DUF3421 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005863 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16
Homology
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0345371) HSP 1 Score: 102.064 bits (253), Expect = 1.785e-25 Identity = 59/145 (40.69%), Postives = 87/145 (60.00%), Query Frame = 1 Query: 301 SWVKAN-NGSIPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGAL-VGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +W+ N GS+PP A+ AG + FV RA H+G+ +P K V G + +V +GG E +K+ +E+LV G H + W ++G +VP A+ VG +G PLYV R + + + GK +P H C Y+PYGG+EHR+E EVL Sbjct: 5 TWISTNVYGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV-----GDH--FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVL 142 HSP 2 Score: 82.4185 bits (202), Expect = 3.823e-18 Identity = 49/147 (33.33%), Postives = 76/147 (51.70%), Query Frame = 1 Query: 298 LSWVKANNGSIPPKAVRAGGQT------FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 717 SW+ ++ GS+PP A++ G QT +V R G PGK + +++ +GG EH YE+LV+ W ++G + G +VGG + DG +YV R++ E ++ K P GCAY+PYGG E + E+L Sbjct: 76 FSWIPSSGGSVPPHAIQVG-QTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQPET--------WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213 HSP 3 Score: 77.411 bits (189), Expect = 2.511e-16 Identity = 48/144 (33.33%), Postives = 72/144 (50.00%), Query Frame = 1 Query: 301 SWVKANNGSIPPKAV-----RAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGA-LVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +W+ ++ I P V G Q +V RA H+G +P K + +V +GG E K+ YE+L GY W + NVP A L G +DG PL++ R+ + GK + H C Y+P+ GEE R+++ EVL Sbjct: 147 TWIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAG-------YGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVL 283
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0087792) HSP 1 Score: 102.064 bits (253), Expect = 1.785e-25 Identity = 59/145 (40.69%), Postives = 87/145 (60.00%), Query Frame = 1 Query: 301 SWVKAN-NGSIPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGAL-VGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +W+ N GS+PP A+ AG + FV RA H+G+ +P K V G + +V +GG E +K+ +E+LV G H + W ++G +VP A+ VG +G PLYV R + + + GK +P H C Y+PYGG+EHR+E EVL Sbjct: 5 TWISTNVYGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV-----GDH--FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVL 142 HSP 2 Score: 82.4185 bits (202), Expect = 3.823e-18 Identity = 49/147 (33.33%), Postives = 76/147 (51.70%), Query Frame = 1 Query: 298 LSWVKANNGSIPPKAVRAGGQT------FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 717 SW+ ++ GS+PP A++ G QT +V R G PGK + +++ +GG EH YE+LV+ W ++G + G +VGG + DG +YV R++ E ++ K P GCAY+PYGG E + E+L Sbjct: 76 FSWIPSSGGSVPPHAIQVG-QTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQPET--------WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213 HSP 3 Score: 77.411 bits (189), Expect = 2.511e-16 Identity = 48/144 (33.33%), Postives = 72/144 (50.00%), Query Frame = 1 Query: 301 SWVKANNGSIPPKAV-----RAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGA-LVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +W+ ++ I P V G Q +V RA H+G +P K + +V +GG E K+ YE+L GY W + NVP A L G +DG PL++ R+ + GK + H C Y+P+ GEE R+++ EVL Sbjct: 147 TWIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAG-------YGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVL 283
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG10527 (gene:FBgn0034583 transcript:FBtr0071541) HSP 1 Score: 96.6709 bits (239), Expect = 2.745e-23 Identity = 53/142 (37.32%), Postives = 76/142 (53.52%), Query Frame = 1 Query: 301 SWVKANNGSIPPKAVRAG----GQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGG-LNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEV 711 WV A NG +PP A+ G Q ++ARA+H+G IPGK + +V++GG EH YE+L G +W + N+P AL G +G PL++ R+ + + VGK P HGC Y+PYGGEE + E+ Sbjct: 158 CWVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLCAG-------GGQWLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCYIPYGGEELAYKEFEI 292 HSP 2 Score: 51.6026 bits (122), Expect = 1.942e-7 Identity = 28/61 (45.90%), Postives = 36/61 (59.02%), Query Frame = 1 Query: 535 NGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 717 NGE VP AL GG + LY+AR+ E ++ GK +P HG Y+ +GG EH EVLC Sbjct: 164 NGE-VPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLC 223
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339105) HSP 1 Score: 92.4337 bits (228), Expect = 7.649e-22 Identity = 56/155 (36.13%), Postives = 83/155 (53.55%), Query Frame = 1 Query: 271 QYGAGVELSLSWVKANNGS-IPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 Q G V + +WV ++ S +PP AV G + +V R+ H+G+ +P K V +V++GG EH K +YE+LV +G+ W ++ VP A+ G G PLYV R + VGK +P HGC Y+P+GG+E R+ EVL Sbjct: 5 QPGGVVTVDNTWVHSSPYSPLPPYAVIGGHDSDRTPIYVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVG-------QGFAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVL 152 HSP 2 Score: 77.7962 bits (190), Expect = 2.028e-16 Identity = 49/145 (33.79%), Postives = 68/145 (46.90%), Query Frame = 1 Query: 304 WVKANNGSIPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTN-GENVPKGALVGG-LNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 717 WV A+ PP AV AG + + RA H+G+ +P K V +V FGG E K YE+L GY + W N G ++P A+ G +G LY R + + G C Y+PYGG E R+ + E+LC Sbjct: 160 WVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVLT---------GYGYVWMNCGHSIPPNAVTTGRARNGELLYYGRGHYQGSLTPGLISASQRCLYIPYGGREIRINSYEILC 295 HSP 3 Score: 72.4034 bits (176), Expect = 1.451e-14 Identity = 45/145 (31.03%), Postives = 72/145 (49.66%), Query Frame = 1 Query: 298 LSWVKANNGSIPPKAVRAGGQ-----TFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +WV +++G +PP AVR+G +V R H G GK + +++ FGG E N YE+L++ + W + P GA++ G + D P+Y R+ E +M+ K P G AY+ +GG E + + EVL Sbjct: 86 FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIK------QQHDVWVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVL 224
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339104) HSP 1 Score: 90.5077 bits (223), Expect = 3.697e-21 Identity = 53/145 (36.55%), Postives = 79/145 (54.48%), Query Frame = 1 Query: 301 SWVKANNGS-IPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +WV ++ S +PP AV G + +V R+ H+G+ +P K V +V++GG EH K +YE+LV +G+ W ++ VP A+ G G PLYV R + VGK +P HGC Y+P+GG+E R+ EVL Sbjct: 4 TWVHSSPYSPLPPYAVIGGHDSDRTPIYVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVG-------QGFAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVL 141 HSP 2 Score: 77.7962 bits (190), Expect = 1.558e-16 Identity = 49/145 (33.79%), Postives = 68/145 (46.90%), Query Frame = 1 Query: 304 WVKANNGSIPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTN-GENVPKGALVGG-LNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 717 WV A+ PP AV AG + + RA H+G+ +P K V +V FGG E K YE+L GY + W N G ++P A+ G +G LY R + + G C Y+PYGG E R+ + E+LC Sbjct: 149 WVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVLT---------GYGYVWMNCGHSIPPNAVTTGRARNGELLYYGRGHYQGSLTPGLISASQRCLYIPYGGREIRINSYEILC 284 HSP 3 Score: 72.4034 bits (176), Expect = 1.411e-14 Identity = 45/145 (31.03%), Postives = 72/145 (49.66%), Query Frame = 1 Query: 298 LSWVKANNGSIPPKAVRAGGQT-----FVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLN-DGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 +WV +++G +PP AVR+G +V R H G GK + +++ FGG E N YE+L++ + W + P GA++ G + D P+Y R+ E +M+ K P G AY+ +GG E + + EVL Sbjct: 75 FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIK------QQHDVWVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVL 213
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: Q5DHE4 (SJCHGC04453 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 140.584 bits (353), Expect = 3.677e-38 Identity = 65/154 (42.21%), Postives = 98/154 (63.64%), Query Frame = 1 Query: 268 SQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 729 ++ G G+++ LSWV A +G++P A+ G +V R+KHDG +IPGK V G+N + G E YE+L ++S+ G +KGYEW+ +G NVPK A++ G++ PLY+AR + ++ VGK + H CAY P+GG EH V + EVL + K Sbjct: 2 AKIGKGIQIPLSWVPATDGNVPHNAINVSGDIYVIRSKHDGHWIPGKAVIGHNTAYYPMNGQEIKTREYEVLCDTSLSGHNKGYEWESESGGNVPKNAIIAGMSGNDPLYIARGSVNGEVCVGKVHYGHNCAYFPWGGNEHSVNDYEVLVWKKK 155
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A0R3T053 (DUF3421 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS165 PE=4 SV=1) HSP 1 Score: 139.428 bits (350), Expect = 1.190e-37 Identity = 67/155 (43.23%), Postives = 96/155 (61.94%), Query Frame = 1 Query: 268 SQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGL-NDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 729 ++ G G ++ +SWV +PP A+ G +VAR DG+ IPGK+VE Y +VS+GG EH EIL ++ + YEW+ +G NVPK A+VGG+ DG PLY+ R+ ++ + VGK + H CAY+P+GGEEH VE+ +VLC K Sbjct: 2 AKVGKGFQIGVSWVPLKGRGVPPGAIDIGDGVYVARGCKDGEQIPGKYVEKYGTCYVSYGGAEHELKECEILCDTCISSTGCCYEWREDSGGNVPKNAIVGGIAEDGEPLYICRAKVDGEKCVGKVHNGHDCAYIPHGGEEHSVEDYKVLCLRKK 156
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A4Z2D8K2 (Natterin-4 OS=Schistosoma japonicum OX=6182 GN=EWB00_003361 PE=4 SV=1) HSP 1 Score: 139.043 bits (349), Expect = 1.478e-37 Identity = 65/149 (43.62%), Postives = 96/149 (64.43%), Query Frame = 1 Query: 268 SQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 ++ G G+++ LSWV A +G++P AV G +V R+KHDG +IPGK V G+N + G E YE+L ++S+ G +KGYEW+ +G NVPK A++ G++ PLY+AR + ++ VGK + H CAY P+GG EH V + EVL Sbjct: 2 AKIGKGIQIPLSWVPATDGNVPHNAVNVSGDIYVIRSKHDGHWIPGKAVIGHNTAYYPMNGQEIKTREYEVLCDTSLSGHNKGYEWESESGGNVPKNAIIAGMSGNDPLYIARGSVNGEVCVGKVHYGHNCAYFPWGGNEHSVNDYEVL 150
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A5J4NEV5 (DUF3421 domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0014321 PE=4 SV=1) HSP 1 Score: 137.502 bits (345), Expect = 5.522e-37 Identity = 67/150 (44.67%), Postives = 92/150 (61.33%), Query Frame = 1 Query: 268 SQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLND-GAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVL 714 S+ G G ++ LSW+ A G +PPKA+ AGG +V R H G IPGK + + + S G EH + YE+L ++ Y W++ +G +VPK A+V GL D G+PLY+AR I + VVGK + H CAY PYGGEEHR+ + EVL Sbjct: 2 SKIGRGFQVGLSWIHAEGGHVPPKAIDAGGGVYVGRMHHAGDLIPGKVIPRFGHAYCSHAGQEHTHSSYEVLCDTCAPSTRHCYRWEYASGGHVPKSAIVAGLADNGSPLYIARVEINGERVVGKVHDGHPCAYFPYGGEEHRMPDYEVL 151
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H8B2 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012514g1 PE=4 SV=1) HSP 1 Score: 136.732 bits (343), Expect = 1.218e-36 Identity = 72/155 (46.45%), Postives = 101/155 (65.16%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHR-VENAEVL 714 MS+V+ G+EL LSW A+NG IP AV+AG +VARAKH+G+++PGK G+ +V +G E + YE+LV S + GY+W G +VPKGALV G ++GAPLYVAR+ E ++ GK P CAY+ +GG+EH+ V++ EVL Sbjct: 1 MSRVNHNDIEGIELCLSWHPASNGDIPAGAVKAGEDCYVARAKHEGEWVPGKLSAGHTSAYVPYGDEEVPYSEYEVLVSSEIGHFGCGYQWLPAKGNSVPKGALVSGYDNGAPLYVARATFEGEVCCGKAQPDLECAYISWGGKEHKIVDDYEVL 155
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074396.1 (SMESG000074396.1) HSP 1 Score: 331.643 bits (849), Expect = 6.738e-117 Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 735 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV Sbjct: 1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 161
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074378.1 (SMESG000074378.1) HSP 1 Score: 331.643 bits (849), Expect = 6.738e-117 Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 735 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV Sbjct: 1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 161
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074377.1 (SMESG000074377.1) HSP 1 Score: 191.43 bits (485), Expect = 1.701e-61 Identity = 92/159 (57.86%), Postives = 119/159 (74.84%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 729 MSKVSSQYG GVE+ +SW KA+NG IP + V+AGG+ FV RA+ +GQYIPGK EG+ V+VS+GG E N YE LVE+++L + G++WK+ +G NVP GA+VGG ++G PLY+ARS + + VVGKY+P HGCAY P+GGEEHR EVLC K Sbjct: 1 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVL-HNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 158
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029910.1 (SMESG000029910.1) HSP 1 Score: 176.792 bits (447), Expect = 9.767e-56 Identity = 80/161 (49.69%), Postives = 116/161 (72.05%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 735 MSKV +QYG VE+S+SW +A++GSIP AV+A G FVARAKH+G+YIPGK V G++ VFV + G E K++Y++LV++S+L GYEWKW+ +P A++GG ++G PL++ +S +E + VVGK++P HGCA++PY G E E+LC KH+ Sbjct: 1 MSKVITQYGRAVEISVSWQEAHDGSIPLHAVKADGSCFVARAKHNGEYIPGKLVTGHSHVFVCYDGKEITKSHYQVLVDTSILPT-TGYEWKWSEDLEIPPNAIIGGTDNGQPLFIVKSIVEGETVVGKFHPDHGCAHVPYEGLEFEKNEFEILCSLKKHL 160
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029913.1 (SMESG000029913.1) HSP 1 Score: 175.637 bits (444), Expect = 2.487e-55 Identity = 82/161 (50.93%), Postives = 114/161 (70.81%), Query Frame = 1 Query: 253 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 735 MSK+ S+YG VE+S+SW+ +G+IP KAV AG +VARAKH +YIPGKFV+G+ +VS+ G E AK+ Y+ILV+SS+LGG EW +T G +P ++VGG ++ PLY+A+S I ++VVGKY P+ C + PYGG+EHR E+LC TTK + Sbjct: 1 MSKIVSEYGRSVEISVSWIDCQDGNIPDKAVSAGDSCYVARAKHLDEYIPGKFVQGHTSAYVSYDGKEIAKSEYQILVDSSILGG-PSIEWHYTRGNQIPYRSIVGGTDNERPLYIAKSAINGEVVVGKYLPEFACGFFPYGGKEHRKSMVEILCCTTKQL 160 The following BLAST results are available for this feature:
BLAST of DUF3421 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of DUF3421 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005863 ID=SMED30005863|Name=DUF3421 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=777bpback to top protein sequence of SMED30005863-orf-1 >SMED30005863-orf-1 ID=SMED30005863-orf-1|Name=SMED30005863-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|