smoothened-like protein

Overview
Namesmoothened-like protein (ACZ64512.1)
Smed IDSMED30006045
Length (bp)3446
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of smoothened-like protein (SMED30006045) t-SNE clustered cells

Violin plots show distribution of expression levels for smoothened-like protein (SMED30006045) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of smoothened-like protein (SMED30006045) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for smoothened-like protein (SMED30006045) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30006045

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30006045SMESG000040116.1 dd_Smed_v4_9474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
esophagusSMED30006045SMESG000040116.1 GQ337476smed_ncbi_20200123PMID:19933103
Rink et al., 2009		whole organism asexual adult colorimetric in situ hybridization evidence
central nervous systemSMED30006045SMESG000040116.1 GQ337476smed_ncbi_20200123PMID:19933103
Rink et al., 2009		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of smoothened-like protein vs. Ensembl Human
Match: SMO (smoothened, frizzled class receptor [Source:HGNC Symbol;Acc:HGNC:11119])

HSP 1 Score: 80.8777 bits (198), Expect = 1.015e-14
Identity = 70/282 (24.82%), Postives = 119/282 (42.20%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P R +C+ T+  C+I                          VE+    P FL C  +++P+ C  +   +K N   QC++P V T     W + ++GC   C+ P F+  E+    S     G +  L                 V  W        +I  Y+       SIGW+ QF+    + IVCR+DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:  109 WSGLRNAPRCWAVIQPLLCAVYMPKCENDR----VELPSRTLCQATRGPCAI--------------------------VERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCT--------LFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTS--NETLSCVIIFVIVYYALMAGVVWFVVLTY--AWHTSFKALGT 348
[Close]
BLAST of smoothened-like protein vs. Ensembl Fly
Match: smo (gene:FBgn0003444 transcript:FBtr0078129)

HSP 1 Score: 82.4185 bits (202), Expect = 1.676e-15
Identity = 77/260 (29.62%), Postives = 122/260 (46.92%), Query Frame = 1 
Query:  196 TCFNIKFTF-MYTFPNTNFL-------DINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNK--YPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREP 942
            TCF  K  + + +   T+F         +N + A+K +PKCW  +Q  LC++  P+C+K     M+  P  ++C IT + C IL               +T F          P FL CN+  +P  C   +  +K N   QC  P V T   + +  GI+GC   C+ P +++DE+     L G+ G I  L NL V+ TF       + Y      + +F I++  +I+ +GW++QF     + IVCR DG ++  EP
Sbjct:   99 TCFGSKLPYELSSLDLTDFHTEKELNDKLNDYYALKHVPKCWAAIQPFLCAVFKPKCEKINGEDMVYLPSYEMCRITMEPCRIL--------------YNTTF---------FPKFLRCNETLFPTKCTNGARGMKFNGTGQCLSPLVPTDTSASYYPGIEGCGVRCKDPLYTDDEHRQIHKLIGWAGSICLLSNLFVVSTFFIDWKNANKYP----AVIVFYINLCFLIACVGWLLQFTSGSREDIVCRKDGTLRHSEP 331
[Close]
BLAST of smoothened-like protein vs. Ensembl Zebrafish
Match: smo (smoothened, frizzled class receptor [Source:ZFIN;Acc:ZDB-GENE-980526-89])

HSP 1 Score: 83.5741 bits (205), Expect = 1.010e-15
Identity = 76/289 (26.30%), Postives = 123/289 (42.56%), Query Frame = 1 
Query:  247 FLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDCN---KYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            F  +  WS +++ P+CW  +Q LLC++  P+C+  K    +  P + +C+ T+  CSI                          VE+    P+FL C    ++P+ C  +  KLK N   QC+ P V T  ++ W K ++GC   C  P F+ DE++   S     G I  L          F      +  W        +I  Y+       SIGW+ QF+      IVC+SD  +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   81 FEKLAMWSGLRNAPRCWAVIQPLLCAVYMPKCENGK----VELPSQHLCQATRNPCSI--------------------------VERERGWPNFLKCENKEQFPKGCQNEVQKLKFNTSGQCEAPLVKTDIQASWYKDVEGCGIQCDNPLFTEDEHSDMHSYIAVFGTITLLCT--------FFTLATFLADWKNSNRYPAVILFYVNACFFIGSIGWLAQFMDGARNEIVCKSDNTMRLGEPSS--TETLSCVIIFVIVYYSLMSGVIWFVMLTY--AWHTSFKALGT 327
[Close]
BLAST of smoothened-like protein vs. Ensembl Xenopus
Match: SMO (smoothened, frizzled class receptor [Source:NCBI gene;Acc:100491644])

HSP 1 Score: 72.4034 bits (176), Expect = 3.587e-12
Identity = 66/275 (24.00%), Postives = 118/275 (42.91%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  ++ LLC++  P+C+  K    +  P + +C+ T+  C+I+                       +     P FL C  + +P  C  +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+ I   +       T             +P  + LF ++    + SIGW+ QF+      IVCR DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   82 WSGLRNAPRCWDAIRPLLCAVYMPKCEGGK----VELPSQSLCQTTRVPCAIV-----------------------ARERGWPDFLKCTTDHFPDGCSNEIQTVKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTKKEH---REMHVYIAIFSSVTIFCTFFTLATFLSDWKNSNRYP-AVILFYVNACFFVGSIGWLAQFMDGARDEIVCRGDGTMRLGEPTS--NETLSCVIIFVIVYYAMMSGVIWFVMLTY--AWHTSFKALGT 321
[Close]
BLAST of smoothened-like protein vs. Ensembl Xenopus
Match: SMO (smoothened, frizzled class receptor [Source:NCBI gene;Acc:100491644])

HSP 1 Score: 72.4034 bits (176), Expect = 3.703e-12
Identity = 66/275 (24.00%), Postives = 118/275 (42.91%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  ++ LLC++  P+C+  K    +  P + +C+ T+  C+I+                       +     P FL C  + +P  C  +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+ I   +       T             +P  + LF ++    + SIGW+ QF+      IVCR DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   82 WSGLRNAPRCWDAIRPLLCAVYMPKCEGGK----VELPSQSLCQTTRVPCAIV-----------------------ARERGWPDFLKCTTDHFPDGCSNEIQTVKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTKKEH---REMHVYIAIFSSVTIFCTFFTLATFLSDWKNSNRYP-AVILFYVNACFFVGSIGWLAQFMDGARDEIVCRGDGTMRLGEPTS--NETLSCVIIFVIVYYAMMSGVIWFVMLTY--AWHTSFKALGT 321
[Close]
BLAST of smoothened-like protein vs. Ensembl Xenopus
Match: SMO (smoothened, frizzled class receptor [Source:NCBI gene;Acc:100491644])

HSP 1 Score: 72.0182 bits (175), Expect = 4.130e-12
Identity = 66/275 (24.00%), Postives = 118/275 (42.91%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  ++ LLC++  P+C+  K    +  P + +C+ T+  C+I+                       +     P FL C  + +P  C  +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+ I   +       T             +P  + LF ++    + SIGW+ QF+      IVCR DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   82 WSGLRNAPRCWDAIRPLLCAVYMPKCEGGK----VELPSQSLCQTTRVPCAIV-----------------------ARERGWPDFLKCTTDHFPDGCSNEIQTVKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTKKEH---REMHVYIAIFSSVTIFCTFFTLATFLSDWKNSNRYP-AVILFYVNACFFVGSIGWLAQFMDGARDEIVCRGDGTMRLGEPTS--NETLSCVIIFVIVYYAMMSGVIWFVMLTY--AWHTSFKALGT 321
[Close]
BLAST of smoothened-like protein vs. Ensembl Xenopus
Match: SMO (smoothened, frizzled class receptor [Source:NCBI gene;Acc:100491644])

HSP 1 Score: 72.0182 bits (175), Expect = 4.692e-12
Identity = 66/275 (24.00%), Postives = 118/275 (42.91%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  ++ LLC++  P+C+  K    +  P + +C+ T+  C+I+                       +     P FL C  + +P  C  +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+ I   +       T             +P  + LF ++    + SIGW+ QF+      IVCR DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   82 WSGLRNAPRCWDAIRPLLCAVYMPKCEGGK----VELPSQSLCQTTRVPCAIV-----------------------ARERGWPDFLKCTTDHFPDGCSNEIQTVKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTKKEH---REMHVYIAIFSSVTIFCTFFTLATFLSDWKNSNRYP-AVILFYVNACFFVGSIGWLAQFMDGARDEIVCRGDGTMRLGEPTS--NETLSCVIIFVIVYYAMMSGVIWFVMLTY--AWHTSFKALGT 321
[Close]
BLAST of smoothened-like protein vs. Ensembl Xenopus
Match: SMO (smoothened, frizzled class receptor [Source:NCBI gene;Acc:100491644])

HSP 1 Score: 72.0182 bits (175), Expect = 5.025e-12
Identity = 66/275 (24.00%), Postives = 118/275 (42.91%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  ++ LLC++  P+C+  K    +  P + +C+ T+  C+I+                       +     P FL C  + +P  C  +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+ I   +       T             +P  + LF ++    + SIGW+ QF+      IVCR DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   68 WSGLRNAPRCWDAIRPLLCAVYMPKCEGGK----VELPSQSLCQTTRVPCAIV-----------------------ARERGWPDFLKCTTDHFPDGCSNEIQTVKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTKKEH---REMHVYIAIFSSVTIFCTFFTLATFLSDWKNSNRYP-AVILFYVNACFFVGSIGWLAQFMDGARDEIVCRGDGTMRLGEPTS--NETLSCVIIFVIVYYAMMSGVIWFVMLTY--AWHTSFKALGT 307
[Close]
BLAST of smoothened-like protein vs. Ensembl Mouse
Match: Smo (smoothened, frizzled class receptor [Source:MGI Symbol;Acc:MGI:108075])

HSP 1 Score: 81.6481 bits (200), Expect = 3.389e-15
Identity = 71/282 (25.18%), Postives = 117/282 (41.49%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P R +C+ T+  C+I                          VE+    P FL C  + +P+ CP +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+    S     G +  L                 V  W        +I  Y+       SIGW+ QF+    + IVCR+DG ++  EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:  113 WSGLRNAPRCWAVIQPLLCAVYMPKCENDR----VELPSRTLCQATRGPCAI--------------------------VERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCT--------LFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTS--SETLSCVIIFVIVYYALMAGVVWFVVLTY--AWHTSFKALGT 352
[Close]
BLAST of smoothened-like protein vs. UniProt/SwissProt
Match: sp|P91682|SMO_DROME (Protein smoothened OS=Drosophila melanogaster OX=7227 GN=smo PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.680e-14
Identity = 77/260 (29.62%), Postives = 122/260 (46.92%), Query Frame = 1 
Query:  196 TCFNIKFTF-MYTFPNTNFL-------DINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNK--YPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREP 942
            TCF  K  + + +   T+F         +N + A+K +PKCW  +Q  LC++  P+C+K     M+  P  ++C IT + C IL               +T F          P FL CN+  +P  C   +  +K N   QC  P V T   + +  GI+GC   C+ P +++DE+     L G+ G I  L NL V+ TF       + Y      + +F I++  +I+ +GW++QF     + IVCR DG ++  EP
Sbjct:   99 TCFGSKLPYELSSLDLTDFHTEKELNDKLNDYYALKHVPKCWAAIQPFLCAVFKPKCEKINGEDMVYLPSYEMCRITMEPCRIL--------------YNTTF---------FPKFLRCNETLFPTKCTNGARGMKFNGTGQCLSPLVPTDTSASYYPGIEGCGVRCKDPLYTDDEHRQIHKLIGWAGSICLLSNLFVVSTFFIDWKNANKYP----AVIVFYINLCFLIACVGWLLQFTSGSREDIVCRKDGTLRHSEP 331
[Close]
BLAST of smoothened-like protein vs. UniProt/SwissProt
Match: sp|P56726|SMO_MOUSE (Smoothened homolog OS=Mus musculus OX=10090 GN=Smo PE=1 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 2.371e-14
Identity = 71/282 (25.18%), Postives = 117/282 (41.49%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P R +C+ T+  C+I                          VE+    P FL C  + +P+ CP +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+    S     G +  L                 V  W        +I  Y+       SIGW+ QF+    + IVCR+DG ++  EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:  113 WSGLRNAPRCWAVIQPLLCAVYMPKCENDR----VELPSRTLCQATRGPCAI--------------------------VERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCT--------LFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTS--SETLSCVIIFVIVYYALMAGVVWFVVLTY--AWHTSFKALGT 352
[Close]
BLAST of smoothened-like protein vs. UniProt/SwissProt
Match: sp|P97698|SMO_RAT (Smoothened homolog OS=Rattus norvegicus OX=10116 GN=Smo PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.517e-14
Identity = 71/282 (25.18%), Postives = 117/282 (41.49%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P R +C+ T+  C+I                          VE+    P FL C  + +P+ CP +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+    S     G +  L                 V  W        +I  Y+       SIGW+ QF+    + IVCR+DG ++  EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:  113 WSGLRNAPRCWAVIQPLLCAVYMPKCENDR----VELPSRTLCQATRGPCAI--------------------------VERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCT--------LFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTS--SETLSCVIIFVIVYYALMAGVVWFVVLTY--AWHTSFKALGT 352
[Close]
BLAST of smoothened-like protein vs. UniProt/SwissProt
Match: sp|Q99835|SMO_HUMAN (Smoothened homolog OS=Homo sapiens OX=9606 GN=SMO PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.873e-14
Identity = 70/282 (24.82%), Postives = 119/282 (42.20%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P R +C+ T+  C+I                          VE+    P FL C  +++P+ C  +   +K N   QC++P V T     W + ++GC   C+ P F+  E+    S     G +  L                 V  W        +I  Y+       SIGW+ QF+    + IVCR+DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:  109 WSGLRNAPRCWAVIQPLLCAVYMPKCENDR----VELPSRTLCQATRGPCAI--------------------------VERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCT--------LFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTS--NETLSCVIIFVIVYYALMAGVVWFVVLTY--AWHTSFKALGT 348
[Close]
BLAST of smoothened-like protein vs. UniProt/SwissProt
Match: sp|O42224|SMO_CHICK (Smoothened homolog (Fragment) OS=Gallus gallus OX=9031 GN=SMO PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.303e-12
Identity = 68/282 (24.11%), Postives = 120/282 (42.55%), Query Frame = 1 
Query:  265 WSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            WS +++ P+CW  +Q LLC++  P+C+  +    +  P + +C+ T+  C+I                          VE+    P FL C  +++P+ CP +   +K N   QC+ P V T     W + ++GC   C+ P F+  E+   + +  Y+      ++        F      V  W        +I  Y+       SIG + QF+      IVCR+DG +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   79 WSGLRNAPRCWDVIQPLLCAVYMPKCEDGQ----VELPSQTLCQATRAPCTI--------------------------VERERGWPDFLKCTPDRFPEGCPNEVQNIKFNSSGQCEAPLVRTYNPKSWYEDVEGCGIQCQNPLFTETEH---REMHVYIAFSSVTISCT------FFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGCVAQFMDGARDEIVCRADGTMRLGEPTS--NETLSCVIIFVIVYYSLMSGVIWFVMLTY--AWHTSFKALGT 317
[Close]
BLAST of smoothened-like protein vs. TrEMBL
Match: D2ILS1 (Smoothened-like protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 1363.98 bits (3529), Expect = 0.000e+0
Identity = 802/811 (98.89%), Postives = 806/811 (99.38%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH-KKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFXXXXXXXXXXXXXXGIDKESGEFCSDDEVMSLINSENH 3352
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH +KSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH
Sbjct:  305 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHNRKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH 1115

HSP 2 Score: 516.538 bits (1329), Expect = 1.482e-160
Identity = 292/295 (98.98%), Postives = 292/295 (98.98%), Query Frame = 1 
Query:   64 CVSSQFVNYSIFRNNFAMNYSYS-REGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            CVSSQFVNYSIFRNNFAMNYSY  REGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:   16 CVSSQFVNYSIFRNNFAMNYSYIFREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 310
[Close]
BLAST of smoothened-like protein vs. TrEMBL
Match: E2FMT3 (Smoothened OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.698e-17
Identity = 82/283 (28.98%), Postives = 123/283 (43.46%), Query Frame = 1 
Query:  142 CAEPVSECQLLNNVLENSTCFNIKFTFMYTF------PNTN---FLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT--PSFLDCNK--YPQNCPADSFKLK--NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            C  PV+ CQ     L N TC   K  + +T        +T    F ++  WS ++++P+CW  +Q  LC +  P C   K    ++ P +++CE T++ C                           VVEK   P FL C++  +   C +   KL+      +C+ P V T  +  W + +DGC   C+ P F++ E+N    + G    +  +     L+TF       S Y      L LF I+    I SIGW  QFIP     IVCR DG V+ +EPQ
Sbjct:  174 CTRPVTSCQ----PLVNQTCLGAKLPYTHTSIEMVGDASTQLEVFNNLRLWSGLRNVPRCWDVIQPFLCGIYLPRCVGSK----VDLPSQEMCERTREPCK--------------------------VVEKKGWPDFLKCDQPHFISGCDSQVTKLRFNTSSGRCESPLVKTANKGSWYQDVDGCGIQCQNPLFTDSEHNEVHHIIGTFAALCMIATAFALLTFIIDWRNASRYP----SLILFFINACFFIGSIGWAAQFIPGARDEIVCRKDGTVRTQEPQ 418
[Close]
BLAST of smoothened-like protein vs. TrEMBL
Match: T1ISZ0 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 8.673e-17
Identity = 77/276 (27.90%), Postives = 127/276 (46.01%), Query Frame = 1 
Query:  256 INYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNK--YPQNCPAD--SFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTF 1071
            +  W+ + S+P+CW  ++ LLC++  P C+       I+ P   +C IT++ C I+                        V+E  P FL C+K  +   C  +    K  +   +C  P V +     WI G++GC  PC  P F++ E++  +S    VG I    +L  ++T  F     SV K+  +   +F I+   +I +IGWM QF P   + IVCR DG +++REP +    + C      I+F++   F    V  F F+ Y  + +F
Sbjct:  116 MQLWAGLSSVPRCWSVIRPLLCAVYMPRCENGS----IDLPSYDLCRITRKRCRIV-----------------------EVIENWPEFLKCDKPHFKHQCKNEWREVKFNSSTARCAAPLVNSDHELSWIDGVEGCGLPCNNPLFTDAEHHHLRSFIIIVGSICLSCSLFAVVT--FLIDWKSVNKYPGL--IIFYINFCFLIVNIGWMAQFTPGAREDIVCRKDGTLRLREPSSG-ENLSCV-----IIFIMVYYFVMAAVIWFVFLTYSWLMSF 354
[Close]
BLAST of smoothened-like protein vs. TrEMBL
Match: R7U0G2 (Uncharacterized protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_108217 PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 8.903e-17
Identity = 81/284 (28.52%), Postives = 129/284 (45.42%), Query Frame = 1 
Query:  133 REGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFP-----NTNFLD----INYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNK--YPQNCPADSFKLK--NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            R  C + V  C   + + + + C  +   + +T       ++N  D    +N W+ ++++PKCW+ +Q LLCS+  P+C +  +  ++  P ++ C+IT+  C I+                     SY      P+FL+CN+  +   C +   K    N    C+ P V T  R+ W K +DGC   C+ P F++ E+          G +     L  L+TF       S Y      L LF I+    I SIGWM QF+P     IVCRSDG +++ EPQ
Sbjct:   15 RPECLQEVEHC---DPIPKPALCLGVSLPYSHTSAILAEDSSNVTDALQKLNLWTGLRNVPKCWEVVQPLLCSVYLPKCIENSNKSVVYLPPKEFCDITRGPCKIV--------------------ESYG----WPAFLNCNQSHFISRCTSTVMKGLSFNTTGTCEAPLVATENRNSWYKDVDGCGVQCKNPLFTDQEHRQMHIFIAVCGSLCLASTLFTLLTFIIDWKNSSRYP----ALILFFINGCFFIGSIGWMAQFVPGTRDDIVCRSDGTMRVAEPQ 267
[Close]
BLAST of smoothened-like protein vs. TrEMBL
Match: A0A0P4WL15 (Uncharacterized protein OS=Scylla olivacea OX=85551 PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.257e-16
Identity = 86/314 (27.39%), Postives = 142/314 (45.22%), Query Frame = 1 
Query:  184 LENSTCFNIKFTFMYT------FPNTNFL--DINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDCN-KYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGSYS 1086
            LE +TC  +   + +T      FPN       +  WSA+++IP+CW  +Q LLC++ +P+C        +  P +++C IT+  C                           VVEK    P FL C+ +YP  C     +LK N   QC  P V T + S W +G+ GC   C  P F+++E+   K L  +V  +  +     + T   +         +P+ L +F I+   +++SIG+M+QF P + Q + CRSDG ++  EP +            +I  V+       FV L+YF+    ++  + +YS
Sbjct:   90 LEETTCLGVTLPYTHTSLALSGFPNQRMAREQLEVWSALRNIPRCWAVVQPLLCAVFFPQCNNSS----VELPSQEMCVITRGPC--------------------------RVVEKEKRWPEFLKCHEEYPHKCKNTVRELKFNTTGQCTPPLVATEEDSSWYEGVKGCGVQCANPIFTDEEH---KDLHSFVATLGGVCLACSIFTVLTYLIDWRAANKYPV-LIIFYINGCFLVASIGFMVQFHPGVRQDLTCRSDGTLREGEPSSG----------GNISCVV------VFVCLYYFMMAGGLWIIILAYS 353
[Close]
BLAST of smoothened-like protein vs. Ensembl Cavefish
Match: smo (smoothened, frizzled class receptor [Source:NCBI gene;Acc:103043901])

HSP 1 Score: 78.9518 bits (193), Expect = 2.245e-14
Identity = 75/286 (26.22%), Postives = 120/286 (41.96%), Query Frame = 1 
Query:  256 INYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKT---PSFLDCN---KYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFTFVFLFYFICYKTIFTFLGS 1080
            +  WS +K+ P+CW  +Q LLC++  P+C+  K    +  P +K+C  T+Q CSI                          VE+    P+FL C     +P  C  +  KLK N    C+ P V T  ++ W K ++GC   C  P F+ +E+    S     G +  L          F      +  W        +I  Y+       SIGW+ QF+    + IVC+SD  +++ EP +   + +   I+  I++   +     FV L Y   + T F  LG+
Sbjct:   90 LAMWSGLKNAPRCWAVIQPLLCAVYMPKCENGK----VELPSQKLCFSTRQPCSI--------------------------VEQERGWPNFLKCENKESFPVGCQNEVQKLKFNTSGHCEAPLVKTDIQASWYKDVEGCGIQCDNPLFTEEEHTDMHSYIAIFGSVTLLCT--------FFTLATFLADWKNSNRYPAVILFYVNACFFIGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSS--TETLSCVIIFIIVYYSLMSGVIWFVMLTY--AWHTSFKALGT 333
[Close]
BLAST of smoothened-like protein vs. Ensembl Sea Lamprey
Match: smo (smoothened, frizzled class receptor [Source:ZFIN;Acc:ZDB-GENE-980526-89])

HSP 1 Score: 85.1149 bits (209), Expect = 3.495e-17
Identity = 60/235 (25.53%), Postives = 110/235 (46.81%), Query Frame = 1 
Query:  253 DINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDC--NKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQN 948
            ++  W+ ++S+P+CW+ +Q LLC++  P C    S   +  P R +C  T+  C+++                TP           P FL C   ++P  CP     +  N    C  P V T   + W++ ++GC  PC +P ++ +E+   ++    +G +  L  +  L TF    +Q S  + +P  + +  I+   +++S GW+ QF+ +  Q I CR DG  ++ EP +
Sbjct:   44 NLQLWAGLRSVPRCWEVVQPLLCAVYMPRC----SAGAVELPSRALCHATRAPCAVVA--------------RTP--------HGWPDFLSCQPERFPDGCPNTVQGVHFNTTSSCAAPLVRTDNPASWVRDVEGCGLPCSSPLYTAEEHAAMRTATAAMGALALLSEIFTLATF-VGDWQRS--RRYP-AVVICYINACFLVASAGWLAQFLSDARQDITCRHDGTPRVGEPTS 248
[Close]
BLAST of smoothened-like protein vs. Ensembl Nematostella
Match: EDO40119 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S829])

HSP 1 Score: 79.337 bits (194), Expect = 4.157e-15
Identity = 80/309 (25.89%), Postives = 133/309 (43.04%), Query Frame = 1 
Query:  184 LENSTCFNIKFTFMYT----FPNTNFLD-----INYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQ-NCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFT----FVFLFYF--ICYKTI 1062
            L+  TC   K  + YT      +++  D     +  W  +K +PKCW+ LQ  LC +  P+C+       +  P R+ C  T++ CS++                     SY    + P FL C+K P+ NC     KL  +   C+ P V T  +  W +GI+GC   C  P FS +E+          G +  +  L  ++TF       + Y      + LF ++    ++S+G+++QF  +  + +VCR D  +++ EP            L  + FV+   F+      FV L Y   IC+KT+
Sbjct:   29 LKEQTCLGEKLDYHYTTTELVTDSSSQDQVKKNLEKWEGLKFLPKCWEVLQPFLCQIYKPKCKNGS----VELPCREQCFATRKPCSVV--------------------ESY---RRWPEFLRCDKLPEGNCNGTLKKLHLNESACKAPLVETAYKPSWYEGIEGCGIQCMNPIFSEEEHTRIHRFVAAFGTLTTVCTLFTVVTFLVGWKSQNRYP----SVILFFMNACFCVASVGFLIQFSSDARRKVVCRRDDTMRLGEPTE------SDSFLCVLTFVLVYYFSLAGMTWFVMLAYSWCICFKTL 300
[Close]
BLAST of smoothened-like protein vs. Ensembl Medaka
Match: smo (smoothened, frizzled class receptor [Source:NCBI gene;Acc:101173992])

HSP 1 Score: 90.5077 bits (223), Expect = 5.528e-18
Identity = 76/288 (26.39%), Postives = 123/288 (42.71%), Query Frame = 1 
Query:  247 FLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLK-NDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXX----XXXGWMMQFIPNLGQSIVCRSDGAVQIREPQNW*IQMVCSRILNDILFVICLKFNFT-----FVFLFYFICYKTIFTFLGS 1080
            F  +  WS +++ P+CW  +Q LLC++  P+C+  +    +  P + +C  T++ CSI+                             P+FL C+++P  C  +  KLK N   QC+ P V T  +S W K ++GC   C  P F+ DE+N   +   Y G I  L          F      +  W        +I  Y+       SIGW+ QF+    + IVC+SD  +++ EP +       S  L+ +   I + ++       FV L Y   + T F  LG+
Sbjct:   80 FEKLTMWSGLRNAPRCWSVIQPLLCAIYMPKCENGQ----VELPSQSLCLATRRPCSIV-----------------------DQERGWPTFLKCDQFPVGCSNEVQKLKFNTSGQCEAPLVKTDIQSSWYKDVEGCGIQCDNPLFTQDEHNDMHAYIAYFGTITLLCT--------FFTLATFLADWKNSNRYPAVILFYINACFFVGSIGWLAQFLDGARKEIVCKSDNTMRLGEPSS-------SETLSCVTIFIIVYYSLMSGVIWFVMLTY--AWHTSFKALGT 323
[Close]
BLAST of smoothened-like protein vs. Planmine SMEST
Match: SMESG000040116.1 (SMESG000040116.1)

HSP 1 Score: 1368.6 bits (3541), Expect = 0.000e+0
Identity = 803/810 (99.14%), Postives = 806/810 (99.51%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFXXXXXXXXXXXXXXGIDKESGEFCSDDEVMSLINSENH 3352
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH
Sbjct:  304 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH 1113

HSP 2 Score: 523.087 bits (1346), Expect = 1.193e-166
Identity = 293/294 (99.66%), Postives = 293/294 (99.66%), Query Frame = 1 
Query:   64 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:   16 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 309
[Close]
BLAST of smoothened-like protein vs. Planmine SMEST
Match: SMESG000040116.1 (SMESG000040116.1)

HSP 1 Score: 1368.21 bits (3540), Expect = 0.000e+0
Identity = 803/810 (99.14%), Postives = 806/810 (99.51%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFXXXXXXXXXXXXXXGIDKESGEFCSDDEVMSLINSENH 3352
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH
Sbjct:  272 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH 1081

HSP 2 Score: 489.574 bits (1259), Expect = 2.834e-154
Identity = 276/277 (99.64%), Postives = 276/277 (99.64%), Query Frame = 1 
Query:  115 MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:    1 MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 277
[Close]
BLAST of smoothened-like protein vs. Planmine SMEST
Match: SMESG000040116.1 (SMESG000040116.1)

HSP 1 Score: 1363.21 bits (3527), Expect = 0.000e+0
Identity = 802/811 (98.89%), Postives = 806/811 (99.38%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH-KKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFXXXXXXXXXXXXXXGIDKESGEFCSDDEVMSLINSENH 3352
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH +KSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH
Sbjct:  272 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHNRKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH 1082

HSP 2 Score: 489.189 bits (1258), Expect = 3.692e-154
Identity = 276/277 (99.64%), Postives = 276/277 (99.64%), Query Frame = 1 
Query:  115 MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:    1 MNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 277
[Close]
BLAST of smoothened-like protein vs. Planmine SMEST
Match: SMESG000040116.1 (SMESG000040116.1)

HSP 1 Score: 1362.82 bits (3526), Expect = 0.000e+0
Identity = 802/811 (98.89%), Postives = 806/811 (99.38%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH-KKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFXXXXXXXXXXXXXXGIDKESGEFCSDDEVMSLINSENH 3352
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVH +KSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH
Sbjct:  304 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHNRKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSDKFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH 1114

HSP 2 Score: 523.087 bits (1346), Expect = 1.089e-166
Identity = 293/294 (99.66%), Postives = 293/294 (99.66%), Query Frame = 1 
Query:   64 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:   16 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 309
[Close]
BLAST of smoothened-like protein vs. Planmine SMEST
Match: SMESG000040116.1 (SMESG000040116.1)

HSP 1 Score: 1304.66 bits (3375), Expect = 0.000e+0
Identity = 755/762 (99.08%), Postives = 758/762 (99.48%), Query Frame = 2 
Query:  923 QFKLESLKIGESKWCVLVFSMIXXXXXXXXXXXXXXXXXXXXVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPXXXXXXXXXXXXXENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVXXXXXXXXXXXXXGEIKGDPLSGICFISTDYMVVRXXXXXXXXXXXXXXXXXXXXXXXXXXKLIQKHLNVYATNQYKVHSNRMNKTVTFYXXXXXXXXXXXXXXXYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHXXXXXXXXXXXXXXXXXLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLF 3208
            Q +   ++IGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLF
Sbjct:  304 QIREPQVQIGESKWCVLVFSMIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSSKIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDLINVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVRICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNKTVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLMPAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFLIWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSIWNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQDGSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNSYAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRLSCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKHKKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNLETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVMIWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSAVPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLF 1065

HSP 2 Score: 523.087 bits (1346), Expect = 5.927e-167
Identity = 293/294 (99.66%), Postives = 293/294 (99.66%), Query Frame = 1 
Query:   64 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVXXXXXXXXXXDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGXXXXXXXXXXXXXXGFHQYQLSVYKWHPMXXXXXXXXXXXXXXXXGWMMQFIPNLGQSIVCRSDGAVQIREPQ 945
            CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYT QRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ
Sbjct:   16 CVSSQFVNYSIFRNNFAMNYSYSREGCAEPVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWKYLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKESNKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLPTVYTDQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNLIVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQSIVCRSDGAVQIREPQ 309

HSP 3 Score: 54.299 bits (129), Expect = 6.690e-7
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 3 
Query: 3225 RLIN-LIRKMKSFQLKIQWKVLIKKVENFAVMMK 3323
            +LIN  + KMKSFQLKIQWKVLIKKVENFAVMMK
Sbjct: 1058 KLINSCLFKMKSFQLKIQWKVLIKKVENFAVMMK 1091
The following BLAST results are available for this feature:
BLAST of smoothened-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SMO1.015e-1424.82smoothened, frizzled class receptor [Source:HGNC S... [more]
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BLAST of smoothened-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of smoothened-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smo1.676e-1529.62gene:FBgn0003444 transcript:FBtr0078129[more]
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BLAST of smoothened-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smo1.010e-1526.30smoothened, frizzled class receptor [Source:ZFIN;A... [more]
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BLAST of smoothened-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SMO3.587e-1224.00smoothened, frizzled class receptor [Source:NCBI g... [more]
SMO3.703e-1224.00smoothened, frizzled class receptor [Source:NCBI g... [more]
SMO4.130e-1224.00smoothened, frizzled class receptor [Source:NCBI g... [more]
SMO4.692e-1224.00smoothened, frizzled class receptor [Source:NCBI g... [more]
SMO5.025e-1224.00smoothened, frizzled class receptor [Source:NCBI g... [more]
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BLAST of smoothened-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Smo3.389e-1525.18smoothened, frizzled class receptor [Source:MGI Sy... [more]
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BLAST of smoothened-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P91682|SMO_DROME1.680e-1429.62Protein smoothened OS=Drosophila melanogaster OX=7... [more]
sp|P56726|SMO_MOUSE2.371e-1425.18Smoothened homolog OS=Mus musculus OX=10090 GN=Smo... [more]
sp|P97698|SMO_RAT2.517e-1425.18Smoothened homolog OS=Rattus norvegicus OX=10116 G... [more]
sp|Q99835|SMO_HUMAN4.873e-1424.82Smoothened homolog OS=Homo sapiens OX=9606 GN=SMO ... [more]
sp|O42224|SMO_CHICK7.303e-1224.11Smoothened homolog (Fragment) OS=Gallus gallus OX=... [more]
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BLAST of smoothened-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
D2ILS11.482e-16098.89Smoothened-like protein OS=Schmidtea mediterranea ... [more]
E2FMT35.698e-1728.98Smoothened OS=Platynereis dumerilii OX=6359 PE=2 S... [more]
T1ISZ08.673e-1727.90Uncharacterized protein OS=Strigamia maritima OX=1... [more]
R7U0G28.903e-1728.52Uncharacterized protein (Fragment) OS=Capitella te... [more]
A0A0P4WL152.257e-1627.39Uncharacterized protein OS=Scylla olivacea OX=8555... [more]
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BLAST of smoothened-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smo2.245e-1426.22smoothened, frizzled class receptor [Source:NCBI g... [more]
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BLAST of smoothened-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smo3.495e-1725.53smoothened, frizzled class receptor [Source:ZFIN;A... [more]
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BLAST of smoothened-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of smoothened-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO401194.157e-1525.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of smoothened-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smo5.528e-1826.39smoothened, frizzled class receptor [Source:NCBI g... [more]
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BLAST of smoothened-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000040116.11.193e-16699.14SMESG000040116.1[more]
SMESG000040116.12.834e-15499.14SMESG000040116.1[more]
SMESG000040116.13.692e-15498.89SMESG000040116.1[more]
SMESG000040116.11.089e-16698.89SMESG000040116.1[more]
SMESG000040116.15.927e-16799.08SMESG000040116.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30006045
GenBankACZ64512.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30006045 ID=SMED30006045|Name=smoothened-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3446bp
ATTTATATTAATTATCTAATGCTACATTTATTCAATATTTATATAATTAT
TTTATTATTCATTTGTGTTAGTTCACAATTTGTTAACTATTCTATTTTTC
GAAACAACTTTGCAATGAATTATTCCTATAGTAGGGAAGGGTGTGCTGAG
CCTGTCAGTGAATGCCAATTACTTAATAATGTATTAGAAAACTCAACATG
TTTTAATATTAAATTTACTTTTATGTATACATTTCCTAATACTAACTTTC
TTGACATTAATTATTGGTCTGCCGTTAAATCTATTCCAAAATGTTGGAAG
TATTTACAAATTTTACTATGTTCTCTGAAGTATCCTGAATGTCAAAAAAG
AAAATCTACATGGATGATTAATAAGCCATTCCGTAAAATTTGTGAAATAA
CAAAACAGGAATGCTCGATTTTGATTGTTAATAACAATAATAAAGAATCA
AACAAATCCGATTCCTTATCAACACCCTTTCAAAATTCATATTCCGTTGT
TGAAAAGACTCCTTCATTTTTAGATTGCAATAAATATCCACAGAATTGTC
CCGCTGACTCATTTAAGTTGAAAAATGATTTATACCAATGCCAATTACCG
ACTGTTTATACTGTCCAGCGAAGTTTATGGATTAAAGGAATTGATGGATG
CTCATTTCCTTGCAGAACCCCATTTTTTAGCAATGACGAATATAACCTAA
CCAAATCATTATTTGGATACGTTGGAATCATATTCTTTTTGCTTAATCTG
ATAGTATTAATTACTTTCGGTTTTCATCAGTATCAACTTTCCGTTTACAA
ATGGCATCCTATGCTGCTTAATTTGTTTATCATTCATATTTACTTGATCA
TATCCAGTATCGGATGGATGATGCAATTTATTCCAAATCTTGGTCAATCG
ATTGTCTGTCGTTCTGACGGAGCAGTTCAAATTAGAGAGCCTCAAAATTG
GTGAATCCAAATGGTGTGTTCTCGTATTCTCAATGATATACTTTTCGTTA
TTTGCCTCAAGTTTAACTTCACTTTCGTTTTTCTATTCTACTTTATCTGT
TACAAAACAATATTCACGTTTTTGGGCTCATACTCATGCCGTAAGTAATC
AAAAAAATCCTAAAAAATCTTTAGGAAGTTCAAAAATACCGTTTTTATCA
AAAAAACTTCGATTTTCGTTGAGAAAGCTTGAAAACACTGAAACTTTTAA
TTTTAATGATAATCAGGTATCAAATTTGAAAAAAGATAGAACTGATTTAA
AAATATCCGGGTTTGGAATAAATTGCGATTTGATTAATGTCAGAAATCAC
AGCTTGAGCAGTCAGTTATTTGTTGTTCTCATATCTGCTGCTCTTCTTAT
TATCAGTTTGACCATTGGTGAGATCAAAGGTGATCCGTTATCTGGAATTT
GCTTTATTTCCACTGATTATATGGTAGTTCGAATATGCCTGGCATTGCTT
CCTTTAACAATTATTTTGATAATATTATCAATTATTATTAATATAACAAT
TGGCCATTTGAAATTAATTCAGAAACATCTGAATGTTTATGCAACAAATC
AGTATAAGGTCCACTCTAACCGAATGAATAAGACAGTTACTTTTTACATA
TTTATAATAATTTTATTGTGGATATTTTGGAGTTTTTCTATCTACACGCA
TACATATATTTACAGCCATGAAAAATTGTGGTACAAAGCTTTAAAGAAAC
AATTATTATGCAGACTGAAATATTCCCTGATGCCTGCAATGATTGACAAT
GATAATGATATTACCAGATGCCCTTTAGAATCGAAACCCAGCATCAATGT
TTTTATTCTGCACAATTTCAGCTATTTTTCATTAAATTTTATATTTGTCC
TTTGCGCTATTTTCAATAAATCTTCGTTTCTTATTTGGAAATCACAATTT
CTAAAGTTATTCACAGTTCACAAAAAATCGGAACATGTATTTATTAAAAA
CGAGGGTGAAAATAATTGCATTAATAATGCTACTGAAAAGTTGTATTTTG
GCCTTCAAAATCGATCCTTACTAGATTCGATATGGAATCCTGGAATATTT
GTTTATTTATCATTCAAAAATGCAAAGAAAACAACAAAGGATCGCATTAA
ATCTACTCTCATTGAAGAGAAAACAGCTACTTTTGGCAGTGCTGAAAACA
GTTCAGCTAGTTTTTTCATTTATCAGCAAGACGGTTCATTACGTTCTTGT
GTTTCAATGCCTCCAGCAAATGTTCATCAAACTAATGTAAGATATGATCG
AGAAACTTGTCCTACCCTTGAAAATTTTTTGAAAGGCACTCAAAATCTTA
TTGGACAAAATACTGTTCGCAGATCGAATTCATATGCTGGCAAACCTTGT
ATAGTAACCAGCGGTCCTATGGGGAATATATCTATTCCAAATTCATGCTA
CCATAGTGGAATTGTTAATCAACAGAACTGTTTTTCTAAAGAATCCATTC
AAAATCAAAATATTGGTAAACAATCGAGATTGAGCTGTTTCAGTTGTAGC
AATAGCACTTGCTATCAAATCCATAATCGTGTATGTGCCGACAGTTCTCA
GTGTTCTGCATTAAATAGTTTTTATTCTGTGAATCACCTCACAAAACGTC
GAAATACCTTAGATAATTCATCTTCAAAACATAAAAAGTCATATAGAAGA
TTCTCAATGTCGTTCGAACAGAAAAATGAGAAAGGAGTGTCATCTAAATC
AACTCTCAACAGAAAAAAACGAATAGCTCGATATTCAGTGTCACCCAAAT
CTGTCACTAAGTCTTTCAATCATAATAACTTAGAAACTCAAAATTGCCAT
ATTGACCATTCTAGTCGGAATTCTTCGAGTTTTAGTGCGGAATCATACAA
TTCTTATAATTTAATGTCCCAACAAACTGGTGGAAGATGGAGAGATATAT
GTAGAACAAGAATGACCTTGCACGATGTTATGATATGGGCAAATAATGCG
TCACAGAATCATGTGCAGATGAACAATATGTGTCAGAATTCTTTATGCCC
CCACAAAAATATAAATCCAAATTTCATTCAAGGAATTAATGTATTGAATA
AACAATCTTTGGAAAAGGGCATTTTTTCAGCGGTCCCTCAAAAATCTACC
GGAGTCAATTCAGTTAAAATTGACGATTCTCTTCAAAAGAATCCCAATGA
ACCCATTACCAAGTTAGAAAAGAAGGATGCTCGAAAATTGATCAATTCGT
GCTTATTTAATGAATCAAATCATTCGTCTGATAAATTTAATTCGGAAGAT
GAAGTCGTTTCAGTTGAAAATTCAGTGGAAGGTATTGATAAAGAAAGTGG
AGAATTTTGCAGTGATGATGAAGTGATGTCGCTTATTAATTCAGAAAATC
ATTAACTTCACTCTGATTTACGATATGCATTTATTTTCAATTCTTCAATG
TCCTCATTTTCTATTAATATCAATATATTAGTTATTTATTTAAAAA
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protein sequence of SMED30006045-orf-1

>SMED30006045-orf-1 ID=SMED30006045-orf-1|Name=SMED30006045-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=318bp
IYINYLMLHLFNIYIIILLFICVSSQFVNYSIFRNNFAMNYSYSREGCAE
PVSECQLLNNVLENSTCFNIKFTFMYTFPNTNFLDINYWSAVKSIPKCWK
YLQILLCSLKYPECQKRKSTWMINKPFRKICEITKQECSILIVNNNNKES
NKSDSLSTPFQNSYSVVEKTPSFLDCNKYPQNCPADSFKLKNDLYQCQLP
TVYTVQRSLWIKGIDGCSFPCRTPFFSNDEYNLTKSLFGYVGIIFFLLNL
IVLITFGFHQYQLSVYKWHPMLLNLFIIHIYLIISSIGWMMQFIPNLGQS
IVCRSDGAVQIREPQNW*
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protein sequence of SMED30006045-orf-2

>SMED30006045-orf-2 ID=SMED30006045-orf-2|Name=SMED30006045-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=791bp
MIYFSLFASSLTSLSFFYSTLSVTKQYSRFWAHTHAVSNQKNPKKSLGSS
KIPFLSKKLRFSLRKLENTETFNFNDNQVSNLKKDRTDLKISGFGINCDL
INVRNHSLSSQLFVVLISAALLIISLTIGEIKGDPLSGICFISTDYMVVR
ICLALLPLTIILIILSIIINITIGHLKLIQKHLNVYATNQYKVHSNRMNK
TVTFYIFIIILLWIFWSFSIYTHTYIYSHEKLWYKALKKQLLCRLKYSLM
PAMIDNDNDITRCPLESKPSINVFILHNFSYFSLNFIFVLCAIFNKSSFL
IWKSQFLKLFTVHKKSEHVFIKNEGENNCINNATEKLYFGLQNRSLLDSI
WNPGIFVYLSFKNAKKTTKDRIKSTLIEEKTATFGSAENSSASFFIYQQD
GSLRSCVSMPPANVHQTNVRYDRETCPTLENFLKGTQNLIGQNTVRRSNS
YAGKPCIVTSGPMGNISIPNSCYHSGIVNQQNCFSKESIQNQNIGKQSRL
SCFSCSNSTCYQIHNRVCADSSQCSALNSFYSVNHLTKRRNTLDNSSSKH
KKSYRRFSMSFEQKNEKGVSSKSTLNRKKRIARYSVSPKSVTKSFNHNNL
ETQNCHIDHSSRNSSSFSAESYNSYNLMSQQTGGRWRDICRTRMTLHDVM
IWANNASQNHVQMNNMCQNSLCPHKNINPNFIQGINVLNKQSLEKGIFSA
VPQKSTGVNSVKIDDSLQKNPNEPITKLEKKDARKLINSCLFNESNHSSD
KFNSEDEVVSVENSVEGIDKESGEFCSDDEVMSLINSENH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000062esophagus
PLANA:0000099neuron
PLANA:0000103central nervous system
Vocabulary: INTERPRO
TermDefinition
IPR015526Frizzled/SFRP
IPR026544SMO
IPR000539Frizzled/Smoothened_TM
IPR020067Frizzled_dom
Vocabulary: biological process
TermDefinition
GO:0007166cell surface receptor signaling pathway
GO:0007224smoothened signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000539Frizzled/Smoothened, transmembrane domainSMARTSM01330Frizzled_2coord: 1..312
e-value: 0.0032
score: -141.2
IPR000539Frizzled/Smoothened, transmembrane domainPFAMPF01534Frizzledcoord: 116..305
e-value: 1.1E-13
score: 50.9
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 92..311
e-value: 2.3E-24
score: 87.7
NoneNo IPR availableCDDcd150307tmF_SMO_homologcoord: 121..306
e-value: 7.4054E-9
score: 56.1485
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.53
NoneNo IPR availableTMHMMTMhelixcoord: 4..23
NoneNo IPR availableTMHMMTMhelixcoord: 204..226
NoneNo IPR availableTMHMMTMhelixcoord: 146..168
NoneNo IPR availableTMHMMTMhelixcoord: 112..131
IPR015526Frizzled/secreted frizzled-related proteinPANTHERPTHR11309FRIZZLEDcoord: 116..316
IPR026544SmoothenedPANTHERPTHR11309:SF35SMOOTHENED HOMOLOGcoord: 116..316