SMED30006051
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30006051 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Alignments
SMED30006051 aligns in the following genomic locations:
Homology
BLAST of SMED30006051 vs. Planmine SMEST
Match: SMESG000010439.1 (SMESG000010439.1) HSP 1 Score: 753.821 bits (1945), Expect = 0.000e+0 Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 2 Query: 95 MGFGDSNNLLVSVIIFVFMATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK 1189 MGFGDSNNLLVSVIIFVFMATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK Sbjct: 1 MGFGDSNNLLVSVIIFVFMATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK 365
BLAST of SMED30006051 vs. Planmine SMEST
Match: SMESG000010439.1 (SMESG000010439.1) HSP 1 Score: 717.998 bits (1852), Expect = 0.000e+0 Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 2 Query: 149 MATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK 1189 MATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK Sbjct: 1 MATSHSIHSNKPMAISVSLFRNLPPDIDRPFNVTVNFSSYHKINSAFIVYLVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFYSMNYKENATNNNGTEIYQSVISCNVSYLVDMKLVFGKSHVNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKK 347
BLAST of SMED30006051 vs. Planmine SMEST
Match: SMESG000066856.1 (SMESG000066856.1) HSP 1 Score: 112.079 bits (279), Expect = 3.347e-26 Identity = 104/395 (26.33%), Postives = 182/395 (46.08%), Query Frame = 2 Query: 194 SVSLFRNLPPDIDRPFNVTVNFSSYH------KINSAFIVY---LVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFY----SMNYKENATNNNGT--EIYQSVISCNVSYLVDMKLVF--GKSH-VNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKKVFFVIPVAIIFLLFLVTVLKVHFCKQ------GKVLQKSKQLDWN 1306 S++L NL D F + VN SS+H K S F++ +V +SE + + PG + ++ I +Y SV++ K + +++ + H A L+ + Y + N + NA N I+ I N+ ++D + G++ VN E ++ I+C + P + + L+ +DQ++V + K + + A + +H+ + C S T R IKI+HHPT+++ P+ A ++P S+ C GYPPP + W+ I GP NAS++Y++ L I P ++W F C A N +P S G +F + G++ + V + AI LL ++ VL + L KSK+++ N Sbjct: 18 SITLCSNLSIDRTDKFPIHVN-SSFHILFKTVKTYSPFLLVKLQIVRRSE----IEETAFINPGIENNLENKISNKYMSVIN-WHKNTYRAIEMIIQFHPPANGLIFEILYFDSETANDRYNAFRNPSVMNRIFSEQIQLNIDAILDFYIATRDGRTDGVNSE-----EMAVISCESK-NGVPPGRPI-LKYIDQLDVKNRKFHEADNRIVVHAFLRKSDHNKYVKCMSTGVTEYRYIKIFHHPTALQLRPNYAG--TMPNGSSVLCTAADGYPPPTINWEFISGPPNASQFYSITAGNN-LTILPHCPLHTQWTFNCTALNSIPKSYDLGTTRSFTFTVSP-GIKVAEIVLGSL-AAIFVLLIIIVVLMKKKSVIKKKMKFSRTLNKSKEINLN 394
BLAST of SMED30006051 vs. Planmine SMEST
Match: SMESG000066856.1 (SMESG000066856.1) HSP 1 Score: 112.079 bits (279), Expect = 3.374e-26 Identity = 104/395 (26.33%), Postives = 182/395 (46.08%), Query Frame = 2 Query: 194 SVSLFRNLPPDIDRPFNVTVNFSSYH------KINSAFIVY---LVNKSETPPKMMQNFIFRPGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFY----SMNYKENATNNNGT--EIYQSVISCNVSYLVDMKLVF--GKSH-VNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNIPELGLEARKKVFFVIPVAIIFLLFLVTVLKVHFCKQ------GKVLQKSKQLDWN 1306 S++L NL D F + VN SS+H K S F++ +V +SE + + PG + ++ I +Y SV++ K + +++ + H A L+ + Y + N + NA N I+ I N+ ++D + G++ VN E ++ I+C + P + + L+ +DQ++V + K + + A + +H+ + C S T R IKI+HHPT+++ P+ A ++P S+ C GYPPP + W+ I GP NAS++Y++ L I P ++W F C A N +P S G +F + G++ + V + AI LL ++ VL + L KSK+++ N Sbjct: 17 SITLCSNLSIDRTDKFPIHVN-SSFHILFKTVKTYSPFLLVKLQIVRRSE----IEETAFINPGIENNLENKISNKYMSVIN-WHKNTYRAIEMIIQFHPPANGLIFEILYFDSETANDRYNAFRNPSVMNRIFSEQIQLNIDAILDFYIATRDGRTDGVNSE-----EMAVISCESK-NGVPPGRPI-LKYIDQLDVKNRKFHEADNRIVVHAFLRKSDHNKYVKCMSTGVTEYRYIKIFHHPTALQLRPNYAG--TMPNGSSVLCTAADGYPPPTINWEFISGPPNASQFYSITAGNN-LTILPHCPLHTQWTFNCTALNSIPKSYDLGTTRSFTFTVSP-GIKVAEIVLGSL-AAIFVLLIIIVVLMKKKSVIKKKMKFSRTLNKSKEINLN 393
BLAST of SMED30006051 vs. Planmine SMEST
Match: SMESG000002213.1 (SMESG000002213.1) HSP 1 Score: 103.605 bits (257), Expect = 6.169e-24 Identity = 75/276 (27.17%), Postives = 130/276 (47.10%), Query Frame = 2 Query: 353 PGSQTKIKGFIIGEYFSVVHCLSKTEEKQLKLDLILHEKAQNLVLLMFY--SMNYKENATNNNG--TEIYQSVISCNVSYLVDMKLVF--GKSH-VNEEAFPPNQLIWINCSASIEHQPYWKSVRLENLDQMEVFDVKINNRTKETSILAEITLKNHDSLIVCRSVNSTISRRIKIYHHPTSVKTSPSVASRSSIPYNVSITCVTTGGYPPPIVEWKLIIGPLNASRYYTLNKNKTVLVITKKAPPGSKWIFQCFAKNKLPWSEFSGPEMIVSFNI 1159 PG + +K I +Y SV++ K + +++ + H A L+ + Y + NA N I+ I N+ ++D + G++ VN E ++ I+C S P + + L+ +DQ++V + K + + A + +H+ + C S T R IKI+HHPT+++ P+ A ++P S+ C GYPPP + W+ I GP NAS++Y++ L I P ++W F C A N +P S G +F + Sbjct: 73 PGIENNLKNKISNKYMSVIN-WHKNTYRAIEMIIQFHPSANRLIFEILYYNGTGNRYNAFRNRSVMNRIFSEQIQLNIDAILDFYIATRDGRTDGVNSE-----EMAVISCE-SKNGVPPGRPI-LKYIDQLDVKNRKFHEADNRIVVHAFLRKSDHNKYVKCMSTGVTEYRYIKIFHHPTALQLRPNYAG--TMPNGSSVLCTAADGYPPPTINWEFISGPPNASQFYSITAGNN-LTILPHCPLHTQWTFNCTALNSIPKSYDLGTTRSFTFTV 337 The following BLAST results are available for this feature:
BLAST of SMED30006051 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30006051 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30006051 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30006051 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30006051 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30006051 ID=SMED30006051|Name=SMED30006051|organism=Schmidtea mediterranea sexual|type=transcript|length=1699bpback to top protein sequence of SMED30006051-orf-1 >SMED30006051-orf-1 ID=SMED30006051-orf-1|Name=SMED30006051-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=490bp MGFGDSNNLLVSVIIFVFMATSHSIHSNKPMAISVSLFRNLPPDIDRPFNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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