDHIIGVFRKFATNSSCHVTLIIHPRKEEDDRELQTASIFGSAKASQEADNVLILQEKKLVTCPGRRSLQVTKNRFDGDVGIFPLDFIKASLTFSTP-SKGKHKLRKV 672
[Close]
BLAST of Twinkle mtDNA helicase vs. Planmine SMEST
Match: SMESG000049485.1 (SMESG000049485.1)

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1 
Query:  127 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLIXXXXXXXXXXXXXXXXXXTNYNIQKAFG 1038
            TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLISQGNGNSKQDQNNSSNSDTNYNIQKAFG
Sbjct:    5 TMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLISQGNGNSKQDQNNSSNSDTNYNIQKAFG 308
[Close]
BLAST of Twinkle mtDNA helicase vs. Planmine SMEST
Match: SMESG000049485.1 (SMESG000049485.1)

HSP 1 Score: 578.941 bits (1491), Expect = 0.000e+0
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1 
Query:  130 MKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLIXXXXXXXXXXXXXXXXXXTNYNIQKAFG 1038
            MKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLISQGNGNSKQDQNNSSNSDTNYNIQKAFG
Sbjct:    1 MKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLSGKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKSLKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHIILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKENNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLGSMTLKFKSDSLSFGTSLSKTKDSSRKTPLISQGNGNSKQDQNNSSNSDTNYNIQKAFG 303
The following BLAST results are available for this feature:
BLAST of Twinkle mtDNA helicase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TWNK8.633e-7240.50twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGN... [more]
TWNK5.580e-5740.65twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGN... [more]
TWNK3.454e-4042.04twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGN... [more]
TWNK9.347e-4036.46twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGN... [more]
TWNK2.748e-2534.65twinkle mtDNA helicase [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
twnk-18.498e-3736.82pep chromosome:WBcel235:X:5791703:5797328:1 gene:W... [more]
twnk-18.628e-3736.82pep chromosome:WBcel235:X:5792535:5797328:1 gene:W... [more]
twnk-11.125e-3334.78pep chromosome:WBcel235:X:5792535:5797328:1 gene:W... [more]
twnk-12.671e-3233.33pep chromosome:WBcel235:X:5791703:5797328:1 gene:W... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mtDNA-helicase3.169e-7341.52gene:FBgn0032154 transcript:FBtr0079911[more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
twnk6.339e-7343.48twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
twnk6.339e-7343.48twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mafg1.326e-7442.59v-maf avian musculoaponeurotic fibrosarcoma oncoge... [more]
mafg1.525e-5837.41v-maf avian musculoaponeurotic fibrosarcoma oncoge... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Twnk1.354e-7039.71twinkle mtDNA helicase [Source:MGI Symbol;Acc:MGI:... [more]
Twnk7.245e-3936.57twinkle mtDNA helicase [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Twinkle mtDNA helicase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q5ZIW1|PEO1_CHICK9.216e-7243.13Twinkle protein, mitochondrial OS=Gallus gallus OX... [more]
sp|Q96RR1|PEO1_HUMAN4.146e-7140.50Twinkle protein, mitochondrial OS=Homo sapiens OX=... [more]
sp|Q8CIW5|PEO1_MOUSE9.472e-7039.71Twinkle protein, mitochondrial OS=Mus musculus OX=... [more]
sp|P49519|DNAB_TRICV8.532e-622.83Probable replicative DNA helicase OS=Trieres chine... [more]
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BLAST of Twinkle mtDNA helicase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1JTF33.618e-7745.82SF4 helicase domain-containing protein OS=Tetranyc... [more]
W6U6G41.695e-7649.61Twinkle protein OS=Echinococcus granulosus OX=6210... [more]
A0A0S7K3502.559e-7544.12PEO1 (Fragment) OS=Poeciliopsis prolifica OX=18813... [more]
A0A5K3FEQ84.039e-7548.84Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A1A7XZZ34.293e-7544.40Chromosome 10 open reading frame 2 (Fragment) OS=I... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
twnk1.026e-7242.39twinkle mtDNA helicase [Source:NCBI gene;Acc:10302... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
twnk1.554e-7242.69twinkle mtDNA helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Twinkle mtDNA helicase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO432591.629e-7544.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO261836.368e-3041.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Twinkle mtDNA helicase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
twnk3.569e-7545.29twinkle mtDNA helicase [Source:NCBI gene;Acc:10117... [more]
twnk8.029e-7545.29twinkle mtDNA helicase [Source:NCBI gene;Acc:10117... [more]
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BLAST of Twinkle mtDNA helicase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000049485.10.000e+0100.00SMESG000049485.1[more]
SMESG000049485.10.000e+0100.00SMESG000049485.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006138 ID=SMED30006138|Name=Twinkle mtDNA helicase|organism=Schmidtea mediterranea sexual|type=transcript|length=1213bp
CTTTAATAATTACTTATTAAGTTTTTATACAATTTAGCACAAAATTATTC
AATACGATTTTATACCTCTTTTAATTTTATATGCGGCTAAATTGTATATA
ATTACATTATAATAATGGTTATAATTACCATGAAGAGTATTCAACAGAAT
ATTATAAACTCTTTAACTAATGATTCAGAGTTAGTTGGTAAAGCATGGCA
TCGATTACCTGAATTAAATAAGATATTGAAAGGATTTCGAGAAGGAGAAT
TGACAGTTCTAAGTGGGAAAACAGGCACAGGCAAAACGACATTTTTGTCA
GAATATTCTTTAGATTTAGCAGAGAATTCTGGTGTTAGAACTCTTTGGGG
AAGTTTTGAAGTGTCAATTACTAAATTAAGTAAAATCATGTTGACTCAAT
TTGCCGGAAAATCACTGAAAAAAGATGAAGAAGCAATCACTTATTATATG
GAAAAGTTTAAAAGTATTCCAATGTTTTTTATTCAAACTGAAGGCCGTGA
AAATTTCAAAGAAATATTTCAGGAAATGAGAGCTGCGTACCGAGAACATA
AAATTGACCATATCATTTTGGACAATTTGCAATTTATGGTCGGACAAATT
GGGAGTACATCTAACGATAAATTCCACGCTCAAGACCATTTTGTGGGCCA
ACTGAGAAACTTTGTCAATGAAACCAATGTCCATTTGTCTTTGATTGCTC
ATCCGCGCAAGGAAAACAACAAGGAAGAATTATCGAGTGACAGTCTATAC
GGGGGCTGCAAATTAGCACAGGAATCAGATAACATATTACTCTTACAAAC
TATTGAAAGTCAAAATCGTAAATACCTGCAAGTTGTGAAAAACCGTCATG
ACGGCGGATTAGGATCCATGACATTAAAGTTCAAATCAGATTCGTTGAGT
TTCGGTACCAGCTTATCGAAAACAAAAGATAGCTCCAGGAAAACTCCACT
AATATCACAAGGCAATGGAAACTCGAAACAAGATCAAAACAATAGTTCAA
ATTCAGATACAAATTATAATATTCAAAAAGCATTTGGATAAATTTCCCAG
GAAATGATTTATTTTATAATTTTAATGAGATGTGACATCTATCGATAAAG
GCATATTTTTCTATCAAATTTATTTTCGTGATCTTCGGACAGTGAAATTG
TGCAAATTTCATTTCGTAACTGTTGAGAGCGGATTTATTCAGTTTTTTGA
TATTTCTCTAAGC
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protein sequence of SMED30006138-orf-1

>SMED30006138-orf-1 ID=SMED30006138-orf-1|Name=SMED30006138-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=309bp
MVIITMKSIQQNIINSLTNDSELVGKAWHRLPELNKILKGFREGELTVLS
GKTGTGKTTFLSEYSLDLAENSGVRTLWGSFEVSITKLSKIMLTQFAGKS
LKKDEEAITYYMEKFKSIPMFFIQTEGRENFKEIFQEMRAAYREHKIDHI
ILDNLQFMVGQIGSTSNDKFHAQDHFVGQLRNFVNETNVHLSLIAHPRKE
NNKEELSSDSLYGGCKLAQESDNILLLQTIESQNRKYLQVVKNRHDGGLG
SMTLKFKSDSLSFGTSLSKTKDSSRKTPLISQGNGNSKQDQNNSSNSDTN
YNIQKAFG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR007694DNA_helicase_DnaB-like_C
Vocabulary: molecular function
TermDefinition
GO:0003678DNA helicase activity
GO:0005524ATP binding
GO:0000166nucleotide binding
GO:0003697single-stranded DNA binding
GO:0004386helicase activity
GO:0016787hydrolase activity
GO:00431395'-3' DNA helicase activity
Vocabulary: biological process
TermDefinition
GO:0006260DNA replication
GO:0006264mitochondrial DNA replication
GO:0032508DNA duplex unwinding
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0042645mitochondrial nucleoid
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13481AAA_25coord: 29..201
e-value: 3.0E-24
score: 85.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 11..264
e-value: 6.1E-47
score: 162.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..308
NoneNo IPR availablePANTHERPTHR12873:SF0TWINKLE PROTEIN, MITOCHONDRIALcoord: 3..292
NoneNo IPR availablePANTHERPTHR12873T7-LIKE MITOCHONDRIAL DNA HELICASEcoord: 3..292
IPR007694DNA helicase, DnaB-like, C-terminalPROSITEPS51199SF4_HELICASEcoord: 20..270
score: 44.377
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 31..256