SMED30005955

Overview
NameSMED30005955
Smed IDSMED30005955
Length (bp)1748
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005955 (SMED30005955) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005955 (SMED30005955) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005955

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30005955SMESG000029728.1 dd_Smed_v4_28658_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005955SMESG000029728.1 dd_Smed_v4_28658_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005955 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002169:30645..39619 +8644
Homology
[Close]
BLAST of SMED30005955 vs. TrEMBL
Match: A0A267DQH0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008081g2 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.370e-11
Identity = 116/504 (23.02%), Postives = 206/504 (40.87%), Query Frame = 2 
Query:  185 IVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYP------------DLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNL--FKITNSSSTLSLAFIPISSF---------------------QKLSKRAPQFF--KEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKN-----ETATNKE---ENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFL-----------GSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNP-----LEEHEHTQHFILPKDALSTLQKFRASR 1513
            ++  Q   F  L  II G   VS D  + R  YP            + +  +RK  VN +  +K+L    I   ++   +   R+    + +++++  +  GD IGDFE+ L L+ +    +      TL L    I                        Q+L +   Q     E   T  +  F  S D+  S L     T L+ +A R+S          +     KL+  + +AL+   +P S+ +     Q   YW ++++E K E+++E+Q +    R   H  ARS + L   K++     E   +KE    + + +G   + ++ +  +        S+  +LK +++ K     L+       + + ++  P  S               FD ETSD  L  +E+R+  FL            + P T++  G++ L+RY ++ + +P  GGT+LV+ K C          H+HT+ ++LP +AL  LQ   A R
Sbjct:  260 VIVRQGQAFSALHFIIRGVVSVSVDSSQHRRQYPVQFTTWKPASELEALLARRKPRVNCE--QKSLQQEAIRVRRELGYSEAERL---ATGRNRHLAFVEEGDCIGDFEYVLGLHTHSCSHRAMGEVETLILDLQNIERLITRRNQRTVNLLKLLVLEKVHQRLHQLGRQAVTPSEPASTDDVTLFAMSADRLQSELARRPPTALEMAARRQSATTRLTQRDREMATLSKLFLLDKAALIRPCVPGSVGVRLASHQQSRYWSRRLRE-KEERDREAQ-EDRVARREFHVQARSLRQLTNSKRDSEPEDELKLHKELQKTSARSQGNIVNEILETTTVQNASGEKISLMVMLKMMQRLKEVRRRLSLKSSGGERQRQSQQRPSSSG--------SAASATFDEETSDAVLVGLEDRIGDFLRRCSIHREESQSTAPATTE--GVTKLRRYDLDPEKVPLPGGTVLVKRKPCTAGTVSSEHGHQHTRRYMLPNNALRILQAHSAVR 746
[Close]
BLAST of SMED30005955 vs. TrEMBL
Match: A0A267DKR4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008081g3 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.383e-11
Identity = 115/504 (22.82%), Postives = 205/504 (40.67%), Query Frame = 2 
Query:  185 IVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYP------------DLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNL--FKITNSSSTLSLAFIPISSF---------------------QKLSKRAPQFF--KEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKN-----ETATNKE---ENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFL-----------GSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNP-----LEEHEHTQHFILPKDALSTLQKFRASR 1513
            ++  Q   F  L  II G   VS D  + R  YP            + +  +RK  VN +  +K+L    I   ++   +   R+    + +++++  +  GD IGDFE+ L L+ +    +      TL L    I                        Q+L +   Q     E   T  +  F  S D+  S L     T  + +A R+S          +     KL+  + +AL+   +P S+ +     Q   YW ++++E K E+++E+Q +    R   H  ARS + L   K++     E   +KE    + + +G   + ++ +  +        S+  +LK +++ K     L+       + + ++  P  S               FD ETSD  L  +E+R+  FL            + P T++  G++ L+RY ++ + +P  GGT+LV+ K C          H+HT+ ++LP +AL  LQ   A R
Sbjct:  260 VIVRQGQAFSALHFIIRGVVSVSVDSSQHRRQYPVQFATWKPASELEALLARRKPRVNCE--QKSLQQEAIRVRRELGYSEAERL---ATGRNRHLAFVEEGDCIGDFEYVLGLHTHSCSHRAMGEVETLILDLQNIERLITRRHQRTLNLLKLLVLEKVHQRLHQLGRQAVTPSEPASTDDVTLFAMSADRLQSELARRPPTASEMAARRQSATTRLTQRDREMATLSKLFLLDKAALIRPCVPGSVGVRLASHQQSRYWSRRLRE-KEERDREAQ-EDRVARREFHVQARSLRQLTNSKRDSEPEDELKLHKELQKTSARSQGNIVNEILETTTVQNASGEKISLMVMLKMMQRLKEVRRRLSLKSSGGERQRQSQQRPSSSG--------SAASATFDEETSDAVLVGLEDRIGDFLRRCSIHREESQSTAPATTE--GVTKLRRYDLDPEKVPLPGGTVLVKRKPCTAGTVSSEHGHQHTRRYMLPNNALRILQAHSAVR 746
[Close]
BLAST of SMED30005955 vs. TrEMBL
Match: A0A1I8HL74 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 9.969e-11
Identity = 115/504 (22.82%), Postives = 205/504 (40.67%), Query Frame = 2 
Query:  185 IVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYP------------DLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNL--FKITNSSSTLSLAFIPISSF---------------------QKLSKRAPQFF--KEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKN-----ETATNKE---ENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFL-----------GSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNP-----LEEHEHTQHFILPKDALSTLQKFRASR 1513
            ++  Q   F  L  II G   VS D  + R  YP            + +  +RK  VN +  +K+L    I   ++   +   R+    + +++++  +  GD IGDFE+ L L+ +    +      TL L    I                        Q+L +   Q     E   T  +  F  S D+  S L     T  + +A R+S          +     KL+  + +AL+   +P S+ +     Q   YW ++++E K E+++E+Q +    R   H  ARS + L   K++     E   +KE    + + +G   + ++ +  +        S+  +LK +++ K     L+       + + ++  P  S               FD ETSD  L  +E+R+  FL            + P T++  G++ L+RY ++ + +P  GGT+LV+ K C          H+HT+ ++LP +AL  LQ   A R
Sbjct:  215 VIVRQGQAFSALHFIIRGVVSVSVDSSQHRRQYPVQFATWKPASELEALLARRKPRVNCE--QKSLQQEAIRVRRELGYSEAERL---ATGRNRHLAFVEEGDCIGDFEYVLGLHTHSCSHRAMGEVETLILDLQNIERLITRRHQRTLNLLKLLVLEKVHQRLHQLGRQAVTPSEPASTDDVTLFAMSADRLQSELARRPPTASEMAARRQSATTRLTQRDREMATLSKLFLLDKAALIRPCVPGSVGVRLASHQQSRYWSRRLRE-KEERDREAQ-EDRVARREFHVQARSLRQLTNSKRDSEPEDELKLHKELQKTSARSQGNIVNEILETTTVQNASGEKISLMVMLKMMQRLKEVRRRLSLKSSGGERQRQSQQRPSSSG--------SAASATFDEETSDAVLVGLEDRIGDFLRRCSIHREESQSTAPATTE--GVTKLRRYDLDPEKVPLPGGTVLVKRKPCTAGTVSSEHGHQHTRRYMLPNNALRILQAHSAVR 701
[Close]
BLAST of SMED30005955 vs. TrEMBL
Match: A0A267DZE5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008081g1 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.318e-10
Identity = 115/504 (22.82%), Postives = 205/504 (40.67%), Query Frame = 2 
Query:  185 IVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYP------------DLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNL--FKITNSSSTLSLAFIPISSF---------------------QKLSKRAPQFF--KEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKN-----ETATNKE---ENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFL-----------GSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNP-----LEEHEHTQHFILPKDALSTLQKFRASR 1513
            ++  Q   F  L  II G   VS D  + R  YP            + +  +RK  VN +  +K+L    I   ++   +   R+    + +++++  +  GD IGDFE+ L L+ +    +      TL L    I                        Q+L +   Q     E   T  +  F  S D+  S L     T  + +A R+S          +     KL+  + +AL+   +P S+ +     Q   YW ++++E K E+++E+Q +    R   H  ARS + L   K++     E   +KE    + + +G   + ++ +  +        S+  +LK +++ K     L+       + + ++  P  S               FD ETSD  L  +E+R+  FL            + P T++  G++ L+RY ++ + +P  GGT+LV+ K C          H+HT+ ++LP +AL  LQ   A R
Sbjct:  164 VIVRQGQAFSALHFIIRGVVSVSVDSSQHRRQYPVQFATWKPASELEALLARRKPRVNCE--QKSLQQEAIRVRRELGYSEAERL---ATGRNRHLAFVEEGDCIGDFEYVLGLHTHSCSHRAMGEVETLILDLQNIERLITRRHQRTLNLLKLLVLEKVHQRLHQLGRQAVTPSEPASTDDVTLFAMSADRLQSELARRPPTASEMAARRQSATTRLTQRDREMATLSKLFLLDKAALIRPCVPGSVGVRLASHQQSRYWSRRLRE-KEERDREAQ-EDRVARREFHVQARSLRQLTNSKRDSEPEDELKLHKELQKTSARSQGNIVNEILETTTVQNASGEKISLMVMLKMMQRLKEVRRRLSLKSSGGERQRQSQQRPSSSG--------SAASATFDEETSDAVLVGLEDRIGDFLRRCSIHREESQSTAPATTE--GVTKLRRYDLDPEKVPLPGGTVLVKRKPCTAGTVSSEHGHQHTRRYMLPNNALRILQAHSAVR 650
[Close]
BLAST of SMED30005955 vs. TrEMBL
Match: A0A1I8HTG4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.487e-10
Identity = 115/504 (22.82%), Postives = 205/504 (40.67%), Query Frame = 2 
Query:  185 IVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYP------------DLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNL--FKITNSSSTLSLAFIPISSF---------------------QKLSKRAPQFF--KEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKN-----ETATNKE---ENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFL-----------GSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNP-----LEEHEHTQHFILPKDALSTLQKFRASR 1513
            ++  Q   F  L  II G   VS D  + R  YP            + +  +RK  VN +  +K+L    I   ++   +   R+    + +++++  +  GD IGDFE+ L L+ +    +      TL L    I                        Q+L +   Q     E   T  +  F  S D+  S L     T  + +A R+S          +     KL+  + +AL+   +P S+ +     Q   YW ++++E K E+++E+Q +    R   H  ARS + L   K++     E   +KE    + + +G   + ++ +  +        S+  +LK +++ K     L+       + + ++  P  S               FD ETSD  L  +E+R+  FL            + P T++  G++ L+RY ++ + +P  GGT+LV+ K C          H+HT+ ++LP +AL  LQ   A R
Sbjct:  427 VIVRQGQAFSALHFIIRGVVSVSVDSSQHRRQYPVQFATWKPASELEALLARRKPRVNCE--QKSLQQEAIRVRRELGYSEAERL---ATGRNRHLAFVEEGDCIGDFEYVLGLHTHSCSHRAMGEVETLILDLQNIERLITRRHQRTLNLLKLLVLEKVHQRLHQLGRQAVTPSEPASTDDVTLFAMSADRLQSELARRPPTASEMAARRQSATTRLTQRDREMATLSKLFLLDKAALIRPCVPGSVGVRLASHQQSRYWSRRLRE-KEERDREAQ-EDRVARREFHVQARSLRQLTNSKRDSEPEDELKLHKELQKTSARSQGNIVNEILETTTVQNASGEKISLMVMLKMMQRLKEVRRRLSLKSSGGERQRQSQQRPSSSG--------SAASATFDEETSDAVLVGLEDRIGDFLRRCSIHREESQSTAPATTE--GVTKLRRYDLDPEKVPLPGGTVLVKRKPCTAGTVSSEHGHQHTRRYMLPNNALRILQAHSAVR 913
[Close]
BLAST of SMED30005955 vs. Planmine SMEST
Match: SMESG000029728.1 (SMESG000029728.1)

HSP 1 Score: 990.334 bits (2559), Expect = 0.000e+0
Identity = 499/500 (99.80%), Postives = 500/500 (100.00%), Query Frame = 2 
Query:   35 DLSFVRQKYRNSTINPILMKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS 1534
            +LSFVRQKYRNSTINPILMKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLSDDESEDKDKEIDFDNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS
Sbjct:   12 NLSFVRQKYRNSTINPILMKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLSDDESEDKDKEIDFDNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS 511
[Close]
BLAST of SMED30005955 vs. Planmine SMEST
Match: SMESG000029728.1 (SMESG000029728.1)

HSP 1 Score: 956.051 bits (2470), Expect = 0.000e+0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 2 
Query:   89 MKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLXXXXXXXXXXXXXXXNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS 1534
            MKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLSDDESEDKDKEIDFDNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS
Sbjct:    1 MKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEGECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDINRLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSLAFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKLKCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMKQSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNETATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNILTTNKIAKSKSQIAKGLPEQSMLSDDESEDKDKEIDFDNETSDKHLNSIENRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLEEHEHTQHFILPKDALSTLQKFRASRPHTTERS 482
The following BLAST results are available for this feature:
BLAST of SMED30005955 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DQH01.370e-1123.02Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DKR47.383e-1122.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HL749.969e-1122.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DZE51.318e-1022.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HTG41.487e-1022.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30005955 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005955 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000029728.10.000e+099.80SMESG000029728.1[more]
SMESG000029728.10.000e+0100.00SMESG000029728.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005955 ID=SMED30005955|Name=SMED30005955|organism=Schmidtea mediterranea sexual|type=transcript|length=1748bp
GTAAATATCACATTTTTATTCATTTATTCTTTCAGATCTTTCATTTGTCA
GACAGAAATATCGCAATTCTACGATAAATCCAATTTTAATGAAGGATACA
CCAAATAACGGCAAAAATATTTTTTTGAATATTGACGAATTAAACTTGTC
AGATTATCAGATAATTCGCATTGAACCGGGCAAAATTGTGAGCAACCAAT
CGGATAAATTTGACGGGCTGAGAATTATCATCGAAGGGGAATGTTATGTT
TCAGGGGACCCGGTGAAAATCCGTGAAATTTACCCGGATCTTATGACGGA
TCAGAGAAAACTTGTTGTAAACATCCAGTCACTGAAGAAAAATCTCAACA
ACTTTATCATACCCTCAATAAAAGATCACGATATCAACCGGCTCATTAGA
GTTGAAGGGAAACCAAGTTATAAGAGCAAATATATTCGTACACTCGCAAG
AGGAGACAAAATAGGGGATTTTGAGTTTTGTTTGAAATTAAACAATAATT
TGTTTAAAATAACAAATTCATCTTCTACTTTATCTCTGGCATTTATCCCT
ATTTCTTCGTTTCAAAAGCTTTCAAAACGCGCGCCTCAATTTTTCAAAGA
AATCTTGGAAACCGTGACTTTGAAATGTTTTACATTCTCAAATGATAAAG
ATTTTAGCATTTTATTTGATAACATTCAAACAAAATTAAAATGTTCAGCT
CATAGAAAATCTGAGAAACGTCCCCCCAAAGCATGCATCACTGATTTCGA
AGCAACCATAAAATTGTATGAAGCAAACAGTTCCGCTCTTGTGCCATCTT
ACATTCCTAATTCAATGAGCATGATGTGGAAAATGAAACAGTCTGAAGAA
TACTGGAGAAAGAAAATTCAAGAGAGAAAATATGAACAGGAAAAAGAAAG
TCAAATAAAGATTAATTTCTTTCGATCATCTCATCATCAGAGTGCTCGAA
GCAACAAAGCTCTAGAAAAACTGAAAAAGAATGAAACGGCAACTAATAAA
GAAGAAAATCAACAAATACGAGGCTATTGCGGGTCAGGAGTTATAACGTC
AGCAAAAATCCCAATCAATCATCCATCCGTAGCAAGCGTTTGGACCGTTC
TTAAAGCCGTGAGAAAGTTCAAGCACAACTCAAATATTTTAACCACCAAC
AAAATCGCCAAATCTAAAAGTCAAATAGCGAAAGGTTTACCCGAGCAATC
TATGCTTTCGGACGACGAGTCTGAAGATAAAGACAAGGAAATTGACTTCG
ACAATGAAACTAGTGACAAACATTTGAATTCCATTGAAAATAGAATGAAA
ACATTTCTTGGTTCTTTTCCTAATACTTCGAAATCAAATGGGATCTCAAC
TTTGAAACGATATGTAGTTGAGGGAAAGTCTATTCCGCAGCTCGGAGGGA
CAATTTTGGTACGATATAAGCAATGCAACCCCCTCGAAGAACATGAGCAT
ACCCAACATTTTATTTTGCCGAAAGATGCTCTATCTACATTACAAAAGTT
CAGAGCTTCCAGACCTCACACCACAGAAAGGTCCTAAGGTTTCTATACTG
AAAGATTCATGGAGAGATCGGATAACTAAATATTTTTATTGAAAACTTTA
ATAGCTTGATGCGATGATATAAATAATGCATGAATGTTGAATCTTTTCAT
AAAATCTTTGATAACAGATAATGCCAGTCAGTTCTTTACCTGATAACTTT
CACTTTTGTCAATCTTTGCTTTGCGACTGGACGTCAATTCAAACAAGA
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protein sequence of SMED30005955-orf-1

>SMED30005955-orf-1 ID=SMED30005955-orf-1|Name=SMED30005955-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=483bp
MKDTPNNGKNIFLNIDELNLSDYQIIRIEPGKIVSNQSDKFDGLRIIIEG
ECYVSGDPVKIREIYPDLMTDQRKLVVNIQSLKKNLNNFIIPSIKDHDIN
RLIRVEGKPSYKSKYIRTLARGDKIGDFEFCLKLNNNLFKITNSSSTLSL
AFIPISSFQKLSKRAPQFFKEILETVTLKCFTFSNDKDFSILFDNIQTKL
KCSAHRKSEKRPPKACITDFEATIKLYEANSSALVPSYIPNSMSMMWKMK
QSEEYWRKKIQERKYEQEKESQIKINFFRSSHHQSARSNKALEKLKKNET
ATNKEENQQIRGYCGSGVITSAKIPINHPSVASVWTVLKAVRKFKHNSNI
LTTNKIAKSKSQIAKGLPEQSMLSDDESEDKDKEIDFDNETSDKHLNSIE
NRMKTFLGSFPNTSKSNGISTLKRYVVEGKSIPQLGGTILVRYKQCNPLE
EHEHTQHFILPKDALSTLQKFRASRPHTTERS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
Vocabulary: INTERPRO
TermDefinition
IPR014710RmlC-like_jellyroll
IPR018490cNMP-bd-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 289..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..382
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILYSSF51206cAMP-binding domain-likecoord: 21..177