SMED30005839

Overview
NameSMED30005839
Smed IDSMED30005839
Length (bp)881
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005839 (SMED30005839) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005839 (SMED30005839) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005839 (SMED30005839) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005839 (SMED30005839) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005839

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005839SMESG000005595.1 SmedASXL_068856SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30005839SMESG000005595.1 SmedASXL_068856SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005839 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004664:35659..36635 +4346
v31.005216:37101..38078 +4136
Homology
[Close]
BLAST of SMED30005839 vs. Planmine SMEST
Match: SMESG000005595.1 (SMESG000005595.1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.964e-18
Identity = 38/39 (97.44%), Postives = 39/39 (100.00%), Query Frame = 2 
Query:  245 VPSVYKQTHHNANRRPEKRIREQNSRYSRKDNCAPDLRS 361
            +PSVYKQTHHNANRRPEKRIREQNSRYSRKDNCAPDLRS
Sbjct:  360 LPSVYKQTHHNANRRPEKRIREQNSRYSRKDNCAPDLRS 398
[Close]
BLAST of SMED30005839 vs. Planmine SMEST
Match: SMESG000005595.1 (SMESG000005595.1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.851e-14
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 2 
Query:  392 MTEARKRIHKQIYSSYKRKCHPECRIASHSSIY 490
            MTEARKRIHKQIYSSYKRKCHPECRIASHSSIY
Sbjct:    1 MTEARKRIHKQIYSSYKRKCHPECRIASHSSIY 33
[Close]
BLAST of SMED30005839 vs. Planmine SMEST
Match: SMESG000051975.1 (SMESG000051975.1)

HSP 1 Score: 50.0618 bits (118), Expect = 5.693e-11
Identity = 25/46 (54.35%), Postives = 31/46 (67.39%), Query Frame = 3 
Query:  525 YENERVKNIYQQLDTTRVTLLEFNQLIKAQKAQIRAIETSLSASQE 662
            +E +R+K IY   D  R  L E NQL +AQK QIRA+E +LSAS E
Sbjct:   49 FEQKRLKRIYPVFDANRAALKEANQLKQAQKTQIRALEVALSASHE 94

HSP 2 Score: 35.4242 bits (80), Expect = 5.693e-11
Identity = 22/45 (48.89%), Postives = 28/45 (62.22%), Query Frame = 1 
Query:  685 GIHPAQLAYKEQGTRNVTGE*TNPIRHKQGKAERARFWLLRVEPK 819
            G  PAQL  +E+ T +  G  +N  R K GKAERAR  L+RV+ K
Sbjct:  103 GFVPAQLPDRERATTSDGGP-SNQRRCKPGKAERARLQLMRVKSK 146
[Close]
BLAST of SMED30005839 vs. Planmine SMEST
Match: SMESG000062790.1 (SMESG000062790.1)

HSP 1 Score: 43.1282 bits (100), Expect = 4.461e-7
Identity = 21/47 (44.68%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  522 AYENERVKNIYQQLDTTRVTLLEFNQLIKAQKAQIRAIETSLSASQE 662
             +E +R + IY++LD  R  L E NQ   AQ+  IRA E +L+ASQ+
Sbjct:   23 CFEPKRPQKIYRELDACRTALKEANQTNHAQRTLIRAFELALTASQD 69

HSP 2 Score: 29.261 bits (64), Expect = 4.461e-7
Identity = 23/45 (51.11%), Postives = 26/45 (57.78%), Query Frame = 1 
Query:  688 IHPAQLAYKEQGTRNVTGE*TNPIR-HKQGKAERARFWLLRVEPK 819
              PAQL Y+E+     T E T P R HK GKAE AR  L R +PK
Sbjct:   79 FDPAQLIYRER-----TAE-TFPQRKHKPGKAEPARRRLQRADPK 117
[Close]
BLAST of SMED30005839 vs. Planmine SMEST
Match: SMESG000068632.1 (SMESG000068632.1)

HSP 1 Score: 36.1946 bits (82), Expect = 8.880e-7
Identity = 18/39 (46.15%), Postives = 25/39 (64.10%), Query Frame = 1 
Query:  694 PAQLAYKEQGTRNVTGE*TNPIRHKQGKAERARFWLLRV 810
            PAQLAY++Q +     +   P RH+ GKAERA+   L+V
Sbjct: 1156 PAQLAYRDQMSPATPKD--KPNRHRPGKAERAKLRRLKV 1192

HSP 2 Score: 34.6538 bits (78), Expect = 8.880e-7
Identity = 17/45 (37.78%), Postives = 28/45 (62.22%), Query Frame = 3 
Query:  528 ENERVKNIYQQLDTTRVTLLEFNQLIKAQKAQIRAIETSLSASQE 662
            E ++ K +YQ+++  +       QL +  K QI+ +ET+LSASQE
Sbjct: 1100 EPKKAKAVYQRMEAAQKGWASERQLTQNLKTQIKGLETALSASQE 1144
The following BLAST results are available for this feature:
BLAST of SMED30005839 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005839 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005595.19.964e-1897.44SMESG000005595.1[more]
SMESG000005595.14.851e-14100.00SMESG000005595.1[more]
SMESG000051975.15.693e-1154.35SMESG000051975.1[more]
SMESG000062790.14.461e-744.68SMESG000062790.1[more]
SMESG000068632.18.880e-746.15SMESG000068632.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005839 ID=SMED30005839|Name=SMED30005839|organism=Schmidtea mediterranea sexual|type=transcript|length=881bp
CAACACCGCGACTGGTAACGAAGATAGCAACAACGGTAATAAATGATAAA
GATAGTGATAATGTCAGCACTAATGACCATACGGACAGGCCTAGTCGCGT
TAGTTGAAGAATGTAGTATAGCAATAAAACCAAACATTACTAACCACAAT
GTTAATTAGACAGTGATAATGAAATAGAGATAGAAATAAATGTAAGTTTG
CAACAGTACTGAGAATTCGGCGGTGACTCACTGGTTAAACTGCAGTACCC
TCAGTATATAAACAAACTCATCACAACGCTAATCGAAGACCAGAGAAACG
GATCAGGGAACAAAATTCGCGTTATAGCAGAAAAGACAATTGTGCTCCGG
ATTTAAGGTCGGCGATTCCGAACTGACGTTTCAACAACGGAATGACAGAA
GCAAGAAAACGTATTCATAAGCAGATTTATAGTTCATATAAACGGAAATG
TCACCCGGAGTGCCGAATAGCAAGCCATTCGTCGATTTATTGTGAATTTA
TATGAAATTTATTTACCTAACGCGTATGAAAACGAACGGGTAAAAAATAT
ATACCAACAGCTGGATACAACCCGAGTCACCTTATTGGAGTTTAATCAGC
TCATAAAGGCTCAGAAGGCCCAGATCCGAGCCATAGAAACCTCGTTGTCG
GCAAGTCAAGAGGTAATATTGTAACATATTCCCTGGTATTCACCCAGCTC
AACTGGCTTACAAAGAGCAAGGTACAAGAAATGTGACAGGAGAGTAAACA
AACCCAATACGACACAAACAAGGAAAGGCCGAGCGAGCTCGATTTTGGTT
ATTACGGGTCGAACCGAAAGCGGGGAATTAAATTTAGTATAGATGAAAAT
TATTGTTATTTCATTTTGCAATAAAATTTTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.556