Neurotrypsin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005872 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14
Homology
BLAST of Neurotrypsin vs. Ensembl Human
Match: PRSS12 (serine protease 12 [Source:HGNC Symbol;Acc:HGNC:9477]) HSP 1 Score: 311.227 bits (796), Expect = 1.414e-89 Identity = 192/559 (34.35%), Postives = 289/559 (51.70%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKN---RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQVNG-IYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
KS G ++CG D ++C +T IRL G K EG++E+ Y+G+WG +CDD +T +V+C+QLGF + S F + PI LD++ C G E +C W +H+C E D + C + V+L++G + GRVE+ + GT+CDDG+TD +A+VICR LG G A+A T FG G G I + N +C GNE SL C G H C H EDAGVIC + S + SL CG + + +R+ R++GG ++ G +PWQV +R + D CG ++LS W+++A+HC+++ N Y VR GD++ + +E+ ++++ ++ +Y+ P D+D AL++++ F+ HV P CLP P + C ++GWGDT + L A +PL+ C+E YK R T RM CAG +E +D+CQGDSGGPL+C+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 361 KSSWGEHNCGHKEDAGVSCTPLT--DGVIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQ---ASANHFEESTGPI-WLDDVSCSGKETRFLQCSRRQWGRHDCSHRE--------------------------------DVSIACYPGGEGHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKGPIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSS-------VCGLRLLHRRQKRIIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRSYAVRVGDYHTLVPEEFEEEIGVQQIVIHREYR-PDRSDYDIALVRLQGPEEQCARFSSHVLPACLPLWRERPQKTASNCYITGWGDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWVVYGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVT 873 HSP 2 Score: 151.369 bits (381), Expect = 1.849e-36 Identity = 90/255 (35.29%), Postives = 128/255 (50.20%), Query Frame = 2
Query: 2069 TFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
TFP IRL G + EG+VE+ + G+WG +CDD++ ++A VIC+QLG + H + FG+ P+ +LDE++C G E SIE+CP W +HNC +E AGV C + + DG ++L G S+ GR+E+ GTVCDDG+T+ V+CR LG QA + F G I L + C G ET +QC+ +WG H CSH+ED + C
Sbjct: 276 TFPI--IRLAGGSSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQA--YFGEGSGPV-MLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------TPLTDG-----------------VIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQASAN-HFEESTGPIWLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVSIAC 485 HSP 3 Score: 142.124 bits (357), Expect = 1.308e-33 Identity = 87/253 (34.39%), Postives = 117/253 (46.25%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVK----FGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G KN++EG VE+ G WG +C + S+A VIC QL + G G+ F + I ++C+G E +I C D+W+ C + A V C G F ++L G + GRVE+ + GTVCDD + D++A VICR LG+SG A+A FG G G ++L RC GNE S+ QC WGEH C HKEDAGV C
Sbjct: 170 VRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQL-------QLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTCSFS------------------------------------HGPTFPIIRLAGGSSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQAYFGEGSGPVMLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC 379 HSP 4 Score: 67.0106 bits (162), Expect = 2.169e-10 Identity = 39/101 (38.61%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
SV+L G + + G VE+ S GTVC + DS+ASVIC L + G+ A G G+ I SN RCRG+E +++ C W C K A V C
Sbjct: 169 SVRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQLQLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTC 269 HSP 5 Score: 65.855 bits (159), Expect = 5.162e-10 Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G S+ +EGRVE+Y++ +WG VCDD W+ DA V C+QLG A+ A Q + G G ++D+++C G+E SI +C
Sbjct: 280 IRLAGGSSVHEGRVELYHAG--QWGTVCDDQWDDADAEVICRQLGLSGIAK-AWHQAYFGEG---SGPVMLDEVRCTGNELSIEQC 359 HSP 6 Score: 55.4546 bits (132), Expect = 6.714e-7 Identity = 32/76 (42.11%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+ + +WG +CDD W DA V C+QLG+ AR T + G G K +D +KC G+E S+ +C
Sbjct: 510 EGRVEVFING--QWGTICDDGWTDKDAAVICRQLGYKGPARART-MAYFGEG---KGPIHVDNVKCTGNERSLADC 579
BLAST of Neurotrypsin vs. Ensembl Human
Match: TMPRSS2 (transmembrane serine protease 2 [Source:HGNC Symbol;Acc:HGNC:11876]) HSP 1 Score: 192.971 bits (489), Expect = 4.257e-52 Identity = 145/431 (33.64%), Postives = 211/431 (48.96%), Query Frame = 2
Query: 2381 AGVICK-VDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNR--YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT--LVETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
AG++ K + C+N+ C TCI S CDG C DE +C V+L GP+ QR S VC D + ++ CR +G F S G + S TS ++ N+ AG + L HS A S K + + CG + R+ R+VGG + GA+PWQV + + ++H CGGSI++ +WI++A+HC EKP N ++ Y +++E++ +P Y T + D AL+K+++ FND V+PVCLP PGM+ + C +SGWG T +T+E L AA+V LI+ +RC + Y + IT M CAG+ G +D+CQGDSGGPLV N I+ + G TSWG GCA PGVY V D++ +
Sbjct: 102 AGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDE--NRC---VRLY-GPNFILQVYSSQRK-SWHPVCQDDWNENYGRAACRDMGY--------KNNFYSSQGIVDDSG------STSFMKLNT------------SAGNVDIYKKLYHS---DACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQ----NVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSK-TKNNDIALMKLQKPLT----FNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQ 487
BLAST of Neurotrypsin vs. Ensembl Human
Match: TMPRSS2 (transmembrane serine protease 2 [Source:HGNC Symbol;Acc:HGNC:11876]) HSP 1 Score: 192.586 bits (488), Expect = 5.856e-52 Identity = 145/431 (33.64%), Postives = 211/431 (48.96%), Query Frame = 2
Query: 2381 AGVICK-VDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNR--YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT--LVETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
AG++ K + C+N+ C TCI S CDG C DE +C V+L GP+ QR S VC D + ++ CR +G F S G + S TS ++ N+ AG + L HS A S K + + CG + R+ R+VGG + GA+PWQV + + ++H CGGSI++ +WI++A+HC EKP N ++ Y +++E++ +P Y T + D AL+K+++ FND V+PVCLP PGM+ + C +SGWG T +T+E L AA+V LI+ +RC + Y + IT M CAG+ G +D+CQGDSGGPLV N I+ + G TSWG GCA PGVY V D++ +
Sbjct: 102 AGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDE--NRC---VRLY-GPNFILQVYSSQRK-SWHPVCQDDWNENYGRAACRDMGY--------KNNFYSSQGIVDDSG------STSFMKLNT------------SAGNVDIYKKLYHS---DACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQ----NVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSK-TKNNDIALMKLQKPLT----FNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQ 487
BLAST of Neurotrypsin vs. Ensembl Human
Match: TMPRSS2 (transmembrane serine protease 2 [Source:HGNC Symbol;Acc:HGNC:11876]) HSP 1 Score: 192.586 bits (488), Expect = 5.856e-52 Identity = 145/431 (33.64%), Postives = 211/431 (48.96%), Query Frame = 2
Query: 2381 AGVICK-VDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNR--YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT--LVETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
AG++ K + C+N+ C TCI S CDG C DE +C V+L GP+ QR S VC D + ++ CR +G F S G + S TS ++ N+ AG + L HS A S K + + CG + R+ R+VGG + GA+PWQV + + ++H CGGSI++ +WI++A+HC EKP N ++ Y +++E++ +P Y T + D AL+K+++ FND V+PVCLP PGM+ + C +SGWG T +T+E L AA+V LI+ +RC + Y + IT M CAG+ G +D+CQGDSGGPLV N I+ + G TSWG GCA PGVY V D++ +
Sbjct: 102 AGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDE--NRC---VRLY-GPNFILQVYSSQRK-SWHPVCQDDWNENYGRAACRDMGY--------KNNFYSSQGIVDDSG------STSFMKLNT------------SAGNVDIYKKLYHS---DACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQ----NVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSK-TKNNDIALMKLQKPLT----FNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQ 487
BLAST of Neurotrypsin vs. Ensembl Human
Match: TMPRSS2 (transmembrane serine protease 2 [Source:HGNC Symbol;Acc:HGNC:11876]) HSP 1 Score: 192.586 bits (488), Expect = 1.325e-51 Identity = 145/431 (33.64%), Postives = 211/431 (48.96%), Query Frame = 2
Query: 2381 AGVICK-VDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNR--YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT--LVETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
AG++ K + C+N+ C TCI S CDG C DE +C V+L GP+ QR S VC D + ++ CR +G F S G + S TS ++ N+ AG + L HS A S K + + CG + R+ R+VGG + GA+PWQV + + ++H CGGSI++ +WI++A+HC EKP N ++ Y +++E++ +P Y T + D AL+K+++ FND V+PVCLP PGM+ + C +SGWG T +T+E L AA+V LI+ +RC + Y + IT M CAG+ G +D+CQGDSGGPLV N I+ + G TSWG GCA PGVY V D++ +
Sbjct: 139 AGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDE--NRC---VRLY-GPNFILQVYSSQRK-SWHPVCQDDWNENYGRAACRDMGY--------KNNFYSSQGIVDDSG------STSFMKLNT------------SAGNVDIYKKLYHS---DACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQ----NVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSK-TKNNDIALMKLQKPLT----FNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQ 524
BLAST of Neurotrypsin vs. Ensembl Celegans
Match: svh-1 (HGF/MSP/plasminogen-like protein [Source:UniProtKB/TrEMBL;Acc:Q17800]) HSP 1 Score: 197.208 bits (500), Expect = 2.386e-51 Identity = 143/438 (32.65%), Postives = 212/438 (48.40%), Query Frame = 2
Query: 2351 RKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFL-SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPK--NRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERC---KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQV-NGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
R+ NC S I+ C+ R C++ + D +CI+ Q+C+G++DC +S E E C S +L NG D+ SG +E+ +C D ++ IC + + G + T G V + C +S C + + CGL +E + R R RVVGGFETV GAFPW +R++ H CG SIL + +I+A+HC+E+ + + Y V GDW+NN+ D EQ F ++ + P YK F D A+++I FN++ QP+CLP V +C+VSGWG + AE+L AA +P+I C + Y ++ CAGY GGID+CQGDSGGP C+ +G + + G SWGDGCA + PG+Y V L ++
Sbjct: 541 REPNCTSTVIS--TCEPIR-CSHCQQAACGDGSCIRFDQLCNGQIDC--QSGEDEDYCRANSFRLTNGSDT-SGYLEVLFRSRWEPICADHLNGKISNSICDAMRL-GEHGFILSTNATISSGYNVNCDVDCSIRRSS--SCTRHARISCISQNGMSSASQCGLRYVEVN-------------------ARDAAKSRIARVVGGFETVPGAFPWTAALRNKATK--AHHCGASILDKTHLITAAHCFEEDERVSSYEVVVGDWDNNQTDGNEQIFYLQRIHFYPLYKD--IFSHDIAILEIPYPGIE---FNEYAQPICLPSKDFVYTPGRQCVVSGWGSMGLRYAERLQAALIPIINRFDCVNSSQIYSS-MSRSAFCAGYLEGGIDSCQGDSGGPFACRREDGAFVLAGVISWGDGCAQKKQPGIYTMVAPYLSWI 942
BLAST of Neurotrypsin vs. Ensembl Celegans
Match: try-1 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q23528]) HSP 1 Score: 124.79 bits (312), Expect = 4.498e-31 Identity = 82/245 (33.47%), Postives = 124/245 (50.61%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPK--NRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYK--GPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTK-CLVSGWGDTL---VETAEKLLAAEVPLIKAERCKEF--YKDRI-TDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVK 3646
R++GG E+ ++PW V + R+G H CGGS++ +++++A+HC+ K + Y VR G + + + ++P Y P ++DF I N +P+CLP +P ++ + C+V+GWG T+ +A L VPL+ C Y RI M CAGY G ID+CQGDSGGPL+C +G + + G SWG GCA P PGVY V S ++
Sbjct: 57 RLIGGSESSPHSWPWTVQLLSRLGH---HRCGGSLIDPNFVLTAAHCFAKDRRPTSYSVRVGGHRSGS----GSPHRVTAVSIHPWYNIGFPSSYDFAIMRIHPPVNTSTT------ARPICLPS---LPAVENRLCVVTGWGSTIEGSSLSAPTLREIHVPLLSTLFCSSLPNYIGRIHLPSMLCAGYSYGKIDSCQGDSGGPLMCARDGHWELTGVVSWGIGCARPGMPGVYGNVHSASTWIN 285
BLAST of Neurotrypsin vs. Ensembl Celegans
Match: lrp-2 (Low-density lipoprotein RecePtor related [Source:UniProtKB/TrEMBL;Acc:O01768]) HSP 1 Score: 78.5666 bits (192), Expect = 2.883e-14 Identity = 63/213 (29.58%), Postives = 91/213 (42.72%), Query Frame = 2
Query: 1427 CADNQFYCP-SMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTG-KYKCSSGMCIALSKSCDGRNDCGDNSDEMNC 2059
C QF C + + +CL S++CD V DCSG +DE P FC+ F C +F C CI +++CD ++ C D +DE NC I + K D F + + +C+ K +F C + P + PDS I +CT +++C+SG CI + CDG DC D SDE C
Sbjct: 3527 CIAGQFVCGRTAQNATAQCLYASKLCDGVKDCSGGDDEEPAFCENFECTEAQFKCGDKKKCIPLTSVCDKEKDCDDGSDELNCEITS-----CKPDFFA----------CFNGTAISKCIPK------EFYCDGEDDCPEGQDE---------------PDSCFGIG----------ECTHEQFQCTSGKCIPKRRQCDGTLDCRDGSDEKGC 3693 HSP 2 Score: 70.0922 bits (170), Expect = 1.130e-11 Identity = 101/412 (24.51%), Postives = 150/412 (36.41%), Query Frame = 2
Query: 1427 CADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRI--FSECHLMKQL---------------QSEIPDSLKHINYPGDHYSLKTDCTGK--YKC-SSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKN--DWEGKVEIRY---QGEWGYICDDRFTI--SEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVIC----------------KVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLS 2533
CA+N+F C ++ +C+ CD+ NDC +DE+ ++C T C+ F C + CI + +C+ C++ ADE NC+ NE E + +P K ++ Q C + GC N NE + C Q S+ D + G S C K + C ++ C+ CDG+ DC D SDE NC + FE SK E K + R GE + C+ A C I G+ + + DE+ C S P + + E+ V+C +DR C E+ C + CI ++CD VDC D SDE C LS
Sbjct: 2639 CAENEFKC----LHSDRCIRYEDTCDRYNDCPMFDDEDTKYCSTRVCRPGYFNC-GNGLCIPEQKVCNRINDCANFADESNCT-CNENEFRCQSGA------CIPSKARCNHM------QDCNDASDEIGCPFRNCSALNEFGMTGLINCATTSQCIHPSWKCDGTNDCYDGSDEKDCFVEFDATGGSVSAPRSCDAKTQFACLATRTCMPKHWQCDGQPDCADGSDEKNCDAKKCTSFEFSCESSKKCIPLEQKCDGRRDCPDGEDEHQCETECDSKGGNATFRCTNHRCIPMAWRCDGTDDCMDNAKS--LGSDEVDCAAGRTSF-HVPSRCADETCVVACELTAVLCDGIQDCSDGFDEKNCASLDRQCKPNEWMCDSGQ-CIDSGRLCDASVDCVDGSDEWVEICSLS 3028 HSP 3 Score: 58.9214 bits (141), Expect = 3.474e-8 Identity = 64/232 (27.59%), Postives = 99/232 (42.67%), Query Frame = 2
Query: 1427 CADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFC-KTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSK--------ILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGM-CIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIR 2092
C+ +QF C KC+ S+ C+ V +C DE E C ++ C +EF C CI +S +CDG+ C D DE++C NE E ++ +F+ ++ D + +E + C+ C +N + + C QL PD L + S+ D T C +G CI+ CDG +DCGDNSDE +C + +R
Sbjct: 1013 CSVSQFQCSKT-----KCIIKSKRCNGVQECDNGADE--EDCPRSKLCDPDEFRC-GTGLCIKQSQVCDGKMQCLDGLDEEHC---NEEEKCLQGRQFRCANGKSTCLDLIFRCDGVADCEDSSDESDEFCKG-NSSSTCGKMNQFMCADGK----CLRSFQLCDGFPDCLTGEDETECPPSM-CDSTTHLSCLNGQKCISKQLECDGVDDCGDNSDEKHCTEIRLDEAALR 1227 HSP 4 Score: 52.7582 bits (125), Expect = 2.093e-6 Identity = 34/97 (35.05%), Postives = 43/97 (44.33%), Query Frame = 2
Query: 1397 SDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCS-------GLEDENPEFC-KTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADE 1663
SD P C +N+F C + KCL +CD DC L DE PE C + C NE+ C C+ CDG+R CSD +DE
Sbjct: 71 SDEPSTCKRTCLENEFVCKT-----GKCLPRGYLCDSQYDCGRLPNGDRDLSDEAPELCNQPLHCDYNEYACSKSAQCVPLFKFCDGKRDCSDGSDE 162 HSP 5 Score: 50.8322 bits (120), Expect = 9.761e-6 Identity = 60/202 (29.70%), Postives = 80/202 (39.60%), Query Frame = 2
Query: 1469 NRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQ---LQSEIPDSLKHINYPGDHYSL--KTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNC 2059
NRKC + CD + DC DE + C C KS ICDG CS DE NC E+ + K V D L+E + C + C + Y C KQ + + + D ++ D + K C GKY+C++G CI +S CDGR+DCGD SDE C
Sbjct: 3739 NRKCGGFA--CDGIVDCEDGSDE--KGCPDLECH----------LASNKSLICDGTIDCSSGKDENNCHNNGESNFF-QCSMQTIKEWQVCDGRWDCADGLDESPEMCASRKAR--CFRIGY-----------CDDKKQCLDVSTALCDGIRDCFDGSDENPIHCKDMCKGKYRCTNGRCIDVSARCDGRDDCGDGSDEDTC 3912
BLAST of Neurotrypsin vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176]) HSP 1 Score: 71.633 bits (174), Expect = 3.340e-13 Identity = 65/231 (28.14%), Postives = 111/231 (48.05%), Query Frame = 2
Query: 3035 CGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPK--PGMVPDLKTKCLVSGWGDTLVE---------TAEKLLAAEVPLIKAERCKEFYK------DRITDRMQCAGYEAGGIDTCQGDSGGPLVC-QVNGIYTIFGATSWG----DGCA-VPRSPGVYAKVDSMLDFVKEKTG 3658
CG +++ + W+++A+HC + + R FV + NN E+SF ++E +++ Y T D D AL++I + ++PVCL ++ K ++ G+G TL E ++ L + VP+I + C + ++ +IT CAG G T GDSGGPL+ + NG Y G TS+G DG + PGVY ++ + +++ G
Sbjct: 16 CGATVIDDFWLVTAAHCALQLQTRSFVYVREPKNNR----ERSFSVKEAYIHSGYNN-QTADNDIALLRISSDLSKLG-----IKPVCLVHDDSKLLKQYKNGVVI-GYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYLHG--TAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWIQGVIG 233
BLAST of Neurotrypsin vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176]) HSP 1 Score: 71.2478 bits (173), Expect = 8.688e-13 Identity = 65/231 (28.14%), Postives = 111/231 (48.05%), Query Frame = 2
Query: 3035 CGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPK--PGMVPDLKTKCLVSGWGDTLVE---------TAEKLLAAEVPLIKAERCKEFYK------DRITDRMQCAGYEAGGIDTCQGDSGGPLVC-QVNGIYTIFGATSWG----DGCA-VPRSPGVYAKVDSMLDFVKEKTG 3658
CG +++ + W+++A+HC + + R FV + NN E+SF ++E +++ Y T D D AL++I + ++PVCL ++ K ++ G+G TL E ++ L + VP+I + C + ++ +IT CAG G T GDSGGPL+ + NG Y G TS+G DG + PGVY ++ + +++ G
Sbjct: 66 CGATVIDDFWLVTAAHCALQLQTRSFVYVREPKNNR----ERSFSVKEAYIHSGYNN-QTADNDIALLRISSDLSKLG-----IKPVCLVHDDSKLLKQYKNGVVI-GYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYLHG--TAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWIQGVIG 283
BLAST of Neurotrypsin vs. Ensembl Fly
Match: teq (gene:FBgn0023479 transcript:FBtr0076525) HSP 1 Score: 344.354 bits (882), Expect = 2.756e-98 Identity = 212/610 (34.75%), Postives = 306/610 (50.16%), Query Frame = 2
Query: 1943 SLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVD-RACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSV--KLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC--GLPILEHSITTTA----------TSLKKSEQVPGL------------TCG------RKPITQRRGRVVGGFETVYGAFPWQVGVRHR-VGDYDIHWCGGSILSEDWIISASHC-YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT---LVETAEKLLAAEVPLIKAERCKE--FYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
S T C + C +G C+ + CDG+ +C + DE NCP + E+RL+G ++ G++E++ G+WGY+CDD+F + +A V+C++LGF + V GS +D L+DE++C G E + +C W HNC +E+AGV CKV C N + C K CI + +CD DC+D+SDE C V +L G +S GR+E++ G+VCDD F A V C +G G A+ + FG+ G I L C GNETS+ QCN + WGEH C+H ED + C G P + T T+ K Q+ GL CG R RVV G G PWQ +R R G HWCG ++S+ +++A+HC Y PK YFVR GD N + E IE +L+ ++ + D AL + F+D+VQP+CLP KC +SGWG + A+ L +AE+P++ CK+ Y +++ M CAG +D C+GDSGGPLVC + T++G SWG C PGVY +V+ +D++ EK
Sbjct: 844 SFNTQC--DFDCGNGQCLKKEEICDGKKNCPNGKDEANCPPSDY---EVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMKCPNNYWLCHTSKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGVWGSVCDDDFNLKSAQVACNSMGFFGPAK-IEKNIFGNSNGPIWLDQVMCFGNETSIDQCNHWNWGEHNCNHTEDVALHCSAGPPPRSQRYSQTQIKGGRSLGRETTPKTYSQI-GLWERSSKAVHTPRRCGIFKDDLTDEYAHREERVVRGNVAQRGRHPWQATIRTRGRGGISSHWCGAVVISKRHLLTAAHCLYGSPKGAYFVRVGDHYANIAESSEVDSFIENWYLHENFRKGTHMNNDIAL----VVLKTPLKFSDYVQPICLPDKNAELVEDRKCTISGWGSIKSGVSTPAQVLGSAELPILADHVCKQSNVYGSAMSEGMFCAGSMDESVDACEGDSGGPLVCSDDDGETLYGLISWGQHCGFKNRPGVYVRVNHYIDWIYEK 1442 HSP 2 Score: 89.7373 bits (221), Expect = 1.403e-17 Identity = 57/170 (33.53%), Postives = 83/170 (48.82%), Query Frame = 2
Query: 2360 NCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLS---VKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGE---IVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILE 2851
N +EE+ + T +F C + C+K ++CDGK +C + DE C S V+L G GR+E++ + G VCDD F +A V+CR LG AQ V + F + + ++ C GNET L QC WG H C E AGV C +P+++
Sbjct: 829 NSNAEEVTPKLATRSSFNTQCDFDCGNGQ-CLKKEEICDGKKNCPNGKDE--ANCPPSDYEVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMK 995 HSP 3 Score: 60.077 bits (144), Expect = 1.469e-8 Identity = 32/97 (32.99%), Postives = 51/97 (52.58%), Query Frame = 2
Query: 260 DGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
D A P +R G + N GR+EV +WG VCDD + L DA+V C++LGF A+E + +Y++D+++C G+E + +C
Sbjct: 876 DEANCPPSDYEVRLSGGESPNMGRIEV--KANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPN-QDFNYLMDEVECHGNETKLGQC 969 HSP 4 Score: 54.6842 bits (130), Expect = 6.474e-7 Identity = 37/120 (30.83%), Postives = 56/120 (46.67%), Query Frame = 2
Query: 227 SKTIYPIRSVLDGAPETPER-----------LRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGK-WTKKDYIIDKIKCLGSEASIFEC 550
SK P V D P+ ++ ++ R +G +NEGR+EV + WG VCDD +NL A V+C +GF A+ + G W +D++ C G+E SI +C
Sbjct: 1006 SKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGV--WGSVCDDDFNLKSAQVACNSMGFFGPAKIEKNIFGNSNGPIW------LDQVMCFGNETSIDQC 1117
BLAST of Neurotrypsin vs. Ensembl Fly
Match: teq (gene:FBgn0023479 transcript:FBtr0344980) HSP 1 Score: 345.895 bits (886), Expect = 7.501e-98 Identity = 212/610 (34.75%), Postives = 306/610 (50.16%), Query Frame = 2
Query: 1943 SLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVD-RACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSV--KLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC--GLPILEHSITTTA----------TSLKKSEQVPGL------------TCG------RKPITQRRGRVVGGFETVYGAFPWQVGVRHR-VGDYDIHWCGGSILSEDWIISASHC-YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT---LVETAEKLLAAEVPLIKAERCKE--FYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
S T C + C +G C+ + CDG+ +C + DE NCP + E+RL+G ++ G++E++ G+WGY+CDD+F + +A V+C++LGF + V GS +D L+DE++C G E + +C W HNC +E+AGV CKV C N + C K CI + +CD DC+D+SDE C V +L G +S GR+E++ G+VCDD F A V C +G G A+ + FG+ G I L C GNETS+ QCN + WGEH C+H ED + C G P + T T+ K Q+ GL CG R RVV G G PWQ +R R G HWCG ++S+ +++A+HC Y PK YFVR GD N + E IE +L+ ++ + D AL + F+D+VQP+CLP KC +SGWG + A+ L +AE+P++ CK+ Y +++ M CAG +D C+GDSGGPLVC + T++G SWG C PGVY +V+ +D++ EK
Sbjct: 1773 SFNTQC--DFDCGNGQCLKKEEICDGKKNCPNGKDEANCPPSDY---EVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMKCPNNYWLCHTSKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGVWGSVCDDDFNLKSAQVACNSMGFFGPAK-IEKNIFGNSNGPIWLDQVMCFGNETSIDQCNHWNWGEHNCNHTEDVALHCSAGPPPRSQRYSQTQIKGGRSLGRETTPKTYSQI-GLWERSSKAVHTPRRCGIFKDDLTDEYAHREERVVRGNVAQRGRHPWQATIRTRGRGGISSHWCGAVVISKRHLLTAAHCLYGSPKGAYFVRVGDHYANIAESSEVDSFIENWYLHENFRKGTHMNNDIAL----VVLKTPLKFSDYVQPICLPDKNAELVEDRKCTISGWGSIKSGVSTPAQVLGSAELPILADHVCKQSNVYGSAMSEGMFCAGSMDESVDACEGDSGGPLVCSDDDGETLYGLISWGQHCGFKNRPGVYVRVNHYIDWIYEK 2371 HSP 2 Score: 89.3521 bits (220), Expect = 1.959e-17 Identity = 57/170 (33.53%), Postives = 83/170 (48.82%), Query Frame = 2
Query: 2360 NCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLS---VKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGE---IVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILE 2851
N +EE+ + T +F C + C+K ++CDGK +C + DE C S V+L G GR+E++ + G VCDD F +A V+CR LG AQ V + F + + ++ C GNET L QC WG H C E AGV C +P+++
Sbjct: 1758 NSNAEEVTPKLATRSSFNTQCDFDCGNGQ-CLKKEEICDGKKNCPNGKDE--ANCPPSDYEVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMK 1924 HSP 3 Score: 60.4622 bits (145), Expect = 1.360e-8 Identity = 30/91 (32.97%), Postives = 49/91 (53.85%), Query Frame = 2
Query: 278 PERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P +R G + N GR+EV +WG VCDD + L DA+V C++LGF A+E + +Y++D+++C G+E + +C
Sbjct: 1811 PSDYEVRLSGGESPNMGRIEV--KANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPN-QDFNYLMDEVECHGNETKLGQC 1898 HSP 4 Score: 53.1434 bits (126), Expect = 2.368e-6 Identity = 37/120 (30.83%), Postives = 56/120 (46.67%), Query Frame = 2
Query: 227 SKTIYPIRSVLDGAPETPER-----------LRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGK-WTKKDYIIDKIKCLGSEASIFEC 550
SK P V D P+ ++ ++ R +G +NEGR+EV + WG VCDD +NL A V+C +GF A+ + G W +D++ C G+E SI +C
Sbjct: 1935 SKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGV--WGSVCDDDFNLKSAQVACNSMGFFGPAKIEKNIFGNSNGPIW------LDQVMCFGNETSIDQC 2046
BLAST of Neurotrypsin vs. Ensembl Fly
Match: teq (gene:FBgn0023479 transcript:FBtr0344981) HSP 1 Score: 345.51 bits (885), Expect = 1.150e-97 Identity = 212/610 (34.75%), Postives = 306/610 (50.16%), Query Frame = 2
Query: 1943 SLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVD-RACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSV--KLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC--GLPILEHSITTTA----------TSLKKSEQVPGL------------TCG------RKPITQRRGRVVGGFETVYGAFPWQVGVRHR-VGDYDIHWCGGSILSEDWIISASHC-YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT---LVETAEKLLAAEVPLIKAERCKE--FYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
S T C + C +G C+ + CDG+ +C + DE NCP + E+RL+G ++ G++E++ G+WGY+CDD+F + +A V+C++LGF + V GS +D L+DE++C G E + +C W HNC +E+AGV CKV C N + C K CI + +CD DC+D+SDE C V +L G +S GR+E++ G+VCDD F A V C +G G A+ + FG+ G I L C GNETS+ QCN + WGEH C+H ED + C G P + T T+ K Q+ GL CG R RVV G G PWQ +R R G HWCG ++S+ +++A+HC Y PK YFVR GD N + E IE +L+ ++ + D AL + F+D+VQP+CLP KC +SGWG + A+ L +AE+P++ CK+ Y +++ M CAG +D C+GDSGGPLVC + T++G SWG C PGVY +V+ +D++ EK
Sbjct: 2186 SFNTQC--DFDCGNGQCLKKEEICDGKKNCPNGKDEANCPPSDY---EVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMKCPNNYWLCHTSKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGVWGSVCDDDFNLKSAQVACNSMGFFGPAK-IEKNIFGNSNGPIWLDQVMCFGNETSIDQCNHWNWGEHNCNHTEDVALHCSAGPPPRSQRYSQTQIKGGRSLGRETTPKTYSQI-GLWERSSKAVHTPRRCGIFKDDLTDEYAHREERVVRGNVAQRGRHPWQATIRTRGRGGISSHWCGAVVISKRHLLTAAHCLYGSPKGAYFVRVGDHYANIAESSEVDSFIENWYLHENFRKGTHMNNDIAL----VVLKTPLKFSDYVQPICLPDKNAELVEDRKCTISGWGSIKSGVSTPAQVLGSAELPILADHVCKQSNVYGSAMSEGMFCAGSMDESVDACEGDSGGPLVCSDDDGETLYGLISWGQHCGFKNRPGVYVRVNHYIDWIYEK 2784 HSP 2 Score: 89.7373 bits (221), Expect = 1.462e-17 Identity = 57/170 (33.53%), Postives = 83/170 (48.82%), Query Frame = 2
Query: 2360 NCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLS---VKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGE---IVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILE 2851
N +EE+ + T +F C + C+K ++CDGK +C + DE C S V+L G GR+E++ + G VCDD F +A V+CR LG AQ V + F + + ++ C GNET L QC WG H C E AGV C +P+++
Sbjct: 2171 NSNAEEVTPKLATRSSFNTQCDFDCGNGQ-CLKKEEICDGKKNCPNGKDE--ANCPPSDYEVRLSGGESPNMGRIEVKANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPNQDFNYLMDEVECHGNETKLGQCAFKGWGVHNCGVDEVAGVTCKVPVMK 2337 HSP 3 Score: 60.077 bits (144), Expect = 1.624e-8 Identity = 32/97 (32.99%), Postives = 51/97 (52.58%), Query Frame = 2
Query: 260 DGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
D A P +R G + N GR+EV +WG VCDD + L DA+V C++LGF A+E + +Y++D+++C G+E + +C
Sbjct: 2218 DEANCPPSDYEVRLSGGESPNMGRIEV--KANGQWGYVCDDKFGLKDADVVCRELGFQMGAQEVRGSSFYAPPN-QDFNYLMDEVECHGNETKLGQC 2311 HSP 4 Score: 54.6842 bits (130), Expect = 7.197e-7 Identity = 37/120 (30.83%), Postives = 56/120 (46.67%), Query Frame = 2
Query: 227 SKTIYPIRSVLDGAPETPER-----------LRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGK-WTKKDYIIDKIKCLGSEASIFEC 550
SK P V D P+ ++ ++ R +G +NEGR+EV + WG VCDD +NL A V+C +GF A+ + G W +D++ C G+E SI +C
Sbjct: 2348 SKECIPPAFVCDNTPDCADKSDECAAVCQAPVQYRLEGGRNSNEGRLEVKHHGV--WGSVCDDDFNLKSAQVACNSMGFFGPAKIEKNIFGNSNGPIW------LDQVMCFGNETSIDQC 2459
BLAST of Neurotrypsin vs. Ensembl Fly
Match: CG31954 (gene:FBgn0051954 transcript:FBtr0077563) HSP 1 Score: 142.895 bits (359), Expect = 2.015e-37 Identity = 92/244 (37.70%), Postives = 133/244 (54.51%), Query Frame = 2
Query: 2942 GRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHC-YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDT--LVETAEKLLAAEVPLIKAERCKEFYKDR--ITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTG 3658
GR+VGG P QV ++ H CGGSI+SE+WI++A+HC Y K +R VR G +E Q ++++ + ++ D+D +L+++ F++ + V LP+ M C VSGWG+T L+E+ E L EVPL+ E C E YK +T+RM CAG+ GG D CQGDSGGP+V + + G SWG GCA P PGVY++V D++KE +G
Sbjct: 49 GRIVGGHRINITDAPHQVSLQ-----TSSHICGGSIISEEWILTAAHCTYGKTADRLKVRLG---TSEFARSGQLLRVQKIVQHAQFN-YTNVDYDFSLLQLAHPIK----FDETKKAVKLPESQMKYMDGEACFVSGWGNTQNLLESREWLRQVEVPLVNQELCSEKYKQYGGVTERMICAGFLEGGKDACQGDSGGPMVSESG---ELVGVVSWGYGCAKPDYPGVYSRVSFARDWIKEHSG 276
BLAST of Neurotrypsin vs. Ensembl Fly
Match: lambdaTry (gene:FBgn0043470 transcript:FBtr0088121) HSP 1 Score: 135.576 bits (340), Expect = 5.434e-35 Identity = 92/246 (37.40%), Postives = 126/246 (51.22%), Query Frame = 2
Query: 2927 ITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEK---PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLV--ETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
I + GR+VGG +T +P Q+ +R+R H CGG+I + IISA+HC P+N V G N +Q E+ E+ ++PKY+ D+D A++ + + FND VQP+ L K PD T V+GWG T ++ L V ++ CK Y +T RM CAG GG D CQGDSGGPLV T+ G SWG GCA + PGVY V +LD++ E
Sbjct: 29 IPKLDGRIVGGQDTNITQYPHQISMRYRGN----HRCGGTIYRSNQIISAAHCVNTLSGPENLTIV-AGSSNIWFPTGPQQELEVREIIIHPKYR-TLNNDYDAAILILDGDFE----FNDAVQPIELAKER--PDHDTPVTVTGWGTTSEGGTISDVLQEVSVNVVDNSNCKNAYSIMLTSRMLCAGVNGGGKDACQGDSGGPLVYN----NTLLGIVSWGTGCAREKYPGVYCSVPDVLDWLVE 258
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Match: si:ch211-51a6.2 (si:ch211-51a6.2 [Source:ZFIN;Acc:ZDB-GENE-090313-118]) HSP 1 Score: 301.597 bits (771), Expect = 1.317e-89 Identity = 204/567 (35.98%), Postives = 297/567 (52.38%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALE------YVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFE-TVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEK---PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLP----KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGG--IDTCQGDSGGPLVCQVN-GIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
GR++C D M V+ PF +RL G+ + WEG++E+ + GEWG +CDD +T A V+C+QLG+ E Y G+G +ILLDE+ C+G E S+ +C H W +H+C E G+ C +R N E I G + V+LV+G GRVE+ + G+VCDDG+ D A V+CR LG G ++A + FG G G I L N +C G E SL +C++ H C H EDAGVIC + S K E CG +P TQRR R + G + ++ G +PWQV + R H CG ++++ W+I+A+HC+++ +RY +R GD++ E D +E+S E + ++ KY +++D AL+K+K + + FN H VCLP K G P C+++GWG T E + LL A VPL+ + +CK+ Y R T RM CAG + +D+CQGDSGGPLVCQ + G + + G SWG GC P PGVY +V L ++++ T
Sbjct: 42 GRHNC----DHMEDAGVSCNPFTDGVLRLVGADSPWEGRLEVYHAGEWGTVCDDNWTGRHAQVVCRQLGYRGRAEVSPVGMYGEGTG---------MILLDEVTCEGGESSLLDCDHSQWGQHDCSHSEDVGIRC--EREGQNNE---------IPEVAPVTGPL----------------VRLVDGESRKEGRVEVFINAQWGSVCDDGWNDINAQVVCRQLGFVGVSKARSMAYFGEGQGSIHLDNVKCVGTEVSLGECSAVGSDAHDCRHSEDAGVICDYV----TEPVKMNSFGKHE------CGLRPNTQRRRRRIIGGDKSLRGDWPWQVSLWLRSQSKGSHPLCGATLINSCWVITAAHCFKRFGSDASRYVLRLGDYHTEERDDFERSLSPERIVIHKKYH-SQGWEYDIALLKLKGTDENCVAFNPHTNAVCLPPQSSKKGKRP---IACVITGWGITDSEYSRTLLQAWVPLLPSWKCKKRYGSRFTSRMLCAGSLSNHRRVDSCQGDSGGPLVCQGDGGHWMLTGVISWGHGCGDPSYPGVYTRVGRFLKWIEKVT 554 HSP 2 Score: 118.627 bits (296), Expect = 4.877e-27 Identity = 71/207 (34.30%), Postives = 95/207 (45.89%), Query Frame = 2
Query: 2213 GFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
G +A + H FG+ PI L D + C G E S+EECPH W +HNC E AGV C + DG ++LV + GR+E+ + GTVCDD +T A V+CR LG GRA+ +G G G I+L C G E+SL+ C+ +WG+H CSH ED G+ C
Sbjct: 2 GVPKAWTWAH-----FGQGTGPIAL-DGVHCTGNELSLEECPHAPWGRHNCDHMEDAGVSC----------------------NPFTDG-----------------VLRLVGADSPWEGRLEVYHAGEWGTVCDDNWTGRHAQVVCRQLGYRGRAEVSPVGMYGEGTGMILLDEVTCEGGESSLLDCDHSQWGQHDCSHSEDVGIRC 163 HSP 3 Score: 62.3882 bits (150), Expect = 2.834e-9 Identity = 40/95 (42.11%), Postives = 57/95 (60.00%), Query Frame = 2
Query: 266 APETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
AP T +R+ DG+S EGRVEV+ +WG VCDD WN +A V C+QLGFV ++ A + G G+ + +D +KC+G+E S+ EC
Sbjct: 176 APVTGPLVRL-VDGESR-KEGRVEVF--INAQWGSVCDDGWNDINAQVVCRQLGFVGVSK-ARSMAYFGEGQGSIH---LDNVKCVGTEVSLGEC 262 HSP 4 Score: 54.6842 bits (130), Expect = 6.490e-7 Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EGR+EVY++ +WG VCDD W A V C+QLG+ A E + +G G ++D++ C G E+S+ +C
Sbjct: 64 LRLVGADSPWEGRLEVYHAG--EWGTVCDDNWTGRHAQVVCRQLGYRGRA-EVSPVGMYGEGTGM---ILLDEVTCEGGESSLLDC 143
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Match: prss12 (serine protease 12 [Source:ZFIN;Acc:ZDB-GENE-090313-45]) HSP 1 Score: 304.294 bits (778), Expect = 2.609e-89 Identity = 186/565 (32.92%), Postives = 296/565 (52.39%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHW-CGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXX------XXFNDHVQPVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQV-NGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTG 3658
K+ G ++C D++++ V +RL G +EG++E+ Y+G+WG +CDD +T + V+C+QLGF G +FG P+ LLD+++C G E ++ C W KH+C + ++C S C R P L V+LV G + GRVEI GTVCDDG+TD +A V+CR LG SG A+A FG G G I + N +C G E SL C +G H C H EDAGVIC + + S ++ G CG + R+ R++GG ++ G +PWQ VR R D CG +++S WI++++HC+++ N Y VR GD+++ + YE+ + +E++ L+P+Y + D+D AL+++ + + + V P CLP + VP C ++GWGDT ++ L A + ++ +C++ Y ++ T+RM CAG ++A +D+C+GDSGGPLVC+ +G + ++G TSWG C + +PGVY KV + + ++K+ G
Sbjct: 148 KTAWGEHNC-DHTEDAGVSCVPLTDGAVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFRSGESVSAG---RFGIAAGPV-LLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVC----------------------SPQCS-------RHGVPNS---LPVRLVGGENEREGRVEILVGGQWGTVCDDGWTDRDAEVVCRQLGYSGLAKARVMAYFGEGEGPIHIDNVKCTGGEHSLTDCIKQPFGTHNCRHSEDAGVICNYGLQQDS--------PGVKEAVGSMCGLRLSHTRQKRIIGGENSLRGGWPWQAAVRLRGSQADGRLVCGATLISSCWILTSAHCFKRYGNSTGLYKVRVGDYHSLVPEEYEEEYAVEKIVLHPRYHADSS-DYDLALLRVSGHRAHRGVTGQCVTLSRSVLPACLPVRREKVPKGIANCYITGWGDTGRAYSKTLQQASISVLSKRQCEQRYSEQFTNRMLCAGSSHDAQRVDSCRGDSGGPLVCERPSGSWVVYGVTSWGHACRLQEAPGVYTKVSAFVPWIKKVIG 666 HSP 2 Score: 152.91 bits (385), Expect = 1.098e-37 Identity = 86/270 (31.85%), Postives = 135/270 (50.00%), Query Frame = 2
Query: 2051 MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
M + F P +RL+G + +EG+VE+ + G+WG +CDD++ ++A V+C+QLG + G+ G R + LDE+ C G E SIE+CP W +HNC E AGV +C+ ++ DG +V+LV G Y GR+E+ GTVCDDG+T + V+CR LG ++V+ RFG G ++L + RC G E ++ C+ W +H C+H++D ++C H +
Sbjct: 57 MPVETSVFVP--VRLSGGPSAYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGIAKAWVGAYFGAGSGR---VFLDEVSCTGNELSIEQCPKTAWGEHNCDHTEDAGV-------------------SCVPLT---DG-----------------AVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFR-SGESVSAGRFGIAAGPVLLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVCSPQCSRHGV 281 HSP 3 Score: 60.077 bits (144), Expect = 1.696e-8 Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET + +R G +A EGRVEVY++ +WG VCDD W+ DA V C+QLG A+ A + G G +D++ C G+E SI +C
Sbjct: 60 ETSVFVPVRLSGGPSAYEGRVEVYHAG--QWGTVCDDQWDDNDAEVVCRQLGLGGIAK-AWVGAYFGAG---SGRVFLDEVSCTGNELSIEQC 146 HSP 4 Score: 56.9954 bits (136), Expect = 1.229e-7 Identity = 35/92 (38.04%), Postives = 46/92 (50.00%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P L +R G EGRVE+ +WG VCDD W DA V C+QLG+ A+ A + G G + ID +KC G E S+ +C
Sbjct: 281 VPNSLPVRLVGGENEREGRVEILVGG--QWGTVCDDGWTDRDAEVVCRQLGYSGLAK-ARVMAYFGEG---EGPIHIDNVKCTGGEHSLTDC 366
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Match: BX511095.2 (serine protease 12 [Source:NCBI gene;Acc:566809]) HSP 1 Score: 303.523 bits (776), Expect = 1.186e-87 Identity = 186/565 (32.92%), Postives = 295/565 (52.21%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHW-CGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXX------XXFNDHVQPVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTG 3658
K+ G ++C D++++ V +RL G +EG++E+ Y+G+WG +CDD +T + V+C+QLGF G +FG P+ LLD+++C G E ++ C W KH+C + ++C S C R P L V+LV G + GRVEI GTVCDDG+TD +A V+CR LG SG A+A FG G G I + N +C G E SL C +G H C H EDAGVIC + + S S+ CG + R+ R++GG ++ G +PWQ VR R D CG +++S WI++++HC+++ N Y VR GD+++ + YE+ + +E++ L+P+Y + D+D AL+++ + + + V P CLP + VP C ++GWGDT ++ L A + ++ +C++ Y ++ T+RM CAG ++A +D+C+GDSGGPLVC+ +G + ++G TSWG C + +PGVY KV + + ++K+ G
Sbjct: 299 KTAWGEHNC-DHTEDAGVSCVPLTDGAVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFRSGESVSAG---RFGIAAGPV-LLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVC----------------------SPQCS-------RHGVPNS---LPVRLVGGENEREGRVEILVGGQWGTVCDDGWTDRDAEVVCRQLGYSGLAKARVMAYFGEGEGPIHIDNVKCTGGEHSLTDCIKQPFGTHNCRHSEDAGVICNYGLQQDSPGVKVGSM----------CGLRLSHTRQKRIIGGENSLRGGWPWQAAVRLRGSQADGRLVCGATLISSCWILTSAHCFKRYGNSTGLYKVRVGDYHSLVPEEYEEEYAVEKIVLHPRYHADSS-DYDLALLRVSGHRAHRGVTGQCVTLSRSVLPACLPVRREKVPKGIANCYITGWGDTGRAYSKTLQQASISVLSKRQCEQRYSEQFTNRMLCAGSSHDAQRVDSCRGDSGGPLVCERPSGSWVVYGVTSWGHACRLQEAPGVYTKVSAFVPWIKKVIG 815 HSP 2 Score: 153.295 bits (386), Expect = 1.975e-37 Identity = 86/270 (31.85%), Postives = 135/270 (50.00%), Query Frame = 2
Query: 2051 MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
M + F P +RL+G + +EG+VE+ + G+WG +CDD++ ++A V+C+QLG + G+ G R + LDE+ C G E SIE+CP W +HNC E AGV +C+ ++ DG +V+LV G Y GR+E+ GTVCDDG+T + V+CR LG ++V+ RFG G ++L + RC G E ++ C+ W +H C+H++D ++C H +
Sbjct: 208 MPVETSVFVP--VRLSGGPSAYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGIAKAWVGAYFGAGSGR---VFLDEVSCTGNELSIEQCPKTAWGEHNCDHTEDAGV-------------------SCVPLT---DG-----------------AVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFR-SGESVSAGRFGIAAGPVLLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVCSPQCSRHGV 432 HSP 3 Score: 137.502 bits (345), Expect = 1.927e-32 Identity = 80/258 (31.01%), Postives = 120/258 (46.51%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
+RL G ++ +G VE+ G WG IC + + +A V+C+QLG H ++ ++ + C+G E + +C VW C++ + A V C E F+ V+L GP +Y GRVE+ + GTVCDD + D++A V+CR LG+ G A+A FG+G G + L C GNE S+ QC WGEH C H EDAGV C +P+ + ++
Sbjct: 108 LRLQGGRSKLDGTVEVYLNGVWGTICSNGWDDQDAAVVCRQLG---QGYRAHARMSPLSRLVVSVVHWQTVGCEGQEDDLLQCNRTVWNGGECENRQAAAVSCM------------------------------------PVETSVFVPVRLSGGPSAYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGIAKAWVGAYFGAGSGRVFLDEVSCTGNELSIEQCPKTAWGEHNCDHTEDAGVSC-VPLTDGAV 325 HSP 4 Score: 60.077 bits (144), Expect = 1.763e-8 Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET + +R G +A EGRVEVY++ +WG VCDD W+ DA V C+QLG A+ A + G G +D++ C G+E SI +C
Sbjct: 211 ETSVFVPVRLSGGPSAYEGRVEVYHAG--QWGTVCDDQWDDNDAEVVCRQLGLGGIAK-AWVGAYFGAG---SGRVFLDEVSCTGNELSIEQC 297 HSP 5 Score: 58.151 bits (139), Expect = 5.798e-8 Identity = 34/105 (32.38%), Postives = 52/105 (49.52%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPI 2845
S++L G G VE+ + GT+C +G+ D +A+V+CR LG RA A V + C G E L+QCN W +C +++ A V C +P+
Sbjct: 107 SLRLQGGRSKLDGTVEVYLNGVWGTICSNGWDDQDAAVVCRQLGQGYRAHARMSPLSRLVVSVVHWQTVGCEGQEDDLLQCNRTVWNGGECENRQAAAVSC-MPV 210 HSP 6 Score: 57.3806 bits (137), Expect = 1.079e-7 Identity = 31/78 (39.74%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 1118 TDCYE--GNGATYRGTTSRTNQGTKCAKWSN-PSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGV--YGYC 1336
TDC + G Y G+ T G KC WS+ P + + + GLGDH +CRNPD I PWC+ + G +GYC
Sbjct: 27 TDCVQSKGKAGFYEGSIDATESGVKCMNWSDVPGVR---ERYQGKGLGDHTFCRNPDGRIRPWCFFTNNRGRIDWGYC 101 HSP 7 Score: 56.225 bits (134), Expect = 2.633e-7 Identity = 35/92 (38.04%), Postives = 46/92 (50.00%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P L +R G EGRVE+ +WG VCDD W DA V C+QLG+ A+ A + G G + ID +KC G E S+ +C
Sbjct: 432 VPNSLPVRLVGGENEREGRVEILVGG--QWGTVCDDGWTDRDAEVVCRQLGYSGLAK-ARVMAYFGEG---EGPIHIDNVKCTGGEHSLTDC 517
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Match: prss12 (serine protease 12 [Source:ZFIN;Acc:ZDB-GENE-090313-45]) HSP 1 Score: 303.523 bits (776), Expect = 1.209e-87 Identity = 186/565 (32.92%), Postives = 295/565 (52.21%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHW-CGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXX------XXFNDHVQPVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTG 3658
K+ G ++C D++++ V +RL G +EG++E+ Y+G+WG +CDD +T + V+C+QLGF G +FG P+ LLD+++C G E ++ C W KH+C + ++C S C R P L V+LV G + GRVEI GTVCDDG+TD +A V+CR LG SG A+A FG G G I + N +C G E SL C +G H C H EDAGVIC + + S S+ CG + R+ R++GG ++ G +PWQ VR R D CG +++S WI++++HC+++ N Y VR GD+++ + YE+ + +E++ L+P+Y + D+D AL+++ + + + V P CLP + VP C ++GWGDT ++ L A + ++ +C++ Y ++ T+RM CAG ++A +D+C+GDSGGPLVC+ +G + ++G TSWG C + +PGVY KV + + ++K+ G
Sbjct: 299 KTAWGEHNC-DHTEDAGVSCVPLTDGAVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFRSGESVSAG---RFGIAAGPV-LLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVC----------------------SPQCS-------RHGVPNS---LPVRLVGGENEREGRVEILVGGQWGTVCDDGWTDRDAEVVCRQLGYSGLAKARVMAYFGEGEGPIHIDNVKCTGGEHSLTDCIKQPFGTHNCRHSEDAGVICNYGLQQDSPGVKVGSM----------CGLRLSHTRQKRIIGGENSLRGGWPWQAAVRLRGSQADGRLVCGATLISSCWILTSAHCFKRYGNSTGLYKVRVGDYHSLVPEEYEEEYAVEKIVLHPRYHADSS-DYDLALLRVSGHRAHRGVTGQCVTLSRSVLPACLPVRREKVPKGIANCYITGWGDTGRAYSKTLQQASISVLSKRQCEQRYSEQFTNRMLCAGSSHDAQRVDSCRGDSGGPLVCERPSGSWVVYGVTSWGHACRLQEAPGVYTKVSAFVPWIKKVIG 815 HSP 2 Score: 153.295 bits (386), Expect = 2.082e-37 Identity = 86/270 (31.85%), Postives = 135/270 (50.00%), Query Frame = 2
Query: 2051 MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
M + F P +RL+G + +EG+VE+ + G+WG +CDD++ ++A V+C+QLG + G+ G R + LDE+ C G E SIE+CP W +HNC E AGV +C+ ++ DG +V+LV G Y GR+E+ GTVCDDG+T + V+CR LG ++V+ RFG G ++L + RC G E ++ C+ W +H C+H++D ++C H +
Sbjct: 208 MPVETSVFVP--VRLSGGPSAYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGIAKAWVGAYFGAGSGR---VFLDEVSCTGNELSIEQCPKTAWGEHNCDHTEDAGV-------------------SCVPLT---DG-----------------AVRLVGGVKGYEGRLEVHYRGQWGTVCDDGWTQTNTQVVCRQLGFR-SGESVSAGRFGIAAGPVLLDDVRCTGKEPTVTHCSRRGWFKHDCTHRQDVAIVCSPQCSRHGV 432 HSP 3 Score: 137.502 bits (345), Expect = 2.084e-32 Identity = 80/258 (31.01%), Postives = 120/258 (46.51%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
+RL G ++ +G VE+ G WG IC + + +A V+C+QLG H ++ ++ + C+G E + +C VW C++ + A V C E F+ V+L GP +Y GRVE+ + GTVCDD + D++A V+CR LG+ G A+A FG+G G + L C GNE S+ QC WGEH C H EDAGV C +P+ + ++
Sbjct: 108 LRLQGGRSKLDGTVEVYLNGVWGTICSNGWDDQDAAVVCRQLG---QGYRAHARMSPLSRLVVSVVHWQTVGCEGQEDDLLQCNRTVWNGGECENRQAAAVSCM------------------------------------PVETSVFVPVRLSGGPSAYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGIAKAWVGAYFGAGSGRVFLDEVSCTGNELSIEQCPKTAWGEHNCDHTEDAGVSC-VPLTDGAV 325 HSP 4 Score: 60.077 bits (144), Expect = 1.793e-8 Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET + +R G +A EGRVEVY++ +WG VCDD W+ DA V C+QLG A+ A + G G +D++ C G+E SI +C
Sbjct: 211 ETSVFVPVRLSGGPSAYEGRVEVYHAG--QWGTVCDDQWDDNDAEVVCRQLGLGGIAK-AWVGAYFGAG---SGRVFLDEVSCTGNELSIEQC 297 HSP 5 Score: 58.151 bits (139), Expect = 6.050e-8 Identity = 34/105 (32.38%), Postives = 52/105 (49.52%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPI 2845
S++L G G VE+ + GT+C +G+ D +A+V+CR LG RA A V + C G E L+QCN W +C +++ A V C +P+
Sbjct: 107 SLRLQGGRSKLDGTVEVYLNGVWGTICSNGWDDQDAAVVCRQLGQGYRAHARMSPLSRLVVSVVHWQTVGCEGQEDDLLQCNRTVWNGGECENRQAAAVSC-MPV 210 HSP 6 Score: 57.3806 bits (137), Expect = 1.034e-7 Identity = 31/78 (39.74%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 1118 TDCYE--GNGATYRGTTSRTNQGTKCAKWSN-PSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGV--YGYC 1336
TDC + G Y G+ T G KC WS+ P + + + GLGDH +CRNPD I PWC+ + G +GYC
Sbjct: 27 TDCVQSKGKAGFYEGSIDSTESGVKCMNWSDVPGVR---ERYQGKGLGDHTFCRNPDGRIRPWCFFTNNRGRIDWGYC 101 HSP 7 Score: 56.225 bits (134), Expect = 2.656e-7 Identity = 35/92 (38.04%), Postives = 46/92 (50.00%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P L +R G EGRVE+ +WG VCDD W DA V C+QLG+ A+ A + G G + ID +KC G E S+ +C
Sbjct: 432 VPNSLPVRLVGGENEREGRVEILVGG--QWGTVCDDGWTDRDAEVVCRQLGYSGLAK-ARVMAYFGEG---EGPIHIDNVKCTGGEHSLTDC 517
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Match: si:ch73-127m5.1 (si:ch73-127m5.1 [Source:ZFIN;Acc:ZDB-GENE-130531-25]) HSP 1 Score: 301.982 bits (772), Expect = 7.136e-87 Identity = 192/557 (34.47%), Postives = 289/557 (51.89%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGL-PILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGVRHRV---GDYDIHWCGGSILSEDWIISASHCYEK---PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLK-TKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDR-MQCAGYEAGGI----DTCQGDSGGPLVCQVN-GIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
N D M V+ P+ +RL N WEG++E+ + +WG +CDD +T A V+C+QLGF E G +ILLD+++C+GTE S+ EC H VW +H+C E G+ C +V+ ++ PE L V+L G GRVE+ + G+VCD G+ D A+V+CR LG +G ++A FG+G G I LSN RC G E+ L +C S H C H +DAGV+C P E + T TCG + +RR R+VGG++++ G +PWQ + R G+ + CG ++++ W+++A+HC+++ +RY V+ GD++ E D +E+ E + ++ KY +++ D ALI++K G + FN H CLP PG + C+++GWG T E LL A VPL+ + +CK+ Y +R T M CAG + D+CQGDSGGPLVCQ G + + G SWG GC P PGVY++V L ++++ T
Sbjct: 315 NCDHMEDAGVSCNPYTDGVVRLVDGDNPWEGRLEVYHSRDWGTVCDDSWTEPHAQVVCRQLGFRGGAEVAPAGAFGEGT---GLILLDDVECEGTESSLLECKHGVWGRHDCSHSEDVGIRCH---------------------------RVENNEVPLAPESTGPL-VRLAGGDSRKEGRVEVFLNGEWGSVCDHGWNDVNAAVVCRQLGFNGESKARPMGYFGAGKGTIHLSNVRCTGKESLLGECPSKGQDSHVCKHGQDAGVVCDYEPEPEADVAVVGTH----------TCGLRAGAERRSKRIVGGYKSLRGDWPWQASLWLRSQSKGNQPL--CGATLINSCWLLTAAHCFKRFGSDASRYVVKLGDYHTREQDDFERVLSPEHIEVHKKYH-TDSWEHDVALIRLKGTEGKCVAFNPHTNAACLPAPGSKWGKRPASCVITGWGMTDTEHPSTLLQAWVPLLPSWQCKKRYGERFTSHDMLCAGSMTSDLRKHADSCQGDSGGPLVCQGEAGRWVLTGVISWGHGCGDPSYPGVYSRVSRYLGWIEQVT 827 HSP 2 Score: 163.31 bits (412), Expect = 1.658e-40 Identity = 95/261 (36.40%), Postives = 131/261 (50.19%), Query Frame = 2
Query: 2060 PSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
P PP +RL G + D+EG+VE+ + G WG ICDD++ +A V+C+QLG +A + H FG+ P I LD ++C G E S++EC H W +HNC E AGV C + DG V+LV+G + + GR+E+ S GTVCDD +T+ A V+CR LG G A+ FG G G I+L + C G E+SL++C WG H CSH ED G+ C
Sbjct: 220 PEGNGPP--LRLVGGEEDFEGRVEVFHDGRWGTICDDQWDDMDAEVVCRQLGLGGIPKAWSWAH-----FGQGSGP-IQLDAVQCTGNELSLDECRHSGWEQHNCDHMEDAGVSC----------------------NPYTDG-----------------VVRLVDGDNPWEGRLEVYHSRDWGTVCDDSWTEPHAQVVCRQLGFRGGAEVAPAGAFGEGTGLILLDDVECEGTESSLLECKHGVWGRHDCSHSEDVGIRC 433 HSP 3 Score: 124.02 bits (310), Expect = 2.992e-28 Identity = 84/255 (32.94%), Postives = 120/255 (47.06%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIE---ALEYVH---GSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G + G++E+ G+WG +CD +T +A V+C+QLG E AL++ + GSG+ F R L C+G E S+ EC + +C AGV+C + PEG ++LV G + + GRVE+ GT+CDD + D +A V+CR LG+ G +A + FG G G I L +C GNE SL +C W +H C H EDAGV C
Sbjct: 118 VRLVGGSSLKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGLGEIGTALQHSYFGPGSGL-FHYAR--------LGCRGDEKSLLECRSRKFVTSDCNHGSEAGVVC------------------------------------EPPEGN-GPPLRLVGGEEDFEGRVEVFHDGRWGTICDDQWDDMDAEVVCRQLGLGGIPKAWSWAHFGQGSGPIQLDAVQCTGNELSLDECRHSGWEQHNCDHMEDAGVSC 326 HSP 4 Score: 80.8777 bits (198), Expect = 6.672e-15 Identity = 42/104 (40.38%), Postives = 62/104 (59.62%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLP 2842
+V+LV G SGR+E+ + G+VCD +TD +ASV+CR LG+ A+ + FG G G + CRG+E SL++C S K+ C+H +AGV+C P
Sbjct: 117 AVRLVGGSSLKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGLGEIGTALQHSYFGPGSGLFHYARLGCRGDEKSLLECRSRKFVTSDCNHGSEAGVVCEPP 220 HSP 5 Score: 60.4622 bits (145), Expect = 1.245e-8 Identity = 33/76 (43.42%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGR+EVY+S+ WG VCDD W P A V C+QLGF A E G G ++D ++C G+E+S+ EC
Sbjct: 344 EGRLEVYHSR--DWGTVCDDSWTEPHAQVVCRQLGFRGGA-EVAPAGAFGEG---TGLILLDDVECEGTESSLLEC 413 HSP 6 Score: 58.151 bits (139), Expect = 6.105e-8 Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
Y GT S+T+ G+ C KW+ V + +P GLG+HN+CRNPD + PWC+ G G+
Sbjct: 49 YNGTVSQTDSGSPCLKWTEFPDYV--QQYPGRGLGEHNFCRNPDRESNPWCFFRQSSGAIGW 108 HSP 7 Score: 55.4546 bits (132), Expect = 4.348e-7 Identity = 37/95 (38.95%), Postives = 51/95 (53.68%), Query Frame = 2
Query: 266 APETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
APE+ L +R G + EGRVEV+ +WG VCD WN +A V C+QLGF N +A + G GK T + ++C G E+ + EC
Sbjct: 444 APESTGPL-VRLAGGDSRKEGRVEVF--LNGEWGSVCDHGWNDVNAAVVCRQLGF-NGESKARPMGYFGAGKGTIH---LSNVRCTGKESLLGEC 531 HSP 8 Score: 53.5286 bits (127), Expect = 1.781e-6 Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G EGRVEV++ +WG +CDD W+ DA V C+QLG + +A H G+G + +D ++C G+E S+ EC
Sbjct: 227 LRLVGGEEDFEGRVEVFHDG--RWGTICDDQWDDMDAEVVCRQLG-LGGIPKAWSWAHFGQGSGPIQ---LDAVQCTGNELSLDEC 306
BLAST of Neurotrypsin vs. Ensembl Xenopus
Match: ENSXETT00000001485.1 (neurotrypsin-like [Source:NCBI gene;Acc:100497588]) HSP 1 Score: 326.635 bits (836), Expect = 9.199e-96 Identity = 254/878 (28.93%), Postives = 400/878 (45.56%), Query Frame = 2
Query: 1079 LPFICQKITTRGRTDCYEG--NGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELP---RISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRH-------CSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYE---KPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
L C G + C +G + Y GT S T G++C W + + +P GLGDHN+CRNPD ++PWC+ GV + + CS + ++ LV +L + + G G + + S + L +S + + PE + C+ NE KA C ++ H CS + D + ++ ++++ ++ ++ + C + CR L + + +++ Q P L ++ G+ SL K++ +CD D G + D F +RL+G EG VE+ Y G+WG +CDD +T A V+C+QLGF S +FG + I LD++ C G E S+ ECPH W +H+C E G V CS+ S++ G V+L++G + GRVE+ + G+VCDDG+TD +A+V+CR LG G A+A T FG G G I + N C G+E +L +C G H C H EDAGVIC I +K V + CG + R+ R+VGG ++ G++PWQ +R + + CG ++++ W ++A+HC++ K +RY +R GD++ D +E+ ++++ ++ Y+ D D AL++++ G F+ HV P+CLP+ + C +SGWGDT + LL V ++ + C YK + T+RM CAG +E ID+CQGDSGGPL+CQ NG + I G TSWG GC PGVY KV ++K+
Sbjct: 18 LKSCCMLFLLAGYSLCSDGIDSLGYYNGTISVTETGSECLHWKEFPDYI--QQYPGRGLGDHNHCRNPDGGVVPWCFYRKSSGVISWAHCD-CS-QGAVRLVGDLSFNQGLVELYLNGQWGTICGEQWTDWDASVVCRQLGVSEIGTALISTRSGSWLAPEHLQAANCRGNE---KALLHCHYSKSLNHRSSHGLIAAVTCSSSKDSQ-----TPLRLVGGKERYEGRV-----EVFHEGRWGTICDDHWDDSDAEVICRQLGFSGKPKAWVWAH-----YGQGSGPILLDEVHCIGNESSLDLCEKSKWE---------HHNCDHIEDAGVSCD-------PFTDGALRLSGGARPGEGLVEVYYNGDWGSVCDDGWTELNAQVVCRQLGFSGPSSL--SSKAEFGS-GEGFIFLDDVTCTGEEESLLECPHSSWGQHDCSHSEDVG--------------------------------VRCSNESNDIIGP---PVRLMDGESTKEGRVEVFLNGEWGSVCDDGWTDDDAAVVCRQLGHRGPAKARTMAYFGEGQGPIHMDNVECSGSENTLAECRKQDSGIHNCWHNEDAGVICDY------IEKRDNQVKAKGGVSSV-CGISLVPHRKKRIVGGTKSARGSWPWQASLRLKGFHKETRLLCGATLINNCWALTAAHCFKRFGKQVHRYSLRVGDYHTGVEDEFERELPLQKIVIHKNYRSTSN-DNDIALVRVQGKEGHCFPFSLHVLPICLPEKKEKTNNWQPCFISGWGDTGTSYSRTLLQGAVSVLPKDTCILRYKGKFTNRMVCAGTLFEDRLIDSCQGDSGGPLMCQRSNGQWIIVGITSWGYGCGRKGYPGVYTKVSKFTPWIKKAA 811 HSP 2 Score: 59.6918 bits (143), Expect = 3.380e-8 Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+TP RL G EGRVEV++ +WG +CDD+W+ DA V C+QLGF + +A H+G+G ++D++ C+G+E+S+ C
Sbjct: 218 QTPLRLV----GGKERYEGRVEVFHEG--RWGTICDDHWDDSDAEVICRQLGF-SGKPKAWVWAHYGQG---SGPILLDEVHCIGNESSLDLC 300 HSP 3 Score: 51.9878 bits (123), Expect = 7.594e-6 Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EG VEVY + WG VCDD W +A V C+QLGF + +++ + G G + +D + C G E S+ EC
Sbjct: 328 LRLSGGARPGEGLVEVYYNG--DWGSVCDDGWTELNAQVVCRQLGFSGPSSLSSKAE-FGSG---EGFIFLDDVTCTGEEESLLEC 407
BLAST of Neurotrypsin vs. Ensembl Xenopus
Match: ENSXETT00000001560.1 (neurotrypsin-like [Source:NCBI gene;Acc:100497588]) HSP 1 Score: 325.479 bits (833), Expect = 2.131e-95 Identity = 250/853 (29.31%), Postives = 393/853 (46.07%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELP---RISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRH-------CSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYE---KPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
Y GT S T G++C W + + +P GLGDHN+CRNPD ++PWC+ GV + + CS + ++ LV +L + + G G + + S + L +S + + PE + C+ NE KA C ++ H CS + D + ++ ++++ ++ ++ + C + CR L + + +++ Q P L ++ G+ SL K++ +CD D G + D F +RL+G EG VE+ Y G+WG +CDD +T A V+C+QLGF S +FG + I LD++ C G E S+ ECPH W +H+C E GV RC + +D P G V+L++G + GRVE+ + G+VCDDG+TD +A+V+CR LG G A+A T FG G G I + N C G+E +L +C G H C H EDAGVIC I +K V + CG + R+ R+VGG ++ G++PWQ +R + + CG ++++ W ++A+HC++ K +RY +R GD++ D +E+ ++++ ++ Y+ + D D AL++++ G F+ HV P+CLP+ + C +SGWGDT + LL V ++ + C YK + T+RM CAG +E ID+CQGDSGGPL+CQ NG + I G TSWG GC PGVY KV ++K+
Sbjct: 35 YNGTISVTETGSECLHWKEFPDYI--QQYPGRGLGDHNHCRNPDGGVVPWCFYRKSSGVISWAHCD-CS-QGAVRLVGDLSFNQGLVELYLNGQWGTICGEQWTDWDASVVCRQLGVSEIGTALISTRSGSWLAPEHLQAANCRGNE---KALLHCHYSKSLNHRSSHGLIAAVTCSSSKDSQ-----TPLRLVGGKERYEGRV-----EVFHEGRWGTICDDHWDDSDAEVICRQLGFSGKPKAWVWAH-----YGQGSGPILLDEVHCIGNESSLDLCEKSKWE---------HHNCDHIEDAGVSCD-------PFTDGALRLSGGARPGEGLVEVYYNGDWGSVCDDGWTELNAQVVCRQLGFSGPSSL--SSKAEFGS-GEGFIFLDDVTCTGEEESLLECPHSSWGQHDCSHSEDVGV-------------RCSNES---------------NDIIGIPTGP---PVRLMDGESTKEGRVEVFLNGEWGSVCDDGWTDDDAAVVCRQLGHRGPAKARTMAYFGEGQGPIHMDNVECSGSENTLAECRKQDSGIHNCWHNEDAGVICDY------IEKRDNQVKAKGGVSSV-CGISLVPHRKKRIVGGTKSARGSWPWQASLRLKGFHKETRLLCGATLINNCWALTAAHCFKRFGKQVHRYSLRVGDYHTGVEDEFERELPLQKIVIHKNYR-STSNDNDIALVRVQGKEGHCFPFSLHVLPICLPEKKEKTNNWQPCFISGWGDTGTSYSRTLLQGAVSVLPKDTCILRYKGKFTNRMVCAGTLFEDRLIDSCQGDSGGPLMCQRSNGQWIIVGITSWGYGCGRKGYPGVYTKVSKFTPWIKKAA 807 HSP 2 Score: 59.3066 bits (142), Expect = 3.672e-8 Identity = 35/93 (37.63%), Postives = 54/93 (58.06%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+TP RL G EGRVEV++ +WG +CDD+W+ DA V C+QLGF + A H+G+G ++D++ C+G+E+S+ C
Sbjct: 210 QTPLRLV----GGKERYEGRVEVFHEG--RWGTICDDHWDDSDAEVICRQLGFSGKPK-AWVWAHYGQG---SGPILLDEVHCIGNESSLDLC 292 HSP 3 Score: 51.9878 bits (123), Expect = 8.047e-6 Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EG VEVY + WG VCDD W +A V C+QLGF + +++ + G G + +D + C G E S+ EC
Sbjct: 320 LRLSGGARPGEGLVEVYYNG--DWGSVCDDGWTELNAQVVCRQLGFSGPSSLSSKAE-FGSG---EGFIFLDDVTCTGEEESLLEC 399
BLAST of Neurotrypsin vs. Ensembl Xenopus
Match: ENSXETT00000001537.1 (neurotrypsin-like [Source:NCBI gene;Acc:100497588]) HSP 1 Score: 325.479 bits (833), Expect = 3.232e-95 Identity = 250/853 (29.31%), Postives = 392/853 (45.96%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELP---RISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRH-------CSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYE---KPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
Y GT S T G++C W + + +P GLGDHN+CRNPD ++PWC+ GV + + CS + ++ LV +L + + G G + + S + L +S + + PE + C+ NE KA C ++ H CS + D + ++ ++++ ++ ++ + C + CR L + + +++ Q P L ++ G+ SL K++ +CD D G + D F +RL+G EG VE+ Y G+WG +CDD +T A V+C+QLGF S +FG + I LD++ C G E S+ ECPH W +H+C E GV RC + +D P G V+L++G + GRVE+ + G+VCDDG+TD +A+V+CR LG G A+A T FG G G I + N C G+E +L +C G H C H EDAGVIC I +K V + CG + R+ R+VGG ++ G++PWQ +R + + CG ++++ W ++A+HC++ K +RY +R GD++ D +E+ ++++ ++ Y+ D D AL++++ G F+ HV P+CLP+ + C +SGWGDT + LL V ++ + C YK + T+RM CAG +E ID+CQGDSGGPL+CQ NG + I G TSWG GC PGVY KV ++K+
Sbjct: 54 YNGTISVTETGSECLHWKEFPDYI--QQYPGRGLGDHNHCRNPDGGVVPWCFYRKSSGVISWAHCD-CS-QGAVRLVGDLSFNQGLVELYLNGQWGTICGEQWTDWDASVVCRQLGVSEIGTALISTRSGSWLAPEHLQAANCRGNE---KALLHCHYSKSLNHRSSHGLIAAVTCSSSKDSQ-----TPLRLVGGKERYEGRV-----EVFHEGRWGTICDDHWDDSDAEVICRQLGFSGKPKAWVWAH-----YGQGSGPILLDEVHCIGNESSLDLCEKSKWE---------HHNCDHIEDAGVSCD-------PFTDGALRLSGGARPGEGLVEVYYNGDWGSVCDDGWTELNAQVVCRQLGFSGPSSL--SSKAEFGS-GEGFIFLDDVTCTGEEESLLECPHSSWGQHDCSHSEDVGV-------------RCSNES---------------NDIIGIPTGP---PVRLMDGESTKEGRVEVFLNGEWGSVCDDGWTDDDAAVVCRQLGHRGPAKARTMAYFGEGQGPIHMDNVECSGSENTLAECRKQDSGIHNCWHNEDAGVICDY------IEKRDNQVKAKGGVSSV-CGISLVPHRKKRIVGGTKSARGSWPWQASLRLKGFHKETRLLCGATLINNCWALTAAHCFKRFGKQVHRYSLRVGDYHTGVEDEFERELPLQKIVIHKNYRSTSN-DNDIALVRVQGKEGHCFPFSLHVLPICLPEKKEKTNNWQPCFISGWGDTGTSYSRTLLQGAVSVLPKDTCILRYKGKFTNRMVCAGTLFEDRLIDSCQGDSGGPLMCQRSNGQWIIVGITSWGYGCGRKGYPGVYTKVSKFTPWIKKAA 826 HSP 2 Score: 59.6918 bits (143), Expect = 3.376e-8 Identity = 35/93 (37.63%), Postives = 54/93 (58.06%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+TP RL G EGRVEV++ +WG +CDD+W+ DA V C+QLGF + A H+G+G ++D++ C+G+E+S+ C
Sbjct: 229 QTPLRLV----GGKERYEGRVEVFHEG--RWGTICDDHWDDSDAEVICRQLGFSGKPK-AWVWAHYGQG---SGPILLDEVHCIGNESSLDLC 311 HSP 3 Score: 51.9878 bits (123), Expect = 7.640e-6 Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EG VEVY + WG VCDD W +A V C+QLGF + +++ + G G + +D + C G E S+ EC
Sbjct: 339 LRLSGGARPGEGLVEVYYNG--DWGSVCDDGWTELNAQVVCRQLGFSGPSSLSSKAE-FGSG---EGFIFLDDVTCTGEEESLLEC 418 HSP 4 Score: 51.6026 bits (122), Expect = 9.774e-6 Identity = 37/91 (40.66%), Postives = 49/91 (53.85%), Query Frame = 2
Query: 278 PERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P RL DG+S EGRVEV+ +WG VCDD W DA V C+QLG A+ T + G G + +D ++C GSE ++ EC
Sbjct: 453 PVRLM---DGEST-KEGRVEVF--LNGEWGSVCDDGWTDDDAAVVCRQLGHRGPAKARTMA-YFGEG---QGPIHMDNVECSGSENTLAEC 533
BLAST of Neurotrypsin vs. Ensembl Xenopus
Match: tmem132a (transmembrane protein 132A [Source:Xenbase;Acc:XB-GENE-1009879]) HSP 1 Score: 301.597 bits (771), Expect = 1.925e-87 Identity = 191/564 (33.87%), Postives = 282/564 (50.00%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF-----IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGY--EAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
G ++CG D V+ P IRL G K EG++E+ ++ WG +CDD +T V+C+QLG+ + Y +GV I LD++ C G E I +C W KH+C +E + C D N R S P ++L++G GRVEI + GT+CDDG+TD +ASV+CR LG G A+A T FG G G I + N RC G E SL C G H C H EDAGVIC I + ++ ++++ L CG + +R R++GG +V G +PWQV +R + D CG +++S W+++A+HC+++ N Y +R GD++ + YE+ I+++ ++ Y+ P D+D ALI++ + + HV P CLP P + C ++GWGDT + L A V L+ C+E Y+ + T RM CAG +D+CQGDSGGPLVC+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 234 GEHNCGHKEDA----GVSCTPLADGAIRLAGGKGSHEGRLEVFHKARWGTVCDDGWTDLNTQVVCRQLGYKNGKILLENHYAGSAGV---------IWLDDISCTGKESMISQCSRRDWGKHDCSHQEDVHIACHPD----NETHR---------------------PLSGPP-------LRLMDGETKKEGRVEIYLNGQWGTICDDGWTDKDASVVCRQLGYKGPAKARTMAYFGEGKGPIHVDNVRCTGYERSLADCIKQDIGTHNCRHSEDAGVICDYIIKK-------STFAANKELSPLVCGSRLQHRRPKRIIGGKNSVRGGWPWQVALRLKSSHGDGRLLCGATLISSCWVLTAAHCFKRYGNNTRSYVIRVGDYHTLVPEEYEEDMTIQQIIIHNDYR-PDGNDYDIALIRLHGTAEQCVQLSTHVLPACLPLRRERPQKTASNCYITGWGDTGRAYSRTLQQATVTLLPKRVCEERYRSQFTGRMLCAGSVKTQKHVDSCQGDSGGPLVCERSGGSWIVYGVTSWGYGCGVKDSPGVYTKVSAFIPWIKSVTNL 744 HSP 2 Score: 147.902 bits (372), Expect = 1.025e-35 Identity = 90/276 (32.61%), Postives = 132/276 (47.83%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVH-GSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
G DC D + P+ RL G + +EG++E+ + G WG +CDD++ ++A V+C+QLG+ +A H G GV F ILLDE++C G E SIEEC W +HNC +E AGV C + + DG +++L G S+ GR+E+ GTVCDDG+TD V+CR LG + + + + G I L + C G E+ + QC+ WG+H CSH+ED + C
Sbjct: 134 GYCDCKQAKDSVFIPA--------RLVGGSSPYEGRIEVYHSGHWGTVCDDQWDDADAEVVCRQLGYGGVAKAWSQAHFGEGVGF-------ILLDEVQCTGNELSIEECQRSPWGEHNCGHKEDAGVSC----------------------TPLADG-----------------AIRLAGGKGSHEGRLEVFHKARWGTVCDDGWTDLNTQVVCRQLGYK-NGKILLENHYAGSAGVIWLDDISCTGKESMISQCSRRDWGKHDCSHQEDVHIAC 354 HSP 3 Score: 109.768 bits (273), Expect = 9.291e-24 Identity = 57/128 (44.53%), Postives = 74/128 (57.81%), Query Frame = 2
Query: 2477 GKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
G DC D F+ +LV G Y GR+E+ S GTVCDD + D++A V+CR LG G A+A + FG GVG I+L +C GNE S+ +C WGEH C HKEDAGV C P+ + +I
Sbjct: 134 GYCDCKQAKDS----VFIPARLVGGSSPYEGRIEVYHSGHWGTVCDDQWDDADAEVVCRQLGYGGVAKAWSQAHFGEGVGFILLDEVQCTGNELSIEECQRSPWGEHNCGHKEDAGVSC-TPLADGAI 256 HSP 4 Score: 74.7146 bits (182), Expect = 7.043e-13 Identity = 54/155 (34.84%), Postives = 79/155 (50.97%), Query Frame = 2
Query: 1958 CTGKYKCSSGMCIALSKSCDGRNDCGDNSD---------EMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVIC 2395
CTGK M S+ G++DC D E + P ++ PP +RL + EG+VEI G+WG ICDD +T +A V+C+QLG+ + + FG+ + P I +D ++C G E S+ +C HNC+ E AGVIC
Sbjct: 324 CTGK----ESMISQCSRRDWGKHDCSHQEDVHIACHPDNETHRP-LSGPP--LRLMDGETKKEGRVEIYLNGQWGTICDDGWTDKDASVVCRQLGYKGPAKAR--TMAYFGEGKGP-IHVDNVRCTGYERSLADCIKQDIGTHNCRHSEDAGVIC 468 HSP 5 Score: 65.0846 bits (157), Expect = 7.639e-10 Identity = 38/85 (44.71%), Postives = 51/85 (60.00%), Query Frame = 2
Query: 296 RSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
R G S+ EGR+EVY+S WG VCDD W+ DA V C+QLG+ A+ A Q H G G ++D+++C G+E SI EC
Sbjct: 150 RLVGGSSPYEGRIEVYHSG--HWGTVCDDQWDDADAEVVCRQLGYGGVAK-AWSQAHFGEG---VGFILLDEVQCTGNELSIEEC 228 HSP 6 Score: 54.299 bits (129), Expect = 1.376e-6 Identity = 35/94 (37.23%), Postives = 52/94 (55.32%), Query Frame = 2
Query: 269 PETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P + LR+ DG++ EGRVE+Y +WG +CDD W DA+V C+QLG+ A+ T + G G K +D ++C G E S+ +C
Sbjct: 363 PLSGPPLRLM-DGETK-KEGRVEIY--LNGQWGTICDDGWTDKDASVVCRQLGYKGPAKART-MAYFGEG---KGPIHVDNVRCTGYERSLADC 448
BLAST of Neurotrypsin vs. Ensembl Xenopus
Match: tmem132a (transmembrane protein 132A [Source:Xenbase;Acc:XB-GENE-1009879]) HSP 1 Score: 301.982 bits (772), Expect = 1.463e-86 Identity = 191/561 (34.05%), Postives = 282/561 (50.27%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEA--LEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGY--EAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
G ++CG D V+ P IRL G K EG++E+ ++ WG +CDD +T V+C+QLG+ L H +G +I LD++ C G E I +C W KH+C +E + C D N R S P ++L++G GRVEI + GT+CDDG+TD +ASV+CR LG G A+A T FG G G I + N RC G E SL C G H C H EDAGVIC I + ++ ++++ L CG + +R R++GG +V G +PWQV +R + D CG +++S W+++A+HC+++ N Y +R GD++ + YE+ I+++ ++ Y+ P D+D ALI++ + + HV P CLP P + C ++GWGDT + L A V L+ C+E Y+ + T RM CAG +D+CQGDSGGPLVC+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 341 GEHNCGHKED----AGVSCTPLADGAIRLAGGKGSHEGRLEVFHKARWGTVCDDGWTDLNTQVVCRQLGYKNGKILLENHYAGSA------GVIWLDDISCTGKESMISQCSRRDWGKHDCSHQEDVHIACHPD----NETHR---------------------PLSGPP-------LRLMDGETKKEGRVEIYLNGQWGTICDDGWTDKDASVVCRQLGYKGPAKARTMAYFGEGKGPIHVDNVRCTGYERSLADCIKQDIGTHNCRHSEDAGVICDYIIKK-------STFAANKELSPLVCGSRLQHRRPKRIIGGKNSVRGGWPWQVALRLKSSHGDGRLLCGATLISSCWVLTAAHCFKRYGNNTRSYVIRVGDYHTLVPEEYEEDMTIQQIIIHNDYR-PDGNDYDIALIRLHGTAEQCVQLSTHVLPACLPLRRERPQKTASNCYITGWGDTGRAYSRTLQQATVTLLPKRVCEERYRSQFTGRMLCAGSVKTQKHVDSCQGDSGGPLVCERSGGSWIVYGVTSWGYGCGVKDSPGVYTKVSAFIPWIKSVTNL 851 HSP 2 Score: 147.132 bits (370), Expect = 2.820e-35 Identity = 88/261 (33.72%), Postives = 128/261 (49.04%), Query Frame = 2
Query: 2063 SVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVH-GSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
SV P RL G + +EG++E+ + G WG +CDD++ ++A V+C+QLG+ +A H G GV F ILLDE++C G E SIEEC W +HNC +E AGV C + + DG +++L G S+ GR+E+ GTVCDDG+TD V+CR LG + + + + G I L + C G E+ + QC+ WG+H CSH+ED + C
Sbjct: 251 SVFIPA---RLVGGSSPYEGRIEVYHSGHWGTVCDDQWDDADAEVVCRQLGYGGVAKAWSQAHFGEGVGF-------ILLDEVQCTGNELSIEECQRSPWGEHNCGHKEDAGVSC----------------------TPLADG-----------------AIRLAGGKGSHEGRLEVFHKARWGTVCDDGWTDLNTQVVCRQLGYK-NGKILLENHYAGSAGVIWLDDISCTGKESMISQCSRRDWGKHDCSHQEDVHIAC 461 HSP 3 Score: 147.132 bits (370), Expect = 3.076e-35 Identity = 91/259 (35.14%), Postives = 127/259 (49.03%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKV-EIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
+RL GSK+ EG V E+ G WG IC ++ ++A VIC+QL E + + V +++C+G E +I +C D+WR C + A V C V +A KD F+ +LV G Y GR+E+ S GTVCDD + D++A V+CR LG G A+A + FG GVG I+L +C GNE S+ +C WGEH C HKEDAGV C P+ + +I
Sbjct: 143 VRLRGSKSKSEGSVVEVYVNGAWGTICSGQWDDNDASVICRQL---ELGDKGTAKKIVLSGVGQISAFWTDVQCRGNEENILQCAKDIWRSGACPQDTAAAVTCSVIQA---------KDSV-------------------------FIPARLVGGSSPYEGRIEVYHSGHWGTVCDDQWDDADAEVVCRQLGYGGVAKAWSQAHFGEGVGFILLDEVQCTGNELSIEECQRSPWGEHNCGHKEDAGVSC-TPLADGAI 363 HSP 4 Score: 74.7146 bits (182), Expect = 8.364e-13 Identity = 54/155 (34.84%), Postives = 79/155 (50.97%), Query Frame = 2
Query: 1958 CTGKYKCSSGMCIALSKSCDGRNDCGDNSD---------EMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVIC 2395
CTGK M S+ G++DC D E + P ++ PP +RL + EG+VEI G+WG ICDD +T +A V+C+QLG+ + + FG+ + P I +D ++C G E S+ +C HNC+ E AGVIC
Sbjct: 431 CTGK----ESMISQCSRRDWGKHDCSHQEDVHIACHPDNETHRP-LSGPP--LRLMDGETKKEGRVEIYLNGQWGTICDDGWTDKDASVVCRQLGYKGPAKAR--TMAYFGEGKGP-IHVDNVRCTGYERSLADCIKQDIGTHNCRHSEDAGVIC 575 HSP 5 Score: 64.6994 bits (156), Expect = 8.310e-10 Identity = 38/85 (44.71%), Postives = 51/85 (60.00%), Query Frame = 2
Query: 296 RSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
R G S+ EGR+EVY+S WG VCDD W+ DA V C+QLG+ A+ A Q H G G ++D+++C G+E SI EC
Sbjct: 257 RLVGGSSPYEGRIEVYHSG--HWGTVCDDQWDDADAEVVCRQLGYGGVAK-AWSQAHFGEG---VGFILLDEVQCTGNELSIEEC 335 HSP 6 Score: 54.299 bits (129), Expect = 1.458e-6 Identity = 35/94 (37.23%), Postives = 52/94 (55.32%), Query Frame = 2
Query: 269 PETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P + LR+ DG++ EGRVE+Y +WG +CDD W DA+V C+QLG+ A+ T + G G K +D ++C G E S+ +C
Sbjct: 470 PLSGPPLRLM-DGETK-KEGRVEIY--LNGQWGTICDDGWTDKDASVVCRQLGYKGPAKART-MAYFGEG---KGPIHVDNVRCTGYERSLADC 555 HSP 7 Score: 51.6026 bits (122), Expect = 9.208e-6 Identity = 36/126 (28.57%), Postives = 56/126 (44.44%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRV-EIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPG 2905
SV+L G V E+ + + GT+C + D++ASVICR L + + A G G ++ +CRGNE +++QC W C A V C + + S+ A + S G
Sbjct: 142 SVRLRGSKSKSEGSVVEVYVNGAWGTICSGQWDDNDASVICRQLELGDKGTAKKIVLSGVGQISAFWTDVQCRGNEENILQCAKDIWRSGACPQDTAAAVTCSVIQAKDSVFIPARLVGGSSPYEG 267
BLAST of Neurotrypsin vs. Ensembl Mouse
Match: Prss12 (protease, serine 12 neurotrypsin (motopsin) [Source:MGI Symbol;Acc:MGI:1100881]) HSP 1 Score: 309.686 bits (792), Expect = 2.753e-90 Identity = 192/561 (34.22%), Postives = 289/561 (51.52%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
KS G ++CG D ++C V IRL G K+ EG++E+ Y+G+WG +CDD +T +V C+ LGF +Y S V + I LD++ C G E S +C W +H+C E G+ C D SD ++LV+G + GRVE+ + GT+CDDG+TD A+VICR LG G A+A T FG G G I + N +C GNE +L C G H C H EDAGVIC LE +++ ++++ CG + + +R+ R++GG ++ GA+PWQ +R R D CG ++LS W+++A+HC+++ N Y VR GD++ + +EQ ++++ ++ Y+ P D+D AL++++ + HV P CLP P + C ++GWGDT + L A VPL+ CKE YK T RM CAG E +D+CQGDSGGPL+C+ + + ++G TSWG GC V +PGVY +V + + ++K T
Sbjct: 247 KSSWGEHNCGHKEDAGVSC--VPLTDGVIRLAGGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGF----KYGKQSSVNHFDGSNRPIWLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTCYPD--------------------------------SDGHRLSPGFPIRLVDGENKKEGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHMDNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDY--LEKKASSSG-----NKEMLSSGCGLRLLHRRQKRIIGGNNSLRGAWPWQASLRLRSAHGDGRLLCGATLLSSCWVLTAAHCFKRYGNNSRSYAVRVGDYHTLVPEEFEQEIGVQQIVIHRNYR-PDRSDYDIALVRLQGPGEQCARLSTHVLPACLPLWRERPQKTASNCHITGWGDTGRAYSRTLQQAAVPLLPKRFCKERYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKPDESWVVYGVTSWGYGCGVKDTPGVYTRVPAFVPWIKSVTSL 761 HSP 2 Score: 149.443 bits (376), Expect = 2.868e-36 Identity = 89/249 (35.74%), Postives = 125/249 (50.20%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G + EG+VE+ + G+WG ICDD++ ++A VIC+QLG + H + FG+ PI LLDE++C G E SIE+CP W +HNC +E AGV C + DG ++L G ++ GR+E+ GTVCDDG+T+ V CR+LG Q+ + GS I L + C G E S +QC+ +WG H CSH+ED G+ C
Sbjct: 166 IRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQA--HFGEGSGPI-LLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------VPLTDG-----------------VIRLAGGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGFKYGKQSSVNHFDGSNR-PIWLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTC 371 HSP 3 Score: 106.686 bits (265), Expect = 6.995e-23 Identity = 58/128 (45.31%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 2477 GKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
GKVD D +G ++LV G + GRVE+ + GT+CDD + +++A VICR LG+SG A+A FG G G I+L RC GNE S+ QC WGEH C HKEDAGV C +P+ + I
Sbjct: 148 GKVDWG-YCDCGQGPALPVIRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQAHFGEGSGPILLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC-VPLTDGVI 273 HSP 4 Score: 67.0106 bits (162), Expect = 1.411e-10 Identity = 36/93 (38.71%), Postives = 56/93 (60.22%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+ P IR G ++ +EGRVE+Y++ +WG +CDD W+ DA+V C+QLG A+ A Q H G G ++D+++C G+E SI +C
Sbjct: 159 QGPALPVIRLVGGNSGHEGRVELYHAG--QWGTICDDQWDNADADVICRQLGLSGIAK-AWHQAHFGEG---SGPILLDEVRCTGNELSIEQC 245 HSP 5 Score: 53.9138 bits (128), Expect = 1.404e-6 Identity = 39/106 (36.79%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 233 TIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
T YP +P P RL DG++ EGRVEV+ +WG +CDD W A V C+QLG+ AR T + G G K +D +KC G+E ++ +C
Sbjct: 370 TCYPDSDGHRLSPGFPIRL---VDGENK-KEGRVEVF--VNGQWGTICDDGWTDKHAAVICRQLGYKGPARART-MAYFGEG---KGPIHMDNVKCTGNEKALADC 465
BLAST of Neurotrypsin vs. Ensembl Mouse
Match: Tmprss2 (transmembrane protease, serine 2 [Source:MGI Symbol;Acc:MGI:1354381]) HSP 1 Score: 196.438 bits (498), Expect = 1.858e-53 Identity = 137/426 (32.16%), Postives = 206/426 (48.36%), Query Frame = 2
Query: 2402 DRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGR---AQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKN--RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLV--ETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
D C+ +E C TCI S CDG C + D E +C V+L S+ +V + + VC D +++S C+ +G +Q + D + ++ +S+ GN V + CS + + C + CG + + +R+ R+VGG G +PWQV + H G +H CGGSI++ +WI++A+HC E+P + RY+ + Y ++E++ +P Y T + D AL + FND V+PVCLP PGM+ DL +C +SGWG T +T++ L AA VPLI+ +C K Y + IT M CAG+ G +D+CQGDSGGPLV NGI+ + G TSWG GCA PGVY V D++ ++
Sbjct: 109 DSNCSTSEMECGSSGTCISSSLWCDGVAHCPNGED--ENRC---VRLYG--QSFILQVYSSQRKAWYPVCQDDWSESYGRAACKDMGYKNNFYSSQGIPDQSGATSFMKLNVSS----GN----VDLYKKLYHSDSCSSRMVVSLRC------------------------IECGVRSV-KRQSRIVGGLNASPGDWPWQVSL-HVQG---VHVCGGSIITPEWIVTAAHCVEEPLSSPRYWTAFAGILRQSLMFYGSRHQVEKVISHPNYDS-KTKNNDIAL----MKLQTPLAFNDLVKPVCLPNPGMMLDLDQECWISGWGATYEKGKTSDVLNAAMVPLIEPSKCNSKYIYNNLITPAMICAGFLQGSVDSCQGDSGGPLVTLKNGIWWLIGDTSWGSGCAKALRPGVYGNVTVFTDWIYQQ 485
BLAST of Neurotrypsin vs. Ensembl Mouse
Match: Tmprss15 (transmembrane protease, serine 15 [Source:MGI Symbol;Acc:MGI:1197523]) HSP 1 Score: 190.66 bits (483), Expect = 9.881e-49 Identity = 129/440 (29.32%), Postives = 207/440 (47.05%), Query Frame = 2
Query: 2351 RKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEK----PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVE---TAEKLLAAEVPLIKAERCKEFYKD-RITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
R+ K+ +G + C + EF+C KD CI + +CD C D SDE C V+ +NG S +G V+ C + +T ++ +C +LG+ ++ + G G V P T +QC+ +C+HK +CG K +TQ+ ++VGG + GA+PW V + HR D CG S++S DW++SA+HC + P V +N ++++ +NP Y + D A++ ++ + D++QP+CLP+ + C ++GWG + T + L A+VPLI E+C++ + IT+ M CAGYE GGID+CQGDSGGPL+CQ N + + G TS+G CA+P PGVY +V ++++
Sbjct: 668 RRKGFKANFTSGYYLGIPEPCQDDEFQC-KDGNCIPLGNLCDSYPHCRDGSDE--ASC---VRFLNGTRSNNGLVQFNIHSIWHIACAENWTTQISNEVCHLLGLGSANSSMPISSTGGGPFVRVNQAPNGSLILTPSLQCSQDSLILLQCNHK--------------------------------SCGEKKVTQKVSPKIVGGSDAQAGAWPWVVALYHRDRSTDRLLCGASLVSSDWLVSAAHCVYRRNLDPTRWTAVLGLHMQSNLTSPQVVRRVVDQIVINPHYDRRRKVN-DIAMMHLEFKVN----YTDYIQPICLPEENQIFIPGRTCSIAGWGYDKINAGSTVDVLKEADVPLISNEKCQQQLPEYNITESMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLVGVTSFGVQCALPNHPGVYVRVSQFIEWI 1064
BLAST of Neurotrypsin vs. Ensembl Mouse
Match: Tmprss15 (transmembrane protease, serine 15 [Source:MGI Symbol;Acc:MGI:1197523]) HSP 1 Score: 190.66 bits (483), Expect = 1.054e-48 Identity = 129/440 (29.32%), Postives = 207/440 (47.05%), Query Frame = 2
Query: 2351 RKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEK----PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVE---TAEKLLAAEVPLIKAERCKEFYKD-RITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
R+ K+ +G + C + EF+C KD CI + +CD C D SDE C V+ +NG S +G V+ C + +T ++ +C +LG+ ++ + G G V P T +QC+ +C+HK +CG K +TQ+ ++VGG + GA+PW V + HR D CG S++S DW++SA+HC + P V +N ++++ +NP Y + D A++ ++ + D++QP+CLP+ + C ++GWG + T + L A+VPLI E+C++ + IT+ M CAGYE GGID+CQGDSGGPL+CQ N + + G TS+G CA+P PGVY +V ++++
Sbjct: 653 RRKGFKANFTSGYYLGIPEPCQDDEFQC-KDGNCIPLGNLCDSYPHCRDGSDE--ASC---VRFLNGTRSNNGLVQFNIHSIWHIACAENWTTQISNEVCHLLGLGSANSSMPISSTGGGPFVRVNQAPNGSLILTPSLQCSQDSLILLQCNHK--------------------------------SCGEKKVTQKVSPKIVGGSDAQAGAWPWVVALYHRDRSTDRLLCGASLVSSDWLVSAAHCVYRRNLDPTRWTAVLGLHMQSNLTSPQVVRRVVDQIVINPHYDRRRKVN-DIAMMHLEFKVN----YTDYIQPICLPEENQIFIPGRTCSIAGWGYDKINAGSTVDVLKEADVPLISNEKCQQQLPEYNITESMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLVGVTSFGVQCALPNHPGVYVRVSQFIEWI 1049
BLAST of Neurotrypsin vs. Ensembl Mouse
Match: F11 (coagulation factor XI [Source:MGI Symbol;Acc:MGI:99481]) HSP 1 Score: 174.866 bits (442), Expect = 3.818e-45 Identity = 98/248 (39.52%), Postives = 147/248 (59.27%), Query Frame = 2
Query: 2930 TQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCY---EKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLV--ETAEKLLAAEVPLIKAERCKEFYK-DRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
T+ RVVGG +V+G +PWQV + G H CGGSI+ WI++A+HC+ E PK + V G N +E++ F ++E+ ++ +Y + +D AL+K++ + D +P+CLP G + T+C V+GWG T + E L A+VPL+ E C+ Y+ +IT++M CAGY+ GG DTC+GDSGGPL C+ NG++ + G TSWG+GC PGVY V +D++ EKT
Sbjct: 384 TKINPRVVGGAASVHGEWPWQVTLHISQG----HLCGGSIIGNQWILTAAHCFSGIETPK-KLRVYGGIVNQSEINEGTAFFRVQEMIIHDQYTTAES-GYDIALLKLESAMN----YTDFQRPICLPSKGDRNAVHTECWVTGWGYTALRGEVQSTLQKAKVPLVSNEECQTRYRRHKITNKMICAGYKEGGKDTCKGDSGGPLSCKYNGVWHLVGITSWGEGCGQKERPGVYTNVAKYVDWILEKT 621
BLAST of Neurotrypsin vs. UniProt/SwissProt
Match: sp|Q5G265|NETR_SAGLB (Neurotrypsin OS=Saguinus labiatus OX=78454 GN=PRSS12 PE=3 SV=1) HSP 1 Score: 314.694 bits (805), Expect = 3.928e-90 Identity = 194/559 (34.70%), Postives = 289/559 (51.70%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKN---RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQVNG-IYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
KS G ++CG D ++C +T IRL G K EG++E+ Y G+WG +CDD +T +V+C+QLGF + S F + PI LD++ C G E +C W +H+C E G+ C S+ V+L++G + GRVE+ + GT+CDDG+TD +A+VICR LG G A+A T FG G G I + N +C GNE SL C G H C H EDAGVIC + S + SL CG + + +R+ R++GG ++ G +PWQV +R + D CG ++LS W+++A+HC+++ N Y VR GD++ + +E+ ++E+ ++ +Y+ P + D+D AL++++ FN HV P CLP P + C ++GWGDT + L A + L+ C+E YK R T RM CAG +E +D+CQGDSGGPL+C+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 361 KSSWGEHNCGHKEDAGVSCTPLT--DGVIRLAGGKGSHEGRLEVYYSGQWGTVCDDGWTELNTYVVCRQLGFKYGKQ---ASANHFEESAGPI-WLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVGIACYPG--------------------------------SEGHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKGPIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSS-------VCGLRLLHRRQKRIIGGKNSLRGGWPWQVSLRLKSSHRDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRNYAVRVGDYHTLVPEEFEEEIGVQEIVIHREYR-PDSSDYDIALVRLQGPEEQCARFNSHVLPACLPLWRERPQKTASNCYITGWGDTGQAYSRTLQQAAIHLLPKRFCEERYKGRFTGRMLCAGNLHEHKHVDSCQGDSGGPLMCERPGESWVVYGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVT 873 HSP 2 Score: 152.525 bits (384), Expect = 3.530e-36 Identity = 91/254 (35.83%), Postives = 128/254 (50.39%), Query Frame = 2
Query: 2075 PPFEI-RLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
P F I RL G + EG+VE+ + G+WG ICDD++ ++A VIC+QL + H + FG+ P+ +LDE++C G E SIE+CP W +HNC +E AGV C + + DG ++L G S+ GR+E+ S GTVCDDG+T+ V+CR LG QA + F G I L + C G ET +QC+ +WG H CSH+ED G+ C
Sbjct: 275 PAFPIIRLVGGSSVHEGRVELYHAGQWGTICDDQWDDADAEVICRQLSLSGIAKAWHQA--YFGEGSGPV-MLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------TPLTDG-----------------VIRLAGGKGSHEGRLEVYYSGQWGTVCDDGWTELNTYVVCRQLGFKYGKQASAN-HFEESAGPIWLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVGIAC 485 HSP 3 Score: 142.895 bits (359), Expect = 3.425e-33 Identity = 86/253 (33.99%), Postives = 119/253 (47.04%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVK----FGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL GSKN++EG VE+ G WG +C + S+A VIC QL + G G+ F + + ++C+G E +I C D+W+ C + A V+C G F ++LV G + GRVE+ + GT+CDD + D++A VICR L +SG A+A FG G G ++L RC GNE S+ QC WGEH C HKEDAGV C
Sbjct: 170 VRLRGSKNEFEGTVEVYANGVWGTVCSSHWDDSDASVICHQL-------QLGGKGIAKQTPFSGLGLIPVYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVMCSFS------------------------------------HGPAFPIIRLVGGSSVHEGRVELYHAGQWGTICDDQWDDADAEVICRQLSLSGIAKAWHQAYFGEGSGPVMLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC 379 HSP 4 Score: 63.5438 bits (153), Expect = 1.164e-8 Identity = 36/101 (35.64%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
SV+L + + G VE+ + GTVC + DS+ASVIC L + G+ A G G+ + SN RCRG+E +++ C W C K A V+C
Sbjct: 169 SVRLRGSKNEFEGTVEVYANGVWGTVCSSHWDDSDASVICHQLQLGGKGIAKQTPFSGLGLIPVYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVMC 269 HSP 5 Score: 62.3882 bits (150), Expect = 2.611e-8 Identity = 34/86 (39.53%), Postives = 52/86 (60.47%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G S+ +EGRVE+Y++ +WG +CDD W+ DA V C+QL A+ A Q + G G ++D+++C G+E SI +C
Sbjct: 280 IRLVGGSSVHEGRVELYHAG--QWGTICDDQWDDADAEVICRQLSLSGIAK-AWHQAYFGEG---SGPVMLDEVRCTGNELSIEQC 359 HSP 6 Score: 55.8398 bits (133), Expect = 2.912e-6 Identity = 32/76 (42.11%), Postives = 43/76 (56.58%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+ +WG +CDD W DA V C+QLG+ AR T + G G K +D +KC G+E S+ +C
Sbjct: 510 EGRVEVF--INGQWGTICDDGWTDKDAAVICRQLGYKGPARART-MAYFGEG---KGPIHVDNVKCTGNERSLADC 579
BLAST of Neurotrypsin vs. UniProt/SwissProt
Match: sp|Q5G267|NETR_MACMU (Neurotrypsin OS=Macaca mulatta OX=9544 GN=PRSS12 PE=3 SV=1) HSP 1 Score: 313.153 bits (801), Expect = 1.343e-89 Identity = 193/559 (34.53%), Postives = 291/559 (52.06%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKN---RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQVNG-IYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
KS G ++CG D ++C +T IRL G K EG++E+ Y+G+WG +CDD +T +V+C+QLGF + S F + PI LD++ C G E +C W +H+C E D + C S+ V+L++G + GRVE+ + GT+CDDG+TD +A+VICR LG G A+A T FG G G I + N +C GNE SL C G H C H EDAGVIC + S + SL CG + + +R+ R++GG ++ G +PWQV +R + D CG ++LS W+++A+HC+++ N Y VR GD++ + +E+ ++++ ++ +Y+ P + D+D AL++++ F+ HV P CLP P + C ++GWGDT + L A +PL+ C+E YK R T RM CAG +E +D+CQGDSGGPL+C+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 361 KSSWGEHNCGHKEDAGVSCTPLT--DGVIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQ---ASANHFEESTGPI-WLDDVSCSGKETRFLQCSRRQWGQHDCSHHE--------------------------------DVSIACYPGSEGHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKGPIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSS-------VCGLRLLHRRQKRIIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRNYAVRVGDYHTLVPEEFEEEIGVQQIVIHREYR-PDSSDYDIALVRLQGPEEQCARFSSHVLPACLPFWRERPQKTASNCYITGWGDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWAVYGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVT 873 HSP 2 Score: 150.599 bits (379), Expect = 1.291e-35 Identity = 91/262 (34.73%), Postives = 129/262 (49.24%), Query Frame = 2
Query: 2051 MNCPSVTFPPFEI-RLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+ C P F I RL G + EG+VE+ + G+WG ICDD++ ++A VIC+QLG + H + FG+ P+ +LDE++C G E SIE+CP W +HNC +E AGV C + + DG ++L G S+ GR+E+ GTVCDDG+T+ V+CR LG QA + F G I L + C G ET +QC+ +WG+H CSH ED + C
Sbjct: 267 VTCSFSRGPAFPIIRLVGGSSVHEGRVELYHAGQWGTICDDQWDDADAEVICRQLGLSGIAKAWHQA--YFGEGSGPV-MLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------TPLTDG-----------------VIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQASAN-HFEESTGPIWLDDVSCSGKETRFLQCSRRQWGQHDCSHHEDVSIAC 485 HSP 3 Score: 145.591 bits (366), Expect = 4.583e-34 Identity = 88/253 (34.78%), Postives = 119/253 (47.04%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVK----FGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G KN++EG VE+ G WG +C + S+A VIC QL + G G+ F + I ++C+G E +I C D+W+ C + A V C R G F ++LV G + GRVE+ + GT+CDD + D++A VICR LG+SG A+A FG G G ++L RC GNE S+ QC WGEH C HKEDAGV C
Sbjct: 170 VRLRGGKNEFEGTVEVYASGAWGTVCSSHWDDSDASVICHQL-------QLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTCSFSR------------------------------------GPAFPIIRLVGGSSVHEGRVELYHAGQWGTICDDQWDDADAEVICRQLGLSGIAKAWHQAYFGEGSGPVMLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC 379 HSP 4 Score: 67.781 bits (164), Expect = 5.412e-10 Identity = 39/103 (37.86%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGL 2839
SV+L G + + G VE+ S + GTVC + DS+ASVIC L + G+ A G G+ I SN RCRG+E +++ C W C K A V C
Sbjct: 169 SVRLRGGKNEFEGTVEVYASGAWGTVCSSHWDDSDASVICHQLQLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTCSF 271 HSP 5 Score: 64.6994 bits (156), Expect = 4.770e-9 Identity = 35/86 (40.70%), Postives = 53/86 (61.63%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G S+ +EGRVE+Y++ +WG +CDD W+ DA V C+QLG A+ A Q + G G ++D+++C G+E SI +C
Sbjct: 280 IRLVGGSSVHEGRVELYHAG--QWGTICDDQWDDADAEVICRQLGLSGIAK-AWHQAYFGEG---SGPVMLDEVRCTGNELSIEQC 359 HSP 6 Score: 55.8398 bits (133), Expect = 2.987e-6 Identity = 32/76 (42.11%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+ + +WG +CDD W DA V C+QLG+ AR T + G G K +D +KC G+E S+ +C
Sbjct: 510 EGRVEVFING--QWGTICDDGWTDKDAAVICRQLGYKGPARART-MAYFGEG---KGPIHVDNVKCTGNERSLADC 579
BLAST of Neurotrypsin vs. UniProt/SwissProt
Match: sp|O08762|NETR_MOUSE (Neurotrypsin OS=Mus musculus OX=10090 GN=Prss12 PE=1 SV=1) HSP 1 Score: 309.686 bits (792), Expect = 1.925e-89 Identity = 192/561 (34.22%), Postives = 289/561 (51.52%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
KS G ++CG D ++C V IRL G K+ EG++E+ Y+G+WG +CDD +T +V C+ LGF +Y S V + I LD++ C G E S +C W +H+C E G+ C D SD ++LV+G + GRVE+ + GT+CDDG+TD A+VICR LG G A+A T FG G G I + N +C GNE +L C G H C H EDAGVIC LE +++ ++++ CG + + +R+ R++GG ++ GA+PWQ +R R D CG ++LS W+++A+HC+++ N Y VR GD++ + +EQ ++++ ++ Y+ P D+D AL++++ + HV P CLP P + C ++GWGDT + L A VPL+ CKE YK T RM CAG E +D+CQGDSGGPL+C+ + + ++G TSWG GC V +PGVY +V + + ++K T
Sbjct: 247 KSSWGEHNCGHKEDAGVSC--VPLTDGVIRLAGGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGF----KYGKQSSVNHFDGSNRPIWLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTCYPD--------------------------------SDGHRLSPGFPIRLVDGENKKEGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHMDNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDY--LEKKASSSG-----NKEMLSSGCGLRLLHRRQKRIIGGNNSLRGAWPWQASLRLRSAHGDGRLLCGATLLSSCWVLTAAHCFKRYGNNSRSYAVRVGDYHTLVPEEFEQEIGVQQIVIHRNYR-PDRSDYDIALVRLQGPGEQCARLSTHVLPACLPLWRERPQKTASNCHITGWGDTGRAYSRTLQQAAVPLLPKRFCKERYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKPDESWVVYGVTSWGYGCGVKDTPGVYTRVPAFVPWIKSVTSL 761 HSP 2 Score: 149.443 bits (376), Expect = 2.006e-35 Identity = 89/249 (35.74%), Postives = 125/249 (50.20%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G + EG+VE+ + G+WG ICDD++ ++A VIC+QLG + H + FG+ PI LLDE++C G E SIE+CP W +HNC +E AGV C + DG ++L G ++ GR+E+ GTVCDDG+T+ V CR+LG Q+ + GS I L + C G E S +QC+ +WG H CSH+ED G+ C
Sbjct: 166 IRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQA--HFGEGSGPI-LLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------VPLTDG-----------------VIRLAGGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGFKYGKQSSVNHFDGSNR-PIWLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTC 371 HSP 3 Score: 106.686 bits (265), Expect = 4.893e-22 Identity = 58/128 (45.31%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 2477 GKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
GKVD D +G ++LV G + GRVE+ + GT+CDD + +++A VICR LG+SG A+A FG G G I+L RC GNE S+ QC WGEH C HKEDAGV C +P+ + I
Sbjct: 148 GKVDWG-YCDCGQGPALPVIRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQAHFGEGSGPILLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC-VPLTDGVI 273 HSP 4 Score: 67.0106 bits (162), Expect = 9.870e-10 Identity = 36/93 (38.71%), Postives = 56/93 (60.22%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+ P IR G ++ +EGRVE+Y++ +WG +CDD W+ DA+V C+QLG A+ A Q H G G ++D+++C G+E SI +C
Sbjct: 159 QGPALPVIRLVGGNSGHEGRVELYHAG--QWGTICDDQWDNADADVICRQLGLSGIAK-AWHQAHFGEG---SGPILLDEVRCTGNELSIEQC 245 HSP 5 Score: 53.9138 bits (128), Expect = 9.824e-6 Identity = 39/106 (36.79%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 233 TIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
T YP +P P RL DG++ EGRVEV+ +WG +CDD W A V C+QLG+ AR T + G G K +D +KC G+E ++ +C
Sbjct: 370 TCYPDSDGHRLSPGFPIRL---VDGENK-KEGRVEVF--VNGQWGTICDDGWTDKHAAVICRQLGYKGPARART-MAYFGEG---KGPIHMDNVKCTGNEKALADC 465
BLAST of Neurotrypsin vs. UniProt/SwissProt
Match: sp|Q5G268|NETR_NOMLE (Neurotrypsin OS=Nomascus leucogenys OX=61853 GN=PRSS12 PE=3 SV=1) HSP 1 Score: 312.383 bits (799), Expect = 1.989e-89 Identity = 192/559 (34.35%), Postives = 290/559 (51.88%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKN---RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQVNG-IYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
KS G ++CG D ++C +T IRL G K EG++E+ Y+G+WG +CDD +T +V+C+QLGF + S F + PI LD++ C G E +C W +H+C E D + C + V+L++G + GRVE+ + GT+CDDG+TD +A+VICR LG G A+A T FG G G I + N +C GNE SL C G H C H EDAGVIC + S + SL CG + + +R+ R++GG ++ G +PWQV +R + D CG ++LS W+++A+HC+++ N Y VR GD++ + +E+ ++++ ++ +Y+ P + D+D AL++++ F+ HV P CLP P + C ++GWGDT + L A +PL+ C+E YK R T RM CAG +E +D+CQGDSGGPL+C+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 361 KSSWGEHNCGHKEDAGVSCTPLT--DGVIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQ---ASANHFEESTGPI-WLDDVSCSGKETRFLQCSRRQWGRHDCSHRE--------------------------------DVSIACYPGGEGHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKGPIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSS-------VCGLRLLHRRQKRIIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRNYAVRVGDYHTLVPEEFEEEIGVQQIVIHREYR-PDSSDYDIALVRLQGPEEQCARFSSHVLPACLPLWRERPQKTASNCYITGWGDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWVVYGVTSWGHGCGVKDSPGVYTKVSAFVPWIKSVT 873 HSP 2 Score: 150.984 bits (380), Expect = 1.066e-35 Identity = 90/255 (35.29%), Postives = 128/255 (50.20%), Query Frame = 2
Query: 2069 TFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
TFP IRL G + EG+VE+ + G+WG +CDD++ ++A VIC+QLG + H + FG+ P+ +LDE++C G E SIE+CP W +HNC +E AGV C + + DG ++L G S+ GR+E+ GTVCDDG+T+ V+CR LG QA + F G I L + C G ET +QC+ +WG H CSH+ED + C
Sbjct: 276 TFPI--IRLVGGSSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQA--YFGEGSGPV-MLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------TPLTDG-----------------VIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVVCRQLGFKYGKQASAN-HFEESTGPIWLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVSIAC 485 HSP 3 Score: 144.05 bits (362), Expect = 1.570e-33 Identity = 89/253 (35.18%), Postives = 118/253 (46.64%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVK----FGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G KN++EG VE+ G WG +C + S+A VIC QL + G G+ F + I ++C+G E +I C D+W+ C + A V C G F ++LV G + GRVE+ + GTVCDD + D++A VICR LG+SG A+A FG G G ++L RC GNE S+ QC WGEH C HKEDAGV C
Sbjct: 170 IRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQL-------QLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTCSFS------------------------------------HGPTFPIIRLVGGSSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQAYFGEGSGPVMLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC 379 HSP 4 Score: 66.6254 bits (161), Expect = 1.451e-9 Identity = 38/101 (37.62%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
S++L G + + G VE+ S GTVC + DS+ASVIC L + G+ A G G+ I SN RCRG+E +++ C W C K A V C
Sbjct: 169 SIRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQLQLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTC 269 HSP 5 Score: 65.0846 bits (157), Expect = 3.543e-9 Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G S+ +EGRVE+Y++ +WG VCDD W+ DA V C+QLG A+ A Q + G G ++D+++C G+E SI +C
Sbjct: 280 IRLVGGSSVHEGRVELYHAG--QWGTVCDDQWDDADAEVICRQLGLSGIAK-AWHQAYFGEG---SGPVMLDEVRCTGNELSIEQC 359 HSP 6 Score: 55.4546 bits (132), Expect = 3.012e-6 Identity = 32/76 (42.11%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+ + +WG +CDD W DA V C+QLG+ AR T + G G K +D +KC G+E S+ +C
Sbjct: 510 EGRVEVFING--QWGTICDDGWTDKDAAVICRQLGYKGPARART-MAYFGEG---KGPIHVDNVKCTGNERSLADC 579
BLAST of Neurotrypsin vs. UniProt/SwissProt
Match: sp|Q5G266|NETR_TRAPH (Neurotrypsin OS=Trachypithecus phayrei OX=61618 GN=PRSS12 PE=3 SV=1) HSP 1 Score: 312.383 bits (799), Expect = 2.173e-89 Identity = 193/559 (34.53%), Postives = 290/559 (51.88%), Query Frame = 2
Query: 2006 KSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEKPKN---RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPD-LKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQVNG-IYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
KS G ++CG D ++C +T IRL G K EG++E+ Y+G+WG +CDD +T +V C+QLGF + S F + PI LD++ C G E +C W +H+C E D + C S+ V+L++G + GRVE+ + GT+CDDG+TD +A+VICR LG G A+A T FG G G I + N +C GNE SL C G H C H EDAGVIC + S + SL CG + + +R+ R++GG ++ G +PWQV +R + D CG ++LS W+++A+HC+++ N Y VR GD++ + +E+ ++++ ++ +Y+ P + D+D AL++++ F+ HV P CLP P + C ++GWGDT + L A +PL+ C+E YK R T RM CAG +E +D+CQGDSGGPL+C+ G + ++G TSWG GC V SPGVY KV + + ++K T
Sbjct: 361 KSSWGEHNCGHKEDAGVSCTPLT--DGVIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVACRQLGFKYGKQ---ASANHFEESTGPI-WLDDVSCSGKETRFLQCSRRQWGRHDCSHRE--------------------------------DVSIACYPGSEGHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKGPIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSS-------VCGLRLLHRRQKRIIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRNYAVRVGDYHTLVPEEFEEEIGVQQIVIHREYR-PDSSDYDIALVRLQGPEEQCARFSSHVLPACLPFWRERPQKTASNCYITGWGDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWAVYGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVT 873 HSP 2 Score: 149.058 bits (375), Expect = 4.210e-35 Identity = 90/262 (34.35%), Postives = 128/262 (48.85%), Query Frame = 2
Query: 2051 MNCPSVTFPPFEI-RLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+ C P F I RL G + EG+VE+ + G+WG +CDD++ ++A VIC+QLG + H + FG+ P+ +LDE++C G E SIE+CP W +HNC +E AGV C + + DG ++L G S+ GR+E+ GTVCDDG+T+ V CR LG QA + F G I L + C G ET +QC+ +WG H CSH+ED + C
Sbjct: 267 VTCSFSHGPAFPIIRLVGGNSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQA--YFGEGSGPV-MLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC----------------------TPLTDG-----------------VIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELNTYVACRQLGFKYGKQASAN-HFEESTGPIWLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVSIAC 485 HSP 3 Score: 143.665 bits (361), Expect = 2.054e-33 Identity = 88/253 (34.78%), Postives = 118/253 (46.64%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVK----FGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G KN++EG VE+ G WG +C + S+A VIC QL + G G+ F + I ++C+G E +I C D+W+ C + A V C G F ++LV G + GRVE+ + GTVCDD + D++A VICR LG+SG A+A FG G G ++L RC GNE S+ QC WGEH C HKEDAGV C
Sbjct: 170 VRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQL-------QLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQXGVCPQKMAAAVTCSFS------------------------------------HGPAFPIIRLVGGNSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQAYFGEGSGPVMLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSC 379 HSP 4 Score: 67.3958 bits (163), Expect = 8.070e-10 Identity = 39/103 (37.86%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGL 2839
SV+L G + + G VE+ S GTVC + DS+ASVIC L + G+ A G G+ I SN RCRG+E +++ C W C K A V C
Sbjct: 169 SVRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQLQLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQXGVCPQKMAAAVTCSF 271 HSP 5 Score: 63.5438 bits (153), Expect = 1.088e-8 Identity = 35/86 (40.70%), Postives = 53/86 (61.63%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G ++ +EGRVE+Y++ +WG VCDD W+ DA V C+QLG A+ A Q + G G ++D+++C G+E SI +C
Sbjct: 280 IRLVGGNSVHEGRVELYHAG--QWGTVCDDQWDDADAEVICRQLGLSGIAK-AWHQAYFGEG---SGPVMLDEVRCTGNELSIEQC 359 HSP 6 Score: 55.4546 bits (132), Expect = 3.143e-6 Identity = 32/76 (42.11%), Postives = 44/76 (57.89%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+ + +WG +CDD W DA V C+QLG+ AR T + G G K +D +KC G+E S+ +C
Sbjct: 510 EGRVEVFING--QWGTICDDGWTDKDAAVICRQLGYKGPARART-MAYFGEG---KGPIHVDNVKCTGNERSLADC 579
BLAST of Neurotrypsin vs. TrEMBL
Match: A0A267FDY5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019787g1 PE=3 SV=1) HSP 1 Score: 763.451 bits (1970), Expect = 0.000e+0 Identity = 448/1170 (38.29%), Postives = 674/1170 (57.61%), Query Frame = 2
Query: 242 PIRSVLD----GAPETPERLRIRSD-GKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLG--SEASIFECXXXXXXX-XXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSD-KPSLPLVTELPRISDPPGKGNGGC-ADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADE--KNCSII-----NETEILVKYDKFK----SKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFS-ECHLMKQLQSEIP--DSLKHINYPGDHYSLKTDCT--GKYKCSSGMCIALSKSCDGRNDCGDNSDEM---NCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRK-HNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIV-LSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGL-TCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDI-HWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL-VETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
P+ ++L G P +P++ R+R + NEGRVEV T WGL C DY+ +ANV C+ LGF A + + G G + + D +I +KC ++++F+CSH + ++GV C +N GC +GW +++G+CY+V + +T + A + CK + +KL + + EN+F++N+LY++F+ I WTGG YA+ + W ++ TIH V + S LWF G+ E SD RP+G+ +K+SC+A+++++ S ++ D NYFWWDN+SC T+LP+ICQK+ T +DCY +G YRG +RT++GT C WS L VN THP GLG+HN CRNPD D PWCWV+ + V+GYC + C + + L T+L P + C + +Q++CPSM +Y C+ +VCD DC G EDE E CK C + +FYC+ CI S +CD Q C DE + C + ++ E+ Y K + S I + Y +L L +C +C + +F C + Y N F C L+ Q +++ D++ Y Y K C K++C + C+ + C+G +DCGD SDE+ +CP P F +RL+G + ++EG+VE++ EWG +CDD + V+C QLGF E + + K D L+ + C+GTE S+ +CPH W+ NC +AG++C DRACT+ EFRC CI +SQ+CDG C+D +DE E ++ L NG SG +EI R+ G VCDD F D+EA V+C LGI GR + + F + G ++ L + +C G+E+SL C WG C +E + CGLP + T T+ + TCG++ GR++ G+ + G FPWQVG+R +V + D CGG+I+++ WI++A+HC++ PK+ Y +R GD +N + +E+ F+I+ +F++P+Y G P++D+D LI+I+ ++ I FN VQP CLP+ + KT C +SGWG ++ L+AA VPLI RC + Y RITDRMQCAG+EAGGID+CQGDSGGPLVC ++G+YT+ GATSWG+GCA+P SPGVYA + ++ D++ E
Sbjct: 85 PVDTLLKYRYGGVPVSPQKARLRGELSHMNPNEGRVEVEYRGT--WGLACGDYFGREEANVYCRNLGFKKGANKPYGRSFFGTGNFKRWDILIGHVKCAAYRGQSNLFQCSHNLMPACSLNNTAGVECTYNEGCEFGWSAYDGLCYRVESGPMTAEDAAKACKAKGAKLVAVNSQGENSFITNMLYTKFQDIAAAWTGGQYARFRDQLE--YDAWTFKDTIHLVK--SDSKLWFPGFTE--SDARPIGNKEKQSCIAISKIFYKSSERRDV-DTNYFWWDNISCKTELPYICQKLGTL-TSDCYSQSGGNYRGEAARTDKGTPCQPWSENRL-VNSMTHPGQGLGEHNLCRNPDGDTKPWCWVDNERNVFGYCSLRLCGEGEGGDGLPTDLK-----PSYNHSRCDSPDQYFCPSMINYRPTCVDRRQVCDSHKDCVGGEDEERELCKAR-CNSEQFYCRKSHRCIAISKVCDKQYDCDMGDDETPEACRLHKPEEESDAEVQKDYTKTEKVDESSIPSGTQSVSYYSLSLTQCKTRCDTM-DRFVCAAFIY---NHNATFGIRCTLLAQKIADVRPNDAVGESVY----YEKKQSCNPDQKFQCKNTRCVDKAALCNGYDDCGDFSDEVGPSSCPDPK-PTFSVRLSGGRGNYEGRVEVKLFNEWGLMCDDGLNENLGKVVCNQLGFPMGYEKLSKNAKKEYGRGDGKFLMGAVACRGTEASLYDCPHSGWKNIVNCTINNVAGIVCLWDRACTDKEFRCSG--RCIPLSQVCDGTQQCTDGADE-ESCGTPTLALRNGDGPSSGFLEITRNGIAGAVCDDMFGDNEAKVVCAQLGIPGRFRLEPEGTFKTPGGTVMWLQDVKCSGSESSLFSCAIRNWGRSLCEIREAVNLRCGLPEPTRTTTQPPTTTTPARVTSSAKTCGQRIGRFPAGRIINGYPAIRGQFPWQVGIRLKVSETDTRQHCGGTIINDRWIVTAAHCFDYPKSYYAIRVGDLDNMVTEEFERQFDIDLVFIHPQYSGQPSYDYDITLIRIQPDSKGYIEFNAAVQPACLPEKTLQTTRKTVCHISGWGSVGDMQLPRNLMAANVPLIANARCNQLYPGRITDRMQCAGFEAGGIDSCQGDSGGPLVCPIDGVYTLLGATSWGEGCAMPGSPGVYANIRNLRDWIDE 1225
BLAST of Neurotrypsin vs. TrEMBL
Match: A0A267FXX8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004114g1 PE=3 SV=1) HSP 1 Score: 761.911 bits (1966), Expect = 0.000e+0 Identity = 447/1170 (38.21%), Postives = 674/1170 (57.61%), Query Frame = 2
Query: 242 PIRSVLD----GAPETPERLRIRSD-GKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLG--SEASIFECXXXXXXX-XXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSD-KPSLPLVTELPRISDPPGKGNGGC-ADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADE--KNCSII-----NETEILVKYDKFK----SKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFS-ECHLMKQLQSEIP--DSLKHINYPGDHYSLKTDCT--GKYKCSSGMCIALSKSCDGRNDCGDNSDEM---NCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRK-HNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIV-LSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGL-TCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDI-HWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL-VETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
P+ ++L G P +P++ R+R + NEGRVEV T WGL C DY+ +ANV C+ LGF A + + G G + + D +I +KC ++++F+CSH + ++GV C +N GC +GW +++G+CY+V + +T + A + CK + +KL + + EN+F++N+LY++F+ I WTGG YA+ + W ++ TIH V + S LWF G+ E SD RP+G+ +K+SC+A+++++ S ++ D NYFWWDN+SC T+LP+ICQK+ T +DCY +G YRG +RT++GT C WS L VN THP GLG+HN CRNPD D PWCWV+ + V+GYC + C + + L T+L P + C + +Q++CPSM +Y C+ +VCD DC G EDE E CK C + +FYC+ CI S +CD Q C DE + C + ++ E+ Y K + S I + Y +L L +C +C + +F C + Y N F C L+ Q +++ D++ Y Y K C K++C + C+ + C+G +DCGD SDE+ +CP P F +RL+G + ++EG+VE++ EWG +CDD + V+C QLGF E + + K D L+ + C+GTE S+ +CPH W+ NC +AG++C DRACT+ EFRC CI ++Q+CDG C+D +DE E ++ L NG SG +EI R+ G VCDD F D+EA V+C LGI GR + + F + G ++ L + +C G+E+SL C WG C +E + CGLP + T T+ + TCG++ GR++ G+ + G FPWQVG+R +V + D CGG+I+++ WI++A+HC++ PK+ Y +R GD +N + +E+ F+I+ +F++P+Y G P++D+D LI+I+ ++ I FN VQP CLP+ + KT C +SGWG ++ L+AA VPLI RC + Y RITDRMQCAG+EAGGID+CQGDSGGPLVC ++G+YT+ GATSWG+GCA+P SPGVYA + ++ D++ E
Sbjct: 85 PVDTLLKYRYGGVPVSPQKARLRGELSHMNPNEGRVEVEYRGT--WGLACGDYFGREEANVYCRNLGFKKGANKPYGRSFFGTGNFKRWDILIGHVKCAAYRGQSNLFQCSHNLMPACSLNNTAGVECTYNEGCEFGWSAYDGLCYRVESGPMTAEDAAKACKAKGAKLVAVNSQGENSFITNMLYTKFQDIAAAWTGGQYARFRDQLE--YDAWTFKDTIHLVK--SDSKLWFPGFTE--SDARPIGNKEKQSCIAISKIFYKSSERRDV-DTNYFWWDNISCKTELPYICQKLGTL-TSDCYSQSGGNYRGEAARTDKGTPCQPWSENRL-VNSMTHPGQGLGEHNLCRNPDGDTKPWCWVDNERNVFGYCSLRLCGEGEGGDGLPTDLK-----PSYNHSRCDSPDQYFCPSMINYRPTCVDRRQVCDSHKDCVGGEDEERELCKAR-CNSEQFYCRKSHRCIAISKVCDKQYDCDMGDDETPEACRLHKPEEESDAEVQKDYTKTEKVDESSIPSGTQSVSYYSLSLTQCKTRCDTM-DRFVCAAFIY---NHNATFGIRCTLLAQKIADVRPNDAVGESVY----YEKKQSCNPDQKFQCKNTRCVDKAALCNGYDDCGDFSDEVGPSSCPDPK-PTFSVRLSGGRGNYEGRVEVKLFNEWGLMCDDGLNENLGKVVCNQLGFPMGYEKLTKNAKKEYGRGDGKFLMGAVACRGTEASLYDCPHSGWKNIVNCTINNVAGIVCLWDRACTDKEFRCSG--RCIPLNQVCDGTQQCTDGADE-ESCGTPTLALRNGDGPSSGFLEITRNGIAGAVCDDMFGDNEAKVVCAQLGIPGRFRLEPEGTFKTPGGTVMWLQDVKCSGSESSLFSCAIRNWGRSLCEIREAVNLRCGLPEPTRTTTQPPTTTTPARVTSSAKTCGQRIGRFPAGRIINGYPAIRGQFPWQVGIRLKVSETDTRQHCGGTIINDRWIVTAAHCFDYPKSYYAIRVGDLDNMVTEEFERQFDIDLVFIHPQYSGQPSYDYDITLIRIQPDSKGYIEFNAAVQPACLPEKTLQTTRKTVCHISGWGSVGDMQLPRNLMAANVPLIANARCNQLYPGRITDRMQCAGFEAGGIDSCQGDSGGPLVCPIDGVYTLLGATSWGEGCAMPGSPGVYANIRNLRDWIDE 1225
BLAST of Neurotrypsin vs. TrEMBL
Match: A0A267GUN8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012109g1 PE=3 SV=1) HSP 1 Score: 746.888 bits (1927), Expect = 0.000e+0 Identity = 457/1245 (36.71%), Postives = 668/1245 (53.65%), Query Frame = 2
Query: 113 SENVLFHSHFQRHKRQA--PLGIEKTFFNDNPE----VVITEGRSKTIYPIRSVLDGAPETPERLRIRSD-GKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCL--GSEASIFECXXXXXXX-XXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQ-FYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHC--SDNADEKNC---------SIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDC--TGKYKCSSGMCIALSKSCDGRNDCGDNSDEMN--CPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHN-CKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIV-LSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRV-------GDYDI----HWCGGSILSEDWIISASHCY---EKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPG--MVPDLKTKCLVSGWGDT-LVETAEKLLAAEVPLIKAERCKEFYK-DRITDRMQCAGYEAGGIDTCQGDSGGPLVCQV----------------------NGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
+ L S R +R+A P + F + NPE T SK R G P TP+ +RIR D K NEGRVEV + WGLVC DY +A + C QLGF A + ++G G + ++D I+ ++C G E ++F+C+ ++ ++G+ CI+N GC +GW SF+G+CY++ + T +A C+ + +KL + E N+++N+LY++F+ ++ VWTGG + + + G W + TIH Q LWF G+ E +D+RP GD +++SC+A+++L+ + RS DVNYFWWDN+SC T+LPF+CQK+ DCYEG G YRG +RT++GT C W + L +N T+PNAGL H CRNPD D PWCWV+ V+GYC + +CSD+ LP E P ++ PG G+ C ++ F+CPSM SY C+ +VCD DC G EDE+ FC+ + C ++ FYC+ CI +CDG C D+ E+ C + NE ++ D +S + +Y L +C + C + QF C+S Y E R S+C L+ ++ P S + +Y + C + C++ C+ S++C+G +DCGD SDE+N C +E+ L+G ++ EG++EIR + G +CDD + A VIC+QLG+ ++ + +FG+ I L + C +I +C H W C + +AG+IC +DR C+ E RC D C +S+MCDG DCSD DE E L+V L + + +G VEI R +G VCDD F+D E V+CR LGI GR +F + G ++ L + RC G E SL C+ WGE CS E ++CGLP + TT A SE CG++ R+ GG + PWQV +R +V D + WCGG+ILS+ WI+SA+HC+ PK+ + VR GDWNN D YE+ F+++ L + +Y GPP +DFD +L++IK ++ + FN VQP CLP+ M D +KCL+SGWG + E L AEVPLI+ E C Y+ + IT+RM CAGY+ GGID CQGDSGGPLVC++ +T+ G TS+G+GCA P SPGVY V +++
Sbjct: 53 AAKTLEGSRHARSRREATSPFIPLRLFGDSNPEPQPGYPRTSPDSKPDELSRHRTGGVPPTPQIVRIRGDLMKMNPNEGRVEV--NYHGVWGLVCGDYVQQIEATLVCHQLGFELGANRVYKNSYYGTGHYKRQDIIVGHVQCKHDGDELTLFDCNRKLMPPCDLNSTTGIECIYNTGCDWGWSSFDGLCYRLETEPKTATEAATACQAKGAKLVSVNSLEEGNYITNMLYTKFRSLNAVWTGGQFGSFR-DVGSQYTSWTFVDTIHMARQ----PLWFPGF-EPGTDQRPAGDPQQRSCIAMSKLF-QNPRSRRDVDVNYFWWDNISCKTRLPFVCQKLAV-ASADCYEGIGTDYRGLAARTDKGTPCQPWGDNQL-INPSTYPNAGLSGHGLCRNPDGDAKPWCWVDNKNNVFGYCSLRQCSDE--LPQPQEGPPMT--PGFGHEKCDRHEDFFCPSMLSYRPVCVVERQVCDGFVDCVGKEDEDEAFCRNYTCTSDHFYCRKFHKCISMDQVCDGLADCDLGDDETEEACMSVKAQAEAELANEFQMTAGID--ESSVPADAKLAIYYRKTLVQCAKHCDRL-EQFFCKSFIYYRTGEQRFQSKCFLLGSRITD-PASKADPSIEAKYYEIPETCNDADDFLCNNSHCVKKSQACNGFDDCGDFSDEINSRCGKDPAKNYELVLSGGSDNHEGRIEIRLFNQRGLVCDDGLNAAMADVICQQLGYPGHERFILNAWQQFGRGTGGFI-LSGVHCGPGAKNITDCAHRPWGDSGACSVKNVAGLICLIDRPCSMHEVRC--DSRCFPLSKMCDGVPDCSDGWDETECD-ILNVTLSH--NRTAGVVEISRGGIQGVVCDDHFSDEEVKVVCRHLGIPGRGALAQRGQFSTPAGVVMWLQDIRCEGTEASLFDCSLRDWGESTCSIDEAVQLLCGLPEVTDPETTQAPQTDYSE-----ICGKRLAPYSFYRIGGGRNSGITEVPWQVAIRKKVIRPAGLRKDIKLPSSSAWCGGTILSDRWILSAAHCFPYANMPKSAFVVRAGDWNNTAPDQYEREFDVDLLIQHERYTGPPAYDFDISLLRIKPDSRGYLEFNAGVQPACLPRQNQPMNYDPTSKCLISGWGSRGGFDYPEALQMAEVPLIRREVCDRMYEFENITERMFCAGYKEGGIDACQGDSGGPLVCKIVDPGPPAEAGGAAAKGGPLRSGEARHTVVGITSFGNGCAEPNSPGVYTHVPYFRNWI 1267
BLAST of Neurotrypsin vs. TrEMBL
Match: A0A1I8IHJ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 743.806 bits (1919), Expect = 0.000e+0 Identity = 442/1170 (37.78%), Postives = 665/1170 (56.84%), Query Frame = 2
Query: 242 PIRSVLD----GAPETPERLRIRSD-GKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLG--SEASIFECXXXXXXX-XXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSD-KPSLPLVTELPRISDPPGKGNGGC-ADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADE--KNCSII-----NETEILVKYDKFK----SKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFS-ECHLMKQLQSEIP--DSLKHINYPGDHYSLKTDCT--GKYKCSSGMCIALSKSCDGRNDCGDNSDEM---NCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRK-HNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIV-LSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGL-TCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDI-HWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL-VETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
P+ ++L G P +P++ R+R + NEGRVEV T WGL C DY+ +ANV C+ LGF A + + G G + + D +I +KC ++++F+CSH + ++GV C +N GC +GW +++G+CY+V + +T + A + CK + +KL + + EN+F++N+LY++F+ I WTGG YA+ + W ++ TIH V + S LWF G+ E SD RP+G+ +K+SC+A+++++ S ++ D NYFWWDN+SC T+LP+ICQK+ T +DCY +G YRG +RT++GT C WS L VN THP GLG+HN CRNPD D PWCWV+ + V+GYC + C + + L T+L P + C + +Q++CPSM +Y C+ +VCD DC G EDE E C I S +CD Q C DE + C + ++ E+ Y K + S I + Y +L L +C +C + +F C + Y N F C L+ Q +++ D++ Y Y K C K++C + C+ + C+G +DCGD SDE+ +CP P F +RL+G + ++EG+VE++ EWG +CDD + V+C QLGF E + + K D L+ + C+GTE S+ +CPH W+ NC +AG++C DRACT+ EFRC CI +SQ+CDG C+D +DE E ++ L NG SG +EI R+ G VCDD F D+EA V+C LGI GR + + F + G ++ L + +C G+E+SL C WG C +E + CGLP + T T+ + TCG++ GR++ G+ + G FPWQVG+R +V + D CGG+I+++ WI++A+HC++ PK+ Y +R GD +N + +E+ F+I+ +F++P+Y G P++D+D LI+I+ ++ I FN VQP CLP+ + KT C +SGWG ++ L+AA VPLI RC + Y RITDRMQCAG+EAGGID+CQGDSGGPLVC ++G+YT+ GATSWG+GCA+P SPGVYA + ++ D++ E
Sbjct: 85 PVDTLLKYRYGGVPVSPQKARLRGELSHMNPNEGRVEVEYRGT--WGLACGDYFGREEANVYCRNLGFKKGANKPYGRSFFGTGNFKRWDILIGHVKCAAYRGQSNLFQCSHNLMPACSLNNTAGVECTYNEGCEFGWSAYDGLCYRVESGPMTAEDAAKACKAKGAKLVAVNSQGENSFITNMLYTKFQDIAAAWTGGQYARFRDQLE--YDAWTFKDTIHLVK--SDSKLWFPGFTE--SDARPIGNKEKQSCIAISKIFYKSSERRDV-DTNYFWWDNISCKTELPYICQKLGTL-TSDCYSQSGGNYRGEAARTDKGTPCQPWSENRL-VNSMTHPGQGLGEHNLCRNPDGDTKPWCWVDNERNVFGYCSLRLCGEGEGGDGLPTDLK-----PSYNHSRCDSPDQYFCPSMINYRPTCVDRRQVCDSHKDCVGGEDEERELC------------------IAISKVCDKQYDCDMGDDETPEACRLHKPEEESDAEVQKDYTKTEKVDESSIPSGTQSVSYYSLSLTQCKTRCDTM-DRFVCAAFIY---NHNATFGIRCTLLAQKIADVRPNDAVGESVY----YEKKQSCNPDQKFQCKNTRCVDKAALCNGYDDCGDFSDEVGPSSCPDPK-PTFSVRLSGGRGNYEGRVEVKLFNEWGLMCDDGLNENLGKVVCNQLGFPMGYEKLSKNAKKEYGRGDGKFLMGAVACRGTEASLYDCPHSGWKNIVNCTINNVAGIVCLWDRACTDKEFRCSG--RCIPLSQVCDGTQQCTDGADE-ESCGTPTLALRNGDGPSSGFLEITRNGIAGAVCDDMFGDNEAKVVCAQLGIPGRFRLEPEGTFKTPGGTVMWLQDVKCSGSESSLFSCAIRNWGRSLCEIREAVNLRCGLPEPTRTTTQPPTTTTPARVTSSAKTCGQRIGRFPAGRIINGYPAIRGQFPWQVGIRLKVSETDTRQHCGGTIINDRWIVTAAHCFDYPKSYYAIRVGDLDNMVTEEFERQFDIDLVFIHPQYSGQPSYDYDITLIRIQPDSKGYIEFNAAVQPACLPEKTLQTTRKTVCHISGWGSVGDMQLPRNLMAANVPLIANARCNQLYPGRITDRMQCAGFEAGGIDSCQGDSGGPLVCPIDGVYTLLGATSWGEGCAMPGSPGVYANIRNLRDWIDE 1208
BLAST of Neurotrypsin vs. TrEMBL
Match: A0A267FEP6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020209g1 PE=3 SV=1) HSP 1 Score: 718.768 bits (1854), Expect = 0.000e+0 Identity = 440/1188 (37.04%), Postives = 644/1188 (54.21%), Query Frame = 2
Query: 113 SENVLFHSHFQRHKRQ--APLGIEKTFFNDNPE----VVITEGRSKTIYPIRSVLDGAPETPERLRIRSD-GKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCL--GSEASIFECXXXXXXX-XXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQ-FYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHC--SDNADEKNC---------SIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDC--TGKYKCSSGMCIALSKSCDGRNDCGDNSDEMN--CPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHN-CKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIV-LSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRV-------GDYDI----HWCGGSILSEDWIISASHCY---EKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPG--MVPDLKTKCLVSGWGDT-LVETAEKLLAAEVPLIKAERCKEFYK-DRITDRMQCAGYEAGGIDTCQGDSGGPLVCQV 3538
+ L S R +R+ +P + F + NPE T S+ R G P TP+ +RIR D K NEGRVEV WGLVC DY +A + C QLGF A + ++G G + ++D I+ ++C G E ++F+C ++ ++G+ CI+N GC +GW SF+G+CY++ + T +A C+ + +KL + E N+++N+LY++F+ ++ VWTGG + + + G W + TIH Q LWF G+ E +D+RP GD +++SC+A+++L+ + RS DVNYFWWDN+SC T+LPF+CQK+ DCYEG G YRG +RT++GT C W + L +N T+PNAGL H CRNPD D PWCWV+ V+GYC + +CSD+ LP E P ++ PG G+ C ++ F+CPSM SY C+ +VCD DC G EDE+ FC+ + C ++ FYC+ CI +CDG C D+ E+ C + NE ++ D +S + +Y L +C + C + QF C+S Y E R S+C L+ ++ P S + +Y + C + C++ C+ S++C+G +DCGD SDE+N C +E+ L+G ++ EG++EIR + G +CDD + A VIC+QLG+ ++ + +FG+ I L + C +I +C H W C + +AG+IC +DR C+ E RC D C +S+MCDG DCSD DE E L+V L + + +G VEI R +G VCDD F+D E V+CR LGI GR +F + G ++ L + RC G E SL C+ WGE CS E ++CGLP + TT A SE CG++ R+ GG + PWQV +R +V D + WCGG+ILS+ WI+SA+HC+ PK+ + VR GDWNN D YE+ F+++ L + +Y GPP +DFD +L++IK ++ + FN VQP CLP+ M D +KCL+SGWG + E L AEVPLI+ E C Y+ + IT+RM CAGY+ GGID CQGDSGGPLVC++
Sbjct: 56 AAKTLEGSRHARSRRETTSPFIPLRLFGDSNPEPQPGYPRTSPDSEPDELSRHRTGGVPPTPQIVRIRGDLMKMNPNEGRVEVNYHGV--WGLVCGDYVQQIEATLVCHQLGFELGANRVYKNSYYGTGHYKRQDIIVGHVQCKHDGDELTLFDCKRKLMPPCDLNSTTGIECIYNTGCDWGWSSFDGLCYRLETEPKTAAEAAAACQAKGAKLVSVNSLEEGNYITNMLYTKFRSLNAVWTGGQFGSFR-DVGSQYTSWTFVDTIHMARQ----PLWFPGF-EPGTDQRPAGDPQQRSCIAMSKLF-QNPRSRRDVDVNYFWWDNISCKTRLPFVCQKLAV-ASADCYEGIGTEYRGLAARTDKGTPCQPWGDNQL-INPSTYPNAGLSGHGLCRNPDGDAKPWCWVDNKNNVFGYCSLRQCSDE--LPQPQEGPPMT--PGFGHEKCDRHEDFFCPSMLSYRPVCVVERQVCDGFVDCVGKEDEDEAFCRNYTCTSDHFYCRKFHKCISMDQVCDGLADCDLGDDETEEACMSVKAQAEAELANEFQMTAGID--ESSVPADAKLAIYYRKTLVQCAKHCDRL-EQFFCKSFIYYRTGEQRFQSKCFLLGSRITD-PASKADPSIEAKYYEIPETCNDADDFLCNNSHCVKKSQACNGFDDCGDFSDEINSRCGKDPAKNYELVLSGGSDNHEGRIEIRLFNQRGLVCDDGLNAAMADVICQQLGYPGHERFILNAWQQFGRGTGGFI-LSGVHCGPGAKNITDCAHRPWGDSGACSVKNVAGLICLIDRPCSMHEVRC--DSRCFPLSKMCDGVPDCSDGWDETECD-ILNVTLSH--NRTAGVVEISRGGIQGVVCDDHFSDEEVKVVCRHLGIPGRGALAQRGQFSTPAGVVMWLQDIRCEGTEASLFDCSLRDWGESTCSIDEAVQLLCGLPEVTDPETTQAPQTDYSE-----ICGKRLAPYSFYRIGGGRNSGITEVPWQVAIRKKVIRPAGLRKDIKLPSSSAWCGGTILSDRWILSAAHCFPYANMPKSAFVVRAGDWNNTAPDQYEREFDVDLLIQHERYTGPPAYDFDISLLRIKPDSRGYLEFNAGVQPACLPRQNQPMNYDPTSKCLISGWGSRGGFDYPEALQMAEVPLIRREVCDRMYEFENITERMFCAGYKEGGIDACQGDSGGPLVCKI 1213
BLAST of Neurotrypsin vs. Ensembl Cavefish
Match: si:ch211-51a6.2 (neurotrypsin-like [Source:NCBI gene;Acc:103031031]) HSP 1 Score: 305.834 bits (782), Expect = 2.179e-88 Identity = 200/551 (36.30%), Postives = 294/551 (53.36%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFE-TVYGAFPWQVGVRHRVGDYDIH-WCGGSILSEDWIISASHCYEK---PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGG--IDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
N D M V+ P+ +RL G+ WEG++EI + GEWG +CDD +T A V+C+QLG+ V G + +ILLDE+KC+G E S+ EC H VW +H+C E G+ RC++ V+ +D P L V+LV G GRVE+ + G+VCDDG+ D A V+CR LG G ++A + FG G G I L N RC G E+SL +C + H C H EDAGVIC ++ A + Q +CG +P TQRR R + G + ++ G +PWQV + R IH CG ++++ W+++A+HC+++ +RY +R GD++ E D +E++ E + L+ KY ++ D AL+++K +G + FN H CLP + C+++GWG T E + LL A +PL+ + +CK+ Y R T RM CAG + +D+CQGDSGGPLVCQ NG +T+ G SWG GC P PGVY +V L ++++ T
Sbjct: 321 NCDHMEDAGVSCNPYTDGVLRLVGADGPWEGRLEIYHSGEWGTVCDDNWTEHHAQVVCRQLGYSRGRAEVAPDGAY--EEGTGMILLDEVKCRGDERSLLECEHAVWGRHDCSHSEDVGI-------------RCER-------------AVETNDVPAVPPVAGPL-VRLVAGESRKEGRVEVFINGLWGSVCDDGWNDINAGVVCRQLGFVGMSKARSMAYFGEGQGPIHLDNVRCVGTESSLGECPADGPNTHDCRHSEDAGVICDY------VSQPADDSSMAMQ----SCGLRPNTQRRRRRIIGGDKSLRGDWPWQVSLWLRAQSKRIHPLCGATLINSCWVVTAAHCFKRFGTDPSRYILRLGDYHTEERDDFERTLSPERIELHRKYH-SQGWENDIALVRLKGTDGNCVSFNPHTNAACLPNQSAKKVKRPAACVITGWGVTDSEYSRTLLQAWLPLLPSGKCKKRYGSRFTSRMLCAGSLSNHRRVDSCQGDSGGPLVCQGTNGRWTLTGVISWGHGCGDPSYPGVYTRVSRFLKWIEKVT 831 HSP 2 Score: 160.614 bits (405), Expect = 8.909e-40 Identity = 96/253 (37.94%), Postives = 129/253 (50.99%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGIS-GRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G ++EG+VE+ + G+WG ICDD + +A V+C+QLG +A + H FGK P ILLD ++C G E S+EECPH W +HNC E AGV C + DG ++LV + GR+EI S GTVCDD +T+ A V+CR LG S GRA+ D + G G I+L +CRG+E SL++C WG H CSH ED G+ C
Sbjct: 233 LRLVGGLEEFEGRVEVYHNGKWGTICDDHWDDIDAEVVCRQLGLGGVPKAWTWSH-----FGKGSGP-ILLDGVQCTGNELSLEECPHMPWGQHNCDHMEDAGVSC----------------------NPYTDG-----------------VLRLVGADGPWEGRLEIYHSGEWGTVCDDNWTEHHAQVVCRQLGYSRGRAEVAPDGAYEEGTGMILLDEVKCRGDERSLLECEHAVWGRHDCSHSEDVGIRC 440 HSP 3 Score: 134.42 bits (337), Expect = 1.463e-31 Identity = 85/254 (33.46%), Postives = 121/254 (47.64%), Query Frame = 2
Query: 2090 RLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIE---ALEYVH---GSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
RL G +++ G++E+ G WG +CD T +A VIC+QLG E AL++ + GSG + + L C+G E S+ EC + +C AGV+C PEG ++LV G + + GRVE+ + GT+CDD + D +A V+CR LG+ G +A T + FG G G I+L +C GNE SL +C WG+H C H EDAGV C
Sbjct: 125 RLIGGQSNKSGRLEVYLNGRWGTVCDTHLTDRDASVICRQLGLGEIGTALQHSYFGPGSG---------LFHYERLGCRGNENSLLECKSRKFVSGDCNHGNEAGVVCAA------------------------------------PEGN-GTPLRLVGGLEEFEGRVEVYHNGKWGTICDDHWDDIDAEVVCRQLGLGGVPKAWTWSHFGKGSGPILLDGVQCTGNELSLEECPHMPWGQHNCDHMEDAGVSC 332 HSP 4 Score: 83.9593 bits (206), Expect = 7.489e-16 Identity = 43/104 (41.35%), Postives = 60/104 (57.69%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLP 2842
+ +L+ G + SGR+E+ + GTVCD TD +ASVICR LG+ A+ + FG G G CRGNE SL++C S K+ C+H +AGV+C P
Sbjct: 123 AARLIGGQSNKSGRLEVYLNGRWGTVCDTHLTDRDASVICRQLGLGEIGTALQHSYFGPGSGLFHYERLGCRGNENSLLECKSRKFVSGDCNHGNEAGVVCAAP 226 HSP 5 Score: 60.4622 bits (145), Expect = 1.020e-8 Identity = 41/102 (40.20%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 254 VLDGAPE---TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
V+ APE TP RL G EGRVEVY++ KWG +CDD+W+ DA V C+QLG + T H G+G ++D ++C G+E S+ EC
Sbjct: 221 VVCAAPEGNGTPLRLV----GGLEEFEGRVEVYHNG--KWGTICDDHWDDIDAEVVCRQLGLGGVPKAWT-WSHFGKG---SGPILLDGVQCTGNELSLEEC 312 HSP 6 Score: 57.3806 bits (137), Expect = 9.955e-8 Identity = 30/81 (37.04%), Postives = 43/81 (53.09%), Query Frame = 2
Query: 1100 ITTRGRTDCYEG--NGATYRGTTSRTNQGTKCAKWSN-PSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
+ + G C EG Y G+ S+T+ G C KW+ P + +P+ GLGDH+YCRNPD + PWC+ G G+
Sbjct: 37 VQSSGPLSCSEGFTELGYYNGSVSQTDSGFPCLKWTEFPDYM---QQYPDRGLGDHSYCRNPDREANPWCFFRQRSGAIGW 114 HSP 7 Score: 57.3806 bits (137), Expect = 1.048e-7 Identity = 33/86 (38.37%), Postives = 50/86 (58.14%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R + EGRVEV+ + WG VCDD WN +A V C+QLGFV ++ A + G G + +D ++C+G+E+S+ EC
Sbjct: 460 VRLVAGESRKEGRVEVFINGL--WGSVCDDGWNDINAGVVCRQLGFVGMSK-ARSMAYFGEG---QGPIHLDNVRCVGTESSLGEC 539 HSP 8 Score: 56.6102 bits (135), Expect = 1.589e-7 Identity = 31/86 (36.05%), Postives = 44/86 (51.16%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G EGR+E+Y+S +WG VCDD W A V C+QLG+ E + G ++D++KC G E S+ EC
Sbjct: 340 LRLVGADGPWEGRLEIYHSG--EWGTVCDDNWTEHHAQVVCRQLGYSRGRAEVAPDGAYEEGTGM---ILLDEVKCRGDERSLLEC 420
BLAST of Neurotrypsin vs. Ensembl Cavefish
Match: si:ch73-127m5.1 (si:ch73-127m5.1 [Source:ZFIN;Acc:ZDB-GENE-130531-25]) HSP 1 Score: 299.671 bits (766), Expect = 2.432e-86 Identity = 189/555 (34.05%), Postives = 289/555 (52.07%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICG-LPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGV---RHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLK-TKCLVSGWG-DTLVETAEKLLAAEVPLIKAERCKEFYKDRITDR-MQCAGYEAGG----IDTCQGDSGGPLVCQVN-GIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
N D M V+ P+ +RL N WEG++E+ + G+WG +CDD +T A V+C+QLGF E FG+ +ILLD+++C+G+E S+ EC H VW +H+C E G+ C E +KD ++LV+G GRVE+ + G+VCD G+ D A+V+CR LG +G ++A FG+G G I L+N RC G E+SL +C + H C H +DAGV+C +P E I T TCG + +RR R+VGG++++ G +PWQ + G + CG ++++ W+++A+HC+++ +RY V+ GD++ E D +E+ +E + ++ KY +++ D ALI++K G + FN H CLP PG + C+++GWG + LL A VPL+ +CK+ Y +R T M CAG + D+CQGDSGGPLVCQ G + + G SWG GC P PGVY++V L ++++ T
Sbjct: 297 NCDHMEDAGVSCNPYTDGVVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRGGAELAPAG--MFGEGAG-LILLDDVQCEGSESSLLECKHSVWGRHDCSHSEDVGIRCHRGGEGLREEGGEKKD------------------------------MELVSGESRKEGRVEVFLNGEWGSVCDSGWNDVNAAVVCRQLGFTGVSKARPMGYFGAGKGPIHLANVRCTGKESSLGECPAKGSDSHVCKHGQDAGVVCDYVPEPETDIAIVGTH----------TCGIRGGAERRSKRIVGGYKSLRGDWPWQASLWLKSQSKGSQPL--CGATLINSCWLLTAAHCFKRDASRYVVKLGDYHTEEQDDFERVLPLERVEVHRKYSN-ESWEHDVALIRLKGAEGKCVSFNPHTNAACLPAPGSKWGKRPASCVITGWGVSGKMYHPRTLLQAWVPLLPTWQCKKRYGERFTSHSMLCAGSMSSDHRHHADSCQGDSGGPLVCQGEAGRWVLTGVISWGHGCGDPSYPGVYSRVSRYLPWIEQVT 805 HSP 2 Score: 169.859 bits (429), Expect = 8.392e-43 Identity = 98/261 (37.55%), Postives = 135/261 (51.72%), Query Frame = 2
Query: 2060 PSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
P T PP +RL G + D+EG+VE+ + G WG ICDD++ +A V+C+QLG +A + H FG+ P I LD ++C G E S++ECPH W KHNC E AGV C + DG V+LV+G + + GR+E+ S GTVCDD +T+ A V+CR LG G A+ FG G G I+L + +C G+E+SL++C WG H CSH ED G+ C
Sbjct: 202 PEGTGPP--LRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAH-----FGQGSGP-IQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC----------------------NPYTDG-----------------VVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRGGAELAPAGMFGEGAGLILLDDVQCEGSESSLLECKHSVWGRHDCSHSEDVGIRC 415 HSP 3 Score: 124.405 bits (311), Expect = 1.891e-28 Identity = 75/249 (30.12%), Postives = 111/249 (44.58%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G + G++E+ G+WG +CD +T +A V+C+QLG + Y + + L C+G E S+ EC + +C AG++C PEG ++LV G + + GRVE+ G++CDD + D +A V+CR LG+ G +A + FG G G I L +C GNE SL +C W +H C H EDAGV C
Sbjct: 97 VRLVGGSSSKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGLGYGIPYTLNKQHSYFGPGSGLFHYARLGCRGNERSLLECRSRKFVTSDCSHGSEAGLVCS------------------------------------PPEGT-GPPLRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAHFGQGSGPIQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC 308 HSP 4 Score: 77.7962 bits (190), Expect = 5.744e-14 Identity = 43/107 (40.19%), Postives = 62/107 (57.94%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTR---FGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLP 2842
+V+LV G S SGR+E+ + G+VCD +TD +ASV+CR LG+ + + FG G G + CRGNE SL++C S K+ CSH +AG++C P
Sbjct: 96 AVRLVGGSSSKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGLGYGIPYTLNKQHSYFGPGSGLFHYARLGCRGNERSLLECRSRKFVTSDCSHGSEAGLVCSPP 202 HSP 5 Score: 57.7658 bits (138), Expect = 7.133e-8 Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
Y GT S+T+ G+ C KW+ V + +P GLG+H+YCRNPD + PWC+ G G+
Sbjct: 28 YNGTVSQTDSGSPCLKWTEFPDYV--QQYPGRGLGEHSYCRNPDRESNPWCFFRQSSGAIGW 87 HSP 6 Score: 56.6102 bits (135), Expect = 1.787e-7 Identity = 33/76 (43.42%), Postives = 42/76 (55.26%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGR+EVY+S WG VCDD W A V C+QLGF A E G G ++D ++C GSE+S+ EC
Sbjct: 326 EGRLEVYHSG--DWGTVCDDNWTEQHAQVVCRQLGFRGGA-ELAPAGMFGEG---AGLILLDDVQCEGSESSLLEC 395 HSP 7 Score: 54.299 bits (129), Expect = 8.393e-7 Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G EGRVEVY+ +WG +CDD W+ DA V C+QLG + +A H G+G + +D ++C G+E S+ EC
Sbjct: 209 LRLVGGEEDFEGRVEVYHDG--RWGSICDDQWDDMDAEVVCRQLG-LGGIPKAWSWAHFGQGSGPIQ---LDAVQCTGNELSLDEC 288
BLAST of Neurotrypsin vs. Ensembl Cavefish
Match: prss12 (serine protease 12 [Source:ZFIN;Acc:ZDB-GENE-090313-45]) HSP 1 Score: 292.352 bits (747), Expect = 7.982e-84 Identity = 183/565 (32.39%), Postives = 281/565 (49.73%), Query Frame = 2
Query: 2003 SKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHW-CGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQ------PVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAG--YEAGGIDTCQGDSGGPLVCQV-NGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
SKS G ++C D++++ +RL G +EG++E+ Y+G+WG +C+D ++ + V+C+QLG+ + + FG PI LLD++ C G E S+ C W +H+C V C R L V+LV G + GRVEI GTVCDDG+TD +A V+CR LG SG A+A FG G G I + N +C G E SL C +G H C H EDAGVIC ++ L + CG KP R+ R++GG ++ G +PWQ VR R D CG ++++ W+++++HC+++ N Y VR GD++ + E+ + ++ + ++P Y + D+D AL+ + R+ G D V+ PVCLP + VP C ++GWGDT ++ L AEV ++ +C+ Y T RM CAG + +D+C+GDSGGPLVC+ G + ++G TSWG C + +PGVY KV + L ++++ T
Sbjct: 294 SKSPWGEHNC-DHTEDAGVSCTPLTDGSLRLVGGAAGFEGRLEVHYRGQWGTVCNDGWSTTNTQVVCRQLGYSVSPDM-------FGASSGPI-LLDDVICTGKEPSLTHCSRSDWLRHDCPHSNNVAVACSPQRTGHGVPTS--------------------------------LPVRLVGGENEREGRVEISVGGQWGTVCDDGWTDQDAEVVCRQLGFSGLAKARVMAYFGEGEGPIHVDNVKCTGGERSLADCIKQPFGTHNCRHSEDAGVICNYGQQDN--------LAGVKDAVSSVCGLKPTHTRQKRIIGGENSLRGGWPWQAAVRLRGSQADGRLVCGATLINSCWVLTSAHCFKRYGNSTRMYKVRVGDYHTLVPEENEEEYGVDRIIIHPHYS-AQSNDYDLALVHLIRHGGPEGRGGDCVRQSRSVLPVCLPGRRERVPKGSANCFITGWGDTGRAYSKTLQQAEVRVLSKRQCERRYAGHFTSRMLCAGAALDDRRVDSCRGDSGGPLVCERGGGGWVLYGITSWGHSCRLQDAPGVYTKVSAFLPWIRKIT 808 HSP 2 Score: 140.198 bits (352), Expect = 1.954e-33 Identity = 85/260 (32.69%), Postives = 121/260 (46.54%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLD--ELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSI 2860
+RL G ++ +G VE+ G WG IC + + +A V+C+QLG + VR P+ ++ + C G E + +CPH VW C + + A V V F+ V+L G Y GRVE+ + GTVCDD + D++A V+CR LG+ G A+A FGSGVG ++L C GNE S+ QC+ WGEH C H EDAGV C P+ + S+
Sbjct: 109 VRLQGGRSKLDGTVEVYLNGVWGTICSNDWDDKDATVVCRQLG-----QGYKARARVSSLVRMPLSVVHWRTVGCSGEEGDLLQCPHSVWNGGECANRQAAAVTSAV-----------------------------------------FIPVRLSGGQSVYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGTARAWARAYFGSGVGRVLLDEVSCTGNELSIEQCSKSPWGEHNCDHTEDAGVSC-TPLTDGSL 321 HSP 3 Score: 138.272 bits (347), Expect = 8.888e-33 Identity = 92/282 (32.62%), Postives = 132/282 (46.81%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF-----IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTT 2869
N S F P +RL+G ++ +EG+VE+ + G+WG +CDD++ ++A V+C+QLG A Y GSGV G+V LLDE+ C G E SIE+C W +HNC E AGV C + + DG S++LV G + GR+E+ GTVC+DG++ + V+CR LG S V+ FG+ G I+L + C G E SL C+ W H C H + V C H + T+
Sbjct: 200 NRQAAAVTSAVFIP--VRLSGGQSVYEGRVEVYHAGQWGTVCDDQWDDNDAEVVCRQLGLGGTARAWARAYF-GSGV--GRV-----LLDEVSCTGNELSIEQCSKSPWGEHNCDHTEDAGVSC----------------------TPLTDG-----------------SLRLVGGAAGFEGRLEVHYRGQWGTVCNDGWSTTNTQVVCRQLGYS-----VSPDMFGASSGPILLDDVICTGKEPSLTHCSRSDWLRHDCPHSNNVAVACSPQRTGHGVPTS 427 HSP 4 Score: 61.2326 bits (147), Expect = 5.603e-9 Identity = 35/86 (40.70%), Postives = 49/86 (56.98%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EGRVEVY++ +WG VCDD W+ DA V C+QLG AR R + G G ++D++ C G+E SI +C
Sbjct: 214 VRLSGGQSVYEGRVEVYHAG--QWGTVCDDQWDDNDAEVVCRQLGLGGTARAWARA-YFGSG---VGRVLLDEVSCTGNELSIEQC 293 HSP 5 Score: 58.9214 bits (141), Expect = 2.943e-8 Identity = 29/71 (40.85%), Postives = 36/71 (50.70%), Query Frame = 2
Query: 1130 EGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGV--YGYC 1336
G YRG+ + T G C W+ S +P GLG HN+CRNPD I PWC+ T G +GYC
Sbjct: 34 RGRAGQYRGSIAVTESGASCMNWTEVSGV--SSRYPGKGLGHHNHCRNPDRRIRPWCFFRTGRGRIDWGYC 102 HSP 6 Score: 58.151 bits (139), Expect = 5.475e-8 Identity = 36/92 (39.13%), Postives = 47/92 (51.09%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P L +R G EGRVE+ S +WG VCDD W DA V C+QLGF A+ A + G G + +D +KC G E S+ +C
Sbjct: 424 VPTSLPVRLVGGENEREGRVEI--SVGGQWGTVCDDGWTDQDAEVVCRQLGFSGLAK-ARVMAYFGEG---EGPIHVDNVKCTGGERSLADC 509 HSP 7 Score: 50.8322 bits (120), Expect = 9.132e-6 Identity = 30/105 (28.57%), Postives = 51/105 (48.57%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPI 2845
SV+L G G VE+ + GT+C + + D +A+V+CR LG +A+A + + + C G E L+QC W +C++++ A V + I
Sbjct: 108 SVRLQGGRSKLDGTVEVYLNGVWGTICSNDWDDKDATVVCRQLGQGYKARARVSSLVRMPLSVVHWRTVGCSGEEGDLLQCPHSVWNGGECANRQAAAVTSAVFI 212
BLAST of Neurotrypsin vs. Ensembl Cavefish
Match: si:ch73-127m5.1 (si:ch73-127m5.1 [Source:ZFIN;Acc:ZDB-GENE-130531-25]) HSP 1 Score: 289.656 bits (740), Expect = 5.189e-83 Identity = 187/558 (33.51%), Postives = 287/558 (51.43%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICG-LPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGV---RHRVGDYDIHWCGGSILSEDWIISASHCYEKP---KNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLK-TKCLVSGWG-DTLVETAEKLLAAEVPLIKAERCKEFYKDRITDR-MQCAGYEAGG----IDTCQGDSGGPLVCQVN-GIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
N D M V+ P+ +RL N WEG++E+ + G+WG +CDD +T A V+C+QLGF G+G +ILLD+++C+G+E S+ EC H VW +H+C E D C++ C + V+LV+G GRVE+ + G+VCD G+ D A+V+CR LG +G ++A FG+G G I L+N RC G E+SL +C + H C H +DAGV+C +P E I T TCG + +RR R+VGG++++ G +PWQ + G + CG ++++ W+++A+HC+++ +RY V+ GD++ E D +E+ +E + ++ KY +++ D ALI++K G + FN H CLP PG + C+++GWG + LL A VPL+ +CK+ Y +R T M CAG + D+CQGDSGGPLVCQ G + + G SWG GC P PGVY++V L ++++ T
Sbjct: 286 NCDHMEDAGVSCNPYTDGVVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRYNFS-AEGAG---------LILLDDVQCEGSESSLLECKHSVWGRHDCSHSE--------DVVCSSQATHC-------------------------------VLVRLVSGESRKEGRVEVFLNGEWGSVCDSGWNDVNAAVVCRQLGFTGVSKARPMGYFGAGKGPIHLANVRCTGKESSLGECPAKGSDSHVCKHGQDAGVVCDYVPEPETDIAIVGTH----------TCGIRGGAERRSKRIVGGYKSLRGDWPWQASLWLKSQSKGSQPL--CGATLINSCWLLTAAHCFKRSGSDASRYVVKLGDYHTEEQDDFERVLPLERVEVHRKYSN-ESWEHDVALIRLKGAEGKCVSFNPHTNAACLPAPGSKWGKRPASCVITGWGVSGKMYHPRTLLQAWVPLLPTWQCKKRYGERFTSHSMLCAGSMSSDHRHHADSCQGDSGGPLVCQGEAGRWVLTGVISWGHGCGDPSYPGVYSRVSRYLPWIEQVT 781 HSP 2 Score: 149.828 bits (377), Expect = 1.791e-36 Identity = 94/258 (36.43%), Postives = 130/258 (50.39%), Query Frame = 2
Query: 2069 TFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
T PP +RL G + D+EG+VE+ + G WG ICDD++ +A V+C+QLG +A + H FG+ P I LD ++C G E S++ECPH W KHNC E AGV C + DG V+LV+G + + GR+E+ S GTVCDD +T+ A V+CR LG A G G I+L + +C G+E+SL++C WG H CSH ED V+C
Sbjct: 194 TGPP--LRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAH-----FGQGSGP-IQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC----------------------NPYTDG-----------------VVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRYNFSA-------EGAGLILLDDVQCEGSESSLLECKHSVWGRHDCSHSED--VVC 395 HSP 3 Score: 114.39 bits (285), Expect = 2.604e-25 Identity = 80/252 (31.75%), Postives = 115/252 (45.63%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVH---GSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+RL G + G++E+ G+WG +CD +T +A V+C+QLG AL++ + GSG+ F R L C+G E S+ EC R +K T SD + P ++LV G + + GRVE+ G++CDD + D +A V+CR LG+ G +A + FG G G I L +C GNE SL +C W +H C H EDAGV C
Sbjct: 107 VRLVGGSSSKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGLGYALQHSYFGPGSGL-FHYAR--------LGCRGNERSLLEC-------------------------------RSRKFVT--------------SDCTGPP-------LRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAHFGQGSGPIQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC 297 HSP 4 Score: 74.3294 bits (181), Expect = 6.082e-13 Identity = 46/126 (36.51%), Postives = 68/126 (53.97%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVIC 2395
GR+DC +S+++ C S +RL ++ EG+VE+ GEWG +CD + A V+C+QLGF + G FG + PI L + ++C G E S+ ECP H CK + AGV+C
Sbjct: 383 GRHDC-SHSEDVVCSSQATHCVLVRLVSGESRKEGRVEVFLNGEWGSVCDSGWNDVNAAVVCRQLGFT-GVSKARPMGY-FGAGKGPIHLAN-VRCTGKESSLGECPAKGSDSHVCKHGQDAGVVC 504 HSP 5 Score: 68.9366 bits (167), Expect = 3.069e-11 Identity = 38/92 (41.30%), Postives = 55/92 (59.78%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCS 2806
+V+LV G S SGR+E+ + G+VCD +TD +ASV+CR LG+ A+ + FG G G + CRGNE SL++C S K+ C+
Sbjct: 106 AVRLVGGSSSKSGRLEVYLNGQWGSVCDTHWTDRDASVVCRQLGL---GYALQHSYFGPGSGLFHYARLGCRGNERSLLECRSRKFVTSDCT 194 HSP 6 Score: 57.7658 bits (138), Expect = 6.870e-8 Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
Y GT S+T+ G+ C KW+ V + +P GLG+H+YCRNPD + PWC+ G G+
Sbjct: 38 YNGTVSQTDSGSPCLKWTEFPDYV--QQYPGRGLGEHSYCRNPDRESNPWCFFRQSSGAIGW 97 HSP 7 Score: 54.299 bits (129), Expect = 8.162e-7 Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G EGRVEVY+ +WG +CDD W+ DA V C+QLG + +A H G+G + +D ++C G+E S+ EC
Sbjct: 198 LRLVGGEEDFEGRVEVYHDG--RWGSICDDQWDDMDAEVVCRQLG-LGGIPKAWSWAHFGQGSGPIQ---LDAVQCTGNELSLDEC 277 HSP 8 Score: 52.373 bits (124), Expect = 3.049e-6 Identity = 31/76 (40.79%), Postives = 41/76 (53.95%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGR+EVY+S WG VCDD W A V C+QLGF R + G ++D ++C GSE+S+ EC
Sbjct: 315 EGRLEVYHSG--DWGTVCDDNWTEQHAQVVCRQLGF--------RYNFSAEG---AGLILLDDVQCEGSESSLLEC 377
BLAST of Neurotrypsin vs. Ensembl Cavefish
Match: si:ch73-127m5.1 (si:ch73-127m5.1 [Source:ZFIN;Acc:ZDB-GENE-130531-25]) HSP 1 Score: 286.574 bits (732), Expect = 2.542e-82 Identity = 186/558 (33.33%), Postives = 286/558 (51.25%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICG-LPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRG-RVVGGFETVYGAFPWQVGV---RHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLK-TKCLVSGWG-DTLVETAEKLLAAEVPLIKAERCKEFYKDRITDR-MQCAGYEAGG----IDTCQGDSGGPLVCQVN-GIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKT 3655
N D M V+ P+ +RL N WEG++E+ + G+WG +CDD +T A V+C+QLGF A + G G +ILLD+++C+G+E S+ EC H VW +H+C E G+ ++ T+ F +S G GRVE+ + G+VCD G+ D A+V+CR LG +G ++A FG+G G I L+N RC G E+SL +C + H C H +DAGV+C +P E I T TCG + +RR R+VGG++++ G +PWQ + G + CG ++++ W+++A+HC+++ +RY V+ GD++ E D +E+ +E + ++ KY +++ D ALI++K G + FN H CLP PG + C+++GWG + LL A VPL+ +CK+ Y +R T M CAG + D+CQGDSGGPLVCQ G + + G SWG GC P PGVY++V L ++++ T
Sbjct: 229 NCDHMEDAGVSCNPYTDGVVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRYNFSAGNCMFGEGAG-------LILLDDVQCEGSESSLLECKHSVWGRHDCSHSEDVGIPTYLN--LTSGWFWVLLGVLVRLVS----------------------------GESRKEGRVEVFLNGEWGSVCDSGWNDVNAAVVCRQLGFTGVSKARPMGYFGAGKGPIHLANVRCTGKESSLGECPAKGSDSHVCKHGQDAGVVCDYVPEPETDIAIVGTH----------TCGIRGGAERRSKRIVGGYKSLRGDWPWQASLWLKSQSKGSQPL--CGATLINSCWLLTAAHCFKRDASRYVVKLGDYHTEEQDDFERVLPLERVEVHRKYSN-ESWEHDVALIRLKGAEGKCVSFNPHTNAACLPAPGSKWGKRPASCVITGWGVSGKMYHPRTLLQAWVPLLPTWQCKKRYGERFTSHSMLCAGSMSSDHRHHADSCQGDSGGPLVCQGEAGRWVLTGVISWGHGCGDPSYPGVYSRVSRYLPWIEQVT 736 HSP 2 Score: 159.844 bits (403), Expect = 9.037e-40 Identity = 96/261 (36.78%), Postives = 134/261 (51.34%), Query Frame = 2
Query: 2054 NCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGV 2827
+C T PP +RL G + D+EG+VE+ + G WG ICDD++ +A V+C+QLG +A + H FG+ P I LD ++C G E S++ECPH W KHNC E AGV C + DG V+LV+G + + GR+E+ S GTVCDD +T+ A V+CR LG A + FG G G I+L + +C G+E+SL++C WG H CSH ED G+
Sbjct: 132 DCHQGTGPP--LRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAH-----FGQGSGP-IQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC----------------------NPYTDG-----------------VVRLVDGDNPWEGRLEVYHSGDWGTVCDDNWTEQHAQVVCRQLGFRYNFSA-GNCMFGEGAGLILLDDVQCEGSESSLLECKHSVWGRHDCSHSEDVGI 344 HSP 3 Score: 89.3521 bits (220), Expect = 1.145e-17 Identity = 43/100 (43.00%), Postives = 59/100 (59.00%), Query Frame = 2
Query: 2534 VKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
++LV G + + GRVE+ G++CDD + D +A V+CR LG+ G +A + FG G G I L +C GNE SL +C W +H C H EDAGV C
Sbjct: 141 LRLVGGEEDFEGRVEVYHDGRWGSICDDQWDDMDAEVVCRQLGLGGIPKAWSWAHFGQGSGPIQLDAVQCTGNELSLDECPHSGWEKHNCDHMEDAGVSC 240 HSP 4 Score: 57.3806 bits (137), Expect = 8.871e-8 Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
Y GT S+T+ G+ C KW+ V + +P GLG+H+YCRNPD + PWC+ G G+
Sbjct: 69 YNGTVSQTDSGSPCLKWTEFPDYV--QQYPGRGLGEHSYCRNPDRESNPWCFFRQSSGAIGW 128 HSP 5 Score: 54.299 bits (129), Expect = 7.581e-7 Identity = 33/86 (38.37%), Postives = 47/86 (54.65%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G EGRVEVY+ +WG +CDD W+ DA V C+QLG + +A H G+G +D ++C G+E S+ EC
Sbjct: 141 LRLVGGEEDFEGRVEVYHDG--RWGSICDDQWDDMDAEVVCRQLG-LGGIPKAWSWAHFGQG---SGPIQLDAVQCTGNELSLDEC 220 HSP 6 Score: 53.1434 bits (126), Expect = 1.687e-6 Identity = 31/76 (40.79%), Postives = 40/76 (52.63%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGR+EVY+S WG VCDD W A V C+QLGF G G ++D ++C GSE+S+ EC
Sbjct: 258 EGRLEVYHSG--DWGTVCDDNWTEQHAQVVCRQLGFRYNFSAGNCMFGEGAGL-----ILLDDVQCEGSESSLLEC 326
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005967.1 (pep scaffold:Pmarinus_7.0:GL493532:543:4961:1 gene:ENSPMAG00000005399.1 transcript:ENSPMAT00000005967.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 155.606 bits (392), Expect = 2.747e-39 Identity = 105/296 (35.47%), Postives = 144/296 (48.65%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKND-WEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVP 2902
G++DC + D SV IRL G +G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + I LD++ C G+E S+ CPH +W +HNC E AGVIC I S PE +++LV G GRVE+ S S GTVCDD + +A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA V+C S T TA L P
Sbjct: 394 GQHDCSHSED----ASVVCSETAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAP--SNAYFGQ-GNGTIWLDDVTCNGSEASLGNCPHLLWGQHNCVHAEDAGVICSA-----------------ITFS---------------PES----AIRLVGGGGPCQGRVEVLYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC-------SGTETAIRLVGGGGSP 639 HSP 2 Score: 154.836 bits (390), Expect = 4.784e-39 Identity = 98/273 (35.90%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKND-WEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
G++DC + D SV IRL G +G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + I LD++ C G+E S+ CPH W +HNC E AGVIC V + PE +++LV G GRVE+ S S GTVCDD + +A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA V+C
Sbjct: 180 GQHDCSHSED----ASVVCSETAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAP--SNAYFGQ-GNGTIWLDDVTCNGSEASLGNCPHLSWGQHNCVHAEDAGVICSV---------------------------------TFSPES----AIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC 408 HSP 3 Score: 143.665 bits (361), Expect = 2.308e-35 Identity = 83/239 (34.73%), Postives = 120/239 (50.21%), Query Frame = 2
Query: 2117 EGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+G+VE+ + G WG +CDD + +++A V+C+++G AL S FG+ + I LD++ C G+E S+ CPH W +HNC E AGVIC +++LV G GRVE+ S S GTVCDD + +A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA V+C
Sbjct: 1 QGRVEVLFNGSWGTVCDDYWDMNDAQVVCRKIGCGAALSAP--SNAYFGQ-GNGTIWLDDVICNGSESSLGNCPHLPWGQHNCVHAEDAGVICSES------------------------------------------AIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC 194 HSP 4 Score: 139.813 bits (351), Expect = 3.966e-34 Identity = 92/274 (33.58%), Postives = 128/274 (46.72%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPP-FEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDS-YSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
G+++C D SVTF P IRL G +G+VE+ Y G WG +CDD + I +A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A V+C +++LV G S GRVE+ + S GTVCDD + ++A V+CR +G A ++ FG G G I L + C G+E SL C WG+H C H EDAGVIC
Sbjct: 285 GQHNCVHAEDAGVICSVTFSPESAIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCSE------------------------------------------TAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAPSNAYFGQGNGTIWLDDVTCNGSEASLGNCPHLLWGQHNCVHAEDAGVIC 513 HSP 5 Score: 134.42 bits (337), Expect = 1.881e-32 Identity = 84/250 (33.60%), Postives = 117/250 (46.80%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDS-YSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G +G+VE+ Y G WG +CDD + I +A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A V+C +++LV G S GRVE+ + S GTVCDD + ++A V+CR +G A ++ FG G G I L + C G+E SL C WG+H C H EDAGVIC
Sbjct: 95 IRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCSE------------------------------------------TAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAPSNAYFGQGNGTIWLDDVTCNGSEASLGNCPHLSWGQHNCVHAEDAGVIC 299 HSP 6 Score: 105.531 bits (262), Expect = 2.435e-23 Identity = 73/231 (31.60%), Postives = 107/231 (46.32%), Query Frame = 2
Query: 2057 CPSVTFPP-FEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDS-YSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRC 2743
C ++TF P IRL G +G+VE+ Y G WG +CDD + I +A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A V+C + TE +++LV G S GRVE+ + S GTVCDD + ++A V+CR +G A ++ FG G G I L + C
Sbjct: 513 CSAITFSPESAIRLVGGGGPCQGRVEVLYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC------SGTE----------------------------------TAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAPSNAYFGQGNGTIWLDDVTC 700 HSP 7 Score: 56.225 bits (134), Expect = 4.245e-8 Identity = 35/92 (38.04%), Postives = 51/92 (55.43%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+PE IR G +GRVEV+ S + WG VCDD+W++ DA V C+ +G AA A + G+G T +D ++C GSE+ + C
Sbjct: 304 SPES-AIRLVGGGGPCQGRVEVFYSGS--WGTVCDDFWDIIDAQVVCRMVG-CGAALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 388 HSP 8 Score: 55.4546 bits (132), Expect = 7.201e-8 Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 245 IRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
I S + +PE+ RL G +GRVEV S + WG VCDD+W++ DA V C+ +G AA A + G+G T +D ++C GSE+ + C
Sbjct: 512 ICSAITFSPESAIRLV----GGGGPCQGRVEVLYSGS--WGTVCDDFWDIIDAQVVCRMVG-CGAALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 603 HSP 9 Score: 55.4546 bits (132), Expect = 8.114e-8 Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G +GRVEV+ S + WG VCDD+W++ DA V C+ +G AA A + G+G T +D ++C GSE+ + C
Sbjct: 95 IRLVGGGGPCQGRVEVFYSGS--WGTVCDDFWDIIDAQVVCRMVG-CGAALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 174 HSP 10 Score: 55.0694 bits (131), Expect = 9.871e-8 Identity = 36/93 (38.71%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET RL G + +GRVEV + + WG VCDD+W++ DA V C+++G AA A + G+G T +D + C GSEAS+ C
Sbjct: 196 ETAIRLV---GGGGSPCQGRVEVLYAGS--WGTVCDDFWDINDAQVVCRKVG-CGAALSAPSNAYFGQGNGT---IWLDDVTCNGSEASLGNC 279 HSP 11 Score: 55.0694 bits (131), Expect = 9.871e-8 Identity = 36/93 (38.71%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET RL G + +GRVEV + + WG VCDD+W++ DA V C+++G AA A + G+G T +D + C GSEAS+ C
Sbjct: 410 ETAIRLV---GGGGSPCQGRVEVLYAGS--WGTVCDDFWDINDAQVVCRKVG-CGAALSAPSNAYFGQGNGT---IWLDDVTCNGSEASLGNC 493 HSP 12 Score: 52.7582 bits (125), Expect = 5.472e-7 Identity = 31/76 (40.79%), Postives = 45/76 (59.21%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+GRVEV + + WG VCDDYW++ DA V C+++G AA A + G+G T +D + C GSE+S+ C
Sbjct: 1 QGRVEVLFNGS--WGTVCDDYWDMNDAQVVCRKIG-CGAALSAPSNAYFGQGNGT---IWLDDVICNGSESSLGNC 70 HSP 13 Score: 51.2174 bits (121), Expect = 1.457e-6 Identity = 34/97 (35.05%), Postives = 52/97 (53.61%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKND-WEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKC 2305
G++DC + D S T IRL G +G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + I LD++ C
Sbjct: 609 GQHDCSHSEDASVVCSGT--ETAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAP--SNAYFGQ-GNGTIWLDDVTC 700
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Match: tmprss3a (transmembrane serine protease 3a [Source:ZFIN;Acc:ZDB-GENE-070912-70]) HSP 1 Score: 148.288 bits (373), Expect = 8.758e-39 Identity = 85/251 (33.86%), Postives = 133/251 (52.99%), Query Frame = 2
Query: 2906 LTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVET---AEKLLAAEVPLIKAERCK--EFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
L CG + R+VGG + G++PWQV ++ H CGG++L+ W++SA+HC++ ++ WN ++ + I ++ N +Y D+D AL+K+ + ++ + P CLP + C ++GWG T V + L VP++ E C Y +IT RM CAG+ GG D CQGDSGGPLVCQ + + GATS+G+GCA+ PGVY++V + LD++
Sbjct: 111 LACGSS--IKSTSRIVGGQDAQLGSWPWQVSLQFN----GQHTCGGTLLTPTWVLSAAHCFQG-----YLGPDMWNVTDL-TSNLAGSISKIITNSQYVDNTNNDYDIALVKLSKPLS----MSNTIGPACLPNYQTDFQVGMNCWITGWGATKVSAGPASTTLQEVMVPILSTETCNAANSYNGQITSRMVCAGFMQGGQDACQGDSGGPLVCQTGTTWFVVGATSFGEGCALTNFPGVYSRVTAYLDWI 345
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009259.1 (pep scaffold:Pmarinus_7.0:GL478809:471847:475891:1 gene:ENSPMAG00000008373.1 transcript:ENSPMAT00000009259.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 141.739 bits (356), Expect = 4.581e-38 Identity = 86/228 (37.72%), Postives = 122/228 (53.51%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL--VETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKV 3622
+VGG+E + PWQV + + H+CGGS++S +W++SA+HCY+ +R VR G+ N + EQ + + +P+Y T D D LIK+ N + Q V P P C +SGWG+T V + + L+ + P++ C+ Y IT+ M C GY GG D+CQGDSGGP+VC NG + G SWG GCA+P PGVY KV
Sbjct: 21 HIVGGYECAAHSQPWQVSL-----NIGYHFCGGSLISSEWVVSAAHCYQT-ASRISVRIGEHNIFVTEGTEQRIQASKAIRHPQYSSA-TIDNDIMLIKLSSPAT----LNQYAQAV--PLPSSCVGTGVMCTISGWGETQTSVGSPDVLMCVQAPVLSDTSCRNSYPGDITNNMICLGYLEGGKDSCQGDSGGPVVC--NG--QLQGIVSWGRGCALPNYPGVYTKV 231
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006915.1 (pep scaffold:Pmarinus_7.0:GL476378:414701:424985:-1 gene:ENSPMAG00000006243.1 transcript:ENSPMAT00000006915.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 153.295 bits (386), Expect = 5.810e-38 Identity = 96/275 (34.91%), Postives = 135/275 (49.09%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFEIRLTGSKND-WEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGL 2839
G++DC + D SV IRL G +G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + II LD++ C G+E S+ CPH W +HNC E AGVIC +++LV G GRVE+ S S GTVCDD + +A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA V+C +
Sbjct: 644 GQHDCSHSED----ASVVCSETAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAP--SNAYFGQ-GNGIIWLDDVTCNGSEASLGNCPHLSWGQHNCVHAEDAGVICSES------------------------------------------AIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCSV 869 HSP 2 Score: 149.058 bits (375), Expect = 1.126e-36 Identity = 87/241 (36.10%), Postives = 123/241 (51.04%), Query Frame = 2
Query: 2117 EGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGL 2839
+G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + I LD++ C G+E S+ CPH W +HNC E AGVIC +++L+ G S GRVE+ S S GTVCDD + ++A V+CRM+G A ++T FG G+G I L + RC G+E L C+ WG+H CSH EDA VIC +
Sbjct: 146 QGRVEVLYNGSWGTVCDDYWNINDAQVVCRKVGCGAALSAQ--SYAYFGQ-GNGTIWLDDVTCNGSESSLGNCPHLPWGQHNCVHAEDAGVICSES------------------------------------------AIRLIGGGSSCQGRVEVLYSGSWGTVCDDFWDINDAQVVCRMVGCGVALSAPSNTYFGQGIGTIWLDDVRCNGSEYFLGPCSHRPWGQHDCSHSEDASVICSV 341 HSP 3 Score: 147.517 bits (371), Expect = 3.695e-36 Identity = 90/249 (36.14%), Postives = 126/249 (50.60%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G +G+VE+ Y G WG +CDD + I +A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A V+C V+ K I S PE +++LV G GRVE+ S S GTVCDD + +A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA V+C
Sbjct: 768 IRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCSVNGNVLLYIVYITKCSYTITFS---------------PES----AIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC 994 HSP 4 Score: 147.517 bits (371), Expect = 4.277e-36 Identity = 85/239 (35.56%), Postives = 122/239 (51.05%), Query Frame = 2
Query: 2117 EGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
+G+VE+ Y G WG +CDD + I++A V+C+++G AL S FG+ + I LD++ C G+E S+ CPH W +HNC E AGVIC +++L+ G S GRVE+ S S G+VCDD + ++A V+CRM+G A ++ FG G+G I L + RC G+E+ L C+ WG+H CSH EDA VIC
Sbjct: 358 QGRVEVLYNGSWGTVCDDYWNINDAQVVCRKVGCGAALSAQ--SYAYFGQ-GNGTIWLDDVTCNGSESSLGNCPHLPWGQHNCVHAEDAGVICSES------------------------------------------AIRLIGGGSSCQGRVEVLYSGSWGSVCDDFWDINDAQVVCRMVGCGVALSAPSNAYFGQGIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVIC 551 HSP 5 Score: 139.043 bits (349), Expect = 1.848e-33 Identity = 89/249 (35.74%), Postives = 121/249 (48.59%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G + +G+VE+ Y G WG +CDD + I++A V+C+ +G AL S FG+ I LD+++C G+E+ + C H W +H+C E A VIC V CT T R V GP GRVE+ + S GTVCDD + ++A V+CR +G A + FG G G I L + C G+E+SL C WG+H C H EDAGVIC
Sbjct: 240 IRLIGGGSSCQGRVEVLYSGSWGTVCDDFWDINDAQVVCRMVGCGVALSAP--SNTYFGQ-GIGTIWLDDVRCNGSEYFLGPCSHRPWGQHDCSHSEDASVICSV---CTETAIRL---------------------------------VGGGGGP--CQGRVEVLYNGSWGTVCDDYWNINDAQVVCRKVGCGAALSAQSYAYFGQGNGTIWLDDVTCNGSESSLGNCPHLPWGQHNCVHAEDAGVIC 447 HSP 6 Score: 134.42 bits (337), Expect = 3.990e-32 Identity = 84/250 (33.60%), Postives = 121/250 (48.40%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDS-YSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
IRL G + +G+VE+ Y G WG +CDD + I++A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A VIC +++LV G S GRVE+ + S GTVCDD + ++A V+CR +G A ++ FG G G I L + RC G+E+ L C+ WG+H CSH EDA V+C
Sbjct: 452 IRLIGGGSSCQGRVEVLYSGSWGSVCDDFWDINDAQVVCRMVGCGVALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVICSAAET----------------------------------------AIRLVGGGGSPCQGRVEVLFNGSWGTVCDDYWDMNDAQVVCRKIGCGAALSAPSNAYFGRGNGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVC 658 HSP 7 Score: 132.109 bits (331), Expect = 2.142e-31 Identity = 89/276 (32.25%), Postives = 130/276 (47.10%), Query Frame = 2
Query: 2018 GRNDCGDNSDEMNCPSVTFPPFE--IRLTGSKND-WEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDS-YSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
G++DC + D SV E IRL G +G+VE+ + G WG +CDD + +++A V+C+++G AL S FG+ + I LD+++C G+E + C H W +H+C E A V+C +++LV G S GRVE+ + S GTVCDD + ++A V+CR +G A ++ FG G G I L + C G+E SL C WG+H C H EDAGVIC
Sbjct: 537 GQHDCSHSED----ASVICSAAETAIRLVGGGGSPCQGRVEVLFNGSWGTVCDDYWDMNDAQVVCRKIGCGAALSAP--SNAYFGR-GNGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCSE------------------------------------------TAIRLVGGGGSPCQGRVEVLYAGSWGTVCDDFWDINDAQVVCRKVGCGAALSAPSNAYFGQGNGIIWLDDVTCNGSEASLGNCPHLSWGQHNCVHAEDAGVIC 763 HSP 8 Score: 129.798 bits (325), Expect = 1.317e-30 Identity = 85/253 (33.60%), Postives = 120/253 (47.43%), Query Frame = 2
Query: 2075 PPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
P IRL G + +G+VE+ Y G WG +CDD + I++A V+C+ +G AL S FG+ I LD+++C G+E + H W +H+C E A V+C +T I++ V GP GRVE+ + S GTVCDD + ++A V+CR +G A + FG G G I L + C G+E+SL C WG+H C H EDAGVIC
Sbjct: 25 PESAIRLIGGGSSCQGRVEVLYSGSWGTVCDDFWDINDAQVVCRMVGCGVALSAP--SNTYFGQ-GIGTIWLDDVRCNGSESFLGPYSHRPWGQHDCSHSEDASVVCS-------------ATETAIRL------------------------VGGGGGP--CQGRVEVLYNGSWGTVCDDYWNINDAQVVCRKVGCGAALSAQSYAYFGQGNGTIWLDDVTCNGSESSLGNCPHLPWGQHNCVHAEDAGVIC 235 HSP 9 Score: 91.2781 bits (225), Expect = 9.258e-19 Identity = 51/124 (41.13%), Postives = 70/124 (56.45%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVP 2902
+++L+ G S GRVE+ S S GTVCDD + ++A V+CRM+G A ++T FG G+G I L + RC G+E+ L + WG+H CSH EDA V+C S T TA L P
Sbjct: 28 AIRLIGGGSSCQGRVEVLYSGSWGTVCDDFWDINDAQVVCRMVGCGVALSAPSNTYFGQGIGTIWLDDVRCNGSESFLGPYSHRPWGQHDCSHSEDASVVC-------SATETAIRLVGGGGGP 144 HSP 10 Score: 78.5666 bits (192), Expect = 7.159e-15 Identity = 42/113 (37.17%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2063 SVTFPP-FEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICK 2398
++TF P IRL G +G+VE+ Y G WG +CDD + I +A V+C+ +G AL S FG+ I LD+++C G+E + C H W +H+C E A V+C
Sbjct: 886 TITFSPESAIRLVGGGGPCQGRVEVFYSGSWGTVCDDFWDIIDAQVVCRMVGCGAALSAP--SNAYFGQ-GIGTIWLDDVRCNGSESFLGPCSHRPWGQHDCSHSEDASVVCS 995 HSP 11 Score: 58.5362 bits (140), Expect = 8.504e-9 Identity = 39/100 (39.00%), Postives = 55/100 (55.00%), Query Frame = 2
Query: 251 SVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
SV+ A ET RL G + +GRVEV + + WG VCDDYW++ DA V C+++G AA A + GRG T +D ++C GSE+ + C
Sbjct: 548 SVICSAAETAIRLV---GGGGSPCQGRVEVLFNGS--WGTVCDDYWDMNDAQVVCRKIG-CGAALSAPSNAYFGRGNGT---IWLDDVRCNGSESFLGPC 638 HSP 12 Score: 56.225 bits (134), Expect = 4.836e-8 Identity = 35/92 (38.04%), Postives = 51/92 (55.43%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+PE IR G +GRVEV+ S + WG VCDD+W++ DA V C+ +G AA A + G+G T +D ++C GSE+ + C
Sbjct: 890 SPES-AIRLVGGGGPCQGRVEVFYSGS--WGTVCDDFWDIIDAQVVCRMVG-CGAALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 974 HSP 13 Score: 55.8398 bits (133), Expect = 7.082e-8 Identity = 41/101 (40.59%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 251 SVLDGAPETPERLRIRSDGKSAANEGRVEV-YNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
SV+ A ET RL +GRVEV YN WG VCDDYWN+ DA V C+++G AA A + G+G T +D + C GSE+S+ C
Sbjct: 125 SVVCSATETAIRLVGG---GGGPCQGRVEVLYNGS---WGTVCDDYWNINDAQVVCRKVG-CGAALSAQSYAYFGQGNGT---IWLDDVTCNGSESSLGNC 215 HSP 14 Score: 55.4546 bits (132), Expect = 8.904e-8 Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G +GRVEV+ S + WG VCDD+W++ DA V C+ +G AA A + G+G T +D ++C GSE+ + C
Sbjct: 768 IRLVGGGGPCQGRVEVFYSGS--WGTVCDDFWDIIDAQVVCRMVG-CGAALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 847 HSP 15 Score: 54.299 bits (129), Expect = 2.009e-7 Identity = 34/77 (44.16%), Postives = 45/77 (58.44%), Query Frame = 2
Query: 323 EGRVEV-YNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+GRVEV YN WG VCDDYWN+ DA V C+++G AA A + G+G T +D + C GSE+S+ C
Sbjct: 358 QGRVEVLYNGS---WGTVCDDYWNINDAQVVCRKVG-CGAALSAQSYAYFGQGNGT---IWLDDVTCNGSESSLGNC 427 HSP 16 Score: 53.1434 bits (126), Expect = 3.826e-7 Identity = 35/93 (37.63%), Postives = 50/93 (53.76%), Query Frame = 2
Query: 272 ETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
ET RL G + +GRVEV + + WG VCDD+W++ DA V C+++G AA A + G+G +D + C GSEAS+ C
Sbjct: 660 ETAIRLV---GGGGSPCQGRVEVLYAGS--WGTVCDDFWDINDAQVVCRKVG-CGAALSAPSNAYFGQG---NGIIWLDDVTCNGSEASLGNC 743 HSP 17 Score: 52.373 bits (124), Expect = 6.984e-7 Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G ++ +GRVEV S + WG VCDD+W++ DA V C+ +G A A + G+G T +D ++C GSE+ + C
Sbjct: 452 IRLIGGGSSCQGRVEVLYSGS--WGSVCDDFWDINDAQVVCRMVG-CGVALSAPSNAYFGQGIGT---IWLDDVRCNGSESFLGPC 531 HSP 18 Score: 52.373 bits (124), Expect = 7.731e-7 Identity = 33/87 (37.93%), Postives = 49/87 (56.32%), Query Frame = 2
Query: 275 TPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEA 535
+PE IR G ++ +GRVEV S + WG VCDD+W++ DA V C+ +G A A + G+G T +D ++C GSE+
Sbjct: 24 SPES-AIRLIGGGSSCQGRVEVLYSGS--WGTVCDDFWDINDAQVVCRMVG-CGVALSAPSNTYFGQGIGT---IWLDDVRCNGSES 103 HSP 19 Score: 51.6026 bits (122), Expect = 1.161e-6 Identity = 32/86 (37.21%), Postives = 47/86 (54.65%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
IR G ++ +GRVEV S + WG VCDD+W++ DA V C+ +G A A + G+G T +D ++C GSE + C
Sbjct: 240 IRLIGGGSSCQGRVEVLYSGS--WGTVCDDFWDINDAQVVCRMVG-CGVALSAPSNTYFGQGIGT---IWLDDVRCNGSEYFLGPC 319
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004829.1 (pep scaffold:Pmarinus_7.0:GL487651:3325:6603:-1 gene:ENSPMAG00000004392.1 transcript:ENSPMAT00000004829.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 141.739 bits (356), Expect = 5.938e-38 Identity = 85/228 (37.28%), Postives = 122/228 (53.51%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL--VETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKV 3622
+VGG+E + PWQV + + H+CGGS++S +W++SA+HCY+ +R VR G+ N + EQ + + +P+Y T D D LIK+ N + Q + P P C +SGWG+T V + + L+ + P++ C+ Y IT+ M C GY GG D+CQGDSGGP+VC NG + G SWG GCA+P PGVY KV
Sbjct: 21 HIVGGYECAAHSQPWQVSL-----NIGYHFCGGSLISSEWVVSAAHCYQT-ASRISVRIGEHNIFVTEGTEQRIQASKAIRHPQYNSA-TIDNDIMLIKLSSPAT----LNQYAQAI--PLPSSCVGTGVMCTISGWGETQTSVGSPDVLMCVQAPVLSDTSCRNSYPGDITNNMICLGYLEGGKDSCQGDSGGPVVC--NG--QLQGIVSWGRGCALPNYPGVYTKV 231
BLAST of Neurotrypsin vs. Ensembl Nematostella
Match: EDO31703 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX50]) HSP 1 Score: 174.866 bits (442), Expect = 1.044e-48 Identity = 108/277 (38.99%), Postives = 156/277 (56.32%), Query Frame = 2
Query: 2855 SITTTATSLKKSEQV--PG-LTCGRKPITQRRGRVVGGFETVYGAFPWQVGVR--HRVGDYDIHWCGGSILSEDWIISASHC--YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEK---LLAAEVPLIKAERCKEFYKD---RITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVK 3646
+ T T+L ++E+ PG + CG K + R+VGG GA+PWQ+ + H H CGGS+++ +WI++A+HC Y K Y + G+ + N D YEQ ++E + L+PKY D+D ALIK+ +ND V+PVCLP + T+C +SGWG L E L A VPL+ + C++ Y D +++ RM+CAGY AGGID CQGDSGGPLVC+ ++ + GA SWG GCA GVYA + + +V+
Sbjct: 16 TTTQAPTTLPRTERPIDPGSVKCGTK--GKGNTRIVGGTRAKKGAWPWQISMNYVHNKVTKTPHICGGSVVAPEWIVTAAHCFAYSKDAKDYTIAVGEHDLNATDGYEQRPDVERIILHPKYAPHNNHDYDVALIKLASPLQ----YNDRVRPVCLPSLKEDLEENTQCYISGWGH-LQEAGHGPWVLHQAAVPLVSRDTCQKAYNDLHYKVSSRMRCAGYGAGGIDACQGDSGGPLVCKEGDVWYLMGAISWGVGCARGGRYGVYADMMDLKYWVQ 285
BLAST of Neurotrypsin vs. Ensembl Nematostella
Match: EDO37083 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGX1]) HSP 1 Score: 171.014 bits (432), Expect = 2.978e-47 Identity = 99/258 (38.37%), Postives = 142/258 (55.04%), Query Frame = 2
Query: 2888 SEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYF-VRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVE-TAEKLLA-AEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
+ Q P +CG +P T R+VGG G +PWQ +R G +CGGS++ W+++A+HC + +R G N EQ ++E++ ++P Y+ P D ALIK+ + N HV VCLP P T+C ++GWG TA +L A VP++ RC++ Y +I D M CAG + GGIDTCQGDSGGP+VC+ G + I GATSWG GCA P GVYA V +++ +V+ +
Sbjct: 49 TTQAPVASCGVRPST----RIVGGTAAKQGDWPWQAQLRSTSG---FPFCGGSLIHPQWVLTATHCVSSRRPTDLNIRLGAHNRRANLGMEQDIKVEKIIMHPGYRKPVGLAHDIALIKLLKPAN----LNRHVNLVCLPDAVPAPTDGTRCWITGWGRLASGGTAPDILQQASVPVVSRARCEKAYPGKIHDSMLCAGLDQGGIDTCQGDSGGPMVCESRGRFYIHGATSWGYGCAQPGKFGVYAHVKNLVAWVRSE 295
BLAST of Neurotrypsin vs. Ensembl Nematostella
Match: EDO47761 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKX8]) HSP 1 Score: 166.007 bits (419), Expect = 3.180e-46 Identity = 100/244 (40.98%), Postives = 144/244 (59.02%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVG-DYDIHWCGGSILSEDWIISASHCYE--KPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWG---DTLVETAEKLLAAEVPLIKAERC--KEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVC---QVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
R+VGG GA+PWQV + G D +CGGS++ +W+++A+HC+E K K++Y +R G+ N NE + EQ F IE+ +++PKY T D D ALIK+ R N V +CLP+ TKC +SGWG + T++ L+ A+VPL+ ++C ++ Y DRIT+ M CAG GG+D+CQGDSGGP VC + +T+ G TSWG GCA G+YA V L ++
Sbjct: 1 RIVGGVVAKPGAWPWQVALIWAKGHDKGAQFCGGSLIDPEWVLTAAHCFEITKDKSQYMLRLGEHNFNEDEGTEQDFYIEKYYIHPKYD-EKTTDNDMALIKLDRPAT----LNKRVNTICLPEADDEFKPGTKCTISGWGALQEGAGSTSKVLMQAKVPLVSRDQCSHQQSYGDRITENMLCAGMRQGGVDSCQGDSGGPFVCTNPENPRQWTLVGVTSWGKGCARALKYGIYANVRRYLHWI 239
BLAST of Neurotrypsin vs. Ensembl Nematostella
Match: EDO34807 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNB8]) HSP 1 Score: 164.081 bits (414), Expect = 8.945e-46 Identity = 86/238 (36.13%), Postives = 136/238 (57.14%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKN--RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEK--LLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVK 3646
RV+GG + + GA+PWQ+ ++ R G++ CGGS++S W+++A+HC + N +Y + G+ N N ++ E++ ++++ +P+Y P D ALI++ + V PVCLP G ++C ++GWG + L A +P + +CK+ + + M CAG GGID CQGDSGGPLVC+ G + I GATSWG GCA P GVYA V +++ +V+
Sbjct: 2 RVIGGKDAIPGAWPWQIALKSR-GNFI---CGGSLVSSTWVVTAAHCVARSSNPAQYQIIVGEHNRNVNEVTEETLNVKKVIAHPQYNNP-RLSNDIALIELASPAK----LSSRVNPVCLPPHGYKLPAGSRCFITGWGKIKHPGSSHPILQQAMIPSLSEAQCKQKAQQSGCNSMLCAGLYNGGIDACQGDSGGPLVCETGGQFYIHGATSWGHGCAAPGKYGVYAHVKNLIGWVR 230
BLAST of Neurotrypsin vs. Ensembl Nematostella
Match: EDO34724 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ9]) HSP 1 Score: 162.54 bits (410), Expect = 4.761e-45 Identity = 91/237 (38.40%), Postives = 131/237 (55.27%), Query Frame = 2
Query: 2945 RVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEK-PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCL-PKPGMVPDLKTKCLVSGWGDTLVETAEK--LLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
R+VGG GA+PWQ +R+ G+ H+CGG+++ W+++ASHC K +VR G + EQ F I+ +F++P Y P + D AL+ + R + + CL P+ +PD T+C +SGWG A L+ A+VPL+ + C + Y R M CAG GG+D CQGDSGGP+VCQ G + + GATSWGDGCA GVYAK+ + D++
Sbjct: 2 RIVGGTTAQRGAWPWQAMLRYYNGN---HFCGGTLVHPRWVVTASHCVHKLTTGDLYVRMGAHWKDGKTGSEQDFRIQRIFMHPNYHSPVQYANDIALLLLDRPAR----LDRYTNLACLAPRSSPLPD-GTRCWISGWGRLSSGGASPNVLMQAQVPLVSGQTCSQAYPGRTHYSMLCAGLRHGGVDACQGDSGGPIVCQREGRWHLEGATSWGDGCAWANKYGVYAKITYLRDWL 230
BLAST of Neurotrypsin vs. Ensembl Medaka
Match: prss12 (serine protease 12 [Source:NCBI gene;Acc:105358156]) HSP 1 Score: 286.96 bits (733), Expect = 1.013e-81 Identity = 185/569 (32.51%), Postives = 286/569 (50.26%), Query Frame = 2
Query: 2003 SKSCDGRNDCGDNSDE-MNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGS-----GVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLT-CGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHW-CGGSILSEDWIISASHCYEKPKNR---YFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXX-----FNDHVQPVCLP-KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGG--IDTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
+KS G ++C + D ++C +T RL G EG++EI YQG+WG +CDD +T S + V+C+QLG+ + G +FG PI LLD++ C G E S+ C W H+C +E + C N E + ++ V+L G S GRVE+ GTVCDDG+TD +A V+CR LG SG A+A FG G G I + N C G+E S+ C G H C H EDAGVIC +HS K + P ++ CG + + R+ R++GG ++ G +PWQ +R R D CG +++ W+++++HC+++ N Y VR GD+++ + YE+ + ++++ +P+Y + D+D AL+ + F+ HV PVCLP + + + C ++GWGDT ++ L A + L+ C+++Y T RM CAG +D+C+GDSGGPLVC+ G + I+G TSWG C +SPGVY KV + ++++
Sbjct: 301 TKSAWGEHNCLHSEDAGVSCHPLT--DGATRLVGGAGSHEGRLEIFYQGQWGTVCDDGWTKSNSQVVCRQLGYRTGETLLLEGPDIPPGPRFGVGSGPI-LLDDVNCTGKEPSLLLCSRREWLCHDCSHQEDVHIAC-------NPEINGESLPASLR-------------------------VRLAGGESSREGRVELYLFGQWGTVCDDGWTDQDAEVVCRQLGYSGAAKARLMAYFGEGTGPIHMDNVNCIGDERSIADCIKQMPGTHNCRHSEDAGVICDYGEPQHST--------KEAKDPAISLCGLRLVHTRQRRIIGGENSLRGGWPWQAAIRLRGSKGDGRLVCGATLIDTCWVLTSAHCFKRYGNSTKLYKVRVGDYHSLVPEEYEEEYGVDKIVFHPRYH-SHSNDYDLALVHLSPGAVRQTAGECVSFSRHVLPVCLPARKERLLKQASNCHITGWGDTGRSYSKTLQQAPLSLLPRRLCQQYYHSAFTSRMLCAGSVQSDRRVDSCRGDSGGPLVCERPGGGWVIYGVTSWGHACRTQQSPGVYTKVSAFSFWIRK 825 HSP 2 Score: 140.584 bits (353), Expect = 2.085e-33 Identity = 93/275 (33.82%), Postives = 131/275 (47.64%), Query Frame = 2
Query: 2066 VTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTD-------TRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTT 2869
VT P +RL G +++ EG VE+ + G+WG ICDD++ S+A V+C+QLG A + FGK + LDE++C G E S+E+C W +HNC E AGV C + DG + +LV G S+ GR+EI GTVCDDG+T S + V+CR LG + + RFG G G I+L + C G E SL+ C+ +W H CSH+ED + C I S+ +
Sbjct: 217 VTLP---MRLIGGESESEGTVEVFHAGQWGSICDDQWDDSDAEVVCRQLGLSGAAKAW--GQAHFGKSSGR-VWLDEVRCTGNELSLEQCTKSAWGEHNCLHSEDAGVSCH----------------------PLTDG-----------------ATRLVGGAGSHEGRLEIFYQGQWGTVCDDGWTKSNSQVVCRQLGYRTGETLLLEGPDIPPGPRFGVGSGPILLDDVNCTGKEPSLLLCSRREWLCHDCSHQEDVHIACNPEINGESLPAS 446 HSP 3 Score: 124.405 bits (311), Expect = 1.908e-28 Identity = 82/267 (30.71%), Postives = 115/267 (43.07%), Query Frame = 2
Query: 2087 IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSG--VKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSL 2881
+RL G ++ +G+VE+ G WG +C + + +A V+C+QL E + G V + I E+ C+G E + C W C T C + KT + L ++L+ G G VE+ + G++CDD + DS+A V+CR LG+SG A+A FG G + L RC GNE SL QC WGEH C H EDAGV C H +T AT L
Sbjct: 113 VRLQGGRSKLDGRVEVYLGGVWGSVCSNDWGDEDAAVVCRQLD-----EGISGRARAVPLFRTSKTRIHWKEVHCRGEEPDLLHCSKSTWNGEEC---------------LTAAAVTCIQQKTVVT-----------------------LPMRLIGGESESEGTVEVFHAGQWGSICDDQWDDSDAEVVCRQLGLSGAAKAWGQAHFGKSSGRVWLDEVRCTGNELSLEQCTKSAWGEHNCLHSEDAGVSC------HPLTDGATRL 330 HSP 4 Score: 63.929 bits (154), Expect = 9.943e-10 Identity = 37/91 (40.66%), Postives = 50/91 (54.95%), Query Frame = 2
Query: 278 PERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
P LR+R G ++ EGRVE+Y +WG VCDD W DA V C+QLG+ AA+ A + G G +D + C+G E SI +C
Sbjct: 444 PASLRVRLAGGESSREGRVELY--LFGQWGTVCDDGWTDQDAEVVCRQLGYSGAAK-ARLMAYFGEG---TGPIHMDNVNCIGDERSIADC 528 HSP 5 Score: 62.003 bits (149), Expect = 4.150e-9 Identity = 34/96 (35.42%), Postives = 48/96 (50.00%), Query Frame = 2
Query: 1061 LSCNTKLPFICQKITTRGRTDCYEGNG--ATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGV--YGYC 1336
+S + LPF TDC G YRG+ T G++C W+ + + HP G+G+HN+CRNPD+ I PWC+ G +GYC
Sbjct: 23 VSSSVILPF----------TDCVRSRGKDGFYRGSIDVTESGSRCMNWTE--VAGFQERHPGKGIGEHNHCRNPDSRIRPWCFFRNHKGRIDWGYC 106 HSP 6 Score: 59.3066 bits (142), Expect = 2.604e-8 Identity = 33/88 (37.50%), Postives = 53/88 (60.23%), Query Frame = 2
Query: 287 LRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
L +R G + +EG VEV+++ +WG +CDD W+ DA V C+QLG AA+ A Q H G+ + +D+++C G+E S+ +C
Sbjct: 219 LPMRLIGGESESEGTVEVFHAG--QWGSICDDQWDDSDAEVVCRQLGLSGAAK-AWGQAHFGK---SSGRVWLDEVRCTGNELSLEQC 300 HSP 7 Score: 56.6102 bits (135), Expect = 1.619e-7 Identity = 42/123 (34.15%), Postives = 58/123 (47.15%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRML--GISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSE 2893
SV+L G GRVE+ G+VC + + D +A+V+CR L GISGRA+AV F + I CRG E L+ C+ W +C A V C I + ++ T L E
Sbjct: 112 SVRLQGGRSKLDGRVEVYLGGVWGSVCSNDWGDEDAAVVCRQLDEGISGRARAV--PLFRTSKTRIHWKEVHCRGEEPDLLHCSKSTWNGEEC--LTAAAVTC---IQQKTVVTLPMRLIGGE 227
BLAST of Neurotrypsin vs. Ensembl Medaka
Match: si:ch73-127m5.1 (neurotrypsin [Source:NCBI gene;Acc:101157816]) HSP 1 Score: 218.779 bits (556), Expect = 2.902e-58 Identity = 171/559 (30.59%), Postives = 262/559 (46.87%), Query Frame = 2
Query: 2039 NSDEMNCPSVTFPPFE---IRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYG--AFPWQVGV---RHRVGDYDIHWCGGSILSEDWIISASHCYEK---PKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLP-----KPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDR-MQCAGYEAGGI----DTCQGDSGGPLVCQ-VNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
N D M V+ P+ +RL G + WEG+VE+ + G+WG +CDD ++ A V+C+QLG+ E V + FGK +ILLD++ C+G+E S+ +C H +W + +C E GV C+V R ++ + + G + V+LV G GRVE+ G++CD G+ D A+V+CR LG SGRA A FG G G I L RC G E L +C S C +EDAGV C E ++ T K E+ L+CG + + + G+ E +PWQV V + G+ + C G+++S W+++++ C + Y VR G EQ+ E L L+ K+KG + D AL+K+ G + F+ + CLP K G +P + +GW + ++A+ VPL+ +CK+ + + + CA D CQG+SGG LVCQ +G + + G S G GCA P SP +Y +V ++ E
Sbjct: 319 NCDHMEDAGVSCSPYTDGVVRLVGGDSPWEGRVEVFHNGDWGTVCDDHWSQQHAEVVCRQLGYRGHAEVV--TDGLFGKGAG-LILLDDVHCEGSETSLLDCRHGIWGRTDCSHSEDVGVRCRV---------RSSQETNEVPVISPSTGPL----------------VRLVGGSSRKEGRVEVYLHGDWGSICDSGWNDLNAAVVCRQLGHSGRATAAVG--FGQGKGPIHLDQVRCTGKEEFLGECPSLGQSIQGCGRREDAGVKCDATPHEAAVKT------KPEE---LSCGLRKMVEVEGKKRNHEEESMSRTTWPWQVSVWLQSQKEGEGPL--CSGTLISPCWVLTSAFCLSRFGSDPTIYVVRVG--------ASEQTLTPERLVLHRKFKG-QSGGHDLALLKLPSTKGHCLTFDPNTNAACLPGTDGAKEGNIPASCVVMVTTGWAG-----PDSVVASWVPLMSPWQCKKRFGNSFSSHGTLCAASPPDTSLLRGDGCQGNSGGGLVCQDESGRWVLTGVVSGGYGCADPSSPALYTRVSRFRSWMDE 822 HSP 2 Score: 164.851 bits (416), Expect = 5.128e-41 Identity = 106/303 (34.98%), Postives = 147/303 (48.51%), Query Frame = 2
Query: 2060 PSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF---IEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGG 2959
P + PP +RL + D+EG+VE+ + G WG +CDD++ +A V+C+QLGF +A + H FG+ P ILLD +KC G E +++C H W +HNC E AGV C S DG V+LV G + GRVE+ + GTVCDD ++ A V+CR LG G A+ VTD FG G G I+L + C G+ETSL+ C WG CSH ED GV C S +++ +VP ++ P+ R+VGG
Sbjct: 224 PEGSGPP--LRLVEGEEDFEGRVEVFHAGRWGTVCDDQWDDRDAEVVCRQLGFGGIAKAWSWAH-----FGQGSGP-ILLDAVKCTGNELFLDQCSHGDWEQHNCDHMEDAGVSC----------------------SPYTDG-----------------VVRLVGGDSPWEGRVEVFHNGDWGTVCDDHWSQQHAEVVCRQLGYRGHAEVVTDGLFGKGAGLILLDDVHCEGSETSLLDCRHGIWGRTDCSHSEDVGVRC-----------RVRSSQETNEVPVISPSTGPLV----RLVGG 464 HSP 3 Score: 125.176 bits (313), Expect = 1.384e-28 Identity = 81/248 (32.66%), Postives = 116/248 (46.77%), Query Frame = 2
Query: 2090 RLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVIC 2833
RL GS G+VEI G+WG +C+ +T +A VIC+QLGF + + S +FG + + L C+G E ++ +C + +C A ++C PEG ++LV G + + GRVE+ + GTVCDD + D +A V+CR LG G A+A + FG G G I+L +C GNE L QC+ W +H C H EDAGV C
Sbjct: 123 RLVGSSISGSGRVEIYLNGQWGAVCNSHWTDRDASVICRQLGFGDIGTALQHS--QFGS-GSGLFHYERLGCRGDEGTLMKCQSRTFVTGDCSHGNEATLVCA------------------------------------PPEGS-GPPLRLVEGEEDFEGRVEVFHAGRWGTVCDDQWDDRDAEVVCRQLGFGGIAKAWSWAHFGQGSGPILLDAVKCTGNELFLDQCSHGDWEQHNCDHMEDAGVSC 330 HSP 4 Score: 74.3294 bits (181), Expect = 6.156e-13 Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = 2
Query: 2531 SVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLP 2842
+ +LV S SGRVEI + G VC+ +TD +ASVICR LG A+ ++FGSG G CRG+E +L++C S + CSH +A ++C P
Sbjct: 121 AARLVGSSISGSGRVEIYLNGQWGAVCNSHWTDRDASVICRQLGFGDIGTALQHSQFGSGSGLFHYERLGCRGDEGTLMKCQSRTFVTGDCSHGNEATLVCAPP 224 HSP 5 Score: 58.9214 bits (141), Expect = 3.620e-8 Identity = 27/63 (42.86%), Postives = 36/63 (57.14%), Query Frame = 2
Query: 1148 YRGTTSRTNQGTKCAKWSN-PSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGY 1333
Y GT S+T+ G C KW+ P + +P GLGDH+YCRNPD + PWC+ G G+
Sbjct: 53 YNGTVSQTDSGAPCLKWTEFPDYVMQ---YPGRGLGDHSYCRNPDRESNPWCFFRQSSGAIGW 112 HSP 6 Score: 57.7658 bits (138), Expect = 8.464e-8 Identity = 32/76 (42.11%), Postives = 46/76 (60.53%), Query Frame = 2
Query: 323 EGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
EGRVEV+++ +WG VCDD W+ DA V C+QLGF A+ A H G+G ++D +KC G+E + +C
Sbjct: 241 EGRVEVFHAG--RWGTVCDDQWDDRDAEVVCRQLGFGGIAK-AWSWAHFGQG---SGPILLDAVKCTGNELFLDQC 310 HSP 7 Score: 55.4546 bits (132), Expect = 3.584e-7 Identity = 32/86 (37.21%), Postives = 47/86 (54.65%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G + EGRVEV+++ WG VCDD+W+ A V C+QLG+ A E G+G ++D + C GSE S+ +C
Sbjct: 338 VRLVGGDSPWEGRVEVFHNG--DWGTVCDDHWSQQHAEVVCRQLGYRGHA-EVVTDGLFGKGAGL---ILLDDVHCEGSETSLLDC 417 HSP 8 Score: 50.8322 bits (120), Expect = 9.329e-6 Identity = 33/86 (38.37%), Postives = 44/86 (51.16%), Query Frame = 2
Query: 293 IRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFEC 550
+R G S+ EGRVEVY WG +CD WN +A V C+QLG A A G G+ K +D+++C G E + EC
Sbjct: 459 VRLVGGSSRKEGRVEVY--LHGDWGSICDSGWNDLNAAVVCRQLGHSGRATAAV-----GFGQ-GKGPIHLDQVRCTGKEEFLGEC 536
BLAST of Neurotrypsin vs. Ensembl Medaka
Match: tmprss3a (transmembrane serine protease 3 [Source:NCBI gene;Acc:101164532]) HSP 1 Score: 186.037 bits (471), Expect = 2.275e-50 Identity = 146/450 (32.44%), Postives = 210/450 (46.67%), Query Frame = 2
Query: 2315 EFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLV--ETAEKLLAAEVPLIKAERCK--EFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
+F DVW + S V+ V +C FRC + C+ S CDG DC ++ DE L ++G S +++QR TVC D + +S C+ LG S R SG +VL + R V C F + E +CS +G++ L LE CG +P Q R VVGG ++ G FPWQ + R G+ H CGGS++S WI++A+HC P + QS + ++ + +Y+ + D+D AL+++ + FN V+P+CLP G TKC +SGWG T E++ L AA VPLI C E Y I+ M CAGY GG+D+CQGDSGGPL C + + GATSWG+GCA PGVY +V + L +++++
Sbjct: 32 DFRFSSYQSDVWSSRDSISG-FRDVLLLVGLSCVG-RFRCGSSQKCVASSARCDGAADCPNQEDE------LGCVRLSGRRSV---LQVQRRGEWRTVCADNWNNSLGLSACKQLGYS---------RSYSGCSSLVLIHIR--------VLCCLF-YSESQCS----SGMVTTLKCLE--------------------CGSRPRYQTR--VVGGNLSLPGQFPWQASLHFR-GE---HMCGGSVISPLWIVTAAHC---PIH----------------AAQSLAVRKIVYHARYRSR-SLDYDIALMRLDQPLT----FNGLVEPICLPNHGEDFQEDTKCWISGWGATEEDGESSVVLRAAVVPLISTRTCNQPEVYAGHISSWMICAGYLEGGVDSCQGDSGGPLACHDPSAWKLVGATSWGEGCAARNRPGVYTRVTTALSWIRKQ 398 HSP 2 Score: 56.225 bits (134), Expect = 1.640e-7 Identity = 30/89 (33.71%), Postives = 48/89 (53.93%), Query Frame = 2
Query: 1955 DCTGKYKC-SSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGF 2218
C G+++C SS C+A S CDG DC + DE+ C +RL+G ++ ++++ +GEW +C D + S CKQLG+
Sbjct: 62 SCVGRFRCGSSQKCVASSARCDGAADCPNQEDELGC---------VRLSGRRS----VLQVQRRGEWRTVCADNWNNSLGLSACKQLGY 137
BLAST of Neurotrypsin vs. Ensembl Medaka
Match: tmprss5 (transmembrane serine protease 5 [Source:NCBI gene;Acc:101167385]) HSP 1 Score: 166.007 bits (419), Expect = 1.253e-45 Identity = 95/246 (38.62%), Postives = 136/246 (55.28%), Query Frame = 2
Query: 2927 ITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTL---VETAEKLLAAEVPLIKAERCKE--FYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKE 3649
IT + R++GG E G +PWQV + + H CGGSI++ WI++A+HC+ + + + F N+ Q +++E++ N Y T D D ALIK++ F D ++P+CLP+ T+C +SGWG T V++ + L A VPLI ++C Y IT RM CAGY G +D CQGDSGGPLVCQ + ++ + G SWG GCA P PGVY KV L ++ E
Sbjct: 16 ITTKLPRIIGGAEAALGRWPWQVSLYYS----SRHTCGGSIITRQWIVTAAHCHYRSEIQGF--------NQSLTAVQRWKLEKIIYNKNYNHR-THDSDIALIKLRTPLN----FTDTIRPICLPQYKYDLPGGTQCWISGWGYTKPDDVQSPDTLKEAPVPLISTKKCNSSCMYNGEITPRMLCAGYTEGKVDACQGDSGGPLVCQEDTVWRLVGVVSWGTGCAEPNHPGVYTKVAEFLSWIYE 244
BLAST of Neurotrypsin vs. Ensembl Medaka
Match: tmprss3a (transmembrane serine protease 3 [Source:NCBI gene;Acc:101164532]) HSP 1 Score: 165.236 bits (417), Expect = 3.717e-43 Identity = 128/419 (30.55%), Postives = 192/419 (45.82%), Query Frame = 2
Query: 2423 EFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHC------YEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLL-AAEVPLIKAERCK--EFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEK 3652
FRC + C+ S CDG DC ++ DE L ++G S +++QR TVC D + +S C+ LG S + V+ + P+ R C +G LP++ + ++ CG +P Q R VVGG ++ G FPWQ + R G+ H CGGS++S WI++A+HC + +P + +F+ + W ++ +Y + L D+D AL+++ + FN P+CLP G TKC +SGWG T + +L AA VPLI C E Y I+ M CAGY GG+D+CQGDSGGPL C + + GATSWG+GCA PGVY +V + L +++++
Sbjct: 68 RFRCGSSQKCVASSARCDGAADCPNQEDE------LGCVRLSGRRSV---LQVQRRGEWRTVCADNWNNSLGLSACKQLGYSRSSSLVSL--------DTTQPEPKTR-----------------TCLSVTASGC---LPVMTCPLPVSSE------------CGSRPRYQTR--VVGGNLSLPGQFPWQASLHFR-GE---HMCGGSVISPLWIVTAAHCDLKQCFHPRPPD-WFLASPLWRERKI-VYHARYRSRSL------------DYDIALMRLDQPLT----FNG--IPICLPNHGEDFQEDTKCWISGWGATEEDGDSVVLRAAVVPLISTRTCNQPEVYAGHISSWMICAGYLEGGVDSCQGDSGGPLACHDPSAWKLVGATSWGEGCAARNRPGVYTRVTTALSWIRKQ 411 HSP 2 Score: 57.3806 bits (137), Expect = 7.096e-8 Identity = 31/96 (32.29%), Postives = 50/96 (52.08%), Query Frame = 2
Query: 1955 DCTGKYKC-SSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYV 2239
C G+++C SS C+A S CDG DC + DE+ C +RL+G ++ ++++ +GEW +C D + S CKQLG+ + V
Sbjct: 64 SCVGRFRCGSSQKCVASSARCDGAADCPNQEDELGC---------VRLSGRRS----VLQVQRRGEWRTVCADNWNNSLGLSACKQLGYSRSSSLV 146
BLAST of Neurotrypsin vs. Planmine SMEST
Match: SMESG000016089.1 (SMESG000016089.1) HSP 1 Score: 2440.61 bits (6324), Expect = 0.000e+0 Identity = 1204/1206 (99.83%), Postives = 1205/1206 (99.92%), Query Frame = 2
Query: 44 MMHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSKTIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECXXXXXXXXXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
MMHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSK+IYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECSHEMSSTCSGGSSGVSCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEH ITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALIKIKRNNGIGIIFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF
Sbjct: 1 MMHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSKSIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECSHEMSSTCSGGSSGVSCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHPITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALIKIKRNNGIGIIFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 1206
BLAST of Neurotrypsin vs. Planmine SMEST
Match: SMESG000016089.1 (SMESG000016089.1) HSP 1 Score: 2438.3 bits (6318), Expect = 0.000e+0 Identity = 1203/1205 (99.83%), Postives = 1204/1205 (99.92%), Query Frame = 2
Query: 47 MHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSKTIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECXXXXXXXXXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
MHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSK+IYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECSHEMSSTCSGGSSGVSCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEH ITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALIKIKRNNGIGIIFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF
Sbjct: 1 MHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSKSIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECSHEMSSTCSGGSSGVSCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTGKYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHPITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALIKIKRNNGIGIIFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 1205
BLAST of Neurotrypsin vs. Planmine SMEST
Match: SMESG000016088.1 (SMESG000016088.1) HSP 1 Score: 1508.04 bits (3903), Expect = 0.000e+0 Identity = 727/1207 (60.23%), Postives = 914/1207 (75.72%), Query Frame = 2
Query: 56 ILIIWFCACANTQAF----QLENSENVLFHSHFQRHKRQAPLGIEKTFFNDNPEVVITEGRSKTIYPIRSVLDGAPETPERLRIRSDGKSAANEGRVEVYNSKTKKWGLVCDDYWNLPDANVSCKQLGFVNAAREATRQDHHGRGKWTKKDYIIDKIKCLGSEASIFECXXXXXXXXXXXXXXXXCIFNGGCSYGWVSFNGVCYQVVNKILTFKKAEEYCKNQNSKLAYILDRNENNFLSNVLYSQFKKIHQVWTGGYYAKIKMNTGKIVKRWNWQSTIHNVDQWNTSYLWFQGYGEIKSDKRPLGDVKKKSCLALNRLYGDSQRSNEIYDVNYFWWDNLSCNTKLPFICQKITTRGRTDCYEGNGATYRGTTSRTNQGTKCAKWSNPSLTVNDKTHPNAGLGDHNYCRNPDNDIMPWCWVNTDVGVYGYCVMEKCSDKPSLPLVTELPRISDPPGKGNGGCADNQFYCPSMTSYNRKCLALSRVCDQVNDCSGLEDENPEFCKTFFCQANEFYCKADTFCILKSNICDGQRHCSDNADEKNCSIINETEILVKYDKFKSKILMVPDKIVYANLRLEECVQKCRVIYPQFGCRSLNYLPPNETRIFSECHLMKQLQSEIPDSLKHINYPGDHYSLKTDCTG-KYKCSSGMCIALSKSCDGRNDCGDNSDEMNCPSVTFPPFEIRLTGSKNDWEGKVEIRYQGEWGYICDDRFTISEAHVICKQLGFIEALEYVHGSGVKFGKVRDPIILLDELKCKGTEFSIEECPHDVWRKHNCKSEEIAGVICKVDRACTNTEFRCQKDKTCIKMSQMCDGKVDCSDRSDEPEGQCFLSVKLVNGPDSYSGRVEIQRSLSKGTVCDDGFTDSEASVICRMLGISGRAQAVTDTRFGSGVGEIVLSNPRCRGNETSLVQCNSFKWGEHKCSHKEDAGVICGLPILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLAAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTGF 3661
+ ++ FC T A Q E +N F + RHKRQA E TFF+ N + GRS T +P+R V DGAPE+P+ +R+R + ANEGRVEVY+S +K WGLVCDDYWN+ DANV+C+QLGF AREATRQD +GRG W+ DY + KI+C G+E S+ +C H+ +S CSG S+GVSC++N GC GW+SFNG CYQ+ + + +K+A+++C++ NSKLA+IL ENNF+SNVLY+Q+K I Q+WTGGYYA++K++ G+ RWNW STIHNVDQW+TS LWF G+ E K+DKRP GD K +SC+ALNR + DSQR N +YDVNYFWWDNLSCN KLPFICQ + R+DCYE NG YRG SRT QG+ CAKW++P+ TVN +T+P AGLGDHN+CRNPDND+ PWCW N GVYGYC + KCS+ P P T P+++DP G G GGC Q++CPSM SYNR+C+ + VCD++ C G EDE+ FCK + C+ NEF+C +D FCILK+++CDG+R+C+D +DEKNC++++E+ +L YDK SKIL V DK+ Y ++ +++C +KC++++ QF C+S NYLPP+E+R SEC L+ +L SE P L I YPG HYS+K+DCT ++KC+ GMCI LSK CD R+DCGDNSDE +C +VTFPPFEIRLTGS N +EGKVE+RYQGEWG++CDD F I A V+CKQLGF+ A+E +H + +FGK R P +L+DE+KC GTE S+EEC H WR HNC+ +EIAGV+CKVDRACTN+EFRC KDK CI +S++CD DCSD SDEPEG+C + V L +GPD+ SGRVE+ R KGTVCDD + D +A+VICRML I GRAQA+ D RFG G G IVL N +C G E +L C FKWGEHKC+HKED GVICGLP + +T K + GL CG +PI +RGRVVGGFE VYGAFPWQVGVR +V DYD HWCG SILSEDWII A+HCY +PK+ Y VRTGDW+N D YEQ FEIEEL L+P + +D+D ALIKI+RN+G+GI FN++VQPVCLPK GMVPD +KCLVSGWGDTL EKLLAAEVPLIK ERC EFYK R+T+RMQCAGYEAGGIDTCQGDSGGPLVC+VNG+YTIFGATSWG+GCA P++PGVYAK+ LD++KE+TG
Sbjct: 3 LAVLAFCLVLTTNAAIIKSQYEKYDN-FFQASLTRHKRQAVSEDETTFFDQNIPPLTGTGRSNTRFPMRKVRDGAPESPQIIRLRGENALTANEGRVEVYDSNSKLWGLVCDDYWNILDANVTCRQLGFRRKAREATRQDRYGRGDWSVNDYTVHKIQCTGNEKSLVDCPHDSTSYCSGKSAGVSCVYNLGCPNGWISFNGACYQINTQQMDYKQADKFCQDHNSKLAFILKAKENNFISNVLYTQYKSIAQIWTGGYYAEVKLDEGRSEFRWNWYSTIHNVDQWSTSNLWFPGFYETKTDKRPQGDPKTQSCVALNRAFADSQRQNTVYDVNYFWWDNLSCNEKLPFICQMNIPQSRSDCYEDNGLDYRGRISRTVQGSSCAKWTDPAATVNPQTNPEAGLGDHNFCRNPDNDLQPWCWTNPTNGVYGYCAIGKCSELPP-PTATLKPQVTDPMGFGQGGCQSIQYHCPSMISYNRQCIPKNEVCDKIPHCLGKEDEDHGFCKKYLCKENEFFCASDMFCILKTHVCDGKRNCADGSDEKNCTVLDESALLKVYDKTDSKILDVSDKLDYIDIPIDKCAEKCKILFHQFACKSFNYLPPHESRTHSECRLLTKLASEEPYKLIAITYPGAHYSMKSDCTAEQFKCTRGMCIELSKKCDHRDDCGDNSDETDCTTVTFPPFEIRLTGSVNKYEGKVEVRYQGEWGFVCDDGFDIKAAEVVCKQLGFLGAMESIHSAVNRFGKNRSPNLLVDEIKCTGTENSLEECKHSPWRSHNCRPDEIAGVVCKVDRACTNSEFRCSKDKMCISLSKVCDKTNDCSDGSDEPEGKCTVKVTLADGPDAKSGRVELSRGGLKGTVCDDDYDDKDAAVICRMLQIPGRAQAIKDKRFGEGAGAIVLDNLKCTGEELNLENCPFFKWGEHKCTHKEDIGVICGLP--DEVVTQPPKPTAKVDPAAGLKCGHRPIVHKRGRVVGGFEAVYGAFPWQVGVRQKVDDYDSHWCGASILSEDWIIGAAHCYARPKSDYRVRTGDWDNASDDKYEQVFEIEELILHPNWNN-ANYDYDYALIKIERNDGVGIKFNNYVQPVCLPKLGMVPDPNSKCLVSGWGDTLSTYPEKLLAAEVPLIKHERCFEFYKGRLTERMQCAGYEAGGIDTCQGDSGGPLVCKVNGVYTIFGATSWGNGCAQPKNPGVYAKIQMQLDWIKEQTGL 1204
BLAST of Neurotrypsin vs. Planmine SMEST
Match: SMESG000002831.1 (SMESG000002831.1) HSP 1 Score: 165.622 bits (418), Expect = 8.267e-44 Identity = 97/271 (35.79%), Postives = 144/271 (53.14%), Query Frame = 2
Query: 2840 PILEHSITTTATSLKKSEQVPGLTCGRKPITQRRGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYE--KPKNRYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLL-AAEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFV 3643
P + + IT T ++S + C + I R+VGG G++PWQV +R+ G CGGSI++ WI++A+HC E + V G + N D Q +E++ N Y+ + D A++K+ ND+V+PVC+ VP +C ++GWG T + L A+VP+I C+ +Y + ++D M CAGYE GG D+CQGDSGGPLVC++N + + G TSWG GCA PR PGVY L ++
Sbjct: 161 PEILYQITPPETG-ERSNETSRAGCSYQSIPNTEMRIVGGIVAREGSWPWQVSLRNSDGVL----CGGSIVNSKWIVTAAHCVEGRSSPQTWTVHAGRFTLNGNDRNLQVNRVEKVIKNENYRSENN-ENDIAMMKLSGILR----LNDYVEPVCIGTS--VPRDNAECYITGWGVTHGTGGDGYLKQAKVPIIDQNTCQSWYGNSLSDGMICAGYENGGTDSCQGDSGGPLVCRINSKFQLIGITSWGMGCAEPRQPGVYTAASKYLQWM 419
BLAST of Neurotrypsin vs. Planmine SMEST
Match: SMESG000004375.1 (SMESG000004375.1) HSP 1 Score: 159.844 bits (403), Expect = 1.235e-39 Identity = 91/250 (36.40%), Postives = 142/250 (56.80%), Query Frame = 2
Query: 2939 RGRVVGGFETVYGAFPWQVGVRHRVGDYDIHWCGGSILSEDWIISASHCYEKPKN---------RYFVRTGDWNNNEMDIYEQSFEIEELFLNPKYKGPPTFDFDTALXXXXXXXXXXXXFNDHVQPVCLPKPGMVPDLKTKCLVSGWGDTLVETAEKLLA-AEVPLIKAERCKEFYKDRITDRMQCAGYEAGGIDTCQGDSGGPLVCQVNGIYTIFGATSWGDGCAVPRSPGVYAKVDSMLDFVKEKTG 3658
++VGG E G++PW + +++ +H CGGS+++ED++++A+HC+ N RYF+ G N +E++FL+P YK T DFD A++K+KR + +Q VC+P+ P KC++ GWG T E +L A VP+++ C E Y + IT++M CAGY+ GGID+C GDSGGPLVC+ + I+G S+G GCA+P PGVY + +D++ + G
Sbjct: 270 HSQIVGGSEANPGSWPWMISLKYG----PLHMCGGSLIAEDFVLTAAHCFIMDSNPDYYNIDLGRYFLYKGLVEN---------IAVEKIFLHPSYKIE-TQDFDLAIVKLKRKAKLTAS----IQTVCIPR-DKEPIAGKKCVILGWGSTQGTGGEGVLKQAVVPIVERNVCNELYGNIITNQMLCAGYKTGGIDSCSGDSGGPLVCKTEKKFEIYGIVSFGQGCALPNRPGVYTNIVRFVDWIDKIIG 500 The following BLAST results are available for this feature:
BLAST of Neurotrypsin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Neurotrypsin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Neurotrypsin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Neurotrypsin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Neurotrypsin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Neurotrypsin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005872 ID=SMED30005872|Name=Neurotrypsin|organism=Schmidtea mediterranea sexual|type=transcript|length=3682bpback to top protein sequence of SMED30005872-orf-1 >SMED30005872-orf-1 ID=SMED30005872-orf-1|Name=SMED30005872-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1207bp MMHVILIIWFCACANTQAFQLENSENVLFHSHFQRHKRQAPLGIEKTFFNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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