N-methylpurine-DNA glycosylase

Overview
NameN-methylpurine-DNA glycosylase
Smed IDSMED30005798
Length (bp)797
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of N-methylpurine-DNA glycosylase (SMED30005798) t-SNE clustered cells

Violin plots show distribution of expression levels for N-methylpurine-DNA glycosylase (SMED30005798) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of N-methylpurine-DNA glycosylase (SMED30005798) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for N-methylpurine-DNA glycosylase (SMED30005798) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005798

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005798SMESG000067444.1 SmedASXL_000354SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Human
Match: MPG (N-methylpurine DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:7211])

HSP 1 Score: 146.747 bits (369), Expect = 3.656e-42
Identity = 76/208 (36.54%), Postives = 124/208 (59.62%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRK------LKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE----DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L   FFD     LA+  LG++LVR   + + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+NI+ +  G  VL+R+LEP+EG++ M + R          +  D++LC+GP KLCQ+L + K F+Q+++     +W+E    + ++   V  A     +  GE A   L R+Y++G+ +VS +++
Sbjct:   68 RLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHA-GEWARKPL-RFYVRGSPWVSVVDR 273
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Human
Match: MPG (N-methylpurine DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:7211])

HSP 1 Score: 147.132 bits (370), Expect = 4.608e-42
Identity = 76/208 (36.54%), Postives = 124/208 (59.62%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRK------LKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE----DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L   FFD     LA+  LG++LVR   + + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+NI+ +  G  VL+R+LEP+EG++ M + R          +  D++LC+GP KLCQ+L + K F+Q+++     +W+E    + ++   V  A     +  GE A   L R+Y++G+ +VS +++
Sbjct:   85 RLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHA-GEWARKPL-RFYVRGSPWVSVVDR 290
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Human
Match: MPG (N-methylpurine DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:7211])

HSP 1 Score: 146.747 bits (369), Expect = 5.152e-42
Identity = 76/208 (36.54%), Postives = 124/208 (59.62%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRK------LKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE----DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L   FFD     LA+  LG++LVR   + + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+NI+ +  G  VL+R+LEP+EG++ M + R          +  D++LC+GP KLCQ+L + K F+Q+++     +W+E    + ++   V  A     +  GE A   L R+Y++G+ +VS +++
Sbjct:   80 RLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHA-GEWARKPL-RFYVRGSPWVSVVDR 285
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Human
Match: MPG (N-methylpurine DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:7211])

HSP 1 Score: 140.969 bits (354), Expect = 3.771e-40
Identity = 65/161 (40.37%), Postives = 101/161 (62.73%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRK------LKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE 618
            +L   FFD     LA+  LG++LVR   + + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+NI+ +  G  VL+R+LEP+EG++ M + R          +  D++LC+GP KLCQ+L + K F+Q+++     +W+E
Sbjct:   68 RLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLE 228
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Zebrafish
Match: mpg (N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZDB-GENE-070410-121])

HSP 1 Score: 166.007 bits (419), Expect = 1.167e-49
Identity = 93/209 (44.50%), Postives = 130/209 (62.20%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS-----DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE-DCN-----DTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L  SFF+     LAK  LGK+LVR   D + + GKI+ETE+Y G  D ASHS  G+ T RN AMFM  GT+Y Y IYG+  C+N++ +  G AVL+RSLEP+ G D M   R  K         DK+LCNGP KLCQ+L++ + F+++++     +W+E D       D  +VV A RIGV+ +GE A+  L R+Y++G+  VS +NK
Sbjct:   69 RLTYSFFNQPCVELAKAFLGKVLVRKLTDGTELRGKIVETEAYLGGEDKASHSAGGKRTERNTAMFMKPGTIYVYPIYGIYLCMNVSSQGEGAAVLLRSLEPLSGQDVMRGLRAAKRKPGAKSLKDKELCNGPSKLCQALDIQRSFDRRDLATDVEVWLEMDPEKEAIVDAGEVVMAPRIGVDSHGEWATKPL-RFYLRGHPCVSVLNK 276
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Xenopus
Match: mpg (N-methylpurine DNA glycosylase [Source:NCBI gene;Acc:549932])

HSP 1 Score: 172.555 bits (436), Expect = 3.923e-52
Identity = 99/242 (40.91%), Postives = 146/242 (60.33%), Query Frame = 1 
Query:   37 IIYNISCSFLVKMKRRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYR------KLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTF--DVVNASRIGVNGYGEKASNCLYRYYIKGNSYVS 735
            I+ N  C  ++     K + S  S +     S    +P + L   F++     LAK+ LG++LVR   D + + G+I+ETESY G +D ASHS  G+ T RN AM+M  GT+Y Y IYG+  C+N++ +  G AVL+RSLEP+EG+D M  +R      K K ++ + +LCNGP KLCQ+L++ K +++K++ N  + WIE  +  F  D+V+ SRIG+   GE     L R+YIKGN YVS
Sbjct:   18 ILNNAPCKMILHESTEKHETSSYSEEQKPKISKYFQNPGIHLLSEFYNQPCTELAKSFLGQVLVRKLPDGTELRGRIVETESYLGGDDEASHSRGGKRTERNVAMYMKPGTIYVYQIYGIYFCMNVSSQGDGAAVLLRSLEPLEGLDIMRNFRNGRRNEKAKPLK-ETELCNGPSKLCQALDINKSYDRKDLTNDQDTWIEAGSKIFDEDIVSCSRIGIGNAGEWTKKPL-RFYIKGNKYVS 257
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Xenopus
Match: mpg (N-methylpurine DNA glycosylase [Source:NCBI gene;Acc:549932])

HSP 1 Score: 172.17 bits (435), Expect = 4.683e-52
Identity = 99/242 (40.91%), Postives = 146/242 (60.33%), Query Frame = 1 
Query:   37 IIYNISCSFLVKMKRRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYR------KLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTF--DVVNASRIGVNGYGEKASNCLYRYYIKGNSYVS 735
            I+ N  C  ++     K + S  S +     S    +P + L   F++     LAK+ LG++LVR   D + + G+I+ETESY G +D ASHS  G+ T RN AM+M  GT+Y Y IYG+  C+N++ +  G AVL+RSLEP+EG+D M  +R      K K ++ + +LCNGP KLCQ+L++ K +++K++ N  + WIE  +  F  D+V+ SRIG+   GE     L R+YIKGN YVS
Sbjct:   10 ILNNAPCKMILHESTEKHETSSYSEEQKPKISKYFQNPGIHLLSEFYNQPCTELAKSFLGQVLVRKLPDGTELRGRIVETESYLGGDDEASHSRGGKRTERNVAMYMKPGTIYVYQIYGIYFCMNVSSQGDGAAVLLRSLEPLEGLDIMRNFRNGRRNEKAKPLK-ETELCNGPSKLCQALDINKSYDRKDLTNDQDTWIEAGSKIFDEDIVSCSRIGIGNAGEWTKKPL-RFYIKGNKYVS 249
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Mouse
Match: Mpg (N-methylpurine-DNA glycosylase [Source:MGI Symbol;Acc:MGI:97073])

HSP 1 Score: 171.014 bits (432), Expect = 4.631e-51
Identity = 84/211 (39.81%), Postives = 134/211 (63.51%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNI-------RS--DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE----DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L P FFD    +LA+  LG++LVR   D + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+N++ +  G  VL+R+LEP+EG++ M   R+L+N        RS  D++LC+GP KLCQ+L + K F+Q+++   + +W+E    + +    VV A+RIG+   GE     L R+Y++G+ +VS +++
Sbjct:  105 RLGPEFFDQPAVTLARAFLGQVLVRRLADGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGLETM---RQLRNSLRKSTVGRSLKDRELCSGPSKLCQALAIDKSFDQRDLAQDDAVWLEHGPLESSSPAVVVAAARIGIGHAGEWTQKPL-RFYVQGSPWVSVVDR 311
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Match: sp|Q04841|3MG_MOUSE (DNA-3-methyladenine glycosylase OS=Mus musculus OX=10090 GN=Mpg PE=2 SV=3)

HSP 1 Score: 171.014 bits (432), Expect = 3.239e-50
Identity = 84/211 (39.81%), Postives = 134/211 (63.51%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNI-------RS--DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIE----DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L P FFD    +LA+  LG++LVR   D + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+N++ +  G  VL+R+LEP+EG++ M   R+L+N        RS  D++LC+GP KLCQ+L + K F+Q+++   + +W+E    + +    VV A+RIG+   GE     L R+Y++G+ +VS +++
Sbjct:  105 RLGPEFFDQPAVTLARAFLGQVLVRRLADGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGLETM---RQLRNSLRKSTVGRSLKDRELCSGPSKLCQALAIDKSFDQRDLAQDDAVWLEHGPLESSSPAVVVAAARIGIGHAGEWTQKPL-RFYVQGSPWVSVVDR 311
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Match: sp|P23571|3MG_RAT (DNA-3-methyladenine glycosylase (Fragment) OS=Rattus norvegicus OX=10116 GN=Mpg PE=2 SV=2)

HSP 1 Score: 157.532 bits (397), Expect = 3.424e-45
Identity = 82/234 (35.04%), Postives = 141/234 (60.26%), Query Frame = 1 
Query:   79 RRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNI-------RS--DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCN-DTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            + K+ ++   ++S+ F S +  D P +L P +FD    +LA+  LG++LVR   D + + G+I+ETE+Y G  D A+HS  G +T RN+ MFM  GT+Y Y IYG+  C+N++ +  G  VL+R+LEP+EG++ M   R+L+N        RS  D++LCNGP KLCQ+L  +K F+Q+++     +W+E    ++      +     G+  + +    R+Y++G+ +VS +++
Sbjct:   67 KEKLSSTPGLLRSIYFSSPE--DRPARLGPEYFDQPAVTLARAFLGQVLVRRLADGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGLETM---RQLRNSLRKSTVGRSLKDRELCNGPSKLCQALARSKSFDQRDLAQDEAVWLEHGPLESSSPAVVAAAAGIGHAGEWTQKPLRFYVQGSPWVSVVDR 295
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Match: sp|Q0SPY0|3MGH_CLOPS (Putative 3-methyladenine DNA glycosylase OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=CPR_2580 PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.808e-44
Identity = 86/206 (41.75%), Postives = 126/206 (61.17%), Query Frame = 1 
Query:  154 LKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKE--IGTAVLIRSLEPVEGIDKMIKYRKLKNIR-----SDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIED---CNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSK 738
            ++L   F++ D  ++AK LLGK+LVR N +   + GKI+ETE+Y G  D ASH+Y G+ T R + ++   GTVY Y IYG+ HC+N+  +E  +   VLIR +EP+EGI++M K R  KN         K   NGP KLC +L + K  N  N  +   +++ED     + F +V A RIG++ Y E+A + L+R+YIK N +VSK
Sbjct:    1 MRLGRDFYNRDTLTVAKELLGKVLVR-NINGVTLKGKIVETEAYIGAIDKASHAYGGKRTNRTETLYADPGTVYVYIIYGMYHCLNLISEEKDVAGGVLIRGIEPLEGIEEMSKLRYRKNYEELSSYEKKNFSNGPSKLCMALGIDKGENGINTISSEEIYVEDDSSIKEDFSIVEAKRIGID-YAEEARDFLWRFYIKDNKFVSK 204
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Match: sp|A0PZK9|3MGH_CLONN (Putative 3-methyladenine DNA glycosylase OS=Clostridium novyi (strain NT) OX=386415 GN=NT01CX_1732 PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 3.929e-44
Identity = 85/202 (42.08%), Postives = 133/202 (65.84%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINI--TGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS-----DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSK 738
            +LN  F+  D   +AK+LLGKI+V  N+  +++ GKI+E E+Y G++D A+HSY   +T R K M+   G VY + IYG+ +C+NI  + K++  AVLIR++EP+E ID   K R  K+         K + NGP KLC ++N+TK+FN  ++C  +N++I D  + F++V + RIG++ Y E+A + L+R+YIK + YVSK
Sbjct:    3 RLNREFYTRDTIEVAKDLLGKIIVVENE--TKLLGKIVEVEAYGGISDKAAHSYGNRKTERTKIMYEEGGYVYVFQIYGMYNCLNIVSSKKDVPEAVLIRAVEPIENIDDFSKNRYGKDFNELTKYQQKNITNGPGKLCMAMNITKKFNGLDLCK-DNIYIVDNKEEFEIVASKRIGID-YAEEAKDYLWRFYIKDSKYVSK 200
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Match: sp|Q97EY6|3MGH_CLOAB (Putative 3-methyladenine DNA glycosylase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_C2969 PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.813e-43
Identity = 85/208 (40.87%), Postives = 133/208 (63.94%), Query Frame = 1 
Query:  154 LKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIG--TAVLIRSLEPVEGIDKMIKYRKLKNIRSD------KQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDC-NDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +KL   F+  D   +AK LLGK+LV    +  R SGKI+E E+Y G+ND  +H+Y G  T R +A++  +G  Y Y+IYGL +C+N+   + G    VLIR++EP+EGI+ M + R+ K + +D      K L NGP KLC ++ + +E N  ++ N + L+IE+  N++F++V A R+G++ Y E+A + L+R+YIKGN  VS + K
Sbjct:    1 MKLIREFYSRDTIVVAKELLGKVLVH-EVNGIRTSGKIVEVEAYRGINDKGAHAYGGRRTPRTEALYGPAGHAYVYFIYGLYYCMNVVAMQEGIPEGVLIRAIEPIEGIEVMSE-RRFKKLFNDLTKYQLKNLTNGPSKLCSAMEIRREQNLMDL-NGDELYIEEGKNESFEIVEAKRVGID-YAEEAKDYLWRFYIKGNKCVSVLKK 204
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Match: K7J9C9 (Uncharacterized protein OS=Nasonia vitripennis OX=7425 GN=100119302 PE=3 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 5.500e-55
Identity = 97/244 (39.75%), Postives = 151/244 (61.89%), Query Frame = 1 
Query:   85 KVDNSIISMKSMKFQSVDIIDPPL----------KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDK----------QLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIED--CNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            K   +++ +K MK +   + DPP+          +L  SF+DV   +LAK++LGK+LVR  ++ + + G+I+ETESY G+ D ASH++KG+ T RN  M+M  GT+Y Y+ YG+ HC NI+ +E G+AVL+R+LEP+EGID+M ++R LK    ++          +LCNGP K+C +L L K+ ++ ++C+   LW+ED    +   +V   RIG+   G + S    RYYI G+  VSK +K
Sbjct:   46 KKPRAVVDLKLMKEELKQLEDPPMTPWEKKISSNRLPYSFYDVPCKTLAKSILGKVLVRKLENGTILKGRIVETESYLGIEDGASHTFKGKVTPRNTPMYMKPGTIYVYFTYGMYHCFNISSQEEGSAVLVRALEPLEGIDQMAQHRSLKPGAKEQKKLSKELKTHELCNGPSKICMALQLEKQHSKYSMCSWKELWLEDDGTKEEIKIVECPRIGIESSGVEWSQKPLRYYIYGHKCVSKRDK 289
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Match: T1JKX3 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=3 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 1.391e-54
Identity = 98/202 (48.51%), Postives = 138/202 (68.32%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYR--KLKNIRSDK--QLCNGPVKLCQSLNLTKE-FNQKNICNLNNLWIEDCNDTFD--VVNASRIGVNGYGEKASNCLYRYYIKGNSYVSK 738
            +L   FF V   +LA+ LLG++LVRI DD +R+SG I+ETE Y G  D ASHSY G+ T RN AMFM  GT Y Y++YG+ HC NI+ +  G+AVLIRSL+P+EG+D+M ++R  +LKN R+ K  +LCNGP KLC S+ +TK+  +Q+++     LW+E   +T D  +  ASRIG+N  G + +    R+Y+KG  YVS+
Sbjct:    4 RLGKDFFTVTCQTLARKLLGQVLVRILDDGTRLSGVIVETECYLGGEDKASHSYNGKRTERNTAMFMDPGTAYVYFVYGMYHCFNISSEGDGSAVLIRSLQPLEGLDQMQQFRNERLKNQRALKCDKLCNGPSKLCMSMAITKDVIDQRDLTVCEFLWVEKGWETPDSCIAVASRIGINSAGAEWAEKPLRFYVKGCKYVSR 205
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Match: A0A087SV84 (DNA-3-methyladenine glycosylase (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_09420 PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 6.123e-54
Identity = 99/226 (43.81%), Postives = 144/226 (63.72%), Query Frame = 1 
Query:   94 NSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS----DKQLCNGPVKLCQSLNLTKE-FNQKNICNLNNLWIEDCNDTF--DVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
             S++ + +   + + ++   L+L   F++     LAK+LLGK+LVR  +    + GKI+ETESY G+ D  SHSYKG+ T RN+AMF+  GT Y Y IYG+ +C NI+ +E G+AVLIR+LEP++G+  M ++R +K   S    DK LCNGP KLCQ+  + K   N+ ++C  ++LW+E        D+V   RIGV+ YG++ +N   RYYIKGN YVS  NK
Sbjct:   62 QSVVQLSTYHSEEIILVGSALRLTKDFYNQACTILAKDLLGKVLVRRLESGEVLRGKIVETESYLGVEDKTSHSYKGKRTERNEAMFLDPGTAYVYTIYGMYYCFNISSREEGSAVLIRALEPLDGVPTMREFRSVKRNNSSTLKDKDLCNGPSKLCQAFKIEKTLLNKADLCTSSDLWLETGFTVARSDIVACKRIGVDSYGDEWANKPLRYYIKGNLYVSIKNK 287
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Match: G3WA01 (N-methylpurine DNA glycosylase OS=Sarcophilus harrisii OX=9305 GN=MPG PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.063e-52
Identity = 90/209 (43.06%), Postives = 133/209 (63.64%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKG-ETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIR--------SDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCND---TFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L P FFD    +LA+  LG+ILVR   D + + G+++ETE+Y G  D A+HS  G +T RNK MFM  GT+Y Y IYG+  C+N++ +  G  +L+RSLEPVEG++ M   R+L+N           D++LCNGP KLCQ+L + K F+Q+++    ++W+E   +      VV A+RIG+N  GE A   L R+YI+GN YVS +++
Sbjct:   68 RLGPEFFDQPAIALAQAFLGQILVRRLPDGTELRGRVVETEAYLGAEDEAAHSRGGRQTPRNKGMFMKPGTLYVYIIYGMYFCMNVSSQGEGACILLRSLEPVEGLEMM---RQLRNAHRKGAARALKDRELCNGPSKLCQALAIDKSFDQRDLAEEESIWLEQGPEKPGEHTVVTATRIGINYAGEWALKPL-RFYIRGNPYVSVVDR 272
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Match: A0A1W0WEV4 (Putative DNA-3-methyladenine glycosylase OS=Hypsibius dujardini OX=232323 GN=BV898_12106 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.902e-52
Identity = 99/232 (42.67%), Postives = 141/232 (60.78%), Query Frame = 1 
Query:   79 RRKVDNSIISMKSMKFQS--VDII-DPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDKQLCNGPVKLCQSLNLTK-EFNQKNICNLNNLWIEDCN---DTFDVVNASRIGVNGYGEKASNCL--YRYYIKGNSYVSKINK 747
            R KV+ ++    S  +QS  V+I   P + L   F++  V SLAK LLGK++VR   D  R+ G+I+ETE+Y G  DPASH+Y+G+T RN AMF  +GT Y Y IYG+ HC+NI+  E G  VLIR+L+P+ GI+ M  +RK         LCNGP +LCQ+ ++TK  F++ ++     LW+ED +     F +V + RIG+N     A +    +RYYI G   VSK +K
Sbjct:   21 RLKVEEAVREEVSAVYQSSPVEIARSPSVVLGSDFYNQTVHSLAKALLGKVVVREVKDGVRLKGRIVETEAYPGSTDPASHTYRGKTERNAAMFERAGTAYVYIIYGMYHCLNISSPEEGAGVLIRALQPLTGIETMRIFRKGGATLKQTDLCNGPGRLCQAFSITKTTFDKHDLHAGEALWLEDDSAGPSEFTIVESPRIGINPKSHDAESVRRPWRYYIMGCPDVSKKDK 252
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Cavefish
Match: mpg (N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZDB-GENE-070410-121])

HSP 1 Score: 167.162 bits (422), Expect = 4.398e-50
Identity = 86/212 (40.57%), Postives = 134/212 (63.21%), Query Frame = 1 
Query:  145 DPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS-----DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCN-----DTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +P  +L  +FF+     LA+  LGK+LVR   D + + G+++ETE+Y G  D ASHS  G+ T RN AMFM  GT+Y Y IYG+  C+N++ +  G AVL+R+LEP++G + M K R+ K         D++LCNGP KLCQ+L++ + F+++++     +W+ED        T  VV+A RIG++ +GE A+  L R+Y+  N+ VS +N+
Sbjct:   63 EPGGRLGFTFFNQPCVDLARAFLGKVLVRTQADGTELRGRVVETEAYLGGEDRASHSAGGKRTERNVAMFMKPGTIYVYPIYGIYLCMNVSSQGEGAAVLLRALEPLQGQNVMRKLREAKRREGAKTLRDRELCNGPSKLCQALDIQRRFDRRDLATDAQVWLEDSETPGSPGTGVVVSAPRIGIDSHGEWATKPL-RFYLHRNTCVSVVNR 273
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Cavefish
Match: mpg (N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZDB-GENE-070410-121])

HSP 1 Score: 167.548 bits (423), Expect = 8.135e-50
Identity = 86/212 (40.57%), Postives = 134/212 (63.21%), Query Frame = 1 
Query:  145 DPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS-----DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCN-----DTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +P  +L  +FF+     LA+  LGK+LVR   D + + G+++ETE+Y G  D ASHS  G+ T RN AMFM  GT+Y Y IYG+  C+N++ +  G AVL+R+LEP++G + M K R+ K         D++LCNGP KLCQ+L++ + F+++++     +W+ED        T  VV+A RIG++ +GE A+  L R+Y+  N+ VS +N+
Sbjct:   63 EPGGRLGFTFFNQPCVDLARAFLGKVLVRTQADGTELRGRVVETEAYLGGEDRASHSAGGKRTERNVAMFMKPGTIYVYPIYGIYLCMNVSSQGEGAAVLLRALEPLQGQNVMRKLREAKRREGAKTLRDRELCNGPSKLCQALDIQRRFDRRDLATDAQVWLEDSETPGSPGTGVVVSAPRIGIDSHGEWATKPL-RFYLHRNTCVSVVNR 273
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Sea Lamprey
Match: mpg (N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZDB-GENE-070410-121])

HSP 1 Score: 138.658 bits (348), Expect = 1.974e-40
Identity = 78/212 (36.79%), Postives = 124/212 (58.49%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVR-INDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS---------DKQLCNGPVKLCQSLNLTKEFNQKNICNLNN-LWIEDCNDTF---DVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L  SFF+     LA+ +LG+ LVR +      +  +++E+E+Y G  D ASHS  G  T RN AMFM  GT+Y Y IYGL+ C+N++ +  G+AVL+R+L+P+EG+  M   R+     S         D++LCNGP KLCQ+L+++   ++ +  + ++ LW E          +V A R+G+   G+ A    +R+YI+GN  VS ++K
Sbjct:   23 RLGFSFFNQPCEQLARAMLGQTLVRRVPGVEGALRARVVESEAYPGGLDEASHSRHGRRTPRNVAMFMPPGTLYVYRIYGLHCCLNVSSRGEGSAVLLRALQPLEGVATMRLLRQKPQQASTKCGRGQLPDRKLCNGPGKLCQALSISCALDRTDAASEDSELWFERGEGDVGEESMVAARRVGITSAGDWAER-PWRFYIRGNECVSVVDK 233
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Nematostella
Match: EDO48423 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJS4])

HSP 1 Score: 174.866 bits (442), Expect = 1.441e-54
Identity = 93/208 (44.71%), Postives = 133/208 (63.94%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDK-------QLCNGPVKLCQSLNLTKE-FNQKNICNLNNLWIE--DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L   F+D+    LAK+LLGK+L R+      + G+I+ETE+Y G  D A HS+ G+ T+RN+AMFM  GT Y Y+IYG+ +C+NI+ +E G  VL+R+LEPV+G+D M K+R  K+   DK       QLCNGP KLCQ+L++ K   N+K++   N  WIE  D    F VV + RIG++ YG +AS+   R+Y+ G   VS  +K
Sbjct:    4 RLGRLFYDIPTIDLAKDLLGKLLYRVLTGGEVVIGRIVETEAYLGRTDKACHSFGGKRTVRNEAMFMEPGTAYVYFIYGMYYCLNISSREEGACVLVRALEPVKGLDTMRKFRGAKH--KDKGAGLKEIQLCNGPSKLCQALDINKTNLNKKDLVTSNRFWIEEDDSGGRFKVVTSHRIGIDSYGAEASSQPLRFYVYGCRSVSVRDK 209
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Nematostella
Match: EDO48421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJS2])

HSP 1 Score: 170.629 bits (431), Expect = 6.707e-53
Identity = 91/203 (44.83%), Postives = 130/203 (64.04%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDK-------QLCNGPVKLCQSLNLTKE-FNQKNICNLNNLWIE-DCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVS 735
            +L   F+D+    LAK+LLGK+L R+      + G I+ETE+Y G  D A HS+ G+ T+RN+AMFM  GT Y Y+IYG+ +C+NI+ +E G  VLIR+LEPV+G+D M K+R  K+   DK       QLCNGP KLCQ+L++ K   N++++   N  WIE D +  F VV + R G++ YG +AS+   R+Y+ G   VS
Sbjct:    5 RLGRLFYDIPTIDLAKDLLGKLLYRVLTGGEVVIGLIVETEAYLGRTDKACHSFGGKRTVRNEAMFMAPGTAYVYFIYGMYYCLNISSREEGACVLIRALEPVKGLDTMRKFRGAKH--KDKGAGLKEIQLCNGPSKLCQALDINKTNLNKEDLVTSNRFWIEGDDSGRFKVVTSHRTGIDSYGAEASSQPLRFYVYGCRSVS 205
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Medaka
Match: mpg (N-methylpurine DNA glycosylase [Source:NCBI gene;Acc:101158026])

HSP 1 Score: 167.162 bits (422), Expect = 6.198e-50
Identity = 92/208 (44.23%), Postives = 131/208 (62.98%), Query Frame = 1 
Query:  157 KLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGE-TIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRS-----DKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCND-----TFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            +L   FF      LAK LLGK+LVR   D + + G+I+ETESY G  D ASHS  G+ T RN AMFM  GT+Y Y IYG+  C+NI+ +  G AVL+RSLEP++G+  M + R  +   S     DK+LCNGP KLCQ+LN+ + F+++++ +   +W+E   D       D+V+A RIG+  +GE A   L R+Y++G+  VS +NK
Sbjct:   67 RLGEDFFQQPCIRLAKALLGKVLVRRRSDGTELRGRIVETESYLGGEDKASHSAGGKRTARNLAMFMKPGTIYVYPIYGIYLCMNISSEGEGAAVLLRSLEPLQGLPIMRELRAARRRESARQLKDKELCNGPSKLCQALNIPRCFDRRDLASDPEVWLERDPDESPINPQDIVSAPRIGIESHGEWAKKPL-RFYLRGHPCVSVVNK 273
[Close]
BLAST of N-methylpurine-DNA glycosylase vs. Planmine SMEST
Match: SMESG000067444.1 (SMESG000067444.1)

HSP 1 Score: 454.521 bits (1168), Expect = 2.677e-164
Identity = 224/225 (99.56%), Postives = 224/225 (99.56%), Query Frame = 1 
Query:   73 MKRRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 747
            MKRRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGETIRNKAMFMGSGTVY YYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK
Sbjct:    1 MKRRKVDNSIISMKSMKFQSVDIIDPPLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLNDPASHSYKGETIRNKAMFMGSGTVYVYYIYGLNHCINITGKEIGTAVLIRSLEPVEGIDKMIKYRKLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNLNNLWIEDCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK 225
The following BLAST results are available for this feature:
BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
MPG3.656e-4236.54N-methylpurine DNA glycosylase [Source:HGNC Symbol... [more]
MPG4.608e-4236.54N-methylpurine DNA glycosylase [Source:HGNC Symbol... [more]
MPG5.152e-4236.54N-methylpurine DNA glycosylase [Source:HGNC Symbol... [more]
MPG3.771e-4040.37N-methylpurine DNA glycosylase [Source:HGNC Symbol... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mpg1.167e-4944.50N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZD... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mpg3.923e-5240.91N-methylpurine DNA glycosylase [Source:NCBI gene;A... [more]
mpg4.683e-5240.91N-methylpurine DNA glycosylase [Source:NCBI gene;A... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mpg4.631e-5139.81N-methylpurine-DNA glycosylase [Source:MGI Symbol;... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q04841|3MG_MOUSE3.239e-5039.81DNA-3-methyladenine glycosylase OS=Mus musculus OX... [more]
sp|P23571|3MG_RAT3.424e-4535.04DNA-3-methyladenine glycosylase (Fragment) OS=Ratt... [more]
sp|Q0SPY0|3MGH_CLOPS2.808e-4441.75Putative 3-methyladenine DNA glycosylase OS=Clostr... [more]
sp|A0PZK9|3MGH_CLONN3.929e-4442.08Putative 3-methyladenine DNA glycosylase OS=Clostr... [more]
sp|Q97EY6|3MGH_CLOAB3.813e-4340.87Putative 3-methyladenine DNA glycosylase OS=Clostr... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K7J9C95.500e-5539.75Uncharacterized protein OS=Nasonia vitripennis OX=... [more]
T1JKX31.391e-5448.51Uncharacterized protein OS=Strigamia maritima OX=1... [more]
A0A087SV846.123e-5443.81DNA-3-methyladenine glycosylase (Fragment) OS=Steg... [more]
G3WA012.063e-5243.06N-methylpurine DNA glycosylase OS=Sarcophilus harr... [more]
A0A1W0WEV43.902e-5242.67Putative DNA-3-methyladenine glycosylase OS=Hypsib... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mpg4.398e-5040.57N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZD... [more]
mpg8.135e-5040.57N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZD... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mpg1.974e-4036.79N-methylpurine DNA glycosylase [Source:ZFIN;Acc:ZD... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO484231.441e-5444.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO484216.707e-5344.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mpg6.198e-5044.23N-methylpurine DNA glycosylase [Source:NCBI gene;A... [more]
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BLAST of N-methylpurine-DNA glycosylase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000067444.12.677e-16499.56SMESG000067444.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005798 ID=SMED30005798|Name=N-methylpurine-DNA glycosylase|organism=Schmidtea mediterranea sexual|type=transcript|length=797bp
TTTATTAAATTACATATTTTAGTTACATTCAATTTAATTATTTATAACAT
TTCATGTAGTTTTCTTGTTAAAATGAAAAGAAGGAAAGTTGATAATTCCA
TTATTTCTATGAAATCAATGAAATTTCAATCTGTTGATATAATAGATCCA
CCATTAAAATTAAATCCCTCATTTTTCGATGTTGATGTGTTTAGTCTTGC
TAAAAATCTTTTGGGAAAAATTCTTGTAAGAATTAATGATGATAATTCTA
GAATCTCTGGTAAAATAATTGAAACAGAAAGTTATGATGGGTTGAATGAT
CCAGCTTCTCATTCTTATAAAGGTGAAACTATAAGAAATAAAGCCATGTT
TATGGGTAGTGGTACCGTTTATGATTATTATATTTACGGCCTCAATCATT
GCATCAATATTACTGGTAAAGAAATAGGTACTGCTGTACTTATTAGATCT
CTTGAGCCGGTTGAAGGTATTGATAAAATGATTAAATATCGCAAATTAAA
AAATATACGTTCTGATAAGCAATTATGTAATGGGCCTGTTAAATTATGTC
AATCTTTGAATTTAACAAAGGAATTTAATCAAAAAAATATTTGCAATTTG
AACAATTTATGGATTGAAGATTGTAATGATACTTTTGATGTGGTAAATGC
TTCGAGAATTGGAGTAAATGGTTACGGTGAAAAGGCTTCAAATTGCTTGT
ATAGATATTATATTAAGGGCAATTCTTATGTCAGTAAAATAAATAAATAA
CTTTGTATTTTTTGTGAAAAATAAATGTTTCTTTATCTTAAAAAAAA
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protein sequence of SMED30005798-orf-1

>SMED30005798-orf-1 ID=SMED30005798-orf-1|Name=SMED30005798-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
FIKLHILVTFNLIIYNISCSFLVKMKRRKVDNSIISMKSMKFQSVDIIDP
PLKLNPSFFDVDVFSLAKNLLGKILVRINDDNSRISGKIIETESYDGLND
PASHSYKGETIRNKAMFMGSGTVYDYYIYGLNHCINITGKEIGTAVLIRS
LEPVEGIDKMIKYRKLKNIRSDKQLCNGPVKLCQSLNLTKEFNQKNICNL
NNLWIEDCNDTFDVVNASRIGVNGYGEKASNCLYRYYIKGNSYVSKINK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036995MPG_sf
IPR003180MPG
IPR011034Formyl_transferase-like_C_sf
Vocabulary: molecular function
TermDefinition
GO:0003905alkylbase DNA N-glycosylase activity
GO:0003677DNA binding
GO:0003824catalytic activity
Vocabulary: biological process
TermDefinition
GO:0006284base-excision repair
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036995Methylpurine-DNA glycosylase superfamilyGENE3DG3DSA:3.10.300.10coord: 50..249
e-value: 3.9E-67
score: 227.6
IPR003180Methylpurine-DNA glycosylasePFAMPF02245Pur_DNA_glycocoord: 57..241
e-value: 5.4E-54
score: 182.3
IPR003180Methylpurine-DNA glycosylaseTIGRFAMTIGR00567TIGR00567coord: 54..245
e-value: 3.4E-54
score: 181.3
IPR003180Methylpurine-DNA glycosylasePANTHERPTHR10429DNA-3-METHYLADENINE GLYCOSYLASEcoord: 36..249
IPR003180Methylpurine-DNA glycosylaseCDDcd00540AAGcoord: 58..245
e-value: 1.23633E-81
score: 240.889
IPR011034Formyl transferase-like, C-terminal domain superfamilySUPERFAMILYSSF50486FMT C-terminal domain-likecoord: 53..249
NoneNo IPR availableTMHMMTMhelixcoord: 4..23