SMED30005911
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005911 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 20
Alignments
SMED30005911 aligns in the following genomic locations:
Homology
BLAST of SMED30005911 vs. Ensembl Fly
Match: cib (gene:FBgn0026084 transcript:FBtr0070639) HSP 1 Score: 58.9214 bits (141), Expect = 5.937e-11 Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = -2 Query: 122 IGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEK--GAPLKHVEVTEKVALPTKEDIEAEKQ 322 I +FN+ +LKH E EK+ LPD A+ QEK+++ IE LKH E EK LPTKE IEAEKQ Sbjct: 60 ITAFNQNNLKHTETNEKNPLPDKEAIEQEKEKNQFIAGIENFDAKKLKHTETNEKNVLPTKEVIEAEKQ 128
BLAST of SMED30005911 vs. Ensembl Fly
Match: cib (gene:FBgn0026084 transcript:FBtr0070641) HSP 1 Score: 58.9214 bits (141), Expect = 5.937e-11 Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = -2 Query: 122 IGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEK--GAPLKHVEVTEKVALPTKEDIEAEKQ 322 I +FN+ +LKH E EK+ LPD A+ QEK+++ IE LKH E EK LPTKE IEAEKQ Sbjct: 60 ITAFNQNNLKHTETNEKNPLPDKEAIEQEKEKNQFIAGIENFDAKKLKHTETNEKNVLPTKEVIEAEKQ 128
BLAST of SMED30005911 vs. Ensembl Fly
Match: cib (gene:FBgn0026084 transcript:FBtr0307206) HSP 1 Score: 58.9214 bits (141), Expect = 5.937e-11 Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = -2 Query: 122 IGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEK--GAPLKHVEVTEKVALPTKEDIEAEKQ 322 I +FN+ +LKH E EK+ LPD A+ QEK+++ IE LKH E EK LPTKE IEAEKQ Sbjct: 60 ITAFNQNNLKHTETNEKNPLPDKEAIEQEKEKNQFIAGIENFDAKKLKHTETNEKNVLPTKEVIEAEKQ 128
BLAST of SMED30005911 vs. Ensembl Fly
Match: cib (gene:FBgn0026084 transcript:FBtr0343425) HSP 1 Score: 58.9214 bits (141), Expect = 5.937e-11 Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = -2 Query: 122 IGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEK--GAPLKHVEVTEKVALPTKEDIEAEKQ 322 I +FN+ +LKH E EK+ LPD A+ QEK+++ IE LKH E EK LPTKE IEAEKQ Sbjct: 60 ITAFNQNNLKHTETNEKNPLPDKEAIEQEKEKNQFIAGIENFDAKKLKHTETNEKNVLPTKEVIEAEKQ 128
BLAST of SMED30005911 vs. TrEMBL
Match: A0A3P7MQF8 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS15854 PE=4 SV=1) HSP 1 Score: 64.6994 bits (156), Expect = 4.981e-10 Identity = 45/93 (48.39%), Postives = 57/93 (61.29%), Query Frame = -2 Query: 116 KMSDEVKIDVNEIGSFNKE-SLKHIEVQEKDSLPD-----------AAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEKQHA 358 K D+V DV + F+KE LKH+EV EK LPD A+ +EK E LR+EIE+G LKHV+ TEK LPTK+DIEAEK+ + Sbjct: 5 KSGDKVLADVQK---FDKEKGLKHVEVTEKVVLPDKDGTPFYLIDIFLAIEKEKTEVSLRQEIEQGKDLKHVKTTEKNVLPTKQDIEAEKKSS 94
BLAST of SMED30005911 vs. TrEMBL
Match: C1L942 (Putative beta thymosin OS=Schistosoma japonicum OX=6182 GN=EWB00_001724 PE=2 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 1.008e-9 Identity = 36/77 (46.75%), Postives = 50/77 (64.94%), Query Frame = -2 Query: 125 MSDEVKIDVNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEK 355 M+D +K+ + +I F+K+ L+H+E +EK LPD +A+EK E L +EIE LKH EK LPTK+DI AEK Sbjct: 1 MADAIKV-LEDIDGFDKQKLRHVETEEKVVLPDKEVIAKEKTEKQLLQEIETPPSLKHTSTKEKNPLPTKDDIVAEK 76
BLAST of SMED30005911 vs. TrEMBL
Match: Q5BTJ4 (SJCHGC00690 protein (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=2) HSP 1 Score: 63.5438 bits (153), Expect = 1.242e-9 Identity = 36/77 (46.75%), Postives = 50/77 (64.94%), Query Frame = -2 Query: 125 MSDEVKIDVNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEK 355 M+D +K+ + +I F+K+ L+H+E +EK LPD +A+EK E L +EIE LKH EK LPTK+DI AEK Sbjct: 13 MADAIKV-LEDIDGFDKQKLRHVETEEKVVLPDKEVIAKEKTEKQLLQEIETPPSLKHTSTKEKNPLPTKDDIVAEK 88
BLAST of SMED30005911 vs. TrEMBL
Match: A0A0R3UPZ4 (WH2 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS9935 PE=4 SV=1) HSP 1 Score: 62.3882 bits (150), Expect = 2.623e-9 Identity = 35/68 (51.47%), Postives = 42/68 (61.76%), Query Frame = -2 Query: 122 EIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEKQ 325 E+G F K+ LKH+ K+ LP A + EK E L +IEKG LK VE EK LPTKEDIE EK+ Sbjct: 11 EVGDFQKDKLKHVNPNIKNPLPTADDVKNEKAESQLMHDIEKGTNLKKVETVEKNPLPTKEDIEREKK 78
BLAST of SMED30005911 vs. TrEMBL
Match: A0A267DFY6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016979g1 PE=4 SV=1) HSP 1 Score: 62.3882 bits (150), Expect = 2.889e-9 Identity = 32/61 (52.46%), Postives = 41/61 (67.21%), Query Frame = -2 Query: 149 VNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPT 331 ++++ FN E LK +E +K LP A + QEK+E LREEIEKG LKHVEV EK LP+ Sbjct: 12 LSDVSKFNTEKLKKVESLDKTVLPSAEQVEQEKREVSLREEIEKGTELKHVEVQEKTVLPS 72
BLAST of SMED30005911 vs. Ensembl Nematostella
Match: EDO48924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHW3]) HSP 1 Score: 51.2174 bits (121), Expect = 7.266e-8 Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = -2 Query: 110 MSDEVKIDVNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKK--EHCLREEIEK--GAPLKHVEVTEKVALPTKEDIEAEKQHA*F 355 MS K DV+E+ F+ +LKH++ +EK++LP + QE + E R E++ + L+HVE EK LPTK+ I EK+ A F Sbjct: 1 MSQGSKPDVSEVAKFDAANLKHVQTKEKNTLPSDETIKQELQPDEFPDRAEVKSFEKSKLQHVETKEKNTLPTKDTIADEKRTAPF 86 HSP 2 Score: 49.6766 bits (117), Expect = 2.644e-7 Identity = 32/66 (48.48%), Postives = 39/66 (59.09%), Query Frame = -2 Query: 128 FNKESLKHIEVQEKDSLPDAAALAQEKKEHCL--REEIEK--GAPLKHVEVTEKVALPTKEDIEAE 313 F K LKH+E EK+ LPDA + E L R E+ K + LKHVE EKV +PTK+ IEAE Sbjct: 92 FQKNKLKHVETLEKNPLPDAQNIRAEMMPEVLPDRSEVAKFDTSKLKHVETKEKVVMPTKDVIEAE 157 HSP 3 Score: 45.8246 bits (107), Expect = 4.613e-6 Identity = 30/77 (38.96%), Postives = 40/77 (51.95%), Query Frame = -2 Query: 125 MSDEVKIDVNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEK 355 M EV DV+ + SF+ LKH+EVQEK+ LP + E E + + LKHV+ EK LP + I EK Sbjct: 270 MRPEVLPDVSAVASFDASKLKHVEVQEKNPLPTKDDITTESTETRAEVKTFDHSKLKHVQTEEKNPLPDAKTIAQEK 346
BLAST of SMED30005911 vs. Planmine SMEST
Match: SMESG000016237.1 (SMESG000016237.1) HSP 1 Score: 77.411 bits (189), Expect = 1.550e-18 Identity = 44/80 (55.00%), Postives = 54/80 (67.50%), Query Frame = -2 Query: 116 MSDEVKIDVNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEKQHA 355 MSD K+ ++EI SF+K+ LKH+E EK+ LPDA + K L EIEKG LKHVE EK LPTKE+IEAEK +A Sbjct: 1 MSDSTKVPIDEIESFDKDKLKHVETVEKEVLPDAEEFNKIKTGKELHSEIEKGTALKHVETEEKSKLPTKEEIEAEKLNA 80
BLAST of SMED30005911 vs. Planmine SMEST
Match: SMESG000025889.1 (SMESG000025889.1) HSP 1 Score: 77.0258 bits (188), Expect = 5.146e-18 Identity = 41/70 (58.57%), Postives = 49/70 (70.00%), Query Frame = -2 Query: 122 VNEIGSFNKESLKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEKQ 331 VNE+ F K+ LKH+E EK LPD + QEK E L++EIEKGA LKHVE TEK LP K+DI+ EKQ Sbjct: 15 VNEVHDFPKDKLKHVEALEKVVLPDNDTIKQEKTEQELKDEIEKGAKLKHVETTEKTVLPNKDDIKKEKQ 84
BLAST of SMED30005911 vs. Planmine SMEST
Match: SMESG000025888.1 (SMESG000025888.1) HSP 1 Score: 54.6842 bits (130), Expect = 2.419e-9 Identity = 30/61 (49.18%), Postives = 37/61 (60.66%), Query Frame = -2 Query: 116 LKHIEVQEKDSLPDAAALAQEKKEHCLREEIEKGAPLKHVEVTEKVALPTKEDIEAEKQHA 298 LK +EK LP+ + QEK E LR+EIEKG PLKH EKV LP + DI EK+ + Sbjct: 50 LKQTNTKEKIILPNQDMIEQEKTEIQLRDEIEKGKPLKHTNTMEKVVLPNQHDIAKEKRES 110 The following BLAST results are available for this feature:
BLAST of SMED30005911 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 4
BLAST of SMED30005911 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005911 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005911 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30005911 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005911 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30005911 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005911 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005911 ID=SMED30005911|Name=SMED30005911|organism=Schmidtea mediterranea sexual|type=transcript|length=677bpback to top Annotated Terms
The following terms have been associated with this transcript:
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