NPHS1-6
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005845 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of NPHS1-6 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 224.942 bits (572), Expect = 1.052e-58 Identity = 262/1172 (22.35%), Postives = 455/1172 (38.82%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD---NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKED--ESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQ-----------VGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKT----IMTNTM-SADNEKENNQPTYPIIQPNKLQDNTILF 3773
V +G++ LRC V VQW +LLG D IPG P YR+ G +GE L I + D YECQV + P L ++ ++ P P++ P ++ Q+ CVS + +P L+ I D N N ++L + + + ++ C S+ A E P + S + V PPG P +++ ++ + C + G P +L W V + + Q ++++ +V T+ ++HGAQL C A N V Q + L + P+ ++I +G EN + T++C + ++W + + P+E+ + S + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W++DS+ + S + Q+ G+ + ++++ +DN+ K+ C+A +S + L V FPP + + + + A + G L L C + SNPP + W ++ G+ + + P + F GS + S +DH V C + E + + ++NV + P+F G + +E+G++ L + ANP + + W +LS H I +S A L + N D Y L N G ++ + L++ Y P I + + + + ++ C+V +NP P M + W +L G+ D S D M K T L I+ G Y+C+V+NG+ + + L F PQ+ +K AAG+ +L C R P+ TW + + T + G + + L I N+ D+ + C A N GSD I++ S PD P ++VV+ + + + W GFDGG Q F +R+ E T + Y DV +F +TGL+ T Y + + + N+ + K I T + E E+ PT P P+ L +LF
Sbjct: 43 VVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVG-RSEMGPELVSPRVILSILVP--PKLLL--LTPEAGTMVTWVAGQEY--VVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEA---PIKASFTVNVLF----PPGPP-VIEWPGLDEGHVRAGQSLELPCVAR-GGNPLATLQWLKNGQPVSTAWGTE-------------HTQAVARSVLVMTVR-----PEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII-----------LGSASQTEN-------KNVTLSCVSKSSRPRVLLRWW------LGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQ--------LAVQFPPTNVTILANASA-LRPGDALNLTCVSVSSNPPVNLSW--DKEGERLEGVAAPPRRA-----------------PFKGSAAARSVLLQVSSRDHGQRVTCRAHS---AELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGA-LHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGM-FNWERL--GEDEEDQSLDD-----------------------------------------------MEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWT--KNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY-EALGTPGFHYVDVVPPQAT---TFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLF 1067
BLAST of NPHS1-6 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 221.476 bits (563), Expect = 9.893e-58 Identity = 250/1123 (22.26%), Postives = 438/1123 (39.00%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD---NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKED--ESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQ-----------VGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNS 3641
V +G++ LRC V VQW +LLG D IPG P YR+ G +GE L I + D YECQV + P L ++ ++ P P++ P ++ Q+ CVS + +P L+ I D N N ++L + + + ++ C S+ A E P + S + V PPG P +++ ++ + C + G P +L W V + + Q ++++ +V T+ ++HGAQL C A N V Q + L + P+ ++I +G EN + T++C + ++W + + P+E+ + S + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W++DS+ + S + Q+ G+ + ++++ +DN+ K+ C+A +S + L V FPP + + + + A + G L L C + SNPP + W ++ G+ + + P + F GS + S +DH V C + E + + ++NV + P+F G + +E+G++ L + ANP + + W +LS H I +S A L + N D Y L N G ++ + L++ Y P I + + + + ++ C+V +NP P M + W +L G+ D S D M K T L I+ G Y+C+V+NG+ + + L F PQ+ +K AAG+ +L C R P+ TW + + T + G + + L I N+ D+ + C A N GSD I++ S PD P ++VV+ + + + W GFDGG Q F +R+ E T + Y DV +F +TGL+ T Y + + + N+
Sbjct: 43 VVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVG-RSEMGPELVSPRVILSILVP--PKLLL--LTPEAGTMVTWVAGQEYV--VNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEA---PIKASFTVNVLF----PPGPP-VIEWPGLDEGHVRAGQSLELPCVAR-GGNPLATLQWLKNGQPVSTAWGTE-------------HTQAVARSVLVMTVR-----PEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII-----------LGSASQTEN-------KNVTLSCVSKSSRPRVLLRWW------LGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQ--------LAVQFPPTNVTILANASA-LRPGDALNLTCVSVSSNPPVNLSW--DKEGERLEGVAAPPRR-----------------APFKGSAAARSVLLQVSSRDHGQRVTCRAHS---AELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGA-LHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGM-FNWERL--GEDEEDQSLDD-----------------------------------------------MEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWT--KNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY-EALGTPGFHYVDVVPPQAT---TFTLTGLQPSTRYRVWLLASNA 1018
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 71.633 bits (174), Expect = 8.133e-12 Identity = 74/255 (29.02%), Postives = 107/255 (41.96%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLS---LKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW 1103
V G TL C + G V W + LG R++ P Y VVG+ + GEH L+I ++D +YECQ +Q + P +L VLV PD P I G VI S++ TC +DN +P W +I S L S +S + ++G I CR +N+A ++ T V I I PP V+ P V++DN++ T S PA + +RW
Sbjct: 59 VVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRP-----ARLTVLVPPDDPVIL-------GGPVI--SLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKE-------TSVTIDIQHPPLVNLSVEPQP------VLEDNVV-TFHCSAKANPAVT-QYRW 284
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 72.0182 bits (175), Expect = 8.348e-12 Identity = 74/255 (29.02%), Postives = 107/255 (41.96%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLS---LKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW 1103
V G TL C + G V W + LG R++ P Y VVG+ + GEH L+I ++D +YECQ +Q + P +L VLV PD P I G VI S++ TC +DN +P W +I S L S +S + ++G I CR +N+A ++ T V I I PP V+ P V++DN++ T S PA + +RW
Sbjct: 59 VVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRP-----ARLTVLVPPDDPVIL-------GGPVI--SLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKE-------TSVTIDIQHPPLVNLSVEPQP------VLEDNVV-TFHCSAKANPAVT-QYRW 284
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 71.633 bits (174), Expect = 1.175e-11 Identity = 74/255 (29.02%), Postives = 107/255 (41.96%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLS---LKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW 1103
V G TL C + G V W + LG R++ P Y VVG+ + GEH L+I ++D +YECQ +Q + P +L VLV PD P I G VI S++ TC +DN +P W +I S L S +S + ++G I CR +N+A ++ T V I I PP V+ P V++DN++ T S PA + +RW
Sbjct: 59 VVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRP-----ARLTVLVPPDDPVIL-------GGPVI--SLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKE-------TSVTIDIQHPPLVNLSVEPQP------VLEDNVV-TFHCSAKANPAVT-QYRW 284
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 117.857 bits (294), Expect = 4.098e-26 Identity = 184/838 (21.96%), Postives = 326/838 (38.90%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKV---QWRA-VPVLLGYDRN--IPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQ-IFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYS---RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQI-VDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISY-QSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFS----PNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSF-IAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE--VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPV---DIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTK 2762
Q F E P + +A G + T RC + QW++ LLGY + +PG E L+I++V + D G YECQ+ HP L PQ +++ +QPN I ++K++ TCV N +P+ + W+++ ++ + A LN + + L +++ + G VI C + T++ ++T + + PP P + + NP + DN+ + C S G P + W + N R ++++T D +++K + + + ++ A+ C A N + A+KL + + P V + G+ N+ YG + I C + P + I W+ + P P +F+ + S S +++ + Q ++C A + S + + I P+ P I G K + +TC GGNP EL W+R S+ + + + TL + + ++N ++K C+A + +L + S L V +PP + ++ + + GK +L C SNP A + W+ +R T N+ L S N + G +++ P+ + V C +P++ +H V +NV + P+ + EG S NL NP I + W +N H+I A+ + + K + D YTL N G + ITI L + Y I LEC P+ +M + W K
Sbjct: 19 QFFLESPSNLSTIA-GESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQML-----HPEEGPIRAKSFLNIIVPPQLVYFSNYQPNS----IIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIR-------ITTNTTLDVLFPPSDPTVEILRNP---SALRSGDNVTIAC-SVTGGNPPPDVFWYHE--------NKRLQSHSTLD----------TRSKEIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVEL-----------FGES----NIRYGSSAN---IQCKSLPSNPASQITWIINGRSVPTPT-QREFV-----VENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKAPIITGLEDRKFFE-GDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLALRVDRTMNTQRLK--CEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRA-------------TDDNKVHLGDVSLNETT-RDNGFNVENVLSFTPTEEYDGTVVHCIANHPEW--KHSV-NTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD----HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-WYK 766 HSP 2 Score: 83.5741 bits (205), Expect = 1.084e-15 Identity = 68/275 (24.73%), Postives = 121/275 (44.00%), Query Frame = 3
Query: 2889 KAEWKGTPEKSNFKYFSK---CMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANS-KYRINITRFRTGFYVAQLKIVNLQRTDHGSYV-CKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD 3698
+ E G P K+N ++ K + ++ + G Y C +NGI L P++L + +++K AG LG C + + P+ + W + + NS KY I T+ Y + L I N+ D+ V C A N G+D I + + PD P + + N + + I ++W FDGGS Q F+V+++ ++ ++ V + T + LS GL + Y I S N++ S ++ ++ T++ D
Sbjct: 749 ECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVW--EKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHL---VNTTHTNLRLS----GLATANTYFFQIRSINARGFASSWTSPVIFATLNED 1014
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 111.694 bits (278), Expect = 3.299e-24 Identity = 186/851 (21.86%), Postives = 328/851 (38.54%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKV---QWRA-VPVLLGYDRN--IPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQ-IFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYS---RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQI-VDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISY-QSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFS----PNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDS--------KPIQSSFI------AQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE--VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPV---DIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTK 2762
Q F E P + +A G + T RC + QW++ LLGY + +PG E L+I++V + D G YECQ+ HP L PQ +++ +QPN I ++K++ TCV N +P+ + W+++ ++ + A LN + + L +++ + G VI C + T++ ++T + + PP P + + NP + DN+ + C S G P + W + N R ++++T D +++K + + + ++ A+ C A N + A+KL + + P V + G+ N+ YG + I C + P + I W+ + P P +F+ + S S +++ + Q ++C A + S + + I P+ P I G K + +TC GGNP EL W+R S K ++ S + + TL + + ++N ++K C+A + +L + S L V +PP + ++ + + GK +L C SNP A + W+ +R T N+ L S N + G +++ P+ + V C +P++ +H V +NV + P+ + EG S NL NP I + W +N H+I A+ + + K + D YTL N G + ITI L + Y I LEC P+ +M + W K
Sbjct: 19 QFFLESPSNLSTIA-GESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQML-----HPEEGPIRAKSFLNIIVPPQLVYFSNYQPNS----IIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIR-------ITTNTTLDVLFPPSDPTVEILRNP---SALRSGDNVTIAC-SVTGGNPPPDVFWYHE--------NKRLQSHSTLD----------TRSKEIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVEL-----------FGES----NIRYGSSAN---IQCKSLPSNPASQITWIINGRSVPTPT-QREFV-----VENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKAPIITGLEDRKFFE-GDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLK--CEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRA-------------TDDNKVHLGDVSLNETT-RDNGFNVENVLSFTPTEEYDGTVVHCIANHPEW--KHSV-NTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD----HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-WYK 779 HSP 2 Score: 83.5741 bits (205), Expect = 1.126e-15 Identity = 68/275 (24.73%), Postives = 121/275 (44.00%), Query Frame = 3
Query: 2889 KAEWKGTPEKSNFKYFSK---CMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANS-KYRINITRFRTGFYVAQLKIVNLQRTDHGSYV-CKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD 3698
+ E G P K+N ++ K + ++ + G Y C +NGI L P++L + +++K AG LG C + + P+ + W + + NS KY I T+ Y + L I N+ D+ V C A N G+D I + + PD P + + N + + I ++W FDGGS Q F+V+++ ++ ++ V + T + LS GL + Y I S N++ S ++ ++ T++ D
Sbjct: 762 ECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVW--EKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHL---VNTTHTNLRLS----GLATANTYFFQIRSINARGFASSWTSPVIFATLNED 1027
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: igcm-1 (ImmunoGlobulin-like Cell adhesion Molecule family [Source:UniProtKB/TrEMBL;Acc:Q9TXI8]) HSP 1 Score: 95.1301 bits (235), Expect = 3.558e-19 Identity = 104/450 (23.11%), Postives = 182/450 (40.44%), Query Frame = 3
Query: 2445 ANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLI-IRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEII---RQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVK---SQFEMTSLLIIYDVDRNDVGLYRCVVNNG------------IRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRF--RTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE----------KSPTSLYRY----EDVASDDTKITLS-FIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNT 3686
A P YTW S+ + + K +RNA+ D Y+ ND GE +I + + I + I Q LEC Q P+ P K K+ +P S T E SKC+ + +Q ++L + + +D G + CV +NG I + K + L ++F P+ S AA ++G + + C + + P P+ W F ++ +S N ++ + + Y L+I ++Q +D+G YVC+A NG G D IE+ TS P +PL++Q ++ + + + + W+ FDGG+ QT+ + ++ +P S +D D T ++ + F TGL + Y + I + + K G S+F+ ++ +T
Sbjct: 452 AGNPSASYTWASPSSG----GIFGLEGHTEKSFEVRNAQLSDNGVYSCKAYNDLGEGKIGTVMITVIEKARISSPLATERIFTSGEQGKILECEAQGYPS-------------------------------------PVIKWLKDGRPVKQSIYASSATSE-------SKCLPEDFCTQTVASTLTLAGPLKWSDKGNFTCVADNGSDKTDKDSSSWTIVRVLHKPVFLNRKF-------PEKSLAAADIGSQAIIRCKVSARPEPEFHWMF--RDSEISENERHSFHTVGITGKPDEYEQLLQIESVQESDYGEYVCRATNGNGGDHVVIELRKTSPPALPLDLQKLSATSNSVLLGWVPQFDGGADQTYVLETRKIDPFTGEIDGNAPVSKLNINAVQQDQEIDGTLVSKNIFNFTGLTPLSTYNLRIQAVSEK-GESEFTPVLIAST 843
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 82.8037 bits (203), Expect = 1.518e-15 Identity = 68/275 (24.73%), Postives = 121/275 (44.00%), Query Frame = 3
Query: 2889 KAEWKGTPEKSNFKYFSK---CMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANS-KYRINITRFRTGFYVAQLKIVNLQRTDHGSYV-CKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD 3698
+ E G P K+N ++ K + ++ + G Y C +NGI L P++L + +++K AG LG C + + P+ + W + + NS KY I T+ Y + L I N+ D+ V C A N G+D I + + PD P + + N + + I ++W FDGGS Q F+V+++ ++ ++ V + T + LS GL + Y I S N++ S ++ ++ T++ D
Sbjct: 252 ECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVW--EKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHL---VNTTHTNLRLS----GLATANTYFFQIRSINARGFASSWTSPVIFATLNED 517 HSP 2 Score: 51.9878 bits (123), Expect = 4.050e-6 Identity = 66/292 (22.60%), Postives = 110/292 (37.67%), Query Frame = 3
Query: 1902 NKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE--VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPV---DIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTK 2762
N + C+A + +L + S ++ V +PP + ++ + + GK +L C SNP A + W+ +R T N+ L S N + G +++ P+ + V C +P++ +H V +NV + P+ + EG S NL NP I + W +N H+I A+ + + K + D YTL N G + ITI L + Y I LEC P+ +M + W K
Sbjct: 2 NTQRLKCEATNAALDEPLIESQYLS--VYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRA-------------TDDNKVHLGDVSLNETT-RDNGFNVENVLSFTPTEEYDGTVVHCIANHPEW--KHSV-NTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD----HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-WYK 269
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 82.8037 bits (203), Expect = 1.652e-15 Identity = 68/275 (24.73%), Postives = 121/275 (44.00%), Query Frame = 3
Query: 2889 KAEWKGTPEKSNFKYFSK---CMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANS-KYRINITRFRTGFYVAQLKIVNLQRTDHGSYV-CKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD 3698
+ E G P K+N ++ K + ++ + G Y C +NGI L P++L + +++K AG LG C + + P+ + W + + NS KY I T+ Y + L I N+ D+ V C A N G+D I + + PD P + + N + + I ++W FDGGS Q F+V+++ ++ ++ V + T + LS GL + Y I S N++ S ++ ++ T++ D
Sbjct: 447 ECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVW--EKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHL---VNTTHTNLRLS----GLATANTYFFQIRSINARGFASSWTSPVIFATLNED 712 HSP 2 Score: 75.485 bits (184), Expect = 2.806e-13 Identity = 92/398 (23.12%), Postives = 158/398 (39.70%), Query Frame = 3
Query: 1593 KINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSF-IAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE--VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIIN--YNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPV---DIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTK 2762
+++ + Q ++C A + S + + I P+ P I G K + +TC GGNP EL W+R S+ + + + TL + + ++N ++K C+A + +L + S L V +PP + ++ + + GK +L C SNP A + W+ +R T N+ L S N + G +++ P+ + V C +P++ +H V +NV + P+ ++N + EG S NL NP I + W +N H+I A+ + + K + D YTL N G + ITI L + Y I LEC P+ +M + W K
Sbjct: 96 ELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKAPIITGLEDRKFFE-GDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLALRVDRTMNTQRLK--CEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRA-------------TDDNKVHLGDVSLNETT-RDNGFNVENVLSFTPTEEYDGTVVHCIANHPEW--KHSV-NTSFPLNVMYPPKM--LVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD----HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-WYK 464
BLAST of NPHS1-6 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 257.299 bits (656), Expect = 3.639e-69 Identity = 271/1131 (23.96%), Postives = 461/1131 (40.76%), Query Frame = 3
Query: 312 IQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFT--CVSDNGQPKVKFQWFLNNNLIQDNENSALLEELN-------YSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTA-VKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSG--IKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYV--DCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNV--HSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFE-MTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGT 3653
+Q F P + ++ +G+ LRC V N+ GKVQW LG+ IPG P Y V+ + + L+I N T++D Y+CQV P +P +R KL ++ P I +G+ N ++ + R T C+++N P + WF + ++A + +N + S L L+ D C ++A P + +V + + PPG P + +T + + C S G P L W Y N SPQ +S+N V T A +++GA L C A N + + A + L +L+ P V +S G N+ + G+ ++C T P +A I W + P N+ + + D + +S I + I+ Q C A + ++V +++ Y P P + GYN G + ++ CS+ GGNP P LQW+++ K I + ++ V ++ T ++ + + +DN Y C+ ++ ++ PL + + TL V FPP T+ + S+ + G KL C + SNPPA + W + L RP G +GGS+ S E+Y + LD + C+ N + + + I +++ + P+FE + F EG + L NP + YTW ++ +S ++ + + +L I D Y +N +G + + I + + Y P I R + + A L C +Q+ P +W++ GY D+++ + S FE T+LL I V+R+D+G + C+V+N + + L + P+I P F++ A LG L C + P P W + + +K++ + + + L I N D+G Y C N G S +E S S PD PL+++V N S + ++++W GFDGG F++R K+ Y+Y D I+L GLK Y S+ + N G+
Sbjct: 38 VQKFHLTPHDL-QILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAG-NPAIRANAKLSIVAAPS--SIVIEGYARNA------RVEVEERQNLTLHCIAENANPAAEIVWFQGEVPV----STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKAL-----PPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHR--GQEVQIACRSR-GGNPPAQLTW----------YRN-----GVAISSPQRTSGRLSEN--VYKFTAAA---EDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLS--------------GANQ----AKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTY----KTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAP--SKLVDSKITSELSLLVNASDNNAIYKCKVQNAAID-IPLFATK-TLGVHFPPETVKI-SVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRP-------------------GLWGGSV--STLEMYVNITQDLDGIVYTCQSHNEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLE-AAHVRWSR----DGY-DLATRTI-------------------------------------------------SSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDK-YKYVDAKPGHQNISLD----GLKPGATYYFSVMAANEAGGS 1015
BLAST of NPHS1-6 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0345419) HSP 1 Score: 257.299 bits (656), Expect = 3.639e-69 Identity = 271/1131 (23.96%), Postives = 461/1131 (40.76%), Query Frame = 3
Query: 312 IQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFT--CVSDNGQPKVKFQWFLNNNLIQDNENSALLEELN-------YSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTA-VKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSG--IKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYV--DCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNV--HSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFE-MTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGT 3653
+Q F P + ++ +G+ LRC V N+ GKVQW LG+ IPG P Y V+ + + L+I N T++D Y+CQV P +P +R KL ++ P I +G+ N ++ + R T C+++N P + WF + ++A + +N + S L L+ D C ++A P + +V + + PPG P + +T + + C S G P L W Y N SPQ +S+N V T A +++GA L C A N + + A + L +L+ P V +S G N+ + G+ ++C T P +A I W + P N+ + + D + +S I + I+ Q C A + ++V +++ Y P P + GYN G + ++ CS+ GGNP P LQW+++ K I + ++ V ++ T ++ + + +DN Y C+ ++ ++ PL + + TL V FPP T+ + S+ + G KL C + SNPPA + W + L RP G +GGS+ S E+Y + LD + C+ N + + + I +++ + P+FE + F EG + L NP + YTW ++ +S ++ + + +L I D Y +N +G + + I + + Y P I R + + A L C +Q+ P +W++ GY D+++ + S FE T+LL I V+R+D+G + C+V+N + + L + P+I P F++ A LG L C + P P W + + +K++ + + + L I N D+G Y C N G S +E S S PD PL+++V N S + ++++W GFDGG F++R K+ Y+Y D I+L GLK Y S+ + N G+
Sbjct: 38 VQKFHLTPHDL-QILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAG-NPAIRANAKLSIVAAPS--SIVIEGYARNA------RVEVEERQNLTLHCIAENANPAAEIVWFQGEVPV----STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKAL-----PPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHR--GQEVQIACRSR-GGNPPAQLTW----------YRN-----GVAISSPQRTSGRLSEN--VYKFTAAA---EDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLS--------------GANQ----AKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTY----KTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAP--SKLVDSKITSELSLLVNASDNNAIYKCKVQNAAID-IPLFATK-TLGVHFPPETVKI-SVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRP-------------------GLWGGSV--STLEMYVNITQDLDGIVYTCQSHNEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLE-AAHVRWSR----DGY-DLATRTI-------------------------------------------------SSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDK-YKYVDAKPGHQNISLD----GLKPGATYYFSVMAANEAGGS 1015
BLAST of NPHS1-6 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 256.914 bits (655), Expect = 4.241e-69 Identity = 271/1131 (23.96%), Postives = 461/1131 (40.76%), Query Frame = 3
Query: 312 IQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFT--CVSDNGQPKVKFQWFLNNNLIQDNENSALLEELN-------YSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTA-VKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSG--IKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYV--DCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNV--HSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFE-MTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGT 3653
+Q F P + ++ +G+ LRC V N+ GKVQW LG+ IPG P Y V+ + + L+I N T++D Y+CQV P +P +R KL ++ P I +G+ N ++ + R T C+++N P + WF + ++A + +N + S L L+ D C ++A P + +V + + PPG P + +T + + C S G P L W Y N SPQ +S+N V T A +++GA L C A N + + A + L +L+ P V +S G N+ + G+ ++C T P +A I W + P N+ + + D + +S I + I+ Q C A + ++V +++ Y P P + GYN G + ++ CS+ GGNP P LQW+++ K I + ++ V ++ T ++ + + +DN Y C+ ++ ++ PL + + TL V FPP T+ + S+ + G KL C + SNPPA + W + L RP G +GGS+ S E+Y + LD + C+ N + + + I +++ + P+FE + F EG + L NP + YTW ++ +S ++ + + +L I D Y +N +G + + I + + Y P I R + + A L C +Q+ P +W++ GY D+++ + S FE T+LL I V+R+D+G + C+V+N + + L + P+I P F++ A LG L C + P P W + + +K++ + + + L I N D+G Y C N G S +E S S PD PL+++V N S + ++++W GFDGG F++R K+ Y+Y D I+L GLK Y S+ + N G+
Sbjct: 38 VQKFHLTPHDL-QILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAG-NPAIRANAKLSIVAAPS--SIVIEGYARNA------RVEVEERQNLTLHCIAENANPAAEIVWFQGEVPV----STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKAL-----PPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHR--GQEVQIACRSR-GGNPPAQLTW----------YRN-----GVAISSPQRTSGRLSEN--VYKFTAAA---EDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLS--------------GANQ----AKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTY----KTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAP--SKLVDSKITSELSLLVNASDNNAIYKCKVQNAAID-IPLFATK-TLGVHFPPETVKI-SVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRP-------------------GLWGGSV--STLEMYVNITQDLDGIVYTCQSHNEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLE-AAHVRWSR----DGY-DLATRTI-------------------------------------------------SSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDK-YKYVDAKPGHQNISLD----GLKPGATYYFSVMAANEAGGS 1015
BLAST of NPHS1-6 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 249.21 bits (635), Expect = 2.993e-66 Identity = 270/1120 (24.11%), Postives = 451/1120 (40.27%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSR----SRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQ--STAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNP---AINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEII---RQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRN 3638
Q F+ P + +V +G+ A +RC V N G VQW LG+ IPG P Y V+G +G + L ISN ++ D Y+CQV P + + +R KL V+ P +I KG+ N ++++ + C+ N +P + W+ N + + +EE R S L LK D C+ ++A P R ++ V PPG P I + +T + + C S G P L+W ++R Y T+ +S Y T T A +K A+ C A+N V+S V+L +L P V + G +R G+ +TCTT P A IKW+ A + +SP E W + N T ++ NK+ ++V S +++ Y P P I GY G+ + ++ C + GGNP L W+++ K I S + + + +I I +++DN+ +Y C+A S S PL TL V F P T+ ++ + E+ G + C + SNPPA + W + P++ N + G +GG++ F + + + + C+ N + + + ++V + P+F + EG+S ++L+ ANP Y W ++ T + + H I L D Y+ N +G + + I + + Y I K + I+ ++A L C+V+ P KW + +G + ++ S + N TS L I D R DVG +RCV +N + + I L +F P+I P +AA GE L C + P P+ W + + ++ KY + + + Y + L + + D+G+Y C A N G + + TS PD PL + ++N + + ++W GFDGG +++VR++ + Y+Y D + K+T I GL+ +T YL S+ S N
Sbjct: 72 QKFRTTPHDL-QVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGP-ARLNSAIRANAKLTVISPPASIEI--KGYSHNSKV----EVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDM-PMRATVQLSVLY----PPGPPYIEGYSAGET--LRRGQTVELMCRSR-GGNPPAQLIWYKNGSQIRMAYR------TSGRLSENIY-----------TFTAEAGDNK---ARFRCEASN-VMSQNPLKAEVELSVLFAPTHVTVM---GPTEARV---------------GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSP-EGGW----TTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINS--VIRAADKSVSAEITILANVSDNQAQYRCEA-SNSATEIPL-FQSTTLSVHFAPETVKIR-IEPEELRPGMEATIICDSSSSNPPAKLSWWKDGI----------PIEGINN---------TSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEAL---QRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTI---KSVSENIVVNPGEDAMLSCTVEGKPLT-EEHVKWER--------------------VGYDMTVKTSTTFANG---------------------------------TSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYR-MADREQYKYIDGLPNSHKLT----IGGLRMNTLYLFSVMSWN 1042
BLAST of NPHS1-6 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0111043) HSP 1 Score: 248.825 bits (634), Expect = 4.018e-66 Identity = 276/1151 (23.98%), Postives = 460/1151 (39.97%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSR----SRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQ--STAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNP---AINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEII---RQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYP 3731
Q F+ P + +V +G+ A +RC V N G VQW LG+ IPG P Y V+G +G + L ISN ++ D Y+CQV P + + +R KL V+ P +I KG+ N ++++ + C+ N +P + W+ N + + +EE R S L LK D C+ ++A P R ++ V PPG P I + +T + + C S G P L+W ++R Y T+ +S Y T T A +K A+ C A+N V+S V+L +L P V + G +R G+ +TCTT P A IKW+ A + +SP E W + N T ++ NK+ ++V S +++ Y P P I GY G+ + ++ C + GGNP L W+++ K I S + + + +I I +++DN+ +Y C+A S S PL TL V F P T+ ++ + E+ G + C + SNPPA + W + P++ N + G +GG++ F + + + + C+ N + + + ++V + P+F + EG+S ++L+ ANP Y W ++ T + + H I L D Y+ N +G + + I + + Y I K + I+ ++A L C+V+ P KW + +G + ++ S + N TS L I D R DVG +RCV +N + + I L +F P+I P +AA GE L C + P P+ W + + ++ KY + + + Y + L + + D+G+Y C A N G + + TS PD PL + ++N + + ++W GFDGG +++VR++ + Y+Y D + K+T I GL+ +T YL S+ S N +S T KE P++P
Sbjct: 72 QKFRTTPHDL-QVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPA-RLNSAIRANAKLTVISPPASIEI--KGYSHNSKV----EVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDM-PMRATVQLSVLY----PPGPPYIEGYSAGET--LRRGQTVELMCRSR-GGNPPAQLIWYKNGSQIRMAYR------TSGRLSENIY-----------TFTAEAGDNK---ARFRCEASN-VMSQNPLKAEVELSVLFAPTHVTVM---GPTEARV---------------GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSP-EGGW----TTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINS--VIRAADKSVSAEITILANVSDNQAQYRCEA-SNSATEIPL-FQSTTLSVHFAPETVKIR-IEPEELRPGMEATIICDSSSSNPPAKLSWWKDGI----------PIEGINN---------TSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEAL---QRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTI---KSVSENIVVNPGEDAMLSCTVEGKPLT-EEHVKWER--------------------VGYDMTVKTSTTFANG---------------------------------TSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYR-MADREQYKYIDGLPNSHKLT----IGGLRMNTLYLFSVMSWNELGQSSYLPDLARAET------KEAPPPSHP 1067
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 228.794 bits (582), Expect = 4.737e-60 Identity = 265/1143 (23.18%), Postives = 458/1143 (40.07%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQP--NGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNEN---SALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVI-SYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRV-KLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPI--QSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFS---KTIMTNTMSA 3695
Q F+ P+ V V G+ A L+C V G VQW +LLG R++PG P Y + G KG++ L+I +VT++D G YECQV P++ R L V + QP + +P G+Q + TC++ + +P + F + + ++E+ S ++L + + ++++ D + CR N A+ R+L T + + ++ PP P I+ + + V + + S G P +L W K E+ + T+ D+ + S L + H A L C + N V S S L +L P +V + G E V GEE ++C+T I+W +N ++ E ++ S + K++++ + L C+A++ G + R +L + Y P+K + + + C + GGNP +L W +++K + S ++ + G + L +I+ PS DN Y C + S K+ + S + VLFP +++ + + E+ G+ L L C AG SNP A + W L PV +L +FGG S + + C+ F+ E + ++V F P+F + E ++ TL + ANP W KL ++ + S L I N D +Y + N G ++ + L++ Y P + I A+L C +NP M + W +W G E+ +S+ + T +L IY+V R+ G+Y+C +NGI +L F P + P++ K A G+ + ++ C + P + W + + N +Y T + + L ++N+ D+ + C A N G DS I++ T+ PD P +++++ Q+ + + W+ GFDGG Q F+VR++ S Y Y DV + + +TGL T Y S+N+ NS G S ++ K +M T A
Sbjct: 27 QAFKTEPRNV-TVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTV-----------TCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASP-------RALETGMTMKVYFPPQAPMIMGLENEE----VKAGSFLKVVCMSYGGNPLATLHWT----KNGEVLS------TSWDVD-------FVSRRASSVLKMEVKPEDNH-AVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLL--------------GSFEAV----EGEEINLSCSTSSSNPPVHIRWWLGFK-ELNRTEVTFAEG---DNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPS--DNLATYRCDS---SNKAKKVLSTQTKFRVLFPAVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISWSLG------------PV-------ILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEG---VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSM-FSW----------------------------------------------KWMGEGERDELGE------ESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDL-GNPRYE-ETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSAS-YMYVDVFPPTATV---YTVTGLNPSTMYNFSVNALNSI-GESSYADNNKILMVTTADA 1018
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 228.794 bits (582), Expect = 5.219e-60 Identity = 265/1143 (23.18%), Postives = 458/1143 (40.07%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQP--NGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNEN---SALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVI-SYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRV-KLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPI--QSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFS---KTIMTNTMSA 3695
Q F+ P+ V V G+ A L+C V G VQW +LLG R++PG P Y + G KG++ L+I +VT++D G YECQV P++ R L V + QP + +P G+Q + TC++ + +P + F + + ++E+ S ++L + + ++++ D + CR N A+ R+L T + + ++ PP P I+ + + V + + S G P +L W K E+ + T+ D+ + S L + H A L C + N V S S L +L P +V + G E V GEE ++C+T I+W +N ++ E ++ S + K++++ + L C+A++ G + R +L + Y P+K + + + C + GGNP +L W +++K + S ++ + G + L +I+ PS DN Y C + S K+ + S + VLFP +++ + + E+ G+ L L C AG SNP A + W L PV +L +FGG S + + C+ F+ E + ++V F P+F + E ++ TL + ANP W KL ++ + S L I N D +Y + N G ++ + L++ Y P + I A+L C +NP M + W +W G E+ +S+ + T +L IY+V R+ G+Y+C +NGI +L F P + P++ K A G+ + ++ C + P + W + + N +Y T + + L ++N+ D+ + C A N G DS I++ T+ PD P +++++ Q+ + + W+ GFDGG Q F+VR++ S Y Y DV + + +TGL T Y S+N+ NS G S ++ K +M T A
Sbjct: 72 QAFKTEPRNV-TVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTV-----------TCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASP-------RALETGMTMKVYFPPQAPMIMGLENEE----VKAGSFLKVVCMSYGGNPLATLHWT----KNGEVLS------TSWDVD-------FVSRRASSVLKMEVKPEDNH-AVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLL--------------GSFEAV----EGEEINLSCSTSSSNPPVHIRWWLGFK-ELNRTEVTFAEG---DNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPS--DNLATYRCDS---SNKAKKVLSTQTKFRVLFPAVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISWSLG------------PV-------ILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEG---VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSM-FSW----------------------------------------------KWMGEGERDELGE------ESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDL-GNPRYE-ETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSAS-YMYVDVFPPTATV---YTVTGLNPSTMYNFSVNALNSI-GESSYADNNKILMVTTADA 1063
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 165.622 bits (418), Expect = 1.190e-40 Identity = 213/967 (22.03%), Postives = 373/967 (38.57%), Query Frame = 3
Query: 828 SRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVI-SYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRV-KLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPI--QSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFS---KTIMTNTMSA 3695
+ + ++++ D + CR N A+ R+L T + + ++ PP P I+ + + V + + S G P +L W K E+ + T+ D+ + S L + H A L C + N V S S L +L P +V + G E V GEE ++C+T I+W +N ++ E ++ S + K++++ + L C+A++ G + R +L + Y P+K + + + C + GGNP +L W +++K + S ++ + G + L +I+ PS DN Y C + S K+ + S + VLFP +++ + + E+ G+ L L C AG SNP A + W L PV +L +FGG S + + C+ F+ E + ++V F P+F + E ++ TL + ANP W KL ++ + S L I N D +Y + N G ++ + L++ Y P + I A+L C +NP M + W +W G E+ +S+ + T +L IY+V R+ G+Y+C +NGI +L F P + P++ K A G+ + ++ C + P + W + + N +Y T + + L ++N+ D+ + C A N G DS I++ T+ PD P +++++ Q+ + + W+ GFDGG Q F+VR++ S Y Y DV + + +TGL T Y S+N+ NS G S ++ K +M T A
Sbjct: 64 THAEVTIRARSSDDTRRLTCRAKNPASP-------RALETGMTMKVYFPPQAPMIMGLENEE----VKAGSFLKVVCMSYGGNPLATLHWT----KNGEVLS------TSWDVD-------FVSRRASSVLKMEVKPEDNH-AVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLL--------------GSFEAV----EGEEINLSCSTSSSNPPVHIRWWLGFK-ELNRTEVTFAEG---DNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPS--DNLATYRCDS---SNKAKKVLSTQTKFRVLFPAVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISWSLG------------PV-------ILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEG---VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSM-FSW----------------------------------------------KWMGEGERDELGE------ESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDL-GNPRYE-ETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSAS-YMYVDVFPPTATV---YTVTGLNPSTMYNFSVNALNSI-GESSYADNNKILMVTTADA 891 HSP 2 Score: 52.373 bits (124), Expect = 6.145e-6 Identity = 65/282 (23.05%), Postives = 117/282 (41.49%), Query Frame = 3
Query: 1695 EKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLK-----IIITPSINDNKVKYFCQAKSISLKSSPLNSDE-VTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEG---IINYNFYIEEGQSQTLNLE-PIANPPIKDYTWFRNSTKLSRKNV 2510
E+P ++G + +TC P E+ FRD + + S G+Q L+ + I +D+ + C+AK+ +SP + +T+ V FPP + L EV+ G LK+ C + NP A + W N G+++S T+ + + +S +S+ + E+ P +D+ + C+ N QV R + + T + FE + +F EG+ L+ +NPP+ W+ +L+R V
Sbjct: 13 EEPWVEG----------EQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKN---PASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKN--GEVLS--------TSWDVDFVSRRASSVLK-----------MEVKP--EDNHAVLQCQSIN-------QVTRSPMSLTRTLTVLFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVH-IRWWLGFKELNRTEV 250
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322]) HSP 1 Score: 84.3445 bits (207), Expect = 1.760e-15 Identity = 147/632 (23.26%), Postives = 226/632 (35.76%), Query Frame = 3
Query: 1641 SGIKSVQSRVKLHITYSPEKPTIQGYNVGKP----VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFE---GIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFER-KEIIRQNAELECSVQSNPANPHMSYKWTK--------------LVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKN--------------EKPKPYS-----------KAEWKGTPEKSNFKYFSKCMVKSQFEM--------TSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILS-LPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSR 3368
+G S+Q V++ + P+I+G + P V +N + C + G+P P++ W +DS+P+ + + N +TL+ I+ + D +Y C A++++ + L V P +G++ + V N + L C A PP +RW + RRPVQ + N ++ GG +Q + D Y C NP GE +K I + V P G + ++ G+S L E A PP TW++N ++ I L ++ + D Y N G+ +LNI P + G ER E I C P S W K ++ G IS S L + T C S K KN E P S +AE TPE ++ M S+ S L + V +D G Y CV N G+ + L P I P + L +++ C P P++ W VS+ R + V L+I Q +D GSY C A N G D+R
Sbjct: 2839 AGEDSIQYDVRVLL-----PPSIRGADGELPDEVTVLVNKTMLLECQ-VDGSPTPKISWVKDSQPLTQDNTHRLLSNGRTLQ-ILNAQVTDTG-RYVCVAQNLAGTA----EKSFNLHVHVSPTIVGIREENVTVVLN-NFVSLNCEA-TGLPPPTLRWFKD----------RRPVQASTNALIMP-----------GGRTLQI---LKAKMSDGGKY-SCVAVNP-AGEA----QKLIYLTVYVPPSIRHNSGDLPVVLNVQVGKSVMLECESNAVPP-PVITWYKNGRPIAETANLRILADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFHLNIHVPPRLDGPAEERVVETISNPVTFACDATGIPPP---SLTWLKNGRVIENSESLEMHILSGGSKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTPTPE---IQWLKDGMTISRTAQKNIKISPDGSTLTVTAVHTSDSGKYTCVATNQA-GEEDRIFNLNVYVPPLIQGNGPAAKELTTVLDSSINIECVASGSPPPQLNWLKNGLPLPVSSQ-------IRLLSAGQV--LRIARAQVSDGGSYTCVASNRAGVDNR 3409 HSP 2 Score: 82.0333 bits (201), Expect = 7.278e-15 Identity = 237/1090 (21.74%), Postives = 390/1090 (35.78%), Query Frame = 3
Query: 459 GRPTYRVVG-SVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNST----------IKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL-SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQF--EGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFE--RKEI---IRQNAELECSVQSNP---------ANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS-----------------------------KAEWKGTPEKSNFKYFSKCMVKSQFEMT-----SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA-GNLGE--------EVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTP-------DVPLEIQV-VNKS---QSQIE------ISWIQ 3464
GRP G +++ H LEI V DAG Y+C V T + + P P I KG ++ + VR E +G P W + + + ++ L+ R LS +++ D C N E H++ E R ++ PP M ++ ++ + C+S PA L WR KD P Y + +S ++ S L I + ++ G + C ATN K Y L++ I SD +SS T+ + GL T+ C + I P IWK+D S + + ++I ++ D S C A S S L + P + + V + + C G P P + W +D +P+ + AQ + + L+ I+ + D +Y C A +++ ++ + + V PP IG + V + L C A PPA+ K GQ +S ++SS I+G G L+ + + D Y C V N GEE RK + V P EG + + ++E Q+ L E N P+ + TW ++ L+ + ++ L I ++ D Y+ + N G+ LN+ P I G E +E+ + L C QS P P S + +++PG I ++ A C + + KN + K + + E + P + Y ++K+ +T ++ I +D G Y CV N I G+ K ++ + P S F + GN GE +SL+C + P P +TW+ + +++N K I G V L+I Q D G Y C A N G DS + +V P ++P E+ V VNK+ + Q++ ISW++
Sbjct: 1951 GRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKC----VAINVAGSTELTYSLQVYVP--PTISSKG------GMVTVVVNDPVRLE---CEASGVPVPSLTWLKEGSPVSSFSDG--IQVLSGGRV-LSFISAQVSDAGHYTCVAVNAGGEQHREYELR---------VYVPPNI-----MGEEVNNTVLMGQSVQLHCQSDAIPPPA--LSWR-------------------KDGRPLYRKPGLSVSEDGSFLKIISAQVQDTG-RYTCEATN----VAGKTEKHYNLNVWVPPSIRGSD-EVSSLTVIEGGL------------ITLVC---------------------ESSGIPPPSLIWKKDGSELKGDSRVRVLSGGRQLQISSAEKADASSYTCLASSAAGSAIKEYNLQVYVRPSISS--SAQDEETVTRGGDITLRCEA-DGVPRPAVSWMKDGRPLSTGRKAQILNEGRLLR-ILEAQVADTG-RYTCIAINVAGQA----DRKYDVNVHVPPTIIGQTHMPENISVVVKNPVVLTCEASGMPPPAITWLKD---GQPIS----------------TSSSVRVISGGRGLRLMHA------ASSDAGRYT-CIVSN-SAGEE----RKNFDLYVLVPPSIVNEGTVE-DVKVKERQNAILACEVTGN-PVPEITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASNSAGDRSRHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLVCEAQSYPPAIITWLKDGTPFESSRNVRVLPGGRTLQILNAKEEDAGRYTCVATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVNNTLTLECEAQAIPTPTLVWYKDGQILKADGHLTITANGRIVQIKHTQVSDTGRYTCVATN-IAGEDEKDFDVNIQVPP---SFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWY--KDGHLLTSNDKVLI-----LPGGRV--LQIPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPPSIRGADGELPDEVTVLVNKTMLLECQVDGSPTPKISWVK 2893 HSP 3 Score: 72.4034 bits (176), Expect = 6.547e-12 Identity = 102/395 (25.82%), Postives = 161/395 (40.76%), Query Frame = 3
Query: 1581 TIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSG-----AEVENGKLLKLQCTAGESN--PPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQ-SYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL--SRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKF--ERKEIIRQNAELECSVQSNP 2729
T++I + D C A + + + LH+ SP I+ NV V LN+ + C G P P L+WF+D +P+Q+S A + +TL+ I+ ++D KY C A + + ++ L + L V PP ++ SG V+ GK + L+C ESN PP V+ W N RP+ T N +L+ G ++Q E+ D YV C+ N QV K +N+ P+ +G T + PP TW +N + S I + +KL I ++ D YT V N G++Q +L I P IIG + E ++ + +L C + P
Sbjct: 2912 TLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENV--TVVLNNFVSLNCEATG-LPPPTLRWFKDRRPVQASTNALIMPGGRTLQ-ILKAKMSDGG-KYSCVAVNPAGEAQKL----IYLTVYVPP---SIRHNSGDLPVVLNVQVGKSVMLEC---ESNAVPPPVITWYKN----------GRPIAETANLRILA-----------DGQMLQLKETEV----SDTGQYV-CKATN----VAGQV-DKNFHLNIHVPPRLDGPAEERVVETISNPVTFACDATGIPP-PSLTWLKNGRVIENSESLEMHILSGGSKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTP 3259 HSP 4 Score: 68.5514 bits (166), Expect = 1.037e-10 Identity = 86/372 (23.12%), Postives = 133/372 (35.75%), Query Frame = 3
Query: 2463 DYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPAN----PH-----MSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQS---------------------LPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMT-----SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTW--------FFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAEN--GKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQ 3443
+ TW R +R + T+ L I+ P D+ YT + N+ G S +YLN+ P++ D + Q + CS + P+ H M ++ P G I ++ L A T GC S +P S K + G P Y +K ++ L I G Y CV N G I L PQ P S + ++G ++L CY + HP P+++W F R+ S + R G + I NL D +YVC+A+N G+ K+ V+G +P + L VV+ + Q
Sbjct: 544 NLTWQRGDVD-ARLDHRIRVTTNLSLEIQRVTPDDVGWYTCIAANEGGVSAARVYLNVQEPPVVTVDPRNQTFQTGQEIWIRCSAKGYPSPMVVWTHNDMFIMGSSRHRMTP-DGTLIIKNTGLKDAGTYGCLASNVAGTDSQTAIVSYIESPQVTVPHSDLLIGLGQKTVMECRVTGVPHPDIMWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQYTCVAVNSA-GSAQGYITLDVGSAPQFTKKP--SDLSADIGTNITLPCYAQGHPKPQLSWRREDNIALFTQRHGPSTVTH--------RMDGGLF-----ITNLWVEDEATYVCEAQNHFGRIQVKAKVTVTGLVSPLIALSPTVVSVIEDQ 897 HSP 5 Score: 53.5286 bits (127), Expect = 2.958e-6 Identity = 119/539 (22.08%), Postives = 205/539 (38.03%), Query Frame = 3
Query: 510 LEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNE-NSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTT--DPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVK--LNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPP-MTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRW 2105
L ++ V + D GIY C+ V G+E + + P ++ F + + N + F C + G P+ +WF N + + NE ++LE+ + L L + D C N A + + + ++ PP I D V++ + C+ + G P + W Y + E + D+ Q + K K + L + + + C A N + + L +L +P ++ +V LS+ LN+ ++ + C DP+ TI+W P D E R +KIK + DQ+ C A + L I + P P+I+G NV V L + + C + G P P + W+++ + I SS AQ V + LK I+ ++D +Y C+ S++ + + V L V PP +T G + +V K L L+C A E +PP + W
Sbjct: 1219 LTLNQVALTDEGIYVCRAVNIAGKEE-----TAIQLHVQVPPVVEVSEPPFNSPLQERVANQ-----QIAFPCPA-KGTPEPVIKWFRNGHELTGNEPGVSILED----GTLLILASVSPLDNGEYTCMAVNDAGTTEKKYQLK---------VNVPP---DIRDNGLLSNVSVVINQPTNLVCDVT--GTPVPVITW----------YKDGVEVVPSSDV------QILQKGKTLKLLK----AAVQDAGRYICKAIN-IAGSRERDFYLDVL-VPPTIIGTVGSRDLSAV------LNQEIV---------LECKVKGDPF---PTIQWYKDRKPVFLGD---------PNIEVLNRGQQLKIKSARLGDQARYQCSATNAAGKQSKDFNLSI-FVP--PSIKGGNVSSEVTVLLGNLVTLECE-VRGVPLPAVTWYKNGEVILSSRQAQYVDRGQFLK-ILRAQVSDAG-QYTCRVTSVAGTAEKV----VELDVYVPPSITAGSDGPTDMKVVLNKSLILECEA-EGHPPPSLTW 1668
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322]) HSP 1 Score: 83.9593 bits (206), Expect = 1.790e-15 Identity = 147/632 (23.26%), Postives = 226/632 (35.76%), Query Frame = 3
Query: 1641 SGIKSVQSRVKLHITYSPEKPTIQGYNVGKP----VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFE---GIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFER-KEIIRQNAELECSVQSNPANPHMSYKWTK--------------LVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKN--------------EKPKPYS-----------KAEWKGTPEKSNFKYFSKCMVKSQFEM--------TSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILS-LPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSR 3368
+G S+Q V++ + P+I+G + P V +N + C + G+P P++ W +DS+P+ + + N +TL+ I+ + D +Y C A++++ + L V P +G++ + V N + L C A PP +RW + RRPVQ + N ++ GG +Q + D Y C NP GE +K I + V P G + ++ G+S L E A PP TW++N ++ I L ++ + D Y N G+ +LNI P + G ER E I C P S W K ++ G IS S L + T C S K KN E P S +AE TPE ++ M S+ S L + V +D G Y CV N G+ + L P I P + L +++ C P P++ W VS+ R + V L+I Q +D GSY C A N G D+R
Sbjct: 2838 AGEDSIQYDVRVLL-----PPSIRGADGELPDEVTVLVNKTMLLECQ-VDGSPTPKISWVKDSQPLTQDNTHRLLSNGRTLQ-ILNAQVTDTG-RYVCVAQNLAGTA----EKSFNLHVHVSPTIVGIREENVTVVLN-NFVSLNCEA-TGLPPPTLRWFKD----------RRPVQASTNALIMP-----------GGRTLQI---LKAKMSDGGKY-SCVAVNP-AGEA----QKLIYLTVYVPPSIRHNSGDLPVVLNVQVGKSVMLECESNAVPP-PVITWYKNGRPIAETANLRILADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFHLNIHVPPRLDGPAEERVVETISNPVTFACDATGIPPP---SLTWLKNGRVIENSESLEMHILSGGSKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTPTPE---IQWLKDGMTISRTAQKNIKISPDGSTLTVTAVHTSDSGKYTCVATNQA-GEEDRIFNLNVYVPPLIQGNGPAAKELTTVLDSSINIECVASGSPPPQLNWLKNGLPLPVSSQ-------IRLLSAGQV--LRIARAQVSDGGSYTCVASNRAGVDNR 3408 HSP 2 Score: 82.0333 bits (201), Expect = 7.464e-15 Identity = 237/1090 (21.74%), Postives = 390/1090 (35.78%), Query Frame = 3
Query: 459 GRPTYRVVG-SVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNST----------IKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL-SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQF--EGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFE--RKEI---IRQNAELECSVQSNP---------ANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS-----------------------------KAEWKGTPEKSNFKYFSKCMVKSQFEMT-----SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA-GNLGE--------EVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTP-------DVPLEIQV-VNKS---QSQIE------ISWIQ 3464
GRP G +++ H LEI V DAG Y+C V T + + P P I KG ++ + VR E +G P W + + + ++ L+ R LS +++ D C N E H++ E R ++ PP M ++ ++ + C+S PA L WR KD P Y + +S ++ S L I + ++ G + C ATN K Y L++ I SD +SS T+ + GL T+ C + I P IWK+D S + + ++I ++ D S C A S S L + P + + V + + C G P P + W +D +P+ + AQ + + L+ I+ + D +Y C A +++ ++ + + V PP IG + V + L C A PPA+ K GQ +S ++SS I+G G L+ + + D Y C V N GEE RK + V P EG + + ++E Q+ L E N P+ + TW ++ L+ + ++ L I ++ D Y+ + N G+ LN+ P I G E +E+ + L C QS P P S + +++PG I ++ A C + + KN + K + + E + P + Y ++K+ +T ++ I +D G Y CV N I G+ K ++ + P S F + GN GE +SL+C + P P +TW+ + +++N K I G V L+I Q D G Y C A N G DS + +V P ++P E+ V VNK+ + Q++ ISW++
Sbjct: 1950 GRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKC----VAINVAGSTELTYSLQVYVP--PTISSKG------GMVTVVVNDPVRLE---CEASGVPVPSLTWLKEGSPVSSFSDG--IQVLSGGRV-LSFISAQVSDAGHYTCVAVNAGGEQHREYELR---------VYVPPNI-----MGEEVNNTVLMGQSVQLHCQSDAIPPPA--LSWR-------------------KDGRPLYRKPGLSVSEDGSFLKIISAQVQDTG-RYTCEATN----VAGKTEKHYNLNVWVPPSIRGSD-EVSSLTVIEGGL------------ITLVC---------------------ESSGIPPPSLIWKKDGSELKGDSRVRVLSGGRQLQISSAEKADASSYTCLASSAAGSAIKEYNLQVYVRPSISS--SAQDEETVTRGGDITLRCEA-DGVPRPAVSWMKDGRPLSTGRKAQILNEGRLLR-ILEAQVADTG-RYTCIAINVAGQA----DRKYDVNVHVPPTIIGQTHMPENISVVVKNPVVLTCEASGMPPPAITWLKD---GQPIS----------------TSSSVRVISGGRGLRLMHA------ASSDAGRYT-CIVSN-SAGEE----RKNFDLYVLVPPSIVNEGTVE-DVKVKERQNAILACEVTGN-PVPEITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASNSAGDRSRHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLVCEAQSYPPAIITWLKDGTPFESSRNVRVLPGGRTLQILNAKEEDAGRYTCVATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVNNTLTLECEAQAIPTPTLVWYKDGQILKADGHLTITANGRIVQIKHTQVSDTGRYTCVATN-IAGEDEKDFDVNIQVPP---SFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWY--KDGHLLTSNDKVLI-----LPGGRV--LQIPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPPSIRGADGELPDEVTVLVNKTMLLECQVDGSPTPKISWVK 2892 HSP 3 Score: 72.4034 bits (176), Expect = 6.492e-12 Identity = 102/395 (25.82%), Postives = 161/395 (40.76%), Query Frame = 3
Query: 1581 TIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSG-----AEVENGKLLKLQCTAGESN--PPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQ-SYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL--SRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKF--ERKEIIRQNAELECSVQSNP 2729
T++I + D C A + + + LH+ SP I+ NV V LN+ + C G P P L+WF+D +P+Q+S A + +TL+ I+ ++D KY C A + + ++ L + L V PP ++ SG V+ GK + L+C ESN PP V+ W N RP+ T N +L+ G ++Q E+ D YV C+ N QV K +N+ P+ +G T + PP TW +N + S I + +KL I ++ D YT V N G++Q +L I P IIG + E ++ + +L C + P
Sbjct: 2911 TLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENV--TVVLNNFVSLNCEATG-LPPPTLRWFKDRRPVQASTNALIMPGGRTLQ-ILKAKMSDGG-KYSCVAVNPAGEAQKL----IYLTVYVPP---SIRHNSGDLPVVLNVQVGKSVMLEC---ESNAVPPPVITWYKN----------GRPIAETANLRILA-----------DGQMLQLKETEV----SDTGQYV-CKATN----VAGQV-DKNFHLNIHVPPRLDGPAEERVVETISNPVTFACDATGIPP-PSLTWLKNGRVIENSESLEMHILSGGSKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTP 3258 HSP 4 Score: 68.5514 bits (166), Expect = 1.037e-10 Identity = 86/372 (23.12%), Postives = 133/372 (35.75%), Query Frame = 3
Query: 2463 DYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPAN----PH-----MSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQS---------------------LPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMT-----SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTW--------FFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAEN--GKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQ 3443
+ TW R +R + T+ L I+ P D+ YT + N+ G S +YLN+ P++ D + Q + CS + P+ H M ++ P G I ++ L A T GC S +P S K + G P Y +K ++ L I G Y CV N G I L PQ P S + ++G ++L CY + HP P+++W F R+ S + R G + I NL D +YVC+A+N G+ K+ V+G +P + L VV+ + Q
Sbjct: 543 NLTWQRGDVD-ARLDHRIRVTTNLSLEIQRVTPDDVGWYTCIAANEGGVSAARVYLNVQEPPVVTVDPRNQTFQTGQEIWIRCSAKGYPSPMVVWTHNDMFIMGSSRHRMTP-DGTLIIKNTGLKDAGTYGCLASNVAGTDSQTAIVSYIESPQVTVPHSDLLIGLGQKTVMECRVTGVPHPDIMWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQYTCVAVNSA-GSAQGYITLDVGSAPQFTKKP--SDLSADIGTNITLPCYAQGHPKPQLSWRREDNIALFTQRHGPSTVTH--------RMDGGLF-----ITNLWVEDEATYVCEAQNHFGRIQVKAKVTVTGLVSPLIALSPTVVSVIEDQ 896 HSP 5 Score: 53.5286 bits (127), Expect = 2.983e-6 Identity = 119/539 (22.08%), Postives = 205/539 (38.03%), Query Frame = 3
Query: 510 LEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNE-NSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTT--DPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVK--LNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPP-MTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRW 2105
L ++ V + D GIY C+ V G+E + + P ++ F + + N + F C + G P+ +WF N + + NE ++LE+ + L L + D C N A + + + ++ PP I D V++ + C+ + G P + W Y + E + D+ Q + K K + L + + + C A N + + L +L +P ++ +V LS+ LN+ ++ + C DP+ TI+W P D E R +KIK + DQ+ C A + L I + P P+I+G NV V L + + C + G P P + W+++ + I SS AQ V + LK I+ ++D +Y C+ S++ + + V L V PP +T G + +V K L L+C A E +PP + W
Sbjct: 1218 LTLNQVALTDEGIYVCRAVNIAGKEE-----TAIQLHVQVPPVVEVSEPPFNSPLQERVANQ-----QIAFPCPA-KGTPEPVIKWFRNGHELTGNEPGVSILED----GTLLILASVSPLDNGEYTCMAVNDAGTTEKKYQLK---------VNVPP---DIRDNGLLSNVSVVINQPTNLVCDVT--GTPVPVITW----------YKDGVEVVPSSDV------QILQKGKTLKLLK----AAVQDAGRYICKAIN-IAGSRERDFYLDVL-VPPTIIGTVGSRDLSAV------LNQEIV---------LECKVKGDPF---PTIQWYKDRKPVFLGD---------PNIEVLNRGQQLKIKSARLGDQARYQCSATNAAGKQSKDFNLSI-FVP--PSIKGGNVSSEVTVLLGNLVTLECE-VRGVPLPAVTWYKNGEVILSSRQAQYVDRGQFLK-ILRAQVSDAG-QYTCRVTSVAGTAEKV----VELDVYVPPSITAGSDGPTDMKVVLNKSLILECEA-EGHPPPSLTW 1667
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620]) HSP 1 Score: 154.451 bits (389), Expect = 5.161e-37 Identity = 205/840 (24.40%), Postives = 330/840 (39.29%), Query Frame = 3
Query: 300 IVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNE--NSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTI-PALTSKEHGAQLFCVATNPVI-SYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEK---TITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAW--SGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQ-SSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPI---ANPPIKDYTWFRNSTKLSRKNV--HSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPII--IGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLV 2768
+ + Q F+ P + V QGS A L C V + G VQW +LLG DRNIPG Y + G KGE+ L I + D Y CQV ++ R + VL+ P P ++ + + ++ V TC + + +P + + + N ++L + + +G ++ C N A Q P S + V PP P I P V + S SG P +L W K ++ + R+E + + ++ TL I PA ++ A + C A N V T++ L ++ +P +V I + +G EK T +C T I+W P +N ++ + + + S + ++ ++ L C+A+ + + + + V L + Y P+ I+ K + + +MTC + GGNP P L W +D+K + S I K + II PS DN Y C A +I +P + L V FP + + + S S E G + L C G SNP + + W + +LGR+P +GG S + + KD+ V CE F+ E K +N+ F P+F ++ + + L P+ ANPP +YTW KL R H + A L I N D Y + VN G++ I I L++ Y P I +GD + + +AE+ C+ +NPA M ++W LV
Sbjct: 14 VTAKQQVFRVQPDNI-TVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPT--FEEYAAGSRVTWVYGVEYTV----TCQATDAKPAAALTIKKSGLEVSGDSSSNPGSRDKLENTAMVAKVIPQSSDNGKLLTCLALNPAL---QTPITVSFTMNVLF----PPNAPIIEGY----IGPSVKAGETLKLICISLSGNPLATLQWS----KNDDVVSTRWET-----------DEVVRASRSHLTLNIKPA----DNMAVVSCEAVNHVTPETLKTSIILNVVFLPTEVTI---------------------LGSSSGPEKSNLTFSCFTAASNPPVQIRWWLGPKELVNT-IVTISDAVHGGKVTM---SNLTMEALREEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHSGKIVTKEITIITVPS--DNMATYRCNASNIG--KTPALTASTKLKVQFPAIDVNIIS-SAKEYRRGSTIILTCVTGSSNPASTISWVKDGEQLKAQDLGRKP-------------------SLYGGISSSSRVTLKAASKDNGRRVSCEAFSSVLNEAVNTFYK---LNILFPPEF---LDDQPAVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGA-LEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDT---EVDLGSDAEIACTADANPATDSM-FQWRWLV 756 HSP 2 Score: 56.9954 bits (136), Expect = 3.385e-7 Identity = 41/147 (27.89%), Postives = 63/147 (42.86%), Query Frame = 3
Query: 3072 LTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVR 3503
L +F P I SK A G+ +L C PS +W + S+ T + + L I N+ D+ + C A N G DS I++ S PD P ++V+ + + + + W GFDGG+ Q F+VR
Sbjct: 779 LFYQFKPDIHKGVHLSKVAVPGDGKSVAALVCKAEGIPSVAFSW--AKNGVSLDLKDPRYSEKTFHELSVHTSTLVISNVSAVKDYALFTCTATNTLGVDSFTIQLVSPSRPDPPSGLRVIGFTHNWVTLQWSAGFDGGAEQKFRVR 923
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: rps7 (ribosomal protein S7 [Source:Xenbase;Acc:XB-GENE-977326]) HSP 1 Score: 77.7962 bits (190), Expect = 8.624e-14 Identity = 67/238 (28.15%), Postives = 104/238 (43.70%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWF-----LNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNII 1046
V G +L C + +G + W + +G R++ G P Y V G + GEH L I ++D +YECQ +Q + P +L VLV PD P I G VI SI+ TC +DN +P W LN L++D + +++ S L + +S ++G I CR +N+A ++ V I I PP V+ P V++DN++
Sbjct: 17 VVAGQPVSLLCAIPGYHGLLLWIKDGLAIGVGRDLSGYPRYSVGGDHLSGEHHLMIQRAELQDDAMYECQAIQAAIRSRP-----ARLTVLVPPDDPVI-------TGGPVI--SIRAGDPLNLTCHADNAKPAATITWMKNGEVLNGTLLRDGKRESII-------STLFVSSSDIENGQTITCRATNKAVPGGKE-------ATVTINIQHPPLVNLTVEPQP------VLEDNVV 220 HSP 2 Score: 53.1434 bits (126), Expect = 4.001e-6 Identity = 68/387 (17.57%), Postives = 146/387 (37.73%), Query Frame = 3
Query: 1611 DQSFLDCQAWSGIKSVQSR-VKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDE-VTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDR---GESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKW 2756
D + +CQA +++SR +L + P+ P I G V ++ +TC P + W ++ + + + + + ++ S +N C+A + K+ P + VT+ + PP+ + L ++ V ++K C A + PAV +++ + GQ++ E+ +T + + S + G + + ++Y F P E + + ++ G T + I NP + W + + + N ++ L ++N + D Y V R GE I++ +N P II + + + +++C ++S P +++ W
Sbjct: 79 DDAMYECQAIQA--AIRSRPARLTVLVPPDDPVITGGPV-ISIRAGDPLNLTCHADNAKPAATITWMKNGEVLNGTLLRDGKRESIISTLFVSSSDIENGQTITCRATN---KAVPGGKEATVTINIQHPPL-VNL-TVEPQPVLEDNVVKFHCAAKAN--PAVTQYRWAKGGQILKEVTDETYETVVDFSFFTEPVSCEVTNALGSTNVSRTVDVY--------------FGPRMSSEPE----------------------SITVDLGSDATFHCAWIGNPSLT-IVWMKRGSGVVLSNENT-------LTLKNVRQEDAGKYVCRAVVPRVGAGERDISLTVN---GPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSW 408
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: rps7 (ribosomal protein S7 [Source:Xenbase;Acc:XB-GENE-977326]) HSP 1 Score: 73.559 bits (179), Expect = 2.136e-12 Identity = 66/243 (27.16%), Postives = 103/243 (42.39%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNN----------NLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNII 1046
V G +L C + +G + W + +G R++ G P Y V G + GEH L I ++D +YECQ +Q + P +L VLV PD P I G VI SI+ TC +DN +P W N L++D + +++ S L + +S ++G I CR +N+A ++ V I I PP V+ P V++DN++
Sbjct: 25 VVAGQPVSLLCAIPGYHGLLLWIKDGLAIGVGRDLSGYPRYSVGGDHLSGEHHLMIQRAELQDDAMYECQAIQAAIRSRP-----ARLTVLVPPDDPVI-------TGGPVI--SIRAGDPLNLTCHADNAKPAATITWMKNGEVLNGATYYKTLLRDGKRESII-------STLFVSSSDIENGQTITCRATNKAVPGGKE-------ATVTINIQHPPLVNLTVEPQP------VLEDNVV 233
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: rps7 (ribosomal protein S7 [Source:Xenbase;Acc:XB-GENE-977326]) HSP 1 Score: 73.1738 bits (178), Expect = 2.210e-12 Identity = 66/243 (27.16%), Postives = 103/243 (42.39%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNN----------NLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNII 1046
V G +L C + +G + W + +G R++ G P Y V G + GEH L I ++D +YECQ +Q + P +L VLV PD P I G VI SI+ TC +DN +P W N L++D + +++ S L + +S ++G I CR +N+A ++ V I I PP V+ P V++DN++
Sbjct: 17 VVAGQPVSLLCAIPGYHGLLLWIKDGLAIGVGRDLSGYPRYSVGGDHLSGEHHLMIQRAELQDDAMYECQAIQAAIRSRP-----ARLTVLVPPDDPVI-------TGGPVI--SIRAGDPLNLTCHADNAKPAATITWMKNGEVLNGATYYKTLLRDGKRESII-------STLFVSSSDIENGQTITCRATNKAVPGGKE-------ATVTINIQHPPLVNLTVEPQP------VLEDNVV 225
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: rps7 (ribosomal protein S7 [Source:Xenbase;Acc:XB-GENE-977326]) HSP 1 Score: 73.1738 bits (178), Expect = 3.075e-12 Identity = 66/243 (27.16%), Postives = 103/243 (42.39%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQ-VQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNN----------NLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNII 1046
V G +L C + +G + W + +G R++ G P Y V G + GEH L I ++D +YECQ +Q + P +L VLV PD P I G VI SI+ TC +DN +P W N L++D + +++ S L + +S ++G I CR +N+A ++ V I I PP V+ P V++DN++
Sbjct: 30 VVAGQPVSLLCAIPGYHGLLLWIKDGLAIGVGRDLSGYPRYSVGGDHLSGEHHLMIQRAELQDDAMYECQAIQAAIRSRP-----ARLTVLVPPDDPVI-------TGGPVI--SIRAGDPLNLTCHADNAKPAATITWMKNGEVLNGATYYKTLLRDGKRESII-------STLFVSSSDIENGQTITCRATNKAVPGGKE-------ATVTINIQHPPLVNLTVEPQP------VLEDNVV 238
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 227.254 bits (578), Expect = 1.557e-59 Identity = 274/1167 (23.48%), Postives = 454/1167 (38.90%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD-----NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVIS-YQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRN-STIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQ-----QVGN--------QKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRI----QINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTG-FYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE-KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTN--TMSADNEKENNQPTYPIIQ 3740
V +GS L C VR VQW +LLG + IPG P Y + G KGE L I + D YECQV G+ + V+L P++ P + Q+ TCVS + +P + ++D NE S E+L ++ + + +G ++ C SN A P + S + + PPG P ++D +N+ + C + G P +L W V + + Q ++ + +V T+ ++HGA+L C + N V + Q ++ L + P+ V I +G +EN + T+ C T ++W + D E + + S + + + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W +DS+P+ +Q Q+G+ + L +II P DN K+ C+A +S + L+V FPP + + + S A + G L L C + SNPP V L++ G+ + ++ +P + F GS + S +DH V C + + LR+ + ++NV + P+F G + +E+GQ+ L + ANP + + W +LS H I S L + N D Y L N G ++ + L++ Y P I K + + + ++ C+V +NP P M + W +L G+ +++ D M K T L I + G Y+C+V+NG+ + L F PQ+ +K AAG+ +L C R P+ TW N R ++ G + + L I N+ D+ + C A N GSD I++ S PD PL ++VV+ S + + W GFDGG Q FQ+R++ +SP LY DV +F +TGLK T Y I + + N+ + K I + T D E+ P Q
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV---GRSELGPELVSPSVILSILVSPKVLQ--LTPEAGSTVTWVAGQEYVV--TCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSE--EKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILF----PPGPP-VIDWPGLNEGHVRAGENLELPCIAR-GGNPPATLQWLKNGKPVSIAWGTE-------------HAQAVAHSVLVMTVR-----PEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI-----------LGSTSQSEN-------KNVTLCCLTKSSRPRVLLRWWLGGRQLLPTD-----ETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPP--DNLAKFSCKAGQLSASTQ--------LVVQFPPTNLTILANSSA-LRPGDALNLTCVSISSNPP--VNLSLDKEGERLDDVAAKPQ-----------------SAPFKGSAASRSVFLRVSSRDHGHRVTCRAHS-------EALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRI-LSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEM-FSWERL--GEDEEELNLDD-----------------------------------------------MEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTW---TKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYM--DVLPAQAT---TFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQ 1067
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 225.713 bits (574), Expect = 3.751e-59 Identity = 274/1166 (23.50%), Postives = 455/1166 (39.02%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD-----NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVIS-YQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQ-----QVGN--------QKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRI----QINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTG-FYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE-KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTN--TMSADNEKENNQPTYPIIQ 3740
V +GS L C VR VQW +LLG + IPG P Y + G KGE L I + D YECQV G+ + V+L P++ P + Q+ TCVS + +P + ++D NE S E+L ++ + + +G ++ C SN A P + S + + PPG P ++D +N+ + C + G P +L W V + + Q ++ + +V T+ ++HGA+L C + N V + Q ++ L + P+ V I +G +EN + T+ C T ++W + D ++ + S S + + + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W +DS+P+ +Q Q+G+ + L +II P DN K+ C+A +S + L+V FPP + + + S A + G L L C + SNPP V L++ G+ + ++ +P + F GS + S +DH V C + + LR+ + ++NV + P+F G + +E+GQ+ L + ANP + + W +LS H I S L + N D Y L N G ++ + L++ Y P I K + + + ++ C+V +NP P M + W +L G+ +++ D M K T L I + G Y+C+V+NG+ + L F PQ+ +K AAG+ +L C R P+ TW N R ++ G + + L I N+ D+ + C A N GSD I++ S PD PL ++VV+ S + + W GFDGG Q FQ+R++ +SP LY DV +F +TGLK T Y I + + N+ + K I + T D E+ P Q
Sbjct: 43 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV---GRSELGPELVSPSVILSILVSPKVLQ--LTPEAGSTVTWVAGQEYVV--TCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSE--EKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILF----PPGPP-VIDWPGLNEGHVRAGENLELPCIAR-GGNPPATLQWLKNGKPVSIAWGTE-------------HAQAVAHSVLVMTVR-----PEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI-----------LGSTSQSEN-------KNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETV-MDGLHGGHISM---SNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPP--DNLAKFSCKAGQLSASTQ--------LVVQFPPTNLTILANSSA-LRPGDALNLTCVSISSNPP--VNLSLDKEGERLDDVAAKPQ-----------------SAPFKGSAASRSVFLRVSSRDHGHRVTCRAHS-------EALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRI-LSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEM-FSWERL--GEDEEELNLDD-----------------------------------------------MEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTW---TKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYM--DVLPAQAT---TFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQ 1053
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Hmcn1 (hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047]) HSP 1 Score: 88.9669 bits (219), Expect = 5.970e-17 Identity = 160/684 (23.39%), Postives = 264/684 (38.60%), Query Frame = 3
Query: 1734 VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMT--IGLKSLSGAEVENGKLLKLQCTAGESN--PPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN---VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKF--ERKEIIRQNAELECSVQSNPANPHMSYKWTK----LVPG-----KGYNDISSSDLVPAFT--IG---CNQSLPFSKE----------------YKNEKPKPYS----------KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDR---NDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGN------LGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKI--------EVSGTSTP-------DVPLEIQVVNKSQSQIEISWIQGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDT 3563
V +NH + C + G P P+L W ++ +PI+ + V +TL+ II I+D Y C I++ + + +V+L V PP G +SLS V G + L+C ESN PP V+ W N G+M+ + + N E+L+ G +L E+ D YV C N ++ K +NV P EG TL + PP TW +N + + VH +S +KL I + + NYT V N G++Q L I P + G + E ++ +N EL C+ P PH+ +W + +V G + D S+ ++ A T +G C + P +E K E K + KA P+ + K + S + S + + R +D+ +Y CV +N D +K ++ Q+ P A G G S+ C+ P+P M+W R ++ ++ ++ L+++ + D G Y C A N G S+ ++G+ P + PLE+ + +ISW++ DG +QT QV+ E ++ R +DT
Sbjct: 2975 VVVNHFISLNCE-VSGFPPPDLSWLKNEEPIKPNTNVLTVPGGRTLQ-IIRAKISDGG-DYTC----IAINQAGESKKKVSLTVHVPPSIKDHGSQSLSIVNVREGTSVSLEC---ESNAVPPPVITWSKN--GRMIPD--------STNVEILTG----------GQTLHIRRAEV----SDTGQYV-CRAINVAGRDD-----KNFHLNVYVPPTIEGPETEVIVETISNPVTLTCDATGIPP-PTITWLKNHKPIENSDPLEVHILS-GGSKLQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPPSVAGAEVPSEVSVLLGENVELVCNADGIP-TPHL--QWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGEEDRIFNLNVYVPPKIRGNKEEAEKLMALVDTSINIECKATGTPPPQINWLKNGLPLPISSHIRLLSAGQVVRIVRAQVSDIAVYTCVASNRAGVD-------SKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCFTDGTPAPSMSWL--RDGQPLAPDAHLTVSTQGM-------VLQLIKAETEDTGKYTCVATNEAGEVSKHFVLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMK--DGRPFLQTEQVQTLEGG--AILRVSSAQVEDT 3593 HSP 2 Score: 86.6557 bits (213), Expect = 3.340e-16 Identity = 139/621 (22.38%), Postives = 215/621 (34.62%), Query Frame = 3
Query: 1701 PTIQGYNVGKPVK--LNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDE--VTLIVLFPPMTIGLKSLSGAEVENGKLLKL-------QCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN-FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDK--FERKEIIRQNAELECSVQSNPA---------NPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMV-------------------KSQFEMT---------SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPL 3410
P++ G V V L N E+ C+ G P P LQW RD KPI + + + I ++ + KY C A ++P ++ L V PP + G + E KL+ L +C A PP + W N P+ + + LLSA G +++ D V+ V K + V P + + I +G S ++ P +W R+ L+ ++ST L + A+ D YT V N+ GE L + P I G + E I+ EL C PA P + + + + G +SS+ + C S P + K + + G E +F V + Q + T L I + D D Y CV +N I G + LT P I P+ L + ++L C P+P++TW R + V A S R +I GF L I + TD G Y+C A N G+D R+I++ P + +
Sbjct: 3244 PSVAGAEVPSEVSVLLGENVELVCNA-DGIPTPHLQWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVA------TNPAGEEDRIFNLNVYVPP------KIRGNKEEAEKLMALVDTSINIECKA-TGTPPPQINWLKNG----------LPLPISSHIRLLSA-----------GQVVRIV---------RAQVSDIAVYTCVASNRAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCF-TDGTPAPSMSWLRDGQPLAPDAHLTVSTQGMVLQLIKAETEDTGKYTCVATNEAGEVSKHFVLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMKDGRPFLQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTLECKSDAVPPPVIMWLKNREQLQATPRVRILSGGRYLQINNADLGDTANYTCVASN-IAGKTTREFNLTVNVPPSIGGGPQ--SLVTLLNKSIALECRAEGVPAPRITW---RKDGVVLAESHARYSI--LENGF----LHIESAHVTDTGRYLCMATNVAGTDRRRIDLQVHVPPSIAM 3807 HSP 3 Score: 65.0846 bits (157), Expect = 1.027e-9 Identity = 131/618 (21.20%), Postives = 228/618 (36.89%), Query Frame = 3
Query: 1641 SGIKSVQSRVKLHITYSPEKPTIQGYNV-GKP----VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMT-----IGLKSLSGAEVE--NGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPS---WKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQ-----------TLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKE----IIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDV 3404
+G KS R+ + ++ PTI G + G P V LN + C P + WF+D P++S+ + + +TL+I+ + DN +Y C A + + + EV V PP+ +G LS EV+ L L+C A + P A +RW + GQ + ++ + A+S +T+ K + S +D LD+ +N+ P F+ + ++ G+S +L+ E A PP TW+++ L+ + I L I AK D YT V VN+ GE + +++ P+I G + E ++ ++ ++ECS NPA P+ Y + + + E + K+ Q + SL I+ + D G Y CV N G K L P ++ P + + +SL C + P P ++W + + N+ + + RT L+I+ + +D G Y C A N G +K+ ++ P +
Sbjct: 2555 AGDKSKSFRLNVFVS-----PTIAGVDSDGSPEDVIVILNSPTSLVCEAYS-YPPATITWFKDGTPLESNRNIRILPGGRTLQIL--NAQEDNAGRYSCVATNEA--GEKIKHYEVK--VYIPPIIKKGDLLG-PGLSPKEVKIRVNSSLTLECEA-YAIPSASLRWYKD--GQPLKS----------DDHVTIAASGHTLQIKEAQISDTGRYTCVASNLAGEDELDF---------------------DVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPP-PTLTWYKDGRPLTSSDRVLILPGGRVLQIPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVLLPPVIKGANSDLPEEVTVLVNKSTQMECSSSGNPA-----------------------------------------------PRNYWQKDGQILLEDEHHKF--------QSDGRSLQIL-NAQITDTGRYVCVAEN-TAGSAKKYFNLNVHVPPSVIG-PNHEHLSVVVNHFISLNCEVSGFPPPDLSWL--KNEEPIKPNTNV-LTVPGGRT------LQIIRAKISDGGDYTCIAINQAGESKKKVSLTVHVPPSI 3057 HSP 4 Score: 58.151 bits (139), Expect = 1.636e-7 Identity = 46/151 (30.46%), Postives = 69/151 (45.70%), Query Frame = 3
Query: 2967 MTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKF----SKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRIN-ITRFRTGFYVAQLKIVNLQRTDHGSYVCKAEN--GKGSDSRKIEVSGTSTP 3398
+ LL I + D G Y CV N + T T + S P F S A +G V+L CY++ +P PK+ W R + + + ++ I++ RTG L I NL +D G+Y+C+AEN GK + V+G P
Sbjct: 753 LVGLLKIQETQDLDAGDYTCVAIN-------EAGRATGRLTLDVGSPPVFIQEPSDVAVEIGSNVTLPCYVQGYPEPKIKWR--RLDNMPVFSRPFSVSFISQLRTG----ALFISNLWASDKGTYICEAENQFGKIQSQTTVTVTGLVAP 890 HSP 5 Score: 56.6102 bits (135), Expect = 4.750e-7 Identity = 42/130 (32.31%), Postives = 61/130 (46.92%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNL----GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKG 3356
LI+ +V D G Y CV NN D T+ T + +LP F++ G+L GE++ L C P PK+TW F N + A+ F + ++L I + + D G+YVC AEN G
Sbjct: 4223 LILENVVLEDSGTYTCVANNAAGED-------TRIVTLAVHTLPTFTELPGDLSLNKGEQLRLSCKAVGIPLPKLTWTFN--NNIIPAH---------FDSINGHSELVIEKVSKEDSGTYVCTAENSVG 4334 HSP 6 Score: 55.8398 bits (133), Expect = 7.106e-7 Identity = 38/130 (29.23%), Postives = 58/130 (44.62%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSR 3368
L + V D G YRCVV++ G ++ LT + P++ +PK G G E+S+ C +P PK+ W + N + + R+R L I N D G+Y C A N G+D +
Sbjct: 576 LELRSVKIGDAGEYRCVVSSE-GGSAAASVFLTVQEKPKVTVMPKNQSFTG--GSEISIMCSATGYPKPKIVW---------TMNEMFIMGSHRYRM-TSEGTLFIKNAVPKDAGTYACLASNAAGTDKQ 692
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Hmcn1 (hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047]) HSP 1 Score: 88.9669 bits (219), Expect = 6.429e-17 Identity = 160/684 (23.39%), Postives = 264/684 (38.60%), Query Frame = 3
Query: 1734 VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMT--IGLKSLSGAEVENGKLLKLQCTAGESN--PPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN---VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKF--ERKEIIRQNAELECSVQSNPANPHMSYKWTK----LVPG-----KGYNDISSSDLVPAFT--IG---CNQSLPFSKE----------------YKNEKPKPYS----------KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDR---NDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGN------LGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKI--------EVSGTSTP-------DVPLEIQVVNKSQSQIEISWIQGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDT 3563
V +NH + C + G P P+L W ++ +PI+ + V +TL+ II I+D Y C I++ + + +V+L V PP G +SLS V G + L+C ESN PP V+ W N G+M+ + + N E+L+ G +L E+ D YV C N ++ K +NV P EG TL + PP TW +N + + VH +S +KL I + + NYT V N G++Q L I P + G + E ++ +N EL C+ P PH+ +W + +V G + D S+ ++ A T +G C + P +E K E K + KA P+ + K + S + S + + R +D+ +Y CV +N D +K ++ Q+ P A G G S+ C+ P+P M+W R ++ ++ ++ L+++ + D G Y C A N G S+ ++G+ P + PLE+ + +ISW++ DG +QT QV+ E ++ R +DT
Sbjct: 2975 VVVNHFISLNCE-VSGFPPPDLSWLKNEEPIKPNTNVLTVPGGRTLQ-IIRAKISDGG-DYTC----IAINQAGESKKKVSLTVHVPPSIKDHGSQSLSIVNVREGTSVSLEC---ESNAVPPPVITWSKN--GRMIPD--------STNVEILTG----------GQTLHIRRAEV----SDTGQYV-CRAINVAGRDD-----KNFHLNVYVPPTIEGPETEVIVETISNPVTLTCDATGIPP-PTITWLKNHKPIENSDPLEVHILS-GGSKLQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPPSVAGAEVPSEVSVLLGENVELVCNADGIP-TPHL--QWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGEEDRIFNLNVYVPPKIRGNKEEAEKLMALVDTSINIECKATGTPPPQINWLKNGLPLPISSHIRLLSAGQVVRIVRAQVSDIAVYTCVASNRAGVD-------SKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCFTDGTPAPSMSWL--RDGQPLAPDAHLTVSTQGM-------VLQLIKAETEDTGKYTCVATNEAGEVSKHFVLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMK--DGRPFLQTEQVQTLEGG--AILRVSSAQVEDT 3593 HSP 2 Score: 86.6557 bits (213), Expect = 3.225e-16 Identity = 139/621 (22.38%), Postives = 215/621 (34.62%), Query Frame = 3
Query: 1701 PTIQGYNVGKPVK--LNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDE--VTLIVLFPPMTIGLKSLSGAEVENGKLLKL-------QCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN-FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDK--FERKEIIRQNAELECSVQSNPA---------NPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMV-------------------KSQFEMT---------SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPL 3410
P++ G V V L N E+ C+ G P P LQW RD KPI + + + I ++ + KY C A ++P ++ L V PP + G + E KL+ L +C A PP + W N P+ + + LLSA G +++ D V+ V K + V P + + I +G S ++ P +W R+ L+ ++ST L + A+ D YT V N+ GE L + P I G + E I+ EL C PA P + + + + G +SS+ + C S P + K + + G E +F V + Q + T L I + D D Y CV +N I G + LT P I P+ L + ++L C P+P++TW R + V A S R +I GF L I + TD G Y+C A N G+D R+I++ P + +
Sbjct: 3244 PSVAGAEVPSEVSVLLGENVELVCNA-DGIPTPHLQWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVA------TNPAGEEDRIFNLNVYVPP------KIRGNKEEAEKLMALVDTSINIECKA-TGTPPPQINWLKNG----------LPLPISSHIRLLSA-----------GQVVRIV---------RAQVSDIAVYTCVASNRAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCF-TDGTPAPSMSWLRDGQPLAPDAHLTVSTQGMVLQLIKAETEDTGKYTCVATNEAGEVSKHFVLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMKDGRPFLQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTLECKSDAVPPPVIMWLKNREQLQATPRVRILSGGRYLQINNADLGDTANYTCVASN-IAGKTTREFNLTVNVPPSIGGGPQ--SLVTLLNKSIALECRAEGVPAPRITW---RKDGVVLAESHARYSI--LENGF----LHIESAHVTDTGRYLCMATNVAGTDRRRIDLQVHVPPSIAM 3807 HSP 3 Score: 65.0846 bits (157), Expect = 1.154e-9 Identity = 131/618 (21.20%), Postives = 228/618 (36.89%), Query Frame = 3
Query: 1641 SGIKSVQSRVKLHITYSPEKPTIQGYNV-GKP----VKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMT-----IGLKSLSGAEVE--NGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPS---WKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQ-----------TLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKE----IIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDV 3404
+G KS R+ + ++ PTI G + G P V LN + C P + WF+D P++S+ + + +TL+I+ + DN +Y C A + + + EV V PP+ +G LS EV+ L L+C A + P A +RW + GQ + ++ + A+S +T+ K + S +D LD+ +N+ P F+ + ++ G+S +L+ E A PP TW+++ L+ + I L I AK D YT V VN+ GE + +++ P+I G + E ++ ++ ++ECS NPA P+ Y + + + E + K+ Q + SL I+ + D G Y CV N G K L P ++ P + + +SL C + P P ++W + + N+ + + RT L+I+ + +D G Y C A N G +K+ ++ P +
Sbjct: 2555 AGDKSKSFRLNVFVS-----PTIAGVDSDGSPEDVIVILNSPTSLVCEAYS-YPPATITWFKDGTPLESNRNIRILPGGRTLQIL--NAQEDNAGRYSCVATNEA--GEKIKHYEVK--VYIPPIIKKGDLLG-PGLSPKEVKIRVNSSLTLECEA-YAIPSASLRWYKD--GQPLKS----------DDHVTIAASGHTLQIKEAQISDTGRYTCVASNLAGEDELDF---------------------DVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPP-PTLTWYKDGRPLTSSDRVLILPGGRVLQIPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVLLPPVIKGANSDLPEEVTVLVNKSTQMECSSSGNPA-----------------------------------------------PRNYWQKDGQILLEDEHHKF--------QSDGRSLQIL-NAQITDTGRYVCVAEN-TAGSAKKYFNLNVHVPPSVIG-PNHEHLSVVVNHFISLNCEVSGFPPPDLSWL--KNEEPIKPNTNV-LTVPGGRT------LQIIRAKISDGGDYTCIAINQAGESKKKVSLTVHVPPSI 3057 HSP 4 Score: 58.151 bits (139), Expect = 1.580e-7 Identity = 46/151 (30.46%), Postives = 69/151 (45.70%), Query Frame = 3
Query: 2967 MTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKF----SKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRIN-ITRFRTGFYVAQLKIVNLQRTDHGSYVCKAEN--GKGSDSRKIEVSGTSTP 3398
+ LL I + D G Y CV N + T T + S P F S A +G V+L CY++ +P PK+ W R + + + ++ I++ RTG L I NL +D G+Y+C+AEN GK + V+G P
Sbjct: 753 LVGLLKIQETQDLDAGDYTCVAIN-------EAGRATGRLTLDVGSPPVFIQEPSDVAVEIGSNVTLPCYVQGYPEPKIKWR--RLDNMPVFSRPFSVSFISQLRTG----ALFISNLWASDKGTYICEAENQFGKIQSQTTVTVTGLVAP 890 HSP 5 Score: 56.6102 bits (135), Expect = 4.627e-7 Identity = 42/130 (32.31%), Postives = 61/130 (46.92%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNL----GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKG 3356
LI+ +V D G Y CV NN D T+ T + +LP F++ G+L GE++ L C P PK+TW F N + A+ F + ++L I + + D G+YVC AEN G
Sbjct: 4223 LILENVVLEDSGTYTCVANNAAGED-------TRIVTLAVHTLPTFTELPGDLSLNKGEQLRLSCKAVGIPLPKLTWTFN--NNIIPAH---------FDSINGHSELVIEKVSKEDSGTYVCTAENSVG 4334 HSP 6 Score: 55.8398 bits (133), Expect = 7.281e-7 Identity = 38/130 (29.23%), Postives = 58/130 (44.62%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSR 3368
L + V D G YRCVV++ G ++ LT + P++ +PK G G E+S+ C +P PK+ W + N + + R+R L I N D G+Y C A N G+D +
Sbjct: 576 LELRSVKIGDAGEYRCVVSSE-GGSAAASVFLTVQEKPKVTVMPKNQSFTG--GSEISIMCSATGYPKPKIVW---------TMNEMFIMGSHRYRM-TSEGTLFIKNAVPKDAGTYACLASNAAGTDKQ 692
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Cd22 (CD22 antigen [Source:MGI Symbol;Acc:MGI:88322]) HSP 1 Score: 73.9442 bits (180), Expect = 1.467e-12 Identity = 82/298 (27.52%), Postives = 130/298 (43.62%), Query Frame = 3
Query: 1623 LDCQAWSGIKSVQSR-VKLHITYSPE-----KPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPE---LQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGA-EVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNS 2486
+ CQ K + R V+L + Y+P+ PT V+ N++ MTC NP + WF+D +P++ + Q+ Q+ K+I+ D + KY CQA S P S+EV L V + P + + +S A E+ G+ + LQC ESN PA VR+ + G ++ E + E+ S + + +N G +L Q++ QVL ++ V+ SP ++ EG+ TL+ E ANPPI YTWF +S
Sbjct: 233 VKCQVQHSSKVLSERTVRLDVKYTPKLEIKVNPT--------EVEKNNSVTMTCRVNSSNPKLRTVAVSWFKDGRPLEDQELEQE---QQMSKLILHSVTKDMRGKYRCQA---SNDIGPGESEEVELTVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESN-PAEVRFFWKKNGSLVQEGRYLSFGSVSPED--SGNYNCMVNNSIGETLSQAW-------------------------NLQVLYAPRRLRVSISP--------GDHVMEGKKATLSCESDANPPISQYTWFDSS 480
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q9QZS7|NPHN_MOUSE (Nephrin OS=Mus musculus OX=10090 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 226.483 bits (576), Expect = 1.636e-58 Identity = 274/1166 (23.50%), Postives = 455/1166 (39.02%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD-----NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVIS-YQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQ-----QVGN--------QKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRI----QINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTG-FYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE-KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTN--TMSADNEKENNQPTYPIIQ 3740
V +GS L C VR VQW +LLG + IPG P Y + G KGE L I + D YECQV G+ + V+L P++ P + Q+ TCVS + +P + ++D NE S E+L ++ + + +G ++ C SN A P + S + + PPG P ++D +N+ + C + G P +L W V + + Q ++ + +V T+ ++HGA+L C + N V + Q ++ L + P+ V I +G +EN + T+ C T ++W + D ++ + S S + + + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W +DS+P+ +Q Q+G+ + L +II P DN K+ C+A +S + L+V FPP + + + S A + G L L C + SNPP V L++ G+ + ++ +P + F GS + S +DH V C + + LR+ + ++NV + P+F G + +E+GQ+ L + ANP + + W +LS H I S L + N D Y L N G ++ + L++ Y P I K + + + ++ C+V +NP P M + W +L G+ +++ D M K T L I + G Y+C+V+NG+ + L F PQ+ +K AAG+ +L C R P+ TW N R ++ G + + L I N+ D+ + C A N GSD I++ S PD PL ++VV+ S + + W GFDGG Q FQ+R++ +SP LY DV +F +TGLK T Y I + + N+ + K I + T D E+ P Q
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV---GRSELGPELVSPSVILSILVSPKVLQ--LTPEAGSTVTWVAGQEYVV--TCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSE--EKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILF----PPGPP-VIDWPGLNEGHVRAGENLELPCIAR-GGNPPATLQWLKNGKPVSIAWGTE-------------HAQAVAHSVLVMTVR-----PEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI-----------LGSTSQSEN-------KNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETV-MDGLHGGHISM---SNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPP--DNLAKFSCKAGQLSASTQ--------LVVQFPPTNLTILANSSA-LRPGDALNLTCVSISSNPP--VNLSLDKEGERLDDVAAKPQ-----------------SAPFKGSAASRSVFLRVSSRDHGHRVTCRAHS-------EALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRI-LSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEM-FSWERL--GEDEEELNLDD-----------------------------------------------MEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTW---TKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYM--DVLPAQAT---TFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQ 1067
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 224.942 bits (572), Expect = 5.050e-58 Identity = 262/1172 (22.35%), Postives = 455/1172 (38.82%), Query Frame = 3
Query: 351 VAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD---NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKED--ESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQ-----------VGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKT----IMTNTM-SADNEKENNQPTYPIIQPNKLQDNTILF 3773
V +G++ LRC V VQW +LLG D IPG P YR+ G +GE L I + D YECQV + P L ++ ++ P P++ P ++ Q+ CVS + +P L+ I D N N ++L + + + ++ C S+ A E P + S + V PPG P +++ ++ + C + G P +L W V + + Q ++++ +V T+ ++HGAQL C A N V Q + L + P+ ++I +G EN + T++C + ++W + + P+E+ + S + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L W++DS+ + S + Q+ G+ + ++++ +DN+ K+ C+A +S + L V FPP + + + + A + G L L C + SNPP + W ++ G+ + + P + F GS + S +DH V C + E + + ++NV + P+F G + +E+G++ L + ANP + + W +LS H I +S A L + N D Y L N G ++ + L++ Y P I + + + + ++ C+V +NP P M + W +L G+ D S D M K T L I+ G Y+C+V+NG+ + + L F PQ+ +K AAG+ +L C R P+ TW + + T + G + + L I N+ D+ + C A N GSD I++ S PD P ++VV+ + + + W GFDGG Q F +R+ E T + Y DV +F +TGL+ T Y + + + N+ + K I T + E E+ PT P P+ L +LF
Sbjct: 43 VVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVG-RSEMGPELVSPRVILSILVP--PKLLL--LTPEAGTMVTWVAGQEY--VVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEA---PIKASFTVNVLF----PPGPP-VIEWPGLDEGHVRAGQSLELPCVAR-GGNPLATLQWLKNGQPVSTAWGTE-------------HTQAVARSVLVMTVR-----PEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII-----------LGSASQTEN-------KNVTLSCVSKSSRPRVLLRWW------LGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQ--------LAVQFPPTNVTILANASA-LRPGDALNLTCVSVSSNPPVNLSW--DKEGERLEGVAAPPRRA-----------------PFKGSAAARSVLLQVSSRDHGQRVTCRAHS---AELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEA-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGA-LHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGM-FNWERL--GEDEEDQSLDD-----------------------------------------------MEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWT--KNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY-EALGTPGFHYVDVVPPQAT---TFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLF 1067
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q9R044|NPHN_RAT (Nephrin OS=Rattus norvegicus OX=10116 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 224.557 bits (571), Expect = 8.071e-58 Identity = 273/1158 (23.58%), Postives = 452/1158 (39.03%), Query Frame = 3
Query: 357 QGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQD---NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGI---KSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQ-----QVGN--------QKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRI----QINVTFSPQFEG-IINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKN--VHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSK--AAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTG-FYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTN--TMSADNEKENNQPTYP 3731
+G+ L C VR VQW +LLG + +PG P Y + G KGE L I + D YECQV G+ + V+L P++ P + Q+ TCVS + +P + + I D N N E+L + + + +G ++ C SN A + P + S + + PPG P ++D +N+ + C + G P +L W V + + Q ++ + +V T+ ++HGA+L C + N V Q ++ L + P+ + I GS+S N+NV T+ C T ++W + D ++ + S S + + ++ + L C+A+S ++ + + L++ Y +K I+G G+ ++ + C IGGNP P L WF+DS+P+ Q Q+G+ + L +II P DN+ K+ C+A +S + L+V FPP + + + S A + G L L C + SNPP + W ++ G+ + ++ +P + F GS + S +DH V C + + LR+ + + NV + P+F G + +E+GQ L + ANP + + W +LS H I S L + N D Y L N G ++ + L++ Y P I + + + + ++ C+V +NP P M + W +L G+ D++ D M K T L I + G Y+C+V+NG+ + L F PQ+ +K AAG+ +L C R P+ TW N R R+ G + + L I N+ D+ + C A N GSD I++ S PD PL ++VV+ S + + W GFDGG Q FQ+R+ E T + + DV T+ T +F +TGLK T Y I + + N+ + K I + T D E+ P
Sbjct: 59 EGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQV---GRSELGPELVSPKVILSILVSPKVLL--LTPEAGSTVTWVAGQEYV--VTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDT---PIKASFTMNILF----PPGPP-VIDWPGLNEGHVRAGENLELPCTAR-GGNPPATLQWLKNGKPVSTAWGTE-------------HAQAVAHSVLVMTVR-----PEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITIL---GSVSQSE------NKNV---------TLCCLTKSSRPRVLLRWWLGGRQLLPTDETV-MDGLHGGHISM---SNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPP--DNRAKFSCKAGQLSASTQ--------LVVQFPPTNLTILANSSA-LRPGDALNLTCVSISSNPPVNLSW--DKEGERLEDVAAKPQ-----------------SAPFKGSAASRSVFLRVSSRDHGQRVTCRAHS-------EALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQV-LLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRI-LSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEM-FSWERL--GEEEEDLNLDD-----------------------------------------------MEKVSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTW---TKNGVPLDLQDPRYTEHRYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRY-EALETPGFLHVDVLP--TQAT-TFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSVTTPGPDQAPEDTDHQLP 1064
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q9U3P2|SYG2_CAEEL (Synaptogenesis protein syg-2 OS=Caenorhabditis elegans OX=6239 GN=syg-2 PE=1 SV=1) HSP 1 Score: 91.2781 bits (225), Expect = 7.123e-17 Identity = 188/891 (21.10%), Postives = 330/891 (37.04%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKV---QWRA-VPVLLGYDRN--IPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQ-IFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYS---RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQI-VDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISY-QSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFS----PNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPEL-----------------QW----------------------FRDSKPIQ-----SSFIAQQVGNQK----------TLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE--VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPV---DIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTK 2762
Q F E P + +A G + T RC + QW++ LLGY + +PG E L+I++V + D G YECQ+ HP L PQ +++ +QPN I ++K++ TCV N +P+ + W+++ ++ + A LN + + L +++ + G VI C + T++ ++T + + PP P + + NP + DN+ + C S G P + W + N R ++++T D +++K + + + ++ A+ C A N + A+KL + + P V + G+ N+ YG + I C + P + I W+ + P P +F+ + S S +++ + Q ++C A + S + + I P+ P I G K + +TC GGNP EL +W FR SK + + FI V TL + + ++N ++K C+A + +L + S ++ V +PP + ++ + + GK +L C SNP A + W+ +R T N+ L S N + G +++ P+ + V C +P++ +H V +NV + P+ + EG S NL NP I + W +N H+I A+ + + K + D YTL N G + ITI L + Y I LEC P+ +M + W K
Sbjct: 19 QFFLESPSNLSTIA-GESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQML-----HPEEGPIRAKSFLNIIVPPQLVYFSNYQPNS----IIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIR-------ITTNTTLDVLFPPSDPTVEILRNP---SALRSGDNVTIAC-SVTGGNPPPDVFWYHE--------NKRLQSHSTLD----------TRSKEIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVEL-----------FGES----NIRYGSSAN---IQCKSLPSNPASQITWIINGRSVPTPT-QREFV-----VENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKAPIITGLEDRKFFE-GDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVKKKLVMSLLCLCEVVIMNFFRFSKNVFYFFRFTLFIECGVNGAHNEVAGFSSYSTLALRVDRTMNTQRLK--CEATNAALDEPLIESQYLS--VYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRA-------------TDDNKVHLGDVSLNETT-RDNGFNVENVLSFTPTEEYDGTVVHCIANHPEW--KHSV-NTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD----HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-WYK 819 HSP 2 Score: 83.5741 bits (205), Expect = 1.661e-14 Identity = 68/275 (24.73%), Postives = 121/275 (44.00%), Query Frame = 3
Query: 2889 KAEWKGTPEKSNFKYFSK---CMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANS-KYRINITRFRTGFYVAQLKIVNLQRTDHGSYV-CKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD 3698
+ E G P K+N ++ K + ++ + G Y C +NGI L P++L + +++K AG LG C + + P+ + W + + NS KY I T+ Y + L I N+ D+ V C A N G+D I + + PD P + + N + + I ++W FDGGS Q F+V+++ ++ ++ V + T + LS GL + Y I S N++ S ++ ++ T++ D
Sbjct: 802 ECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVW--EKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHL---VNTTHTNLRLS----GLATANTYFFQIRSINARGFASSWTSPVIFATLNED 1067
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|B1Q236|SYG1_CAEEL (Synaptogenesis protein syg-1 OS=Caenorhabditis elegans OX=6239 GN=syg-1 PE=1 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 1.109e-11 Identity = 87/334 (26.05%), Postives = 140/334 (41.92%), Query Frame = 3
Query: 327 EIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRN--IPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQ-------IVDMNPHKTTPYVVD-DNIIMTCESSPSGKPAGSLLWRWKYPKVRE----LYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV 1286
E PK A G A L C V +Q G VQW LG DR+ +PG YR+VGS GE+ LEISNVT+ D + CQ+ ++ K KL VLVRP P+I + +I D T+ +C+S G+P W + D ++ L SRS+ +K + N T+V + + ++ ++ P+ ++ ++ H T P ++ D++ ++ +P +L K P +RE L ++ +Y+ + TLT L ++H +FC ATN +
Sbjct: 27 EAPKDTL-AAVGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKIVKSHHS-------LKAIAGDPITQ-SCLSRKGKPPPTIGWA----IASDEHGKHIVSWLGESRSKFGGIHAKPEISQETVIAHVNETTQVEEGGNNSREDSSIYSIMSNLSFIPRPEDDHKYLICISQHMTFPNKIEVDSVKLSLRYAPQ----INLTVASKLP-LRENGSALLACNVNAKPLDNVKISWYKGNQKLRETGDTLTFETLKMEDHNRDIFCEATNEI 342
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A0H3YFQ6 (NPHS1-6 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-6 PE=2 SV=1) HSP 1 Score: 3256.46 bits (8442), Expect = 0.000e+0 Identity = 1670/1670 (100.00%), Postives = 1670/1670 (100.00%), Query Frame = 3
Query: 126 MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASXXXXXXXXXXXXXXXXXXXXXXXXXXTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIAXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXXXXXXXXXXXXXPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 5135
MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII
Sbjct: 1 MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 1670
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A4E0RDV1 (Nephrin OS=Fasciola hepatica OX=6192 GN=D915_004791 PE=4 SV=1) HSP 1 Score: 781.171 bits (2016), Expect = 0.000e+0 Identity = 466/1295 (35.98%), Postives = 672/1295 (51.89%), Query Frame = 3
Query: 204 HP---DQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPV-GQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNN---------------NLIQD---NENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW---------------------------KYPKVRELYNNRFENY-----------------TTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVF-SPNPAI---NNDFISPVEKIWKEDESFR-RNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCG-------------QMMSELGRRPVQTT-CNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGI-INYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL-----SRKNVHSISTSKAK---LIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFER-KEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGY-NDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSV---SANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENN-QPTYPIIQPNKLQDNTILFIVAA 3785
HP D FD S LTD ++ QS ECT +E NRIQCF ++P+ Y V+ G+ ++C+V NQ+GKVQWRA +LLGYDR+IPG YRV+G V +GEH L+I +V ++DAG YECQV PV HPLLRRKT+L+VL++P P I Y P Q+II+ +R CVS G P F W LNN +L+Q+ + + +SRL+L S KDGD + C V+N AT++H DP +R+LST V I +HTPPG P+I+ +P Y+ D + C +SP G P G L WRW Y V + + Y ++D+ P +Y K+++V+TL IP +T + H ++L C +PV + T++ +Y+ H P V IS+ G++ G E V+YG AGE KTI C T PYY +A IKWV P+P + + + I D R + S + + I + D+ ++DC+ W G + +RV+L I Y P KPT+ GY P+K+ H E+ C T GGNP PEL WF+D++ I S+ +G Q +LK+ + P DN Y C +++ + + S+ V L VLFPP I + S V +G L + C A SNP A + W RCG Q S GR P + T C + ++ G GG S + P+W H+DY++C NP+YG V R+Q+N+TF+PQF G+ + EGQS +L+L P ANPP+ WF N L K V + S L + K + NYT++ N ES+ +LN+TY +IG + + + A EC+ ++NPA S+ W + VP K + D + L I CN+S P +++ K F +C +SQ M+S L +Y V +DVG Y C VNNG+ V K + F P ++ LP+++KAAG+ G +VSL C+IR+ P PK+ W G V ++SKY+I + R G Y A L + NLQ+TD G Y C+A N +G + I++SGTSTPDVPL ++++N + + ++++W QGFDGG QTFQVRW++ S+YRY DVA++D + ++IT L+ T Y IS+NS NSKHG S F++ I T D++ +P P+ DN +L IVAA
Sbjct: 77 HPLLSDPLFDSSILTDD----AGRVIQSSECTPQERQNRIQCFDKLPEDKYAVSLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSIPGYQRYRVIGDVGRGEHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELIVLIKPSVPSILYPDVPPLDRQLIISKPDPILRITVLCVSVGGSPPPSFSWSLNNQEIPQIRTTPSPRRMSLVQNPWMKLEPVIETQPETGQSRLTLLKSGLKDGDQLMCSVTNAATQLHHDPHQRNLSTHVTIRVHTPPGPPRII--SPETDHVYLEGDELKAICIASPPGNPLGGLFWRWLLHPVQVNDPSIGHVSGLGGRGGARLSDYSSVEAYLHAQDVGYLPGEKSGTTSPHQTLTPISEDVQPLHYTLNKDKDQLVNTLVIPRITRRYHASKLVCETGHPVGTAHQTSINIYVKHPPANVTISIDGGTVRQEIAA--GRREYVVYGRAGEMKTIICRTGPYYRDAQIKWVAQGPDPGLLKAPRELFGQTKTIQTSDGYSRVQESRVNVTITEADDRGYIDCRVWGEGDTRMDARVRLDIIYPPTKPTVSGYKSLDPIKMAHTLELVCITYGGNPPPELAWFKDNQQILSTSDVVVLGRQSSLKLKVIPQKEDNGAHYHCSSRNAATGPEGIRSESVILNVLFPPERIVISFHSPPVVSSGHQLVINCQADTSNPVATISWWHFRCGPAHAFTQSLVSSGQKKSNAGRTPTENTKCKSVAMQGTNEKPTPGAHGGLQANSRLWLRPTWHYHMDYIECRTENPEYGPP--VWHDRLQLNITFAPQFLGLQAGTEKVVREGQSMSLDLGPYANPPVTAVNWFINGVPLLFEPSDTKEVEGVFASGRNGELLALHRIKRKHMANYTVMATNPEAESKAVFFLNVTYPAELIGSTVDNITDSVNGVASFECTAEANPAVAIRSFTWYRFVPPKSWPEDSHEAALQLGSPIPCNES--------------------TSAPGRTD-KLFVRCQQESQLRMSSRLSLYRVTEDDVGTYVCEVNNGLGEPVQKRVNFLHPFPPTVIRLPRYTKAAGDQGSQVSLTCFIRTEPGPKVAWLKGGQLIQVPEGQSHSKYQIGLKHIRPGLYKATLVLNNLQKTDFGKYQCQAVNLQGEATLDIQMSGTSTPDVPLNLRLLNATMNTLKVAWTQGFDGGLSQTFQVRWRQAETGSMYRYADVAANDNHNAVEYLITALQPGTEYTISVNSMNSKHGASAFTEPIHVRTEFYDDQTGKALRP--PLSSSGHSDDNALLIIVAA 1338
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A3Q0KLM5 (Putative nephrin OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 771.541 bits (1991), Expect = 0.000e+0 Identity = 462/1335 (34.61%), Postives = 703/1335 (52.66%), Query Frame = 3
Query: 153 LKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMS-SGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVG-QEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLN-----------------------NNLIQ--DNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW-------------KYPKVRELYNNRFENYTT------------------------------------KDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWK-------EDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQT---------------TCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGI-INYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL--------SRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFER-KEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYND-ISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFF------------GRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD--NEKENNQPTYPIIQPNKLQDNTILFIVAA 3785
L F++ +LIG F+ ++ + + S +S + A++ QS CT EE RIQCF+ +P Y + G ++C+V NQ+GKVQWRA +LLGYDR+IPG +R++G V +GEH L+I++V DAG YECQV PV HPLLRRKT L VL++P++PQI Y+ Q ++II+ +R C + G+P F+W +N NNL++ + + E N +S LS+ + +DGD I C VSN AT+++ DP +R+LS + + +HTPPG P+IV +P Y+ + + C SSP GKP G L WRW K + R +YT+ +D+ P +Y T K+++V+TL I +T K H A+L C +PV S T++ +++ H P V ISV GS+ G G +E V Y AGE KT C T PYY +ATIKW+F A + SP + + K + ESF + S I+I + + D+S++DC WS G + SRV+L I + P+ P I GY G+PV + H E+TC+T GGNP PELQWF+D PI ++ +G Q T+K+ + P DN +Y C A++ + + ++S+ +TL VLFPP + L + V++G+ L++ C A SNP AV+ W+ +CG + R +Q C L S + G GG L +S+ + P+W+ H+D+++C+ NP YG + R+Q+N+TF+PQF G+ ++ + I EG++ L+ ANPP+ +WFRN L + + V + + L + ++ NYT++V N + ES+ T +LN+TY I+G E + + A L+C +NPA P ++ W + P KG+ + + L + I CNQS + +KPK ++C + +F+++S IY + +DVG Y C V+NGI V KT L F P+I+ LP+++KAAG +V L CYIR+ P+P++ W + + S + KY + R G Y AQL + ++++ D GSY C+A N +G +I +SGTSTPDVP+ +++N + S + ++W QGFDGG QTFQVRW+E SLY+Y DVAS+D + + +IITGLK + Y++S+NS NSKHG S ++ I T+S D N +E P P+ DN +L IV+A
Sbjct: 33 LHIFIITLLIGWFIDFALAEKHECNQNTCYSVLSDTAARVIQSNRCTTEERQKRIQCFEVLPSEQYAIPLGHTVNMQCVVLNQHGKVQWRAKKILLGYDRSIPGYSRFRIIGDVGRGEHTLQITDVQRDDAGEYECQVTPVPVNNHPLLRRKTYLEVLIKPNEPQILYQNVQLPERKLIISQPDPILRISLVCSAVGGRPAPTFKWLINRHEIPNLEVKTKTPMSPNDVAKMNNLLKVWGKLHPIINNEENEEQSNLSILKAGLEDGDEIVCSVSNSATQINHDPMKRNLSITITVDVHTPPGPPKIV--SPESNHVYLDGEQLRAVCVSSPPGKPLGGLFWRWLIRPSQVDDNDIQKISGIGGRGGARLSDYTSVEAYLRSLDSSNGGGSPSQKILTPELSQLQQGDFISEDVQPLHYTLTKEKDQLVNTLIIQRVTRKYHAAKLICETGHPVGSAHQTSITIFVKHAPANVTISVETGSIDDDHTG--GRHEKVAYARAGEMKTFICKTAPYYGQATIKWLFQA--AGTSITTSPKQLVDKSVTYKTPDGESFYQESRIQITVRPEDDRSYIDCLVWSVGDARIDSRVRLDIIHPPDIPKITGYKSGQPVNMAHLLELTCTTTGGNPLPELQWFKDKNPITNNVEPIIIGKQTTIKLKLVPQKEDNGAQYHCSARNTATNNEWIDSEAITLNVLFPPQRVSLNFGGKSVVQSGEQLEINCQADSSNPVAVITWRHYQCGPAHAFYRRHLLQQQKKLPKSSSSTNEGEKCKLLELKGSDETPVPGASGGLLARSHLWLRPTWRYHMDFIECQAENPVYGPP--IWHDRLQLNITFAPQFYGLQVDDHRVIREGETTHLDFGLYANPPVTSVSWFRNDQLLPFEPKETDTWQGVFAAGSVGELLSLHKINRENMGNYTVIVSNSQHESRNTFFLNVTYPASIVGKLEENITQTNKDYAALDCKADANPAIPDRTFTWYRYFPPKGWTEEVDEGALQLSEPIYCNQS-------QIDKPK-----------------MLAQCFQQDKFQISSTFYIYQLTEDDVGKYVCEVSNGIGEPVKKTFNLLYHFPPKIIQLPRYTKAAGEQSSKVWLTCYIRTEPTPQIVWLKDNKLIPMDTLIQSKNSDSKNGIKKYENYLIHIRPGLYKAQLAVNDIRKYDFGSYSCQAANLQGEAQLEIHLSGTSTPDVPINFRLLNATSSTLHVAWTQGFDGGLAQTFQVRWREVGSISLYKYADVASNDNRNGVEYIITGLKPGSEYIVSVNSMNSKHGASAYTDPIHLRTLSLDLYNGRE---PMPPLSTSGYSDDNALLIIVSA 1332
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A5K4EMM3 (Putative nephrin OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 771.541 bits (1991), Expect = 0.000e+0 Identity = 465/1337 (34.78%), Postives = 706/1337 (52.80%), Query Frame = 3
Query: 153 LKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMS-SGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVG-QEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLN-----------------------NNLIQ--DNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW-------------KYPKVRELYNNRFENYTT------------------------------------KDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWK-------EDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQT---------------TCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGI-INYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL--------SRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQN---AELECSVQSNPANPHMSYKWTKLVPGKGYND-ISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFF------------GRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD--NEKENNQPTYPIIQPNKLQDNTILFIVAA 3785
L F++ +LIG F+ ++ + + S +S + A++ QS CT EE RIQCF+ +P Y + G ++C+V NQ+GKVQWRA +LLGYDR+IPG +R++G V +GEH L+I++V DAG YECQV PV HPLLRRKT L VL++P++PQI Y+ Q ++II+ +R C + G+P F+W +N NNL++ + + E N +S LS+ + +DGD I C VSN AT+++ DP +R+LS + + +HTPPG P+IV +P Y+ + + C SSP GKP G L WRW K + R +YT+ +D+ P +Y T K+++V+TL I +T K H A+L C +PV S T++ +++ H P V ISV GS+ G G +E V Y AGE KT C T PYY +ATIKW+F A + SP + + K + ESF + S I+I + + D+S++DC WS G + SRV+L I + P+ P I GY G+PV + H E+TC+T GGNP PELQWF+D PI ++ +G Q T+K+ + P DN +Y C A++ + + ++S+ +TL VLFPP + L + V++G+ L++ C A SNP AV+ W+ +CG + R +Q C L S + G GG L +S+ + P+W+ H+D+++C+ NP YG + R+Q+N+TF+PQF G+ ++ + I EG++ L+ ANPP+ +WFRN L + + V + + L + ++ NYT++V N + ES+ T +LN+TY I+G K E + I + N A L+C +NPA P ++ W + P KG+ + + L + I CNQS + +KPK ++C + +F+++S IY + +DVG Y C V+NGI V KT L F P+I+ LP+++KAAG +V L CYIR+ P+P++ W + + S + KY + R G Y AQL + ++++ D GSY C+A N +G +I +SGTSTPDVP+ +++N + S + ++W QGFDGG QTFQVRW+E SLY+Y DVAS+D + + +IITGLK + Y++S+NS NSKHG S ++ I T+S D N +E P P+ DN +L IV+A
Sbjct: 33 LHIFIITLLIGWFIDFALAEKHECNQNTCYSVLSDTAARVIQSNRCTTEERQKRIQCFEVLPSEQYAIPLGHTVNMQCVVLNQHGKVQWRAKKILLGYDRSIPGYSRFRIIGDVGRGEHTLQITDVQRDDAGEYECQVTPVPVNNHPLLRRKTYLEVLIKPNEPQILYQNVQLPERKLIISQPDPILRISLVCSAVGGRPAPTFKWLINRHEIPNLEVKTKTPMSPNDVAKMNNLLKVWGKLHPIINNEENEEQSNLSILKAGLEDGDEIVCSVSNSATQINHDPMKRNLSITITVDVHTPPGPPKIV--SPESNHVYLDGEQLRAVCVSSPPGKPLGGLFWRWLIRPSQVDDNDIQKISGIGGRGGARLSDYTSVEAYLRSLDSSNGGGSPSQKILTPELSQLQQGDFISEDVQPLHYTLTKEKDQLVNTLIIQRVTRKYHAAKLICETGHPVGSAHQTSITIFVKHAPANVTISVETGSIDDDHTG--GRHEKVAYARAGEMKTFICKTAPYYGQATIKWLFQA--AGTSITTSPKQLVDKSVTYKTPDGESFYQESRIQITVRPEDDRSYIDCLVWSVGDARIDSRVRLDIIHPPDIPKITGYKSGQPVNMAHLLELTCTTTGGNPLPELQWFKDKNPITNNVEPIIIGKQTTIKLKLVPQKEDNGAQYHCSARNTATNNEWIDSEAITLNVLFPPQRVSLNFGGKSVVQSGEQLEINCQADSSNPVAVITWRHYQCGPAHAFYRRHLLQQQKKLPKSSSSTNEGEKCKLLELKGSDETPVPGASGGLLARSHLWLRPTWRYHMDFIECQAENPVYGPP--IWHDRLQLNITFAPQFYGLQVDDHRVIREGETTHLDFGLYANPPVTSVSWFRNDQLLPFEPKETDTWQGVFAAGSVGELLSLHKINRENMGNYTVIVSNSQHESRNTFFLNVTYPASIVG-KLE-ENITQTNKDYAALDCKADANPAIPDRTFTWYRYFPPKGWTEEVDEGALQLSEPIYCNQS-------QIDKPK-----------------MLAQCFQQDKFQISSTFYIYQLTEDDVGKYVCEVSNGIGEPVKKTFNLLYHFPPKIIQLPRYTKAAGEQSSKVWLTCYIRTEPTPQIVWLKDNKLIPMDTLIQSKNSDSKNGIKKYENYLIHIRPGLYKAQLAVNDIRKYDFGSYSCQAANLQGEAQLEIHLSGTSTPDVPINFRLLNATSSTLHVAWTQGFDGGLAQTFQVRWREVGSISLYKYADVASNDNRNGVEYIITGLKPGSEYIVSVNSMNSKHGASAYTDPIHLRTLSLDLYNGRE---PMPPLSTSGYSDDNALLIIVSA 1332
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A5K4EMI7 (Putative nephrin OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 769.615 bits (1986), Expect = 0.000e+0 Identity = 461/1335 (34.53%), Postives = 701/1335 (52.51%), Query Frame = 3
Query: 153 LKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMS-SGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVG-QEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLN-----------------------NNLIQ--DNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRW-------------KYPKVRELYNNRFENYTT------------------------------------KDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWK-------EDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQT---------------TCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGI-INYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKL--------SRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFER-KEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYND-ISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFF------------GRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSAD--NEKENNQPTYPIIQPNKLQDNTILFIVAA 3785
L F++ +LIG F+ ++ + + S +S + A++ QS CT EE RIQCF+ +P Y + G ++C+V NQ+GKVQWRA +LLGYDR+IPG +R++G V +GEH L+I++V DAG YECQV PV HPLLRRKT L VL++P++PQI Y+ Q ++II+ +R C + G+P F+W +N NNL++ + + E N +S LS+ + +DGD I C VSN AT+++ DP +R+LS + + +HTPPG P+IV +P Y+ + + C SSP GKP G L WRW K + R +YT+ +D+ P +Y T K+++V+TL I +T K H A+L C +PV S T++ +++ H P V ISV GS+ G G +E V Y AGE KT C T PYY +ATIKW+F A + SP + + K + ESF + S I+I + + D+S++DC WS G + SRV+L I + P+ P I GY G+PV + H E+TC+T GGNP PELQWF+D PI ++ +G Q T+K+ + P DN +Y C A++ + + ++S+ +TL VLFPP + L + V++G+ L++ C A SNP AV+ W+ +CG + R +Q C L S + G GG L +S+ + P+W+ H+D+++C+ NP YG + R+Q+N+TF+PQF G+ ++ + I EG++ L+ ANPP+ +WFRN L + + V + + L + ++ NYT++V N + ES+ T +LN+TY I+G E + + A L+C +NPA P ++ W + P KG+ + + L + I CNQ +KPK ++C + +F+++S IY + +DVG Y C V+NGI V KT L F P+I+ LP+++KAAG +V L CYIR+ P+P++ W + + S + KY + R G Y AQL + ++++ D GSY C+A N +G +I +SGTSTPDVP+ +++N + S + ++W QGFDGG QTFQVRW+E SLY+Y DVAS+D + + +IITGLK + Y++S+NS NSKHG S ++ I T+S D N +E P P+ DN +L IV+A
Sbjct: 33 LHIFIITLLIGWFIDFALAEKHECNQNTCYSVLSDTAARVIQSNRCTTEERQKRIQCFEVLPSEQYAIPLGHTVNMQCVVLNQHGKVQWRAKKILLGYDRSIPGYSRFRIIGDVGRGEHTLQITDVQRDDAGEYECQVTPVPVNNHPLLRRKTYLEVLIKPNEPQILYQNVQLPERKLIISQPDPILRISLVCSAVGGRPAPTFKWLINRHEIPNLEVKTKTPMSPNDVAKMNNLLKVWGKLHPIINNEENEEQSNLSILKAGLEDGDEIVCSVSNSATQINHDPMKRNLSITITVDVHTPPGPPKIV--SPESNHVYLDGEQLRAVCVSSPPGKPLGGLFWRWLIRPSQVDDNDIQKISGIGGRGGARLSDYTSVEAYLRSLDSSNGGGSPSQKILTPELSQLQQGDFISEDVQPLHYTLTKEKDQLVNTLIIQRVTRKYHAAKLICETGHPVGSAHQTSITIFVKHAPANVTISVETGSIDDDHTG--GRHEKVAYARAGEMKTFICKTAPYYGQATIKWLFQA--AGTSITTSPKQLVDKSVTYKTPDGESFYQESRIQITVRPEDDRSYIDCLVWSVGDARIDSRVRLDIIHPPDIPKITGYKSGQPVNMAHLLELTCTTTGGNPLPELQWFKDKNPITNNVEPIIIGKQTTIKLKLVPQKEDNGAQYHCSARNTATNNEWIDSEAITLNVLFPPQRVSLNFGGKSVVQSGEQLEINCQADSSNPVAVITWRHYQCGPAHAFYRRHLLQQQKKLPKSSSSTNEGEKCKLLELKGSDETPVPGASGGLLARSHLWLRPTWRYHMDFIECQAENPVYGPP--IWHDRLQLNITFAPQFYGLQVDDHRVIREGETTHLDFGLYANPPVTSVSWFRNDQLLPFEPKETDTWQGVFAAGSVGELLSLHKINRENMGNYTVIVSNSQHESRNTFFLNVTYPASIVGKLEENITQTNKDYAALDCKADANPAIPDRTFTWYRYFPPKGWTEEVDEGALQLSEPIYCNQI---------DKPK-----------------MLAQCFQQDKFQISSTFYIYQLTEDDVGKYVCEVSNGIGEPVKKTFNLLYHFPPKIIQLPRYTKAAGEQSSKVWLTCYIRTEPTPQIVWLKDNKLIPMDTLIQSKNSDSKNGIKKYENYLIHIRPGLYKAQLAVNDIRKYDFGSYSCQAANLQGEAQLEIHLSGTSTPDVPINFRLLNATSSTLHVAWTQGFDGGLAQTFQVRWREVGSISLYKYADVASNDNRNGVEYIITGLKPGSEYIVSVNSMNSKHGASAYTDPIHLRTLSLDLYNGRE---PMPPLSTSGYSDDNALLIIVSA 1330
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 145.591 bits (366), Expect = 1.937e-34 Identity = 191/832 (22.96%), Postives = 332/832 (39.90%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQP--NGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNEN---SALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVI-SYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIK--SVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQ--SSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYT--WFRNSTKLSRKNVHSISTSKA-KLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEI---IRQNAELECSVQSNPANPHM-SYKW 2756
Q F+ P+ + V G+ A L+C V G VQW +LLG R++PG P Y + G +G++ L+I +V ++D YECQV P++ R L V + P +P + +P G++ + TC++ + +P + + + + D E+ S ++L + + + + G + + + V PP P I + + ++ + C S G P +L W K E+ + +E D+ Q K S L + + +++GA L C + N V S QS L +L P +V + G E V G + T+ C T I+W +NN ++ E E+ S + ++++ + L C+A++ G + S++S+ L + Y P+K + G ++ + C + GGNP L W +++K +Q S + + G + + + PS DN Y C A + ++ + S ++ L+V FP +++ + + E+ G+ L L C AG SNP A + W L L+ +FGG + S + + H V C+ F+ E +NV F P+F I E + TL + ANP +D+T W + K+ + H + L I N D NY + N G+ I L++ Y P + K + + + A+L C +NP P M S+KW
Sbjct: 43 QAFRTEPRNL-TVRTGATALLKCEVLRASGSVQWVKDGLLLGPQRSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIPPAEPYFEWDAGKPWVEGEEYTV-----------TCIAPDAKPAADVLIYKDGDELTDIESFTESGSEDKLQNTHAEVKYRNVMIYIGLCLTALFLYLPPSLLLP-LLLRYLSLGLAVSEVPPQAPVIEGLRSQEVK---AGTSLRLVCLSY-GGNPLATLHWT----KNGEVLSTNWE----VDVVSQ---------KASSGLNM-QVQPEDNGAVLCCESVNQVSRSPQSITRTLTVLFEPAEVTLL--------------GSFEAV----EGNQVTLCCHTSSSNPPVHIRWWLGFK-ELNNATVTISEG---ENGGMMTMSNLTYTVSREENSLPLTCEAFNRGTRYSSIKSQ-PLSVYYPPQKVWLDAPPEGTLLRSGTTVRLVCFSSGGNPTGRLTWMKNNKTVQVPSKHVESERGVSREFLLTLQPS--DNLATYRCDATN---EAKEIRSAQIKLLVQFPAVSVKIL-VKQKELRAGRSLSLDCLAGSSNPKANISWSL-------------------GSTRLNGVEQAPKRAEFGGLSVSSKLTLPLGSEHHGKRVSCQAFSSLLSEGVNTF---YVLNVLFPPEFAEDQPSEVQIVEDDTATLPIMVSANP--EDFTCEWLFHGEKVVKDRDHRYHFPEGWALEIWNVTRRDAGNYRVECSNAEGKRSSIIKLDVQYSPTV---KMKTDPVFVNVGDTADLMCEADANPITPGMFSWKW 783 HSP 2 Score: 108.997 bits (271), Expect = 3.293e-23 Identity = 73/235 (31.06%), Postives = 117/235 (49.79%), Query Frame = 3
Query: 2952 KSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIR--GDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNS 3641
+S+ T LL +Y+V R G Y+C +N I G VV +L F P++ P++ K A G+ +V + C P TW R N +Y R + + L +VN+ D+ + C A N +G D+ I++ TS PD P ++++++ + + + W+ GFDGG IQ F+VR++ S Y Y DV + + + +TGLK TAY S+N+ NS
Sbjct: 795 QSEDGATGLLTLYEVTRAQAGPYQCTADNEIAPPGSVVG--QLVVRFAPELQKGPQWRKVASRGDGSTDVDVLCQAEGVPRVTFTWAKNRVPLDF-PNPRYLERTVR-EGAIHTSTLTVVNVSAALDYAIFTCTARNSQGEDNLDIQLLSTSYPDPPSDLKLLSVASDSVTLEWMPGFDGGLIQNFRVRYQWAESAS-YLYVDVFPPRSTV---YTVTGLKPVTAYNFSVNAINS 1021 HSP 3 Score: 63.5438 bits (153), Expect = 2.174e-9 Identity = 128/613 (20.88%), Postives = 223/613 (36.38%), Query Frame = 3
Query: 1692 PEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQF-------EGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRN--STKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGE-SQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKW----TKL-----VPGK---GYNDISSSDLVP-------------AFTI----GCNQ----SLPFSKEYKNEKPKPYSKAE---------WKGTPEK--SNFKYFSKCMVKSQ-----FEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHP-SPKM-TWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAEN 3347
P+ P I+G + VK + + C + GGNP L W ++ + + +++ V + + + + DN C+ S++ S S TL VLF P + L L E G + L C SNPP +RW L EL V + G+ GG + S S +++ + CE FN G + ++ + ++V + PQ EG + + G + L P TW +N + ++ K+V S + ++ ++ Y N+ E I L + + + + ++KE +R L + +NP + W T+L P + G +SS +P AF+ G N ++ F E+ ++P E PE + + + +VK + F L I++V R D G YR +N G I+L +++P + K N+G+ L C ++P +P M +W + + T L + + R G Y C A+N
Sbjct: 255 PQAPVIEGLR-SQEVKAGTSLRLVCLSYGGNPLATLHWTKNGEVLSTNWEVDVVSQKASSGLNMQVQPEDNGAVLCCE--SVNQVSRSPQSITRTLTVLFEPAEVTL--LGSFEAVEGNQVTLCCHTSSSNPPVHIRWWLG-----FKELNNATVTIS--------------EGENGGMMTMSNLTYTVSREENSLPLTCEAFN--RGTRYSSIKSQ-PLSVYYPPQKVWLDAPPEGTL-----LRSGTTVRLVCFSSGGNPTGRLTWMKNNKTVQVPSKHVESERGVSREFLLTLQPSDNLATYRCDATNEAKEIRSAQIKLLVQFPAVSVKILVKQKE-LRAGRSLSLDCLAGSSNPKANISWSLGSTRLNGVEQAPKRAEFGGLSVSSKLTLPLGSEHHGKRVSCQAFSSLLSEGVNTFYVLNVLFPPEFAEDQPSEVQIVEDDTATLPIMVSANPEDFTCEWLFHGEKVVKDRDHRYHFPEGWALEIWNVTRRDAGNYRVECSNA-EGKRSSIIKLDVQYSPTVKM--KTDPVFVNVGDTADLMCEADANPITPGMFSWKWMGEGEVEELGEQSEDGATGL--------LTLYEVTRAQAGPYQCTADN 823
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: cadm2b (cell adhesion molecule 2b [Source:ZFIN;Acc:ZDB-GENE-080506-3]) HSP 1 Score: 73.1738 bits (178), Expect = 9.011e-13 Identity = 74/304 (24.34%), Postives = 131/304 (43.09%), Query Frame = 3
Query: 1347 ISVSDGSLSSRTIGKDG---LNENVIYGEAGEEKTITCTTDPYYNEATIKW--------VFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSIN--DNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKF 2219
+VS+ + ++ G G + +NV E G +TC D Y + +++W F A+ ++ I V WKE TI I D+ C ++ + S+ L + PEKP I G++ +P + +TC T G P +++WFR+ K +Q G T++ + ++ D+ Y C + ++L S+P EV L V + P + SL + G+ KL+C + + P V W + G + ++ R V+ EL+ + + T NG +
Sbjct: 13 FTVSEAATKNKAKGSQGQFPITQNVTVVE-GSTANMTCRVD-YNDNTSLQWSNPAQQTLFFGDKKALRDNRIELVRASWKE-------LTISISEVTLSDEGQYTCSLFT-MPVKTSKAFLTVLGVPEKPEIIGFS--RPAVEGEDVTLTCITTGSKPAADIRWFRNDKEVQGVKEVNATGRSFTVRSAVHLRVDRKDDGAAYTCSVEHVALNSTPHQVTEV-LEVHYAPKVEIVHSL--IVPQEGQYFKLECVSKGNPLPEPVVW--TKDGGELPDVERMIVE---GRELIITTLNKTDNGTY 296
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: ENSAMXT00000045550.1 (immunoglobulin superfamily member 9B [Source:NCBI gene;Acc:103022418]) HSP 1 Score: 73.559 bits (179), Expect = 1.283e-12 Identity = 112/447 (25.06%), Postives = 162/447 (36.24%), Query Frame = 3
Query: 2466 YTWFRNSTKLSRKNVHSISTS---KAKLIIRNAKPVDIDNYTLVVVNDRGES-QITIYLNITY------QPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGN-----LGEEVSLFCYIRSHPS-PKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGS--DSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKL 3752
YTWF + KN + LII KP D YT N G S + YL I Y P +I + +IR C V +NP P S +W K GY P E W P+ S + + +V + +G Y CV N + T+ + T + P F+ G G E+ + C P P +TW V S+ + NI L+ V+L + DHG + C A N S S ++ V GTS P P + V + S + +SW G+DGG QTF V + PT L ++ ++ ++ GL+ TAY S+ ++N K GT FS+ + NT+ YP+ P L
Sbjct: 261 YTWFWEEDNVYFKNDLKLRVRILIDGTLIIFRVKPEDAGKYTCSPSNSLGISPSSSAYLTIQYPARVLNMPPVIYVPRKLPGVIR------CPVDANP--PVTSVRWEK----DGY--------------------PLRVE---------KYPGWSQMPDGS-------------------IRVAEVTEDSLGTYTCVPYNAL-----GTMGQSPPATLVLKDPPYFNVRPGGEYRQEAGRELVIPCAASGDPEIPTITW------RKVGKPSRSKHNI------LPSGSLQFVSLSKEDHGEWECVATNVVTSITASTRLFVIGTS-PHAPANVHV-SASTTSANVSWEPGYDGGFEQTFSVWY---GPTDLGPHDWLSVPVPGSQTWLVVQGLEPKTAYQFSVLAQN-KLGTGPFSEVVTVNTV-----------VYPVSTPEPL 613
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: ENSAMXT00000003312.2 (immunoglobulin superfamily member 9B [Source:NCBI gene;Acc:103022418]) HSP 1 Score: 74.3294 bits (181), Expect = 1.283e-12 Identity = 113/450 (25.11%), Postives = 166/450 (36.89%), Query Frame = 3
Query: 2466 YTWFRNSTKLSRKNVHSISTS---KAKLIIRNAKPVDIDNYTLVVVNDRGES-QITIYLNITY------QPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGN-----LGEEVSLFCYIRSHPS-PKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGS--DSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRW---KEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKL 3752
YTWF + KN + LII KP D YT N G S + YL I Y P +I + +IR C V +NP P S +W K GY + EK W P+ S + + +V + +G Y CV N + T+ + T + P F+ G G E+ + C P P +TW V S+ + NI L+ V+L + DHG + C A N S S ++ V GTS P P + V + S + +SW G+DGG QTF V + K+K+ + + V ++ L ++ GL+ TAY S+ ++N K GT FS+ + NT+ YP+ P L
Sbjct: 261 YTWFWEEDNVYFKNDLKLRVRILIDGTLIIFRVKPEDAGKYTCSPSNSLGISPSSSAYLTIQYPARVLNMPPVIYVPRKLPGVIR------CPVDANP--PVTSVRWEK----DGY------------------------PLRVEK-----YPGWSQMPDGS-------------------IRVAEVTEDSLGTYTCVPYNAL-----GTMGQSPPATLVLKDPPYFNVRPGGEYRQEAGRELVIPCAASGDPEIPTITW------RKVGKPSRSKHNI------LPSGSLQFVSLSKEDHGEWECVATNVVTSITASTRLFVIGTS-PHAPANVHV-SASTTSANVSWEPGYDGGFEQTFSVWYGPVKKKTDLGPHDWLSVPVPGSQTWL--VVQGLEPKTAYQFSVLAQN-KLGTGPFSEVVTVNTV-----------VYPVSTPEPL 617
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: ENSAMXT00000055476.1 (immunoglobulin superfamily member 9B [Source:NCBI gene;Acc:103022418]) HSP 1 Score: 74.3294 bits (181), Expect = 1.311e-12 Identity = 113/450 (25.11%), Postives = 166/450 (36.89%), Query Frame = 3
Query: 2466 YTWFRNSTKLSRKNVHSISTS---KAKLIIRNAKPVDIDNYTLVVVNDRGES-QITIYLNITY------QPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGN-----LGEEVSLFCYIRSHPS-PKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGS--DSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRW---KEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKL 3752
YTWF + KN + LII KP D YT N G S + YL I Y P +I + +IR C V +NP P S +W K GY + EK W P+ S + + +V + +G Y CV N + T+ + T + P F+ G G E+ + C P P +TW V S+ + NI L+ V+L + DHG + C A N S S ++ V GTS P P + V + S + +SW G+DGG QTF V + K+K+ + + V ++ L ++ GL+ TAY S+ ++N K GT FS+ + NT+ YP+ P L
Sbjct: 261 YTWFWEEDNVYFKNDLKLRVRILIDGTLIIFRVKPEDAGKYTCSPSNSLGISPSSSAYLTIQYPARVLNMPPVIYVPRKLPGVIR------CPVDANP--PVTSVRWEK----DGY------------------------PLRVEK-----YPGWSQMPDGS-------------------IRVAEVTEDSLGTYTCVPYNAL-----GTMGQSPPATLVLKDPPYFNVRPGGEYRQEAGRELVIPCAASGDPEIPTITW------RKVGKPSRSKHNI------LPSGSLQFVSLSKEDHGEWECVATNVVTSITASTRLFVIGTS-PHAPANVHV-SASTTSANVSWEPGYDGGFEQTFSVWYGPVKKKTDLGPHDWLSVPVPGSQTWL--VVQGLEPKTAYQFSVLAQN-KLGTGPFSEVVTVNTV-----------VYPVSTPEPL 617
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008186.1 (pep scaffold:Pmarinus_7.0:GL476501:83598:168579:1 gene:ENSPMAG00000007342.1 transcript:ENSPMAT00000008186.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.1738 bits (178), Expect = 6.493e-13 Identity = 71/271 (26.20%), Postives = 119/271 (43.91%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTG-FYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGS-IQTFQVRWKEKSPT--SLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIV 3779
L++ V D G Y C V+NG+ D+ K++ L + + S + A G+ +L C R + W + T + +R + TG ++ L+I+ R D G + C A N G D I+++ PD P E++V + I + W GFDG S I F + +K +S T R +D++S + T I L T Y+ + ++N + G S+ SK + T A + P I++P Q + + V
Sbjct: 585 LLLRQVQEEDGGFYLCQVSNGVGTDISKSMLLNVKIPALVTSQANVTLA--TRGQHSNLQCSARGERPIIIRWE--KEETLIDPERMFRYVVYTQDTGDELLSTLQILPTVRGDSGFFTCHAINSYGEDRGLIQLTVQEPPDPP-EVEVREVNARTITLRWTSGFDGNSPITAFDIEYKNRSDTWEQKQRTKDISSTINQAT----IIDLHPATHYVFRVYAKN-RIGASEPSKDLPITTQEA---APDGPPLEVILEPQSSQSIRVTWKV 842 HSP 2 Score: 56.6102 bits (135), Expect = 6.818e-8 Identity = 52/221 (23.53%), Postives = 101/221 (45.70%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRCVVNNGIRGDVVK--TIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDV-PLE-IQVVNKSQSQIEISWIQGFDGGSIQTFQVRW-KEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISI 3626
L ++ R+D G Y+CV G GD + +I + ++ +P+I S FS+ + GE SL C +R P P + W ++S ++ +R G L + + TD G Y C+A N G + ++ +P + P+ + + ++ + I G+ + +RW +E +P + V ++ T + L+ + G+ + AY+ S+
Sbjct: 202 LSVHAAGRSDGGTYQCVAR-GEGGDTAQDFSILMLEDGSPKIAS--SFSERVVSPGEPFSLQCVVRGSPPPTIAWTLDG-EPALSRDAPHRRGNHIVTPGDVTGYLNVTGARVTDGGVYRCQAGNAAGQAEYQARINVRGSPSIRPMRNVTAIAGREAYLHCRVI-GYP-----YYSIRWLREGTPLPYNHRQTVFANGT-LRLADVQKGMD-EGAYICSV 410
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002463.1 (pep scaffold:Pmarinus_7.0:GL476400:5068:120393:-1 gene:ENSPMAG00000002229.1 transcript:ENSPMAT00000002463.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 5.784e-9 Identity = 64/234 (27.35%), Postives = 103/234 (44.02%), Query Frame = 3
Query: 2979 LIIYDVDRNDVGLYRC-VVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNL----GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWI-QGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQ 3659
L+I +V +D +Y+ N G G + T L E P ++LPK + G + GE +S+ P P +TW G+ + N KY++ +TR T ++R+D G YV A+N G D + +E+ PD P ++V ++ + + W DGGS I + V EK T+ R+ V + + + L T+Y I N K G S+
Sbjct: 16853 LVIAEVTEDDSTVYQVRATNQG--GSISATASLDVEV-PAKINLPKHLEGMGAVHALRGEVISIKIPFSGKPDPVITWQKGQ--DLLDNNGKYQVIVTRSFTSLVFTN----GVERSDAGFYVVCAKNRFGIDQQTVELDVADVPDPPRGLKVSEVTRDSVSLEWAPPANDGGSRITNYIV---EKCATTADRWLRVVATR---ECRYTVINLFGKTSYQFRIIGEN-KFGQSK 17070 HSP 2 Score: 53.1434 bits (126), Expect = 8.289e-7 Identity = 48/180 (26.67%), Postives = 81/180 (45.00%), Query Frame = 3
Query: 3138 GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISW-IQGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKT 3671
G + LF IR HP P++ W A+ + R N T Y L I N+ R D G Y+ EN GS S + V TP P +++ ++ + ++W DGG+ ++ + + EK ++ Y VA++ K S+ I L+ Y +++ N +HG ++T
Sbjct: 8861 GNSLRLFIPIRGHPPPEVKW--------TKADGEIRENAQIDTTDSYTL-LVIDNVNRFDSGKYIITGENASGSKSAFVTVRVLDTPSPPEHLKIREITKDSVTLTWEPPAIDGGAKVKNYII---EKRESTRKAYAAVATNCHK--SSWKIDQLQEGCNYYFRVSAEN-EHGIGLPAET 9025 HSP 3 Score: 51.9878 bits (123), Expect = 1.901e-6 Identity = 52/191 (27.23%), Postives = 84/191 (43.98%), Query Frame = 3
Query: 3138 GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGF-DGGS-IQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNE 3704
G + ++ IR P PK+TW +++N K RI + + Y L N+ + D G Y+ EN G + I V TP P+ + V ++ SW DGGS ++++ V EK + V +D TK SF IT L+ +Y + N ++G + +T T+ + A E
Sbjct: 5611 GVTMRIYVPIRGRPPPKITW------AKMNSNIKDRIGLD-IKNTDYDTYLHCENVNKYDAGKYILTLENSSGIKTYTINVKVLDTPGPPVMLSVKETTKESAVFSWDPPLIDGGSPVKSYIV---EKREAERKAWSTVKTDCTKT--SFRITNLEEGKSYFFRALAEN-EYGIGEPGET--TDAVKASEE 5786
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Match: igdcc3 (immunoglobulin superfamily, DCC subclass, member 3 [Source:ZFIN;Acc:ZDB-GENE-080303-24]) HSP 1 Score: 54.6842 bits (130), Expect = 1.893e-7 Identity = 61/252 (24.21%), Postives = 102/252 (40.48%), Query Frame = 3
Query: 2958 QFEMTSLLIIYDVDRNDVGLYRCVVNN-GIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKG--SDSRKIEVSGT-STPDVPLEIQVVNKSQSQIEISW---IQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMS 3692
Q T L+I DV G+Y C N G R L + P+ + P+ +LG L C + PSP++ W + + + R+ R + G + L I + D G Y C AEN G S ++ V G+ P P +++V S + +E++W +Q G +R + S Y++ S + T+ + L+ T Y + + +S G SQ S + T+
Sbjct: 247 QVVGTGNLMISDVAVQHAGVYVCAANRPGTRVRRTAQGRLIVQAPPEFMQPPQ--SVTRSLGASAVLTCSAQGTPSPRIIWL----HNGLPLHQSERV---RLQNGN--STLTIQRVCSEDEGIYQCTAENSAGYTQASARLAVLGSDGLPGPPWDLRVRATSATAVELAWREPLQNPHGVIGYVLHIRRAQDSEDQ--EYQEAVS---QTTVQHTVLELEPSTHYTFYVKAYSSG-GASQPSDYVTAETLG 481
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Match: ncam1b (neural cell adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-010822-2]) HSP 1 Score: 52.373 bits (124), Expect = 1.056e-6 Identity = 56/242 (23.14%), Postives = 105/242 (43.39%), Query Frame = 3
Query: 2961 FEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQ-GFDGG-SIQTFQVRWKEKSPTSLY-RYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIM 3677
+ S L + DV D G Y C+ NN + G + L + P+ + N V++ C + ++P ++W R + + + + + F GF V L+I ++D GSY C A N S+S++ + + P P ++ + EI+ + + GG I T+ VR + + S + Y S+ + +T L+ +T Y+ +++RN G F+K +
Sbjct: 251 YAKVSSLTLRDVQYTDAGEYMCIANNNV-GQASAAMHLDVAYGPKFAGEKVTYHSWPN--NAVNVTCEVLANPKAAVSW--SRDGQPIPSANLTNVIVHSF-PGFSV--LEITPESQSDFGSYNCVAVNNIASESKEFVLVEAALPSAPQSVKATAVYSTSAEITLEEPDYTGGVPILTYTVRVRPANGLSEWTSYYFGGSEIWNYENTVKVTNLEPNTQYMAEVSARNGV-GDGPFTKPTL 483
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002502.1 (pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-1 gene:ENSPMAG00000002253.1 transcript:ENSPMAT00000002502.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 1.504e-6 Identity = 88/391 (22.51%), Postives = 144/391 (36.83%), Query Frame = 3
Query: 2397 FYIEEGQSQTLNLE-PIANPPIKDYTWFRN----STKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQ--PIIIGDKFERKEIIRQNAEL-ECSVQSNPANPHMSYKWTK-LVPGKGY------------------NDISSSDLVPAFTIG---CNQSLPFSKEYKN-----EKPKPYS-------------------KAEW----KGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNL----GEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVS 3383
+++ + ++ LE +A P+ +WF++ ++ L+RK V ++ A L +RN + + Y ND G + L +T + P + K + + + A + +C P +K K + PG+ Y ND + +G C SL KE KN ++ P S K W K +N+K+ S + T LL I D+ D G+Y C N D KE P F + +L GE L C++ K+TW + N + A YR+ +A+L I+ + + D G Y C A N GSD E++
Sbjct: 5968 LFVKVSEPGSVRLECEVAGTPVIKTSWFKDDVLITSGLNRKPVQKLNL--AILELRNVQYSESGEYVCKAENDIGSATSVAMLTVTERKNPPVFTRKLKDVQGVEGGAAMFDCRATGTPPFAVAWFKHGKEITPGEKYRMSFEENVVFLEILNLATNDEEEYSCTVSNDVGKISCAASLEV-KERKNPPSFDQQLAPASVTAGEPADLGCHVTGSPPFKISWLKDNKEIRPGTNYKF-------SFVDNTPLLTILKTDKRDSGVYVCRAANEAGYDTSCAPVTIKERR----VAPTFDQKLESLNVTTGERAELECHVTGSVPIKVTW--AKDNREIRAAGNYRMIFME-----NIARLTILKVGKLDSGIYTCAASNDVGSDVTSTEIT 6337
BLAST of NPHS1-6 vs. Ensembl Nematostella
Match: EDO27477 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T954]) HSP 1 Score: 60.8474 bits (146), Expect = 2.232e-10 Identity = 41/141 (29.08%), Postives = 65/141 (46.10%), Query Frame = 3
Query: 2973 SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTST 3395
S+L +Y+V R + G Y C +NGIR + L+ F P+I K +LG+ ++L C + P P +TW R S++ I + R + L+++ Q D G Y+C A N G DS + + T
Sbjct: 48 SILTLYNVTRREAGSYECKADNGIRKPAKASAVLSVNFAPEI-ERQKKKHFETSLGKAINLTCDVEGGPLPSVTWSRKR--------SQFSIIFSPVR---MRSVLEVIPKQSRDFGDYICMARNIMGWDSHTLTLKRVGT 176
BLAST of NPHS1-6 vs. Ensembl Nematostella
Match: EDO31942 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWF5]) HSP 1 Score: 63.5438 bits (153), Expect = 5.920e-10 Identity = 66/250 (26.40%), Postives = 107/250 (42.80%), Query Frame = 3
Query: 2970 TSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWI-QGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKE 3710
TS+ V R++ G Y C N + D T+++T + P++ S + + + +SL C R P P++TW+ ++ G QL++ Q + D+G Y C+A N GS R I V+ +P P+ I V N S I+++W DGGS + ++V P E VA +I+GL +T Y + + +RN + I T A+NE E
Sbjct: 222 TSVYYSDSVTRSEAGTYTCTATNEMGSDT-NTVQVTVNYKPEMASSTTNTSSWNR--KVLSLQCKARGVPLPQITWYK-------PGGQPITAGVSPISGG----QLQVTTTQDSGDYGQYKCRASNLLGSVGRLIYVTQWFSPGPPI-IIVSNIEASSIKVNWAPPSSDGGSPVIDYKVEVDPAKPPR----EGVAGGP------VVISGLTKNTEYTVKVTARNVVGYGEAGTIIIQTRKQDANNESE 446
BLAST of NPHS1-6 vs. Ensembl Nematostella
Match: EDO43516 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYG4]) HSP 1 Score: 61.6178 bits (148), Expect = 2.294e-9 Identity = 64/270 (23.70%), Postives = 111/270 (41.11%), Query Frame = 3
Query: 2973 SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQ--PNKLQDNTILFI 3776
S + I DV D G Y C N R I T+E I SLP+ +K G ++++ Y+ +PK + R++ N + + G A I+ + D G Y+C + N GS + ++++ P P+ ++ N + S++ ++W D G ++ Q + Y+ + + IT LK DT Y I I +RN +T+ T ++ P+ PII K+ D+T F+
Sbjct: 378 SRIYIQDVRLEDEGRYTCEARNNARDQEGHPIVATEEIGLYIKSLPRLNKK----GTPLTVYSYLG---NPKPVFIRCRFDGYPFPNVTIWFDNKQIANGTEFAMFDIITNELEDFGDYLCWSWNIHGSKNVTVKLTHAREPSRPVNVK-ANVTCSRVTLTWDPPIDDGGMEIMQY---------VIEYDGKIFNTEDTYPKYYITNLKRDTMYDIKIWARNKMGKGKNNYETVSIIT------QKYCPPSRPIIWFPYEKVIDDTAFFL 624
BLAST of NPHS1-6 vs. Ensembl Nematostella
Match: EDO26116 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCU5]) HSP 1 Score: 58.151 bits (139), Expect = 9.603e-9 Identity = 57/209 (27.27%), Postives = 89/209 (42.58%), Query Frame = 3
Query: 3153 LFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVN---LQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWI-QGFDGGS-IQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNT 3764
L C +P+P TW R G V+Q + N + R++ G+Y C A N GSD+ ++V+ P P+ + V N S I+++W DGGS + ++V E P R E VA D +I+ L +T Y + + +RN +KTI T K+ P+ P+++P DNT
Sbjct: 31 LTCESDGYPAPTYTWS---------------------RDGVVVSQTSVYNSDSVTRSEAGTYTCTATNEMGSDTTTVQVTVNFAPGPPI-VTVSNIEASSIKVNWAPPSSDGGSPVIDYKV---EVDPAKPPR-EGVAGDQ------VVISDLTKNTEYTVKVTARNVVGYGEAGTKTIRT--------KKQGPPSLPVLKPG---DNT 196
BLAST of NPHS1-6 vs. Ensembl Nematostella
Match: EDO45383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT26]) HSP 1 Score: 59.3066 bits (142), Expect = 9.836e-9 Identity = 43/152 (28.29%), Postives = 68/152 (44.74%), Query Frame = 3
Query: 2973 SLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWF--FGRYNTSVS----ANSKYRI--NITRFRTGFYVAQ----------------LKIVNLQRTDHGSYVCKAENGKG 3356
++L+I +V R D G Y C +N + G + L + P+ +++P+ N G V+ C+ + +P P +TW NTSV +N + + N+ R +G Y L I NL RTD SY C+A+N G
Sbjct: 50 TILMITNVSRIDSGTYSCNASNEL-GIISAEAALDVRYGPEFVNVPQ--DKTPNEGWSVTFECHTKGNPPPTVTWLKNGSALNTSVDPRFYSNGTHLMITNVNRTDSGAYRCNASSILGPENDGNETSFLMISNLNRTDEASYTCRAKNAAG 198
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 159.073 bits (401), Expect = 1.068e-38 Identity = 194/825 (23.52%), Postives = 342/825 (41.45%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALL---EELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSR-VKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSS--FIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEV-ENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWF-RNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHM-SYKW 2756
Q F+ P+ + V G+ A L+C V G VQW +LLG R++PG P Y ++G +G++ L I N ++D YECQ ++ L VL+ P P F + + DV TCV+ + +P+ + + + + E+S++ ++L + + +++ +G ++ C N A + + T V + ++ PP P IV+++ + V ++M S SG P +L W K E+ + +E + ++ +++ S KMV +T ++ A+L C + N V +S S KL + P ++ +S GSL + G+E ++C I+W N FI + ++ S + + +++ + L+C+A++ G + + + K+ + Y P+K + P+ + C + GGNP P L W ++ K + + + + + L +++TPS DN Y C A + + K+ S L+V F + +K + EV G++L L+C A SNP A + W + +E QT + K G + S + + + H V C+ +NP E + +NV P+F +EE + T+ L ANP W R T + N+H L IRNA D YT+ N+ G SQ I L++ Y + +K + A+L C +NP M S+KW
Sbjct: 27 QAFRIEPRNL-TVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGK--RGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPY-----FTMDMAAYWVAGESYDV----TCVAPDAKPEAEVILYKDGVKLTGVESSSVSGSKDKLLNTLAEVTVTALSSDNGRLLACHAKNAALS-------KPVVTTVTMNVYYPPDAPFIVNLDRDE----VRAGRMLMLECVSGSGNPLAALRWS----KNGEIVSTTWE-------ADEFARKSRSVLKMV-------ITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLS---GSLEAI---------------EGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFI----REEGDNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPS--DNMATYRCDATNNAKKTI---SAHAKLLVYF--TAVSMKITAHQEVLRQGEVLTLECLAESSNPTANIFWSVGAVRFQKAE------QTHSKD-------------KSGAVTVNSTLLLNLTSQHHKQNVICQAYNPVLEEGANTYYR---LNVLHPPEFSSAQPTEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGNNLHHYWLEDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPIISDMFSWKW 759 HSP 2 Score: 103.99 bits (258), Expect = 9.652e-22 Identity = 70/233 (30.04%), Postives = 118/233 (50.64%), Query Frame = 3
Query: 2955 SQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVS-ANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNS 3641
+Q + +SLL I +V R GLY+C NNGI ++L +F P++ P++ K A G+ L C R+ P++ + + + + AN +Y R F+ + L++VN+ D+ + C A N G D I++ T+ PD P + V+ + + + WI GF+GG Q F++R++ S ++Y DV D +F +TGL+ T Y SIN+ N+
Sbjct: 771 TQEDESSLLTIRNVTRAHSGLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVC--RAEGIPRIDFSWEKKGVFLDLANPRYEEKTVR-EGSFHTSTLRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWDQSVS-FQYVDVFPPDAT---TFTVTGLQPVTVYNFSINALNA 996
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 150.599 bits (379), Expect = 5.210e-36 Identity = 192/826 (23.24%), Postives = 332/826 (40.19%), Query Frame = 3
Query: 315 QCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQW---FLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPV-ISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWS-GIKSVQSR-VKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSS--FIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEV-ENGKLLKLQCTAGESNPPAVVRWKLN--RCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHM-SYKW 2756
Q F+ P+ + V G+ A L+C V G VQW +LLG R++PG P Y ++G +G++ L I N ++D YECQ ++ L VL+ P P F + + DV TCV+ + +P+ + + F + + +AL + +G ++ C N A + + T V + ++ PP P IV+++ + V ++M S SG P +L W K E+ + +E + ++ +++ S KMV +T ++ A+L C + N V +S S KL + P ++ +S GSL + G+E ++C I+W N FI + ++ S + + +++ + L+C+A++ G + + + K+ + Y P+K + P+ + C + GGNP P L W ++ K + + + + + L +++TPS DN Y C A + + K+ S L+V F + +K + EV G++L L+C A SNP A + W + R G +S + T + LL+ +S + H V C+ +NP E + +NV P+F +EE + T+ L ANP W L + + L IRNA D YT+ N+ G SQ I L++ Y + +K + A+L C +NP M S+KW
Sbjct: 50 QAFRIEPRNL-TVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGK--RGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPY-----FTMDMAAYWVAGESYDV----TCVAPDAKPEAEHFFVCIFTFGSCVTSERVTALSSD----------------NGRLLACHAKNAALS-------KPVVTTVTMNVYYPPDAPFIVNLDRDE----VRAGRMLMLECVSGSGNPLAALRWS----KNGEIVSTTWE-------ADEFARKSRSVLKMV-------ITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLS---GSLEAI---------------EGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFI----REEGDNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPS--DNMATYRCDATNNAKKTI---SAHAKLLVYF--TAVSMKITAHQEVLRQGEVLTLECLAESSNPTANIFWSVGAVRFGVTLSTKHQSGAVTVNSTLLLNLTS-----------------------QHHKQNVICQAYNPVLEEGANTYYR---LNVLHPPEFSSAQPTEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGD------PDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPIISDMFSWKW 757 HSP 2 Score: 104.375 bits (259), Expect = 6.749e-22 Identity = 70/234 (29.91%), Postives = 119/234 (50.85%), Query Frame = 3
Query: 2952 KSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAA--GNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVS-ANSKYRINITRFRTGFYVAQLKIVNLQRT-DHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNS 3641
++Q + +SLL I +V R GLY+C NNGI ++L +F P++ P++ K A G+ L C R+ P++ + + + + AN +Y R F+ + L++VN+ D+ + C A N G D I++ T+ PD P + V+ + + + WI GF+GG Q F++R++ S ++Y DV D +F +TGL+ T Y SIN+ N+
Sbjct: 768 ETQEDESSLLTIRNVTRAHSGLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVC--RAEGIPRIDFSWEKKGVFLDLANPRYEEKTVR-EGSFHTSTLRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWDQSVS-FQYVDVFPPDAT---TFTVTGLQPVTVYNFSINALNA 994
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: ncam1a (neural cell adhesion molecule 1 [Source:NCBI gene;Acc:101173164]) HSP 1 Score: 78.9518 bits (193), Expect = 3.444e-14 Identity = 94/399 (23.56%), Postives = 166/399 (41.60%), Query Frame = 3
Query: 2469 TWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAE--LECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPK---FSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVN--KSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSK 3644
TW N+ L + +S++ ++LII++ VD +YT + N GE + LN+ QP I K I + E L C +P P +S+ S S + FT G +A W P+K ++ + +V S L + +V G Y C+ +N I D +++ L ++P+I + + K NL C + +HP + WF + + + + + + I T V+ L+I + D G+Y C A N GS+S++ + P P EIQ V S + +E G + ++V W + P + ++ + +D K + ++TGLK +T Y + +++ N K
Sbjct: 180 TWTHNNVVLEEGDKYSLNEDGSELIIKDVMKVDEGDYTCIAKNKAGEKSQEVSLNVFVQPKIT--YLNNKTAIEFDEEVILTCEASGDP-TPTISWS-------------SESKI---FTEG-------------------EQASWT-RPDK--YESLDRNIVVYSHARVSSLTLKNVQFTYAGQYLCIASNSIGQDS-QSVYLEVRYSPKIQGVVASYTWEKNPANLS------CEVDAHPEASVVWF--KDGSHLPSPNTTNVKIYNMPT---VSYLEITPESQIDFGNYNCTATNDLGSESKEFILIQADVPSAP-EIQQVEPFSSTAMVEFEEPDSTGGVPVLKYKVEW--RFPGEEWARKEYSKEDGKTKI--LVTGLKPETTYEVKMSAINGK 520
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: ncam1a (neural cell adhesion molecule 1 [Source:NCBI gene;Acc:101173164]) HSP 1 Score: 78.5666 bits (192), Expect = 4.288e-14 Identity = 94/399 (23.56%), Postives = 166/399 (41.60%), Query Frame = 3
Query: 2469 TWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAE--LECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPK---FSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVN--KSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSK 3644
TW N+ L + +S++ ++LII++ VD +YT + N GE + LN+ QP I K I + E L C +P P +S+ S S + FT G +A W P+K ++ + +V S L + +V G Y C+ +N I D +++ L ++P+I + + K NL C + +HP + WF + + + + + + I T V+ L+I + D G+Y C A N GS+S++ + P P EIQ V S + +E G + ++V W + P + ++ + +D K + ++TGLK +T Y + +++ N K
Sbjct: 180 TWTHNNVVLEEGDKYSLNEDGSELIIKDVMKVDEGDYTCIAKNKAGEKSQEVSLNVFVQPKIT--YLNNKTAIEFDEEVILTCEASGDP-TPTISWS-------------SESKI---FTEG-------------------EQASWT-RPDK--YESLDRNIVVYSHARVSSLTLKNVQFTYAGQYLCIASNSIGQDS-QSVYLEVRYSPKIQGVVASYTWEKNPANLS------CEVDAHPEASVVWF--KDGSHLPSPNTTNVKIYNMPT---VSYLEITPESQIDFGNYNCTATNDLGSESKEFILIQADVPSAP-EIQQVEPFSSTAMVEFEEPDSTGGVPVLKYKVEW--RFPGEEWARKEYSKEDGKTKI--LVTGLKPETTYEVKMSAINGK 520
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: ncam1a (neural cell adhesion molecule 1 [Source:NCBI gene;Acc:101173164]) HSP 1 Score: 78.5666 bits (192), Expect = 4.352e-14 Identity = 94/399 (23.56%), Postives = 166/399 (41.60%), Query Frame = 3
Query: 2469 TWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAE--LECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPK---FSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVN--KSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSK 3644
TW N+ L + +S++ ++LII++ VD +YT + N GE + LN+ QP I K I + E L C +P P +S+ S S + FT G +A W P+K ++ + +V S L + +V G Y C+ +N I D +++ L ++P+I + + K NL C + +HP + WF + + + + + + I T V+ L+I + D G+Y C A N GS+S++ + P P EIQ V S + +E G + ++V W + P + ++ + +D K + ++TGLK +T Y + +++ N K
Sbjct: 180 TWTHNNVVLEEGDKYSLNEDGSELIIKDVMKVDEGDYTCIAKNKAGEKSQEVSLNVFVQPKIT--YLNNKTAIEFDEEVILTCEASGDP-TPTISWS-------------SESKI---FTEG-------------------EQASWT-RPDK--YESLDRNIVVYSHARVSSLTLKNVQFTYAGQYLCIASNSIGQDS-QSVYLEVRYSPKIQGVVASYTWEKNPANLS------CEVDAHPEASVVWF--KDGSHLPSPNTTNVKIYNMPT---VSYLEITPESQIDFGNYNCTATNDLGSESKEFILIQADVPSAP-EIQQVEPFSSTAMVEFEEPDSTGGVPVLKYKVEW--RFPGEEWARKEYSKEDGKTKI--LVTGLKPETTYEVKMSAINGK 520
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 3256.46 bits (8442), Expect = 0.000e+0 Identity = 1670/1670 (100.00%), Postives = 1670/1670 (100.00%), Query Frame = 3
Query: 126 MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASXXXXXXXXXXXXXXXXXXXXXXXXXXTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIAXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXXXXXXXXXXXXXPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 5135
MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII
Sbjct: 1 MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 1670
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 3131.66 bits (8118), Expect = 0.000e+0 Identity = 1624/1666 (97.48%), Postives = 1625/1666 (97.54%), Query Frame = 3
Query: 159 FFLVLILI-------GSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASXXXXXXXXXXXXXXXXXXXXXXXXXXTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIAXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXXXXXXXXXXXXXPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 5135
F LVL LI G P + DD DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQ AEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII
Sbjct: 6 FLLVLCLIFELDDLYGKITHLSTP--TVDD----DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQ------------------AEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 1647
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 3131.27 bits (8117), Expect = 0.000e+0 Identity = 1614/1632 (98.90%), Postives = 1614/1632 (98.90%), Query Frame = 3
Query: 240 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASXXXXXXXXXXXXXXXXXXXXXXXXXXTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIAXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXXXXXXXXXXXXXPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 5135
DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQ AEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII
Sbjct: 15 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQ------------------AEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDMVELYPRGLDSSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKGSIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQDTMSTLLSPIHEGGQRQLMTFMSMIPINGQRNSSSCGSVHSGGNYSNTPQQIITSNISPAGSIIASDSHNQNNSSSDNVNINSGSQNGNFDQNQINTNNLNTYNNSSTNPTPYRSRVVSPPLMSYNIAPQHREAYANELRKAQLSGNILVNPTMFNRQSATSPHQLIGNPPLISPHIINSSGQSISMPISFENPSLNFSSINNCHGTMRNNDNHYNSLLVPTTLEGKVSQKYRCVDNDTNSSQSPNADRSIEFINPAMEDSLHQNNHSDSQYLPRPNII 1628
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000056135.1 (SMESG000056135.1) HSP 1 Score: 446.817 bits (1148), Expect = 1.173e-132 Identity = 341/1155 (29.52%), Postives = 549/1155 (47.53%), Query Frame = 3
Query: 240 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQD-PERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS--KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 3650
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E L+ S S+L + K I C V+N AT + P ++L ++I TPP P+I K V+++I + C +P G PA SL W W+ ++N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ W YN + E +E PK YS + WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+ + D +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 43 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWR-------WSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFF---SSYN--------------------YFDENFDEIPKKYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 1125
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000056135.1 (SMESG000056135.1) HSP 1 Score: 416.772 bits (1070), Expect = 2.838e-122 Identity = 331/1154 (28.68%), Postives = 535/1154 (46.36%), Query Frame = 3
Query: 240 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPXXXXXXXXXXXXXPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS--KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 3650
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E+ R +L T +S + + I PP P+I K V+++I + C +P G PA SL W W++ +N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ W YN + E +E PK YS + WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+ + D +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 43 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIEKHFMCRYKLCHPT------------ISGISKPSN---------------IKAPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRW-------SNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFF---SSYN--------------------YFDENFDEIPKKYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 1101 The following BLAST results are available for this feature:
BLAST of NPHS1-6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005845 ID=SMED30005845|Name=NPHS1-6|organism=Schmidtea mediterranea sexual|type=transcript|length=5173bpback to top protein sequence of SMED30005845-orf-1 >SMED30005845-orf-1 ID=SMED30005845-orf-1|Name=SMED30005845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1671bp MFAKKWNCFLKFFLVLILIGSFVSSQHPDQSFDDSDLTDSEMSSGAKIKQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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