Peptidase M60 domain-containing protein

Overview
NamePeptidase M60 domain-containing protein
Smed IDSMED30005938
Length (bp)300
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Peptidase M60 domain-containing protein (SMED30005938) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30005938) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005938

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
tail regionSMED30005938h1SMcG0015805 SmedASXL_005560SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
tail regionSMED30005938h1SMcG0015805 SmedASXL_017335SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30005938h1SMcG0015805 dd_Smed_v4_13583_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005938h1SMcG0015805 dd_Smed_v4_13583_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30005938h1SMcG0015805 dd_Smed_v4_15156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005938h1SMcG0015805 dd_Smed_v4_15156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30005938h1SMcG0015805 dd_Smed_v4_14792_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005938h1SMcG0015805 dd_Smed_v4_14792_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30005938h1SMcG0015805 dd_Smed_v4_13376_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005938h1SMcG0015805 dd_Smed_v4_13376_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30005938h1SMcG0015805 dd_Smed_v6_13376_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
tail regionSMED30005938h1SMcG0015805 dd_Smed_v6_13583_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
tail regionSMED30005938h1SMcG0015805 dd_Smed_v6_15156_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
secretory systemSMED30005938h1SMcG0015805 dd_Smed_v6_13583_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG4 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 2.478e-49
Identity = 88/101 (87.13%), Postives = 89/101 (88.12%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYD-MKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYG ESTNV VPTRPVVDS SL PITKD+FKIAPIIR NVPIA IP YSSAVIYLNRNCEPIIYD   SL FGVACESGKGRM
Sbjct:  141 GNPFDYDPIERLYLCDIEVYGVESTNVAVPTRPVVDSSSLDPITKDFFKIAPIIRNNVPIATIPYYSSAVIYLNRNCEPIIYDNTTSLAFGVACESGKGRM 241
[Close]
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG0 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.869e-7
Identity = 29/85 (34.12%), Postives = 47/85 (55.29%), Query Frame = 1 
Query:   28 ERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMK-SLPFGVAC 279
            E L   +IE YG+     P   +P + S  +   +++ F +A  +RKN+P+  +  YS   + +N  CEPII+D+K +L F  AC
Sbjct:  156 EELNFYEIEAYGQLIGAGPQFAKPTIPSQLVQKASRNDFPLARELRKNIPVMKVDGYSMGFVKINEKCEPIIWDVKWNLTFAFAC 240
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BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 205.297 bits (521), Expect = 4.282e-63
Identity = 98/100 (98.00%), Postives = 98/100 (98.00%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVP APIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM
Sbjct:  143 GNPFDYDPIERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPTAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 242
[Close]
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 205.297 bits (521), Expect = 5.193e-63
Identity = 98/100 (98.00%), Postives = 98/100 (98.00%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVP APIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM
Sbjct:  140 GNPFDYDPIERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPTAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 239
[Close]
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042235.1 (SMESG000042235.1)

HSP 1 Score: 202.986 bits (515), Expect = 1.913e-62
Identity = 98/100 (98.00%), Postives = 98/100 (98.00%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYGEESTNV VPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM
Sbjct:   80 GNPFDYDPIERLYLCDIEVYGEESTNVLVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 179
[Close]
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1)

HSP 1 Score: 193.741 bits (491), Expect = 7.958e-59
Identity = 94/100 (94.00%), Postives = 94/100 (94.00%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYGEESTNVPV T PVVDSVSLVPITKDYFKIAPIIRKNVPIA IPP SSAVIYLNRNCE IIYDMKSLPFGVACESGKGRM
Sbjct:  140 GNPFDYDPIERLYLCDIEVYGEESTNVPVQTIPVVDSVSLVPITKDYFKIAPIIRKNVPIAQIPPKSSAVIYLNRNCETIIYDMKSLPFGVACESGKGRM 239
[Close]
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1)

HSP 1 Score: 193.741 bits (491), Expect = 8.066e-59
Identity = 94/100 (94.00%), Postives = 94/100 (94.00%), Query Frame = 1 
Query:    1 GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKIAPIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM 300
            GNPFDYDP ERLYLCDIEVYGEESTNVPV T PVVDSVSLVPITKDYFKIAPIIRKNVPIA IPP SSAVIYLNRNCE IIYDMKSLPFGVACESGKGRM
Sbjct:  143 GNPFDYDPIERLYLCDIEVYGEESTNVPVQTIPVVDSVSLVPITKDYFKIAPIIRKNVPIAQIPPKSSAVIYLNRNCETIIYDMKSLPFGVACESGKGRM 242
The following BLAST results are available for this feature:
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A1S6KMG42.478e-4987.13Peptidase M60 domain-containing protein OS=Schmidt... [more]
A0A1S6KMG03.869e-734.12Peptidase M60 domain-containing protein OS=Schmidt... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042174.14.282e-6398.00SMESG000042174.1[more]
SMESG000042174.15.193e-6398.00SMESG000042174.1[more]
SMESG000042235.11.913e-6298.00SMESG000042235.1[more]
SMESG000042170.17.958e-5994.00SMESG000042170.1[more]
SMESG000042170.18.066e-5994.00SMESG000042170.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005938 ID=SMED30005938|Name=Peptidase M60 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=300bp
GGAAATCCGTTTGATTATGATCCGTTCGAACGTTTGTATTTGTGTGACAT
AGAAGTCTATGGAGAGGAGTCGACAAATGTTCCTGTACCAACTAGACCTG
TTGTTGATAGTGTGTCTCTAGTTCCAATAACAAAAGATTACTTCAAAATT
GCACCAATCATTCGGAAAAATGTTCCAATAGCCCCAATTCCTCCTTATAG
CTCGGCTGTTATTTACCTTAACAGAAATTGTGAACCAATAATTTACGATA
TGAAATCCCTACCATTTGGAGTTGCATGTGAAAGTGGAAAGGGAAGAATG
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protein sequence of SMED30005938-orf-1

>SMED30005938-orf-1 ID=SMED30005938-orf-1|Name=SMED30005938-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
GNPFDYDPFERLYLCDIEVYGEESTNVPVPTRPVVDSVSLVPITKDYFKI
APIIRKNVPIAPIPPYSSAVIYLNRNCEPIIYDMKSLPFGVACESGKGRM
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000421tail
PLANA:0003117parapharyngeal cell