Alpha-1,4 glucan phosphorylase

Overview
NameAlpha-1,4 glucan phosphorylase
Smed IDSMED30005829
Length (bp)678
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005829

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30005829h1SMcG0002712 Contig16039uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30005829h1SMcG0002712 Contig16039newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
testisSMED30005829h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30005829h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30005829h1SMcG0002712 PL06011A1C11_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGM (glycogen phosphorylase, muscle associated [Source:HGNC Symbol;Acc:HGNC:9726])

HSP 1 Score: 286.574 bits (732), Expect = 7.025e-92
Identity = 133/215 (61.86%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  F  ++  +  + K+K ENK+KFA Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LHVI LYNR+K       +PRTVMIGGKAAPGYH+AK+ I+L++ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  381 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 595          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGM (glycogen phosphorylase, muscle associated [Source:HGNC Symbol;Acc:HGNC:9726])

HSP 1 Score: 286.574 bits (732), Expect = 4.579e-91
Identity = 133/215 (61.86%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  F  ++  +  + K+K ENK+KFA Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LHVI LYNR+K       +PRTVMIGGKAAPGYH+AK+ I+L++ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGB (glycogen phosphorylase B [Source:HGNC Symbol;Acc:HGNC:9723])

HSP 1 Score: 285.034 bits (728), Expect = 1.831e-90
Identity = 132/215 (61.40%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P KFQNKTNGITPRRWLLLCNP L+D ++  IG+ ++T+LS L +L     ++  +  + K+K ENK+KF+ +++K+YK+ I+P+S+FD  VKRIHEYKRQ+LN LHV+ LYNR+K +     +PRTVMIGGKAAPGYH+AKL IKL++ +G ++N+DP++ ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGL (glycogen phosphorylase L [Source:HGNC Symbol;Acc:HGNC:9725])

HSP 1 Score: 280.411 bits (716), Expect = 4.969e-89
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDF EL P+KFQNKTNGITPRRWLLLCNP L++ +   IG+ ++ +LS L++L  F G+   + ++ K+K ENK+KF+ +++ +YK+ I+P+S+FD QVKRIHEYKRQ+LN LHVI +YNR+K + K   +PRTV+IGGKAAPGYH+AK+ IKLI+ V  ++NNDP++ +KL+++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Match: PYGL (glycogen phosphorylase L [Source:HGNC Symbol;Acc:HGNC:9725])

HSP 1 Score: 280.411 bits (716), Expect = 5.685e-89
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDF EL P+KFQNKTNGITPRRWLLLCNP L++ +   IG+ ++ +LS L++L  F G+   + ++ K+K ENK+KF+ +++ +YK+ I+P+S+FD QVKRIHEYKRQ+LN LHVI +YNR+K + K   +PRTV+IGGKAAPGYH+AK+ IKLI+ V  ++NNDP++ +KL+++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  435 FKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 649          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 281.182 bits (718), Expect = 1.654e-91
Identity = 131/215 (60.93%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DFYE +P++FQNKTNGITPRRWLLL NP+L+D ++  IG+SWITNL +L +L+E+  +   +  I ++K ENK + A Y+  +Y +N++  SLFD  VKRIHEYKRQ+LN+LHVI LYNR+K N   DM+ RTV+ GGKAAPGYH+AK  I+LI+ V + +NND I+ ++L+++FLENYRV++AEKI PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:  248 FRDFYEFYPDRFQNKTNGITPRRWLLLSNPSLADLIVEKIGESWITNLDELQKLKEYANDAGFLDSIRRVKLENKQQVAQYLSDEYNVNVNAASLFDVHVKRIHEYKRQLLNILHVIALYNRIKENPNIDMVKRTVLYGGKAAPGYHMAKQIIRLITAVAEQVNNDAIVGDRLKVIFLENYRVSMAEKIIPASDLSEQISTAGTEASGTGNMKFM 462          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 280.411 bits (716), Expect = 3.995e-89
Identity = 131/215 (60.93%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DFYE +P++FQNKTNGITPRRWLLL NP+L+D ++  IG+SWITNL +L +L+E+  +   +  I ++K ENK + A Y+  +Y +N++  SLFD  VKRIHEYKRQ+LN+LHVI LYNR+K N   DM+ RTV+ GGKAAPGYH+AK  I+LI+ V + +NND I+ ++L+++FLENYRV++AEKI PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:  470 FRDFYEFYPDRFQNKTNGITPRRWLLLSNPSLADLIVEKIGESWITNLDELQKLKEYANDAGFLDSIRRVKLENKQQVAQYLSDEYNVNVNAASLFDVHVKRIHEYKRQLLNILHVIALYNRIKENPNIDMVKRTVLYGGKAAPGYHMAKQIIRLITAVAEQVNNDAIVGDRLKVIFLENYRVSMAEKIIPASDLSEQISTAGTEASGTGNMKFM 684          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 280.796 bits (717), Expect = 5.237e-89
Identity = 131/215 (60.93%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DFYE +P++FQNKTNGITPRRWLLL NP+L+D ++  IG+SWITNL +L +L+E+  +   +  I ++K ENK + A Y+  +Y +N++  SLFD  VKRIHEYKRQ+LN+LHVI LYNR+K N   DM+ RTV+ GGKAAPGYH+AK  I+LI+ V + +NND I+ ++L+++FLENYRV++AEKI PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:  506 FRDFYEFYPDRFQNKTNGITPRRWLLLSNPSLADLIVEKIGESWITNLDELQKLKEYANDAGFLDSIRRVKLENKQQVAQYLSDEYNVNVNAASLFDVHVKRIHEYKRQLLNILHVIALYNRIKENPNIDMVKRTVLYGGKAAPGYHMAKQIIRLITAVAEQVNNDAIVGDRLKVIFLENYRVSMAEKIIPASDLSEQISTAGTEASGTGNMKFM 720          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Match: pygl-1 (Alpha-1,4 glucan phosphorylase [Source:UniProtKB/TrEMBL;Acc:Q9N5U1])

HSP 1 Score: 115.161 bits (287), Expect = 3.307e-31
Identity = 58/85 (68.24%), Postives = 75/85 (88.24%), Query Frame = 1
Query:  424 MIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            M+ RTV+ GGKAAPGYH+AK  I+LI+ V + +NND I+ ++L+++FLENYRV++AEKI PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:    1 MVKRTVLYGGKAAPGYHMAKQIIRLITAVAEQVNNDAIVGDRLKVIFLENYRVSMAEKIIPASDLSEQISTAGTEASGTGNMKFM 85          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Match: GlyP (gene:FBgn0004507 transcript:FBtr0100485)

HSP 1 Score: 269.626 bits (688), Expect = 4.732e-85
Identity = 132/214 (61.68%), Postives = 168/214 (78.50%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKF 675
            F DFYE+ P KFQNKTNGITPRRWLLLCNP LSD +   IGD W  +L  L  L+++  + +    + ++K ENK+K A  ++K Y + I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +   +  PRT+MIGGKAAPGY++AK  IKLI  VG ++NNDPI+ +KL+++FLENYRVTLAEKI PA++LS+QISTAGTEASGTGNMKF
Sbjct:  469 FHDFYEMEPQKFQNKTNGITPRRWLLLCNPGLSDLIAEKIGDEWPVHLDQLVALKKWAKDPNFQRNVARVKQENKLKLAAILEKDYGVKINPSSMFDIQVKRIHEYKRQLLNCLHIITLYNRIKKDPTANFTPRTIMIGGKAAPGYYVAKQIIKLICAVGNVVNNDPIVGDKLKVIFLENYRVTLAEKIMPAADLSEQISTAGTEASGTGNMKF 682          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Match: GlyP (gene:FBgn0004507 transcript:FBtr0077828)

HSP 1 Score: 269.626 bits (688), Expect = 4.732e-85
Identity = 132/214 (61.68%), Postives = 168/214 (78.50%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKF 675
            F DFYE+ P KFQNKTNGITPRRWLLLCNP LSD +   IGD W  +L  L  L+++  + +    + ++K ENK+K A  ++K Y + I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +   +  PRT+MIGGKAAPGY++AK  IKLI  VG ++NNDPI+ +KL+++FLENYRVTLAEKI PA++LS+QISTAGTEASGTGNMKF
Sbjct:  469 FHDFYEMEPQKFQNKTNGITPRRWLLLCNPGLSDLIAEKIGDEWPVHLDQLVALKKWAKDPNFQRNVARVKQENKLKLAAILEKDYGVKINPSSMFDIQVKRIHEYKRQLLNCLHIITLYNRIKKDPTANFTPRTIMIGGKAAPGYYVAKQIIKLICAVGNVVNNDPIVGDKLKVIFLENYRVTLAEKIMPAADLSEQISTAGTEASGTGNMKF 682          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygmb (phosphorylase, glycogen, muscle b [Source:ZFIN;Acc:ZDB-GENE-040426-1206])

HSP 1 Score: 279.641 bits (714), Expect = 1.168e-88
Identity = 131/215 (60.93%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY++ P KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +LS L +L +F  +  ++  I K+K ENKMKFA ++++QYK+ I+PNS+FD  VKRIHEYKRQ+LN LH+I LYNR+K        PRT+MIGGKAAPGYH AK+ IKLI+ +G ++NND ++ ++L+++FLENYRVTLAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYDVDPQKFQNKTNGITPRRWLVMCNPGLAEVIAERIGEDYIRDLSQLKKLLKFVDDDALIRDIAKVKQENKMKFAVHLEEQYKVKINPNSMFDVHVKRIHEYKRQLLNCLHIITLYNRIKKEPNKAWTPRTIMIGGKAAPGYHTAKMIIKLITSIGDVVNNDIVVGDRLKVIFLENYRVTLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygmb (phosphorylase, glycogen, muscle b [Source:ZFIN;Acc:ZDB-GENE-040426-1206])

HSP 1 Score: 279.641 bits (714), Expect = 1.168e-88
Identity = 131/215 (60.93%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY++ P KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +LS L +L +F  +  ++  I K+K ENKMKFA ++++QYK+ I+PNS+FD  VKRIHEYKRQ+LN LH+I LYNR+K        PRT+MIGGKAAPGYH AK+ IKLI+ +G ++NND ++ ++L+++FLENYRVTLAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYDVDPQKFQNKTNGITPRRWLVMCNPGLAEVIAERIGEDYIRDLSQLKKLLKFVDDDALIRDIAKVKQENKMKFAVHLEEQYKVKINPNSMFDVHVKRIHEYKRQLLNCLHIITLYNRIKKEPNKAWTPRTIMIGGKAAPGYHTAKMIIKLITSIGDVVNNDIVVGDRLKVIFLENYRVTLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygl (phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZDB-GENE-041205-1])

HSP 1 Score: 279.641 bits (714), Expect = 1.402e-88
Identity = 133/215 (61.86%), Postives = 175/215 (81.40%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DF EL P KFQNKTNGITPRRWLLLCNP L+D +   IG+ ++ +L+ L +L +   ++  +  + K+K +NK+KFAHY++K YK++I+P S+FD  VKRIHEYKRQ+LN LHVI +YNR+KS+     IPRTV+IGGKAAPGYH+AK+ IKLI+ V  ++NNDP+I  KL+++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  468 FQDFSELEPEKFQNKTNGITPRRWLLLCNPGLADLIAEAIGEEYVKDLTQLQKLNDLVDDEAFIRDVAKVKQDNKLKFAHYLEKIYKVDINPASMFDVHVKRIHEYKRQLLNCLHVITMYNRIKSHPTKPFIPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPVIGRKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 682          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygl (phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZDB-GENE-041205-1])

HSP 1 Score: 279.641 bits (714), Expect = 2.238e-88
Identity = 133/215 (61.86%), Postives = 175/215 (81.40%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DF EL P KFQNKTNGITPRRWLLLCNP L+D +   IG+ ++ +L+ L +L +   ++  +  + K+K +NK+KFAHY++K YK++I+P S+FD  VKRIHEYKRQ+LN LHVI +YNR+KS+     IPRTV+IGGKAAPGYH+AK+ IKLI+ V  ++NNDP+I  KL+++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  494 FQDFSELEPEKFQNKTNGITPRRWLLLCNPGLADLIAEAIGEEYVKDLTQLQKLNDLVDDEAFIRDVAKVKQDNKLKFAHYLEKIYKVDINPASMFDVHVKRIHEYKRQLLNCLHVITMYNRIKSHPTKPFIPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPVIGRKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 708          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Match: pygma (phosphorylase, glycogen, muscle A [Source:ZFIN;Acc:ZDB-GENE-050522-482])

HSP 1 Score: 276.174 bits (705), Expect = 2.328e-87
Identity = 129/215 (60.00%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P+KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L +F  ++  +  I K+K ENK+KFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH AK+ I+LI+ +G+++NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYEMDPHKFQNKTNGITPRRWLVMCNPGLAEVIAEKIGEDFIRDLDQLQKLRDFVNDEAFIRDIAKVKQENKLKFAVHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKQWTPRTIMIGGKAAPGYHTAKMIIRLITAIGEVVNNDPVVGDRLKVIFLENYRVTLAEKAIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: taf8 (TATA-box binding protein associated factor 8 [Source:Xenbase;Acc:XB-GENE-491356])

HSP 1 Score: 292.738 bits (748), Expect = 2.950e-94
Identity = 139/215 (64.65%), Postives = 181/215 (84.19%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY+L PNKFQNKTNGITPRRWL+LCNP LSD +   IG+ ++T+LS L +L +F  ++  VH + K+K ENK+KFA Y++++YK+ I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +     +PRTVMIGGKAAPGYH+AK+ IKLI+ +  I+NNDP+I ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  378 FKDFYDLEPNKFQNKTNGITPRRWLMLCNPGLSDIIAEKIGEDFVTDLSQLRKLLDFVNDESFVHDVAKVKQENKLKFAAYLEQEYKVKINPSSVFDVQVKRIHEYKRQLLNCLHIITLYNRIKKDPSKIFVPRTVMIGGKAAPGYHMAKMIIKLINSIASIVNNDPVIGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 592          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: taf8 (TATA-box binding protein associated factor 8 [Source:Xenbase;Acc:XB-GENE-491356])

HSP 1 Score: 292.352 bits (747), Expect = 1.557e-93
Identity = 139/215 (64.65%), Postives = 181/215 (84.19%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY+L PNKFQNKTNGITPRRWL+LCNP LSD +   IG+ ++T+LS L +L +F  ++  VH + K+K ENK+KFA Y++++YK+ I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +     +PRTVMIGGKAAPGYH+AK+ IKLI+ +  I+NNDP+I ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  448 FKDFYDLEPNKFQNKTNGITPRRWLMLCNPGLSDIIAEKIGEDFVTDLSQLRKLLDFVNDESFVHDVAKVKQENKLKFAAYLEQEYKVKINPSSVFDVQVKRIHEYKRQLLNCLHIITLYNRIKKDPSKIFVPRTVMIGGKAAPGYHMAKMIIKLINSIASIVNNDPVIGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 662          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: taf8 (TATA-box binding protein associated factor 8 [Source:Xenbase;Acc:XB-GENE-491356])

HSP 1 Score: 292.352 bits (747), Expect = 2.563e-93
Identity = 139/215 (64.65%), Postives = 181/215 (84.19%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY+L PNKFQNKTNGITPRRWL+LCNP LSD +   IG+ ++T+LS L +L +F  ++  VH + K+K ENK+KFA Y++++YK+ I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +     +PRTVMIGGKAAPGYH+AK+ IKLI+ +  I+NNDP+I ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYDLEPNKFQNKTNGITPRRWLMLCNPGLSDIIAEKIGEDFVTDLSQLRKLLDFVNDESFVHDVAKVKQENKLKFAAYLEQEYKVKINPSSVFDVQVKRIHEYKRQLLNCLHIITLYNRIKKDPSKIFVPRTVMIGGKAAPGYHMAKMIIKLINSIASIVNNDPVIGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: taf8 (TATA-box binding protein associated factor 8 [Source:Xenbase;Acc:XB-GENE-491356])

HSP 1 Score: 291.967 bits (746), Expect = 2.602e-93
Identity = 139/215 (64.65%), Postives = 181/215 (84.19%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY+L PNKFQNKTNGITPRRWL+LCNP LSD +   IG+ ++T+LS L +L +F  ++  VH + K+K ENK+KFA Y++++YK+ I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +     +PRTVMIGGKAAPGYH+AK+ IKLI+ +  I+NNDP+I ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  462 FKDFYDLEPNKFQNKTNGITPRRWLMLCNPGLSDIIAEKIGEDFVTDLSQLRKLLDFVNDESFVHDVAKVKQENKLKFAAYLEQEYKVKINPSSVFDVQVKRIHEYKRQLLNCLHIITLYNRIKKDPSKIFVPRTVMIGGKAAPGYHMAKMIIKLINSIASIVNNDPVIGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 676          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Match: taf8 (TATA-box binding protein associated factor 8 [Source:Xenbase;Acc:XB-GENE-491356])

HSP 1 Score: 292.352 bits (747), Expect = 2.618e-93
Identity = 139/215 (64.65%), Postives = 181/215 (84.19%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY+L PNKFQNKTNGITPRRWL+LCNP LSD +   IG+ ++T+LS L +L +F  ++  VH + K+K ENK+KFA Y++++YK+ I+P+S+FD QVKRIHEYKRQ+LN LH+I LYNR+K +     +PRTVMIGGKAAPGYH+AK+ IKLI+ +  I+NNDP+I ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYDLEPNKFQNKTNGITPRRWLMLCNPGLSDIIAEKIGEDFVTDLSQLRKLLDFVNDESFVHDVAKVKQENKLKFAAYLEQEYKVKINPSSVFDVQVKRIHEYKRQLLNCLHIITLYNRIKKDPSKIFVPRTVMIGGKAAPGYHMAKMIIKLINSIASIVNNDPVIGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 286.574 bits (732), Expect = 5.536e-92
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KF+ Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LH+I LYNR+K      M+PRT+MIGGKAAPGYH+AK+ IKLI+ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  381 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 595          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 286.574 bits (732), Expect = 5.536e-92
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KF+ Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LH+I LYNR+K      M+PRT+MIGGKAAPGYH+AK+ IKLI+ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  381 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 595          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 286.574 bits (732), Expect = 2.713e-91
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KF+ Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LH+I LYNR+K      M+PRT+MIGGKAAPGYH+AK+ IKLI+ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygm (muscle glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97830])

HSP 1 Score: 286.574 bits (732), Expect = 2.713e-91
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KF+ Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LH+I LYNR+K      M+PRT+MIGGKAAPGYH+AK+ IKLI+ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Match: Pygl (liver glycogen phosphorylase [Source:MGI Symbol;Acc:MGI:97829])

HSP 1 Score: 281.182 bits (718), Expect = 7.082e-90
Identity = 134/219 (61.19%), Postives = 181/219 (82.65%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF EL P+KFQNKTNGITPRRWLLLCNP L+D +   IG+ ++ +LS L++L  F  +   + +I K+K ENK+KF+ +++K+YK+ I+P+S+FD  VKRIHEYKRQ+LN LHVI +YNR+K + K   +PRTV+IGGKAAPGYH+AK+ IKLI+ V +++NNDP++ +KL+++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  374 KTQVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLADLIAEKIGEDYVKDLSQLTKLHSFVSDDIFLREIAKVKQENKLKFSQFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVITMYNRIKKDPKKFFVPRTVIIGGKAAPGYHMAKMIIKLITSVAEVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 592          
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P00489|PYGM_RABIT (Glycogen phosphorylase, muscle form OS=Oryctolagus cuniculus OX=9986 GN=PYGM PE=1 SV=3)

HSP 1 Score: 286.574 bits (732), Expect = 1.757e-90
Identity = 134/215 (62.33%), Postives = 180/215 (83.72%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KFA Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LHVI LYNR+K      ++PRTVMIGGKAAPGYH+AK+ IKLI+ +G ++N+DP++ ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEIIAERIGEEYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFVVPRTVMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|Q9WUB3|PYGM_MOUSE (Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3)

HSP 1 Score: 286.574 bits (732), Expect = 1.898e-90
Identity = 132/215 (61.40%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  +  ++  +  + K+K ENK+KF+ Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LH+I LYNR+K      M+PRT+MIGGKAAPGYH+AK+ IKLI+ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P11217|PYGM_HUMAN (Glycogen phosphorylase, muscle form OS=Homo sapiens OX=9606 GN=PYGM PE=1 SV=6)

HSP 1 Score: 286.574 bits (732), Expect = 2.199e-90
Identity = 133/215 (61.86%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL+LCNP L++ +   IG+ +I++L  L +L  F  ++  +  + K+K ENK+KFA Y++++YK++I+PNSLFD QVKRIHEYKRQ+LN LHVI LYNR+K       +PRTVMIGGKAAPGYH+AK+ I+L++ +G ++N+DP + ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P79334|PYGM_BOVIN (Glycogen phosphorylase, muscle form OS=Bos taurus OX=9913 GN=PYGM PE=1 SV=3)

HSP 1 Score: 285.419 bits (729), Expect = 5.495e-90
Identity = 133/215 (61.86%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P+KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L  +  ++  +  + K+K ENK+KF+ Y++K+YK++I+PNSLFD QVKRIHEYKRQ+LN LHVI LYNR+K       +PRTVMIGGKAAPGYH+AK+ IKLI+ +G ++N+DP++ ++LR++FLENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPHKFQNKTNGITPRRWLVMCNPGLAEIIAERIGEEYIADLDQLRKLLSYVDDESFIRDVAKVKQENKLKFSAYLEKEYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFFVPRTVMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Match: sp|P11216|PYGB_HUMAN (Glycogen phosphorylase, brain form OS=Homo sapiens OX=9606 GN=PYGB PE=1 SV=5)

HSP 1 Score: 285.034 bits (728), Expect = 8.792e-90
Identity = 132/215 (61.40%), Postives = 178/215 (82.79%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P KFQNKTNGITPRRWLLLCNP L+D ++  IG+ ++T+LS L +L     ++  +  + K+K ENK+KF+ +++K+YK+ I+P+S+FD  VKRIHEYKRQ+LN LHV+ LYNR+K +     +PRTVMIGGKAAPGYH+AKL IKL++ +G ++N+DP++ ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A183KLL0 (Alpha-1,4 glucan phosphorylase OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15924 PE=3 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 3.082e-100
Identity = 146/221 (66.06%), Postives = 187/221 (84.62%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLI--GDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF ELWPNKFQNKTNGITPRRWLLLCNPNLSD ++  +   +SWI NL+++++L+    + + + Q+++IK ENK KFA Y+++ Y + I+P SLFD QVKRIHEYKRQ+LN LHVI LYNR+K+N +  + PRTVMIGGKAAPGYH+AKL IKLI+ VGK++NNDP+++ +++L+FLENYRV+LAEKIFPA+ELS+QISTAGTEASGTGNMKFM
Sbjct:   37 KTQTFKDFAELWPNKFQNKTNGITPRRWLLLCNPNLSDLIMEGMNGSESWIVNLNEIAQLKSRVNDVNFLRQLIRIKRENKAKFASYLEQHYGVTINPASLFDIQVKRIHEYKRQLLNCLHVITLYNRIKANPEIPICPRTVMIGGKAAPGYHMAKLIIKLINSVGKVVNNDPVVRGRIKLIFLENYRVSLAEKIFPAAELSEQISTAGTEASGTGNMKFM 257          
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A3P7YSD3 (Alpha-1,4 glucan phosphorylase OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS10672 PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 4.144e-98
Identity = 146/221 (66.06%), Postives = 187/221 (84.62%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLI--GDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF ELWPNKFQNKTNGITPRRWLLLCNPNLSD ++  +   +SWI NL+++++L+    + + + Q+++IK ENK KFA Y+++ Y + I+P SLFD QVKRIHEYKRQ+LN LHVI LYNR+K+N +  + PRTVMIGGKAAPGYH+AKL IKLI+ VGK++NNDP+++ +++L+FLENYRV+LAEKIFPA+ELS+QISTAGTEASGTGNMKFM
Sbjct:  128 KTQTFKDFAELWPNKFQNKTNGITPRRWLLLCNPNLSDLIMEGMNGSESWIVNLNEIAQLKSRVNDVNFLRQLIRIKRENKAKFASYLEQHYGVTINPASLFDIQVKRIHEYKRQLLNCLHVITLYNRIKANPEIPICPRTVMIGGKAAPGYHMAKLIIKLINSVGKVVNNDPVVRGRIKLIFLENYRVSLAEKIFPAAELSEQISTAGTEASGTGNMKFM 348          
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A183M3P7 (Alpha-1,4 glucan phosphorylase OS=Schistosoma margrebowiei OX=48269 PE=3 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 4.572e-98
Identity = 146/221 (66.06%), Postives = 187/221 (84.62%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLI--GDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF ELWPNKFQNKTNGITPRRWLLLCNPNLSD ++  +   +SWI NL+++++L+    + + + Q+++IK ENK KFA Y+++ Y + I+P SLFD QVKRIHEYKRQ+LN LHVI LYNR+K+N +  + PRTVMIGGKAAPGYH+AKL IKLI+ VGK++NNDP+++ +++L+FLENYRV+LAEKIFPA+ELS+QISTAGTEASGTGNMKFM
Sbjct:  128 KTQTFKDFAELWPNKFQNKTNGITPRRWLLLCNPNLSDLIMEGMNGSESWIVNLNEIAQLKSRVNDVNFLRQLIRIKRENKAKFASYLEQHYGVTINPASLFDIQVKRIHEYKRQLLNCLHVITLYNRIKANPEIPICPRTVMIGGKAAPGYHMAKLIIKLINSVGKVVNNDPVVRGRIKLIFLENYRVSLAEKIFPAAELSEQISTAGTEASGTGNMKFM 348          
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: A0A093IXG6 (Alpha-1,4 glucan phosphorylase (Fragment) OS=Eurypyga helias OX=54383 GN=N326_05707 PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 5.228e-97
Identity = 137/215 (63.72%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYEL P KFQNKTNGITPRRWLLLCNP L+D +   IG+ +IT+LS L +L +F   +  +  + K+K ENK+KFA Y+++QYK+ I+P+S+FD QVKRIHEYKRQ+LN LH + LYNR++S+     +PRT+MIGGKAAPGYH+AK+ IKLI+ +G++INNDP + ++L+++FLENYRV+LAEK+ PA++LSQQISTAGTEASGTGNMKFM
Sbjct:    1 FKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADVIAEKIGEGFITDLSQLKKLLDFVNNEPFIRDVAKVKQENKLKFAAYLEEQYKVKINPSSMFDVQVKRIHEYKRQLLNCLHAVTLYNRIRSDPSKSFVPRTIMIGGKAAPGYHMAKMIIKLITSIGEVINNDPYVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFM 215          
BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Match: Q5BY06 (Alpha-1,4 glucan phosphorylase (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=2)

HSP 1 Score: 298.13 bits (762), Expect = 1.110e-96
Identity = 147/221 (66.52%), Postives = 186/221 (84.16%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIG--DSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF ELWP+KFQNKTNGITPRRWLLLCNPNLSD ++  +   DSWI NL ++++L+    + + + Q+++IK ENK KFA Y+++ Y + I+P SLFD QVKRIHEYKRQ+LN LHVI LYNR+K+N +  + PRTVMIGGKAAPGYH+AKL IKLI+ VGKI+NNDP+++ +++L+FLENYRV+LAEKIFPA+ELS+QISTAGTEASGTGNMKFM
Sbjct:   56 KTQTFKDFAELWPDKFQNKTNGITPRRWLLLCNPNLSDLIMEGMNGDDSWIVNLHEIAQLKSRVNDVNFLRQLMRIKRENKAKFASYLEQHYGVTINPASLFDIQVKRIHEYKRQLLNCLHVITLYNRIKANPELPVCPRTVMIGGKAAPGYHMAKLIIKLINSVGKIVNNDPVVRGRIKLIFLENYRVSLAEKIFPAAELSEQISTAGTEASGTGNMKFM 276          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygmb (glycogen phosphorylase, muscle form-like [Source:NCBI gene;Acc:103042589])

HSP 1 Score: 275.018 bits (702), Expect = 1.061e-87
Identity = 131/215 (60.93%), Postives = 173/215 (80.47%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L +F  +   +  + KIK ENKMKFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH+AK+ I+LI+ +G+I+NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  374 FKDFYEVDPQKFQNKTNGITPRRWLVMCNPGLAEVIADRIGEDYIRDLGQLKKLLKFVDDDAFIRDVAKIKQENKMKFAVHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKAWTPRTIMIGGKAAPGYHMAKMIIRLITAIGEIVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFM 588          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygmb (glycogen phosphorylase, muscle form-like [Source:NCBI gene;Acc:103042589])

HSP 1 Score: 275.018 bits (702), Expect = 5.459e-87
Identity = 131/215 (60.93%), Postives = 173/215 (80.47%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L +F  +   +  + KIK ENKMKFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH+AK+ I+LI+ +G+I+NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYEVDPQKFQNKTNGITPRRWLVMCNPGLAEVIADRIGEDYIRDLGQLKKLLKFVDDDAFIRDVAKIKQENKMKFAVHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKAWTPRTIMIGGKAAPGYHMAKMIIRLITAIGEIVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygmb (glycogen phosphorylase, muscle form-like [Source:NCBI gene;Acc:103042589])

HSP 1 Score: 275.018 bits (702), Expect = 5.459e-87
Identity = 131/215 (60.93%), Postives = 173/215 (80.47%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L +F  +   +  + KIK ENKMKFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH+AK+ I+LI+ +G+I+NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYEVDPQKFQNKTNGITPRRWLVMCNPGLAEVIADRIGEDYIRDLGQLKKLLKFVDDDAFIRDVAKIKQENKMKFAVHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKAWTPRTIMIGGKAAPGYHMAKMIIRLITAIGEIVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygma (phosphorylase, glycogen, muscle A [Source:ZFIN;Acc:ZDB-GENE-050522-482])

HSP 1 Score: 273.478 bits (698), Expect = 2.077e-86
Identity = 128/215 (59.53%), Postives = 172/215 (80.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P+KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L  F  +   +  + K+K ENK+KFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH AK+ I+LI+ +G+++NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  468 FKDFYEMDPHKFQNKTNGITPRRWLVMCNPGLAEVIAEKIGEDFIRDLDQLKKLLNFVNDDAFIRDVAKVKQENKLKFATHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKQWTPRTIMIGGKAAPGYHTAKMIIRLITAIGEVVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFM 682          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Match: pygma (phosphorylase, glycogen, muscle A [Source:ZFIN;Acc:ZDB-GENE-050522-482])

HSP 1 Score: 273.092 bits (697), Expect = 5.061e-86
Identity = 128/215 (59.53%), Postives = 172/215 (80.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P+KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L +L  F  +   +  + K+K ENK+KFA ++++ YK+ I+PNS+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRT+MIGGKAAPGYH AK+ I+LI+ +G+++NNDP++ ++L+++FLENYRVTLAEK  PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  517 FKDFYEMDPHKFQNKTNGITPRRWLVMCNPGLAEVIAEKIGEDFIRDLDQLKKLLNFVNDDAFIRDVAKVKQENKLKFATHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKQWTPRTIMIGGKAAPGYHTAKMIIRLITAIGEVVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFM 731          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006065.1 (pep scaffold:Pmarinus_7.0:GL476342:314148:364539:-1 gene:ENSPMAG00000005334.1 transcript:ENSPMAT00000006065.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 278.87 bits (712), Expect = 4.115e-89
Identity = 133/215 (61.86%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DFYE+ P KFQNKTNGITPRRWLLLCNP L+D +   IGD +I +L+ L +L++F  +  ++  I K+K ENK+KFA Y +K++ + ++P S+ D QVKRIHEYKRQ+LN LHVI LYNR+K + K    PRTVMIGGKAAPGYH+AK+ I+LI+ VG ++N+DP + ++L+ +FLENYRV+LAEK+ PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:  470 FRDFYEIEPEKFQNKTNGITPRRWLLLCNPGLADVIAERIGDDFIRDLNLLHKLKDFANDDALIRDIAKVKQENKIKFAAYYEKEHGLKLNPASMMDIQVKRIHEYKRQLLNCLHVITLYNRIKKDPKKHYAPRTVMIGGKAAPGYHMAKMIIRLITAVGDVVNHDPKVGDRLKFIFLENYRVSLAEKVIPASDLSEQISTAGTEASGTGNMKFM 684          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008899.1 (pep scaffold:Pmarinus_7.0:GL479616:11553:30183:-1 gene:ENSPMAG00000007996.1 transcript:ENSPMAT00000008899.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 273.092 bits (697), Expect = 1.609e-87
Identity = 127/215 (59.07%), Postives = 173/215 (80.47%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DF+E+ P KFQNKTNGITPRRWLLLCNP L+D +   IGD ++  LS LS+L +   +   +  + K+K ENK+KF+  ++KQY + ++P+S+FD  VKRIHEYKRQ+LN LH+I LYNR+K N     + RT++IGGKAAPGYH+AK+ IKL++ +G ++NNDPI+ ++L+++FLENY+V+LAEK+ PAS+LS+QISTAGTEASGTGNMKFM
Sbjct:  388 FRDFWEIDPKKFQNKTNGITPRRWLLLCNPGLADIIAERIGDEYVNELSQLSKLGDLVDDDAFIRDVAKVKQENKLKFSALLEKQYGVAVNPDSMFDVHVKRIHEYKRQLLNCLHIITLYNRIKRNPTKRFVARTILIGGKAAPGYHMAKMIIKLVTSIGDVVNNDPIVGDRLKVIFLENYKVSLAEKVIPASDLSEQISTAGTEASGTGNMKFM 602          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Yeast
Match: GPH1 (Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; phosphorylation by Cdc28p may coordinately regulate carbohydrate metabolism and the cell cycle; expression is regulated by stress-response elements and by the HOG MAP kinase pathway [Source:SGD;Acc:S000006364])

HSP 1 Score: 167.162 bits (422), Expect = 4.791e-48
Identity = 107/238 (44.96%), Postives = 157/238 (65.97%), Query Frame = 1
Query:   22 QTFKFKDFYELW-PNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGD---SWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYK----INID--PNSLFDFQVKRIHEYKRQMLNVLHVIVLY----NRLKSNSKYDMI----PRTVMI-GGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  FKDF + + P+KF N TNGITPRRWL   NP+L+  +   + D    ++ +++ L++L ++  +K+ + +  ++K  NK++    +KK+      IN +   ++LFD QVKRIHEYKRQ LNV  +I  Y    N LK+ +  + +    PR V I GGK+APGY++AKL IKLI+ V  I+NND  I++ L++VF+ +Y V+ AE I PAS+LS+ ISTAGTEASGT NMKF+
Sbjct:  516 KTTIFKDFVKFYGPSKFVNVTNGITPRRWLKQANPSLAKLISETLNDPTEEYLLDMAKLTQLGKYVEDKEFLKKWNQVKLNNKIRLVDLIKKENDGVDIINREYLDDTLFDMQVKRIHEYKRQQLNVFGIIYRYLAMKNMLKNGASIEEVAKKYPRKVSIFGGKSAPGYYMAKLIIKLINCVADIVNNDESIEHLLKVVFVADYNVSKAEIIIPASDLSEHISTAGTEASGTSNMKFV 753          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Match: EDO33811 (Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7SR65])

HSP 1 Score: 201.445 bits (511), Expect = 3.022e-60
Identity = 113/216 (52.31%), Postives = 146/216 (67.59%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLY-NRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P KFQNKTNG+TPRRWLLLCNP LSD +   I +SWITNLSDL++L++F  +K+ +H  ++                                     K+Q+    + I+ +   LK+N     +PRTVM+GGKAAPGYH+AKL IKLI+ V K++NNDPII +KL++VFLENYRV+LAEK+ PA++LS+QIS AGTEASGTGNMKFM
Sbjct:  455 FKDFYEMSPEKFQNKTNGVTPRRWLLLCNPGLSDLICEKIEESWITNLSDLTKLKKFAKDKNFLHAFMR------------------------------------SKQQLATSNNAILFWVPWLKANPNMTFVPRTVMVGGKAAPGYHMAKLIIKLINSVAKVVNNDPIIGDKLKVVFLENYRVSLAEKVIPAADLSEQISLAGTEASGTGNMKFM 634          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Match: EDO27771 (Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7T8B9])

HSP 1 Score: 49.2914 bits (116), Expect = 1.174e-7
Identity = 21/27 (77.78%), Postives = 25/27 (92.59%), Query Frame = 1
Query:  598 IFPASELSQQISTAGTEASGTGNMKFM 678
            + PA++LS+QIS AGTEASGTGNMKFM
Sbjct:    1 VIPAADLSEQISLAGTEASGTGNMKFM 27          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygmb (glycogen phosphorylase, muscle associated [Source:NCBI gene;Acc:101171113])

HSP 1 Score: 274.633 bits (701), Expect = 6.218e-87
Identity = 128/215 (59.53%), Postives = 174/215 (80.93%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P+KFQNKTNGITPRRWL++CNP L+D +   IG+ +I +L  L +L +F  ++ ++  + K+K ENK+KFA ++++ YK+ I+ +S+FD  VKRIHEYKRQ+LN LH+I LYNR+K        PRT+MIGGKAAPGYH AK+ IKLI+ +G ++NNDP++ ++L+++FLENYRVTLAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYEMDPHKFQNKTNGITPRRWLVMCNPGLADVIAERIGEDYIRDLDQLKKLLDFVDDEALIRDVAKVKQENKLKFAAHLEEHYKVKINSDSMFDIHVKRIHEYKRQLLNCLHIITLYNRIKKEPNKKWTPRTIMIGGKAAPGYHTAKMIIKLITSIGDVVNNDPVVGDRLKVIFLENYRVTLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygb (glycogen phosphorylase B [Source:NCBI gene;Acc:101170949])

HSP 1 Score: 274.633 bits (701), Expect = 8.873e-87
Identity = 129/215 (60.00%), Postives = 175/215 (81.40%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFY++ P+KFQNKTNGITPRRWLLLCNP L+D +   IG+ ++T+L  L  + +F  ++  +  + K+K ENK+KFA +++K  ++ ++P S+FD QVKRIHEYKRQ+LN LH I LYNR+K   +   +PRTVMIGGKAAPGYH+AK+ IKLI+ VG+++NNDP + +KL+++FLENYRV+LAE++ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYQVDPDKFQNKTNGITPRRWLLLCNPGLADIIAERIGEDFLTDLYQLKNILQFVDDEAFIRDVAKVKQENKLKFAAFLQKHCQVKVNPESIFDVQVKRIHEYKRQLLNCLHAITLYNRIKLRPERAFVPRTVMIGGKAAPGYHMAKMIIKLITSVGEVVNNDPAVGDKLKVIFLENYRVSLAEQVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygl (glycogen phosphorylase L [Source:NCBI gene;Acc:101158492])

HSP 1 Score: 268.855 bits (686), Expect = 1.145e-84
Identity = 127/219 (57.99%), Postives = 175/219 (79.91%), Query Frame = 1
Query:   22 QTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            +T  F+DF EL P KFQNKTNGITPRRWLLLCNP L++ +  +IG+ ++ +LS L +L +F      +  + K+K +NK+KFA Y++K+Y++ I+  S+FD  VKRIHEYKRQ+LN LH+I LYNR++ N     +PRTV+IGGKAAPGY++AK+ IKLI+ V  ++NNDP++ +KL++++LENYRV+LAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  463 KTQVFRDFSELEPGKFQNKTNGITPRRWLLLCNPGLAELIAEVIGEDYVKDLSQLRKLNDFVNNAAFIRDVSKVKQDNKLKFAQYLEKEYRVKINLASMFDVHVKRIHEYKRQLLNCLHIIALYNRIRKNPAAPFVPRTVIIGGKAAPGYYMAKMIIKLITSVADVVNNDPVVGSKLKVIYLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFM 681          
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Match: pygma (glycogen phosphorylase, muscle form [Source:NCBI gene;Acc:101169133])

HSP 1 Score: 268.47 bits (685), Expect = 1.545e-84
Identity = 129/215 (60.00%), Postives = 172/215 (80.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYE+ P+KFQNKTNGITPRRWL++CNP L++ +   IG+ +I +L  L  L  F  ++  +  + K+K ENKMKFA ++++ YK+ I+ +S+FD QVKRIHEYKRQ+LN LH+I  YNR+K        PRTVMIGGKAAPGYH AK+ I+LI+ +G++INNDPI+ ++L+++FLENY+VTLAEK+ PA++LS+QISTAGTEASGTGNMKFM
Sbjct:  469 FKDFYEMEPHKFQNKTNGITPRRWLVMCNPGLAEVIAEKIGEDFIRDLDQLQALRNFVNDEAFIRDVAKVKQENKMKFAVHLEEHYKVKINRDSMFDVQVKRIHEYKRQLLNCLHMITYYNRIKKEPSKQWTPRTVMIGGKAAPGYHTAKMIIRLITAIGEVINNDPIVGDRLKIIFLENYKVTLAEKVIPAADLSEQISTAGTEASGTGNMKFM 683          
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 416.001 bits (1068), Expect = 1.445e-142
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM
Sbjct:  430 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 644          
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 416.001 bits (1068), Expect = 3.599e-142
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM
Sbjct:  464 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678          
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000013460.1 (SMESG000013460.1)

HSP 1 Score: 416.001 bits (1068), Expect = 1.211e-141
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM
Sbjct:  464 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678          
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000061417.1 (SMESG000061417.1)

HSP 1 Score: 305.834 bits (782), Expect = 5.769e-99
Identity = 149/215 (69.30%), Postives = 184/215 (85.58%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            FKDF +LWP KFQNKTNGITPRRWLLLCNPNL+DY+  +IGDSWI NL+DLS+++     + V+  +++IK ENK KFA Y++K   I IDPN++FD QVKRIHEYKRQ+LN L+VIV+YNRLK N   D+ PRTVMIGGKAAPGYH+AKL IKLI++V  +IN+DP+++ KL++V+LENYRV+LAEKIFPA++LSQQISTAGTEASGTGNMKFM
Sbjct:  465 FKDFSDLWPEKFQNKTNGITPRRWLLLCNPNLADYINEIIGDSWIQNLNDLSKMKLLDKHEQVLRSLMRIKRENKAKFAEYIEKHMHIKIDPNTMFDIQVKRIHEYKRQLLNCLYVIVMYNRLKMNPTLDIAPRTVMIGGKAAPGYHMAKLIIKLINQVALVINHDPVVRKKLKVVYLENYRVSLAEKIFPAADLSQQISTAGTEASGTGNMKFM 679          
BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Match: SMESG000042275.1 (SMESG000042275.1)

HSP 1 Score: 303.138 bits (775), Expect = 5.223e-98
Identity = 147/215 (68.37%), Postives = 179/215 (83.26%), Query Frame = 1
Query:   34 FKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISLIGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKINIDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIGGKAAPGYHIAKLTIKLISEVGXXXXXXXXXXXXLRLVFLENYRVTLAEKIFPASELSQQISTAGTEASGTGNMKFM 678
            F+DF + WP +FQNKTNGITPRRWLLLCNP+LS+Y+   IG SWI NL+DLS L+ F  +++V+  I++IK ENK+KFA Y++K   I I+PN++FDFQVKRIHEYKRQ+LN L+VIVLYNRLK N   ++ PRTVM GGKAAPGYH+AKL IKLI++V   I  DP I  KL+LVFLENYRV++AEKIFPA++LSQQISTAGTEASGTGNMKFM
Sbjct:  452 FRDFSDFWPERFQNKTNGITPRRWLLLCNPDLSNYISQNIGRSWINNLNDLSDLKRFCDDENVLESIMQIKYENKVKFASYIEKNMNIKINPNTMFDFQVKRIHEYKRQLLNCLYVIVLYNRLKKNPHLEITPRTVMFGGKAAPGYHMAKLIIKLINQVASTIYQDPAISEKLKLVFLENYRVSMAEKIFPAADLSQQISTAGTEASGTGNMKFM 666          
The following BLAST results are available for this feature:
BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PYGM7.025e-9261.86glycogen phosphorylase, muscle associated [Source:... [more]
PYGM4.579e-9161.86glycogen phosphorylase, muscle associated [Source:... [more]
PYGB1.831e-9061.40glycogen phosphorylase B [Source:HGNC Symbol;Acc:H... [more]
PYGL4.969e-8961.40glycogen phosphorylase L [Source:HGNC Symbol;Acc:H... [more]
PYGL5.685e-8961.40glycogen phosphorylase L [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pygl-11.654e-9160.93Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-13.995e-8960.93Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-15.237e-8960.93Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
pygl-13.307e-3168.24Alpha-1,4 glucan phosphorylase [Source:UniProtKB/... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GlyP4.732e-8561.68gene:FBgn0004507 transcript:FBtr0100485[more]
GlyP4.732e-8561.68gene:FBgn0004507 transcript:FBtr0077828[more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pygmb1.168e-8860.93phosphorylase, glycogen, muscle b [Source:ZFIN;Acc... [more]
pygmb1.168e-8860.93phosphorylase, glycogen, muscle b [Source:ZFIN;Acc... [more]
pygl1.402e-8861.86phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZD... [more]
pygl2.238e-8861.86phosphorylase, glycogen, liver [Source:ZFIN;Acc:ZD... [more]
pygma2.328e-8760.00phosphorylase, glycogen, muscle A [Source:ZFIN;Acc... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
taf82.950e-9464.65TATA-box binding protein associated factor 8 [Sour... [more]
taf81.557e-9364.65TATA-box binding protein associated factor 8 [Sour... [more]
taf82.563e-9364.65TATA-box binding protein associated factor 8 [Sour... [more]
taf82.602e-9364.65TATA-box binding protein associated factor 8 [Sour... [more]
taf82.618e-9364.65TATA-box binding protein associated factor 8 [Sour... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pygm5.536e-9261.40muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm5.536e-9261.40muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm2.713e-9161.40muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygm2.713e-9161.40muscle glycogen phosphorylase [Source:MGI Symbol;A... [more]
Pygl7.082e-9061.19liver glycogen phosphorylase [Source:MGI Symbol;Ac... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P00489|PYGM_RABIT1.757e-9062.33Glycogen phosphorylase, muscle form OS=Oryctolagus... [more]
sp|Q9WUB3|PYGM_MOUSE1.898e-9061.40Glycogen phosphorylase, muscle form OS=Mus musculu... [more]
sp|P11217|PYGM_HUMAN2.199e-9061.86Glycogen phosphorylase, muscle form OS=Homo sapien... [more]
sp|P79334|PYGM_BOVIN5.495e-9061.86Glycogen phosphorylase, muscle form OS=Bos taurus ... [more]
sp|P11216|PYGB_HUMAN8.792e-9061.40Glycogen phosphorylase, brain form OS=Homo sapiens... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183KLL03.082e-10066.06Alpha-1,4 glucan phosphorylase OS=Schistosoma cura... [more]
A0A3P7YSD34.144e-9866.06Alpha-1,4 glucan phosphorylase OS=Schistosoma marg... [more]
A0A183M3P74.572e-9866.06Alpha-1,4 glucan phosphorylase OS=Schistosoma marg... [more]
A0A093IXG65.228e-9763.72Alpha-1,4 glucan phosphorylase (Fragment) OS=Euryp... [more]
Q5BY061.110e-9666.52Alpha-1,4 glucan phosphorylase (Fragment) OS=Schis... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pygmb1.061e-8760.93glycogen phosphorylase, muscle form-like [Source:N... [more]
pygmb5.459e-8760.93glycogen phosphorylase, muscle form-like [Source:N... [more]
pygmb5.459e-8760.93glycogen phosphorylase, muscle form-like [Source:N... [more]
pygma2.077e-8659.53phosphorylase, glycogen, muscle A [Source:ZFIN;Acc... [more]
pygma5.061e-8659.53phosphorylase, glycogen, muscle A [Source:ZFIN;Acc... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000006065.14.115e-8961.86pep scaffold:Pmarinus_7.0:GL476342:314148:364539:-... [more]
ENSPMAT00000008899.11.609e-8759.07pep scaffold:Pmarinus_7.0:GL479616:11553:30183:-1 ... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GPH14.791e-4844.96Glycogen phosphorylase required for the mobilizati... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO338113.022e-6052.31Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7SR65... [more]
EDO277711.174e-777.78Phosphorylase [Source:UniProtKB/TrEMBL;Acc:A7T8B9... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pygmb6.218e-8759.53glycogen phosphorylase, muscle associated [Source:... [more]
pygb8.873e-8760.00glycogen phosphorylase B [Source:NCBI gene;Acc:101... [more]
pygl1.145e-8457.99glycogen phosphorylase L [Source:NCBI gene;Acc:101... [more]
pygma1.545e-8460.00glycogen phosphorylase, muscle form [Source:NCBI g... [more]
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BLAST of Alpha-1,4 glucan phosphorylase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000013460.11.445e-142100.00SMESG000013460.1[more]
SMESG000013460.13.599e-142100.00SMESG000013460.1[more]
SMESG000013460.11.211e-141100.00SMESG000013460.1[more]
SMESG000061417.15.769e-9969.30SMESG000061417.1[more]
SMESG000042275.15.223e-9868.37SMESG000042275.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005829 ID=SMED30005829|Name=Alpha-1,4 glucan phosphorylase|organism=Schmidtea mediterranea sexual|type=transcript|length=678bp
ATGGATTATTTACAAAATAGACAAACCTTCAAGTTCAAAGATTTTTACGA
ATTATGGCCGAATAAATTTCAAAATAAAACGAATGGAATTACTCCAAGAA
GATGGCTTTTGTTATGTAACCCAAATTTGTCAGATTATTTAATAAGTTTG
ATAGGAGATTCTTGGATCACTAACTTGAGTGATTTGAGTCGATTAGAAGA
GTTTTACGGGGAAAAAGATGTGGTGCATCAAATTTTGAAGATAAAAGCCG
AAAATAAAATGAAATTTGCGCATTATATGAAAAAACAATATAAAATTAAT
ATAGATCCAAACAGTTTATTCGATTTTCAGGTCAAAAGAATACACGAATA
TAAAAGACAGATGCTCAATGTGCTCCACGTAATTGTTCTCTATAATAGAT
TAAAGTCTAACTCAAAATATGACATGATCCCAAGAACGGTTATGATTGGA
GGAAAGGCCGCACCGGGTTACCATATTGCCAAATTGACTATCAAGTTGAT
CAGTGAAGTGGGAAAGATCATTAACAATGATCCAATAATTAAAAATAAAT
TGCGACTGGTATTTCTTGAAAATTATCGCGTAACACTGGCAGAAAAGATA
TTTCCAGCTAGTGAATTATCCCAGCAAATATCCACTGCCGGTACTGAAGC
TTCAGGGACGGGCAACATGAAATTTATG
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protein sequence of SMED30005829-orf-1

>SMED30005829-orf-1 ID=SMED30005829-orf-1|Name=SMED30005829-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=226bp
MDYLQNRQTFKFKDFYELWPNKFQNKTNGITPRRWLLLCNPNLSDYLISL
IGDSWITNLSDLSRLEEFYGEKDVVHQILKIKAENKMKFAHYMKKQYKIN
IDPNSLFDFQVKRIHEYKRQMLNVLHVIVLYNRLKSNSKYDMIPRTVMIG
GKAAPGYHIAKLTIKLISEVGKIINNDPIIKNKLRLVFLENYRVTLAEKI
FPASELSQQISTAGTEASGTGNMKFM
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
GO:0008184glycogen phosphorylase activity
GO:0004645phosphorylase activity
GO:0030170pyridoxal phosphate binding
Vocabulary: INTERPRO
TermDefinition
IPR000811Glyco_trans_35
Vocabulary: biological process
TermDefinition
GO:0005975carbohydrate metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.2000coord: 31..222
e-value: 5.4E-91
score: 307.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.2000coord: 3..30
e-value: 1.1E-5
score: 26.0
NoneNo IPR availablePANTHERPTHR11468:SF13GLYCOGEN PHOSPHORYLASE, MUSCLE FORMcoord: 7..222
NoneNo IPR availableCDDcd04300GT35_Glycogen_Phosphorylasecoord: 8..222
e-value: 3.45969E-141
score: 411.535
NoneNo IPR availableSUPERFAMILYSSF53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 6..222
IPR000811Glycosyl transferase, family 35PFAMPF00343Phosphorylasecoord: 7..222
e-value: 5.9E-104
score: 348.8
IPR000811Glycosyl transferase, family 35PANTHERPTHR11468GLYCOGEN PHOSPHORYLASEcoord: 7..222