SMED30006127

Overview
NameSMED30006127
Smed IDSMED30006127
Length (bp)2753
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30006127 (SMED30006127) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30006127 (SMED30006127) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30006127 (SMED30006127) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30006127 (SMED30006127) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006127

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30006127SMESG000018155.1 SmedASXL_014614SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30006127SMESG000018155.1 SmedASXL_014614SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30006127 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007956:1046..5175 -11640
Homology
[Close]
BLAST of SMED30006127 vs. TrEMBL
Match: A0A0V0J9X9 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR91075 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.070e-19
Identity = 97/399 (24.31%), Postives = 166/399 (41.60%), Query Frame = 1 
Query:  379 ESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFS-----PVSNENKVECLDTPMIFKVMESMDVLNFVERN-----PKNQLSNMKTILSEARNIFQCYDKIRNNH-PKRKRYLIFVTCSDLLLDDI------------------NDLVKDMKSMKVRFSLFSPVKYDSHTNICDLLN-YNSKGCKDLSSYYQSSKWSVIL--------------THGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENS------LNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNS 1425
            ++V  Y++TL+P D +I +E  + F+  T +M  F++     IL  LNGG PL  DFGRD +TSLYSL+ F      P +   K    DTP I+++++  D +   E++     P+ + +    +L  A   F+  DK+R    P   R+LI +  S + L  +                  + ++ +++  KV  S+FSPV   S   + DL+N    K  KD       S+W  ++               HG++ +       P    +   + N  +  S     ++ N + A    Q S +++ H   ++  S       N SL A SL ++       G ++I      L+G   +T   + S S          P YQ  ++ Q     +  
Sbjct:   51 KTVREYVATLRPVDCVIMVEKCRPFNSKTEHMEYFKNNCLQPILDCLNGGPPLDADFGRDAYTSLYSLHDFDWRHPFPATTLAK----DTPKIYRILQCFDSIRHSEKHAPEGTPEFRGTPAIPLLRAALQGFEAIDKVRRGLCPVVFRHLILLALSPVALGPLKAEATALSTGAAVIEEGDSGVLAELRKRKVAVSVFSPVSLPSLRRLADLVNTLPPKPRKD-------SRWPTMVFSRELADWASRYEKRHGMAPS-----PPPCQTSRRPTDSNNNSITSPLSASVLQNGICARTNFQPSVSLTHHLSNEATLSPQQYRQTNHSLPAPSLNSS-------GPNSIEQAPGNLIGPDMATTPGNFSVSPADVGGGGGFPIYQQSSLPQPSPRSIGG 426
[Close]
BLAST of SMED30006127 vs. TrEMBL
Match: A0A183T9P8 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS13196 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 7.525e-19
Identity = 70/243 (28.81%), Postives = 117/243 (48.15%), Query Frame = 1 
Query:  364 RRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFS-----PVSNENKVECLDTPMIFKVMESMDVLNFVERN-----PKNQLSNMKTILSEARNIFQCYDKIRNNH-PKRKRYLIFVTCSDLLLDDI------------------NDLVKDMKSMKVRFSLFSPVKYDSHTNICDLLN-YNSKGCKDLSSYYQSSKWSVIL 1002
            R ++ ++V  Y++TL+P D +I +E  + F+  T +M  F++     IL  LNGG PL  DFGRD +TSLYSL+ F      P +   K    DTP I+++++  D +   E++     P+ + +    +L  A   F+  DK+R    P   R+LI +  S + L  +                  + ++ +++  KV  S+FSPV   S   + DL+N    K  KD       S+W  ++
Sbjct:  393 RLTFFKTVREYVATLRPVDCVIMVEKCRPFNSKTEHMEYFKNNCLQPILDCLNGGPPLDADFGRDAYTSLYSLHDFDWRHPFPATTLAK----DTPKIYRILQCFDSIRHSEKHAPEGTPEFRGTPAIPLLRAALQGFEAIDKVRRGLCPVVFRHLILLALSPVALGPLKAEATALSTGAAVIEEGDSGVLAELRKRKVAVSVFSPVSLPSLRRLADLVNTLPPKPRKD-------SRWPTMV 624
[Close]
BLAST of SMED30006127 vs. TrEMBL
Match: A0A5J4NTM0 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0007463 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 5.840e-18
Identity = 66/208 (31.73%), Postives = 107/208 (51.44%), Query Frame = 1 
Query:  379 ESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSF---SPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQ-----LSNMKTILSEARNIFQCYDKIRNNHPKR-KRYLIFVTCSDLLLDDIN-DLVKD------------MKSMKVRFSLFSPVKYDSHTNICDLLN 936
            E++ SY + L P D +I +E  + F+  T++M  FR  Y   IL  LNGG PL  DFGRD +TSLYSL+ F    P    + V   DTP+I+++++ +  L   ER+   Q      ++ + +L  A   F   D+ R    K+ +R+L+ ++ S + L D++ +LV              ++   V  S FSPV+  S   + +L+N
Sbjct:  397 EAMRSYAAELCPVDCVILVERCRPFNCDTLFMRYFREQYLCPILSNLNGGPPLNADFGRDAYTSLYSLHGFYWRKPHPLTSWVP--DTPIIYRILKRITTLQHEERHASEQSDVLVGTSGRELLESANEAFDAIDRARRGLCKQVQRHLLLLSVSPINLTDLHKELVSPVENRPVTGPLTVLRQRGVALSAFSPVRSPSLLQLYELVN 602
[Close]
BLAST of SMED30006127 vs. TrEMBL
Match: G7YPZ0 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_106481 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.653e-17
Identity = 65/209 (31.10%), Postives = 107/209 (51.20%), Query Frame = 1 
Query:  379 ESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSF---SPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKT------ILSEARNIFQCYDKIRNNHPKR-KRYLIFVTCSDLLLDDIN-DLVK------------DMKSMKVRFSLFSPVKYDSHTNICDLLN 936
            E++ SY + L P D +I +E  + F+  T++M  FR  Y   IL  LNGG PL  DFGRD +TSLYSL+ F    P      V   DTP+I+++++ +  +   ER+  N+ +++ T      +L  A   F   D+ R    K+ +R+LI +T S + L +++ +LV             +++   V  S F+PV   S   + + +N
Sbjct:   83 EAMRSYAAELCPVDCVILMERCRPFNCDTLFMKYFREQYLCPILSNLNGGPPLNADFGRDAYTSLYSLHGFYWRKPRQINTWVP--DTPIIYRILKQIATVKHEERHATNEHTDVFTGTPGHDLLEAAVEAFDAIDRARRGLCKQVQRHLILLTVSPINLAEVHKELVSSSDDCTKSGPLTNLRRRGVALSAFAPVPSPSVLQLYETIN 289
[Close]
BLAST of SMED30006127 vs. TrEMBL
Match: A0A419PLV6 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CSKR_4628s PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.683e-17
Identity = 65/209 (31.10%), Postives = 107/209 (51.20%), Query Frame = 1 
Query:  379 ESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSF---SPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKT------ILSEARNIFQCYDKIRNNHPKR-KRYLIFVTCSDLLLDDIN-DLVK------------DMKSMKVRFSLFSPVKYDSHTNICDLLN 936
            E++ SY + L P D +I +E  + F+  T++M  FR  Y   IL  LNGG PL  DFGRD +TSLYSL+ F    P      V   DTP+I+++++ +  +   ER+  N+ +++ T      +L  A   F   D+ R    K+ +R+LI +T S + L +++ +LV             +++   V  S F+PV   S   + + +N
Sbjct:   83 EAMRSYAAELCPVDCVILMERCRPFNCDTLFMKYFREQYLCPILSNLNGGPPLNADFGRDAYTSLYSLHGFYWRKPRQINTWVP--DTPIIYRILKQIATVKHEERHATNEHTDVFTGTPGHDLLEAAVEAFDAIDRARRGLCKQVQRHLILLTVSPINLAEVHKELVSSSDDCTKSGPLTNLRRRGVALSAFAPVPSPSVLQLYETIN 289
[Close]
BLAST of SMED30006127 vs. Planmine SMEST
Match: SMESG000018155.1 (SMESG000018155.1)

HSP 1 Score: 1498.41 bits (3878), Expect = 0.000e+0
Identity = 791/829 (95.42%), Postives = 791/829 (95.42%), Query Frame = 1 
Query:  196 MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLEXXXXXXXXXXXDLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLVKDMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYXXXXXXXXXXXXXXXXXXXXXQSPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQXXXXXXXXXXXXXXXXRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINLRFXXXXXXXXXXXXXXXXXXXFSMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY 2682
            MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLENSINSKSNNSSDLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLV DMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYSAPNSVS               SPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQNNIINPNNNNNNMNPNRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINL                      SMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY
Sbjct:    1 MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLENSINSKSNNSSDLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLVNDMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYSAPNSVS---------------SPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQNNIINPNNNNNNMNPNRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINL----------------------SMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY 792
[Close]
BLAST of SMED30006127 vs. Planmine SMEST
Match: SMESG000018155.1 (SMESG000018155.1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.000e+0
Identity = 786/831 (94.58%), Postives = 790/831 (95.07%), Query Frame = 1 
Query:  196 MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLEXXXXXXXXXXX--DLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLVKDMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYXXXXXXXXXXXXXXXXXXXXXQSPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQXXXXXXXXXXXXXXXXRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINLRFXXXXXXXXXXXXXXXXXXXFSMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY 2682
            MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLENSINS+S +++  DLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLV DMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYSAPNSVS               SPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQNNIINPNNNNNNMNPNRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINL                      SMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY
Sbjct:    1 MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLENSINSESKSNNSSDLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYYFDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIFKVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRYLIFVTCSDLLLDDINDLVNDMKSMKVRFSLFSPVKYDSHTNICDLLNYNSKGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACDSTEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQLEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAINQSQINKVNSMPCYYYPDQLYSAPNSVS---------------SPQFHMGNTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSFQRFSYAYNSQNNIINPNNNNNNMNPNRFVIQPVYKDPRNNSITLFEGYLIYFEDKRADIVLCIDQNINL----------------------SMFSMDSWDRQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIKTICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPADPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTMPRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYSSLPNQSANHMIQMDMGLQSNQNIFDQNNY 794
The following BLAST results are available for this feature:
BLAST of SMED30006127 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0V0J9X92.070e-1924.31Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183T9P87.525e-1928.81Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A5J4NTM05.840e-1831.73Uncharacterized protein OS=Paragonimus westermani ... [more]
G7YPZ04.653e-1731.10Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A419PLV65.683e-1731.10Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
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BLAST of SMED30006127 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30006127 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000018155.10.000e+095.42SMESG000018155.1[more]
SMESG000018155.10.000e+094.58SMESG000018155.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006127 ID=SMED30006127|Name=SMED30006127|organism=Schmidtea mediterranea sexual|type=transcript|length=2753bp
TATTAATTTTTCTTGTTTAATTGTATGATATTACTTATCGCAATTTTCAA
ACAAATCATTATTTTATAATTAGTAATTTATTGATAATTTCTCAAAATTC
AAAATTTTATTTTATATATTCGCAATTGTTAATCATATTTTTAATCCTAA
AAGTAACTAGTATTTTTCAGGAACTTATATTTGTAATTATTTAAAATGAC
AACTGAAAATCCTTCTATTGCTGAAGATACTAAATTTATTCAACCTTCAG
AATCTCAAACATTAGATTCTCTTCTTCAGAACTCATGTGAAGCATTAACT
CAGTCTGAAACATTAGAAAATTCAATCAACAGTAAATCTAATAACAGTAG
TGATTTATTTCCTAGAAGGTCTTGGAATGAAAGTGTGGAATCATATATTA
GTACATTGAAACCAAAGGATACGTTGATATTTATAGAAAATATTAAAGCA
TTTAGTAAAGGAACCGTTTACATGTCTGATTTTAGAAGTTATTATTTCGA
TGTTATTTTGAAATTTTTAAATGGCGGTGAGCCTCTTAAAACGGACTTTG
GACGTGATGAGTTTACATCATTGTATTCTTTGTATTCATTTTCACCCGTT
AGTAATGAAAATAAAGTTGAATGCCTAGACACCCCCATGATATTCAAAGT
AATGGAAAGTATGGATGTTTTGAATTTCGTTGAAAGAAATCCAAAAAACC
AATTATCAAATATGAAAACTATATTGAGTGAAGCAAGAAATATTTTTCAA
TGTTATGATAAAATTAGAAACAACCATCCTAAAAGAAAGAGATATTTAAT
TTTTGTTACTTGTTCTGATTTGTTGCTAGATGACATTAATGATTTGGTAA
AGGACATGAAATCAATGAAAGTTCGTTTTAGTTTATTCAGTCCTGTTAAA
TATGATTCTCACACGAATATATGCGATTTGTTGAATTACAACAGTAAAGG
TTGTAAAGACTTGTCTTCTTATTACCAATCATCGAAATGGTCTGTTATTT
TAACCCATGGTTTAAGCAAAGCTTTCGAATCTGCTAATTCATGTCCGATA
CCGAATAAAAAAATGAAATGGAACCAGAATAAAGAAGCCTGTGATTCTAC
GGAATATGATTTTATTATGATGAATTTAGTAAATGCGTCCAACTGTGTTC
AGAATTCAGAAAATATATCAACTCATGCCCAAGCGGATAGTGAAAATTCC
CTCAACACGAGTCTCAATGCAAGATCATTGACAAATAATGAGCAACTGGA
ACAAAATTATGGCCAATCAAATATCACATATAACAACAGCACATTGGTTG
GAAGTTCCCAGTCTACTCTCACTCATTCAAAGAGCCAATCAAAATTCATG
CAATCTACTAATAATAATACCCCTTATTACCAATCCGGCGCTATAAATCA
ATCCCAGATCAACAAAGTCAATTCGATGCCATGCTATTATTATCCAGATC
AGCTGTATTCGGCTCCTAATAGTGTCAGTGTTAGTTCTCATAGATCGACC
CCTTCGTCCATTCCCCACTCGCAGTCACCTCAATTTCACATGGGAAATAC
ACATTCAAATTTAGTTCAGCAAAGCAATTCGGTTTCGGAAGTTATTAAGC
CAAGCAGTGTCGAATCGTGCGGTGAAATTCACGGCCATGTAAATTCTCAA
AAATCCCTCCAAATGAATTCAGATGTGGAAATGGTTCCGTCATTTCAAAG
ATTTTCCTACGCCTACAATTCACAAAACAACATAATAAATCCCAACAACA
ACAATAACAACATGAACCCAAACCGATTTGTTATCCAACCGGTTTATAAA
GATCCCAGAAATAATTCAATCACATTATTTGAAGGATATCTCATTTATTT
CGAAGACAAAAGAGCTGATATTGTTTTGTGTATCGATCAAAACATTAATT
TAAGGTTTTATATAATTATCCTATATCTCTATCTCTCTCTATATCTATCT
ATATATATATATATATTTAGCATGTTTTCTATGGATTCCTGGGATCGTCA
GTGCTCTATTTACTATGTCAATTACTTTGATTCTTATGGCCCTAGTTTAT
ATGGTAAAAATTCAGATATTGAAGTAATTAGTAAAGAGAGCACATGTAAA
ACATCAGTTGAATTGCAAATCATAATGAATTCCGAAGCTATAAAGACAAT
TTGTAATACGGTTGCATCAAAGCAGAACTACACCATGGCAATAGTCAGAC
CGAGTCGATGCATTCCACCCGATGTCCATGTGCAGATTGTTTGTTTTATT
TTCTCCAAATCTCTCTCAACTAACTTTAAAGTAATAATTCCCGCTGATCC
TAAGAATCTACTCAATGAAATTAAAAGCAGAATGGGAACAATGTCGAACG
CGACTTACTCCAATCAACCGAATCAAATATATTCTTCAATGAAAAGAGGG
AACCTCCCTAATCAAATTTACAATATCCCTCCAGCAATGACAATGCCGAG
GCAAATCATCAATCGGTATAATTCCCATAACAATTTGCCCATGATGGCCA
ATCAGGTGTATAGAAATCCCCATCCTTCTAATTTTATAAATAACCAACAA
CTGCCGGTTAATTCCAATTATATCATGCATTCTGGTCAGTATTCCTCACT
TCCCAATCAGTCTGCAAATCACATGATTCAAATGGATATGGGGTTGCAAT
CTAATCAAAATATCTTTGATCAAAATAATTACTGATTTCAACACGAGTTC
TTGTTTATATTGAAGGATAACACTTGATTTAGTTCTTTTGTGTAGAATAA
AAT
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protein sequence of SMED30006127-orf-1

>SMED30006127-orf-1 ID=SMED30006127-orf-1|Name=SMED30006127-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=830bp
MTTENPSIAEDTKFIQPSESQTLDSLLQNSCEALTQSETLENSINSKSNN
SSDLFPRRSWNESVESYISTLKPKDTLIFIENIKAFSKGTVYMSDFRSYY
FDVILKFLNGGEPLKTDFGRDEFTSLYSLYSFSPVSNENKVECLDTPMIF
KVMESMDVLNFVERNPKNQLSNMKTILSEARNIFQCYDKIRNNHPKRKRY
LIFVTCSDLLLDDINDLVKDMKSMKVRFSLFSPVKYDSHTNICDLLNYNS
KGCKDLSSYYQSSKWSVILTHGLSKAFESANSCPIPNKKMKWNQNKEACD
STEYDFIMMNLVNASNCVQNSENISTHAQADSENSLNTSLNARSLTNNEQ
LEQNYGQSNITYNNSTLVGSSQSTLTHSKSQSKFMQSTNNNTPYYQSGAI
NQSQINKVNSMPCYYYPDQLYSAPNSVSVSSHRSTPSSIPHSQSPQFHMG
NTHSNLVQQSNSVSEVIKPSSVESCGEIHGHVNSQKSLQMNSDVEMVPSF
QRFSYAYNSQNNIINPNNNNNNMNPNRFVIQPVYKDPRNNSITLFEGYLI
YFEDKRADIVLCIDQNINLRFYIIILYLYLSLYLSIYIYIFSMFSMDSWD
RQCSIYYVNYFDSYGPSLYGKNSDIEVISKESTCKTSVELQIIMNSEAIK
TICNTVASKQNYTMAIVRPSRCIPPDVHVQIVCFIFSKSLSTNFKVIIPA
DPKNLLNEIKSRMGTMSNATYSNQPNQIYSSMKRGNLPNQIYNIPPAMTM
PRQIINRYNSHNNLPMMANQVYRNPHPSNFINNQQLPVNSNYIMHSGQYS
SLPNQSANHMIQMDMGLQSNQNIFDQNNY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR021419Mediator_Med25_VWA