SMED30005785

Overview
NameSMED30005785
Smed IDSMED30005785
Length (bp)2399
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005785 (SMED30005785) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005785 (SMED30005785) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005785 (SMED30005785) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005785 (SMED30005785) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005785

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30005785SMESG000008183.1 dd_Smed_v4_13129_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005785SMESG000008183.1 dd_Smed_v4_13129_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30005785SMESG000008183.1 dd_Smed_v4_13129_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30005785SMESG000008183.1 dd_Smed_v6_13129_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
enteric muscle cellSMED30005785SMESG000008183.1 dd_Smed_v4_13129_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30005785 vs. TrEMBL
Match: A0A4S2MB77 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001327 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.748e-8
Identity = 112/461 (24.30%), Postives = 180/461 (39.05%), Query Frame = 1 
Query:  619 LDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFT-------------SGIGETMDIINWKRQQINVTNIILGF----MRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFK--LNIHVSRAYNTD---KYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPK-IENFIFLCSNLNSFNVNLD----------KRYQESKIFINAFAINSKTKFTDSY------RPVEYRWGQFEHRCQTKKKKNV-KIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQE-------QSLHVFQKESQTPLCVE-----IVHSRLTAFCT-SLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTY 1842
            L I EK L +    P+  + ++   + P       + Y V      W+ +TPC+  N    +Q F +    R   KT  R     +S        N   T             S   +  +I+N +R +I+ T+         ++LY    D       IR+        YP +       +C+ D     L I  + A + D   +YCVS+N +E L   CT   R   S+L+Q   Y  L   RP  F      R  P+ I NF++ C    +  V L            +   +K+FIN +A+N++   + +Y      RPVE        RC + ++ +V K++ L S   I     + F +   G+   + FYQ+C    +         L+  ++ S TP  +E     I+ SR+ A C+ +   G Y LQ    A    +   G+ Y    NTY
Sbjct:   99 LPIGEKILEKGRLMPQGFDAIEFPVVIPKGRFVKTLRY-VIAKPRFWLTITPCT--NRLDRIQ-FTTTRASRVNLKTNSRYALVLNSTSSARRRYNQSITVERFPGSPQLPNVSISDDEREILNLERSRIHYTSRRYSGSALEVQLYGRPGDAFLVRMSIRESPTHTFTGYPELSNPQLHSVCLVDGTSDTLKIRWAAALSDDMQPQYCVSINAEENLYYQCTAYARLNASILRQ---YGTLD--RPASFGKTEQLR--PRTINNFVYTCLPNKTLQVRLPPALALSLPRLGKDASAKLFINVYAVNTRVSLSTAYAVRVLDRPVE--------RCGSVRQVDVMKVHSLPSFHKIRWATDIEFELP-AGVRQFEAFYQTCKIPGDFTAKLISVQLYHIRETSSTPQRIEVCRQPIIDSRIHAICSQNHTGGYYLLQVKGPAGINHSQESGRFYLRQPNTY 539
[Close]
BLAST of SMED30005785 vs. TrEMBL
Match: A0A4E0R7J1 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_007162 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.786e-8
Identity = 104/480 (21.67%), Postives = 183/480 (38.12%), Query Frame = 1 
Query:  622 DITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCST-------SNYTI----------------TVQSFQSDETIR---------------PPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYS-----GKIDAFYPRIPPYSTVEICVTDNFK--LNIHVSRAYNTD---KYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDY----------YLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKI-YRLKSHL-WIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQ----SLHVFQKE------SQTPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYP 1851
             + E  L Q +  PR  + ++   +     +PL + Y +  ++  WI +TPC+        S   I                + +S +  ET R               PPN T   D+ E  ++Y       + ++          N+ R  +N+        RL+    D    AY++R  +      K+D  Y + P  ST+ +CV       L +  + A   D    YCV++N  E LP  C        S L ++    D+   RP  F +            +Y  +   E  I L + L     +        K+++N + IN +T+ + SY P   +     H CQ  ++ + +I  R+ S   ++  N   +F      + P +LF+Q C   +      ++H+++ +       +    + I  SR+T     L PG YRL     A+ +S   V + +FL E +   P
Sbjct:   97 QLGEDRLKQGSVIPRIFDAIEFAAVQER-SDPLMLQYSLGNNSSPWITITPCTGPLDGIKFSQSAIYHKKWKESLRFAFQLASSKSGERKETSRKAGGIWIEPFPGEEHPPNHTLSVDETELSNFYQS----RVHYSRS--------NYSRDTMNI--------RLFGRTGD----AYMVRVSTRSSPFHKLDG-YLKPPKSSTISVCVQSRSSDTLEVRWNPAVTEDVRPTYCVAVNTQENLPYRC--------SALARLNPVNDVQFHRPRWFRETDHLRSDLIKPSVYHCVNSTEAQIKLPTQLAQLVTSPPTNSSPVKLYVNVYVINIRTELSASYEPSIQQLPL--HTCQFIRRTSSRIPVRVNSSPNYLAWNAEAIFKWP-TSIPPAQLFFQPCQLGKSGQDHYTVHLYEMQQAHRHLERELFQININKSRITKVTNQLHPGSYRLYMQGPARFKSGEHVARFFFLTEQSNLLP 539
[Close]
BLAST of SMED30005785 vs. TrEMBL
Match: A0A3R7F1K0 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_9582s PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.393e-7
Identity = 111/465 (23.87%), Postives = 177/465 (38.06%), Query Frame = 1 
Query:  619 LDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFT-------------SGIGETMDIINWKRQQINVTNIILGF----MRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFK--LNIHVSRAYNTD---KYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPK-IENFIFLCSNLNSFNVNLD----------KRYQESKIFINAFAINSKTKFTDSY------RPVEYRWGQFEHRCQTKKKKNV-KIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSC-------MHSQEQSLHVFQKESQTPLCVE-----IVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQ----LYFLPENTYQ 1845
            L I EK L +    P+  + ++   + P       + Y V      W+ +TPC+  N    +Q F +    R   KT  R     +S        N   T             S   +  +I+N +R +I+ T+         ++LY    D       IR+        YP +       +C+ D     L I  + A + D   +YCVS+N +E L   CT   R   ++L+Q        I RP  F      R  P+ I NF++ C    +  V L            +   +K+FIN +A+N++   + +Y      RPVE        RC + +  +V K++ L S   I     + F +   G+   + FYQ+C         S    L+  ++   T   +E     I+ SR+ A CT    G Y L   L  K  + +  GQ     Y    NTY+
Sbjct:   99 LPIGEKILEKGRLMPQGFDAIEFPVVIPKGRFVKTLRY-VIAKPRFWLTITPCT--NRLDRIQ-FTTTRASRVNLKTNNRYALVLNSTSSARRRYNQSITVERFPGNPQLPNVSISDDEREILNLERSRIHYTSRRYSGSALEVQLYGRPGDAFLVRMSIRESPTHTFTGYPELSDSQIHSVCLVDGTSDTLKIRWAAALSDDMQPQYCVSINAEENLYYQCTAYARLNATILRQYGT-----IDRPASFGKTEQLR--PRTINNFVYTCLPNKTLQVRLPPALALSLSRLGKDTSAKLFINVYAVNTRVSLSTAYAVRVLDRPVE--------RCGSVRPVDVMKVHSLPSFHKIRWATDIEFELP-AGVRQFEAFYQTCKVPGDFTAKSISVQLYHIRETPGTAQRIEVCRQPIIDSRIHAVCTQNHTGGYYL---LQVKEPAGINHGQESGRFYLRQPNTYE 540
[Close]
BLAST of SMED30005785 vs. TrEMBL
Match: A0A1S8X8U7 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_00928 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.270e-6
Identity = 81/337 (24.04%), Postives = 139/337 (41.25%), Query Frame = 1 
Query:  940 DIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAF--YPRIPPYSTVEICVTDNFK--LNIHVSRAYNTD---KYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPK-IENFIFLCSNLNSFNVNLD----------KRYQESKIFINAFAINSKTKFTDSY------RPVEYRWGQFEHRCQTKKKKNV-KIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSC-----MHSQEQSLHVFQKESQTPLCVE-----IVHSRLTAFCT-SLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTY 1842
            +I+N +R +I+ T+       L +        A+++R        F  YP +       +C+ D     L I  + A + D   +YCVS+N +E L   CT   R   ++L+Q        + RP  F      R  P+ I NF++ C    +  V L            +   +K+F+N +A+N++   + +Y      RPVE        RC + +  +V K++ L S   I     + F +  + +   + FYQ+C       ++  S+ ++      P  +E     IV SR+ A C+ +   G Y LQ    A    +   G+ Y    NTY
Sbjct:   30 EILNLERSRIHYTSRRYSGSALEVQLYGRAGDAFLVRMRESPTHTFTGYPELSNPQIHSVCLVDGTSDTLEIRWAAALSDDMQPQYCVSINAEENLYYQCTAYARLNATILRQYGT-----MDRPASFGKTEQLR--PRTINNFVYTCLPNKTLQVRLPHTLALNLSRLGKDASAKLFVNVYAVNTRVSLSTAYAVRVLDRPVE--------RCGSVRPVDVMKVHSLPSFHKIRWATDIEFELPAS-VRQFEAFYQTCKIPGDFTAKPISVQLYHIRETPPQRIEVCRQPIVDSRIHAICSQNHTGGYYLLQVKEPAGINHSQESGRFYLRQPNTY 350
[Close]
BLAST of SMED30005785 vs. TrEMBL
Match: A0A5K4F2Y6 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.382e-6
Identity = 86/320 (26.88%), Postives = 137/320 (42.81%), Query Frame = 1 
Query:  997 MRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDN--------FKLNIHVSRAYNTDKYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDY-QDLHIRRPEVFNDY-YLYRKIPKIENFIFLCSNLNSFNVNLDKRYQES----------KIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKN--VKIYRL--KSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQ----SLHVFQKESQ----------TPLCVEIVHS-RLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENT 1839
            +RLY    DT    +  R+        YP +   ST+ +C+  N        +K  IH S   N   YCV +N DE L   CT   R     L Q+ +Y  +    RP++F D  YL  K+  I+N+++ CS  +   + L     +           K++IN +AIN +   + SY PV+      E  CQ+    N  + I +L      W   N  +LF ++  G  P +L+YQ C     +    ++ + +K +             LC   ++S R+   C +   G+Y LQ   N   ++  PVG+ +FL  NT
Sbjct:   29 VRLYGNIGDTYNIRFSTRRSPKHALDGYPELDQTSTISVCLVQNTNDTLDISWKGVIHNSMQIN---YCVVINADENLYYKCTAMSR-----LNQLFNYYTNEKFERPKIFQDTDYLRPKL--IDNYVYKCSTKSMIRIKLTPFLSQIINHLPSNNSLKLYINIYAINKRMDISTSY-PVKIIQYPIE-LCQSNYHYNNLIIINKLYYNQFYW---NSNILFQLQFTG-NPLQLYYQPCYLPSNKLFNYTISLLKKLTSKNSTMTTSNLQTLCEYPINSHRVLRMCLNSGNGIYYLQLNGNPNFKNRQPVGRYFFLGINT 332
[Close]
BLAST of SMED30005785 vs. Planmine SMEST
Match: SMESG000008183.1 (SMESG000008183.1)

HSP 1 Score: 1315.83 bits (3404), Expect = 0.000e+0
Identity = 633/635 (99.69%), Postives = 633/635 (99.69%), Query Frame = 1 
Query:  418 MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQSLHVFQKESQTPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDSRFNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF 2322
            MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDE LPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQSLHVFQKESQTPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDS FNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF
Sbjct:    1 MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDEILPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQSLHVFQKESQTPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDSLFNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF 635
[Close]
BLAST of SMED30005785 vs. Planmine SMEST
Match: SMESG000008183.1 (SMESG000008183.1)

HSP 1 Score: 1263.83 bits (3269), Expect = 0.000e+0
Identity = 613/635 (96.54%), Postives = 615/635 (96.85%), Query Frame = 1 
Query:  418 MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNKLFYQSCMHSQEQSLHVFQKESQTPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDSRFNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF 2322
            MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDE LPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNK                 +K S+TPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDS FNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF
Sbjct:    1 MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIFICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNPLAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKKEFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAKSDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNTDKYCVSLNLDEILPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYLYRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSYRPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYPNK-----------------KKASETPLCVEIVHSRLTAFCTSLKPGMYRLQHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDSKTFYRLNITFSRVPSLSDITYYFHIINFKIPNDSLFNQIEFMSQIEAKIKKERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNTYAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF 618
The following BLAST results are available for this feature:
BLAST of SMED30005785 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2MB771.748e-824.30Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4E0R7J13.786e-821.67Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A3R7F1K02.393e-723.87Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A1S8X8U71.270e-624.04Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A5K4F2Y62.382e-626.88Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of SMED30005785 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005785 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008183.10.000e+099.69SMESG000008183.1[more]
SMESG000008183.10.000e+096.54SMESG000008183.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005785 ID=SMED30005785|Name=SMED30005785|organism=Schmidtea mediterranea sexual|type=transcript|length=2399bp
AAAAGTTTTCAACAAAAAGGTAAAAAATTATTGGACGAGAGTTTCGTTGT
TTTTGTGACCTGAATAGAGTCTAGACACAATTCTCAATTCGGACAATGCA
CAATATCACTGTGCGTAAGACCGGGCTAATCGGGGCCCGGGATCCCACTT
TAAGAAATTCGATGTGCCATAGAAATTATGTTGAGAAATCTGCACAAACA
AGGGGTTGATGGATTGTAATAGATCGATAATTTCCTTCCTCTGACAGCAG
TGTTGACTAACTGCAGCTTCCCAGTTTGCGACTGCACTGCAGTTACAACA
GACGATGCGACCAATCACATTGTCGACAGAACTGACTGGTACTGTTTGTT
TTAATAGAATTTGACAATATTTATCCGATGACTCTCGACCACCTTTCATT
TCTGCTCACGAATCAAGATGCATCATAACTCTATCCGACATTCAGATCAC
CATTGGGGTGGTTACTCACTGCTCAGCCCTCACCGGATCCGACCCCCCGA
TATATTCTTTATCGGAACATATCCTCGATATGGAGTATGGCTATCATTTT
TGTGCCTGTTGATTTTTATATGTCCTCGAGTTGGATCACAATTTCGACAG
ATCATTAATAATTATGACCTCGATATTACTGAGAAATTTCTTTCACAGAA
AACGAAATTTCCCCGAAATTTAGAAACTCTTGATTTGGGATTCATTAATC
CTTCCCCTTACAATCCTTTAGCAGTTTACTATGATGTATATCCCCACAAC
GAATCTTGGATATTTGTCACACCTTGTAGCACCTCGAATTACACAATTAC
TGTCCAATCTTTCCAATCAGATGAGACAATCAGACCTCCGAACAAGACAA
AAAAGCGTGACAAAAAAGAATTTGACAGTTGGTATGATAAAGTAACAGTA
ATAAACATGAAATTTACTTCGGGAATCGGAGAAACGATGGACATAATTAA
CTGGAAACGACAGCAGATAAATGTCACAAATATTATTTTGGGGTTTATGA
GGTTGTATATCGCTAAATCAGATACTCTTCCCGCTGCCTATGTGATCAGA
CAATATTCTGGTAAAATAGATGCTTTTTATCCAAGAATTCCACCATATTC
AACAGTAGAGATTTGCGTAACGGATAATTTCAAGCTCAACATTCACGTTT
CACGTGCATACAATACGGACAAATACTGTGTCAGTTTGAATCTCGATGAA
ACTCTACCAAATTTCTGCACGGTTCAATTGCGCTGTAAGAAATCCTTGCT
AAAACAAATCAGAGATTACCAAGACCTTCACATAAGACGCCCAGAGGTAT
TTAATGATTATTATCTATACAGAAAAATTCCAAAAATTGAAAACTTTATA
TTCCTTTGTTCGAATCTAAACAGTTTCAATGTCAATCTAGACAAACGTTA
TCAAGAGTCAAAAATCTTCATCAATGCCTTTGCAATAAACAGCAAAACAA
AATTCACAGATTCATACCGACCAGTTGAATATCGGTGGGGACAGTTTGAA
CACCGTTGCCAAACTAAAAAGAAGAAAAATGTTAAAATCTACAGATTGAA
GTCACATTTATGGATAATGGGAAATGAAACTGTCCTTTTTAATATTGAAC
GTAACGGACTGTATCCAAACAAGTTATTTTATCAGAGTTGTATGCATTCC
CAAGAGCAATCATTGCACGTATTCCAGAAAGAAAGCCAGACACCCCTTTG
TGTCGAAATTGTACATAGTCGATTGACAGCGTTTTGTACCTCACTGAAGC
CGGGAATGTATCGTCTACAGCATACATTAAACGCAAAGTCAAGATCAACA
GTCCCGGTAGGACAACTCTACTTCCTCCCAGAAAACACTTATCAATATCC
GCATTTACATTGCAATAGGACAACAGATTTCTCAATCAACTTGCATAAAG
CCGCGTTAGATGATTCGAAAACCTTTTACAGACTTAACATAACATTCAGT
CGAGTTCCCAGCTTATCAGACATAACGTACTACTTTCACATTATCAATTT
CAAAATTCCAAACGACAGCCGGTTTAATCAAATTGAATTTATGTCTCAAA
TAGAGGCAAAAATTAAAAAAGAGAGAAACTATTGCAAAATCGATGATAAA
AAATTAGGAGGGGAAAGAATCGTCCACAGTGTGACTTTGTCAGGACTTGG
GTGTTCAGATTCAATTCAGAGAGAAGATTACGCAGCCATTCCCGGATTAT
TCAATAGGAACAATACGTATGCCGTTTTAGTTTATTTTACCAAATCAACT
GACAAGAGGCTGAATTACGTTCAATTTTATTCCAATCGGATTTTTACTAT
GTCCTCAGAAGAGGATGTATTTTAGGAGAGATTTTCTATTTAATTAACTC
GGTCTTACCTCATCAATTAACGCATTTTACGATGTTTATTAAATAAAGA
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protein sequence of SMED30005785-orf-1

>SMED30005785-orf-1 ID=SMED30005785-orf-1|Name=SMED30005785-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=636bp
MHHNSIRHSDHHWGGYSLLSPHRIRPPDIFFIGTYPRYGVWLSFLCLLIF
ICPRVGSQFRQIINNYDLDITEKFLSQKTKFPRNLETLDLGFINPSPYNP
LAVYYDVYPHNESWIFVTPCSTSNYTITVQSFQSDETIRPPNKTKKRDKK
EFDSWYDKVTVINMKFTSGIGETMDIINWKRQQINVTNIILGFMRLYIAK
SDTLPAAYVIRQYSGKIDAFYPRIPPYSTVEICVTDNFKLNIHVSRAYNT
DKYCVSLNLDETLPNFCTVQLRCKKSLLKQIRDYQDLHIRRPEVFNDYYL
YRKIPKIENFIFLCSNLNSFNVNLDKRYQESKIFINAFAINSKTKFTDSY
RPVEYRWGQFEHRCQTKKKKNVKIYRLKSHLWIMGNETVLFNIERNGLYP
NKLFYQSCMHSQEQSLHVFQKESQTPLCVEIVHSRLTAFCTSLKPGMYRL
QHTLNAKSRSTVPVGQLYFLPENTYQYPHLHCNRTTDFSINLHKAALDDS
KTFYRLNITFSRVPSLSDITYYFHIINFKIPNDSRFNQIEFMSQIEAKIK
KERNYCKIDDKKLGGERIVHSVTLSGLGCSDSIQREDYAAIPGLFNRNNT
YAVLVYFTKSTDKRLNYVQFYSNRIFTMSSEEDVF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0003104enteric muscle cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 29..51