SMED30005816

Overview
NameSMED30005816
Smed IDSMED30005816
Length (bp)999
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005816 (SMED30005816) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005816 (SMED30005816) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005816 (SMED30005816) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005816 (SMED30005816) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005816

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30005816h1SMcG0006466 Contig55416newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
ovarySMED30005816h1SMcG0001937 Contig1294uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005816h1SMcG0001937 Contig1294newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
vitelline glandSMED30005816h1SMcG0001937 Contig1294uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
vitelline glandSMED30005816h1SMcG0001937 Contig1294newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005816h1SMcG0006466 Contig1294uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005816h1SMcG0006466 Contig1294newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
vitelline glandSMED30005816h1SMcG0006466 Contig1294uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
vitelline glandSMED30005816h1SMcG0006466 Contig1294newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005816 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.008857:11015..17446 +4726
Homology
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BLAST of SMED30005816 vs. TrEMBL
Match: A0A0X3PE25 (DUF4477 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=TR86979 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 5.908e-6
Identity = 53/197 (26.90%), Postives = 84/197 (42.64%), Query Frame = 2 
Query:    5 KKFDSYPSLLYGHIRDIRSIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLH---KVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINS 586
            K  D  PS++   +R++ +I  L         DL+  +    KYR +  H+ +F  LC++ R++R        F   FF +K  +     + P  N  D LLL + N     +V   L + C  L E     M TGH      + +T  + I   C+  +    N Y  +Y +RE +P    S  S  + LPT I +
Sbjct:   10 KSVDCDPSVVDFLVRNVDTIRPLLATWSAENQDLSILKALTYKYRNQQRHFPYFLSLCHIERRLRKTFHGSSRFGIDFFLQKFRQ----VKCPNRNCLDYLLLSLCNWRQELRVTRSLAVTCWKLCE---RQMLTGHFVKLMMVVMTVIARILIMCELTIATTANIYNSLYAMRERIP-VPASLASYLDDLPTHIEA 198
[Close]
BLAST of SMED30005816 vs. TrEMBL
Match: A0A0X3NZB3 (DUF4477 domain-containing protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR86979 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 6.062e-6
Identity = 49/194 (25.26%), Postives = 83/194 (42.78%), Query Frame = 2 
Query:    5 KKFDSYPSLLYGHIRDIRSIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINS 586
            K  D  PS++   +R++ +I  L         DL+  +    KYR +  H+ +F  LC++ R++R        F   FF +K  +     + P  N  D LLL + N  +         ++ ++L    M TGH      + +T  + I   C+  +    N Y  +Y +RE +P    S  S  + LPT I +
Sbjct:   39 KSVDCDPSVVDFLVRNVDTIRPLLATWSAENQDLSILKALTYKYRNQQRHFPYFLSLCHIERRLRKTFHGSSRFGIDFFLQKFRQ----VKCPNRNCLDYLLLSLCNWRQELRVTRSLAVTCWKLCERQMLTGHFVKLMMVVMTVIARILIMCELTIATTANIYNSLYAMRERIP-VPASLASYLDDLPTHIEA 227
[Close]
BLAST of SMED30005816 vs. TrEMBL
Match: A0A0X3PF42 (DUF4477 domain-containing protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR86979 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 6.760e-6
Identity = 49/194 (25.26%), Postives = 83/194 (42.78%), Query Frame = 2 
Query:    5 KKFDSYPSLLYGHIRDIRSIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINS 586
            K  D  PS++   +R++ +I  L         DL+  +    KYR +  H+ +F  LC++ R++R        F   FF +K  +     + P  N  D LLL + N  +         ++ ++L    M TGH      + +T  + I   C+  +    N Y  +Y +RE +P    S  S  + LPT I +
Sbjct:   59 KSVDCDPSVVDFLVRNVDTIRPLLATWSAENQDLSILKALTYKYRNQQRHFPYFLSLCHIERRLRKTFHGSSRFGIDFFLQKFRQ----VKCPNRNCLDYLLLSLCNWRQELRVTRSLAVTCWKLCERQMLTGHFVKLMMVVMTVIARILIMCELTIATTANIYNSLYAMRERIP-VPASLASYLDDLPTHIEA 247
[Close]
BLAST of SMED30005816 vs. Planmine SMEST
Match: SMESG000022353.1 (SMESG000022353.1)

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 313/328 (95.43%), Postives = 313/328 (95.43%), Query Frame = 2 
Query:    2 MKKFDSYPSLLYGHIRDIR--------------SIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINSLSALYKERVEMKNFQSIPITTESDIVIVNKESQWKPIQTIESTNVAEPXXXXXXXXXXVLAATENVSTLVDNVPDFDVGVXXXXXXXXXXXXXXXXXXEEKQFLKNNKKRKIGESALNF 943
            MKKFDSYPSLLYGHIRDI               SIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINSLSALYKERVEMKNFQSIPITTESDIVIVNKESQWKPIQTIESTNVAEPSSKKRKRKSKVLAATENVSTLVDNVPDFDVGVSKKQKKKMKQEMNSKIKKEEKQFLKNNKKRKIGESALNF
Sbjct:    1 MKKFDSYPSLLYGHIRDISRNLFLVQFYFFLLGSIEFLKGVNINLYLDLNTFELFIKKYRTRDHHYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWDCLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSIIGTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINSLSALYKERVEMKNFQSIPITTESDIVIVNKESQWKPIQTIESTNVAEPSSKKRKRKSKVLAATENVSTLVDNVPDFDVGVSKKQKKKMKQEMNSKIKKEEKQFLKNNKKRKIGESALNF 328
The following BLAST results are available for this feature:
BLAST of SMED30005816 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A0X3PE255.908e-626.90DUF4477 domain-containing protein OS=Schistocephal... [more]
A0A0X3NZB36.062e-625.26DUF4477 domain-containing protein (Fragment) OS=Sc... [more]
A0A0X3PF426.760e-625.26DUF4477 domain-containing protein (Fragment) OS=Sc... [more]
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BLAST of SMED30005816 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005816 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000022353.10.000e+095.43SMESG000022353.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005816 ID=SMED30005816|Name=SMED30005816|organism=Schmidtea mediterranea sexual|type=transcript|length=999bp
CATGAAAAAATTCGATTCTTATCCTTCTCTTTTATATGGTCACATTCGAG
ATATAAGAAGTATAGAATTTTTAAAAGGTGTAAATATCAATTTATATTTG
GATCTTAACACATTTGAATTATTTATTAAAAAATATCGAACAAGAGATCA
TCATTATGTCTGGTTCAAAAAGCTATGCAATTTAATTCGACAAATTCGCA
ATATCAGAGGTGATCAGATAGAGTTTGTTCAAACATTTTTTTCTGAAAAA
CTTTCAAAAAATTTAGACTCTTGTCAGGTACCGACGAGTAATTATTGGGA
TTGCCTTTTGTTATGCATGATAAATTTACACAAAGTTTATTATGAACTAT
TTTTACATTGCATTTCATTATTCGAATTACTTACCGCGACTATGAAGACT
GGACATATTAGAGCTCGTTGTGCATTGGGTATTACTTTTACATCAATTAT
CGGAACCTTTTGCAAGACAGTTTTGGAATTTTTCGAAAATAATTACGGAA
AAATTTATGAGATCAGAGAAAGTTTACCTAAATCCAAAACATCATGGTTG
TCAGTCGAAAATTCTTTACCAACAGTTATAAATAGTCTGAGTGCACTTTA
TAAAGAACGAGTAGAAATGAAGAACTTTCAGAGTATTCCGATCACAACGG
AGTCTGATATTGTAATTGTAAATAAAGAATCACAATGGAAACCAATTCAA
ACAATAGAGAGCACAAATGTTGCAGAGCCTTCGTCTAAGAAACGGAAGCG
GAAATCCAAAGTTTTAGCTGCCACAGAAAATGTGTCGACATTGGTCGATA
ATGTTCCCGATTTTGATGTTGGAGTTTCCAAAAAGCAAAAGAAGAAAATG
AAACAAGAAATGAACTCTAAAATAAAAAAGGAGGAAAAACAATTTCTGAA
AAATAATAAGAAACGGAAAATCGGTGAAAGTGCTCTTAATTTTCTATCGT
AATAATCATTGTTGTAATGAATAAATTTTTTTGGTGTCTTTCTTTTGGG
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protein sequence of SMED30005816-orf-1

>SMED30005816-orf-1 ID=SMED30005816-orf-1|Name=SMED30005816-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=317bp
MKKFDSYPSLLYGHIRDIRSIEFLKGVNINLYLDLNTFELFIKKYRTRDH
HYVWFKKLCNLIRQIRNIRGDQIEFVQTFFSEKLSKNLDSCQVPTSNYWD
CLLLCMINLHKVYYELFLHCISLFELLTATMKTGHIRARCALGITFTSII
GTFCKTVLEFFENNYGKIYEIRESLPKSKTSWLSVENSLPTVINSLSALY
KERVEMKNFQSIPITTESDIVIVNKESQWKPIQTIESTNVAEPSSKKRKR
KSKVLAATENVSTLVDNVPDFDVGVSKKQKKKMKQEMNSKIKKEEKQFLK
NNKKRKIGESALNFLS*
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InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 277..297
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 276..302