SMED30005771
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005771 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30005771 aligns in the following genomic locations:
Homology
BLAST of SMED30005771 vs. TrEMBL
Match: A0A1S3HM18 (uncharacterized protein LOC106155680 OS=Lingula unguis OX=7574 GN=LOC106155680 PE=4 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 2.755e-9 Identity = 62/202 (30.69%), Postives = 94/202 (46.53%), Query Frame = 2 Query: 332 GIITGLIIMSDYQRRTFADQYNKSYN-DISICYNEFPLYDRNVSISPWFVCP-WKDP-RYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNNPIIYTENLSNLPYPTYNDLSMEYQPEF---PPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPENP 919 G + GL S YQRR +Y + + + CYN + N S+ F+CP W P YCCG E+++ CC ++D G +AG++IG++I L+ I C + R SC K + T ++ + P S++P YQP+ PP YP++P TD QP+ + S E + P SP P Sbjct: 55 GTVYGL---SRYQRRR---RYKMDPDREPTRCYNSARRFQENGSLFNEFICPEWDQPDELEYCCGPELQQYCCGFWDDGGRVAGVVIGILIAVTLVIGCIACCCCQARKRRASC-----KSTTTGTTEMTSYP-------SSVP---------TYQPQMAQPPPAYPLNP----TDETSQPLMS-SSGEKDASGPGFSPYPP 224
BLAST of SMED30005771 vs. TrEMBL
Match: A0A1S3HKT4 (uncharacterized protein LOC106155682 OS=Lingula unguis OX=7574 GN=LOC106155682 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 1.742e-8 Identity = 30/83 (36.14%), Postives = 45/83 (54.22%), Query Frame = 2 Query: 422 CYNEFPLYDRNVSISPWFVCP-WKDP-RYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSC 664 CYN + N S+ F+CP W P YCCG E+++ CC ++D G +AG++IG++I L+ I C + R SC Sbjct: 10 CYNSARRFQENGSLFNEFICPEWDQPDELEYCCGPELQQYCCGFWDDGGRVAGVVIGILIAVTLVIGCIACCCCQARKRRASC 92
BLAST of SMED30005771 vs. TrEMBL
Match: V4CHQ0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_238206 PE=4 SV=1) HSP 1 Score: 65.855 bits (159), Expect = 2.161e-8 Identity = 55/196 (28.06%), Postives = 89/196 (45.41%), Query Frame = 2 Query: 416 SICYNEFPLYDRNVSISPWFVCP-WKDPR-YRYCCGDEIEERCCRYFDRPGHLAGLIIGVI----ILCALIYIVIK-YCHVMVKHCRRSCSKVVYKHDS----PKTAKLDNNP---IIYTENLSNLPY---PTYNDLSMEYQPEFPPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPEN--PPPSYSEIKN 946 +IC N F D N + +F+CP +P + YCCGD+ E CC Y+++ G + G++ GV+ +L L+Y IK Y V S + Y + + T ++NP +T ++S+ PY P D + Y + P Y ++P + + P P + F P + PPP YS+ N Sbjct: 108 TICTNNFDK-DSNGTTYGYFICPRAGEPEDHEYCCGDDKREHCCEYYNQTGRIVGIVFGVLCGVTVLSVLLYCCIKHYKKDKVDDKFNSIRQGSYNNGNTTTPASTGPYNSNPPTSTAFTTSVSDNPYDSKPISGDATPAYDSKSAPPYSLEPT---SAFTYSPTPAATAGTVPLGFEYSDPAHYTPPPPYSQAVN 299
BLAST of SMED30005771 vs. TrEMBL
Match: K1P0G4 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10002070 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 1.681e-7 Identity = 44/152 (28.95%), Postives = 78/152 (51.32%), Query Frame = 2 Query: 419 ICYNEFPLYDRN----VSISPWFVCPWKD---PRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNN-------PIIYT---------ENLSNLPYPTYNDLSMEYQPEFPP 805 +CYN+ Y+R+ VS F CP + YRYCCG+E ++ CC ++D+PGH+ G++ G+I +++V+ +C ++K+ + + S+ H+ P T+ + N P Y ++ PYPT +D+ P +PP Sbjct: 188 VCYND--SYNRSTNGTVSYQGRFFCPLDENMSEDYRYCCGEEGQQYCCTFWDKPGHIVGVVFGIIAGVVALFVVV-FC--VIKYMKTTKSR---NHNRPYTSNISNTSNGHASYPPAYAPPTYEMSCVDSNGKTPYPT-SDMMGYSNPSYPP 330
BLAST of SMED30005771 vs. TrEMBL
Match: K1PDZ5 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10007565 PE=4 SV=1) HSP 1 Score: 55.8398 bits (133), Expect = 7.607e-6 Identity = 25/75 (33.33%), Postives = 43/75 (57.33%), Query Frame = 2 Query: 446 DRNVSISPWFVCP---WKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRS 661 + VS F CP + YRYCCG+E ++ CC ++D+PGH+ G++ G+I ++I + +C V ++S Sbjct: 4 NDTVSYQGRFFCPIDEYMSEDYRYCCGEEGQQNCCTFWDKPGHIVGVVFGIIAGMVALFIAV-FCVVKCIRTKKS 77
BLAST of SMED30005771 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 464.537 bits (1194), Expect = 5.417e-166 Identity = 293/298 (98.32%), Postives = 296/298 (99.33%), Query Frame = 2 Query: 59 KASLIIGIIVITGFLVIISEEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYNKIKVSVRYXXXXXXXXXXXXXXXXXXXXXXXXTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNNPIIYTENLSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 952 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYI IKYCHVM+KHCRRSCSKVVYKHDSPK AKLDNNPIIYTEN+SNLPYPTYNDLSMEYQPEFPPQYPVDPQNPG+DYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE Sbjct: 5 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPKNAKLDNNPIIYTENVSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGSDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 302
BLAST of SMED30005771 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 464.151 bits (1193), Expect = 7.935e-166 Identity = 293/298 (98.32%), Postives = 296/298 (99.33%), Query Frame = 2 Query: 59 KASLIIGIIVITGFLVIISEEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYNKIKVSVRYXXXXXXXXXXXXXXXXXXXXXXXXTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNNPIIYTENLSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 952 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYI IKYCHVM+KHCRRSCSKVVYKHDSPK AKLDNNPIIYTEN+SNLPYPTYNDLSMEYQPEFPPQYPVDPQNPG+DYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE Sbjct: 8 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPKNAKLDNNPIIYTENVSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGSDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 305
BLAST of SMED30005771 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 434.491 bits (1116), Expect = 3.133e-154 Identity = 282/298 (94.63%), Postives = 285/298 (95.64%), Query Frame = 2 Query: 59 KASLIIGIIVITGFLVIISEEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYNKIKVSVRYXXXXXXXXXXXXXXXXXXXXXXXXTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNNPIIYTENLSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 952 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYI IKYCHVM+KHCRRSCSKVVYKHDSPK EN+SNLPYPTYNDLSMEYQPEFPPQYPVDPQNPG+DYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE Sbjct: 8 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPK-----------NENVSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGSDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 294
BLAST of SMED30005771 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 434.491 bits (1116), Expect = 3.390e-154 Identity = 282/298 (94.63%), Postives = 285/298 (95.64%), Query Frame = 2 Query: 59 KASLIIGIIVITGFLVIISEEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYNKIKVSVRYXXXXXXXXXXXXXXXXXXXXXXXXTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLDNNPIIYTENLSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGTDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 952 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYI IKYCHVM+KHCRRSCSKVVYKHDSPK EN+SNLPYPTYNDLSMEYQPEFPPQYPVDPQNPG+DYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE Sbjct: 5 KASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSSGFRSSVRSRGVSYNKIKVSVRYSSWSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPK-----------NENVSNLPYPTYNDLSMEYQPEFPPQYPVDPQNPGSDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEIKNYE 291
BLAST of SMED30005771 vs. Planmine SMEST
Match: SMESG000042005.1 (SMESG000042005.1) HSP 1 Score: 156.762 bits (395), Expect = 4.738e-45 Identity = 120/275 (43.64%), Postives = 157/275 (57.09%), Query Frame = 2 Query: 230 NKIKVSVRYXXXXXXXXXXXXXXXXXXXXXXXXTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIVIKYCHVMVKHCRRSCSKVVYKHDSPKTAKLD-NNPIIY-------TENLSNLPYPTYNDLS---MEYQPEFP-------------------------PQYPVDPQN--PGTDYVEQPMPTYVSDESNQHFPLLSPENPPPSYSEI 940 KI S RYS WSRSHG SSSFRYR+S+Y YKR GI +GLI++S R T+ + S ++ISICYNE PLY RN+S SPWFVCPW DPR+RYCCG+EIEERCCRYFDRPGHL GL+IG +++ I++++ C KH + + + + P +D N I+Y T+ + PYP DL + QP+F P YP+ P + P +Q + DES + L+ PENPPPSYS+I Sbjct: 51 GKIGSSSRYSMWSRSHGFSSSFRYRTSTYYYKRNGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVM-VFIFVLVCQCFCK-KHKHKEKADIALVNVDPDKIDIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPHSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDI 323 The following BLAST results are available for this feature:
BLAST of SMED30005771 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005771 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005771 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30005771 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005771 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005771 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005771 ID=SMED30005771|Name=SMED30005771|organism=Schmidtea mediterranea sexual|type=transcript|length=998bpback to top protein sequence of SMED30005771-orf-1 >SMED30005771-orf-1 ID=SMED30005771-orf-1|Name=SMED30005771-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=306bp MLLMQNFKASLIIGIIVITGFLVIISEEARSGGSRGGGSRSSRSGSSRSSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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