V-type proton ATPase subunit F

Overview
NameV-type proton ATPase subunit F
Smed IDSMED30005719
Length (bp)560
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of V-type proton ATPase subunit F (SMED30005719) t-SNE clustered cells

Violin plots show distribution of expression levels for V-type proton ATPase subunit F (SMED30005719) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of V-type proton ATPase subunit F (SMED30005719) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for V-type proton ATPase subunit F (SMED30005719) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005719

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30005719h1SMcG0010350 PL04025A1B03smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30005719h1SMcG0010350 dd_Smed_v4_1589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Human
Match: ATP6V1F (ATPase H+ transporting V1 subunit F [Source:HGNC Symbol;Acc:HGNC:16832])

HSP 1 Score: 170.244 bits (430), Expect = 1.694e-54
Identity = 79/117 (67.52%), Postives = 95/117 (81.20%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F  F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RA+GMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Human
Match: ATP6V1F (ATPase H+ transporting V1 subunit F [Source:HGNC Symbol;Acc:HGNC:16832])

HSP 1 Score: 154.836 bits (390), Expect = 4.486e-48
Identity = 79/145 (54.48%), Postives = 96/145 (66.21%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHN----------------------------FVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F +                            F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RA+GMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRSLGSLPGSVVEANPNQRDPPLWDEIDSRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR 147
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Celegans
Match: vha-9 (Probable V-type proton ATPase subunit F [Source:UniProtKB/Swiss-Prot;Acc:Q23680])

HSP 1 Score: 165.236 bits (417), Expect = 7.565e-53
Identity = 77/119 (64.71%), Postives = 97/119 (81.51%), Query Frame = 1 
Query:   58 TLAKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            + AKGK++AVIGDEDT  GFLLGGVGEL+  RKPN+L+V+K T + +IE AF+ F  R DIAIILI Q+IAE IR+ +D H  ++PAVLEIPS++ PYDPSKDSIL RA+G+F+ EDFR
Sbjct:    3 SAAKGKILAVIGDEDTVVGFLLGGVGELNKARKPNYLIVDKQTTVQEIEEAFNGFCARDDIAIILINQHIAEMIRYAVDNHTQSIPAVLEIPSKEAPYDPSKDSILNRARGLFNPEDFR 121
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Celegans
Match: vha-9 (Probable V-type proton ATPase subunit F [Source:UniProtKB/Swiss-Prot;Acc:Q23680])

HSP 1 Score: 165.236 bits (417), Expect = 7.565e-53
Identity = 77/119 (64.71%), Postives = 97/119 (81.51%), Query Frame = 1 
Query:   58 TLAKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            + AKGK++AVIGDEDT  GFLLGGVGEL+  RKPN+L+V+K T + +IE AF+ F  R DIAIILI Q+IAE IR+ +D H  ++PAVLEIPS++ PYDPSKDSIL RA+G+F+ EDFR
Sbjct:    3 SAAKGKILAVIGDEDTVVGFLLGGVGELNKARKPNYLIVDKQTTVQEIEEAFNGFCARDDIAIILINQHIAEMIRYAVDNHTQSIPAVLEIPSKEAPYDPSKDSILNRARGLFNPEDFR 121
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Match: Vha14-1 (gene:FBgn0262512 transcript:FBtr0087341)

HSP 1 Score: 168.318 bits (425), Expect = 6.434e-54
Identity = 78/115 (67.83%), Postives = 96/115 (83.48%), Query Frame = 1 
Query:   67 KGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDF 411
            KGKLI+VIGDEDTC GFLLGGVGE++  R PNF+VV+K+T ++++E+ F  F+KR DI IILI QN AE IRH++D H S VPAVLEIPS+DHPYD SKDSIL+RA+GMF+ ED 
Sbjct:    8 KGKLISVIGDEDTCVGFLLGGVGEINKNRHPNFMVVDKNTAVSELEDCFKRFLKRDDIDIILINQNCAELIRHVIDAHTSPVPAVLEIPSKDHPYDASKDSILRRARGMFNPEDL 122
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Match: Vha14-2 (gene:FBgn0037402 transcript:FBtr0309511)

HSP 1 Score: 137.887 bits (346), Expect = 6.167e-42
Identity = 66/110 (60.00%), Postives = 83/110 (75.45%), Query Frame = 1 
Query:   70 GKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFS 399
            G+L+AVIGDEDTC GFLLGG+GE+D +R+ NF+VVE+DT    IE  F  F++R DI IILI Q  A+ IR  +D H  AVP VLEIPS+ HPYD S+DSILKRA+ + +
Sbjct:    9 GRLLAVIGDEDTCVGFLLGGIGEVDEDRETNFMVVERDTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVIT 118
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Match: Vha14-2 (gene:FBgn0037402 transcript:FBtr0309512)

HSP 1 Score: 137.887 bits (346), Expect = 6.167e-42
Identity = 66/110 (60.00%), Postives = 83/110 (75.45%), Query Frame = 1 
Query:   70 GKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFS 399
            G+L+AVIGDEDTC GFLLGG+GE+D +R+ NF+VVE+DT    IE  F  F++R DI IILI Q  A+ IR  +D H  AVP VLEIPS+ HPYD S+DSILKRA+ + +
Sbjct:    9 GRLLAVIGDEDTCVGFLLGGIGEVDEDRETNFMVVERDTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVIT 118
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Match: Vha14-2 (gene:FBgn0037402 transcript:FBtr0078585)

HSP 1 Score: 77.7962 bits (190), Expect = 2.000e-18
Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 1 
Query:  181 DTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAED 408
            DT    IE  F  F++R DI IILI Q  A+ IR  +D H  AVP VLEIPS+ HPYD S+DSILKRA+ + +  +
Sbjct:   51 DTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVITPPE 126
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Match: CG15719 (gene:FBgn0030440 transcript:FBtr0309878)

HSP 1 Score: 56.9954 bits (136), Expect = 2.933e-10
Identity = 26/87 (29.89%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:   79 IAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSR 339
            +A+I   +   GFLL GVG    +R  N+++VE +TP  D+E  F    +RS+I I++I  +  +++R ++ +    +P ++ +P++
Sbjct:   53 VAIIACPEVTLGFLLCGVG-YQKDRFRNYMMVESETPQEDVEQFFLTVYRRSNIGIVIIDYDTVKRLRTMMQRCSQLLPVLVTVPNK 138
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Zebrafish
Match: atp6v1f (ATPase H+ transporting V1 subunit F [Source:ZFIN;Acc:ZDB-GENE-040718-259])

HSP 1 Score: 181.03 bits (458), Expect = 7.210e-59
Identity = 84/117 (71.79%), Postives = 100/117 (85.47%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDTCTGFLLGG+GEL+  RKPNFLVVEK+T + +IE  F +F+ R+DI IILI Q IAE IRH +D H+ ++PAVLEIPS++HPYD SKDSIL+RAKGMFSAEDFR
Sbjct:    3 GRGKLIAVIGDEDTCTGFLLGGIGELNKNRKPNFLVVEKETSVTEIEETFKSFLARNDIGIILINQFIAEMIRHAIDAHMQSIPAVLEIPSKEHPYDASKDSILRRAKGMFSAEDFR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Xenopus
Match: uba6 (ubiquitin-like modifier activating enzyme 6 [Source:Xenbase;Acc:XB-GENE-1012392])

HSP 1 Score: 179.874 bits (455), Expect = 3.792e-52
Identity = 83/118 (70.34%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:   61 LAKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            LA+GKLIAVIGDEDTCTGFLLGG+GEL+  RKPNFLVVEK+T + +IE  F +F+ R DI IILI Q IAE IRH +D H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:    5 LARGKLIAVIGDEDTCTGFLLGGIGELNKNRKPNFLVVEKETSVTEIEETFRSFLNRDDIGIILINQFIAEMIRHAIDAHTVSIPAVLEIPSKEHPYDATKDSILRRAKGMFTAEDLR 122
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Mouse
Match: Atp6v1f (ATPase, H+ transporting, lysosomal V1 subunit F [Source:MGI Symbol;Acc:MGI:1913394])

HSP 1 Score: 170.629 bits (431), Expect = 7.759e-55
Identity = 80/117 (68.38%), Postives = 95/117 (81.20%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F  F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Mouse
Match: Atp6v1f (ATPase, H+ transporting, lysosomal V1 subunit F [Source:MGI Symbol;Acc:MGI:1913394])

HSP 1 Score: 95.5153 bits (236), Expect = 6.151e-26
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 1 
Query:  217 NFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:    6 QFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR 71
[Close]
BLAST of V-type proton ATPase subunit F vs. Ensembl Mouse
Match: Atp6v1f (ATPase, H+ transporting, lysosomal V1 subunit F [Source:MGI Symbol;Acc:MGI:1913394])

HSP 1 Score: 94.7449 bits (234), Expect = 2.630e-25
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 1 
Query:  217 NFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:   35 QFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR 100
[Close]
BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Match: sp|P31478|VATF_MANSE (V-type proton ATPase subunit F OS=Manduca sexta OX=7130 GN=VHA14 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.721e-54
Identity = 78/115 (67.83%), Postives = 97/115 (84.35%), Query Frame = 1 
Query:   67 KGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDF 411
            KGKLI+VIGDEDTC GFLLGG+GE++  R PNF+VV+K+TP+++IE  F  FVKR DI IILI QN+AE +RH++D H + VP+VLEIPS+DHPYD SKDSIL+RAKGMF+ ED 
Sbjct:    8 KGKLISVIGDEDTCVGFLLGGIGEINKNRHPNFMVVDKNTPVSEIEECFKRFVKRDDIDIILINQNVAELVRHVIDAHTAPVPSVLEIPSKDHPYDASKDSILRRAKGMFNPEDL 122
[Close]
BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Match: sp|O44091|VATF1_DROPS (V-type proton ATPase subunit F 1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=Vha14 PE=2 SV=2)

HSP 1 Score: 171.014 bits (432), Expect = 4.336e-54
Identity = 79/115 (68.70%), Postives = 97/115 (84.35%), Query Frame = 1 
Query:   67 KGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDF 411
            KGKLI+VIGDEDTC GFLLGGVGE++  R PNF+VV+K+TP++++E+ F  F+KR DI IILI QN AE IRH++D H S VPAVLEIPS+DHPYD SKDSIL+RA+GMF+ ED 
Sbjct:    8 KGKLISVIGDEDTCVGFLLGGVGEINKNRHPNFMVVDKNTPVSELEDCFKRFLKRDDIDIILINQNCAELIRHVIDAHTSPVPAVLEIPSKDHPYDASKDSILRRARGMFNPEDL 122
[Close]
BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Match: sp|P50408|VATF_RAT (V-type proton ATPase subunit F OS=Rattus norvegicus OX=10116 GN=Atp6v1f PE=1 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 5.427e-54
Identity = 80/117 (68.38%), Postives = 95/117 (81.20%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F  F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Match: sp|Q9D1K2|VATF_MOUSE (V-type proton ATPase subunit F OS=Mus musculus OX=10090 GN=Atp6v1f PE=1 SV=2)

HSP 1 Score: 170.629 bits (431), Expect = 5.427e-54
Identity = 80/117 (68.38%), Postives = 95/117 (81.20%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F  F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RAKGMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Match: sp|Q16864|VATF_HUMAN (V-type proton ATPase subunit F OS=Homo sapiens OX=9606 GN=ATP6V1F PE=1 SV=2)

HSP 1 Score: 170.244 bits (430), Expect = 8.133e-54
Identity = 79/117 (67.52%), Postives = 95/117 (81.20%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDT TGFLLGG+GEL+  R PNFLVVEKDT + +IE+ F  F+ R DI IILI Q IAE +RH LD H  ++PAVLEIPS++HPYD +KDSIL+RA+GMF+AED R
Sbjct:    3 GRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR 119
[Close]
BLAST of V-type proton ATPase subunit F vs. TrEMBL
Match: A0A183SXH2 (V-type proton ATPase subunit F OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS8920 PE=3 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 4.367e-60
Identity = 89/117 (76.07%), Postives = 104/117 (88.89%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            A+G+LIAVIGDEDTCTGFLLGG+GELD  R+PNFL V+KDTPL +IE AF +FVKR DIAIILIVQN+AE IRH+++ H + +PAVLEIPS+DHPYD +KDSILKRAKGMFS EDFR
Sbjct:    6 ARGRLIAVIGDEDTCTGFLLGGIGELDKNRRPNFLAVDKDTPLREIEEAFLSFVKREDIAIILIVQNVAEMIRHLIEAHTNPIPAVLEIPSKDHPYDSTKDSILKRAKGMFSVEDFR 122
[Close]
BLAST of V-type proton ATPase subunit F vs. TrEMBL
Match: A0A3P7LRE9 (V-type proton ATPase subunit F OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS10078 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.260e-60
Identity = 89/117 (76.07%), Postives = 105/117 (89.74%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            A+G+LIAVIGDEDTCTGFLLGG+GELD  R+PNFL V+KDTPL +IE AF +F+KR DIAIILIVQN+AE IRH++D H S +PA+LEIPS+DHPYD +KDSILKRAKGMFSA+DFR
Sbjct:    6 ARGRLIAVIGDEDTCTGFLLGGIGELDKNRRPNFLAVDKDTPLREIEEAFLSFIKREDIAIILIVQNVAEIIRHLIDSHTSPIPAILEIPSKDHPYDSNKDSILKRAKGMFSADDFR 122
[Close]
BLAST of V-type proton ATPase subunit F vs. TrEMBL
Match: A0A0R3UJF3 (V-type proton ATPase subunit F OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7636 PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.222e-59
Identity = 89/122 (72.95%), Postives = 106/122 (86.89%), Query Frame = 1 
Query:   55 MTLA--KGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            MT+A  +G+LIAVIGDEDTCTGFLLGG+GELD  R+PNFL V+KDTPL++IENAF +F+ R DIAIILIVQN+AE IRH++D +   +PAVLEIPS+D PYD SKDS+LKRAKGMFS EDFR
Sbjct:    1 MTVASSRGRLIAVIGDEDTCTGFLLGGIGELDRNRRPNFLAVDKDTPLSEIENAFTSFLSREDIAIILIVQNVAEMIRHLIDAYTEPIPAVLEIPSKDKPYDASKDSVLKRAKGMFSTEDFR 122
[Close]
BLAST of V-type proton ATPase subunit F vs. TrEMBL
Match: W6UFJ2 (V-type proton ATPase subunit F OS=Echinococcus granulosus OX=6210 GN=EGR_05550 PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.276e-59
Identity = 90/122 (73.77%), Postives = 106/122 (86.89%), Query Frame = 1 
Query:   55 MTLA--KGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            MT+A  +G+LIAVIGDEDTCTGFLLGG+GELD  R+PNFL V+KDTPL+DIENAF +F+ R DIAIILIVQN+AE IR+++D H   +PAVLEIPS+D PYD +KDSILKRAKGMFS EDFR
Sbjct:    1 MTVASSRGRLIAVIGDEDTCTGFLLGGIGELDKNRRPNFLAVDKDTPLSDIENAFTSFLTRDDIAIILIVQNVAEMIRYLIDAHTEPIPAVLEIPSKDKPYDANKDSILKRAKGMFSTEDFR 122
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BLAST of V-type proton ATPase subunit F vs. TrEMBL
Match: A0A4S2LYU1 (V-type proton ATPase subunit F OS=Opisthorchis felineus OX=147828 GN=CRM22_003931 PE=3 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 8.758e-59
Identity = 88/120 (73.33%), Postives = 104/120 (86.67%), Query Frame = 1 
Query:   55 MTLAKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            ++ A+GKLIAVIGDEDTCTGFLLGG+GELD  R+PNF  V+KDT L+ IE AF +FV R D+AIILIVQ IAE IRH++D H SA+PAVLEIPS+D+PYD SKD+ILKRAKG+FSAEDFR
Sbjct:    3 ISAARGKLIAVIGDEDTCTGFLLGGIGELDKNRRPNFFAVDKDTSLSAIEEAFKSFVDRDDVAIILIVQTIAEMIRHLIDAHTSALPAVLEIPSKDNPYDASKDTILKRAKGLFSAEDFR 122
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BLAST of V-type proton ATPase subunit F vs. Ensembl Cavefish
Match: atp6v1f (ATPase H+ transporting V1 subunit F [Source:NCBI gene;Acc:103042149])

HSP 1 Score: 184.496 bits (467), Expect = 1.991e-60
Identity = 86/117 (73.50%), Postives = 100/117 (85.47%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDTCTGFLLGG+GEL+  RKPNFLVVEKDT + +IE  F +F+ RSDI IILI Q IAE IRH +D H+ ++PAVLEIPS++HPYD SKDSIL+RAKGMFSAEDFR
Sbjct:    3 GRGKLIAVIGDEDTCTGFLLGGIGELNKNRKPNFLVVEKDTSITEIEETFKSFLARSDIGIILINQFIAEMIRHAIDAHMQSIPAVLEIPSKEHPYDASKDSILRRAKGMFSAEDFR 119
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BLAST of V-type proton ATPase subunit F vs. Ensembl Yeast
Match: VMA7 (Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits [Source:SGD;Acc:S000003252])

HSP 1 Score: 111.694 bits (278), Expect = 1.288e-32
Identity = 60/113 (53.10%), Postives = 79/113 (69.91%), Query Frame = 1 
Query:   67 KGKLIAVIGDEDTCTGFLLGGVGELDNE-RKPNFLVV-EKDTPLADIENAFHNFVK-RSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMF 396
            K  LIAVI DEDT TG LL G+G++  E ++ NF V  E  T   +I + F++F + R DIAI+LI Q+IAE IR  +D   +A PA+LEIPS+DHPYDP KDS+LKR + +F
Sbjct:    4 KRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGKTTKEEITDKFNHFTEERDDIAILLINQHIAENIRARVDSFTNAFPAILEIPSKDHPYDPEKDSVLKRVRKLF 116
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BLAST of V-type proton ATPase subunit F vs. Ensembl Nematostella
Match: EDO49363 (V-type proton ATPase subunit F [Source:UniProtKB/TrEMBL;Acc:A7RG58])

HSP 1 Score: 181.03 bits (458), Expect = 2.264e-59
Identity = 82/117 (70.09%), Postives = 104/117 (88.89%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
            AKG+LIAVIGDEDTCTGFLLGG+GE++ +R+ NFLVV KDT +++IE AF  F+ R+DIAI+LI QNIAE+IRH++D +  A+PAVLEIPS++ PYDPSKDSIL+RAKG+FSAEDF+
Sbjct:   11 AKGRLIAVIGDEDTCTGFLLGGIGEVNAKRQKNFLVVHKDTSVSEIEKAFEQFINRADIAILLINQNIAEEIRHVIDAYEKAIPAVLEIPSKEQPYDPSKDSILRRAKGLFSAEDFK 127
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BLAST of V-type proton ATPase subunit F vs. Ensembl Medaka
Match: atp6v1f (ATPase H+ transporting V1 subunit F [Source:NCBI gene;Acc:101164034])

HSP 1 Score: 183.341 bits (464), Expect = 6.448e-60
Identity = 85/117 (72.65%), Postives = 100/117 (85.47%), Query Frame = 1 
Query:   64 AKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIENAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYDPSKDSILKRAKGMFSAEDFR 414
             +GKLIAVIGDEDTCTGFLLGG+GEL+  RKPNFLVVEKDT + +IE  F +F+ R+DI IILI Q IAE IRH +D H+ ++PAVLEIPS++HPYD SKDSIL+RAKGMFSAEDFR
Sbjct:    3 GRGKLIAVIGDEDTCTGFLLGGIGELNKNRKPNFLVVEKDTSVTEIEETFRSFLARNDIGIILINQFIAEMIRHAIDAHVQSIPAVLEIPSKEHPYDASKDSILRRAKGMFSAEDFR 119
The following BLAST results are available for this feature:
BLAST of V-type proton ATPase subunit F vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ATP6V1F1.694e-5467.52ATPase H+ transporting V1 subunit F [Source:HGNC S... [more]
ATP6V1F4.486e-4854.48ATPase H+ transporting V1 subunit F [Source:HGNC S... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
vha-97.565e-5364.71Probable V-type proton ATPase subunit F [Source:U... [more]
vha-97.565e-5364.71Probable V-type proton ATPase subunit F [Source:U... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vha14-16.434e-5467.83gene:FBgn0262512 transcript:FBtr0087341[more]
Vha14-26.167e-4260.00gene:FBgn0037402 transcript:FBtr0309511[more]
Vha14-26.167e-4260.00gene:FBgn0037402 transcript:FBtr0309512[more]
Vha14-22.000e-1852.63gene:FBgn0037402 transcript:FBtr0078585[more]
CG157192.933e-1029.89gene:FBgn0030440 transcript:FBtr0309878[more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp6v1f7.210e-5971.79ATPase H+ transporting V1 subunit F [Source:ZFIN;A... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
uba63.792e-5270.34ubiquitin-like modifier activating enzyme 6 [Sourc... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Atp6v1f7.759e-5568.38ATPase, H+ transporting, lysosomal V1 subunit F [S... [more]
Atp6v1f6.151e-2666.67ATPase, H+ transporting, lysosomal V1 subunit F [S... [more]
Atp6v1f2.630e-2566.67ATPase, H+ transporting, lysosomal V1 subunit F [S... [more]
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BLAST of V-type proton ATPase subunit F vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P31478|VATF_MANSE3.721e-5467.83V-type proton ATPase subunit F OS=Manduca sexta OX... [more]
sp|O44091|VATF1_DROPS4.336e-5468.70V-type proton ATPase subunit F 1 OS=Drosophila pse... [more]
sp|P50408|VATF_RAT5.427e-5468.38V-type proton ATPase subunit F OS=Rattus norvegicu... [more]
sp|Q9D1K2|VATF_MOUSE5.427e-5468.38V-type proton ATPase subunit F OS=Mus musculus OX=... [more]
sp|Q16864|VATF_HUMAN8.133e-5467.52V-type proton ATPase subunit F OS=Homo sapiens OX=... [more]
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BLAST of V-type proton ATPase subunit F vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183SXH24.367e-6076.07V-type proton ATPase subunit F OS=Schistocephalus ... [more]
A0A3P7LRE95.260e-6076.07V-type proton ATPase subunit F OS=Dibothriocephalu... [more]
A0A0R3UJF31.222e-5972.95V-type proton ATPase subunit F OS=Mesocestoides co... [more]
W6UFJ21.276e-5973.77V-type proton ATPase subunit F OS=Echinococcus gra... [more]
A0A4S2LYU18.758e-5973.33V-type proton ATPase subunit F OS=Opisthorchis fel... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp6v1f1.991e-6073.50ATPase H+ transporting V1 subunit F [Source:NCBI g... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of V-type proton ATPase subunit F vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
VMA71.288e-3253.10Subunit F of the V1 peripheral membrane domain of ... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO493632.264e-5970.09V-type proton ATPase subunit F [Source:UniProtKB/... [more]
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BLAST of V-type proton ATPase subunit F vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp6v1f6.448e-6072.65ATPase H+ transporting V1 subunit F [Source:NCBI g... [more]
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BLAST of V-type proton ATPase subunit F vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005719 ID=SMED30005719|Name=V-type proton ATPase subunit F|organism=Schmidtea mediterranea sexual|type=transcript|length=560bp
ATTTAGTCAAATTTCAATTGTGAATTCAACAAATAATTCCGTTATTTTAA
AGAAATGACTTTAGCAAAAGGTAAATTAATTGCGGTTATTGGAGATGAAG
ATACGTGTACAGGATTTCTTCTTGGGGGTGTTGGTGAACTCGATAATGAG
AGAAAACCGAATTTTTTAGTCGTTGAAAAGGATACACCCTTAGCTGATAT
TGAAAATGCGTTTCATAACTTTGTTAAGCGTTCCGACATTGCTATAATTT
TGATTGTACAAAATATCGCGGAACAGATTCGACATATTTTGGATAAGCAT
ATATCAGCAGTGCCCGCTGTATTGGAAATACCGAGTCGGGATCATCCATA
TGATCCAAGTAAAGATTCTATTCTTAAAAGAGCAAAGGGAATGTTTTCGG
CAGAAGATTTCCGTTAAGTTCTCCTAAGAAATGTGTTGAACTAAGCATAA
TATATTTTGTCATTAATAAATGTTATTGACTTATTTTAAACGGAATTTGT
AATTTTTTATGTAAAAGACTTACATAATAACTGATATGAGGTTATACTTG
ACAGTTCAAA
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protein sequence of SMED30005719-orf-1

>SMED30005719-orf-1 ID=SMED30005719-orf-1|Name=SMED30005719-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=121bp
MTLAKGKLIAVIGDEDTCTGFLLGGVGELDNERKPNFLVVEKDTPLADIE
NAFHNFVKRSDIAIILIVQNIAEQIRHILDKHISAVPAVLEIPSRDHPYD
PSKDSILKRAKGMFSAEDFR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:1902600hydrogen ion transmembrane transport
GO:0034220ion transmembrane transport
GO:0015991ATP hydrolysis coupled proton transport
GO:0006810transport
GO:0006811ion transport
GO:0015992proton transport
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR008218ATPase_V1-cplx_f_g_su
IPR005772ATPase_V1-cplx_fsu_euk
Vocabulary: cellular component
TermDefinition
GO:0033180proton-transporting V-type ATPase, V1 domain
Vocabulary: molecular function
TermDefinition
GO:0046961proton-transporting ATPase activity, rotational mechanism