SMED30005706

Overview
NameSMED30005706
Smed IDSMED30005706
Length (bp)1199
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005706 (SMED30005706) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005706 (SMED30005706) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005706 (SMED30005706) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005706 (SMED30005706) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005706

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30005706h1SMcG0000031 Contig3155GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005706h1SMcG0015179 Contig3155GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005706h1SMcG0015181 Contig3155GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005706h1SMcG0015180 Contig3155GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30005706h1SMcG0015179 dd_Smed_v4_5445_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005706 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002001:24403..26378 +5858
Homology
[Close]
BLAST of SMED30005706 vs. TrEMBL
Match: A0A1D1VT88 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_12771 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.791e-10
Identity = 47/190 (24.74%), Postives = 90/190 (47.37%), Query Frame = 3 
Query:  309 NGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSS------QYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWP-IIAILAATLLFYSCSTICHETNQIQIYNQSRVV 857
            +GI C + +  SD  ++V ++  ++T++W+++NC +CF + + S      +Y+  +  F RKHQ   +C     N N    D       N +     C EC   Y D+   +L+   ++ S  C DV +L+   + ++ + F C   +P    +W  +I I ++T++FY             I  Q+R++
Sbjct:  159 DGISCQDVVYGSDLIEVVDRINTSITALWDNANCAYCFNSYSISHGTVVYEYSENLITFTRKHQNLSECF----NHNGIKLDRLLNKMENLTNATIVCEECYPMYKDLTDFFLKNYTEDISRTCMDVVDLMNQTRRMWSRYFRCQDRNPDMFYVWTVVIVIFSSTVVFYIAMLFNSTFEAYVIRGQARLL 344
[Close]
BLAST of SMED30005706 vs. TrEMBL
Match: A0A1D1VKM3 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_12771 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.875e-10
Identity = 44/169 (26.04%), Postives = 84/169 (49.70%), Query Frame = 3 
Query:  309 NGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSS------QYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWP-IIAILAATLLFY 794
            +GI C + +  SD  ++V ++  ++T++W+++NC +CF + + S      +Y+  +  F RKHQ   +C     N N    D       N +     C EC   Y D+   +L+   ++ S  C DV +L+   + ++ + F C   +P    +W  +I I ++T++FY
Sbjct:  159 DGISCQDVVYGSDLIEVVDRINTSITALWDNANCAYCFNSYSISHGTVVYEYSENLITFTRKHQNLSECF----NHNGIKLDRLLNKMENLTNATIVCEECYPMYKDLTDFFLKNYTEDISRTCMDVVDLMNQTRRMWSRYFRCQDRNPDMFYVWTVVIVIFSSTVVFY 323
[Close]
BLAST of SMED30005706 vs. TrEMBL
Match: A0A1D2M7Y7 (Osteopetrosis-associated transmembrane protein 1 OS=Orchesella cincta OX=48709 GN=Ocin01_17641 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.388e-6
Identity = 64/292 (21.92%), Postives = 108/292 (36.99%), Query Frame = 3 
Query:  126 QNLIQNENQCKLTTKRMSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLT----SSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNC-PKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFG---PDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDN 977
             ++I   + CK     +   +SN T C + NA P   C  C  NY   +    +F       G  C E     D+ ++VS   K +  +W    C  CF  L     ++     V  FV  ++    C   + N+NI+              N T C  C   Y+ M + Y+E+ +     VC DV + +   + ++ + F C P+    F        +    L+FY  +       + ++  Q R+   F      G S      +S++ +  +     P   PS   DN
Sbjct:   32 DDIITPSDVCKEIVALIGSAISNFTLCSVTNARPIHLCEWCVENYIRVVEVHRDFLAKTDEAGNPCKEYFLNLDRVEIVSDAYKYVEDLWAKGKCDSCFRKLDNGTLTNNATEEVRQFVTDYERTKGCFDDWLNANIT------------GLNQTACDACKPYYDQMNNKYMEIGELYGENVCVDVLDTMNITRELWSELFQCVPERKAEFWLHIGFGIVATIPLIFYFLAYALSTKQEARVLQQHRLAEVFANTSASGMSDLSGYHSSRLQESTTRSRSMPTTEPSDNCDN 311
[Close]
BLAST of SMED30005706 vs. TrEMBL
Match: V4A2A8 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_166554 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 6.100e-6
Identity = 59/273 (21.61%), Postives = 117/273 (42.86%), Query Frame = 3 
Query:  153 CKLTTKRMSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYN----PLVEDFVRKHQLFMDCLQKYTNSNISLCDST---DFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNC--------PKLSPGF-MNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQH 923
            C+   +  +E  SN T CLI+NA P      C     ++   +  +  ++     +C +AL  +D+ ++++ +  NM +VW++++C+ CF  +   +       L  D +  H  F          N+S+C +            +N + C +C   Y+ + S+Y ++ K+    VC D+ +++   + ++ K  NC        P L   F M + P+I        FY+ + I    ++  I  Q R+     P     +G++ +    QH
Sbjct:   61 CQAYLQMFAESASNFTECLILNARP---FRFCESCVVHYTKARGVYRDLFERKDKECHDALLKADRIQVLTLVYNNMLNVWSNADCENCFETIVEGEDGDVNFTLTNDTILFHNYF---------KNVSVCLNVTPGSIIDTEHQWNSSDCIKCKKSYSALNSHYEKIVKETSYHVCMDIIDMMNYTRLMWGKMLNCSHRQGDEAPVLGIAFVMGMVPVI--------FYTANRIFGAKHEKSIVVQKRLKK---PSSVPDYGSNYSRNSTQH 310
[Close]
BLAST of SMED30005706 vs. TrEMBL
Match: A0A194R1M0 (Osteopetrosis-associated transmembrane protein 1 OS=Papilio machaon OX=76193 GN=RR48_13385 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 9.617e-6
Identity = 66/241 (27.39%), Postives = 108/241 (44.81%), Query Frame = 3 
Query:  150 QCKLTTKRMSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKH-QLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCS--EVCGDVENLLLCAQTVYEKRFNCPKL--SPGFMNLWPIIAILAATLLFYSCSTICHETNQI-QIYNQSRV 854
            +C    +  S+  SN+T C I+NA P   C  C  +Y  F N+  E  K   VNG  C     + D+  +V +   ++++VW   NC  CF N T ++  P +++ +    +LF D +  ++N+             +   N T C +C   Y  + S Y  L  D+     VC D+ + +   ++++ K FNC KL  SP  + L     I    L+FY     C     + Q+  QSR+
Sbjct:    7 ECYKILESFSQSASNLTMCSILNARPVRLCEKCVDHYVCFRNKYQELLKT-VVNGTSCRSVFMSHDRLNVVLQYHDSISAVWEQGNCNNCF-NWTDNK--PAIKNDINHFIKLFNDTMNCFSNN------------YDPENNNTVCEKCMQFYLQLDSFYATLSADSMGLDSVCMDIVDSMNATRSIWSKTFNCCKLRRSPEIVFLSSAGVIALLPLIFYISVRYCGPIRDLPQVLKQSRI 231
[Close]
BLAST of SMED30005706 vs. Ensembl Medaka
Match: ostm1 (osteoclastogenesis associated transmembrane protein 1 [Source:NCBI gene;Acc:101155979])

HSP 1 Score: 48.1358 bits (113), Expect = 6.679e-6
Identity = 44/182 (24.18%), Postives = 73/182 (40.11%), Query Frame = 3 
Query:  321 CIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAI----LAATLLFYSCSTICHETNQIQIYNQSRV 854
            C ++L  SD+  LV  +  NM  +W  SNC FC T       N     F++     + C +KY   N                +   C  C   Y  +   Y  + K     +C DVE+ +   + ++ K FNC  +  G     P+IA+    L   ++FY  S +  E  + ++ +  R+
Sbjct:  130 CRDSLMRSDRLMLVYVLFSNMQDLWEKSNCDFCITEKFQGVNNETTH-FMKNLNQTLSCFEKYQQGN----------------HTELCKNCKSSYKGLNELYSSMEK----TLCIDVEDAMNMTRILWSKNFNC-SMPRG--ETVPVIAVSSFMLFLPIIFYLSSFLHSEQKKRKLIHPKRM 287
[Close]
BLAST of SMED30005706 vs. Planmine SMEST
Match: SMESG000021034.1 (SMESG000021034.1)

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 338/339 (99.71%), Postives = 339/339 (100.00%), Query Frame = 3 
Query:   69 MYISVFSLNLILLTSSVYSQNLIQNENQCKLTTKRMSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 1085
            MYISVFSLNLILLTSSVYSQNLIQNENQCKLTTKRMSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELR+DNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD
Sbjct:    1 MYISVFSLNLILLTSSVYSQNLIQNENQCKLTTKRMSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRQDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 339
[Close]
BLAST of SMED30005706 vs. Planmine SMEST
Match: SMESG000021034.1 (SMESG000021034.1)

HSP 1 Score: 595.119 bits (1533), Expect = 0.000e+0
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 3 
Query:  174 MSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 1085
            MSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELR+DNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD
Sbjct:    1 MSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRQDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 304
[Close]
BLAST of SMED30005706 vs. Planmine SMEST
Match: SMESG000021036.1 (SMESG000021036.1)

HSP 1 Score: 592.808 bits (1527), Expect = 0.000e+0
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 3 
Query:  174 MSELLSNITSCLIVNAGPKSTXXXXXXXXXXXXXEKSEFDKIWAVNGIKCIEALETSDKYKLVSKMLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 1085
            MSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSK LKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELR+DNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD
Sbjct:    1 MSELLSNITSCLIVNAGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSKRLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRQDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 304
[Close]
BLAST of SMED30005706 vs. Planmine SMEST
Match: SMESG000021035.1 (SMESG000021035.1)

HSP 1 Score: 239.58 bits (610), Expect = 4.404e-79
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 3 
Query:  744 MNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 1085
            MNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD
Sbjct:    1 MNLWPIIAILAATLLFYSCSTICHETNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYESDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD 114
[Close]
BLAST of SMED30005706 vs. Planmine SMEST
Match: SMESG000014644.1 (SMESG000014644.1)

HSP 1 Score: 59.6918 bits (143), Expect = 7.053e-10
Identity = 40/174 (22.99%), Postives = 80/174 (45.98%), Query Frame = 3 
Query:  393 WNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNSNISLCD-STDFPYRNASYNMTGCSECSVKYNDMLSNYLEL--------RKDNCSEVCGDVENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYS-CSTICHETNQIQIYNQSRVVACFGPDGNS 884
            W   NC+ C+    S +++     F+      M CLQ +TN++++  +   +  +    ++++ C  C+  Y+ + S Y  +        R+   + VCGD+ +++   + ++ K F C       +   P+  ++  TLLFY   ST  H+  ++  Y Q R+      +GNS
Sbjct:   80 WTDGNCEHCYKK-DSPEFSVDYTRFIELENSAMSCLQVFTNASLNFSNPKFETIWSGFGFDVSACRACNANYSAVNSFYTYMQEKVTGSDRQLLWNGVCGDIGDIMNTTRYLWSKEFKCDNSQVNLIFSLPLFIVIVVTLLFYCLASTYFHDKMRVVFYKQRRLKYMSNDNGNS 252
The following BLAST results are available for this feature:
BLAST of SMED30005706 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1D1VT886.791e-1024.74Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A1D1VKM36.875e-1026.04Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A1D2M7Y71.388e-621.92Osteopetrosis-associated transmembrane protein 1 O... [more]
V4A2A86.100e-621.61Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A194R1M09.617e-627.39Osteopetrosis-associated transmembrane protein 1 O... [more]
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BLAST of SMED30005706 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005706 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ostm16.679e-624.18osteoclastogenesis associated transmembrane protei... [more]
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BLAST of SMED30005706 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000021034.10.000e+099.71SMESG000021034.1[more]
SMESG000021034.10.000e+099.67SMESG000021034.1[more]
SMESG000021036.10.000e+099.34SMESG000021036.1[more]
SMESG000021035.14.404e-79100.00SMESG000021035.1[more]
SMESG000014644.17.053e-1022.99SMESG000014644.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005706 ID=SMED30005706|Name=SMED30005706|organism=Schmidtea mediterranea sexual|type=transcript|length=1199bp
GATCTATTATTTTGTAATAGTATACAGATTTATTTGATTGTGACTTTTTT
TCTATATACATTTCTTAAATGTATATTTCAGTTTTTTCTCTTAATTTGAT
TTTATTGACTTCGTCAGTTTATTCTCAAAATTTGATTCAAAATGAAAATC
AATGTAAACTAACAACTAAAAGGATGAGTGAGTTATTATCAAATATTACA
AGTTGTTTAATTGTAAATGCGGGACCAAAATCAACTTGTTTGAATTGCAA
TGTTAATTATAACAACTTTTTGAATGAAAAATCAGAATTCGATAAAATTT
GGGCGGTTAATGGAATAAAGTGTATCGAAGCACTTGAAACTTCTGATAAA
TATAAATTAGTTTCTAAAATGTTGAAAAATATGACAAGTGTTTGGAATCA
TTCGAATTGTCAATTCTGTTTTACAAATTTGACATCATCTCAATATAATC
CATTAGTAGAAGATTTTGTTAGAAAGCACCAATTGTTTATGGATTGTCTA
CAGAAATATACTAACTCTAATATTTCTTTATGTGATAGTACAGATTTTCC
ATATCGAAATGCTTCGTATAACATGACAGGCTGTTCAGAATGTTCTGTTA
AATACAATGATATGTTGAGCAATTATTTGGAGTTACGCAAAGACAATTGC
TCGGAAGTGTGTGGAGATGTCGAGAACCTGTTATTGTGTGCCCAAACAGT
GTATGAAAAACGTTTCAATTGCCCCAAATTGAGTCCAGGCTTCATGAATC
TATGGCCGATTATAGCAATCCTTGCTGCGACCCTCCTGTTCTACAGCTGC
TCGACAATTTGCCATGAAACCAATCAAATACAAATCTACAATCAGTCTCG
AGTTGTCGCTTGTTTCGGGCCTGACGGCAATTCGATGTTTGGAACATCTC
TCACCTCTCAAATGCATCAGCACAATTCCAACATCATGTTGCCGCCGCCA
TACAATCCTAGCTACGAATCCGACAATGAAAATTCATTGGAAAATCCAGC
ACAAATCCGGAAACCTCAAATCACTTCGTCATCGTATCTTTCGGTTCCGA
CAGATCATCAGATCAAATCTTTTTCTATTCAAGACTAATTGAGTGCAAAA
ATCGATTTATGGCGTTGTTTGTATTGCGATTTCTATTTTTCGTTGAAGCA
GCATTTTTCTCAATGTTCAATATCAATTTGTCCTGAAAATTTCCTGAAG
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protein sequence of SMED30005706-orf-1

>SMED30005706-orf-1 ID=SMED30005706-orf-1|Name=SMED30005706-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=340bp
MYISVFSLNLILLTSSVYSQNLIQNENQCKLTTKRMSELLSNITSCLIVN
AGPKSTCLNCNVNYNNFLNEKSEFDKIWAVNGIKCIEALETSDKYKLVSK
MLKNMTSVWNHSNCQFCFTNLTSSQYNPLVEDFVRKHQLFMDCLQKYTNS
NISLCDSTDFPYRNASYNMTGCSECSVKYNDMLSNYLELRKDNCSEVCGD
VENLLLCAQTVYEKRFNCPKLSPGFMNLWPIIAILAATLLFYSCSTICHE
TNQIQIYNQSRVVACFGPDGNSMFGTSLTSQMHQHNSNIMLPPPYNPSYE
SDNENSLENPAQIRKPQITSSSYLSVPTDHQIKSFSIQD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR019172Osteopetrosis-assoc_TM_1
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019172Osteopetrosis-associated transmembrane protein 1 precursorPFAMPF09777OSTMP1coord: 29..262
e-value: 8.8E-23
score: 80.9
IPR019172Osteopetrosis-associated transmembrane protein 1 precursorPANTHERPTHR15644OSTEOPETROSIS ASSOCIATED TRANSMEMBRANE PROTEIN 1coord: 12..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 299..320
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.778
NoneNo IPR availableTMHMMTMhelixcoord: 225..247