Atg13

Overview
NameAtg13
Smed IDSMED30005891
Length (bp)1556
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Atg13 (SMED30005891) t-SNE clustered cells

Violin plots show distribution of expression levels for Atg13 (SMED30005891) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Atg13 (SMED30005891) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Atg13 (SMED30005891) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005891

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30005891h1SMcG0000910 dd_Smed_v4_4306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30005891h1SMcG0000910 dd_Smed_v4_4306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30005891h1SMcG0000910 Contig36079uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30005891h1SMcG0000910 Contig36079newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30005891h1SMcG0000910 Contig36079uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30005891h1SMcG0000910 Contig36079newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30005891h1SMcG0000910 dd_Smed_v4_4306_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Atg13 vs. Ensembl Fly
Match: Atg13 (gene:FBgn0261108 transcript:FBtr0081843)

HSP 1 Score: 56.9954 bits (136), Expect = 2.707e-8
Identity = 50/171 (29.24%), Postives = 84/171 (49.12%), Query Frame = 2 
Query:  101 LEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLD--SGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSV---ESTTEPNSN-LMGNIADLKKTRFL-ERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLG 586
            LEK  +  ++K  Q +VQ+R G    T C    G+ +   + ++D PE+    K++L+  +G S++      VEI  K   G+++ +LEVW   +   ++   P +N L      LK    +  R+G +LKS++  TR  PAYK SR    +   + Y +    P +  LG
Sbjct:   13 LEKFIKFLVLKSTQVVVQSRLGEKMQTQCNPLAGS-DWFNIAVQDHPEVLDETKRALNLKTGESILQRLPLCVEISLKTTEGDQM-VLEVWSLDLLQPQNGASPATNDLNPEGQTLKAAHAIYNRMGIMLKSLISLTRTTPAYKLSRRQCPDSYGIFYRIYVDRPQVHTLG 181
[Close]
BLAST of Atg13 vs. UniProt/SwissProt
Match: sp|Q9VHR6|ATG13_DROME (Autophagy-related protein 13 homolog OS=Drosophila melanogaster OX=7227 GN=Atg13 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.714e-7
Identity = 50/171 (29.24%), Postives = 84/171 (49.12%), Query Frame = 2 
Query:  101 LEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLD--SGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSV---ESTTEPNSN-LMGNIADLKKTRFL-ERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLG 586
            LEK  +  ++K  Q +VQ+R G    T C    G+ +   + ++D PE+    K++L+  +G S++      VEI  K   G+++ +LEVW   +   ++   P +N L      LK    +  R+G +LKS++  TR  PAYK SR    +   + Y +    P +  LG
Sbjct:   13 LEKFIKFLVLKSTQVVVQSRLGEKMQTQCNPLAGS-DWFNIAVQDHPEVLDETKRALNLKTGESILQRLPLCVEISLKTTEGDQM-VLEVWSLDLLQPQNGASPATNDLNPEGQTLKAAHAIYNRMGIMLKSLISLTRTTPAYKLSRRQCPDSYGIFYRIYVDRPQVHTLG 181
[Close]
BLAST of Atg13 vs. TrEMBL
Match: A0A2S1BJJ9 (Atg13 OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0
Identity = 360/402 (89.55%), Postives = 376/402 (93.53%), Query Frame = 2 
Query:   65 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGNELPLLEVWHFSVESTTEPNSNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQXXXXXXXXXXXXXXALKVYTHYRSHEGIRLLREKQTVYLENNNKKVEDNKIKADTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEKLCMEFDKKIDKFEMKDGYKXXXXXXXXXXXXXXXXPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDSGRIDISCMKDELLQHEKLSVEFDDFLHLFEEGRAGKTVPAID 1270
            MINNR PDEISALEKCFRPFIIKCIQSIVQARSGN+YNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVG++LPLLEVWHFSVESTTEPN+N++GN+ADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNL NLG HCQGRSVGSLVVPSSGVALKVYTHYRSHEGIRLLREKQT  +E NNKKVED+K K DTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEK+CMEFD K DK ++KDGYK S +     +     IPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDS RIDISCMKD+LLQHEKLSVEFDDFL LFEEGR GK+VPAID
Sbjct:    1 MINNRAPDEISALEKCFRPFIIKCIQSIVQARSGNIYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGSDLPLLEVWHFSVESTTEPNTNMLGNLADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLTNLGAHCQGRSVGSLVVPSSGVALKVYTHYRSHEGIRLLREKQTALMETNNKKVEDSKTKIDTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEKICMEFDNKFDKIDLKDGYK-SSDNEGDTDVESNGIPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDSTRIDISCMKDDLLQHEKLSVEFDDFLQLFEEGRTGKSVPAID 401
[Close]
BLAST of Atg13 vs. TrEMBL
Match: U6J416 (Cell adhesion molecule OS=Echinococcus granulosus OX=6210 GN=EGR_10405 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 6.415e-43
Identity = 93/214 (43.46%), Postives = 125/214 (58.41%), Query Frame = 2 
Query:   83 PDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVG-NELPLLEVWHFSVESTTE-------PNSNLMGN-------------------------------------IADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGN 589
            P+E +A+E+C R FIIK IQ+IVQARSG  Y T+C+SDVGARNN+MVY+E+DPEIGATIKQ+LDSGF +  GD F++EI    G   L +LEVW F +++          P S ++ N                                     + D +++R  ERL +LLKS++VTTR+LPAY  SR P    + MCY +RRG  +L  LG+
Sbjct:    6 PEETAAIERCIRSFIIKSIQAIVQARSGAAYATTCISDVGARNNLMVYLEEDPEIGATIKQNLDSGFPIKMGDTFSLEILMSNGAGGLVVLEVWQFRLDTEDNLLRRGHLPCSKVIANHSGLQPPIYFPTVNIPTASSAATAVSDATIAAPHCFAVEDFRRSRLFERLASLLKSLLVTTRMLPAYTYSRKP---QHEMCYALRRGEVSLDRLGH 216
[Close]
BLAST of Atg13 vs. TrEMBL
Match: A0A068Y8V0 (Cell adhesion molecule OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000884200 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 6.973e-43
Identity = 93/214 (43.46%), Postives = 125/214 (58.41%), Query Frame = 2 
Query:   83 PDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVG-NELPLLEVWHFSVESTTE-------PNSNLMGN-------------------------------------IADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGN 589
            P+E +A+E+C R FIIK IQ+IVQARSG  Y T+C+SDVGARNN+MVY+E+DPEIGATIKQ+LDSGF +  GD F++EI    G   L +LEVW F +++          P S ++ N                                     + D +++R  ERL +LLKS++VTTR+LPAY  SR P    + MCY +RRG  +L  LG+
Sbjct:    6 PEETAAIERCIRSFIIKSIQAIVQARSGAAYATTCISDVGARNNLMVYLEEDPEIGATIKQNLDSGFPIKMGDTFSLEILMSNGAGGLVVLEVWQFRLDTEDNLLRRGHLPCSKVIANHSGLQPPIYFPTVNSPTASIAATAVSDATIAAPHCFAVEDFRRSRLFERLASLLKSLLVTTRMLPAYTYSRKP---QHEMCYALRRGEVSLDRLGH 216
[Close]
BLAST of Atg13 vs. TrEMBL
Match: A0A0R3X4U3 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS8433 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 4.952e-41
Identity = 91/214 (42.52%), Postives = 121/214 (56.54%), Query Frame = 2 
Query:   83 PDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVG-NELPLLEVWHFSVESTTE-------PNSNLMGN-------------------------------------IADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGN 589
            P+E  A+E+C R FIIK IQ+IVQARSG  Y T+C+SDVGARNN+MVY+E+DPEIGATIKQ+LDSGF +  GD F++EI    G   L +LEVW F +++          P S ++ N                                     + D +++R  ERL +LLKS++VTTR+LPAY  SR      + MCY +RRG  +   LG 
Sbjct:    6 PEERVAIERCIRSFIIKSIQAIVQARSGTAYATACISDVGARNNLMVYLEEDPEIGATIKQNLDSGFPIKMGDTFSLEILMSNGAGRLVVLEVWQFRLDTEDNLLQRAHLPCSKVVANHSGLQPPIYFPTVTSPTVSSSATGVSDATIVAPQCFAVEDFRRSRLFERLASLLKSLLVTTRMLPAYTYSRK---QQHEMCYTLRRGEVSFDRLGQ 216
[Close]
BLAST of Atg13 vs. TrEMBL
Match: A0A0R3W6F6 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS5777 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 5.344e-41
Identity = 92/220 (41.82%), Postives = 125/220 (56.82%), Query Frame = 2 
Query:   65 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVG-NELPLLEVWHFSVESTTE-------PNSNLMGN-------------------------------------IADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGN 589
            M ++R P+E  ++E+C R FIIK IQ+IVQARSG  Y T+C+SDVGARNN+MVY+E+DPEIGATIKQ+LDSGF +  GD F++EI    G   L +LEVW F +++          P S ++ N                                     + D +++R  ERL +LLKS++VTTR+LPAY  SR      + MCY +RRG  +   LG 
Sbjct:    1 MASSR-PEETLSIERCIRSFIIKSIQAIVQARSGTAYATTCISDVGARNNLMVYLEEDPEIGATIKQNLDSGFPIKMGDTFSLEILMSNGAGRLVVLEVWQFRLDTEDNLLQRAHLPCSKVVANHSGLQSPIYFPTVTGPTASSVATGVSDATVIAPQCFAVEDFRRSRLFERLASLLKSLIVTTRMLPAYTYSRK---QQHEMCYTLRRGEVSFDRLGQ 216
[Close]
BLAST of Atg13 vs. Ensembl Medaka
Match: atg13 (autophagy related 13 [Source:NCBI gene;Acc:101158003])

HSP 1 Score: 56.6102 bits (135), Expect = 2.089e-8
Identity = 51/174 (29.31%), Postives = 78/174 (44.83%), Query Frame = 2 
Query:   83 PDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSVESTTEPNSNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQ 598
            P +   L+K  + F +K +Q IVQAR G    T   S     +   + I+D PE+    K++L      + G +  VEI  K   G+ +  LE W   +    + +  +   + +        RL  LLKS+M  TR+ PAYK SR    +D  + Y +  G   L+ LG   Q
Sbjct:    7 PQDKKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVSHEAKKALAGQLPGI-GRSMCVEISLKTSEGDSME-LETWCLEMNEKCDKDIKVSYTVYN--------RLSVLLKSLMAITRVTPAYKLSRKQ-GHDYVILYRIYFGEVQLSGLGEGFQ 169
[Close]
BLAST of Atg13 vs. Ensembl Medaka
Match: atg13 (autophagy related 13 [Source:NCBI gene;Acc:101158003])

HSP 1 Score: 54.299 bits (129), Expect = 1.961e-7
Identity = 54/181 (29.83%), Postives = 82/181 (45.30%), Query Frame = 2 
Query:   65 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSVESTTEPNSNLMGNIADLKKTRFL-ERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQ 598
            M ++  P +   L+K  + F +K +Q IVQAR G    T   S     +   + I+D PE+    K++L      + G +  VEI  K   G+ +  LE W   +    +          D+K +  +  RL  LLKS+M  TR+ PAYK SR    +D  + Y +  G   L+ LG   Q
Sbjct:    1 MDSDLSPQDKKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVSHEAKKALAGQLPGI-GRSMCVEISLKTSEGDSME-LETWCLEMNEKCD---------KDIKVSYTVYNRLSVLLKSLMAITRVTPAYKLSRK-QGHDYVILYRIYFGEVQLSGLGEGFQ 169
[Close]
BLAST of Atg13 vs. Ensembl Medaka
Match: atg13 (autophagy related 13 [Source:NCBI gene;Acc:101158003])

HSP 1 Score: 54.299 bits (129), Expect = 1.961e-7
Identity = 54/181 (29.83%), Postives = 82/181 (45.30%), Query Frame = 2 
Query:   65 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSVESTTEPNSNLMGNIADLKKTRFL-ERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQ 598
            M ++  P +   L+K  + F +K +Q IVQAR G    T   S     +   + I+D PE+    K++L      + G +  VEI  K   G+ +  LE W   +    +          D+K +  +  RL  LLKS+M  TR+ PAYK SR    +D  + Y +  G   L+ LG   Q
Sbjct:    1 MDSDLSPQDKKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVSHEAKKALAGQLPGI-GRSMCVEISLKTSEGDSME-LETWCLEMNEKCD---------KDIKVSYTVYNRLSVLLKSLMAITRVTPAYKLSRK-QGHDYVILYRIYFGEVQLSGLGEGFQ 169
[Close]
BLAST of Atg13 vs. Ensembl Medaka
Match: atg13 (autophagy related 13 [Source:NCBI gene;Acc:101158003])

HSP 1 Score: 52.7582 bits (125), Expect = 5.746e-7
Identity = 53/175 (30.29%), Postives = 78/175 (44.57%), Query Frame = 2 
Query:   83 PDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKV--GNELPLLEVWHFSVESTTEPNSNLMGNIADLKKT-RFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQ 598
            P +   L+K  + F +K +Q IVQAR G    T   S     +   + I+D PE+    K++L      + G +  VEI  K   G+ +  LE W   +    +          D+K +     RL  LLKS+M  TR+ PAYK SR    +D  + Y +  G   L+ LG   Q
Sbjct:    7 PQDKKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVSHEAKKALAGQLPGI-GRSMCVEISLKTSEGDSME-LETWCLEMNEKCD---------KDIKVSYTVYNRLSVLLKSLMAITRVTPAYKLSRK-QGHDYVILYRIYFGEVQLSGLGEGFQ 169
[Close]
BLAST of Atg13 vs. Planmine SMEST
Match: SMESG000029174.1 (SMESG000029174.1)

HSP 1 Score: 763.451 bits (1970), Expect = 0.000e+0
Identity = 401/402 (99.75%), Postives = 401/402 (99.75%), Query Frame = 2 
Query:   65 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGNELPLLEVWHFSVESTTEPNSNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQXXXXXXXXXXXXXXALKVYTHYRSHEGIRLLREKQTVYLENNNKKVEDNKIKADTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEKLCMEFDKKIDKFEMKDGYKXXXXXXXXXXXXXXXXPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDSGRIDISCMKDELLQHEKLSVEFDDFLHLFEEGRAGKTVPAID 1270
            MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGNELPLLEVWHFSVESTTEP SNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQGRSVGSLVVPSSGVALKVYTHYRSHEGIRLLREKQTVYLENNNKKVEDNKIKADTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEKLCMEFDKKIDKFEMKDGYKSSDNEIDSDNDSDAAIPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDSGRIDISCMKDELLQHEKLSVEFDDFLHLFEEGRAGKTVPAID
Sbjct:    1 MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNNMMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGNELPLLEVWHFSVESTTEPISNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSRNPITNDNPMCYLVRRGAPNLANLGNHCQGRSVGSLVVPSSGVALKVYTHYRSHEGIRLLREKQTVYLENNNKKVEDNKIKADTKRSQIPIKKNRPAFATNYVDYHDDDEDEIEKLCMEFDKKIDKFEMKDGYKSSDNEIDSDNDSDAAIPRKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDLDMFATDMSDSGRIDISCMKDELLQHEKLSVEFDDFLHLFEEGRAGKTVPAID 402
The following BLAST results are available for this feature:
BLAST of Atg13 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Atg132.707e-829.24gene:FBgn0261108 transcript:FBtr0081843[more]
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BLAST of Atg13 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9VHR6|ATG13_DROME2.714e-729.24Autophagy-related protein 13 homolog OS=Drosophila... [more]
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BLAST of Atg13 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2S1BJJ90.000e+089.55Atg13 OS=Dugesia japonica OX=6161 PE=2 SV=1[more]
U6J4166.415e-4343.46Cell adhesion molecule OS=Echinococcus granulosus ... [more]
A0A068Y8V06.973e-4343.46Cell adhesion molecule OS=Echinococcus multilocula... [more]
A0A0R3X4U34.952e-4142.52Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
A0A0R3W6F65.344e-4141.82Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
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BLAST of Atg13 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Atg13 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
atg132.089e-829.31autophagy related 13 [Source:NCBI gene;Acc:1011580... [more]
atg131.961e-729.83autophagy related 13 [Source:NCBI gene;Acc:1011580... [more]
atg131.961e-729.83autophagy related 13 [Source:NCBI gene;Acc:1011580... [more]
atg135.746e-730.29autophagy related 13 [Source:NCBI gene;Acc:1011580... [more]
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BLAST of Atg13 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000029174.10.000e+099.75SMESG000029174.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005891 ID=SMED30005891|Name=Atg13|organism=Schmidtea mediterranea sexual|type=transcript|length=1556bp
TCTGTTTAAATATTAACACGTTTTTCACACAATTTTAAATTAATAAATAT
TCCCGCGCTTAAAAATGATTAATAATCGAGTACCCGATGAAATAAGTGCT
CTTGAAAAGTGTTTTAGACCATTTATTATTAAATGTATTCAATCTATAGT
GCAAGCGCGAAGTGGCAACGTTTATAATACAAGTTGCATGTCTGATGTTG
GCGCTAGAAATAATATGATGGTTTATATTGAGGATGACCCAGAAATAGGT
GCAACAATTAAGCAAAGTCTTGATTCTGGATTTTCTGTTGTTCCTGGTGA
CAATTTTACTGTTGAAATTTACGGGAAAGTAGGAAATGAATTACCACTAT
TAGAAGTGTGGCATTTTTCAGTTGAATCAACAACAGAACCTAATTCTAAT
TTAATGGGTAATATTGCCGATTTAAAGAAAACAAGATTTCTAGAGCGTTT
GGGTACGCTTTTAAAGTCCATTATGGTTACAACAAGACTTCTTCCTGCAT
ATAAAAATTCTCGCAATCCAATAACTAATGATAATCCAATGTGTTATTTA
GTGAGAAGAGGTGCTCCAAATTTAGCTAATTTGGGTAATCACTGTCAAGG
TCGAAGTGTTGGGAGTTTAGTTGTACCATCGTCCGGAGTAGCTTTGAAAG
TCTATACTCATTATAGATCTCATGAAGGAATACGTTTGTTGAGAGAGAAA
CAAACTGTATATTTGGAAAATAATAACAAAAAGGTGGAAGATAACAAAAT
CAAAGCTGATACTAAAAGATCTCAAATACCAATCAAGAAAAATCGACCAG
CTTTCGCTACTAATTACGTTGATTATCATGATGATGATGAAGATGAAATT
GAAAAGTTATGCATGGAATTTGACAAAAAAATCGATAAATTTGAAATGAA
AGACGGCTATAAAAGTAGTGACAATGAAATAGACTCTGATAATGATTCTG
ATGCTGCAATTCCAAGAAAAACCAGATCTCAAGCTCTTTTGACAGCGGCT
ATGTCAACTAGTCCTGAAGAAGTGAAATATCGACTGCAGTTACCATTCAG
TAATTTATCAACTGGTGCTGACTACAGTCGAATGTTTACAGATCTGAAAT
TGAGAGGAGATCTCGATATGTTTGCTACAGACATGTCGGATTCGGGTCGG
ATTGACATAAGTTGTATGAAAGATGAATTATTACAGCATGAAAAATTGTC
TGTTGAATTCGATGATTTCTTGCATTTGTTTGAGGAAGGTCGAGCAGGTA
AAACAGTTCCAGCTATCGATTGAAAGTGAGTGAGGCAAACATATATGTAT
TTAAATATCACAATCAATCAATCAATTATTCAAAGGTTTTAACTATTTTT
CTTCGGTGAAATTTTGACCAACTTATAAAATATTGATTTTACCAATTCTG
TAAATATATTTCTATAAGGTCTGCATTGCAACTTTACTGGCACCCGTTTA
TCACTTTATTCCAGGTGTAAATTTTATCAATTCATCTATTTGAATATAAA
AATCTATTAGACAAATAATTTCTTCAATTTCTTTGCCAAAAAAAAACTGA
TATTAT
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protein sequence of SMED30005891-orf-1

>SMED30005891-orf-1 ID=SMED30005891-orf-1|Name=SMED30005891-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=403bp
MINNRVPDEISALEKCFRPFIIKCIQSIVQARSGNVYNTSCMSDVGARNN
MMVYIEDDPEIGATIKQSLDSGFSVVPGDNFTVEIYGKVGNELPLLEVWH
FSVESTTEPNSNLMGNIADLKKTRFLERLGTLLKSIMVTTRLLPAYKNSR
NPITNDNPMCYLVRRGAPNLANLGNHCQGRSVGSLVVPSSGVALKVYTHY
RSHEGIRLLREKQTVYLENNNKKVEDNKIKADTKRSQIPIKKNRPAFATN
YVDYHDDDEDEIEKLCMEFDKKIDKFEMKDGYKSSDNEIDSDNDSDAAIP
RKTRSQALLTAAMSTSPEEVKYRLQLPFSNLSTGADYSRMFTDLKLRGDL
DMFATDMSDSGRIDISCMKDELLQHEKLSVEFDDFLHLFEEGRAGKTVPA
ID*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000136whole organism
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036570HORMA_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006914autophagy
Vocabulary: cellular component
TermDefinition
GO:1990316ATG1/ULK1 kinase complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036570HORMA domain superfamilyGENE3DG3DSA:3.30.900.10coord: 10..207
e-value: 2.2E-34
score: 120.9