4-coumarate--CoA ligase family protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005912 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Match: SLC27A6 (solute carrier family 27 member 6 [Source:HGNC Symbol;Acc:HGNC:11000]) HSP 1 Score: 82.0333 bits (201), Expect = 2.707e-15 Identity = 59/237 (24.89%), Postives = 109/237 (45.99%), Query Frame = 1
Query: 238 YTYSDVKGLVSSIASA-LTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-GYETV-DKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINK----AHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLV 927
YTY DV S +A L +K+G ++ +N P++V + + L+ +GC V N N S ++N + P + + L V ++ LS +++ + D +P G ++ +KL P + L L ++SGTTGLPK +++ ++ + AH+I + I LPLYH + ++ IS G+ V + KF + + ++Y ++V +
Sbjct: 80 YTYQDVDKRSSRVAHVFLNHSSLKKGDTVALLMSNEPDFVHVWFGLAKLGCVVAFLNTNIRSNSLLNCIRACGPRALVVGADLLGTVEEILPSLSENISVWGMKDSVPQGVISLKEKLSTSPDEPVPRSHHVVSLLKSTCLYIFTSGTTGLPKAAVISQLQVLRGSAVLWAFGCTAHDI-----VYITLPLYHSSAAILGISGCVELGATCVLKKKFSASQFWSDCKKYDVTVFQYI 311
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Match: SLC27A6 (solute carrier family 27 member 6 [Source:HGNC Symbol;Acc:HGNC:11000]) HSP 1 Score: 82.0333 bits (201), Expect = 2.707e-15 Identity = 59/237 (24.89%), Postives = 109/237 (45.99%), Query Frame = 1
Query: 238 YTYSDVKGLVSSIASA-LTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-GYETV-DKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINK----AHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLV 927
YTY DV S +A L +K+G ++ +N P++V + + L+ +GC V N N S ++N + P + + L V ++ LS +++ + D +P G ++ +KL P + L L ++SGTTGLPK +++ ++ + AH+I + I LPLYH + ++ IS G+ V + KF + + ++Y ++V +
Sbjct: 80 YTYQDVDKRSSRVAHVFLNHSSLKKGDTVALLMSNEPDFVHVWFGLAKLGCVVAFLNTNIRSNSLLNCIRACGPRALVVGADLLGTVEEILPSLSENISVWGMKDSVPQGVISLKEKLSTSPDEPVPRSHHVVSLLKSTCLYIFTSGTTGLPKAAVISQLQVLRGSAVLWAFGCTAHDI-----VYITLPLYHSSAAILGISGCVELGATCVLKKKFSASQFWSDCKKYDVTVFQYI 311
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Match: SLC27A6 (solute carrier family 27 member 6 [Source:HGNC Symbol;Acc:HGNC:11000]) HSP 1 Score: 82.0333 bits (201), Expect = 2.707e-15 Identity = 59/237 (24.89%), Postives = 109/237 (45.99%), Query Frame = 1
Query: 238 YTYSDVKGLVSSIASA-LTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-GYETV-DKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINK----AHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLV 927
YTY DV S +A L +K+G ++ +N P++V + + L+ +GC V N N S ++N + P + + L V ++ LS +++ + D +P G ++ +KL P + L L ++SGTTGLPK +++ ++ + AH+I + I LPLYH + ++ IS G+ V + KF + + ++Y ++V +
Sbjct: 80 YTYQDVDKRSSRVAHVFLNHSSLKKGDTVALLMSNEPDFVHVWFGLAKLGCVVAFLNTNIRSNSLLNCIRACGPRALVVGADLLGTVEEILPSLSENISVWGMKDSVPQGVISLKEKLSTSPDEPVPRSHHVVSLLKSTCLYIFTSGTTGLPKAAVISQLQVLRGSAVLWAFGCTAHDI-----VYITLPLYHSSAAILGISGCVELGATCVLKKKFSASQFWSDCKKYDVTVFQYI 311
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Match: ACSF2 (acyl-CoA synthetase family member 2 [Source:HGNC Symbol;Acc:HGNC:26101]) HSP 1 Score: 82.0333 bits (201), Expect = 2.898e-15 Identity = 85/362 (23.48%), Postives = 161/362 (44.48%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIF----HARTIITSKYENISY-----KK----ETIAEHVFTFCDKYSNNIAFI---DDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVM----------LIYALSSMGCSVTICNQNFTS-------REIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLD---LDSHIAL-MPYSSGTTGLPKGVMLTHNNLVANFRQIN---KAHEISDVK-KMLIALPLYHIYSQVIIISMGFFSGSQMVFQSK-FEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAP 1017
+SR++ + Q V F ++++ +SY KK +T+ + + T + A + +DVR T++ +K V AS L G+ +G G+ NS +V+ L Y L +GC + + F + ++I ++ ++QP + + + L +T VIS + PLPG +D+++A + Q+ + L H + + ++SGTTG PKG L+H N+V N + K HE + + +M++ PLYH V M G+ ++ S F L AI + + + L+ P + +LN + +++++++ A +P
Sbjct: 27 LSRSWQEARLQGVRFLSSREVDRMVSTPIGGLSYVQGCTKKHLNSKTVGQCLETTAQRVPEREALVVLHEDVR---LTFAQLKEEVDKAASGLLSIGLCKGDRLGMWGPNSYAWVLMQLATAQAMELEYVLKKVGCKALVFPKQFKTQQYYNVLKQICPEVENAQPGALKSQR--LPDLTTVIS----------VDAPLPGTLLLDEVVAAGSTRQHLDQLQYNQQFLSCHDPINIQFTSGTTGSPKGATLSHYNIVNNSNILGERLKLHEKTPEQLRMILPNPLYHCLGSVAGTMMCLMYGATLILASPIFNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSP 373
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Match: SLC27A2 (solute carrier family 27 member 2 [Source:HGNC Symbol;Acc:HGNC:10996]) HSP 1 Score: 81.6481 bits (200), Expect = 3.366e-15 Identity = 62/262 (23.66%), Postives = 116/262 (44.27%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTKR-GVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPLPGYETVDKLM--ADSGNMNPIGQINLDLDSHI-----ALMPYSSGTTGLPKGVMLTHNNLVAN----FRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKN 972
R + TY+ V + +A AL G++QG ++ N P YV L L +GC++ N N ++ +++ ++ + E + V ++ L +IY + + +D + D + PI + S + AL Y+SGTTGLPK M+TH + F KA ++ + I LP YH + +I I +G+ + ++KF + + +Y ++V+ + + +L N+ + N
Sbjct: 75 RDETLTYAQVDRRSNQVARALHDHLGLRQGDCVALLMGNEPAYVWLWLGLVKLGCAMACLNYNIRAKSLLHCFQCCGAKVLLVSPELQAAVEEILPSLKKDDVSIYYVSRT-SNTDGIDSFLDKVDEVSTEPIPE---SWRSEVTFSTPALYIYTSGTTGLPKAAMITHQRIWYGTGLTFVSGLKADDV-----IYITLPFYHSAALLIGIHGCIVAGATLALRTKFSASQFWDDCRKYNVTVIQYIGELLRYLCNSPQKPN 327
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Match: acs-6 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q23404]) HSP 1 Score: 125.561 bits (314), Expect = 1.330e-29 Identity = 102/349 (29.23%), Postives = 162/349 (46.42%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTFCDKYS----NNIAFIDDVRKTSYT-YSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGD-PLP---GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKML--------------IALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQA 1092
+ SK ++ T+ E +F C ++ + + FID T Y DV+ V+S+A+AL K G K G +A N PE+++ + A+ G +++ + FT E+ Q DS SI+FT+++ L+++ ++ V+ I + PL +D + PI +N+ +DS IAL+PYSSGTTG PKG LTH N+ A + KAH +DV + + LP YH Y + + +VF+ KF+ L I+ YK+ + LVPP ++FL + FN K +A A + E + PN QA
Sbjct: 2 VFVSKAASVKLSTTTVHERIFEACRIHASANKDAVCFIDAETTTKKKLYKDVEPTVNSLATALVKLGFKPGDVASQAFPNCPEFLVAMLAVMKCGGAMSNASAIFTDYELQLQFCDSNTSIVFTDEDRLARIRRATAKCPGVRKIICLRTFPLRTEFPENVLDYVELTQTPDQPI-NVNVSMDS-IALLPYSSGTTGRPKGCQLTHRNIGAML-DVAKAHLETDVAPAMFGKEKATWHKEHTVLLLPWYHAYGLNTMFETILLGMTGIVFK-KFDTIVMLNRIKFYKVKLAWLVPPMLIFLAKDPMVPIFNTAPFLKVIMSAG---ATAGKQLCEEVSKRFPNAWLCQA 343
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Match: acs-14 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q19339]) HSP 1 Score: 123.635 bits (309), Expect = 3.822e-29 Identity = 88/291 (30.24%), Postives = 153/291 (52.58%), Query Frame = 1
Query: 199 NNIAFID-DVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDP----LP-GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNN---LVANFRQINKAHEISDV---------KKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAP 1017
N IAF+ D K T+ ++ ++A L G K+ +A ++ N Y + G +V+ + FT E+ Q DS+ ++FT ++ L KV + + ++ I +I P LP G + ++++ S + + Q+ +D+ + + ++PYSSGTTG PKGVML+H N +++ + I+K+H I DV +K L+ LP YH+Y +++ G + S FEP N+L A++ YK+ L LVPP ++FL + F+L+S++ + AAP
Sbjct: 34 NKIAFVSGDNPKHFITFEQLRKDAFAVAMYLHSIGFKK-DVAAVVLPNVWHYTSFFIGCAINGGAVSGASALFTDYELQRQFVDSRAKVVFTYEDFLPKVLLAVKQSPNIQKIIVIPKPVGSSLPAGVVSWNEVV--STPVTALPQVPIDVHNDLLVLPYSSGTTGPPKGVMLSHYNFTSMISMYLAIDKSH-ILDVLDPNWDCYNEKALLFLPFYHVYG-FGLLNHCLLKGMTGIVMSHFEPNNFLTAVQNYKVRCLCLVPPIMVFLAKHPICDKFDLSSVQMIMAGAAP 319
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Match: acs-14 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q19339]) HSP 1 Score: 123.635 bits (309), Expect = 3.822e-29 Identity = 88/291 (30.24%), Postives = 153/291 (52.58%), Query Frame = 1
Query: 199 NNIAFID-DVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDP----LP-GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNN---LVANFRQINKAHEISDV---------KKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAP 1017
N IAF+ D K T+ ++ ++A L G K+ +A ++ N Y + G +V+ + FT E+ Q DS+ ++FT ++ L KV + + ++ I +I P LP G + ++++ S + + Q+ +D+ + + ++PYSSGTTG PKGVML+H N +++ + I+K+H I DV +K L+ LP YH+Y +++ G + S FEP N+L A++ YK+ L LVPP ++FL + F+L+S++ + AAP
Sbjct: 34 NKIAFVSGDNPKHFITFEQLRKDAFAVAMYLHSIGFKK-DVAAVVLPNVWHYTSFFIGCAINGGAVSGASALFTDYELQRQFVDSRAKVVFTYEDFLPKVLLAVKQSPNIQKIIVIPKPVGSSLPAGVVSWNEVV--STPVTALPQVPIDVHNDLLVLPYSSGTTGPPKGVMLSHYNFTSMISMYLAIDKSH-ILDVLDPNWDCYNEKALLFLPFYHVYG-FGLLNHCLLKGMTGIVMSHFEPNNFLTAVQNYKVRCLCLVPPIMVFLAKHPICDKFDLSSVQMIMAGAAP 319
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Match: acs-10 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:O02200]) HSP 1 Score: 120.939 bits (302), Expect = 3.460e-28 Identity = 99/347 (28.53%), Postives = 162/347 (46.69%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTFCDKYS----NNIAFIDDVRKTSYT-YSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGD-PLPGYETVDKLMADSGNMNPIGQINLDLDSH-IALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK--------------MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQA 1092
+ SK + + + E + C ++ + I FID T Y DV+ V+S+A+AL K G K G +A N PE+++ + A+ G +++ + FT E+ Q DS SI+FT+++ L++V +++ V+ I + PL + L P IN+ + IAL+PYSSGTTG PKG LTH N+ A I ++H ++V + +L+ LP YH Y ++ + +VF+ KF+ L I+ YK+ + LVPP ++FL + FN+ K +A A + E ++ PN QA
Sbjct: 2 VFVSKAVPLKLSTQPVHERILDSCRIHAAANKDAIVFIDAETTTKKKLYRDVEPTVNSLATALVKLGFKPGDVAAQAFPNCPEFLIAMLAVMKCGGAMSNASAIFTDYELQLQFKDSNTSIVFTDEDRLARVRRSVAKCPGVRKIICLRTFPLRAEFPENVLDFVELTQTPDQPINVVVSPDAIALLPYSSGTTGRPKGCQLTHKNISAML-DIAQSHLETEVAQAMFGKEKPTWNKEHVLLLLPWYHAYGLNTMLETILLGATGLVFK-KFDTIVMLNRIKFYKVKLAWLVPPMLIFLAKDPMVPIFNVAPYLKVIMSAG---ATAGKQLCEEVQKRFPNAWLCQA 343
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Match: acs-12 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q9XV68]) HSP 1 Score: 100.908 bits (250), Expect = 8.180e-22 Identity = 82/328 (25.00%), Postives = 147/328 (44.82%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIG--DPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVAN---FRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDT 1083
II SK N+ +T + +F +K+ ++A ID+ +T+ DV IA +L V++ + ++ SP + + S G ++ + + + E+ + +S+ +F++ L ++ ++ L+ I G D GY ++ L + P+ +I D D I +PYSSG G KG++ TH +VA +K HE L +PL H + I +G +V + F ++ I++YKI +HL P + ++ E +N+ S+E + A DAV E + E F E P+ T
Sbjct: 2 IIKSKLPNLDIPDKTFHDFLFHEWEKFREDVALIDNDTFAQFTFQDVMQKADFIAKSLVYLKVEKSEVVLLVMDWSPAAIYIALGASMAGAAIQVVSPKLQAWEMQFPVKESESRFVFSDPLGLHEIDKLMKTLNREYRIICTGSRDFANGYPIIEDLAFAAKQNLPLPKIEPDTD--IVYLPYSSGIHGKRKGIVTTHKVMVAKTMVMWNTSKHHEFRRGDTTLTMIPL-HKQCGLDAILCALLNGLTVVTEKNFCVHTFMTCIQRYKIRAVHLTPYLMNLMMFEAENHTYNIESLEWVVTGA---DAVTEELY-EEFTEIFPSVKT 322
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Match: CG6178 (gene:FBgn0039156 transcript:FBtr0084550) HSP 1 Score: 122.865 bits (307), Expect = 9.892e-29 Identity = 85/312 (27.24%), Postives = 155/312 (49.68%), Query Frame = 1
Query: 133 ENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETV---DKLMADSG-NMNP-IGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANF-RQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIE 1050
E + ++ +++ + + +D V Y+ S + + +A L K GVKQ + G+ S NS + + ++A ++G +V N ++ RE+ + ++ S+P IIF +K + +V V S+ VK I + ++ + +LM D P + D ++L+ SSGTTGLPKGV LT NL+A QI + +L +P +H + + +I+ G+++V+ KFE +L AIE+Y++ + +VPP ++FL + ++L+S+ AAPL E++ E
Sbjct: 18 ERQAQDSRSLGQYILDKYKSFGDRTVLVDAVNGVEYSASFMHKSIVRLAYILQKLGVKQNDVVGLSSENSVNFALAMFAGLAVGATVAPLNVTYSDREVDHAINLSKPKIIFASKITIDRVAKVASKNKFVKGIIALSGTSKKFKNIYDLKELMEDEKFKTQPDFTSPAANKDEDVSLIVCSSGTTGLPKGVQLTQMNLLATLDSQIQPTVIPMEEVTLLTVIPWFHAFGCLTLITTACV-GARLVYLPKFEEKLFLSAIEKYRVMMAFMVPPLMVFLAKHPIVDKYDLSSLMVLLCGAAPLSRETEDQIKE 328
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Match: pdgy (gene:FBgn0027601 transcript:FBtr0343521) HSP 1 Score: 112.849 bits (281), Expect = 2.339e-25 Identity = 83/335 (24.78%), Postives = 153/335 (45.67%), Query Frame = 1
Query: 121 TSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASAL-TKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINL------------DLDS-------HIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEI---SDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF 1056
TS ++ ++ + E+V+ K+ A + + YT++ ++ ++ A L TK +++ + I N PEY + G +VT N +T EI QL+ F+ + L + + LS L+G +P + + + P G I+ DL + + +P+SSGTTGLPKGVML+HNN+ +N Q+ + + + LP +HIY +++ G ++ F+P +++R++++Y+ S+L+LVPP LF++N+ + ++ S AAP+ RF+ F
Sbjct: 64 TSPFDPVTIPNVPLHEYVWRDFKKWERRTAAVCVITDRQYTFAQMRDASAAFAVRLQTKFNLQKPDVLAICLPNLPEYPIATLGAIEAGLTVTTVNPVYTPDEIARQLT-------FSGAKFLVGTVSGFATLSQASK--LVGRQIP----IAVVRTSAEEALPEGAIDFSELTSTQNVRYEDLKAPKEASADDMVFLPFSSGTTGLPKGVMLSHNNITSNCEQVQASLPLDLMGPQNTLPGVLPFFHIYGLTVVMLSKLGQGCRLATMPCFKPDDFMRSLDKYQGSILNLVPPIALFMINHPKLTQETAPHLKVVMSGAAPIGQHDVERFLNKF 385
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Match: pdgy (gene:FBgn0027601 transcript:FBtr0308838) HSP 1 Score: 112.849 bits (281), Expect = 2.339e-25 Identity = 83/335 (24.78%), Postives = 153/335 (45.67%), Query Frame = 1
Query: 121 TSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASAL-TKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINL------------DLDS-------HIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEI---SDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF 1056
TS ++ ++ + E+V+ K+ A + + YT++ ++ ++ A L TK +++ + I N PEY + G +VT N +T EI QL+ F+ + L + + LS L+G +P + + + P G I+ DL + + +P+SSGTTGLPKGVML+HNN+ +N Q+ + + + LP +HIY +++ G ++ F+P +++R++++Y+ S+L+LVPP LF++N+ + ++ S AAP+ RF+ F
Sbjct: 64 TSPFDPVTIPNVPLHEYVWRDFKKWERRTAAVCVITDRQYTFAQMRDASAAFAVRLQTKFNLQKPDVLAICLPNLPEYPIATLGAIEAGLTVTTVNPVYTPDEIARQLT-------FSGAKFLVGTVSGFATLSQASK--LVGRQIP----IAVVRTSAEEALPEGAIDFSELTSTQNVRYEDLKAPKEASADDMVFLPFSSGTTGLPKGVMLSHNNITSNCEQVQASLPLDLMGPQNTLPGVLPFFHIYGLTVVMLSKLGQGCRLATMPCFKPDDFMRSLDKYQGSILNLVPPIALFMINHPKLTQETAPHLKVVMSGAAPIGQHDVERFLNKF 385
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Match: pdgy (gene:FBgn0027601 transcript:FBtr0303294) HSP 1 Score: 112.849 bits (281), Expect = 2.339e-25 Identity = 83/335 (24.78%), Postives = 153/335 (45.67%), Query Frame = 1
Query: 121 TSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASAL-TKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINL------------DLDS-------HIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEI---SDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF 1056
TS ++ ++ + E+V+ K+ A + + YT++ ++ ++ A L TK +++ + I N PEY + G +VT N +T EI QL+ F+ + L + + LS L+G +P + + + P G I+ DL + + +P+SSGTTGLPKGVML+HNN+ +N Q+ + + + LP +HIY +++ G ++ F+P +++R++++Y+ S+L+LVPP LF++N+ + ++ S AAP+ RF+ F
Sbjct: 64 TSPFDPVTIPNVPLHEYVWRDFKKWERRTAAVCVITDRQYTFAQMRDASAAFAVRLQTKFNLQKPDVLAICLPNLPEYPIATLGAIEAGLTVTTVNPVYTPDEIARQLT-------FSGAKFLVGTVSGFATLSQASK--LVGRQIP----IAVVRTSAEEALPEGAIDFSELTSTQNVRYEDLKAPKEASADDMVFLPFSSGTTGLPKGVMLSHNNITSNCEQVQASLPLDLMGPQNTLPGVLPFFHIYGLTVVMLSKLGQGCRLATMPCFKPDDFMRSLDKYQGSILNLVPPIALFMINHPKLTQETAPHLKVVMSGAAPIGQHDVERFLNKF 385
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Match: pdgy (gene:FBgn0027601 transcript:FBtr0073972) HSP 1 Score: 112.849 bits (281), Expect = 2.339e-25 Identity = 83/335 (24.78%), Postives = 153/335 (45.67%), Query Frame = 1
Query: 121 TSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASAL-TKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINL------------DLDS-------HIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEI---SDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF 1056
TS ++ ++ + E+V+ K+ A + + YT++ ++ ++ A L TK +++ + I N PEY + G +VT N +T EI QL+ F+ + L + + LS L+G +P + + + P G I+ DL + + +P+SSGTTGLPKGVML+HNN+ +N Q+ + + + LP +HIY +++ G ++ F+P +++R++++Y+ S+L+LVPP LF++N+ + ++ S AAP+ RF+ F
Sbjct: 64 TSPFDPVTIPNVPLHEYVWRDFKKWERRTAAVCVITDRQYTFAQMRDASAAFAVRLQTKFNLQKPDVLAICLPNLPEYPIATLGAIEAGLTVTTVNPVYTPDEIARQLT-------FSGAKFLVGTVSGFATLSQASK--LVGRQIP----IAVVRTSAEEALPEGAIDFSELTSTQNVRYEDLKAPKEASADDMVFLPFSSGTTGLPKGVMLSHNNITSNCEQVQASLPLDLMGPQNTLPGVLPFFHIYGLTVVMLSKLGQGCRLATMPCFKPDDFMRSLDKYQGSILNLVPPIALFMINHPKLTQETAPHLKVVMSGAAPIGQHDVERFLNKF 385
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Match: slc27a2a (solute carrier family 27 member 2a [Source:ZFIN;Acc:ZDB-GENE-050706-104]) HSP 1 Score: 89.3521 bits (220), Expect = 1.013e-17 Identity = 77/270 (28.52%), Postives = 122/270 (45.19%), Query Frame = 1
Query: 205 IAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISEL--STVKNIYLIGDPLPGYETVD------KLMADSGNMNPIGQINLDLDSHI-----ALMPYSSGTTGLPKGVMLTHNNL-VANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKN 972
I F D+ YTY D + + +A+AL R G + + N+P YV AL+ +GC+V + N N SR +++ S + T E + V V+ L V + L G+ ET D ++ S PI L HI AL Y+SGTTGLPK ++TH + + +F Q SD+ + I LPLYH + +S G +V +SKF + + ++ ++V+ + + +L N E +N
Sbjct: 70 IVFGDE----RYTYRDADRISNRLANALRDR---SGQIVALFHGNAPMYVFTWLALAKLGCTVALLNTNIRSRSLVHCCECSGAKTLITAAELVPAVLEVLQSLRQQQVSVLMLSGEA----ETQDIINLTNQVSRASEEAPPI-----SLRQHITMKSPALYIYTSGTTGLPKAAVVTHEKVWMMSFLQRLSGVCSSDI--IYICLPLYHSAGFLAGLSGAIERGITVVLKSKFSASRFWDDCREHNVTVIQYIGEVMRYLCNTPEREN 321
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Match: CABZ01072083.2 (dual specificity phosphatase 26 [Source:NCBI gene;Acc:570815]) HSP 1 Score: 77.0258 bits (188), Expect = 7.617e-15 Identity = 38/100 (38.00%), Postives = 61/100 (61.00%), Query Frame = 3
Query: 2127 ITILSTMHNQPEMHSNEPKKKPEVIKYFYATKAGVDRLDKMVRCYVIKRSTRRWPMVIFFNMFDIA---AFIIWNIMN--LLEGINNKRRLLLFDLGKEM 2411
+ ++ST+H +S+E + KP++I + +T+ GVD DK+ Y +R T WPMVIFFNM D++ AF++W +N N+RR+ L DLG+ +
Sbjct: 401 VLVMSTLHRDDAENSDE-QSKPQMILDYNSTRGGVDNFDKLTSTYTCQRKTLHWPMVIFFNMLDVSAYNAFVLWMDLNPSWEAQKTNRRRIFLQDLGRAL 499 HSP 2 Score: 24.2534 bits (51), Expect = 7.617e-15 Identity = 12/28 (42.86%), Postives = 16/28 (57.14%), Query Frame = 1
Query: 2050 REVYSTKFLFQDDALLVSYGSKTYKLLL 2133
R + S+KF F D LVSY + K +L
Sbjct: 375 RAINSSKFAFTDTHTLVSYHPRKNKNVL 402 HSP 3 Score: 61.6178 bits (148), Expect = 1.786e-9 Identity = 34/78 (43.59%), Postives = 46/78 (58.97%), Query Frame = 1
Query: 1726 WKVIDRHEFQKWIGITILIGVYKSKNESVSNLWNMENRRPMFNKIMSGNRFLQILRVLRFDDAVARRMKRSKDKLQPI 1959
WK +D + Q +IGI ILIGVY+SK+ESV +LW+ RP+F M+ F RV+ F ++S DKL PI
Sbjct: 157 WKDLDWTDLQAFIGILILIGVYRSKHESVDSLWDGVLGRPIFRATMALYTFKSWSRVICFRKRDPEPGQKSGDKLAPI 234 HSP 4 Score: 21.557 bits (44), Expect = 1.786e-9 Identity = 9/44 (20.45%), Postives = 23/44 (52.27%), Query Frame = 2
Query: 1589 ILTGLTNFTQRNCDCLT*VFKLYFCSRLIDEIFGSTNKKGEKLF 1720
+ G T+F N + F+L+F + + + +N +G++++
Sbjct: 110 MTPGPTSFACSNAQDIKSTFELFFPDEIKEMLMQISNLEGKRVY 153
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Match: zgc:101540 (zgc:101540 [Source:ZFIN;Acc:ZDB-GENE-041212-67]) HSP 1 Score: 77.7962 bits (190), Expect = 3.896e-14 Identity = 66/273 (24.18%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 175 FTFCDKYSNNI------AFIDDVRKTSYTYSDVKGLVSSIASALTKRG-VKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYETV-DKLMADSGNMNPIG-QINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVA-NFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
+T D++ +++ AFI + +T Y+Y + IA L K + +G ++ N P ++ + L+ +GCSV + N N S+ +++ + +++ + V V+ L +IY++ + + G ++ DK+ S P + N+ ++ A + Y+SGTTGLPK ++TH L A F Q + DV + + LPLYH + G+ +V +SKF + + +Y ++V+ + + +L N
Sbjct: 49 YTILDRFQDSVRKNPKKAFIRFLDQT-YSYEQSDKESNKIARTLLKHADLHEGDTVALLLGNEPMFLWIWLGLAKIGCSVALLNHNIRSKSLLHCFTCCGANVLIAGADLQDAVEEVLPALREQGISIYILTEHVSSEGMTSLTDKIKQASDQPIPTDLRANVAFNTPAAYI-YTSGTTGLPKAAVITHRRLWAMAFFQSICGVKSDDV--IYVCLPLYHSSGFGLGFGGSVERGATIVLRSKFSSSQFWDDCRKYNVTVIQYIGETMRYLCN 317
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Match: zgc:158482 (zgc:158482 [Source:ZFIN;Acc:ZDB-GENE-070209-32]) HSP 1 Score: 77.7962 bits (190), Expect = 4.054e-14 Identity = 65/311 (20.90%), Postives = 134/311 (43.09%), Query Frame = 1
Query: 64 RNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNN---------IAFIDDVRKTSYTYSDVKGLVSSIASALTKR-GVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPS-IIFTNKECLSKVTNVISELSTVK-NIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHI-ALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNN 957
R F L + +++HAR + + ++ VF D++ I F + TY D + IA+AL + G++ G A + TN P +++ AL+ +GC+ + N + +I + + ++ + + + V V L + ++G G ++ + +S + + L ++ A+ Y+SGTTGLPK ++T L+A+ + + ++ + + LPLYH +I +GS MV + KF + + ++++V+ + + +L N
Sbjct: 20 RRFSPLLWPDLLYHARLLRAL----LRFESRRRRRPVFLPVDRFLQQAEARPHKPFIVF----EGQTLTYRDADRESNRIANALLRECGMRAGDTAALFITNQPAFILCWIALAKLGCACALLNTSIRDDSLIRSFRCCRGAKVLIASADLQAAVQQVCVPLQQDGVCVLMMGMECSGMRSLSRAAQESSDTAVPAAVRAHLTNNTPAVYIYTSGTTGLPKAAVITQTRLLASL-AVLPSIGVTHTDVLYVTLPLYHTAGFLIGFMGCVETGSSMVLRRKFSASRFWDECRAHEVTVVQYIGEILRYLCNT 321
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Match: slc27a4 (solute carrier family 27 member 4 [Source:ZFIN;Acc:ZDB-GENE-050417-248]) HSP 1 Score: 77.0258 bits (188), Expect = 6.322e-14 Identity = 52/260 (20.00%), Postives = 111/260 (42.69%), Query Frame = 1
Query: 190 KYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-----GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
KY N A I + +++ ++ + +A+ L ++G ++G + + N +YV L ++ +G + N N +++ ++ S + E + V S + +++ G+ P G E +D L+ + + P Q + + + Y+SGTTG+PK ++ H+ + + + LPLYH ++ I G G +V + KF + + +Y +++ + +LLN
Sbjct: 86 KYGNKTALIFEGTDEKWSFKELDEYSNRVANFLLQQGFREGDVVALFMENRHQYVGLWLGMAKIGVEAALINFNLRLEALVHCVNISSAKAVVFGSELTEAMCEVHSSMGKTVKLFVSGEWDPKRVPVGTEHLDPLLETTSTIQP-KQPDRSFTDRLFYI-YTSGTTGMPKAAIVVHSRYYRMAALVYYGFRMKPEDVLYDCLPLYHSAGNIVGIGQGLIHGMTVVIRKKFSASKFWDDCIKYNCTIVQYIGEICRYLLN 343
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Match: SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:NCBI gene;Acc:613067]) HSP 1 Score: 89.3521 bits (220), Expect = 1.298e-17 Identity = 70/289 (24.22%), Postives = 128/289 (44.29%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYET-VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQ 1068
++ YTYS + L + A AL K G+K G I N+P Y+ + ++ +G S+ N N S+ ++ S ++ E + V+ EL ++ + D + G E+ +DK+ A S P + +A+ Y+SGTTGLPK ++ H L+ EI +VK + LPLYH + +I + G+ +V + KF + + +Y ++++ + + +L N+ + + N+ + L V + F+ F E Q
Sbjct: 55 KEEVYTYSHMDKLSNQAARALRKFAGIKSGDCVAIFMANAPAYIWIWLGVAKLGSSIACLNNNIRSQSFLHCFRSSGAKVLLAEPELKDAIQEVMPELRKDHVRVFFLTDAVISEGTESFLDKVKAASDEPVPKSLRSYVSGKSLAMYIYTSGTTGLPKAALVNHYRLM----MACGLFEICNVKARDVVYCPLPLYHSSAMMIGVHGCISRGATLVLRPKFSASQFWDDCRKYNVTIVQYIGEVLRYLCNSDDDASHNVR-----MAIGNGLRTDVWSEFLRRFGEIQ 334
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Match: SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:NCBI gene;Acc:613067]) HSP 1 Score: 87.8113 bits (216), Expect = 4.218e-17 Identity = 63/251 (25.10%), Postives = 114/251 (45.42%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYET-VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
++ YTYS + L + A AL K G+K G I N+P Y+ + ++ +G S+ N N S+ ++ S ++ E + V+ EL ++ + D + G E+ +DK+ A S P + +A+ Y+SGTTGLPK ++ H L+ EI +VK + LPLYH + +I + G+ +V + KF + + +Y ++++ + + +L N
Sbjct: 51 KEEVYTYSHMDKLSNQAARALRKFAGIKSGDCVAIFMANAPAYIWIWLGVAKLGSSIACLNNNIRSQSFLHCFRSSGAKVLLAEPELKDAIQEVMPELRKDHVRVFFLTDAVISEGTESFLDKVKAASDEPVPKSLRSYVSGKSLAMYIYTSGTTGLPKAALVNHYRLM----MACGLFEICNVKARDVVYCPLPLYHSSAMMIGVHGCISRGATLVLRPKFSASQFWDDCRKYNVTIVQYIGEVLRYLCN 297
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Match: SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:NCBI gene;Acc:613067]) HSP 1 Score: 87.4261 bits (215), Expect = 5.385e-17 Identity = 63/251 (25.10%), Postives = 114/251 (45.42%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYET-VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
++ YTYS + L + A AL K G+K G I N+P Y+ + ++ +G S+ N N S+ ++ S ++ E + V+ EL ++ + D + G E+ +DK+ A S P + +A+ Y+SGTTGLPK ++ H L+ EI +VK + LPLYH + +I + G+ +V + KF + + +Y ++++ + + +L N
Sbjct: 78 KEEVYTYSHMDKLSNQAARALRKFAGIKSGDCVAIFMANAPAYIWIWLGVAKLGSSIACLNNNIRSQSFLHCFRSSGAKVLLAEPELKDAIQEVMPELRKDHVRVFFLTDAVISEGTESFLDKVKAASDEPVPKSLRSYVSGKSLAMYIYTSGTTGLPKAALVNHYRLM----MACGLFEICNVKARDVVYCPLPLYHSSAMMIGVHGCISRGATLVLRPKFSASQFWDDCRKYNVTIVQYIGEVLRYLCN 324
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Match: SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:NCBI gene;Acc:613067]) HSP 1 Score: 87.0409 bits (214), Expect = 6.960e-17 Identity = 63/251 (25.10%), Postives = 114/251 (45.42%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYET-VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
++ YTYS + L + A AL K G+K G I N+P Y+ + ++ +G S+ N N S+ ++ S ++ E + V+ EL ++ + D + G E+ +DK+ A S P + +A+ Y+SGTTGLPK ++ H L+ EI +VK + LPLYH + +I + G+ +V + KF + + +Y ++++ + + +L N
Sbjct: 107 KEEVYTYSHMDKLSNQAARALRKFAGIKSGDCVAIFMANAPAYIWIWLGVAKLGSSIACLNNNIRSQSFLHCFRSSGAKVLLAEPELKDAIQEVMPELRKDHVRVFFLTDAVISEGTESFLDKVKAASDEPVPKSLRSYVSGKSLAMYIYTSGTTGLPKAALVNHYRLM----MACGLFEICNVKARDVVYCPLPLYHSSAMMIGVHGCISRGATLVLRPKFSASQFWDDCRKYNVTIVQYIGEVLRYLCN 353
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Match: SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:NCBI gene;Acc:613067]) HSP 1 Score: 87.0409 bits (214), Expect = 7.026e-17 Identity = 63/251 (25.10%), Postives = 114/251 (45.42%), Query Frame = 1
Query: 226 RKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYET-VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLN 954
++ YTYS + L + A AL K G+K G I N+P Y+ + ++ +G S+ N N S+ ++ S ++ E + V+ EL ++ + D + G E+ +DK+ A S P + +A+ Y+SGTTGLPK ++ H L+ EI +VK + LPLYH + +I + G+ +V + KF + + +Y ++++ + + +L N
Sbjct: 107 KEEVYTYSHMDKLSNQAARALRKFAGIKSGDCVAIFMANAPAYIWIWLGVAKLGSSIACLNNNIRSQSFLHCFRSSGAKVLLAEPELKDAIQEVMPELRKDHVRVFFLTDAVISEGTESFLDKVKAASDEPVPKSLRSYVSGKSLAMYIYTSGTTGLPKAALVNHYRLM----MACGLFEICNVKARDVVYCPLPLYHSSAMMIGVHGCISRGATLVLRPKFSASQFWDDCRKYNVTIVQYIGEVLRYLCN 353
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Match: Slc27a6 (solute carrier family 27 (fatty acid transporter), member 6 [Source:MGI Symbol;Acc:MGI:3036230]) HSP 1 Score: 80.8777 bits (198), Expect = 4.889e-15 Identity = 68/318 (21.38%), Postives = 136/318 (42.77%), Query Frame = 1
Query: 238 YTYSDVKGLVSSIASALTKRG-VKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKL-----MADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIFSVSRKSSRHSVG 1173
YTY DV + IA AL +K+G + ++ +N P++V + + L+ +GC V N N +++ ++ +P+ + + L + ++ L ++ + D +P E +D L +A + P + L S L ++SGTTGLPK +++ ++ + +D + I LPLYH ++ I G+ V + KF + + ++Y ++V + +L + + + + L + V +F+ D F I++ G + G+I ++ SVG
Sbjct: 80 YTYEDVDKRSNRIAHALLNHSSLKRGDVVALLMSNEPDFVHVWFGLAKLGCVVAFLNSNLRFDSLLHCINTCEPTAVVVGGDLLGSIEEILPSLPKHVRVWGMKDSVP--EGIDSLQEKLSLASDEPVPPSHHVTSSLKS-TCLYIFTSGTTGLPKAAVISQLQVLKGSVGLWAFGCTAD-DIIYITLPLYHSSGSLLGIGGCVELGATCVLKKKFSASQFWNDCKKYNVTVFQYIGELCRYLCKQPQREGEKDHRVR--LAVGNGLSSDVWRQFL----------DRFGNIKMCELYGATEGNIVFMNHTGKIGSVG 381
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Match: Acsf2 (acyl-CoA synthetase family member 2 [Source:MGI Symbol;Acc:MGI:2388287]) HSP 1 Score: 77.7962 bits (190), Expect = 3.817e-14 Identity = 72/326 (22.09%), Postives = 135/326 (41.41%), Query Frame = 1
Query: 157 TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVM-----------------------LIYALSSMGCSVTICNQNFTS-------REIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMP----YSSGTTGLPKGVMLTHNNLVAN----FRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQS-KFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAP 1017
T+ E + ++ + A + ++ +K V AS L G+++G G+ NS +V+ L Y L +GC + + F + +++ +L +QP + E L +T VIS + PLPG +D ++A G + Q+ + P ++SGTTG PKG L+H+N+V N +++ + ++ ++++ PLYH V + G+ ++ S F L AI + K ++L+ P + +LN + +++ TSI A +P
Sbjct: 73 TVGECLDATAQRFPDREALVILHENIRLNFAQLKEEVDKAASGLLSIGLRKGDRLGMWGPNSYAWVLIQLATAQAGIILVSVNPAYQSSELEYVLRKVGCKGIVFPKQFKTQQYYDILKQVCPELEKAQPGAL--KSERLPDLTTVIS----------VDAPLPGTLLLDDIVAAGGKEQNLAQLRYNQRFLSCYDPINIQFTSGTTGNPKGATLSHHNIVNNSMLIGQRLKMPTKTAEELRLVLPSPLYHCLGSVGGTMVSMMHGATLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTSIRGGVIAGSP 386
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Match: Slc27a2 (solute carrier family 27 (fatty acid transporter), member 2 [Source:MGI Symbol;Acc:MGI:1347099]) HSP 1 Score: 74.7146 bits (182), Expect = 3.557e-13 Identity = 76/342 (22.22%), Postives = 143/342 (41.81%), Query Frame = 1
Query: 208 AFIDDVRKTSY-----------TYSDVKGLVSSIASALTKR-GVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELST-VKNIYLIGDP--LPGYETV-DKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFRE-HQPNF--DTFQAIQLVNQNGK--SMGSIFSVSRKSSRHSV 1170
AF++ RKT + TY+ V + +A AL + G++QG + N P YV + L +GC + N N ++ +++ ++ + + V V+ L +++ + G +T+ DK+ S P + + A+ Y+SGTTGLPK + H+ L + + I+ + +PLYH + +I + G+ + +SKF + + +Y ++V+ + + +L N + N ++K L V FI+ F + H F T I VN K ++G + RK +R+ +
Sbjct: 58 AFLEQARKTPHKPFLLFRDETLTYAQVDRRSNQVARALHDQLGLRQGDCVALFMGNEPAYVWIWLGLLKLGCPMACLNYNIRAKSLLHCFQCCGAKVLLASPDLQEAVEEVLPTLKKDAVSVFYVSRTSNTNGVDTILDKVDGVSAEPTPESWRSEVTFTTPAVYIYTSGTTGLPKAATINHHRLWYG-TGLAMSSGITAQDVIYTTMPLYHSAALMIGLHGCIVVGATLALRSKFSASQFWDDCRKYNVTVIQYIGELLRYLCNTPQKPNDRDHKVKKALGNG--LRGDVWREFIKRFGDIHVYEFYASTEGNIGFVNYPRKIGAVGRANYLQRKVARYEL 396
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Match: Slc27a4 (solute carrier family 27 (fatty acid transporter), member 4 [Source:MGI Symbol;Acc:MGI:1347347]) HSP 1 Score: 73.9442 bits (180), Expect = 5.911e-13 Identity = 63/305 (20.66%), Postives = 124/305 (40.66%), Query Frame = 1
Query: 64 RNFLKLSFQSVIFHARTIITSKYENISY--KKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-----GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNN 957
R F+K + + IF ++ K + Y +++T+ + ++ + A I + T +T+ + SS+A+ L RG+ G + + N E+V L ++ +G + N N + + L S+ + E S + + + L +++ G P E +D L+ D+ P D + Y+SGTTGLPK ++ H+ + + + LPLYH ++ I G +V + KF + + +Y +++ + +LLN
Sbjct: 43 RVFIK-TVRRDIFGGMVLLKVKTKVRRYLQERKTVPLLFASMVQRHPDKTALIFEGTDTHWTFRQLDEYSSSVANFLQARGLASGNVVALFMENRNEFVGLWLGMAKLGVEAALINTNLRRDALRHCLDTSKARALIFGSEMASAICEIHASLEPTLSLFCSGSWEPSTVPVSTEHLDPLLEDAPKHLPSHPDKGFTDKLFYI--YTSGTTGLPKAAIVVHSRYYRMASLVYYGFRMRPDDIVYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYNCTIVQYIGELCRYLLNQ 344
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Match: Slc27a1 (solute carrier family 27 (fatty acid transporter), member 1 [Source:MGI Symbol;Acc:MGI:1347098]) HSP 1 Score: 70.0922 bits (170), Expect = 8.965e-12 Identity = 57/264 (21.59%), Postives = 110/264 (41.67%), Query Frame = 1
Query: 190 KYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGD--P---LPGYETVDKLMADSGNMNPIGQI-NLDLDSHIALMPYSSGTTGLPKGVMLTHNNL--VANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNN 957
+ +A +D +T++ + +++A+ + G G + + PE+V L L+ G + N N + L S + E + V V +L + GD P LP + +D ++A++ P+ Q +D + + Y+SGTTGLPK ++ H+ +A F + + +DV + LPLYH ++ + G +V + KF + + +Y +V+ + +LL
Sbjct: 88 RQPERLALVDASSGICWTFAQLDTYSNAVANLFRQLGFAPGDVVAVFLEGRPEFVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGKSLLKFCSGDLGPESILPDTQLLDPMLAEA-PTTPLAQAPGKGMDDRLFYI-YTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRAADV--LYDCLPLYHSAGNIMGVGQCVIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQ 347
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Match: sp|P14912|4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1) HSP 1 Score: 147.132 bits (370), Expect = 9.048e-36 Identity = 109/319 (34.17%), Postives = 159/319 (49.84%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTF--CDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYL--IGDPLPGYETVDKLM-ADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQ------INKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVEN 1038
I SK +I Y + + H + F K + I+ ++TYS V+ L +AS L K G++QG ++ NSPEY S G T+ N FTS E+I QL SQ +I T + KV + +E KNI + I D KLM AD M ++ ++ D +AL PYSSGTTGLPKGVMLTH LV + Q N DV M+ LPL+HIYS ++ G +G ++ KF+ +L I++YK+++ VPP +L + + ++L+S+ S AAPL +E+
Sbjct: 12 IFRSKLPDI-YIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLIITQACYVDKVKDYAAE----KNIQIICIDDAPQDCLHFSKLMEADESEMP---EVVINSDDVVAL-PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDV--MICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELED 319
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Match: sp|P14913|4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1) HSP 1 Score: 146.747 bits (369), Expect = 1.513e-35 Identity = 109/319 (34.17%), Postives = 158/319 (49.53%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTF--CDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYL--IGDPLPGYETVDKLM-ADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQ------INKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVEN 1038
I SK +I Y + + H + F K + I+ ++TYS V+ L +AS L K G++QG ++ NSPEY S G T+ N FTS E+I QL S +I T + KV + +E KNI + I D KLM AD M ++ +D D +AL PYSSGTTGLPKGVMLTH LV + Q N DV M+ LPL+HIYS ++ G +G ++ KF+ +L I++YK+++ VPP +L + + ++L+S+ S AAPL +E+
Sbjct: 12 IFRSKLPDI-YIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLIITQACYVDKVKDYAAE----KNIQIICIDDAPQDCLHFSKLMEADESEMP---EVVIDSDDVVAL-PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDV--MICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELED 319
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1) HSP 1 Score: 142.124 bits (357), Expect = 5.186e-34 Identity = 106/320 (33.12%), Postives = 158/320 (49.38%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTF--CDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYET----VDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQ------INKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVEN 1038
I SK +I Y + + H + F K+S+ I+ +TY+DV+ + + S L+K G+KQG I+ NSPE+V S +G T+ N FTS E+I Q S +I T + KV + E + VK I + +L N P ++ + D +AL PYSSGTTGLPKGVMLTH LV + Q N DV +L LPL+HIYS ++ G +GS ++ KFE +L I++YK+++ VPP +L + + N++L+S+ S AAPL +E+
Sbjct: 15 IFRSKLPDI-YIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEMP--KVEISPDGVVAL-PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDV--LLCVLPLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLSSVRTVMSGAAPLGKELED 327
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Match: sp|Q54P77|4CL1_DICDI (Probable 4-coumarate--CoA ligase 1 OS=Dictyostelium discoideum OX=44689 GN=4cl1 PE=3 SV=1) HSP 1 Score: 141.739 bits (356), Expect = 7.101e-34 Identity = 94/336 (27.98%), Postives = 166/336 (49.40%), Query Frame = 1
Query: 106 ARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISEL-STVKNIYLIGDPLPGYETVDKLMADS--------------GNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFR 1059
+T TSKY NI ++ + + + + + +D + Y+ V + +A L K +K+G + G+I N PEYV + + MG ++ N ++T E+ + L+ P + K+ N + + +V+ + L+ + +D+L S G PI +I+ D+ A++P+SSGTTGL KGV L+H+N+V+N Q + E S KK ++ LP +HIY ++ + + G ++V KFEP +L I++YK+++ +VPP + + F+L+S+ FS AAPL + VE+ E F+
Sbjct: 7 GQTYFTSKYPNIIIPEKPVPQLILKHIRSKPDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLINNNGKDYPIVRIDPKKDT--AIIPFSSGTTGLFKGVCLSHHNIVSNTYQ-TQTIETSTYKKNDTVMGILPFFHIYGLMLFLMLMVKQGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKFDLSSLRTLFSGAAPLSSEVEDLIKERFK 339
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1) HSP 1 Score: 137.887 bits (346), Expect = 1.034e-32 Identity = 104/326 (31.90%), Postives = 161/326 (49.39%), Query Frame = 1
Query: 115 IITSKYENISYKKETIAEHVFTF--CDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQI----NKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPN 1074
I SK +I Y + H + F ++S+ I+ K YTY+DV+ +A+ L K+G++ I+ NSPE+V S +G T+ N FT E++ Q S II T ++KV + E + VK I + P + + N + I ++ + D +AL PYSSGTTGLPKGVMLTH LV + Q N I ML LPL+HIYS ++ G G+ ++ KF+ ++L I++YK+++ VPP +L + + +++L+S+ S AAPL +E+ T R PN
Sbjct: 11 IFRSKLPDI-YIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAP---EGCLHFSVLTQANEHDIPEVEIQPDDVVAL-PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSGAAPLGKELED----TVRAKFPN 326
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Match: A0A267GIP9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017208g2 PE=4 SV=1) HSP 1 Score: 217.624 bits (553), Expect = 4.084e-57 Identity = 123/379 (32.45%), Postives = 199/379 (52.51%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIFHARTIIT--SKYENISYKKE-TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIF----SVSRKSSRHSVG 1173
V R F L S +R++ + S I++ K+ + E++ + CD+Y + A + +Y D++ +S+AS L + G +G + + S N PEY +Y + S G +T N ++T+ E+ NQL D + + T K+ L+ VK I+L+ D PG L+ D G NP G +D D+ IA + YSSGTTGLPKGVML+H NLV N Q E + +K++ LP YH+Y QV +++ F G ++ ++F+P YL ++ YK++ +H+VPP +LF+ + + ++L+++ K AAAPL E+ FR+ PN D Q + + +GS+ S R+ R SVG
Sbjct: 5 VQRIFAGLRSSSTYRLSRSLASFRSPLPEINFPKDLSYPEYILSKCDRYPDRRAIVCANTGRGISYRDLRAGTASVASGLARLGFGRGDVMCLYSHNFPEYPTAMYGIMSAGGILTTANPDYTAAELANQLKDCKCKFLLTTKDKLAVAAEAAKASGCVKEIFLVDDKEPGTRCFKDLLDDDGKSNPSGSFKVDQDNDIAAILYSSGTTGLPKGVMLSHRNLVGNVEQSLTCVEYCEGEKLIGVLPFYHVYGQVPVLAFTFCYGYEIAIMTRFDPVKYLETLQNYKLTAIHIVPPILLFMAAHPMVEKYDLSALNKICCAAAPLRVETED----AFRKKFPNIDLTQGWGMTEIS--PVGSVTPLRQSQWRRQKRGSVG 377
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Match: A0A267DJH8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017208g3 PE=4 SV=1) HSP 1 Score: 217.624 bits (553), Expect = 4.724e-57 Identity = 123/379 (32.45%), Postives = 199/379 (52.51%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIFHARTIIT--SKYENISYKKE-TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIF----SVSRKSSRHSVG 1173
V R F L S +R++ + S I++ K+ + E++ + CD+Y + A + +Y D++ +S+AS L + G +G + + S N PEY +Y + S G +T N ++T+ E+ NQL D + + T K+ L+ VK I+L+ D PG L+ D G NP G +D D+ IA + YSSGTTGLPKGVML+H NLV N Q E + +K++ LP YH+Y QV +++ F G ++ ++F+P YL ++ YK++ +H+VPP +LF+ + + ++L+++ K AAAPL E+ FR+ PN D Q + + +GS+ S R+ R SVG
Sbjct: 5 VQRIFAGLRSSSTYRLSRSLASFRSPLPEINFPKDLSYPEYILSKCDRYPDRRAIVCANTGRGISYRDLRAGTASVASGLARLGFGRGDVMCLYSHNFPEYPTAMYGIMSAGGILTTANPDYTAAELANQLKDCKCKFLLTTKDKLAVAAEAAKASGCVKEIFLVDDKEPGTRCFKDLLDDDGKSNPSGSFKVDQDNDIAAILYSSGTTGLPKGVMLSHRNLVGNVEQSLTCVEYCEGEKLIGVLPFYHVYGQVPVLAFTFCYGYEIAIMTRFDPVKYLETLQNYKLTAIHIVPPILLFMAAHPMVEKYDLSALNKICCAAAPLRVETED----AFRKKFPNIDLTQGWGMTEIS--PVGSVTPLRQSQWRRQKRGSVG 377
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Match: A0A267F2Q4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017208g1 PE=4 SV=1) HSP 1 Score: 216.853 bits (551), Expect = 9.221e-57 Identity = 122/379 (32.19%), Postives = 200/379 (52.77%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIFHARTIIT--SKYENISYKKE-TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIF----SVSRKSSRHSVG 1173
V R F L S +R++ + S I++ K+ + E++ + CD+Y + A + +Y D++ +S+AS L++ G +G + + S N PE+ +Y + S G +T N ++T+ E+ NQL D + + T K+ L+ VK I+L+ D PG L+ D G NP G +D D+ IA + YSSGTTGLPKGVML+H NLV N Q E + +K++ LP YH+Y QV +++ F G ++ ++F+P YL ++ YK++ +H+VPP +LF+ + + ++L+++ K AAAPL E+ FR+ PN D Q + + +GS+ S R+ R SVG
Sbjct: 5 VQRIFAGLRSSSTYRLSRSLASFRSPLPEINFPKDLSYPEYILSKCDRYPDRRAIVCANTGRGISYRDLRAGTASVASGLSRLGFGRGDVMCLYSHNFPEFPTAMYGIMSAGGILTTANPDYTAAELANQLKDCKCKFLLTTKDKLAVAAEAAKASGCVKEIFLVDDKEPGTRCFKDLLDDDGKSNPSGSFKVDQDNDIAAILYSSGTTGLPKGVMLSHRNLVGNVEQSLTCVEYCEGEKLIGVLPFYHVYGQVPVLAFTFCYGYEIAIMTRFDPVKYLETLQNYKLTAIHIVPPILLFMAAHPMVEKYDLSALNKICCAAAPLRVETED----AFRKKFPNIDLTQGWGMTEIS--PVGSVTPLRQSRWRRQKRGSVG 377
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Match: A0A1I8HIZ5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 217.624 bits (553), Expect = 9.987e-57 Identity = 123/379 (32.45%), Postives = 199/379 (52.51%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIFHARTIIT--SKYENISYKKE-TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIF----SVSRKSSRHSVG 1173
V R F L S +R++ + S I++ K+ + E++ + CD+Y + A + +Y D++ +S+AS L + G +G + + S N PEY +Y + S G +T N ++T+ E+ NQL D + + T K+ L+ VK I+L+ D PG L+ D G NP G +D D+ IA + YSSGTTGLPKGVML+H NLV N Q E + +K++ LP YH+Y QV +++ F G ++ ++F+P YL ++ YK++ +H+VPP +LF+ + + ++L+++ K AAAPL E+ FR+ PN D Q + + +GS+ S R+ R SVG
Sbjct: 43 VQRIFAGLRSSSTYRLSRSLASFRSPLPEINFPKDLSYPEYILSKCDRYPDRRAIVCANTGRGISYRDLRAGTASVASGLARLGFGRGDVMCLYSHNFPEYPTAMYGIMSAGGILTTANPDYTAAELANQLKDCKCKFLLTTKDKLAVAAEAAKASGCVKEIFLVDDKEPGTRCFKDLLDDDGKSNPSGSFKVDQDNDIAAILYSSGTTGLPKGVMLSHRNLVGNVEQSLTCVEYCEGEKLIGVLPFYHVYGQVPVLAFTFCYGYEIAIMTRFDPVKYLETLQNYKLTAIHIVPPILLFMAAHPMVEKYDLSALNKICCAAAPLRVETED----AFRKKFPNIDLTQGWGMTEIS--PVGSVTPLRQSQWRRQKRGSVG 415
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Match: A0A1I8IBZ0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 217.238 bits (552), Expect = 4.435e-54 Identity = 123/379 (32.45%), Postives = 199/379 (52.51%), Query Frame = 1
Query: 58 VSRNFLKLSFQSVIFHARTIIT--SKYENISYKKE-TIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKSMGSIF----SVSRKSSRHSVG 1173
V R F L S +R++ + S I++ K+ + E++ + CD+Y + A + +Y D++ +S+AS L + G +G + + S N PEY +Y + S G +T N ++T+ E+ NQL D + + T K+ L+ VK I+L+ D PG L+ D G NP G +D D+ IA + YSSGTTGLPKGVML+H NLV N Q E + +K++ LP YH+Y QV +++ F G ++ ++F+P YL ++ YK++ +H+VPP +LF+ + + ++L+++ K AAAPL E+ F + F PN D Q + + +GS+ S R+ R SVG
Sbjct: 640 VQRIFAGLRSSSTYRLSRSLASFRSPLPEINFPKDLSYPEYILSKCDRYPDRRAIVCANTGRGISYRDLRAGTASVASGLARLGFGRGDVMCLYSHNFPEYPTAMYGIMSAGGILTTANPDYTAAELANQLKDCKCKFLLTTKDKLAVAAEAAKASGCVKEIFLVDDKEPGTRCFKDLLDDDGKSNPSGSFKVDQDNDIAAILYSSGTTGLPKGVMLSHRNLVGNVEQSLTCVEYCEGEKLIGVLPFYHVYGQVPVLAFTFCYGYEIAIMTRFDPVKYLETLQNYKLTAIHIVPPILLFMAAHPMVEKYDLSALNKICCAAAPLRVETEDAFRKKF----PNIDLTQGWGMTEIS--PVGSVTPLRQSQWRRQKRGSVG 1012
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Match: slc27a2a (very long-chain acyl-CoA synthetase-like [Source:NCBI gene;Acc:103021496]) HSP 1 Score: 91.6633 bits (226), Expect = 1.620e-18 Identity = 85/352 (24.15%), Postives = 157/352 (44.60%), Query Frame = 1
Query: 208 AFIDDVRKT-----------SYTYSDVKGLVSSIASALTKR-GVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQIN------------LDLDSHI-----ALMPYSSGTTGLPKGVMLTHNNL-VANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF---REHQPNFDTFQAIQLVNQNGK--SMGSIFSVSRKSS 1158
F+++VRK S++Y++ + L + +A A+ R GV++G + N P +V ALS +GC+V++ N N S+ +++ S + ++ + V +V+ L + L LMAD + + I N + L S + AL Y+SGTTGLPK +++H + +A+F Q K +S + I LPLYH ++ ++ G +V + KF + + + +Y ++V+ + + +L N + + + ++ F D E F++ F R + T I VN GK +MG + + +K+S
Sbjct: 58 CFLENVRKNPQKNFIVFEDQSFSYAEAERLSNRVARAVRGRCGVREGDTVALFMGNEPLFVWTWLALSKLGCTVSLLNTNIRSKSLLHCFSCCRAKVLIAGADLHDAVEDVLPVLKEQEVCVL-------------LMADHCDTDGIVSFNEEIKNASDEPLPISLRSKVTIRSPALYIYTSGTTGLPKAAVISHERVWLASFLQ--KMAGVSSDDIIYIYLPLYHSAGFLMGLTGAIERGITVVLRRKFSASQFWKDCRKYDVTVIQYIGEIMRYLCNTPKADSDRVHNVRLAFGNGIRADTWEE--FLQRFGDVRICECYGATEGNIGFVNYVGKIGAMGRVNLLMKKAS 392
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Match: slc27a6 (long-chain fatty acid transport protein 6 [Source:NCBI gene;Acc:103037195]) HSP 1 Score: 82.0333 bits (201), Expect = 1.382e-15 Identity = 57/253 (22.53%), Postives = 120/253 (47.43%), Query Frame = 1
Query: 232 TSYTYSDVKGLVSSIASALTKRG-VKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PG-YETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTH-NNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKN 972
+YTY ++ + +A ++G V++G ++ +N P++V + + LS +GC+V N N SR +++ + ++ + L + +++ L +++ + + + PG + +DKL S P + + D + L ++SGTTGLPK ++TH +L A + DV + LPLYH + ++ I G+ V + KF + + ++ +++ + +L N + +N
Sbjct: 79 ATYTYRQIEERSNKVAQVFEQQGTVRKGDTVAMLMSNEPDFVSVWFGLSKLGCAVAFLNTNIKSRSLLHCFNACGAKLLVVGADLLDTLQDILPNLHEDNISVWAMKNEISHPGVHSLLDKLENASPEPIPAERRSTDSLKNATLYIFTSGTTGLPKAAVITHLQSLKAAGGFWAYGGKSEDV--IYTPLPLYHSAASLVGIGGTIELGATCVLKKKFSASQFWNDCRKFDVTIFQYIGELCRYLCNQPKGEN 329
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Match: ENSAMXT00000000646.2 (very long-chain acyl-CoA synthetase [Source:NCBI gene;Acc:103027907]) HSP 1 Score: 81.2629 bits (199), Expect = 2.789e-15 Identity = 64/256 (25.00%), Postives = 120/256 (46.88%), Query Frame = 1
Query: 235 SYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPL--PGYETVDKLMADSGNMNPIGQINLDLDSHI-----ALMPYSSGTTGLPKGVMLTHNNLVA-NFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKN 972
+Y+YSDV + +A+AL G+K+G + N P + + L+ +GC+ + N N SR +++ S S++ ++ V ++ L ++YL+ D G +++ + + + + P ++L L S++ A Y+SGTTGLPK ++T + A +F Q DV I LPLYH +I + G+ V + KF + + +Y ++V+ + + +L N + N
Sbjct: 77 TYSYSDVDRRSNKVANALRMVAGLKEGDTVAMFLGNEPCFAWVWLGLAKLGCTAALLNFNIRSRSLLHCFSCCGASVLIAAEDLRGAVEEILPALKEKGISVYLLSDECSTDGIQSISQAIQLASD-EP---LSLSLRSNLKARSTAFYIYTSGTTGLPKAAVVTQERVWAGSFVQGVSGVTAEDV--FYINLPLYHSAGFMIGLIGCIERGNTAVLRRKFSASQFWDDCRKYDVTVMQYIGETMRYLCNTPKKDN 326
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Match: acsf2 (acyl-CoA synthetase family member 2 [Source:NCBI gene;Acc:103037666]) HSP 1 Score: 75.8702 bits (185), Expect = 1.046e-13 Identity = 74/333 (22.22%), Postives = 142/333 (42.64%), Query Frame = 1
Query: 157 TIAEHVFTFCDKYSNNIAFI---DDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMG-----------------------CSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQIN-----LDLDSHIALMPYSSGTTGLPKGVMLTHNNLVAN--FRQINKAHEISDVKKMLIALPLYHIYSQV-IIISMGFFSGSQMVF-QSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIE 1050
T+ + + ++Y + A + +RKT ++ + V A+ L G+K+G G+ N+ E+V++ YA + G C +C F +++ + L P + + + S L + + L PG + LM ++G+ + Q+ L D I + ++SGTTG PKG L+H+N+V N F + ++ ++ + +PLYH + V I MG G MVF S ++ L A++ K + ++ P + +L + F+++S++ A +P V + I+
Sbjct: 66 TVGKALRLTTERYPDREAVVFVQSGIRKT---FAQFQNDVDQAAAGLVALGLKKGDRLGMWGPNTYEWVVIQYATAKAGIIMVSVNPAYQLQELEYALRKVQCKAIVCPTQFKTQKYCDMLRQLCPGMDTASPGGIKS-----SRLPDLSTVILTDSKQPGTFSWTDLM-EAGSSDHFNQLQDIQKTLSFDDPINIQ-FTSGTTGSPKGAALSHHNIVNNAYFIGLRMGYQWRKNVRVCVPVPLYHCFGSVGGGIVMGLH-GITMVFPSSGYDGRANLEAMQNEKCTFVYGTPTMYIDMLGQPDLAKFDISSVQTGIVAGSPCPPEVMRKVIK 387
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Match: acsf2 (acyl-CoA synthetase family member 2 [Source:NCBI gene;Acc:103037666]) HSP 1 Score: 75.8702 bits (185), Expect = 1.046e-13 Identity = 74/333 (22.22%), Postives = 142/333 (42.64%), Query Frame = 1
Query: 157 TIAEHVFTFCDKYSNNIAFI---DDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMG-----------------------CSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQIN-----LDLDSHIALMPYSSGTTGLPKGVMLTHNNLVAN--FRQINKAHEISDVKKMLIALPLYHIYSQV-IIISMGFFSGSQMVF-QSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIE 1050
T+ + + ++Y + A + +RKT ++ + V A+ L G+K+G G+ N+ E+V++ YA + G C +C F +++ + L P + + + S L + + L PG + LM ++G+ + Q+ L D I + ++SGTTG PKG L+H+N+V N F + ++ ++ + +PLYH + V I MG G MVF S ++ L A++ K + ++ P + +L + F+++S++ A +P V + I+
Sbjct: 66 TVGKALRLTTERYPDREAVVFVQSGIRKT---FAQFQNDVDQAAAGLVALGLKKGDRLGMWGPNTYEWVVIQYATAKAGIIMVSVNPAYQLQELEYALRKVQCKAIVCPTQFKTQKYCDMLRQLCPGMDTASPGGIKS-----SRLPDLSTVILTDSKQPGTFSWTDLM-EAGSSDHFNQLQDIQKTLSFDDPINIQ-FTSGTTGSPKGAALSHHNIVNNAYFIGLRMGYQWRKNVRVCVPVPLYHCFGSVGGGIVMGLH-GITMVFPSSGYDGRANLEAMQNEKCTFVYGTPTMYIDMLGQPDLAKFDISSVQTGIVAGSPCPPEVMRKVIK 387
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Sea Lamprey
Match: SLC27A5 (solute carrier family 27 member 5 [Source:HGNC Symbol;Acc:HGNC:10999]) HSP 1 Score: 78.1814 bits (191), Expect = 5.225e-15 Identity = 73/292 (25.00%), Postives = 134/292 (45.89%), Query Frame = 1
Query: 205 IAFIDDVRKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNK-ECLSKVTNVISELSTVKNIYL-IGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIA-----LMPYSSGTTGLPKGVMLTHNN-LVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFS-GSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIE 1050
+ F DD +TY + V+ A AL + GV+ G ++ N P ++ +++A +S+ C T+ N N R +++ L+ S+ + T L V + E++ ++ L +G+ PG + +A + P + +HI + Y+SGTTGLPK V ++H NF DV + I LPLYH S +I +G G+++V + KF +++ ++ ++V V +LL+ + + + S+ + + A V RF+E
Sbjct: 76 LTFEDD----RWTYEEFVRDVARTAHALQEFGGVRPGDTVALLMENEPAFLFVLFACTSLACPATLMNTNLRGRMLLHCLTTSKAQFLVTGSGPLLVAVEEISVEMNELQIRPLTMGEATPG--SAVPSLASTFREAPCNLPAASVRAHITAKDAFIYIYTSGTTGLPKAVKVSHTRAWCGNFTYSMSGVRAEDV--VYIFLPLYH-SSAIIAAFIGSLDIGARLVLRKKFSASHFWGDCRRHGVTVFQYVGEICRYLLSQPKNELDRVHSVR--MAVGNGMRADVWARFLE 356
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005192.1 (pep scaffold:Pmarinus_7.0:GL479731:2711:5574:-1 gene:ENSPMAG00000004718.1 transcript:ENSPMAT00000005192.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.781 bits (164), Expect = 1.881e-12 Identity = 57/206 (27.67%), Postives = 94/206 (45.63%), Query Frame = 1
Query: 205 IAFIDDVRKTSYTYSDVKGLVSSIASALTK-RGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNK-ECLSKVTNVISELSTVKNIYL-IGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIA-----LMPYSSGTTGLPKGVMLTHNN-LVANFRQINKAHEISDVKKMLIALPLYH 795
+ F DD +TY + V+ A AL + GV+ G ++ N P ++ +++A +S+ C T+ N N R +++ L+ S+ + T L V + E++ ++ L +GD PG +A + P + +HI + Y+SGTTGLPK V ++H NF DV + I LPLYH
Sbjct: 75 LTFEDD----RWTYEEFVRDVARTAHALQEFGGVRPGDTVALLMENEPAFLFVLFACTSLACPATLMNTNLRGRMLLHCLTTSKAQFLVTGSGPLLVAVEEISVEMNELQIRPLTMGDATPGSPVPS--LASTFREAPCNLPAASVRAHITAKDAFIYIYTSGTTGLPKAVKVSHTRAWCGNFNYSMSGVRAEDV--VYIFLPLYH 272
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Sea Lamprey
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:ZFIN;Acc:ZDB-GENE-030131-7099]) HSP 1 Score: 55.0694 bits (131), Expect = 6.526e-8 Identity = 68/246 (27.64%), Postives = 113/246 (45.93%), Query Frame = 1
Query: 241 TYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEY-VMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPS--IIFTNKECLSKVTNVISELSTVKNIYLIGDPL----PGYETVDKLMADSGNMNPIGQINLDLDSHIA----LMPYSSGTTGLPKGVMLTHNNL------VANFRQINKAHEISDVKKMLIA-LPLYHIYSQV--IIISMGF-----------FSGSQMVFQSKFEPTNYL 885
TY + A + K G+++ GI+ NS E+ + I A+ + G +V I N S E ++D+ + I+ N + L K+ V S+L +K I L P + + M +G P Q++ + S A + Y+SGTTG PKGVML+H+NL Q+ A + + ++++I+ LPL HI +Q+ I + M F GS +V + PT ++
Sbjct: 112 TYKEYYAQCRQAAKSFLKLGLQRYHGVGILGFNSAEWFISDIGAILAGGFAVGIYTTN--SAEACQYVADNCLANIIVVENDKQLQKILEVQSQLPHLKAIVQYKGELKEKKPNVYSWAEFMR-AGEEVPDAQLDAIIASQRANQCCSLIYTSGTTGSPKGVMLSHDNLTWTATVTGELTQLRSADD--NYQEVVISYLPLSHIAAQLVDIWLPMRFGGVTYFAQPDALKGSLVVTMKEVRPTGFM 352
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Sea Lamprey
Match: ACSL1 (acyl-CoA synthetase long chain family member 1 [Source:HGNC Symbol;Acc:HGNC:3569]) HSP 1 Score: 54.299 bits (129), Expect = 1.435e-7 Identity = 57/230 (24.78%), Postives = 101/230 (43.91%), Query Frame = 1
Query: 241 TYSDVKGLVSSIASALTKRGVKQGT--MAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELST-----VKNIYLIGDPLPGYETVDKLMADSG-------NMNPIGQIN-----LDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFR---QINKAHEISDVKKMLIA-LPLYHIYSQVIIISM-------GFFSG 840
+Y + + S G K + + GI S N PE+++ A + V I ++ ++ S++ +K L KV ++ + VK I ++ DP G +++ G + IG+ N IA++ Y+SGTTG PKG MLTH N++++F I K + + +LI+ LPL H++ +++ S+ GF+ G
Sbjct: 124 SYKETSDRAEYLGSGFLHLGAKPSSEQVIGIFSQNRPEWIIAEQACYTYSLVVVPLYDTLGRESIDYIINQAEISMVVCDK--LEKVKGLLESIEEGAIRIVKTIVVM-DPFDG--VMEQRARKCGIDLILFRELEVIGKTNHREPIPPQPDDIAIICYTSGTTGNPKGAMLTHKNMISDFSAFLAITKDTFLPNTDDVLISFLPLAHMFERLVEASILCNGGRVGFYQG 348
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Yeast
Match: PCS60 (Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase [Source:SGD;Acc:S000000426]) HSP 1 Score: 60.4622 bits (145), Expect = 9.377e-10 Identity = 37/141 (26.24%), Postives = 68/141 (48.23%), Query Frame = 1
Query: 598 NMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNF-NLTSIEKCFSAAAP 1017
N NP+ S +AL+ ++SGTT PK V L H N+V + I ++++ + + + +PL+H++ + ++ F + +V F P + +Y + VP + +LN + F ++ I C SA AP
Sbjct: 176 NTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAP 316
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Match: EDO46392 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPW4]) HSP 1 Score: 172.94 bits (437), Expect = 9.263e-46 Identity = 99/313 (31.63%), Postives = 176/313 (56.23%), Query Frame = 1
Query: 190 KYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETV-DKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVA------NFRQI--NKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQL 1101
+Y + A ID S+T+S++ L+ S L +RG + G +I N EY ++ Y S+G VT N +T RE++ QL DSQ + I T E + +V ++ S V+ ++++ D PG++T+ D+++ D G+ P + ++ +A + YSSGTTGLPKGV+LTH NL++ NF + + E S + ++LI +P++H++ I++ + G MV +F+P ++L AI++YK++ + +VPP ++FL + ++L+S++ AAPL + + F+ F PN ++ Q L
Sbjct: 30 EYGDEKALIDSATGKSFTFSELCTLIRKCGSVLVRRGAQIGDTMAVILPNMIEYPVVCYGALSVGMRVTTLNPQYTVREMVPQLKDSQANYIITTPELIHQVNQAAAKCSCVRRVFVLAD-TPGHQTLYDQILNDDGSAFP-SHVPVNWKQDVAYILYSSGTTGLPKGVLLTHYNLISAVVILNNFWAMTSEQTTEASKIIQVLI-VPMFHVFGLAIMLGINIAIGVTMVCIRQFDPVSFLEAIQKYKVTNISVVPPLLIFLAKHPSVLKYDLSSVKSVGCGAAPLGEEMMDAFMSRF----PNVESNQGYGL 335
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Match: EDO46794 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RP51]) HSP 1 Score: 169.859 bits (429), Expect = 8.857e-45 Identity = 95/288 (32.99%), Postives = 157/288 (54.51%), Query Frame = 1
Query: 166 EHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIAL-PLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDA 1026
+V + Y A +D SYTY+ + ++ SAL ++G++ M ++ N PEY ++ Y ++S+G VT N +T+ EII QL DS + T + K ++ + VK IY G D L D G P+ ++++ + +PYSSGTTGLPKGVMLTH NL+ + + +S + + L P++H Y I++ + G+ ++ ++FEPT++L AI+++KI++L +VPP +LFL + + +NL SI++ S AAPL A
Sbjct: 22 RYVLKHMESYGEETAMVDSTSGRSYTYNKLTEMIKKCGSALIRQGLQTKDMVAVLLPNMPEYPIVFYGVTSVGGIVTTINPAYTTDEIIYQLKDSGAKYLVTVPQLAQKAKQA-ADQAGVKRIYTFGYVDGCKSLFDLLHEDDGGSYPV-SVSVNWKEDVVCLPYSSGTTGLPKGVMLTHYNLIHHAAMFSNDDVMSSEDLITLGLLPMFHSYGLSILMGVCLIKGASVICMTQFEPTHFLEAIQKFKITMLPVVPPIVLFLAKHPLVEKYNLMSIKQVTSGAAPLGA 307
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Match: EDO38127 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDT0]) HSP 1 Score: 135.576 bits (340), Expect = 2.186e-33 Identity = 96/328 (29.27%), Postives = 161/328 (49.09%), Query Frame = 1
Query: 115 IITSKYENISYKK-ETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVD---KLMADSGNMNPIGQINLDLDSHIAL-MPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKM--LIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNF 1077
I+ S Y I K ++ + + C + + A +D +YTY+D+ L+ SAL + GVK +A + N +Y + +Y ++G VT N +T E+ QL DS I T+ SK+ + E + N+ + +E+V+ L+ D G+ + + D A+ MPYSSGTTG+ KGV+ +H N + + + + K++ L LPL+H + + + M F+ GS+++ FEP L+ IE+YK++ +VPP LFL + ++L+S+E SAAAP V + T E P+
Sbjct: 4 ILRSSYPAIDVPKNQSYVQFILDCCKRNGDKEALVDGPTGETYTYTDLITLIKKCGSALLQAGVKPKDVALLHLPNIMQYPVYLYGAQAIGAVVTTANPGYTVDELAYQLIDSSAKYIITD----SKLYHTAIEAARKANVEHV------FESVEFFKDLLKDDGSK--LKGVYSPTDPTEAICMPYSSGTTGVSKGVLQSHYNYIGHALSLGSKEFMQWDKRLVTLSLLPLFHAFGLAVNVGMHFYLGSKVILLQGFEPEQLLKTIEKYKVNDFPMVPPLALFLAKHPLVDKYDLSSLESMISAAAP----VGKGILRTMAERIPSL 315
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Match: EDO33258 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSP2]) HSP 1 Score: 134.035 bits (336), Expect = 3.053e-33 Identity = 82/242 (33.88%), Postives = 133/242 (54.96%), Query Frame = 1
Query: 274 IASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINK---AHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSI 990
+ASALT++G KQG + I+ N PE+ + +A +G VT N +T RE+ +QL SQ S + T C+ + E + V N+Y++G+ G ++ +L+ D G P +++ IA +PYSSGTTGLPKGV LTH NL+A+ + H D +L LP YH Y ++ ++ G ++V +F+ +L+ I+ KI+ LVPP ++FL + ++L+S+
Sbjct: 1 MASALTRKGFKQGEVLAIMCPNIPEFAIAYFAAILIGGIVTSMNPLYTGREVAHQLVHSQASWLLTVPPCIPRAMEGAKE-AGVANVYVVGE-AEGCASLSELLEDDGTCLPT-DLSIKPREDIAALPYSSGTTGLPKGVQLTHYNLIADSCIVMGESFTHYSRD-SHVLALLPFYHSYGLMVNLANVLLVGGRVVCIQRFDQEAFLKTIQNEKITHAALVPPIMIFLAKHEMVDQYDLSSL 238
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Match: EDO30960 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZA8]) HSP 1 Score: 134.035 bits (336), Expect = 1.208e-32 Identity = 97/343 (28.28%), Postives = 163/343 (47.52%), Query Frame = 1
Query: 61 SRNFLKLSFQSVIFHARTIITSKYENISYKK-ETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDS-HIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKM--LIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNF 1077
S + +S I+ S Y I K ++ + + C + A +D ++TY+D+ L SA+ + GV + + + +Y + +Y +MG V+ N +++ E+ Q++D I TN SK+ + E + N+ + E ++ D G+ + I D+ A +PYSSGTTGL KGVM +H NL+AN + + + K++ L +PL+H + VI I M F+ GS++V FEP L+ IE+YK++ +VPP LFL + ++L+S+E SAAAP+ V + T E P+
Sbjct: 28 STKLTRPCIRSYTISGDRILRSNYPAIDVPKNQSYVQFILDSCKRNGEKDALVDGPTGETFTYTDLITLTKKCGSAMLRAGVTPKDVVLLHLPSIMQYAVYLYGAQAMGGVVSTANPGYSADELAYQVTDCDAKYIITN----SKLYHTAIEAARKANVEHV---FVSEEFFKDVLEDDGSA--LTGIYCPSDTTETACLPYSSGTTGLSKGVMQSHFNLIANALCLGSKNFMQHDKQLVTLSLMPLFHAFGLVINIGMHFYLGSKVVLLQGFEPEQLLKTIEKYKVTDFPMVPPLALFLAKHPLVDKYDLSSLESMVSAAAPVGKGV----LRTMAERIPSL 357
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Match: slc27a2a (very long-chain acyl-CoA synthetase [Source:NCBI gene;Acc:101155585]) HSP 1 Score: 86.6557 bits (213), Expect = 5.859e-17 Identity = 77/320 (24.06%), Postives = 139/320 (43.44%), Query Frame = 1
Query: 139 ISYKKETIAEHVFTFCDKYSNNIAFIDDVR-----KTSYTYSDVKGLVSSIASALTKRG-VKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPLP--GYETVDKLMADSGN--MNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKK---MLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETF 1056
+ Y+ I + D + + +A D + SYTY + +A AL+ +K G + N P V + AL+ +GC + N N SR +++ S I+ T+ + V V+ L + ++GD L G+E++ + ++ + ++P + N+ S AL Y+SGTTGLPK ++TH + + A E+ V+ + LPLYH ++ + G G V + KF +++ +Y I+V+ + + +L N + N ++ F D E F+E F
Sbjct: 37 VGYRLGQIKRRFYGILDCFLDRVAKHPDKKLFIFEDVSYTYKQADAESNKVARALSAHAQLKPGDTVALFLGNEPLLVWVWLALAKLGCVTALLNYNIRSRSLLHCFSCCGAKILITSPDLREAVEEVLPTLREQGIRVLVLGDHLEADGFESLFHKVQEASDQPLSPELRANIHHKSP-ALYIYTSGTTGLPKAAIITHQRAWS----ASLAQEMVGVRSDDIFYLYLPLYHTAGFLMGLCGGINKGVTFVLKRKFSVSSFWDDCRKYNITVIQYIGEIMRYLCNMPKKDNDKDHNVRLAFGNGIRTDTWAE--FLERF 349
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Match: ENSORLT00000006531.2 (very long-chain acyl-CoA synthetase [Source:NCBI gene;Acc:101172074]) HSP 1 Score: 82.8037 bits (203), Expect = 1.021e-15 Identity = 69/279 (24.73%), Postives = 123/279 (44.09%), Query Frame = 1
Query: 193 YSNNIAFIDDVRK-----------TSYTYSDVKGLVSSIASALTKRG-VKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVK-NIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHI-----ALMPYSSGTTGLPKGVMLTHNNL-VANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKN 972
YS F+D V++ Y+Y DV + + AL +K+G + N P +V L+ +GC + N N S+ +++ S ++ T E V ++ L ++YL+ D G + ++ L +D + + DL ++I AL Y+SGTTGLPK ++TH + A+F Q DV I LPLYH +I + G+ ++ + KF + + +Y ++V+ + + +L N + +N
Sbjct: 118 YSVLDCFLDAVKRHPTKTFLHFEGREYSYEDVDRQSNKVGRALQAEARLKEGDTVALFLPNEPCFVWTWLGLAKLGCPAALLNFNIRSKSLLHCFSCCGAKVLITCTEQQDAVEEILPTLREQGISVYLLSDK-GGVQGINPL-SDKISKASDEPLTRDLRANIHIRSTALYIYTSGTTGLPKAAVVTHERVWAASFLQAASGVTSEDV--FYINLPLYHSAGFLIGLCGSIERGNTIILRRKFSASQFWDDCRKYNVTVMQYIGETLRYLCNMPKKEN 392
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Match: ENSORLT00000037833.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:101160710]) HSP 1 Score: 78.5666 bits (192), Expect = 1.847e-14 Identity = 38/79 (48.10%), Postives = 50/79 (63.29%), Query Frame = 1
Query: 1726 WKVIDRHEFQKWIGITILIGVYKSKNESVSNLWNMENRRPMFNKIMSGNRFLQILRVLRFDDAVARRMKRSKDKLQPIK 1962
WK +D+ + +IGI IL GVY+SK E+ S+LWN EN RP+F MS F I RV+RFD+ R +R DKL I+
Sbjct: 153 WKSLDQTDLNAYIGILILAGVYRSKGEATSSLWNEENGRPIFRATMSLETFHMISRVIRFDNRDTRVGRRESDKLAAIR 231 HSP 2 Score: 75.485 bits (184), Expect = 1.633e-13 Identity = 52/158 (32.91%), Postives = 84/158 (53.16%), Query Frame = 3
Query: 1992 LSEKVTKKKLTKEIMVYRNKGSIFYKVF--ISR*CSSRLLWF-ENI*IIT----------ILSTMHNQPEMHSNEPKKKPEVIKYFYATKAGVDRLDKMVRCYVIKRSTRRWPMVIFFNMFDIA---AFIIWNIMNLL--EGINNKRRLLLFDLGKEM 2411
L E++ K+KLT V RNK + ++ R S + F E +++ ++STMH + + + KP++I + +TK GVD LDK+ Y +R T RWPM IFFN+ D++ A+++W+ +N G +RRL L +LGK +
Sbjct: 339 LGEELQKRKLTMLGTVRRNKPELPSEILKIQGRPMHSSIFAFTEKATVVSYCPKRNKNVLVMSTMHTDASLSTRD-DMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTARWPMAIFFNIVDVSAYNAYVLWSEINQEWNAGKLYRRRLFLEELGKAL 495
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Match: ENSORLT00000029842.1 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101157644]) HSP 1 Score: 77.411 bits (189), Expect = 5.805e-14 Identity = 47/144 (32.64%), Postives = 82/144 (56.94%), Query Frame = 3
Query: 2001 KVTKKKLTKEIMVYRNKGSIFYKVFISR*CSSRLLWF--ENI*IITILSTMHNQPEMHSNEPKKKPEVIKYFYATKAGVDRLDKMVRCYVIKRSTRRWPMVIFFNMFDIA---AFIIWNIM--NLLEGINNKRRLLLFDLGKEM 2411
K K +L E+ R + ++ + + C++ L + +N ++ +LST H++ E+ KKPE+I + TK GVD LDK++ Y +R T+RWP+ +F NM D++ A++IW M + + N+RR+ L LGK++
Sbjct: 606 KSNKPELPPELASARGREALSSRFAFTD-CAAALSYVPKKNKNVL-LLSTKHSRVEICHERRDKKPEMILDYNKTKGGVDNLDKLLATYSCRRMTKRWPLAMFHNMIDMSAYNAYVIWREMHPDWMPWNRNRRRIFLEQLGKDL 747 HSP 2 Score: 63.1586 bits (152), Expect = 1.224e-9 Identity = 29/78 (37.18%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 1729 KVIDRHEFQKWIGITILIGVYKSKNESVSNLWNMENRRPMFNKIMSGNRFLQILRVLRFDDAVARRMKRSKDKLQPIK 1962
K +D E + ++G+ IL GVY+S+ E++ +LW E RP+F MS F Q LRFDD R + + D+L ++
Sbjct: 405 KPMDEIELRAYVGLLILAGVYRSRGEALVSLWEPECGRPIFGATMSLRSFYQRSATLRFDDRSTRASRSASDRLAAVR 482
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Match: slc27a4 (solute carrier family 27 member 4 [Source:NCBI gene;Acc:101158619]) HSP 1 Score: 71.2478 bits (173), Expect = 3.485e-12 Identity = 50/261 (19.16%), Postives = 106/261 (40.61%), Query Frame = 1
Query: 190 KYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLP-----GYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNN 957
++ + A I + +T+ + + +A+ L +RG ++G + + N +YV L ++ +G + N N +++ + S + E VT V + ++ GD P G E ++ L+ + + P D + Y+SGTTG+PK ++ H+ + ++ + LPLYH ++ + G +V + KF + + +Y +++ + +LLN
Sbjct: 88 RHGDKTALIFEGTGERWTFRQLDEYSNRVANLLLERGFREGDVVALFMENRAQYVGLWLGMAKVGIEAALINFNLRLEALVHCIIISNAKAVVFGSELTDAVTEVHKSMGKAVQMFCCGDWDPKRVPQGTECLEPLLDGAPSHLPRRPQRCFTDRLFYI--YTSGTTGMPKAAIVVHSRYYRMAALVYYGFRMTSEDVVYDCLPLYHSAGNIVGVGQCVIHGMTVVIRKKFSASRFWDDCVKYNCTIVQYIGEICRYLLNQ 346
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Match: SMESG000041777.1 (SMESG000041777.1) HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0 Identity = 346/347 (99.71%), Postives = 346/347 (99.71%), Query Frame = 1
Query: 52 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQA 1092
MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQ
Sbjct: 1 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQG 347
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Match: SMESG000041777.1 (SMESG000041777.1) HSP 1 Score: 712.99 bits (1839), Expect = 0.000e+0 Identity = 347/350 (99.14%), Postives = 347/350 (99.14%), Query Frame = 1
Query: 52 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQL 1101
MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQ L
Sbjct: 1 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQGYGL 350
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Match: SMESG000041777.1 (SMESG000041777.1) HSP 1 Score: 712.99 bits (1839), Expect = 0.000e+0 Identity = 346/350 (98.86%), Postives = 348/350 (99.43%), Query Frame = 1
Query: 52 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQL 1101
MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQ ++
Sbjct: 1 MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQGCEV 350 HSP 2 Score: 84.7297 bits (208), Expect = 1.326e-16 Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 2
Query: 1307 GCEVSIPTYNCDSKQYSSHLHVQKPKALSMVDHSWPG 1417
GCEVSIPTYNCDSKQYSSHLHVQKPKALSMVDHSWPG
Sbjct: 347 GCEVSIPTYNCDSKQYSSHLHVQKPKALSMVDHSWPG 383
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Match: SMESG000041780.1 (SMESG000041780.1) HSP 1 Score: 353.599 bits (906), Expect = 1.461e-110 Identity = 169/339 (49.85%), Postives = 236/339 (69.62%), Query Frame = 1
Query: 106 ARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQAIQLVNQNGKS 1122
AR+II+S + KK+T AE + ++C + + +A IDD R Y +SDVK + +IASALTK GV +GT+AGII++NSPEY++LIYALSS+GC VT CN N+T E+ QL S PSI+F ++ECL KV+ S+LS VK+IY+IG+ +PGY++V+ LM DSGNM+PIGQ+ L+LD +A++PYSSGT+GLPKGVMLTH+N V++ +Q+ HE + ++ LP+YH+Y + I + F G V Q KF+P L AIE+YK++VLHL+PP +LFLLN+ + F+L+S+ K AA LD +ENR IE F+ P+F T Q L G S
Sbjct: 19 ARSIISSLQPVTNVKKQTTAELIISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAVLHLIPPLVLFLLNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQGYGLTETCGVS 357
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Match: SMESG000064689.1 (SMESG000064689.1) HSP 1 Score: 85.8853 bits (211), Expect = 5.857e-17 Identity = 77/286 (26.92%), Postives = 128/286 (44.76%), Query Frame = 1
Query: 136 NISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKE---CLSKVTNVISELSTVKNIYLIG-----DPLPGYE-TVDKLMADSGNM----NPIGQINLDLDSHIALM-PYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLL 951
N+ + TI + + S++IAF+ +T+SDV + IA+ L K GVK G + + P YV + +S +G + N N + + ++D + S + E L ++ + IS+ T K I++ G PL G + DK++ NM NP ++ H LM ++SGTTGLPK +TH V +++ I + + LPLYH +I + GS M + KF + Y +YK +V+ + +LL
Sbjct: 27 NMKRNRHTIPDIFEKTVQRKSSHIAFL--FEDEQWTFSDVDMYANKIANYLLKLGVKSGDTIALFMESRPAYVAIWMGISKIGAVPALINFNLRKESLTHCINDVKASGLICGTELHSALQEINDDISKFITFK-IFIDGSLIQRKPLNGLTGSADKILDKIINMESIQNPPTTRHITRKVHDPLMFIFTSGTTGLPKAAKVTHARFVMMSYGVSQMLGIQESDIIYNPLPLYHTAGGMIGVGQVIIRGSIMAIRKKFSASAYWSDCIKYKATVVQYIGEICRYLL 309 The following BLAST results are available for this feature:
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase family protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005912 ID=SMED30005912|Name=4-coumarate--CoA ligase family protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3011bpback to top protein sequence of SMED30005912-orf-1 >SMED30005912-orf-1 ID=SMED30005912-orf-1|Name=SMED30005912-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=167bp MHNQPEMHSNEPKKKPEVIKYFYATKAGVDRLDKMVRCYVIKRSTRRWPMback to top protein sequence of SMED30005912-orf-2 >SMED30005912-orf-2 ID=SMED30005912-orf-2|Name=SMED30005912-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=379bp MFVSRNFLKLSFQSVIFHARTIITSKYENISYKKETIAEHVFTFCDKYSNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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